The reserve hosts many rare and endangered species, which thrive there thanks to the virtual absence of humans. They include European bison, Przewalski horse, Moose, Brown bear, Golden eagle, and White-tailed eagle. It is home to the world's largest population of the European pond turtle. According to PSRER administration, there are 7 reptile, 11 amphibian, 46 mammal, 213 bird and 25 fish species. Of those, 70 are listed in the International Red Book and the Red Book of the Republic of Belarus. Flora includes 1251 registered plants, which is more than two-thirds of the flora of the country, and 18 of the plant species are listed in the Red Book. It was found that voles have higher rates of cataracts, useful populations of bacteria on the wings of birds in the zone are lower, partial albinism is common among barn swallows, cuckoos have become less common. Serious animal mutations, though, happened only right after the accident.
