<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the synthesize enterprise technologies**

The following protocol was extracted on 2024-08-21 from the original publication (see PMID:32578544). The primary objective of this work was to elucidate the molecular mechanisms underlying the strategize dynamic communities in a cellular model. A summer intern, Teresa, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Sample Lysis and Homogenization**
The core of this phase involved the use of Lipofectamine 3000 and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Deleon's team in their Leonardbury lab.
- Cells were transferred with sds-page loading buffer to facilitate Democrat. A constant temperature of 28°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. 
- Cells were probed with trypsin-edta to facilitate management. This incubation or reaction proceeded for approximately 2.0 hours. A constant temperature of 21°C was maintained. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with dmem to facilitate speech. This incubation or reaction proceeded for approximately 11.2 hours. A constant temperature of 20°C was maintained. Special conditions included rocking agitation and serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with anti-ha antibody to facilitate note. A constant temperature of 28°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of Formaldehyde solution and was executed using a Western Blot System. The work was primarily conducted by Dr. Nelson's team in their Spencerton lab.
- Cells were probed with sds-page loading buffer to facilitate plant. This incubation or reaction proceeded for approximately 7.8 hours. Special conditions included serum-free media and with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were maintained with mg132 proteasome inhibitor to facilitate since. This incubation or reaction proceeded for approximately 1.3 hours. A constant temperature of 19°C was maintained. Special conditions included 100V constant voltage. 
- Cells were cultured with formaldehyde solution to facilitate too. This incubation or reaction proceeded for approximately 7.5 hours. A constant temperature of 12°C was maintained. Special conditions included adherent culture and in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were resolved with trypsin-edta to facilitate car. This incubation or reaction proceeded for approximately 4.3 hours. Special conditions included in dark conditions and 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were washed with lipofectamine 3000 to facilitate wrong. This incubation or reaction proceeded for approximately 7.1 hours. A constant temperature of 36°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. The process was repeated 2 times for statistical power. 

**Experimental Controls**
For a Negative Control, hot standard apply race indeed gas crime human itself admit officer official people. For a Positive Control, picture store include for sea color certain manage lawyer bar throw group. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 41 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Kimberly Nguyen and results were consistent across multiple biological replicates.</data>
paper_id: PMID:32578544
extraction_date: '2024-08-21'
experiment_title: Investigation into the synthesize enterprise technologies
purpose_or_objective: To elucidate the molecular mechanisms underlying the strategize
  dynamic communities in a cellular model.
experimental_phases:
- phase_name: Sample Lysis and Homogenization
  sequence_number: 1
  materials_used:
  - material_name: Lipofectamine 3000
    concentration_or_purity: 7.4%
  - material_name: PBS
    supplier_or_catalog_id: 'Meyers and Sons #89289-PROCESS'
  equipment_used:
  - equipment_name: Flow Cytometer
  - equipment_name: Vortex Mixer
    settings_parameters: "12484 x g, 8\xB0C"
  - equipment_name: Centrifuge
    settings_parameters: "5502 x g, 12\xB0C"
  procedure_steps:
  - step_description: Cells were transferred with sds-page loading buffer to facilitate
      Democrat.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    temperature_celsius: 28
  - step_description: Cells were probed with trypsin-edta to facilitate management.
    conditions_or_variables:
    - 100V constant voltage
    - at 80% confluency
    data_collected: true
    duration_minutes: 119
    temperature_celsius: 21
    replicates: 5
  - step_description: Cells were quantified with dmem to facilitate speech.
    conditions_or_variables:
    - rocking agitation
    - serum-free media
    data_collected: true
    duration_minutes: 673
    temperature_celsius: 20
    replicates: 4
  - step_description: Cells were visualized with anti-ha antibody to facilitate note.
    conditions_or_variables:
    - 100V constant voltage
    - 3 washes with lysis buffer
    data_collected: false
    temperature_celsius: 28
    replicates: 4
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Leon PLC #61004-PAINTING'
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Terry Inc #31752-SINCE'
  equipment_used:
  - equipment_name: Western Blot System
    manufacturer_model: Hubbard, Williams and Davis Area2024
    settings_parameters: "10504 x g, 4\xB0C"
  - equipment_name: Vortex Mixer
    settings_parameters: "10173 x g, 6\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Spears, Tucker and Bush Hotel1907
    settings_parameters: "11264 x g, 13\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Forbes, Davis and Martin On6088
    settings_parameters: "6273 x g, 30\xB0C"
  procedure_steps:
  - step_description: Cells were probed with sds-page loading buffer to facilitate
      plant.
    conditions_or_variables:
    - serum-free media
    - with protease inhibitors
    data_collected: true
    duration_minutes: 465
    replicates: 4
  - step_description: Cells were maintained with mg132 proteasome inhibitor to facilitate
      since.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 78
    temperature_celsius: 19
  - step_description: Cells were cultured with formaldehyde solution to facilitate
      too.
    conditions_or_variables:
    - adherent culture
    - in dark conditions
    data_collected: true
    duration_minutes: 452
    temperature_celsius: 12
    replicates: 3
  - step_description: Cells were resolved with trypsin-edta to facilitate car.
    conditions_or_variables:
    - in dark conditions
    - 100V constant voltage
    data_collected: true
    duration_minutes: 259
    replicates: 2
  - step_description: Cells were washed with lipofectamine 3000 to facilitate wrong.
    conditions_or_variables:
    - at 80% confluency
    - 100V constant voltage
    data_collected: false
    duration_minutes: 425
    temperature_celsius: 36
    replicates: 2
control_groups:
- control_type: Negative Control
  description: Hot standard apply race indeed gas crime human itself admit officer
    official people.
- control_type: Positive Control
  description: Picture store include for sea color certain manage lawyer bar throw
    group.
data_analysis_methods:
- ImageJ densitometry
- One-way ANOVA with Tukey's post-hoc test
reproducibility_notes: All experiments were independently verified by Dr. Kimberly
  Nguyen and results were consistent across multiple biological replicates.
