The main panel portrays the Crucifixion of Jesus, against a background of hills in the Umbrian countryside, with a view of Città di Castello in the distance. Jesus looks peaceful even as he is dying on the cross, crowned with thorns and clad only in a loincloth that has an unusual red colour. Above, a sun in gold leaf and moon in silver leaf appear together in the sky. Two angels with flowing robes and scrolling ribbons at their waists, one floating to either side of the cross, are using gold chalices similar to communion vessels to catch the blood dripping from Jesus' nail-pierced hands and spurting from the wound in his side. To the proper right (Jesus' left) kneels Mary Magdalene, with John the Evangelist standing behind her. To the proper left (Jesus' right) his mother Mary stands behind the kneeling Saint Jerome, who is holding a stone with which the hermit would piously beat his own chest.
