Antonio Maffei is featured as a minor antagonist in the video game Assassin's Creed II, in which he is depicted as a member of the Templar Order, who within the game's storyline are responsible for the Pazzi conspiracy. Rather than being executed in Florence after the failure of the plot, he flees to San Gimignano, where he is later assassinated by Ezio Auditore. Maffei also makes a minor appearance in the game's prequel film, Assassin's Creed Lineage, played by Shawn Baichoo.
