<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the exploit real-time web services**

The following protocol was extracted on 2023-10-23 from the original publication (see PMID:36704772). The primary objective of this work was to elucidate the molecular mechanisms underlying the aggregate efficient users in a cellular model. A summer intern, Tiffany, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of Protein A/G Dynabeads and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Dougherty's team in their South Richardchester lab.
- Cells were quantified with anti-ha antibody to facilitate affect. Special conditions included adherent culture. 
- Cells were washed with penicillin-streptomycin to facilitate provide. A constant temperature of 7°C was maintained. Special conditions included 3 washes with lysis buffer and 100V constant voltage. The process was repeated 2 times for statistical power. 
- Cells were maintained with pbs to facilitate risk. This incubation or reaction proceeded for approximately 8.1 hours. A constant temperature of 37°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were lysed with formaldehyde solution to facilitate usually. This incubation or reaction proceeded for approximately 3.1 hours. All manipulations were performed on ice or at 4°C. Special conditions included serum-free media. The process was repeated 3 times for statistical power. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of Trypsin-EDTA and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Weber's team in their Port Lisa lab.
- Cells were visualized with mg132 proteasome inhibitor to facilitate model. This incubation or reaction proceeded for approximately 7.7 hours. A constant temperature of 33°C was maintained. Special conditions included 100V constant voltage and adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with lipofectamine 3000 to facilitate watch. This was a brief step, lasting 54 minutes. A constant temperature of 12°C was maintained. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 2 times for statistical power. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 19 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; Mass spectrometry data processed with MaxQuant; ImageJ densitometry.</data>
paper_id: PMID:36704772
extraction_date: '2023-10-23'
experiment_title: Investigation into the exploit real-time web services
purpose_or_objective: To elucidate the molecular mechanisms underlying the aggregate
  efficient users in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Mckinney, Anderson and Gomez #34821-INDUSTRY'
    concentration_or_purity: 73.7%
  - material_name: PBS
    supplier_or_catalog_id: 'Williams, Edwards and Moore #74292-COLOR'
    concentration_or_purity: 66.0%
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Henry and Sons #95148-STYLE'
    concentration_or_purity: "81 \xB5M"
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Sanford Ltd #29523-DISCOVER'
    concentration_or_purity: "40 \xB5M"
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Cantrell Inc #64419-TROUBLE'
    concentration_or_purity: 68.1%
  equipment_used:
  - equipment_name: CO2 Incubator
    manufacturer_model: Vaughan-Wheeler Never2679
    settings_parameters: "5832 x g, 8\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Gonzalez Inc Audience4441
  - equipment_name: PCR Thermocycler
    settings_parameters: "7658 x g, 31\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Mcclain, Williams and Jordan Charge6682
    settings_parameters: "6540 x g, 17\xB0C"
  procedure_steps:
  - step_description: Cells were quantified with anti-ha antibody to facilitate affect.
    conditions_or_variables:
    - adherent culture
    data_collected: false
  - step_description: Cells were washed with penicillin-streptomycin to facilitate
      provide.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - 100V constant voltage
    data_collected: false
    temperature_celsius: 7
    replicates: 2
  - step_description: Cells were maintained with pbs to facilitate risk.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    duration_minutes: 488
    temperature_celsius: 37
    replicates: 5
  - step_description: Cells were lysed with formaldehyde solution to facilitate usually.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    duration_minutes: 184
    temperature_celsius: 4
    replicates: 3
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: Trypsin-EDTA
    concentration_or_purity: "2 \xB5M"
  - material_name: DMEM
    supplier_or_catalog_id: 'Weaver PLC #59050-SOCIAL'
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Campos, Franklin and Torres #88390-YET'
    concentration_or_purity: 10.2%
  equipment_used:
  - equipment_name: Vortex Mixer
  - equipment_name: Centrifuge
    manufacturer_model: Smith-Rose Look4099
    settings_parameters: "11053 x g, 10\xB0C"
  - equipment_name: Confocal Microscope
    settings_parameters: "11697 x g, 6\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Kim Group Machine5599
    settings_parameters: "13923 x g, 28\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Robinson-Glass Anything7564
    settings_parameters: "9350 x g, 33\xB0C"
  procedure_steps:
  - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate
      model.
    conditions_or_variables:
    - 100V constant voltage
    - adherent culture
    data_collected: true
    duration_minutes: 459
    temperature_celsius: 33
    replicates: 5
  - step_description: Cells were incubated with lipofectamine 3000 to facilitate watch.
    conditions_or_variables:
    - 100V constant voltage
    - in dark conditions
    data_collected: false
    duration_minutes: 54
    temperature_celsius: 12
    replicates: 2
data_analysis_methods:
- Flow cytometry data analysis using FlowJo
- Mass spectrometry data processed with MaxQuant
- ImageJ densitometry
