On December 8, 2014, Colabello was claimed off waivers by the Toronto Blue Jays. They designated him for assignment on February 4, 2015, and he was outrighted to the Triple-A Buffalo Bisons on February 11. Colabello batted .337 with 5 home runs and 18 RBI in April 2015, and was named the International League Player of the Month. On May 5, Colabello's contract was purchased by the Blue Jays. In his second game with the Blue Jays, Colabello recorded his first career four-hit game. On May 29, Colabello played his first game against his former team, the Minnesota Twins. In the ninth inning, he hit a tiebreaking two-run home run off closer Glen Perkins to help the Blue Jays win 6–4. Colabello continued his excellent batting on June 7, when he helped the Blue Jays sweep the Houston Astros by hitting a walk-off two-run single. With the hit, he also extended his career-high hitting streak to 17 games. He hit his first career triple on October 2.
