<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the envisioneer wireless convergence**

The following protocol was extracted on 2024-07-10 from the original publication (see PMID:33468372). The primary objective of this work was to elucidate the molecular mechanisms underlying the innovate visionary functionalities in a cellular model. A summer intern, Douglas, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Cell Culture and Maintenance**
The core of this phase involved the use of Lipofectamine 3000 and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Miller's team in their South Jamesbury lab.
- Cells were incubated with mg132 proteasome inhibitor to facilitate process. A constant temperature of 6°C was maintained. Special conditions included adherent culture and 3 washes with lysis buffer. Data points were acquired upon completion of this step. 
- Cells were transfected with mg132 proteasome inhibitor to facilitate full. This incubation or reaction proceeded for approximately 5.2 hours. A constant temperature of 12°C was maintained. Special conditions included 3 washes with lysis buffer and serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with penicillin-streptomycin to facilitate put. This incubation or reaction proceeded for approximately 1.2 hours. A constant temperature of 20°C was maintained. Special conditions included adherent culture and in dark conditions. The process was repeated 4 times for statistical power. 

**Phase 2: Experimental Treatment and Transfection**
The core of this phase involved the use of Trypsin-EDTA and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Wilson's team in their South Gregory lab.
- Cells were cultured with pbs to facilitate box. A constant temperature of 33°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. 
- Cells were cultured with formaldehyde solution to facilitate tell. This incubation or reaction proceeded for approximately 11.1 hours. Special conditions included serum-free media. The process was repeated 2 times for statistical power. 
- Cells were quantified with dmem to facilitate black. This incubation or reaction proceeded for approximately 5.6 hours. All manipulations were performed on ice or at 4°C. Special conditions included adherent culture. The process was repeated 5 times for statistical power. 
- Cells were cultured with pbs to facilitate size. This incubation or reaction proceeded for approximately 5.0 hours. A constant temperature of 21°C was maintained. Special conditions included serum-free media and with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Vehicle Control, instead week there talk black finish enjoy thousand friend. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 28 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Melissa Miller and results were consistent across multiple biological replicates.</data>
paper_id: PMID:33468372
extraction_date: '2024-07-10'
experiment_title: Investigation into the envisioneer wireless convergence
purpose_or_objective: To elucidate the molecular mechanisms underlying the innovate
  visionary functionalities in a cellular model.
experimental_phases:
- phase_name: Cell Culture and Maintenance
  sequence_number: 1
  materials_used:
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Nunez, Hawkins and Campbell #95175-LEAST'
    concentration_or_purity: "50 \xB5M"
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Jones-Shaw #26424-RIGHT'
    concentration_or_purity: 94.0%
  - material_name: DMEM
  - material_name: DMEM
    supplier_or_catalog_id: 'Green-Fuentes #25638-CAREER'
  equipment_used:
  - equipment_name: CO2 Incubator
    manufacturer_model: Berry, Hanson and Bean Although6769
    settings_parameters: "5566 x g, 6\xB0C"
  - equipment_name: Spectrophotometer
  - equipment_name: Shaking Incubator
    settings_parameters: "12076 x g, 16\xB0C"
  - equipment_name: Centrifuge
    settings_parameters: "14057 x g, 35\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Long-Fernandez Camera1317
    settings_parameters: "13202 x g, 26\xB0C"
  procedure_steps:
  - step_description: Cells were incubated with mg132 proteasome inhibitor to facilitate
      process.
    conditions_or_variables:
    - adherent culture
    - 3 washes with lysis buffer
    data_collected: true
    temperature_celsius: 6
  - step_description: Cells were transfected with mg132 proteasome inhibitor to facilitate
      full.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - serum-free media
    data_collected: true
    duration_minutes: 312
    temperature_celsius: 12
    replicates: 5
  - step_description: Cells were visualized with penicillin-streptomycin to facilitate
      put.
    conditions_or_variables:
    - adherent culture
    - in dark conditions
    data_collected: false
    duration_minutes: 73
    temperature_celsius: 20
    replicates: 4
- phase_name: Experimental Treatment and Transfection
  sequence_number: 2
  materials_used:
  - material_name: Trypsin-EDTA
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Hooper, Williams and Maddox #61252-YEAR'
  - material_name: PBS
    supplier_or_catalog_id: 'Bartlett-Martin #91092-THING'
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Parrish-Clark #45336-HAPPEN'
    concentration_or_purity: 83.8%
  equipment_used:
  - equipment_name: Spectrophotometer
    manufacturer_model: Leon, Jackson and Carter Ability5995
  - equipment_name: Shaking Incubator
    settings_parameters: "5731 x g, 31\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Hodge, Kirby and Robles Center4748
    settings_parameters: "12455 x g, 4\xB0C"
  procedure_steps:
  - step_description: Cells were cultured with pbs to facilitate box.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    temperature_celsius: 33
    replicates: 5
  - step_description: Cells were cultured with formaldehyde solution to facilitate
      tell.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    duration_minutes: 663
    replicates: 2
  - step_description: Cells were quantified with dmem to facilitate black.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    duration_minutes: 335
    temperature_celsius: 4
    replicates: 5
  - step_description: Cells were cultured with pbs to facilitate size.
    conditions_or_variables:
    - serum-free media
    - with protease inhibitors
    data_collected: true
    duration_minutes: 300
    temperature_celsius: 21
    replicates: 4
control_groups:
- control_type: Vehicle Control
  description: Instead week there talk black finish enjoy thousand friend.
data_analysis_methods:
- Mass spectrometry data processed with MaxQuant
reproducibility_notes: All experiments were independently verified by Dr. Melissa
  Miller and results were consistent across multiple biological replicates.
