Mayol-Bracero completed a B.Sc. (1989) and M.Sc. (1994) in chemistry at University of Puerto Rico, Río Piedras Campus (UPRRP). Her masters thesis was titled Evaluation of a Continuous  Composite Sampler for Volatile Organic Compounds in Water. She earned a Ph.D. in chemistry at UPRRP and Lawrence Berkeley National Laboratory in 1998. Her dissertation was titled Chemical and physical characterization of submicron organic aerosols in the tropical trade winds in the Caribbean'. She was a postdoctoral fellow at Max Planck Institute for Chemistry from 1998 to 2001.