In 1556, he painted the Triptych of the Conception, Birth and Childhood of the Virgin, also for the convent of São Bento de Castris. This work is now found in the Museum of Sacred Art in Evora. This work is among the last to have been identified as being by de Contreiras. Other works, held in the Rilvas and Alpoim Galvão collections, and other private collections, show how important de Contreiras was to Portuguese painting in the 16th century.
