Molgula manhattensis can live from the intertidal zone to a depth of 300 feet. They can attach to solid organisms or even rocks, pilings, buoys, or sand. The sea squirts can also live in a temperature range of about 50 °F to about 80 °F. They can live in water with a salinity of 33.270 to 36.231 PSS, with oxygen concentrations between 3.960 and 6.328 mL/L.
