The 1926–27 KBUs Mesterskabsrække was the 39th season of the Copenhagen Football Championship since its establishment in 1889, and the 25th under the administration of Kjøbenhavns Boldspil-Union (KBU). The season was launched with two league matches on 29 August 1926 and the final round of regular league fixtures were played on 8 May 1927 with two matches. Boldklubben 1903 were the defending league champions from the 1925–26 KBUs season. The winner of the league would automatically qualify for the national championship final of the 1926–27 Landsfodboldturneringen played at the end of the regular season. All six teams in the league, including the winner of the 1926–27 KBUs A-række, also participated in the inaugural season of the new Danish Championship play-off structure, the 1927–28 Danmarksmesterskabsturneringen i Fodbold. The same six teams including both the winners and runners-up of the second-tier league, 1926–27 KBUs A-række automatically qualified for the 1927 edition of KBUs Pokalturnering.
