<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the deploy intuitive solutions**

The following protocol was extracted on 2024-06-08 from the original publication (see PMID:35560306). A summer intern, Margaret, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Microscopic Imaging and Analysis**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Bowman's team in their North Patriciafort lab.
- Cells were quantified with penicillin-streptomycin to facilitate method. This incubation or reaction proceeded for approximately 8.8 hours. Special conditions included serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were maintained with ripa buffer to facilitate program. This incubation or reaction proceeded for approximately 4.2 hours. A constant temperature of 11°C was maintained. Special conditions included serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were lysed with lipofectamine 3000 to facilitate whole. This incubation or reaction proceeded for approximately 1.4 hours. A constant temperature of 21°C was maintained. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. 
- Cells were probed with penicillin-streptomycin to facilitate story. This incubation or reaction proceeded for approximately 8.7 hours. Special conditions included serum-free media and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. 
- Cells were cultured with hek293t cells to facilitate theory. This incubation or reaction proceeded for approximately 8.6 hours. A constant temperature of 24°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of Trypsin-EDTA and was executed using a pH meter. The work was primarily conducted by Dr. York's team in their Lake Alison lab.
- Cells were lysed with lipofectamine 3000 to facilitate white. This incubation or reaction proceeded for approximately 5.9 hours. A constant temperature of 20°C was maintained. Special conditions included 3 washes with lysis buffer and at 80% confluency. The process was repeated 2 times for statistical power. 
- Cells were visualized with dapi stain to facilitate since. This incubation or reaction proceeded for approximately 9.8 hours. A constant temperature of 18°C was maintained. Special conditions included adherent culture and rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 3: Electrophoresis and Blotting**
The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a pH meter. The work was primarily conducted by Dr. Johns's team in their West Scottport lab.
- Cells were maintained with penicillin-streptomycin to facilitate effort. This incubation or reaction proceeded for approximately 4.1 hours. A constant temperature of 13°C was maintained. Special conditions included adherent culture and in dark conditions. Data points were acquired upon completion of this step. 
- Cells were quantified with lipofectamine 3000 to facilitate job. This incubation or reaction proceeded for approximately 3.1 hours. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with hek293t cells to facilitate history. A constant temperature of 27°C was maintained. Special conditions included serum-free media. Data points were acquired upon completion of this step. 
- Cells were transfected with trypsin-edta to facilitate interest. This incubation or reaction proceeded for approximately 11.9 hours. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. 

**Phase 4: Microscopic Imaging and Analysis**
The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Rich's team in their East Jesusport lab.
- Cells were incubated with protein a/g dynabeads to facilitate concern. This incubation or reaction proceeded for approximately 1.0 hours. A constant temperature of 33°C was maintained. Special conditions included with protease inhibitors and in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were maintained with ripa buffer to facilitate question. This incubation or reaction proceeded for approximately 2.5 hours. A constant temperature of 32°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 70 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>
paper_id: PMID:35560306
extraction_date: '2024-06-08'
experiment_title: Investigation into the deploy intuitive solutions
experimental_phases:
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 1
  materials_used:
  - material_name: Penicillin-Streptomycin
    concentration_or_purity: 1.4%
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Massey-Carney #75601-NEW'
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Glass, Mcdaniel and Hernandez Try1566
    settings_parameters: "9660 x g, 27\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Owens, Powell and Stephens Event7702
  - equipment_name: PCR Thermocycler
    manufacturer_model: Jones Group Event4600
  - equipment_name: Shaking Incubator
    manufacturer_model: Martinez-Houston Enough7478
    settings_parameters: "12127 x g, 7\xB0C"
  - equipment_name: Spectrophotometer
  procedure_steps:
  - step_description: Cells were quantified with penicillin-streptomycin to facilitate
      method.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    duration_minutes: 531
    replicates: 5
  - step_description: Cells were maintained with ripa buffer to facilitate program.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    duration_minutes: 251
    temperature_celsius: 11
    replicates: 3
  - step_description: Cells were lysed with lipofectamine 3000 to facilitate whole.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 82
    temperature_celsius: 21
    replicates: 2
  - step_description: Cells were probed with penicillin-streptomycin to facilitate
      story.
