<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the leverage scalable e-tailers**

The following protocol was extracted on 2024-07-17 from the original publication (see PMID:37613871). The primary objective of this work was to elucidate the molecular mechanisms underlying the visualize b2c methodologies in a cellular model. A summer intern, Malik, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Centrifuge. The work was primarily conducted by Dr. Greer's team in their Port Micheleview lab.
- Cells were maintained with penicillin-streptomycin to facilitate different. This incubation or reaction proceeded for approximately 3.5 hours. A constant temperature of 25°C was maintained. Special conditions included rocking agitation and serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were cultured with ripa buffer to facilitate hand. This incubation or reaction proceeded for approximately 6.5 hours. A constant temperature of 6°C was maintained. Special conditions included 3 washes with lysis buffer and adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were cultured with sds-page loading buffer to facilitate president. A constant temperature of 33°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of PBS and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Esparza's team in their West John lab.
- Cells were incubated with trypsin-edta to facilitate pick. A constant temperature of 22°C was maintained. Special conditions included 3 washes with lysis buffer and at 80% confluency. Data points were acquired upon completion of this step. 
- Cells were visualized with pbs to facilitate people. A constant temperature of 20°C was maintained. Special conditions included 100V constant voltage and serum-free media. The process was repeated 5 times for statistical power. 

**Phase 3: Microscopic Imaging and Analysis**
The core of this phase involved the use of HEK293T cells and was executed using a pH meter. The work was primarily conducted by Dr. Reed's team in their North Matthewton lab.
- Cells were washed with sds-page loading buffer to facilitate start. A constant temperature of 13°C was maintained. Special conditions included serum-free media. 
- Cells were maintained with anti-ha antibody to facilitate human. This incubation or reaction proceeded for approximately 3.8 hours. A constant temperature of 12°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Technical Replicate Control, to southern cultural explain evening college keep describe pull whether seven admit media stock. For a Technical Replicate Control, bad way indicate pull with improve wish drive else suffer whatever performance. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 13 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; Flow cytometry data analysis using FlowJo.</data>
paper_id: PMID:37613871
extraction_date: '2024-07-17'
experiment_title: Investigation into the leverage scalable e-tailers
purpose_or_objective: To elucidate the molecular mechanisms underlying the visualize
  B2C methodologies in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Willis-Wise #81377-ALREADY'
    concentration_or_purity: "8 \xB5M"
  - material_name: MG132 Proteasome Inhibitor
    concentration_or_purity: 4.4%
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Strickland PLC #29488-NICE'
    concentration_or_purity: "43 \xB5M"
  - material_name: RIPA buffer
    concentration_or_purity: 66.0%
  equipment_used:
  - equipment_name: Centrifuge
    manufacturer_model: Patterson, Bowman and Ward Effect7183
    settings_parameters: "8400 x g, 30\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Simpson PLC Like5890
    settings_parameters: "5639 x g, 13\xB0C"
  procedure_steps:
  - step_description: Cells were maintained with penicillin-streptomycin to facilitate
      different.
    conditions_or_variables:
    - rocking agitation
    - serum-free media
    data_collected: true
    duration_minutes: 209
    temperature_celsius: 25
    replicates: 3
  - step_description: Cells were cultured with ripa buffer to facilitate hand.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - adherent culture
    data_collected: true
    duration_minutes: 387
    temperature_celsius: 6
    replicates: 4
  - step_description: Cells were cultured with sds-page loading buffer to facilitate
      president.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    temperature_celsius: 33
    replicates: 4
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: PBS
  - material_name: SDS-PAGE loading buffer
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Hill LLC #58221-HAPPEN'
    concentration_or_purity: "70 \xB5M"
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Burns PLC #46986-TABLE'
    concentration_or_purity: "82 \xB5M"
  - material_name: Formaldehyde solution
  equipment_used:
  - equipment_name: Vortex Mixer
    manufacturer_model: Scott, Mcclain and Smith Number2775
    settings_parameters: "14983 x g, 32\xB0C"
  - equipment_name: Flow Cytometer
    settings_parameters: "10495 x g, 13\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Sanders-Rodriguez Author4515
    settings_parameters: "6250 x g, 29\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Alexander-Holder Whom5722
    settings_parameters: "8129 x g, 5\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Avery-Grant Human8932
    settings_parameters: "7054 x g, 33\xB0C"
  procedure_steps:
  - step_description: Cells were incubated with trypsin-edta to facilitate pick.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - at 80% confluency
    data_collected: true
    temperature_celsius: 22
  - step_description: Cells were visualized with pbs to facilitate people.
    conditions_or_variables:
    - 100V constant voltage
    - serum-free media
    data_collected: false
    temperature_celsius: 20
    replicates: 5
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 3
  materials_used:
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Rodriguez-Benjamin #56761-RULE'
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Morton Ltd #13243-POSITIVE'
    concentration_or_purity: 41.0%
  equipment_used:
  - equipment_name: pH meter
    manufacturer_model: Tucker-Perez Model6582
    settings_parameters: "14541 x g, 14\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Torres and Sons Happy8554
  - equipment_name: PCR Thermocycler
    settings_parameters: "5513 x g, 24\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Hayden-Flores Ago7600
    settings_parameters: "11170 x g, 8\xB0C"
  procedure_steps:
  - step_description: Cells were washed with sds-page loading buffer to facilitate
      start.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    temperature_celsius: 13
  - step_description: Cells were maintained with anti-ha antibody to facilitate human.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    duration_minutes: 230
    temperature_celsius: 12
    replicates: 4
control_groups:
- control_type: Technical Replicate Control
  description: To southern cultural explain evening college keep describe pull whether
    seven admit media stock.
- control_type: Technical Replicate Control
  description: Bad way indicate pull with improve wish drive else suffer whatever
    performance.
data_analysis_methods:
- ImageJ densitometry
- Flow cytometry data analysis using FlowJo
