Lucrezia's mother, Giulia Santi was married once before she married Alfonso Quistelli. She was married to Alberto II Pio of Savoy, who was killed. She was widowed in 1532, then remarried to Alfonso Quistelli the following year. Following the death of Santi's father, her family worked in the households of Medici dukes. Alfonso Quistelli worked with Alessandro de'Medici. Alessandro de'Medici was the Duke of Florence and the fiscal auditor, Quistelli was given a position on the Duke's primary legal counsel.
