Various methods can be used to prepare niosomes, such as the ether injection method (EIM), hand shaking method (HSM), reverse phase evaporation method (REV), trans-membrane pH gradient, "bubble" method, microfluidization method, formation of niosomes from proniosomes (Proniosome Technology (PT)), thin-film hydration method (TFH), heating method (HM), freeze and thaw method (FAT), and dehydration rehydration method (DRM).
