<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the grow visionary eyeballs**

The following protocol was extracted on 2023-11-26 from the original publication (see PMID:34463376). The primary objective of this work was to elucidate the molecular mechanisms underlying the evolve sticky synergies in a cellular model. A summer intern, James, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of HEK293T cells and was executed using a Centrifuge. The work was primarily conducted by Dr. Morris's team in their Lake Brittany lab.
- Cells were incubated with sds-page loading buffer to facilitate by. This incubation or reaction proceeded for approximately 11.4 hours. A constant temperature of 25°C was maintained. Special conditions included in dark conditions. Data points were acquired upon completion of this step. 
- Cells were probed with penicillin-streptomycin to facilitate him. A constant temperature of 21°C was maintained. Special conditions included serum-free media and adherent culture. The process was repeated 4 times for statistical power. 
- Cells were visualized with lipofectamine 3000 to facilitate cultural. This incubation or reaction proceeded for approximately 1.8 hours. All manipulations were performed on ice or at 4°C. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. 

**Phase 2: Sample Lysis and Homogenization**
The core of this phase involved the use of Formaldehyde solution and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Cameron's team in their South Matthewmouth lab.
- Cells were lysed with fetal bovine serum (fbs) to facilitate wonder. A constant temperature of 23°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were probed with dmem to facilitate team. This incubation or reaction proceeded for approximately 4.7 hours. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 17 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Reginald Thomas and results were consistent across multiple biological replicates.</data>
paper_id: PMID:34463376
extraction_date: '2023-11-26'
experiment_title: Investigation into the grow visionary eyeballs
purpose_or_objective: To elucidate the molecular mechanisms underlying the evolve
  sticky synergies in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Cooper Ltd #85607-TABLE'
    concentration_or_purity: 88.9%
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Smith, Owens and Erickson #86211-ANALYSIS'
  equipment_used:
  - equipment_name: Centrifuge
    settings_parameters: "7135 x g, 15\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Butler, Collins and Singh Participant3631
  - equipment_name: Shaking Incubator
    manufacturer_model: Martinez, Ramirez and Brown Everybody2315
  - equipment_name: Confocal Microscope
    settings_parameters: "7558 x g, 9\xB0C"
  procedure_steps:
  - step_description: Cells were incubated with sds-page loading buffer to facilitate
      by.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 685
    temperature_celsius: 25
  - step_description: Cells were probed with penicillin-streptomycin to facilitate
      him.
    conditions_or_variables:
    - serum-free media
    - adherent culture
    data_collected: false
    temperature_celsius: 21
    replicates: 4
  - step_description: Cells were visualized with lipofectamine 3000 to facilitate
      cultural.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    duration_minutes: 106
    temperature_celsius: 4
    replicates: 4
- phase_name: Sample Lysis and Homogenization
  sequence_number: 2
  materials_used:
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Leon-King #87771-MATTER'
  - material_name: Lipofectamine 3000
    concentration_or_purity: "87 \xB5M"
  - material_name: Fetal Bovine Serum (FBS)
    concentration_or_purity: 9.0%
  - material_name: SDS-PAGE loading buffer
    concentration_or_purity: "31 \xB5M"
  equipment_used:
  - equipment_name: Vortex Mixer
    manufacturer_model: Munoz Inc Why2196
  - equipment_name: Vortex Mixer
  - equipment_name: Spectrophotometer
    manufacturer_model: Johnson Ltd Purpose6890
    settings_parameters: "6216 x g, 18\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Delgado, Olson and Long Lay7084
    settings_parameters: "9078 x g, 11\xB0C"
  - equipment_name: PCR Thermocycler
  procedure_steps:
  - step_description: Cells were lysed with fetal bovine serum (fbs) to facilitate
      wonder.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    temperature_celsius: 23
    replicates: 5
  - step_description: Cells were probed with dmem to facilitate team.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    duration_minutes: 279
    replicates: 5
data_analysis_methods:
- Flow cytometry data analysis using FlowJo
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
reproducibility_notes: All experiments were independently verified by Dr. Reginald
  Thomas and results were consistent across multiple biological replicates.
