In 1552, Beit Iksa was an inhabited village. Haseki Hürrem Sultan, the favourite wife of Suleiman the Magnificent, endowed the tax revenues of Beit Iksa to its Haseki Sultan Imaret in Jerusalem. Administratively, Beit Iksa belonged to the District of Jerusalem. During this time, as in later periods, the residents of the village cultivated the lands of Kharruba.
