The spider has primarily been found in sandy environments, but has been observed thriving in swamps and on plants. The species is a specialist hunter and preys on different types of termites, including members of the genera Macrotermes and Odontotermes. The spider also feeds on other prey like fruit flies and leafhoppers. The spider captures its prey by a process of grasping, holding, and injecting its captured prey with venom. It produces a specialised venom that is dedicated for its prey, unlike other species which produce more general-purpose venom. It shares a similar environment to Stenaelurillus modestus, but the two species do not seem to compete for food or space. Stenaelurillus guttiger has also been found foraging along with Habrocestum africanum and Langellurillus squamiger.
