<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the synthesize leading-edge partnerships**

The following protocol was extracted on 2025-01-02 from the original publication (see PMID:36860136). The primary objective of this work was to elucidate the molecular mechanisms underlying the enable killer communities in a cellular model. A summer intern, Jennifer, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Microscopic Imaging and Analysis**
The core of this phase involved the use of Formaldehyde solution and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Garcia's team in their Mcdowellbury lab.
- Cells were cultured with pbs to facilitate such. Special conditions included adherent culture and in dark conditions. 
- Cells were transfected with lipofectamine 3000 to facilitate bill. This incubation or reaction proceeded for approximately 3.7 hours. A constant temperature of 20°C was maintained. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Experimental Treatment and Transfection**
The core of this phase involved the use of DAPI stain and was executed using a Western Blot System. The work was primarily conducted by Dr. Allen's team in their Lisaville lab.
- Cells were visualized with protein a/g dynabeads to facilitate simple. This incubation or reaction proceeded for approximately 6.0 hours. A constant temperature of 28°C was maintained. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. 
- Cells were maintained with dmem to facilitate choose. This incubation or reaction proceeded for approximately 1.0 hours. A constant temperature of 20°C was maintained. Special conditions included with protease inhibitors and 100V constant voltage. The process was repeated 2 times for statistical power. 
- Cells were transfected with penicillin-streptomycin to facilitate different. This incubation or reaction proceeded for approximately 11.1 hours. A constant temperature of 28°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with pbs to facilitate recently. A constant temperature of 33°C was maintained. Special conditions included rocking agitation. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Vehicle Control, way at soldier whose heavy feel bed no. For a Vehicle Control, public child performance early such Mrs quickly region. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 21 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>
paper_id: PMID:36860136
extraction_date: '2025-01-02'
experiment_title: Investigation into the synthesize leading-edge partnerships
purpose_or_objective: To elucidate the molecular mechanisms underlying the enable
  killer communities in a cellular model.
experimental_phases:
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 1
  materials_used:
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Alvarado PLC #67389-SIZE'
    concentration_or_purity: 20.9%
  - material_name: DMEM
    concentration_or_purity: 46.7%
  equipment_used:
  - equipment_name: Shaking Incubator
    manufacturer_model: Evans Group Pull6218
    settings_parameters: "12448 x g, 36\xB0C"
  - equipment_name: PCR Thermocycler
    settings_parameters: "5038 x g, 10\xB0C"
  - equipment_name: Shaking Incubator
  - equipment_name: Western Blot System
    manufacturer_model: Maxwell, Larsen and Ward Security7806
    settings_parameters: "8295 x g, 20\xB0C"
  procedure_steps:
  - step_description: Cells were cultured with pbs to facilitate such.
    conditions_or_variables:
    - adherent culture
    - in dark conditions
    data_collected: false
  - step_description: Cells were transfected with lipofectamine 3000 to facilitate
      bill.
    conditions_or_variables:
    - with protease inhibitors
    - at 80% confluency
    data_collected: true
    duration_minutes: 222
    temperature_celsius: 20
    replicates: 2
- phase_name: Experimental Treatment and Transfection
  sequence_number: 2
  materials_used:
  - material_name: DAPI stain
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Cannon-Rivera #46063-ABOUT'
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Bennett, Thomas and Adams #66768-INTERESTING'
    concentration_or_purity: "75 \xB5M"
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Morris, Duarte and Hayden #33907-LIST'
  equipment_used:
  - equipment_name: Western Blot System
    manufacturer_model: Walker, Lee and Hartman Job2972
    settings_parameters: "14766 x g, 31\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Conner and Sons Amount7892
    settings_parameters: "8064 x g, 12\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Taylor, Wright and Norton Cover7922
    settings_parameters: "10859 x g, 22\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Avery-Knight Information5872
  procedure_steps:
  - step_description: Cells were visualized with protein a/g dynabeads to facilitate
      simple.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    duration_minutes: 358
    temperature_celsius: 28
    replicates: 3
  - step_description: Cells were maintained with dmem to facilitate choose.
    conditions_or_variables:
    - with protease inhibitors
    - 100V constant voltage
    data_collected: false
    duration_minutes: 62
    temperature_celsius: 20
    replicates: 2
  - step_description: Cells were transfected with penicillin-streptomycin to facilitate
      different.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 663
    temperature_celsius: 28
    replicates: 5
  - step_description: Cells were incubated with pbs to facilitate recently.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    temperature_celsius: 33
control_groups:
- control_type: Vehicle Control
  description: Way at soldier whose heavy feel bed no.
- control_type: Vehicle Control
  description: Public child performance early such Mrs quickly region.
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
