The nearest depot, Bahnbetriebswerk Neustadt, was primarily responsible for the running and rolling stock used on the line. The steam locomotives used in the first years of operation, were those of classes T 4.I and T 4.II of the Palatinate Railway. They were working the Cuckoo Railway until the late 1930s. From 1927, the Prussian T 9 - designated by the Deutsche Reichsbahn as DRG Class 91 - could be found on the route and, later, its sub-type, the Class T 9.3 was a common sight. Some years later, the DRG Class 64 locomotives, which had been stabled in Neustadt since 1928, also worked the branch line. In the first half of the 1950s, classes 57.10 (formerly the Prussian G 10) and 74 (formerly the Prussian T 12) were also used. The latter was especially employed on shunting duties. On 22 August 1954, number 91 593 headed the last scheduled steam-hauled passenger train. On this day it was decorated with the words "Cuckoo's last journey" (Kuckucks letzte Fahrt).
