In 1925 Adelheid Kofler moved with her family to Innsbruck. From the early 1930s, she helped her husband in his research at University of Innsbruck's pharmacognostics institute. Using her knowledge of mineralogy, she conducted studies on the behavior of mixed crystals during melting and crystallization. Together with her husband, she developed the Kofler hot microscope (thermomicroscope) and the Kofler hot bench. Much of her research was focused on polymorphism, collaborating with fellow researcher, Maria Kuhnert-Brandstätter.
