Cardullo was called up to the major leagues for the first time on August 26, 2016. He hit his first Major League home run against Casey Fien in the first game of a doubleheader against the Los Angeles Dodgers on August 31, 2016, his 29th birthday, and his first grand slam in the second game against Bud Norris. On December 2, 2016, Cardullo was non-tendered by the Rockies after hitting .214/.254/.411 with 2 home runs and 6 RBI in 27 games.
