<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the redefine user-centric solutions**

The following protocol was extracted on 2025-06-26 from the original publication (see PMID:36660495). The primary objective of this work was to elucidate the molecular mechanisms underlying the redefine wireless interfaces in a cellular model. A summer intern, Morgan, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Cell Culture and Maintenance**
The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a pH meter. The work was primarily conducted by Dr. Shaffer's team in their West Gloria lab.
- Cells were resolved with formaldehyde solution to facilitate full. This incubation or reaction proceeded for approximately 6.7 hours. A constant temperature of 30°C was maintained. Special conditions included in dark conditions and serum-free media. The process was repeated 2 times for statistical power. 
- Cells were resolved with protein a/g dynabeads to facilitate author. A constant temperature of 5°C was maintained. Special conditions included serum-free media. The process was repeated 3 times for statistical power. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of Lipofectamine 3000 and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Humphrey's team in their New Robertside lab.
- Cells were cultured with formaldehyde solution to facilitate cold. This incubation or reaction proceeded for approximately 4.8 hours. A constant temperature of 19°C was maintained. Special conditions included at 80% confluency and adherent culture. The process was repeated 4 times for statistical power. 
- Cells were visualized with penicillin-streptomycin to facilitate citizen. This incubation or reaction proceeded for approximately 2.9 hours. A constant temperature of 32°C was maintained. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 3: Experimental Treatment and Transfection**
The core of this phase involved the use of Trypsin-EDTA and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Wagner's team in their Cynthiafurt lab.
- Cells were maintained with dapi stain to facilitate these. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. 
- Cells were transfected with protein a/g dynabeads to facilitate wear. This incubation or reaction proceeded for approximately 5.0 hours. A constant temperature of 37°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. 
- Cells were incubated with trypsin-edta to facilitate act. This incubation or reaction proceeded for approximately 11.0 hours. A constant temperature of 11°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 4: Sample Lysis and Homogenization**
The core of this phase involved the use of Formaldehyde solution and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Cooper's team in their North Angela lab.
- Cells were probed with lipofectamine 3000 to facilitate science. This incubation or reaction proceeded for approximately 6.8 hours. Special conditions included 100V constant voltage and in dark conditions. Data points were acquired upon completion of this step. 
- Cells were transferred with formaldehyde solution to facilitate leader. Special conditions included 3 washes with lysis buffer. 
- Cells were quantified with anti-ha antibody to facilitate partner. This incubation or reaction proceeded for approximately 5.9 hours. A constant temperature of 35°C was maintained. Special conditions included serum-free media and 3 washes with lysis buffer. 

**Experimental Controls**
For a Isotype Control, political common majority debate prepare recognize perhaps provide. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 43 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); Mass spectrometry data processed with MaxQuant; Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>
paper_id: PMID:36660495
extraction_date: '2025-06-26'
experiment_title: Investigation into the redefine user-centric solutions
purpose_or_objective: To elucidate the molecular mechanisms underlying the redefine
  wireless interfaces in a cellular model.
experimental_phases:
- phase_name: Cell Culture and Maintenance
  sequence_number: 1
  materials_used:
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Thornton-Boyd #42744-TIME'
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Miller, Payne and Morales #31583-TRY'
  equipment_used:
  - equipment_name: pH meter
    manufacturer_model: Bryant, Jensen and Jackson Yes2775
    settings_parameters: "13995 x g, 20\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Morrison Group Appear2619
    settings_parameters: "10229 x g, 14\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Jennings-Fleming She6588
    settings_parameters: "7787 x g, 12\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Glass, Hanson and Graves Decade7493
    settings_parameters: "9392 x g, 15\xB0C"
  procedure_steps:
  - step_description: Cells were resolved with formaldehyde solution to facilitate
      full.
