Germaine died on 15 October 1536 in Llíria, Valencia, at the age of 48, probably due to an edema caused by obesity. She was buried in the Monasterio de San Miguel de los Reyes ("Saint Michael of the Kings Monastery"), which she had founded. Her third husband survived her by 14 years and remained Viceroy of Valencia until his death in 1550. He remarried to a widow, Mencía de Mendoza (1508–1554) in 1541 with whom he continued his patronage of arts.
