After graduating, Kipiani taught at the St. Mary's School in Khoni until 1901, when she decided to join her father in Belgium. She enrolled in the medical faculty at the University of Brussels in 1902. Within two years, Kipiani was expelled because of an inability to pay her tuition. After making a presentation which was well-received at the Sixth International Congress of Physiologists held in Brussels in 1904, the Polish scientist Józefa Joteyko invited Kipiani to work in her laboratory at the Solvay Institute of Physiology and paid for her tuition. The laboratory focused on studying physiology, and specifically those functions that provided power to living organisms. Kipiani began studying how sugar could be used to combat fatigue. At the time, Armand Gautier in Paris and Auguste Slosse in Brussels were studying the diets of workers in their respective cities to evaluate whether the nutritional level of the population was adequate to maintain the level of their industrial activities. Her aim in conducting the studies was to contribute to the understanding of how nutrition and physiology could be applied to improving society. Findings on a study promoting vegetarianism sparked international discussion in newspapers, like The New York Times, women's magazines such as, Life & Health, and a book, Good Health and How We Won It by Upton Sinclair. Kipiani completed her studies in the medical faculty and graduated in 1907.