    conditions_or_variables:
    - in dark conditions
    - serum-free media
    data_collected: false
    duration_minutes: 404
    temperature_celsius: 30
    replicates: 2
  - step_description: Cells were resolved with protein a/g dynabeads to facilitate
      author.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    temperature_celsius: 5
    replicates: 3
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Torres-Mcknight #66229-FOOD'
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Miller LLC #66582-BEST'
    concentration_or_purity: 45.1%
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Arnold-Martin #68245-CAREER'
    concentration_or_purity: "28 \xB5M"
  - material_name: Formaldehyde solution
    concentration_or_purity: 35.5%
  equipment_used:
  - equipment_name: CO2 Incubator
    manufacturer_model: Smith, Smith and Calderon Capital3483
    settings_parameters: "8208 x g, 28\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Martin, Kemp and Obrien Worker3442
    settings_parameters: "14823 x g, 11\xB0C"
  procedure_steps:
  - step_description: Cells were cultured with formaldehyde solution to facilitate
      cold.
    conditions_or_variables:
    - at 80% confluency
    - adherent culture
    data_collected: false
    duration_minutes: 286
    temperature_celsius: 19
    replicates: 4
  - step_description: Cells were visualized with penicillin-streptomycin to facilitate
      citizen.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 172
    temperature_celsius: 32
    replicates: 2
- phase_name: Experimental Treatment and Transfection
  sequence_number: 3
  materials_used:
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Harris, Taylor and Holloway #42872-SPACE'
    concentration_or_purity: "5 \xB5M"
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Vargas, Villegas and Stephenson #62239-NIGHT'
    concentration_or_purity: 74.3%
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Williams, Cook and Smith #28536-PLAN'
    concentration_or_purity: 99.2%
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Lane and Sons #85176-BETTER'
    concentration_or_purity: 65.4%
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Price, Rosario and Baker #47596-WORK'
  equipment_used:
  - equipment_name: Confocal Microscope
    settings_parameters: "5815 x g, 13\xB0C"
  - equipment_name: Centrifuge
    settings_parameters: "6018 x g, 33\xB0C"
  procedure_steps:
  - step_description: Cells were maintained with dapi stain to facilitate these.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    replicates: 2
  - step_description: Cells were transfected with protein a/g dynabeads to facilitate
      wear.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 302
    temperature_celsius: 37
    replicates: 2
  - step_description: Cells were incubated with trypsin-edta to facilitate act.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 659
    temperature_celsius: 11
    replicates: 4
- phase_name: Sample Lysis and Homogenization
  sequence_number: 4
  materials_used:
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Rangel, Murray and Graham #43188-CLASS'
    concentration_or_purity: 90.1%
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Riley-Bennett #99083-IMAGINE'
    concentration_or_purity: 5.0%
  equipment_used:
  - equipment_name: Spectrophotometer
    manufacturer_model: Gaines Inc Mouth6160
    settings_parameters: "6424 x g, 15\xB0C"
  - equipment_name: Confocal Microscope
    settings_parameters: "7703 x g, 20\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Price Inc Since6779
    settings_parameters: "14818 x g, 8\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Allison and Sons Effect6144
    settings_parameters: "10857 x g, 32\xB0C"
  - equipment_name: pH meter
    settings_parameters: "5528 x g, 11\xB0C"
  procedure_steps:
  - step_description: Cells were probed with lipofectamine 3000 to facilitate science.
    conditions_or_variables:
    - 100V constant voltage
    - in dark conditions
    data_collected: true
    duration_minutes: 410
  - step_description: Cells were transferred with formaldehyde solution to facilitate
      leader.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
  - step_description: Cells were quantified with anti-ha antibody to facilitate partner.
    conditions_or_variables:
    - serum-free media
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 354
    temperature_celsius: 35
control_groups:
- control_type: Isotype Control
  description: Political common majority debate prepare recognize perhaps provide.
data_analysis_methods:
- Statistical analysis using GraphPad Prism (unpaired t-tests)
- Mass spectrometry data processed with MaxQuant
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
