Using targeted mutagenesis of Tbx4 in the mouse, various abnormalities were observed in the development of the allantois. Choirioallantoic fusion fails to occur in embryos with the homozygous null allele resulting in death 10.5 days post coitus, embryos with the Tbx4-mutant gene were observed to have allantoises that were apoptotic, stunted, and displayed abnormal differentiation with endothelial cells resulting in the absence of vascular remodeling.
