Pseudomonas stutzeri is a Gram-negative, rod-shaped, non-spore-forming bacterium that is typically 1–3 micrometres long and 0.5–0.8 micrometres wide. It tests positive for both the catalase and oxidase tests. P. stutzeri grows optimally at a temperature of about 35 °C, making it a mesophilic organism, although it can grow at temperatures as low as 4 °C and as high as 44 °C. When grown on a lysogeny broth (LB) medium at 32 °C, this bacterium has a doubling time of about 53 minutes. As the temperature is decreased to approximately 28 °C, the doubling time gets longer and can become as high as 72 minutes. On an asparagine (Asn) minimal medium, however, P. stutzeri has a typical doubling time of about 34 minutes. Despite the differences in doubling time between the two media, P. stutzeri reaches its stationary phase around 10–11 hours after being inoculated, or introduced, into both media. P. stutzeri grows best in media containing 2% NaCl although it can tolerate a salinity (NaCl content) ranging of 1–5%. This bacterium prefers a neutral [[pH] (pH7), but it can grow at a pH as high as 9. P. stutzeri possesses both type IV pili and a polar flagellum, both of which help it to be motile.
