human IgG onto a 600-Ångström silver film, and used the assay to detect anti-human IgG in water solution. Unlike many other immunoassays, such as ELISA, an SPR immunoassay is label free in that a label molecule is not required for detection of the analyte. Additionally, the measurements on SPR can be followed real-time allowing the monitoring of individual steps in sequential binding events particularly useful in the assessment of for instance sandwich complexes.
