John Medley's second wife was Margaret Hudson. Born in 1821 in Carlisle, Cumberland, she was the daughter of a Royal Navy Commander. She grew up in the village of Crossmead, which was in Medley's parish of St Thomas, Exeter and later became a hospital nurse, a profession which she followed for 20 years. She came to Canada in 1863, accompanying the Bishop when he returned from a visit to England. They were married on 16 June 1863 in St. Anne's Church on Campobello Island. Bishop Medley and his second wife had no children. Mrs. Medley died in Fredericton in 1905.
