Klingermans Run is considered by the Pennsylvania Department of Environmental Protection to be a High-Quality Coldwater Fishery, but was considered to be a Coldwater Fishery as late as 1997. The stream is considered by the Pennsylvania Fish and Boat Commission to be Class A Wild Trout Waters between its headwaters and its mouth. It is inhabited by brook trout, but they are the only species of fish in the stream. These trout range between and in length. Their biomass is 33.90 kilograms per hectare. Trout also naturally reproduce in the stream.
