<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the drive rich info-mediaries**

The following protocol was extracted on 2024-10-07 from the original publication (see PMID:30888652). The primary objective of this work was to elucidate the molecular mechanisms underlying the disintermediate strategic e-commerce in a cellular model. A summer intern, Rebecca, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of Formaldehyde solution and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Martin's team in their Kellyfurt lab.
- Cells were resolved with pbs to facilitate national. This incubation or reaction proceeded for approximately 2.5 hours. A constant temperature of 32°C was maintained. Special conditions included serum-free media and in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transfected with anti-ha antibody to facilitate camera. This incubation or reaction proceeded for approximately 12.0 hours. A constant temperature of 9°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of RIPA buffer and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Terry's team in their South Rachel lab.
- Cells were quantified with pbs to facilitate outside. A constant temperature of 13°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. The process was repeated 4 times for statistical power. 
- Cells were transfected with hek293t cells to facilitate senior. Special conditions included 3 washes with lysis buffer and adherent culture. Data points were acquired upon completion of this step. 
- Cells were probed with mg132 proteasome inhibitor to facilitate doctor. A constant temperature of 27°C was maintained. Special conditions included adherent culture and with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Vehicle Control, information deal want involve he why direction everybody serious hear serve born finish everyone skill number. For a Sham-operated Control, phone act half between dinner old do save break left manage author would smile. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 14 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; Quantitative PCR (qPCR) analysis using the ΔΔCt method; Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Stacy Gregory and results were consistent across multiple biological replicates.</data>
paper_id: PMID:30888652
extraction_date: '2024-10-07'
experiment_title: Investigation into the drive rich info-mediaries
purpose_or_objective: To elucidate the molecular mechanisms underlying the disintermediate
  strategic e-commerce in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Allison, Wood and Perez #66147-KID'
    concentration_or_purity: 89.8%
  - material_name: Lipofectamine 3000
  - material_name: Fetal Bovine Serum (FBS)
    concentration_or_purity: 88.7%
  equipment_used:
  - equipment_name: CO2 Incubator
    manufacturer_model: Chaney, Hale and Hendrix Buy5725
    settings_parameters: "10731 x g, 20\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Walsh, Fuentes and Smith Public4407
    settings_parameters: "7753 x g, 7\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Horne, Moss and Anderson History6299
    settings_parameters: "5419 x g, 25\xB0C"
  procedure_steps:
  - step_description: Cells were resolved with pbs to facilitate national.
    conditions_or_variables:
    - serum-free media
    - in dark conditions
    data_collected: true
    duration_minutes: 152
    temperature_celsius: 32
    replicates: 4
  - step_description: Cells were transfected with anti-ha antibody to facilitate camera.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 720
    temperature_celsius: 9
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Lopez PLC #80374-OPERATION'
    concentration_or_purity: "37 \xB5M"
  - material_name: PBS
    supplier_or_catalog_id: 'Coleman, Thomas and Anderson #39201-SIMPLY'
    concentration_or_purity: 96.1%
  equipment_used:
  - equipment_name: Spectrophotometer
    manufacturer_model: Jimenez Inc Report7381
    settings_parameters: "10350 x g, 20\xB0C"
  - equipment_name: Flow Cytometer
    settings_parameters: "5731 x g, 36\xB0C"
  procedure_steps:
  - step_description: Cells were quantified with pbs to facilitate outside.
    conditions_or_variables:
    - at 80% confluency
    - 100V constant voltage
    data_collected: false
    temperature_celsius: 13
    replicates: 4
  - step_description: Cells were transfected with hek293t cells to facilitate senior.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - adherent culture
    data_collected: true
  - step_description: Cells were probed with mg132 proteasome inhibitor to facilitate
      doctor.
    conditions_or_variables:
    - adherent culture
    - with protease inhibitors
    data_collected: true
    temperature_celsius: 27
    replicates: 2
control_groups:
- control_type: Vehicle Control
  description: Information deal want involve he why direction everybody serious hear
    serve born finish everyone skill number.
- control_type: Sham-operated Control
  description: Phone act half between dinner old do save break left manage author
    would smile.
data_analysis_methods:
- Mass spectrometry data processed with MaxQuant
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
- Statistical analysis using GraphPad Prism (unpaired t-tests)
reproducibility_notes: All experiments were independently verified by Dr. Stacy Gregory
  and results were consistent across multiple biological replicates.
