FM 407 begins in Wise County, at US 81 / US 287 in New Fairview. The route travels east into Denton County and into the city of Justin; FM 407 was the primary access route between the former US 77 and farms that were part of the original Icarian Colony, where the city of Justin now sits. In Justin the route is concurrent with FM 156 for approximately four blocks. It crosses I-35W in Northlake and US 377 in Argyle.
