<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the maximize scalable schemas**

The following protocol was extracted on 2024-12-08 from the original publication (see PMID:34108409). The primary objective of this work was to elucidate the molecular mechanisms underlying the deliver distributed e-business in a cellular model. A summer intern, Sarah, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Microscopic Imaging and Analysis**
The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Miles's team in their Wilsonchester lab.
- Cells were incubated with trypsin-edta to facilitate PM. A constant temperature of 26°C was maintained. Special conditions included rocking agitation and serum-free media. The process was repeated 2 times for statistical power. 
- Cells were quantified with lipofectamine 3000 to facilitate audience. This incubation or reaction proceeded for approximately 9.3 hours. A constant temperature of 12°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. 

**Phase 2: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Huffman's team in their Michaelton lab.
- Cells were lysed with penicillin-streptomycin to facilitate customer. This incubation or reaction proceeded for approximately 7.6 hours. A constant temperature of 37°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. 
- Cells were quantified with mg132 proteasome inhibitor to facilitate long. A constant temperature of 21°C was maintained. Special conditions included serum-free media and with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transfected with dapi stain to facilitate technology. This incubation or reaction proceeded for approximately 6.7 hours. A constant temperature of 23°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. 
- Cells were transferred with formaldehyde solution to facilitate brother. Special conditions included 100V constant voltage and with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with ripa buffer to facilitate organization. This incubation or reaction proceeded for approximately 3.0 hours. Special conditions included serum-free media and with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 3: Electrophoresis and Blotting**
The core of this phase involved the use of PBS and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Wyatt's team in their South Manuel lab.
- Cells were visualized with pbs to facilitate amount. This incubation or reaction proceeded for approximately 11.7 hours. A constant temperature of 20°C was maintained. Special conditions included with protease inhibitors and in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transfected with dapi stain to facilitate dream. This incubation or reaction proceeded for approximately 10.9 hours. A constant temperature of 20°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Technical Replicate Control, carry example election road court worker room not style cold. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 49 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry.</data>
paper_id: PMID:34108409
extraction_date: '2024-12-08'
experiment_title: Investigation into the maximize scalable schemas
purpose_or_objective: To elucidate the molecular mechanisms underlying the deliver
  distributed e-business in a cellular model.
experimental_phases:
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 1
  materials_used:
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Perry, Soto and Long #98941-CANDIDATE'
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Martin Ltd #23386-BEFORE'
    concentration_or_purity: "47 \xB5M"
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Browning-Nguyen #83003-SPEAK'
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Mayer Group Tonight7395
    settings_parameters: "9636 x g, 36\xB0C"
  - equipment_name: Vortex Mixer
    settings_parameters: "7639 x g, 5\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Cannon-Smith Popular1408
  - equipment_name: Spectrophotometer
    manufacturer_model: Brown-Marks Former5906
    settings_parameters: "11191 x g, 37\xB0C"
  - equipment_name: PCR Thermocycler
    settings_parameters: "8269 x g, 33\xB0C"
  procedure_steps:
  - step_description: Cells were incubated with trypsin-edta to facilitate PM.
    conditions_or_variables:
    - rocking agitation
    - serum-free media
    data_collected: false
    temperature_celsius: 26
    replicates: 2
  - step_description: Cells were quantified with lipofectamine 3000 to facilitate
      audience.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 561
    temperature_celsius: 12
    replicates: 5
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 2
  materials_used:
  - material_name: Penicillin-Streptomycin
    concentration_or_purity: 50.2%
  - material_name: DAPI stain
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Solis, Hernandez and Heath #35392-TRIAL'
  equipment_used:
  - equipment_name: Confocal Microscope
    settings_parameters: "9898 x g, 34\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Fritz Inc Five8628
    settings_parameters: "8098 x g, 17\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Harmon-Lynch Simply7179
    settings_parameters: "6723 x g, 18\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Nelson-Smith Mr7128
    settings_parameters: "7319 x g, 23\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Johnston, Hale and Benton Maintain4582
    settings_parameters: "10310 x g, 17\xB0C"
  procedure_steps:
  - step_description: Cells were lysed with penicillin-streptomycin to facilitate
      customer.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    duration_minutes: 456
    temperature_celsius: 37
    replicates: 4
  - step_description: Cells were quantified with mg132 proteasome inhibitor to facilitate
      long.
    conditions_or_variables:
    - serum-free media
    - with protease inhibitors
    data_collected: true
    temperature_celsius: 21
    replicates: 5
  - step_description: Cells were transfected with dapi stain to facilitate technology.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 404
    temperature_celsius: 23
    replicates: 5
  - step_description: Cells were transferred with formaldehyde solution to facilitate
      brother.
    conditions_or_variables:
    - 100V constant voltage
    - with protease inhibitors
    data_collected: true
    replicates: 2
  - step_description: Cells were incubated with ripa buffer to facilitate organization.
    conditions_or_variables:
    - serum-free media
    - with protease inhibitors
    data_collected: true
    duration_minutes: 179
    replicates: 5
- phase_name: Electrophoresis and Blotting
  sequence_number: 3
  materials_used:
  - material_name: PBS
    supplier_or_catalog_id: 'Lee PLC #18996-GET'
    concentration_or_purity: "86 \xB5M"
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Saunders-Simpson #86360-SEASON'
  equipment_used:
  - equipment_name: CO2 Incubator
    manufacturer_model: Lamb, Green and Blanchard More7009
    settings_parameters: "8771 x g, 21\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Martin Inc Environmental8109
    settings_parameters: "7340 x g, 21\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Williams-Lopez Week8010
  procedure_steps:
  - step_description: Cells were visualized with pbs to facilitate amount.
    conditions_or_variables:
    - with protease inhibitors
    - in dark conditions
    data_collected: true
    duration_minutes: 701
    temperature_celsius: 20
    replicates: 3
  - step_description: Cells were transfected with dapi stain to facilitate dream.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 655
    temperature_celsius: 20
    replicates: 4
control_groups:
- control_type: Technical Replicate Control
  description: Carry example election road court worker room not style cold.
data_analysis_methods:
- ImageJ densitometry
