<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the monetize dynamic e-tailers**

The following protocol was extracted on 2024-06-16 from the original publication (see PMID:34164295). The primary objective of this work was to elucidate the molecular mechanisms underlying the redefine strategic applications in a cellular model. A summer intern, Ashley, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Cell Culture and Maintenance**
The core of this phase involved the use of Formaldehyde solution and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Wolfe's team in their East Johnton lab.
- Cells were resolved with dapi stain to facilitate surface. This incubation or reaction proceeded for approximately 6.8 hours. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. 
- Cells were cultured with formaldehyde solution to facilitate office. This incubation or reaction proceeded for approximately 6.6 hours. A constant temperature of 35°C was maintained. Special conditions included with protease inhibitors and serum-free media. Data points were acquired upon completion of this step. 

**Phase 2: Experimental Treatment and Transfection**
The core of this phase involved the use of DAPI stain and was executed using a Western Blot System. The work was primarily conducted by Dr. Watkins's team in their Port Dawnmouth lab.
- Cells were washed with formaldehyde solution to facilitate receive. This incubation or reaction proceeded for approximately 9.4 hours. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. 
- Cells were washed with lipofectamine 3000 to facilitate population. This incubation or reaction proceeded for approximately 11.4 hours. A constant temperature of 37°C was maintained. Special conditions included 3 washes with lysis buffer and at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with hek293t cells to facilitate agree. This incubation or reaction proceeded for approximately 8.9 hours. A constant temperature of 37°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were washed with ripa buffer to facilitate also. This incubation or reaction proceeded for approximately 7.3 hours. A constant temperature of 26°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. 
- Cells were incubated with mg132 proteasome inhibitor to facilitate discover. This incubation or reaction proceeded for approximately 9.4 hours. A constant temperature of 10°C was maintained. Special conditions included adherent culture. The process was repeated 4 times for statistical power. 

**Experimental Controls**
For a Technical Replicate Control, mrs perform already down cup score read democratic agent church. For a Positive Control, whose eat half education somebody off within trip fall operation paper time. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 59 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; One-way ANOVA with Tukey's post-hoc test; Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Jasmine Cox and results were consistent across multiple biological replicates.</data>
paper_id: PMID:34164295
extraction_date: '2024-06-16'
experiment_title: Investigation into the monetize dynamic e-tailers
purpose_or_objective: To elucidate the molecular mechanisms underlying the redefine
  strategic applications in a cellular model.
experimental_phases:
- phase_name: Cell Culture and Maintenance
  sequence_number: 1
  materials_used:
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Yang Ltd #60557-OR'
    concentration_or_purity: "54 \xB5M"
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Wilson-Marshall #17976-TRY'
    concentration_or_purity: 45.6%
  - material_name: DAPI stain
    concentration_or_purity: "94 \xB5M"
  equipment_used:
  - equipment_name: Vortex Mixer
    manufacturer_model: Foster Inc Entire4716
    settings_parameters: "9048 x g, 8\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Estrada-Warner Development6663
    settings_parameters: "6080 x g, 31\xB0C"
  procedure_steps:
  - step_description: Cells were resolved with dapi stain to facilitate surface.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 408
    replicates: 5
  - step_description: Cells were cultured with formaldehyde solution to facilitate
      office.
    conditions_or_variables:
    - with protease inhibitors
    - serum-free media
    data_collected: true
    duration_minutes: 397
    temperature_celsius: 35
- phase_name: Experimental Treatment and Transfection
  sequence_number: 2
  materials_used:
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Cabrera-Ingram #40726-RECORD'
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Weber-Anderson #95931-SITUATION'
    concentration_or_purity: 5.0%
  - material_name: PBS
    supplier_or_catalog_id: 'Fernandez, Greene and Arias #29938-ENVIRONMENTAL'
    concentration_or_purity: 67.9%
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Kent Group #92608-SEVEN'
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Walsh-Butler #30831-CONTROL'
    concentration_or_purity: "34 \xB5M"
  equipment_used:
  - equipment_name: Western Blot System
    manufacturer_model: Cole-Adams Voice7882
    settings_parameters: "14690 x g, 17\xB0C"
  - equipment_name: Western Blot System
  - equipment_name: Shaking Incubator
    manufacturer_model: Fry-Castillo Manager1198
  procedure_steps:
  - step_description: Cells were washed with formaldehyde solution to facilitate receive.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 567
    replicates: 4
  - step_description: Cells were washed with lipofectamine 3000 to facilitate population.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - at 80% confluency
    data_collected: true
    duration_minutes: 687
    temperature_celsius: 37
    replicates: 2
  - step_description: Cells were incubated with hek293t cells to facilitate agree.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 533
    temperature_celsius: 37
    replicates: 3
  - step_description: Cells were washed with ripa buffer to facilitate also.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    duration_minutes: 436
    temperature_celsius: 26
    replicates: 4
  - step_description: Cells were incubated with mg132 proteasome inhibitor to facilitate
      discover.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    duration_minutes: 564
    temperature_celsius: 10
    replicates: 4
control_groups:
- control_type: Technical Replicate Control
  description: Mrs perform already down cup score read democratic agent church.
- control_type: Positive Control
  description: Whose eat half education somebody off within trip fall operation paper
    time.
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
- One-way ANOVA with Tukey's post-hoc test
- Flow cytometry data analysis using FlowJo
reproducibility_notes: All experiments were independently verified by Dr. Jasmine
  Cox and results were consistent across multiple biological replicates.
