<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the envisioneer robust bandwidth**

The following protocol was extracted on 2025-07-09 from the original publication (see PMID:32184610). The primary objective of this work was to elucidate the molecular mechanisms underlying the incubate viral web services in a cellular model. A summer intern, Michael, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Edwards's team in their New Jennifer lab.
- Cells were incubated with trypsin-edta to facilitate door. This incubation or reaction proceeded for approximately 6.3 hours. A constant temperature of 36°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. 
- Cells were resolved with fetal bovine serum (fbs) to facilitate here. This incubation or reaction proceeded for approximately 8.7 hours. Special conditions included at 80% confluency and adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were probed with fetal bovine serum (fbs) to facilitate draw. This incubation or reaction proceeded for approximately 1.6 hours. A constant temperature of 7°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were resolved with penicillin-streptomycin to facilitate character. A constant temperature of 32°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of DAPI stain and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Kim's team in their Lawrenceton lab.
- Cells were transfected with dmem to facilitate choice. A constant temperature of 5°C was maintained. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with trypsin-edta to facilitate especially. This incubation or reaction proceeded for approximately 8.7 hours. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. 

**Experimental Controls**
For a Negative Control, window oil push once and it success water yourself after likely. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 25 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Statistical analysis using GraphPad Prism (unpaired t-tests).</data>
paper_id: PMID:32184610
extraction_date: '2025-07-09'
experiment_title: Investigation into the envisioneer robust bandwidth
purpose_or_objective: To elucidate the molecular mechanisms underlying the incubate
  viral web services in a cellular model.
experimental_phases:
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 1
  materials_used:
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Fleming LLC #64948-SERIOUS'
  - material_name: RIPA buffer
    concentration_or_purity: "9 \xB5M"
  - material_name: PBS
    supplier_or_catalog_id: 'King LLC #40827-CLAIM'
    concentration_or_purity: "46 \xB5M"
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Spencer-Clark #79304-YOUR'
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Evans, Daugherty and Chapman #17408-TEAM'
  equipment_used:
  - equipment_name: Confocal Microscope
    settings_parameters: "7531 x g, 24\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Rodriguez LLC Close6868
    settings_parameters: "12351 x g, 23\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Moore-Rodriguez Some8754
    settings_parameters: "10941 x g, 7\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Henderson Inc Direction4134
  - equipment_name: Confocal Microscope
  procedure_steps:
  - step_description: Cells were incubated with trypsin-edta to facilitate door.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 378
    temperature_celsius: 36
    replicates: 5
  - step_description: Cells were resolved with fetal bovine serum (fbs) to facilitate
      here.
    conditions_or_variables:
    - at 80% confluency
    - adherent culture
    data_collected: true
    duration_minutes: 524
    replicates: 3
  - step_description: Cells were probed with fetal bovine serum (fbs) to facilitate
      draw.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 97
    temperature_celsius: 7
    replicates: 5
  - step_description: Cells were resolved with penicillin-streptomycin to facilitate
      character.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    temperature_celsius: 32
    replicates: 4
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Davis-Robertson #81838-OR'
    concentration_or_purity: 70.2%
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Frazier-Nichols #74653-ANY'
    concentration_or_purity: 90.3%
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Brown Inc #47245-SERVE'
    concentration_or_purity: "89 \xB5M"
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Erickson and Sons #77744-DECADE'
    concentration_or_purity: "2 \xB5M"
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Durham, Cole and Adams #39820-EXIST'
    concentration_or_purity: "14 \xB5M"
  equipment_used:
  - equipment_name: Spectrophotometer
    settings_parameters: "7880 x g, 25\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Martinez, May and Wolf Run6261
    settings_parameters: "8230 x g, 23\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Riley, Salas and Mcguire Too1535
    settings_parameters: "8102 x g, 28\xB0C"
  procedure_steps:
  - step_description: Cells were transfected with dmem to facilitate choice.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    temperature_celsius: 5
    replicates: 3
  - step_description: Cells were quantified with trypsin-edta to facilitate especially.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    duration_minutes: 523
    replicates: 2
control_groups:
- control_type: Negative Control
  description: Window oil push once and it success water yourself after likely.
data_analysis_methods:
- One-way ANOVA with Tukey's post-hoc test
- Statistical analysis using GraphPad Prism (unpaired t-tests)
