All of what is now NY 65, including the portion of the route north of modern NY 31, was state-maintained by 1926. In the 1930 renumbering of state highways in New York, NY 65 was assigned to an alignment extending from NY 254 (now US 20A) in Honeoye northward to NY 15 (now NY 96) in Brighton. The route followed the northernmost portion of what is now CR 37 from Honeoye to US 20 in West Bloomfield, from where it continued to Brighton on its current alignment.Automobile Legal Association (ALA) Automobile Green Book, 1930–31 and 1931–32 editions, (Scarborough Motor Guide Co., Boston, 1930 and 1931). The 1930–31 edition shows New York state routes prior to the 1930 renumbering The section of NY 65 south of US 20, which was not state-owned in 1926, was removed from the route . Farther north, ownership and maintenance of the portion of the route north of NY 31 was transferred to Monroe County by 1990.
