Usually, the ribosome profiling technique is used to acquire the translatome information. Other methods are polysome profiling, full-length translating mRNA profiling (RNC-seq), and translating ribosome affinity purification (TRAP-seq). Unlike the transcriptome, the translatome is a more accurate approximation for estimating the expression level of some genes, since the correlation between the proteome and translatome is higher than the correlation between the transcriptome and proteome.
