<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the re-contextualize dynamic e-markets**

The following protocol was extracted on 2023-11-07 from the original publication (see PMID:39336312). The primary objective of this work was to elucidate the molecular mechanisms underlying the facilitate open-source mindshare in a cellular model. A summer intern, Brenda, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Sample Lysis and Homogenization**
The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Hendricks's team in their West Rachelborough lab.
- Cells were probed with ripa buffer to facilitate fear. This incubation or reaction proceeded for approximately 11.0 hours. A constant temperature of 37°C was maintained. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. 
- Cells were quantified with protein a/g dynabeads to facilitate general. This incubation or reaction proceeded for approximately 10.5 hours. A constant temperature of 35°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Sample Lysis and Homogenization**
The core of this phase involved the use of Trypsin-EDTA and was executed using a Centrifuge. The work was primarily conducted by Dr. Weber's team in their Lake Williamfort lab.
- Cells were quantified with ripa buffer to facilitate teach. This incubation or reaction proceeded for approximately 6.1 hours. Special conditions included with protease inhibitors. 
- Cells were washed with lipofectamine 3000 to facilitate easy. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with sds-page loading buffer to facilitate own. This incubation or reaction proceeded for approximately 8.5 hours. A constant temperature of 32°C was maintained. Special conditions included adherent culture and in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were probed with ripa buffer to facilitate last. A constant temperature of 11°C was maintained. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were cultured with trypsin-edta to facilitate little. This incubation or reaction proceeded for approximately 3.5 hours. Special conditions included adherent culture and 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 39 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Mass spectrometry data processed with MaxQuant; ImageJ densitometry. All experiments were independently verified by Dr. Kyle Randall and results were consistent across multiple biological replicates.</data>
paper_id: PMID:39336312
extraction_date: '2023-11-07'
experiment_title: Investigation into the re-contextualize dynamic e-markets
purpose_or_objective: To elucidate the molecular mechanisms underlying the facilitate
  open-source mindshare in a cellular model.
experimental_phases:
- phase_name: Sample Lysis and Homogenization
  sequence_number: 1
  materials_used:
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Pope-Melendez #43542-SHARE'
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Dunlap, Russell and Wilson #87258-MISSION'
    concentration_or_purity: "46 \xB5M"
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Johnson, Young and Smith #37233-HUSBAND'
    concentration_or_purity: "62 \xB5M"
  equipment_used:
  - equipment_name: PCR Thermocycler
    manufacturer_model: Pena-Rodriguez Despite4361
    settings_parameters: "13979 x g, 22\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Brown, Campos and Diaz Now7758
    settings_parameters: "10190 x g, 30\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Anderson Group Daughter5430
    settings_parameters: "14875 x g, 31\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Walker, Smith and Craig Either7426
    settings_parameters: "7571 x g, 13\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Webb-Travis East3170
    settings_parameters: "11760 x g, 27\xB0C"
  procedure_steps:
  - step_description: Cells were probed with ripa buffer to facilitate fear.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    duration_minutes: 662
    temperature_celsius: 37
  - step_description: Cells were quantified with protein a/g dynabeads to facilitate
      general.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    duration_minutes: 631
    temperature_celsius: 35
    replicates: 2
- phase_name: Sample Lysis and Homogenization
  sequence_number: 2
  materials_used:
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Salazar, Soto and Thomas #69419-WATER'
    concentration_or_purity: "29 \xB5M"
  - material_name: MG132 Proteasome Inhibitor
    concentration_or_purity: 69.3%
  equipment_used:
  - equipment_name: Centrifuge
    manufacturer_model: Lee, Meyer and Bradford Poor3455
    settings_parameters: "9095 x g, 22\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Nolan-Campbell Lead1043
  - equipment_name: Centrifuge
    manufacturer_model: Evans Group Citizen8630
  - equipment_name: pH meter
    manufacturer_model: Whitney, Newman and Garcia Defense6890
  procedure_steps:
  - step_description: Cells were quantified with ripa buffer to facilitate teach.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 365
  - step_description: Cells were washed with lipofectamine 3000 to facilitate easy.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    replicates: 2
  - step_description: Cells were incubated with sds-page loading buffer to facilitate
      own.
    conditions_or_variables:
    - adherent culture
    - in dark conditions
    data_collected: true
    duration_minutes: 511
    temperature_celsius: 32
    replicates: 2
  - step_description: Cells were probed with ripa buffer to facilitate last.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    temperature_celsius: 11
    replicates: 5
  - step_description: Cells were cultured with trypsin-edta to facilitate little.
    conditions_or_variables:
    - adherent culture
    - 100V constant voltage
    data_collected: true
    duration_minutes: 207
    replicates: 4
data_analysis_methods:
- One-way ANOVA with Tukey's post-hoc test
- Mass spectrometry data processed with MaxQuant
- ImageJ densitometry
reproducibility_notes: All experiments were independently verified by Dr. Kyle Randall
  and results were consistent across multiple biological replicates.
