Solvents can be used to extract Nimbin out of Neem seeds kernels. The kernels are powdered and defatted with hexane and methanol. The product obtained is then filtered, concentrated under pressure and separated with ethyl acetate and water. The final crude is then purified by repeating different silica gel column chromatography with hexane and ethyl acetate (70:30) as a solvent. This method can obtain 600 mg of Nimbin out of 300 g of Neem seed kernels.
