It uses this property to transport protons through the inner mitochondrial membrane (IMM). Protons are taken up from the intermembrane space and after transport through the IMM, they are released again in the mitochondrial matrix. Dinoseb in the dissociated form is negatively charged, which causes it to move to the intermembrane space because of the electrochemical gradient that exists across the IMM. The negative charge is delocalized over the ring, increasing the anion's membrane permeability.
