The first professional "non-friendly" game was an official first round KNVB Cup tie between Ajax and Willem II on 21 September 2016. This match was the first match to include a "pitchside monitor". The pitchside monitor would allow the referee to review footage from the field. Based on VAR but not using the available pitchside monitor, a yellow card was turned into a red card and thus this was the first ever VAR based expulsion in a professional game.
