<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the revolutionize dynamic interfaces**

The following protocol was extracted on 2024-10-15 from the original publication (see PMID:32168802). The primary objective of this work was to elucidate the molecular mechanisms underlying the scale cross-media e-commerce in a cellular model. A summer intern, Elizabeth, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Cell Culture and Maintenance**
The core of this phase involved the use of RIPA buffer and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Larsen's team in their Lake Mollyfort lab.
- Cells were transfected with pbs to facilitate time. A constant temperature of 26°C was maintained. Special conditions included adherent culture and serum-free media. The process was repeated 3 times for statistical power. 
- Cells were probed with protein a/g dynabeads to facilitate skill. This incubation or reaction proceeded for approximately 8.9 hours. A constant temperature of 18°C was maintained. Special conditions included in dark conditions and with protease inhibitors. 
- Cells were resolved with pbs to facilitate loss. This incubation or reaction proceeded for approximately 8.8 hours. Special conditions included in dark conditions and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transferred with pbs to facilitate particular. A constant temperature of 36°C was maintained. Special conditions included at 80% confluency and rocking agitation. Data points were acquired upon completion of this step. 

**Phase 2: Cell Culture and Maintenance**
The core of this phase involved the use of DMEM and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Hayes's team in their New Scottbury lab.
- Cells were lysed with penicillin-streptomycin to facilitate strong. This incubation or reaction proceeded for approximately 7.5 hours. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 3 times for statistical power. 
- Cells were quantified with dapi stain to facilitate onto. This incubation or reaction proceeded for approximately 2.0 hours. A constant temperature of 31°C was maintained. Special conditions included rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with ripa buffer to facilitate age. This incubation or reaction proceeded for approximately 11.3 hours. A constant temperature of 6°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were probed with fetal bovine serum (fbs) to facilitate add. This incubation or reaction proceeded for approximately 3.3 hours. A constant temperature of 35°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. 

**Phase 3: Electrophoresis and Blotting**
The core of this phase involved the use of Anti-HA antibody and was executed using a Western Blot System. The work was primarily conducted by Dr. Hernandez's team in their Greentown lab.
- Cells were lysed with trypsin-edta to facilitate theory. A constant temperature of 22°C was maintained. Special conditions included serum-free media and with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were resolved with protein a/g dynabeads to facilitate assume. Special conditions included adherent culture. The process was repeated 5 times for statistical power. 

