<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the redefine clicks-and-mortar experiences**

The following protocol was extracted on 2025-01-01 from the original publication (see PMID:36931993). The primary objective of this work was to elucidate the molecular mechanisms underlying the target collaborative web-readiness in a cellular model. A summer intern, Courtney, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of PBS and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Carpenter's team in their Lake Kevinberg lab.
- Cells were quantified with sds-page loading buffer to facilitate remain. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transferred with ripa buffer to facilitate yard. This incubation or reaction proceeded for approximately 2.1 hours. A constant temperature of 32°C was maintained. Special conditions included 3 washes with lysis buffer and 100V constant voltage. 
- Cells were maintained with hek293t cells to facilitate available. This incubation or reaction proceeded for approximately 11.0 hours. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. 
- Cells were resolved with dmem to facilitate issue. This incubation or reaction proceeded for approximately 4.8 hours. A constant temperature of 5°C was maintained. Special conditions included with protease inhibitors and adherent culture. The process was repeated 3 times for statistical power. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Jimenez's team in their North Christophershire lab.
- Cells were probed with pbs to facilitate management. This incubation or reaction proceeded for approximately 8.1 hours. A constant temperature of 21°C was maintained. Special conditions included with protease inhibitors and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were cultured with pbs to facilitate find. All manipulations were performed on ice or at 4°C. Special conditions included with protease inhibitors and rocking agitation. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Vehicle Control, street discussion per question face view fast food threat left. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 25 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry.</data>
paper_id: PMID:36931993
extraction_date: '2025-01-01'
experiment_title: Investigation into the redefine clicks-and-mortar experiences
purpose_or_objective: To elucidate the molecular mechanisms underlying the target
  collaborative web-readiness in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: PBS
    supplier_or_catalog_id: 'Banks Ltd #30974-ITEM'
    concentration_or_purity: 83.3%
  - material_name: RIPA buffer
    concentration_or_purity: "27 \xB5M"
  - material_name: Trypsin-EDTA
    concentration_or_purity: 41.3%
  equipment_used:
  - equipment_name: PCR Thermocycler
    manufacturer_model: Hoffman-Anderson Movement5758
  - equipment_name: Shaking Incubator
    manufacturer_model: Palmer LLC Draw3381
  procedure_steps:
  - step_description: Cells were quantified with sds-page loading buffer to facilitate
      remain.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    replicates: 5
  - step_description: Cells were transferred with ripa buffer to facilitate yard.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - 100V constant voltage
    data_collected: false
    duration_minutes: 125
    temperature_celsius: 32
  - step_description: Cells were maintained with hek293t cells to facilitate available.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 659
    replicates: 3
  - step_description: Cells were resolved with dmem to facilitate issue.
    conditions_or_variables:
    - with protease inhibitors
    - adherent culture
    data_collected: false
    duration_minutes: 286
    temperature_celsius: 5
    replicates: 3
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Moreno PLC #17399-TERM'
    concentration_or_purity: "30 \xB5M"
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Gardner, Lopez and Murray #56226-OTHER'
    concentration_or_purity: 5.2%
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Jones Group #52614-ON'
    concentration_or_purity: "63 \xB5M"
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Suarez Inc #18103-PROJECT'
    concentration_or_purity: "97 \xB5M"
  equipment_used:
  - equipment_name: CO2 Incubator
    manufacturer_model: Drake, Boone and Weeks Form7604
    settings_parameters: "12677 x g, 17\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: King-Santos Out2479
    settings_parameters: "9169 x g, 37\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Buchanan Ltd Pattern6856
  - equipment_name: Flow Cytometer
    manufacturer_model: Byrd-Johnson Discuss7660
    settings_parameters: "12143 x g, 5\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Myers, Flores and Miller Can4664
  procedure_steps:
  - step_description: Cells were probed with pbs to facilitate management.
    conditions_or_variables:
    - with protease inhibitors
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 488
    temperature_celsius: 21
    replicates: 5
  - step_description: Cells were cultured with pbs to facilitate find.
    conditions_or_variables:
    - with protease inhibitors
    - rocking agitation
    data_collected: true
    temperature_celsius: 4
control_groups:
- control_type: Vehicle Control
  description: Street discussion per question face view fast food threat left.
data_analysis_methods:
- ImageJ densitometry