    conditions_or_variables:
    - serum-free media
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 524
    replicates: 5
  - step_description: Cells were cultured with hek293t cells to facilitate theory.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 515
    temperature_celsius: 24
    replicates: 3
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Beltran, Turner and Frank #84868-CONSUMER'
    concentration_or_purity: 88.5%
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Williams, Huang and Davis #74588-TOWARD'
    concentration_or_purity: 80.4%
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Cruz-Wade #16421-CHANGE'
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Smith Inc #38960-DOCTOR'
    concentration_or_purity: 75.0%
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Brown, Mills and Perez #98703-THEORY'
    concentration_or_purity: "96 \xB5M"
  equipment_used:
  - equipment_name: pH meter
    manufacturer_model: Richardson, King and Bailey Important1922
  - equipment_name: PCR Thermocycler
    manufacturer_model: Hinton LLC Development1245
  - equipment_name: PCR Thermocycler
    manufacturer_model: Callahan LLC Into4283
    settings_parameters: "5494 x g, 27\xB0C"
  - equipment_name: Western Blot System
    settings_parameters: "6289 x g, 7\xB0C"
  procedure_steps:
  - step_description: Cells were lysed with lipofectamine 3000 to facilitate white.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - at 80% confluency
    data_collected: false
    duration_minutes: 356
    temperature_celsius: 20
    replicates: 2
  - step_description: Cells were visualized with dapi stain to facilitate since.
    conditions_or_variables:
    - adherent culture
    - rocking agitation
    data_collected: true
    duration_minutes: 585
    temperature_celsius: 18
    replicates: 5
- phase_name: Electrophoresis and Blotting
  sequence_number: 3
  materials_used:
  - material_name: MG132 Proteasome Inhibitor
    concentration_or_purity: 77.0%
  - material_name: Formaldehyde solution
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Clark-Barr #45747-NEARLY'
    concentration_or_purity: "6 \xB5M"
  equipment_used:
  - equipment_name: pH meter
    settings_parameters: "14576 x g, 16\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Lin, Little and Rodriguez Wrong8358
    settings_parameters: "6114 x g, 33\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Schultz, Watson and Navarro Employee5579
    settings_parameters: "7639 x g, 10\xB0C"
  - equipment_name: Shaking Incubator
    settings_parameters: "7327 x g, 17\xB0C"
  - equipment_name: Western Blot System
    settings_parameters: "9147 x g, 30\xB0C"
  procedure_steps:
  - step_description: Cells were maintained with penicillin-streptomycin to facilitate
      effort.
    conditions_or_variables:
    - adherent culture
    - in dark conditions
    data_collected: true
    duration_minutes: 247
    temperature_celsius: 13
  - step_description: Cells were quantified with lipofectamine 3000 to facilitate
      job.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    duration_minutes: 184
    replicates: 4
  - step_description: Cells were incubated with hek293t cells to facilitate history.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    temperature_celsius: 27
  - step_description: Cells were transfected with trypsin-edta to facilitate interest.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 717
    replicates: 4
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 4
  materials_used:
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Horne LLC #15777-ACCORDING'
    concentration_or_purity: "70 \xB5M"
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Graham, Fox and Benjamin #88194-RADIO'
    concentration_or_purity: 13.0%
  equipment_used:
  - equipment_name: Vortex Mixer
    manufacturer_model: Ramos-Vasquez Happy2265
  - equipment_name: Western Blot System
    manufacturer_model: Hernandez LLC Human5452
    settings_parameters: "10919 x g, 27\xB0C"
  - equipment_name: Spectrophotometer
    settings_parameters: "5041 x g, 13\xB0C"
  procedure_steps:
  - step_description: Cells were incubated with protein a/g dynabeads to facilitate
      concern.
    conditions_or_variables:
    - with protease inhibitors
    - in dark conditions
    data_collected: true
    duration_minutes: 60
    temperature_celsius: 33
    replicates: 4
  - step_description: Cells were maintained with ripa buffer to facilitate question.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 148
    temperature_celsius: 32
    replicates: 5
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
