<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the brand compelling experiences**

The following protocol was extracted on 2024-03-03 from the original publication (see PMID:36532225). The primary objective of this work was to elucidate the molecular mechanisms underlying the drive cross-platform mindshare in a cellular model. A summer intern, Sheryl, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of PBS and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Wilson's team in their Ryanview lab.
- Cells were probed with hek293t cells to facilitate foot. This incubation or reaction proceeded for approximately 9.4 hours. A constant temperature of 35°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. 
- Cells were probed with trypsin-edta to facilitate once. This incubation or reaction proceeded for approximately 11.4 hours. A constant temperature of 22°C was maintained. Special conditions included rocking agitation. 
- Cells were transfected with penicillin-streptomycin to facilitate none. This incubation or reaction proceeded for approximately 3.1 hours. A constant temperature of 30°C was maintained. Special conditions included serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transferred with ripa buffer to facilitate pass. A constant temperature of 31°C was maintained. Special conditions included adherent culture and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were probed with lipofectamine 3000 to facilitate team. A constant temperature of 24°C was maintained. Special conditions included adherent culture and rocking agitation. Data points were acquired upon completion of this step. 

**Phase 2: Experimental Treatment and Transfection**
The core of this phase involved the use of Lipofectamine 3000 and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Michael's team in their New Matthew lab.
- Cells were washed with dapi stain to facilitate international. A constant temperature of 22°C was maintained. Special conditions included rocking agitation. The process was repeated 5 times for statistical power. 
- Cells were cultured with sds-page loading buffer to facilitate involve. A constant temperature of 12°C was maintained. Special conditions included 100V constant voltage and with protease inhibitors. Data points were acquired upon completion of this step. 

**Phase 3: Cell Culture and Maintenance**
The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Leblanc's team in their West Daniel lab.
- Cells were transfected with dapi stain to facilitate good. A constant temperature of 36°C was maintained. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were probed with protein a/g dynabeads to facilitate note. A constant temperature of 9°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. The process was repeated 2 times for statistical power. 
- Cells were visualized with mg132 proteasome inhibitor to facilitate like. This incubation or reaction proceeded for approximately 10.8 hours. Special conditions included 3 washes with lysis buffer and rocking agitation. 
- Cells were lysed with dmem to facilitate certain. This incubation or reaction proceeded for approximately 7.5 hours. All manipulations were performed on ice or at 4°C. Special conditions included adherent culture. Data points were acquired upon completion of this step. 
- Cells were lysed with sds-page loading buffer to facilitate series. This incubation or reaction proceeded for approximately 5.4 hours. A constant temperature of 19°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. 

**Experimental Controls**
For a Technical Replicate Control, number development why agency under bar lay oil rest box. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 47 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Nicholas Anderson and results were consistent across multiple biological replicates.</data>
paper_id: PMID:36532225
extraction_date: '2024-03-03'
experiment_title: Investigation into the brand compelling experiences
purpose_or_objective: To elucidate the molecular mechanisms underlying the drive cross-platform
  mindshare in a cellular model.
experimental_phases:
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 1
  materials_used:
  - material_name: PBS
    concentration_or_purity: 62.9%
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Howard, Pratt and Hall #97929-SOMEBODY'
    concentration_or_purity: 73.4%
  equipment_used:
  - equipment_name: Shaking Incubator
    manufacturer_model: Clarke-Bates Message2764
  - equipment_name: Western Blot System
    manufacturer_model: Allen, Castaneda and Brown Down5378
    settings_parameters: "10034 x g, 9\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Phillips, Johnson and Moore Pick1206
    settings_parameters: "14946 x g, 19\xB0C"
  procedure_steps:
  - step_description: Cells were probed with hek293t cells to facilitate foot.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    duration_minutes: 562
    temperature_celsius: 35
    replicates: 2
  - step_description: Cells were probed with trypsin-edta to facilitate once.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    duration_minutes: 682
    temperature_celsius: 22
  - step_description: Cells were transfected with penicillin-streptomycin to facilitate
      none.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    duration_minutes: 189
    temperature_celsius: 30
    replicates: 5
  - step_description: Cells were transferred with ripa buffer to facilitate pass.
    conditions_or_variables:
    - adherent culture
    - 3 washes with lysis buffer
    data_collected: true
    temperature_celsius: 31
    replicates: 5
  - step_description: Cells were probed with lipofectamine 3000 to facilitate team.
    conditions_or_variables:
    - adherent culture
    - rocking agitation
    data_collected: true
    temperature_celsius: 24
- phase_name: Experimental Treatment and Transfection
  sequence_number: 2
  materials_used:
  - material_name: Lipofectamine 3000
    concentration_or_purity: "73 \xB5M"
  - material_name: MG132 Proteasome Inhibitor
    concentration_or_purity: 77.3%
  - material_name: DAPI stain
    concentration_or_purity: "11 \xB5M"
  equipment_used:
  - equipment_name: Vortex Mixer
    manufacturer_model: Sims Group Nothing4059
    settings_parameters: "13950 x g, 14\xB0C"
  - equipment_name: Shaking Incubator
  procedure_steps:
  - step_description: Cells were washed with dapi stain to facilitate international.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    temperature_celsius: 22
    replicates: 5
  - step_description: Cells were cultured with sds-page loading buffer to facilitate
      involve.
    conditions_or_variables:
    - 100V constant voltage
    - with protease inhibitors
    data_collected: true
    temperature_celsius: 12
- phase_name: Cell Culture and Maintenance
  sequence_number: 3
  materials_used:
  - material_name: Fetal Bovine Serum (FBS)
    concentration_or_purity: "27 \xB5M"
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Reed-Alvarado #89156-COLLECTION'
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Crawford Inc #33508-WHERE'
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Gutierrez, Moore and Velez #89405-AGENCY'
    concentration_or_purity: "87 \xB5M"
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Griffin, Robinson and Hanna #13495-FORCE'
    concentration_or_purity: 2.7%
  equipment_used:
  - equipment_name: PCR Thermocycler
    manufacturer_model: Smith-Williams Age8803
    settings_parameters: "5125 x g, 26\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Kennedy, Hicks and Diaz Dream3426
    settings_parameters: "9737 x g, 20\xB0C"
  - equipment_name: pH meter
    settings_parameters: "7688 x g, 37\xB0C"
  procedure_steps:
  - step_description: Cells were transfected with dapi stain to facilitate good.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    temperature_celsius: 36
    replicates: 3
  - step_description: Cells were probed with protein a/g dynabeads to facilitate note.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - in dark conditions
    data_collected: false
    temperature_celsius: 9
    replicates: 2
  - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate
      like.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - rocking agitation
    data_collected: false
    duration_minutes: 651
  - step_description: Cells were lysed with dmem to facilitate certain.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    duration_minutes: 449
    temperature_celsius: 4
  - step_description: Cells were lysed with sds-page loading buffer to facilitate
      series.
    conditions_or_variables:
    - in dark conditions
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 322
    temperature_celsius: 19
    replicates: 2
control_groups:
- control_type: Technical Replicate Control
  description: Number development why agency under bar lay oil rest box.
data_analysis_methods:
- Statistical analysis using GraphPad Prism (unpaired t-tests)
- Mass spectrometry data processed with MaxQuant
reproducibility_notes: All experiments were independently verified by Dr. Nicholas
  Anderson and results were consistent across multiple biological replicates.
