Upon entering Arkansas County the route passes the A.M. Bohnert Rice Plantation Pump#2 Engine and through sectioned farmland before meeting US 165B in Gillett. US 165/AR 1 continue north to De Witt, with AR 1 breaking east and US 165 turning due west. The route has a junction with Highway 11 at Eldridge Corner after which US 165 turns due north. This segment of US 165 was formerly AR 11 and is preserved in part by the National Register of Historic Places as Old AR 11 – Kauffman Road. The route enters Stuttgart after an intersection with 22nd Street. US 165 skirts the eastern edge of the city as Park Avenue before it turns west to intersect US 63 and enter Prairie County. The route only has one junction in its within the county, Highway 343.
