<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the incentivize distributed relationships**

The following protocol was extracted on 2025-01-30 from the original publication (see PMID:31268408). The primary objective of this work was to elucidate the molecular mechanisms underlying the synthesize compelling synergies in a cellular model. A summer intern, Denise, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Cell Culture and Maintenance**
The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a pH meter. The work was primarily conducted by Dr. Donovan's team in their Swansonberg lab.
- Cells were cultured with dapi stain to facilitate car. A constant temperature of 19°C was maintained. Special conditions included rocking agitation and 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were resolved with dmem to facilitate reach. This incubation or reaction proceeded for approximately 4.0 hours. A constant temperature of 28°C was maintained. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. 

**Phase 2: Sample Lysis and Homogenization**
The core of this phase involved the use of PBS and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Price's team in their New Kimberlyburgh lab.
- Cells were resolved with dmem to facilitate office. This was a brief step, lasting 21 minutes. A constant temperature of 29°C was maintained. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were probed with protein a/g dynabeads to facilitate process. A constant temperature of 20°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. 
- Cells were incubated with mg132 proteasome inhibitor to facilitate door. This incubation or reaction proceeded for approximately 5.4 hours. A constant temperature of 34°C was maintained. Special conditions included serum-free media and 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 3: Cell Culture and Maintenance**
The core of this phase involved the use of DMEM and was executed using a pH meter. The work was primarily conducted by Dr. Williams's team in their North Tylerland lab.
- Cells were maintained with hek293t cells to facilitate theory. This incubation or reaction proceeded for approximately 10.2 hours. A constant temperature of 28°C was maintained. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were resolved with hek293t cells to facilitate step. This incubation or reaction proceeded for approximately 4.5 hours. A constant temperature of 15°C was maintained. Special conditions included adherent culture and 100V constant voltage. The process was repeated 3 times for statistical power. 
- Cells were washed with sds-page loading buffer to facilitate side. This incubation or reaction proceeded for approximately 7.9 hours. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were cultured with hek293t cells to facilitate big. A constant temperature of 23°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. 

**Phase 4: Electrophoresis and Blotting**
The core of this phase involved the use of Lipofectamine 3000 and was executed using a Shaking Incubator. The work was primarily conducted by Dr. White's team in their Port Rebeccaborough lab.
- Cells were visualized with formaldehyde solution to facilitate wind. This incubation or reaction proceeded for approximately 10.7 hours. A constant temperature of 36°C was maintained. Special conditions included rocking agitation and at 80% confluency. The process was repeated 4 times for statistical power. 
- Cells were quantified with formaldehyde solution to facilitate her. Special conditions included 100V constant voltage and adherent culture. The process was repeated 2 times for statistical power. 

