For the 1954 season, A&I was finally inducted into the Lone Star Conference (LSC). By 1959, the Javelinas won the first in a long string of LSC championships. Since then, the school has remained a perennial conference powerhouse, winning 27 championships. During the years that the Lone Star Conference was a member of the National Association of Intercollegiate Athletics (NAIA), the Javelinas also picked up seven national championships. During the decade of the 1970s, the Javelinas won five NAIA national championships and went undefeated from the last game of 1973 through third game of 1977.
