On August 3, 2022, it was announced that Barlow had signed with the Toronto Argonauts. He soon after made his professional debut on August 12, 2022, against the Hamilton Tiger-Cats. In his next game, on August 26, 2022, also against the Tiger-Cats, he recorded his first sack when he tackled Dane Evans late in the fourth quarter. Barlow made his first start in the last game of the regular season on October 29, 2022, against the Montreal Alouettes where he had three defensive tackles. He played in 10 of the team's last 11 regular season games in 2022 where he had 18 defensive tackles, one special teams tackle, and one sack.
