On 1 January 1865, Clark E. Royce was appointed colonel and Frederick E. Camp was appointed lieutenant colonel of the 29th USCI. The regiment served on the Bermuda Hundred front in Spring 1865. The unit held trenches in the area of Chafin's Farm and Fort Burnham. On 26 March 1865, Birney's division was ordered to cross the James River and march to Hatcher's Run on the left flank. On 1 April, Major General Philip Sheridan crushed a Confederate force in the Battle of Five Forks. The following day at dawn, the regiment participated in an assault that broke through the Confederate defenses. The 29th USCI marched into Petersburg singing John Brown's Body. The regiment participated in the Appomattox Campaign through 9 April 1865. During the campaign, the 29th USCI was led by Colonel Royce and the 3rd Brigade, 2nd Division, XXV Corps was commanded by Colonel William W. Woodward.
