In 2016, live tests were performed on sheep at the CSIRO Centre for Environment and Life Sciences in Floreat, Western Australia. 29 merino-cross wethers were fed one of five dosage levels (0%, 0.5%, 1%, 2% or 3% dietary intake) and monitored over a 72-day feeding period. In dosages of 2%, methane emission reductions of up to 85% were recorded when compared to control sheep. The sheep given dosages of 0.5% recorded at least a 50% reduction in methane emissions. No evidence of microbial adaptation occurred over the 72 days of testing as methane was continually and consistently mitigated. Tissue examination showed no adverse effects on the overall health of the sheep.
