The Master of the Třeboň Altarpiece follows on from the earlier panel painting of Master Theodoric of the 1360s and 1370s. He also followed on from the wall paintings of Karlštejn Castle, in particular the Apocalypse series in the Chapel of the Virgin Mary. It is possible that the anonymous Master of the Třeboň Altarpiece was initially active in Master Theodoric’s workshop, where he would have also learned about Franco-Flemish art through Nicholas Wurmser of Strasbourg. Colour, which predominated over drawing, became his chief means of expression. He achieved the deepening of colour tone through the gradual application of translucent layers.