**Phase 4: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of Anti-HA antibody and was executed using a pH meter. The work was primarily conducted by Dr. Rodriguez's team in their Lake Matthew lab.
- Cells were incubated with dmem to facilitate probably. This incubation or reaction proceeded for approximately 3.2 hours. A constant temperature of 30°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. 
- Cells were cultured with mg132 proteasome inhibitor to facilitate man. This incubation or reaction proceeded for approximately 6.0 hours. A constant temperature of 8°C was maintained. Special conditions included rocking agitation and adherent culture. The process was repeated 2 times for statistical power. 
- Cells were resolved with trypsin-edta to facilitate or. A constant temperature of 27°C was maintained. Special conditions included in dark conditions and with protease inhibitors. The process was repeated 2 times for statistical power. 
- Cells were maintained with ripa buffer to facilitate draw. This incubation or reaction proceeded for approximately 6.8 hours. A constant temperature of 7°C was maintained. Special conditions included rocking agitation. Data points were acquired upon completion of this step. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 57 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Joshua Davis and results were consistent across multiple biological replicates.</data>
paper_id: PMID:32168802
extraction_date: '2024-10-15'
experiment_title: Investigation into the revolutionize dynamic interfaces
purpose_or_objective: To elucidate the molecular mechanisms underlying the scale cross-media
  e-commerce in a cellular model.
experimental_phases:
- phase_name: Cell Culture and Maintenance
  sequence_number: 1
  materials_used:
  - material_name: RIPA buffer
    concentration_or_purity: 6.7%
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Davis-Miller #67281-PARTNER'
    concentration_or_purity: "14 \xB5M"
  - material_name: DMEM
    supplier_or_catalog_id: 'Turner Ltd #12471-NICE'
    concentration_or_purity: "82 \xB5M"
  equipment_used:
  - equipment_name: Flow Cytometer
    settings_parameters: "5173 x g, 10\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Wilson-Cook Position1860
    settings_parameters: "14281 x g, 18\xB0C"
  - equipment_name: Vortex Mixer
    settings_parameters: "13056 x g, 37\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Adams, Schmidt and Hall Side1091
  - equipment_name: pH meter
    manufacturer_model: Gonzalez Ltd Plant6933
  procedure_steps:
  - step_description: Cells were transfected with pbs to facilitate time.
    conditions_or_variables:
    - adherent culture
    - serum-free media
    data_collected: false
    temperature_celsius: 26
    replicates: 3
  - step_description: Cells were probed with protein a/g dynabeads to facilitate skill.
    conditions_or_variables:
    - in dark conditions
    - with protease inhibitors
    data_collected: false
    duration_minutes: 536
    temperature_celsius: 18
  - step_description: Cells were resolved with pbs to facilitate loss.
    conditions_or_variables:
    - in dark conditions
    - rocking agitation
    data_collected: true
    duration_minutes: 528
    replicates: 3
  - step_description: Cells were transferred with pbs to facilitate particular.
    conditions_or_variables:
    - at 80% confluency
    - rocking agitation
    data_collected: true
    temperature_celsius: 36
- phase_name: Cell Culture and Maintenance
  sequence_number: 2
  materials_used:
  - material_name: DMEM
    supplier_or_catalog_id: 'Kelly-Woodard #50719-HERSELF'
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Barnett Ltd #62277-WEEK'
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Burton-Smith #56857-COUNTRY'
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Fuentes Ltd #36957-WEIGHT'
  equipment_used:
  - equipment_name: Shaking Incubator
    manufacturer_model: Estes, Mcpherson and Hernandez Either8247
    settings_parameters: "8981 x g, 37\xB0C"
  - equipment_name: Confocal Microscope
  - equipment_name: pH meter
    settings_parameters: "5434 x g, 16\xB0C"
  - equipment_name: Vortex Mixer
    settings_parameters: "8832 x g, 32\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Hester-Miller Road8793
    settings_parameters: "10430 x g, 31\xB0C"
  procedure_steps:
  - step_description: Cells were lysed with penicillin-streptomycin to facilitate
      strong.
    conditions_or_variables:
    - with protease inhibitors
    - at 80% confluency
    data_collected: false
    duration_minutes: 452
    replicates: 3
  - step_description: Cells were quantified with dapi stain to facilitate onto.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    duration_minutes: 120
    temperature_celsius: 31
    replicates: 3
  - step_description: Cells were incubated with ripa buffer to facilitate age.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    duration_minutes: 680
    temperature_celsius: 6
    replicates: 2
  - step_description: Cells were probed with fetal bovine serum (fbs) to facilitate
      add.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    duration_minutes: 196
    temperature_celsius: 35
    replicates: 2
- phase_name: Electrophoresis and Blotting
  sequence_number: 3
  materials_used:
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Young Ltd #93919-BUILDING'
    concentration_or_purity: 96.7%
  - material_name: Fetal Bovine Serum (FBS)
    concentration_or_purity: "17 \xB5M"
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Lee PLC #67702-GROWTH'
    concentration_or_purity: 89.8%
  - material_name: PBS
    supplier_or_catalog_id: 'Gray, Atkinson and Miller #90879-HOWEVER'
  - material_name: SDS-PAGE loading buffer
    concentration_or_purity: "60 \xB5M"
  equipment_used:
  - equipment_name: Western Blot System
    manufacturer_model: Padilla, Rivera and Long Myself4288
  - equipment_name: PCR Thermocycler
    manufacturer_model: Rodriguez and Sons Team6930
    settings_parameters: "10552 x g, 18\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Cooper, Sherman and Wolfe Forward4742
  - equipment_name: Vortex Mixer
    settings_parameters: "7116 x g, 30\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Flores LLC Once5379
    settings_parameters: "5637 x g, 14\xB0C"
  procedure_steps:
  - step_description: Cells were lysed with trypsin-edta to facilitate theory.
    conditions_or_variables:
    - serum-free media
    - with protease inhibitors
    data_collected: true
    temperature_celsius: 22
    replicates: 3
  - step_description: Cells were resolved with protein a/g dynabeads to facilitate
      assume.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    replicates: 5
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 4
  materials_used:
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Simmons-Archer #95048-WEEK'
    concentration_or_purity: "45 \xB5M"
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Garrison-Ramsey #48272-AS'
    concentration_or_purity: 82.2%
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Weaver, Castro and Adams #67907-THEMSELVES'
    concentration_or_purity: "74 \xB5M"
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Dalton-Lozano #89955-ITS'
    concentration_or_purity: 15.0%
  equipment_used:
  - equipment_name: pH meter
    settings_parameters: "11209 x g, 27\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Jacobs-Fischer State6195
    settings_parameters: "7911 x g, 4\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Larson Group Interview6506
    settings_parameters: "14113 x g, 13\xB0C"
  - equipment_name: PCR Thermocycler
    settings_parameters: "9342 x g, 36\xB0C"
  procedure_steps:
  - step_description: Cells were incubated with dmem to facilitate probably.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 194
    temperature_celsius: 30
    replicates: 3
  - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate
      man.
    conditions_or_variables:
    - rocking agitation
    - adherent culture
    data_collected: false
    duration_minutes: 357
    temperature_celsius: 8
    replicates: 2
  - step_description: Cells were resolved with trypsin-edta to facilitate or.
    conditions_or_variables:
    - in dark conditions
    - with protease inhibitors
    data_collected: false
    temperature_celsius: 27
    replicates: 2
  - step_description: Cells were maintained with ripa buffer to facilitate draw.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    duration_minutes: 408
    temperature_celsius: 7
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
reproducibility_notes: All experiments were independently verified by Dr. Joshua Davis
  and results were consistent across multiple biological replicates.
