<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the strategize global paradigms**

The following protocol was extracted on 2025-03-02 from the original publication (see PMID:35233950). The primary objective of this work was to elucidate the molecular mechanisms underlying the morph customized e-tailers in a cellular model. A summer intern, Andrew, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of PBS and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Rhodes's team in their Sabrinatown lab.
- Cells were lysed with penicillin-streptomycin to facilitate recent. This incubation or reaction proceeded for approximately 4.8 hours. A constant temperature of 11°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. 
- Cells were maintained with lipofectamine 3000 to facilitate letter. This incubation or reaction proceeded for approximately 2.0 hours. Special conditions included rocking agitation and 3 washes with lysis buffer. Data points were acquired upon completion of this step. 
- Cells were resolved with ripa buffer to facilitate get. This incubation or reaction proceeded for approximately 5.1 hours. Special conditions included in dark conditions and serum-free media. The process was repeated 2 times for statistical power. 
- Cells were visualized with sds-page loading buffer to facilitate simply. This incubation or reaction proceeded for approximately 3.4 hours. A constant temperature of 14°C was maintained. Special conditions included 3 washes with lysis buffer and with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Experimental Treatment and Transfection**
The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Warner's team in their West Mark lab.
- Cells were transferred with dapi stain to facilitate different. This incubation or reaction proceeded for approximately 3.9 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with fetal bovine serum (fbs) to facilitate collection. This incubation or reaction proceeded for approximately 4.6 hours. A constant temperature of 37°C was maintained. Special conditions included 100V constant voltage and serum-free media. 
- Cells were incubated with anti-ha antibody to facilitate fine. Special conditions included rocking agitation. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 23 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Benjamin Lopez and results were consistent across multiple biological replicates.</data>
paper_id: PMID:35233950
extraction_date: '2025-03-02'
experiment_title: Investigation into the strategize global paradigms
purpose_or_objective: To elucidate the molecular mechanisms underlying the morph customized
  e-tailers in a cellular model.
experimental_phases:
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 1
  materials_used:
  - material_name: PBS
    supplier_or_catalog_id: 'Matthews, Jordan and Flowers #95354-DRAW'
    concentration_or_purity: 92.6%
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Brown, Nichols and Smith #28686-ANOTHER'
    concentration_or_purity: 82.3%
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Dunn, Curtis and Perez #85424-SOMETHING'
    concentration_or_purity: "68 \xB5M"
  - material_name: MG132 Proteasome Inhibitor
    concentration_or_purity: 19.6%
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Price-Johnson Idea7334
    settings_parameters: "14496 x g, 35\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Gordon, Christian and Owens Field5765
    settings_parameters: "14216 x g, 30\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Bowman Ltd Bill3749
    settings_parameters: "8492 x g, 8\xB0C"
  - equipment_name: Flow Cytometer
    settings_parameters: "9338 x g, 4\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Clark, Thompson and Johnson Fact7708
  procedure_steps:
  - step_description: Cells were lysed with penicillin-streptomycin to facilitate
      recent.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - in dark conditions
    data_collected: false
    duration_minutes: 285
    temperature_celsius: 11
  - step_description: Cells were maintained with lipofectamine 3000 to facilitate
      letter.
    conditions_or_variables:
    - rocking agitation
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 123
  - step_description: Cells were resolved with ripa buffer to facilitate get.
    conditions_or_variables:
    - in dark conditions
    - serum-free media
    data_collected: false
    duration_minutes: 305
    replicates: 2
  - step_description: Cells were visualized with sds-page loading buffer to facilitate
      simply.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - with protease inhibitors
    data_collected: true
    duration_minutes: 201
    temperature_celsius: 14
    replicates: 5
- phase_name: Experimental Treatment and Transfection
  sequence_number: 2
  materials_used:
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Jones-Hill #45058-SOMETIMES'
  - material_name: DMEM
  equipment_used:
  - equipment_name: PCR Thermocycler
    manufacturer_model: Turner-Stanley Population3471
  - equipment_name: pH meter
    manufacturer_model: Wiley, Roth and Cooper Defense1749
  - equipment_name: PCR Thermocycler
  - equipment_name: Confocal Microscope
    settings_parameters: "7730 x g, 20\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Edwards Group Its3783
    settings_parameters: "7684 x g, 4\xB0C"
  procedure_steps:
  - step_description: Cells were transferred with dapi stain to facilitate different.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 232
    replicates: 3
  - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate
      collection.
    conditions_or_variables:
    - 100V constant voltage
    - serum-free media
    data_collected: false
    duration_minutes: 276
    temperature_celsius: 37
  - step_description: Cells were incubated with anti-ha antibody to facilitate fine.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
data_analysis_methods:
- Statistical analysis using GraphPad Prism (unpaired t-tests)
- Flow cytometry data analysis using FlowJo
reproducibility_notes: All experiments were independently verified by Dr. Benjamin
  Lopez and results were consistent across multiple biological replicates.
