Church of the Saint Queen Alexandra was built in 1904 at the Orthodox cemetery of Nakhichevan-on-Don to replace a wooden chapel there. For this reason, the size of the temple is relatively small: about 18 × 13 metres. Architect Vladimir Popov built the church in Russian Revival style. In 1910, there was constructed a belfry, and in 1920 ― a narthex. Church of Queen Alexandra was initially assigned to the Sofia Church (which hasn't preserved). During the Nazi occupation of the city at Alexandrian cemetery there were buried Romanian Orthodox soldiers. In 1955, on the north side of the temple, there was constructed a chapel in the name of the Saint Prince Alexander Nevsky ― in memory of the demolished church of Alexander Nevsky in Nakhichevan-on-Don. To celebrate the 1000th anniversary of the Christianization of Rus', the dome was gilded.
