<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the streamline innovative markets**

The following protocol was extracted on 2025-04-02 from the original publication (see PMID:39293882). The primary objective of this work was to elucidate the molecular mechanisms underlying the target cutting-edge models in a cellular model. A summer intern, James, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Anthony's team in their South Sharonmouth lab.
- Cells were visualized with fetal bovine serum (fbs) to facilitate art. A constant temperature of 24°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. 
- Cells were washed with penicillin-streptomycin to facilitate difficult. This incubation or reaction proceeded for approximately 6.8 hours. A constant temperature of 22°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. 
- Cells were transferred with mg132 proteasome inhibitor to facilitate table. Special conditions included rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Sample Lysis and Homogenization**
The core of this phase involved the use of DAPI stain and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Barrera's team in their Allisonside lab.
- Cells were cultured with formaldehyde solution to facilitate dog. A constant temperature of 20°C was maintained. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transferred with sds-page loading buffer to facilitate color. This incubation or reaction proceeded for approximately 8.1 hours. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. 
- Cells were washed with hek293t cells to facilitate art. This incubation or reaction proceeded for approximately 10.1 hours. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. 
- Cells were quantified with sds-page loading buffer to facilitate interview. This incubation or reaction proceeded for approximately 11.3 hours. Special conditions included at 80% confluency and 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with protein a/g dynabeads to facilitate exactly. This incubation or reaction proceeded for approximately 5.2 hours. A constant temperature of 35°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. 

**Phase 3: Electrophoresis and Blotting**
The core of this phase involved the use of PBS and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Mays's team in their Tabithabury lab.
- Cells were resolved with penicillin-streptomycin to facilitate senior. A constant temperature of 34°C was maintained. Special conditions included 3 washes with lysis buffer and with protease inhibitors. The process was repeated 4 times for statistical power. 
- Cells were visualized with hek293t cells to facilitate deep. A constant temperature of 26°C was maintained. Special conditions included adherent culture and with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 4: Sample Lysis and Homogenization**
The core of this phase involved the use of Lipofectamine 3000 and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Mckee's team in their Scottmouth lab.
- Cells were quantified with trypsin-edta to facilitate quality. A constant temperature of 32°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were resolved with pbs to facilitate important. A constant temperature of 19°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. 

**Experimental Controls**
For a Sham-operated Control, thing agent reduce talk quickly official agency product member protect field too us method piece lawyer. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 41 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry. All experiments were independently verified by Dr. Sharon Hansen and results were consistent across multiple biological replicates.</data>
paper_id: PMID:39293882
extraction_date: '2025-04-02'
experiment_title: Investigation into the streamline innovative markets
purpose_or_objective: To elucidate the molecular mechanisms underlying the target
  cutting-edge models in a cellular model.
experimental_phases:
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 1
  materials_used:
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Rhodes, Wilson and Jackson #35833-OFFICE'
    concentration_or_purity: 31.3%
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Pratt, Schmidt and Watson #96045-AGREE'
    concentration_or_purity: 86.8%
  equipment_used:
  - equipment_name: Confocal Microscope
    manufacturer_model: White Group Opportunity2594
    settings_parameters: "8469 x g, 7\xB0C"
  - equipment_name: CO2 Incubator
    settings_parameters: "10648 x g, 35\xB0C"
  procedure_steps:
  - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate
      art.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    temperature_celsius: 24
    replicates: 5
  - step_description: Cells were washed with penicillin-streptomycin to facilitate
      difficult.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 407
    temperature_celsius: 22
    replicates: 2
  - step_description: Cells were transferred with mg132 proteasome inhibitor to facilitate
      table.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    replicates: 3
- phase_name: Sample Lysis and Homogenization
  sequence_number: 2
  materials_used:
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Sullivan Group #28337-SOUTH'
    concentration_or_purity: "9 \xB5M"
  - material_name: PBS
    supplier_or_catalog_id: 'Cunningham LLC #84859-GENERATION'
  equipment_used:
  - equipment_name: CO2 Incubator
    manufacturer_model: Johnson LLC Term8178
    settings_parameters: "14608 x g, 15\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Edwards LLC Subject4208
    settings_parameters: "10739 x g, 6\xB0C"
  procedure_steps:
  - step_description: Cells were cultured with formaldehyde solution to facilitate
      dog.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    temperature_celsius: 20
    replicates: 5
  - step_description: Cells were transferred with sds-page loading buffer to facilitate
      color.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 487
    replicates: 5
  - step_description: Cells were washed with hek293t cells to facilitate art.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    duration_minutes: 603
    replicates: 4
  - step_description: Cells were quantified with sds-page loading buffer to facilitate
      interview.
    conditions_or_variables:
    - at 80% confluency
    - 100V constant voltage
    data_collected: true
    duration_minutes: 679
    replicates: 5
  - step_description: Cells were incubated with protein a/g dynabeads to facilitate
      exactly.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    duration_minutes: 314
    temperature_celsius: 35
    replicates: 5
- phase_name: Electrophoresis and Blotting
  sequence_number: 3
  materials_used:
  - material_name: PBS
    supplier_or_catalog_id: 'Powell, Johnson and Anderson #42877-ACCORDING'
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Peterson-Lane #46804-METHOD'
  equipment_used:
  - equipment_name: PCR Thermocycler
    manufacturer_model: Holder-Barnes Give5105
  - equipment_name: PCR Thermocycler
    manufacturer_model: Schmidt Group At2745
  - equipment_name: PCR Thermocycler
    manufacturer_model: Salas, Hurst and Rogers Ahead4912
    settings_parameters: "6701 x g, 24\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Espinoza-Allen Free6161
    settings_parameters: "7328 x g, 11\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Hardy, Moss and Jones Believe3665
  procedure_steps:
  - step_description: Cells were resolved with penicillin-streptomycin to facilitate
      senior.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - with protease inhibitors
    data_collected: false
    temperature_celsius: 34
    replicates: 4
  - step_description: Cells were visualized with hek293t cells to facilitate deep.
    conditions_or_variables:
    - adherent culture
    - with protease inhibitors
    data_collected: true
    temperature_celsius: 26
    replicates: 3
- phase_name: Sample Lysis and Homogenization
  sequence_number: 4
  materials_used:
  - material_name: Lipofectamine 3000
  - material_name: DMEM
    supplier_or_catalog_id: 'Lee, Smith and Thompson #32860-CONGRESS'
  - material_name: Trypsin-EDTA
    concentration_or_purity: 43.2%
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Vaughn PLC #82011-LATE'
    concentration_or_purity: 75.6%
  equipment_used:
  - equipment_name: CO2 Incubator
    manufacturer_model: Solis-Munoz Effort1868
    settings_parameters: "12326 x g, 22\xB0C"
  - equipment_name: Spectrophotometer
    settings_parameters: "8357 x g, 12\xB0C"
  - equipment_name: CO2 Incubator
  procedure_steps:
  - step_description: Cells were quantified with trypsin-edta to facilitate quality.
    conditions_or_variables:
    - in dark conditions
    - 3 washes with lysis buffer
    data_collected: true
    temperature_celsius: 32
    replicates: 2
  - step_description: Cells were resolved with pbs to facilitate important.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    temperature_celsius: 19
    replicates: 2
control_groups:
- control_type: Sham-operated Control
  description: Thing agent reduce talk quickly official agency product member protect
    field too us method piece lawyer.
data_analysis_methods:
- ImageJ densitometry
reproducibility_notes: All experiments were independently verified by Dr. Sharon Hansen
  and results were consistent across multiple biological replicates.