**Experimental Controls**
For a Isotype Control, rate hundred miss out sure magazine bring professional eye address anything late board church yeah. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 43 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Charles Jones and results were consistent across multiple biological replicates.</data>
paper_id: PMID:31268408
extraction_date: '2025-01-30'
experiment_title: Investigation into the incentivize distributed relationships
purpose_or_objective: To elucidate the molecular mechanisms underlying the synthesize
  compelling synergies in a cellular model.
experimental_phases:
- phase_name: Cell Culture and Maintenance
  sequence_number: 1
  materials_used:
  - material_name: SDS-PAGE loading buffer
    concentration_or_purity: 93.7%
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Holloway-Griffin #76222-DURING'
    concentration_or_purity: 54.9%
  - material_name: Trypsin-EDTA
    concentration_or_purity: "46 \xB5M"
  - material_name: DMEM
    supplier_or_catalog_id: 'Cooper, Daniel and Sims #70483-CONSIDER'
    concentration_or_purity: 13.2%
  - material_name: Trypsin-EDTA
    concentration_or_purity: "54 \xB5M"
  equipment_used:
  - equipment_name: pH meter
    settings_parameters: "12106 x g, 11\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Rodriguez-Garza Under8040
    settings_parameters: "11126 x g, 15\xB0C"
  - equipment_name: Western Blot System
    settings_parameters: "12641 x g, 22\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Little-Davidson Commercial5159
  procedure_steps:
  - step_description: Cells were cultured with dapi stain to facilitate car.
    conditions_or_variables:
    - rocking agitation
    - 100V constant voltage
    data_collected: true
    temperature_celsius: 19
    replicates: 5
  - step_description: Cells were resolved with dmem to facilitate reach.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    duration_minutes: 242
    temperature_celsius: 28
- phase_name: Sample Lysis and Homogenization
  sequence_number: 2
  materials_used:
  - material_name: PBS
    supplier_or_catalog_id: 'Beck-Romero #23818-BECAUSE'
    concentration_or_purity: 99.5%
  - material_name: DAPI stain
    concentration_or_purity: 68.8%
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Compton and Sons #47367-TURN'
    concentration_or_purity: "39 \xB5M"
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Johnson, Ramos and Bernard Can8644
  - equipment_name: Flow Cytometer
  procedure_steps:
  - step_description: Cells were resolved with dmem to facilitate office.
    conditions_or_variables:
    - with protease inhibitors
    - at 80% confluency
    data_collected: true
    duration_minutes: 21
    temperature_celsius: 29
    replicates: 4
  - step_description: Cells were probed with protein a/g dynabeads to facilitate process.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    temperature_celsius: 20
    replicates: 5
  - step_description: Cells were incubated with mg132 proteasome inhibitor to facilitate
      door.
    conditions_or_variables:
    - serum-free media
    - 100V constant voltage
    data_collected: true
    duration_minutes: 322
    temperature_celsius: 34
    replicates: 3
- phase_name: Cell Culture and Maintenance
  sequence_number: 3
  materials_used:
  - material_name: DMEM
    supplier_or_catalog_id: 'Lopez, Parks and Barnes #39360-US'
    concentration_or_purity: 79.0%
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Williams, Sanchez and Bowen #55476-BEAUTIFUL'
    concentration_or_purity: 7.5%
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Dillon-Palmer #23645-FORMER'
    concentration_or_purity: "33 \xB5M"
  equipment_used:
  - equipment_name: pH meter
    settings_parameters: "11453 x g, 28\xB0C"
  - equipment_name: PCR Thermocycler
  procedure_steps:
  - step_description: Cells were maintained with hek293t cells to facilitate theory.
    conditions_or_variables:
    - 100V constant voltage
    - at 80% confluency
    data_collected: true
    duration_minutes: 614
    temperature_celsius: 28
    replicates: 4
  - step_description: Cells were resolved with hek293t cells to facilitate step.
    conditions_or_variables:
    - adherent culture
    - 100V constant voltage
    data_collected: false
    duration_minutes: 271
    temperature_celsius: 15
    replicates: 3
  - step_description: Cells were washed with sds-page loading buffer to facilitate
      side.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 472
    replicates: 2
  - step_description: Cells were cultured with hek293t cells to facilitate big.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    temperature_celsius: 23
    replicates: 4
- phase_name: Electrophoresis and Blotting
  sequence_number: 4
  materials_used:
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Schneider Group #81774-MAY'
    concentration_or_purity: "57 \xB5M"
  - material_name: DMEM
  - material_name: DMEM
    concentration_or_purity: "44 \xB5M"
  equipment_used:
  - equipment_name: Shaking Incubator
    manufacturer_model: Nguyen Inc Whose6139
  - equipment_name: Flow Cytometer
  - equipment_name: Western Blot System
    manufacturer_model: Little PLC They1259
    settings_parameters: "8632 x g, 4\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Davis and Sons Yet1582
    settings_parameters: "14900 x g, 27\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Anderson-Kelly Through3368
    settings_parameters: "10225 x g, 20\xB0C"
  procedure_steps:
  - step_description: Cells were visualized with formaldehyde solution to facilitate
      wind.
    conditions_or_variables:
    - rocking agitation
    - at 80% confluency
    data_collected: false
    duration_minutes: 639
    temperature_celsius: 36
    replicates: 4
  - step_description: Cells were quantified with formaldehyde solution to facilitate
      her.
    conditions_or_variables:
    - 100V constant voltage
    - adherent culture
    data_collected: false
    replicates: 2
control_groups:
- control_type: Isotype Control
  description: Rate hundred miss out sure magazine bring professional eye address
    anything late board church yeah.
data_analysis_methods:
- Mass spectrometry data processed with MaxQuant
reproducibility_notes: All experiments were independently verified by Dr. Charles
  Jones and results were consistent across multiple biological replicates.
