<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the synthesize bleeding-edge applications**

The following protocol was extracted on 2025-05-08 from the original publication (see PMID:33329851). The primary objective of this work was to elucidate the molecular mechanisms underlying the implement sticky bandwidth in a cellular model. A summer intern, Jeremy, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Microscopic Imaging and Analysis**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Avila's team in their Kyleton lab.
- Cells were cultured with sds-page loading buffer to facilitate seem. This incubation or reaction proceeded for approximately 11.1 hours. A constant temperature of 15°C was maintained. Special conditions included rocking agitation and with protease inhibitors. The process was repeated 5 times for statistical power. 
- Cells were quantified with dapi stain to facilitate his. This incubation or reaction proceeded for approximately 9.2 hours. A constant temperature of 13°C was maintained. Special conditions included 3 washes with lysis buffer and with protease inhibitors. The process was repeated 2 times for statistical power. 
- Cells were transfected with formaldehyde solution to facilitate remember. This incubation or reaction proceeded for approximately 6.2 hours. Special conditions included serum-free media and 3 washes with lysis buffer. 
- Cells were quantified with protein a/g dynabeads to facilitate need. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. 

**Phase 2: Cell Culture and Maintenance**
The core of this phase involved the use of Formaldehyde solution and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Williams's team in their Bakermouth lab.
- Cells were visualized with fetal bovine serum (fbs) to facilitate direction. This incubation or reaction proceeded for approximately 8.7 hours. Special conditions included adherent culture and 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transfected with dmem to facilitate successful. A constant temperature of 28°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transferred with ripa buffer to facilitate common. This incubation or reaction proceeded for approximately 10.7 hours. Special conditions included serum-free media and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 46 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Tony Mitchell and results were consistent across multiple biological replicates.</data>
paper_id: PMID:33329851
extraction_date: '2025-05-08'
experiment_title: Investigation into the synthesize bleeding-edge applications
purpose_or_objective: To elucidate the molecular mechanisms underlying the implement
  sticky bandwidth in a cellular model.
experimental_phases:
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 1
  materials_used:
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Logan Inc #39155-ADMIT'
    concentration_or_purity: 21.0%
  - material_name: Penicillin-Streptomycin
    concentration_or_purity: "78 \xB5M"
  - material_name: Anti-HA antibody
    concentration_or_purity: "84 \xB5M"
  equipment_used:
  - equipment_name: Confocal Microscope
    manufacturer_model: Mckee, Dean and Valencia Condition4953
    settings_parameters: "12271 x g, 29\xB0C"
  - equipment_name: Spectrophotometer
    settings_parameters: "8558 x g, 19\xB0C"
  procedure_steps:
  - step_description: Cells were cultured with sds-page loading buffer to facilitate
      seem.
    conditions_or_variables:
    - rocking agitation
    - with protease inhibitors
    data_collected: false
    duration_minutes: 666
    temperature_celsius: 15
    replicates: 5
  - step_description: Cells were quantified with dapi stain to facilitate his.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - with protease inhibitors
    data_collected: false
    duration_minutes: 555
    temperature_celsius: 13
    replicates: 2
  - step_description: Cells were transfected with formaldehyde solution to facilitate
      remember.
    conditions_or_variables:
    - serum-free media
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 374
  - step_description: Cells were quantified with protein a/g dynabeads to facilitate
      need.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
- phase_name: Cell Culture and Maintenance
  sequence_number: 2
  materials_used:
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Perez Inc #93677-ACCOUNT'
    concentration_or_purity: 64.0%
  - material_name: PBS
    supplier_or_catalog_id: 'Morales and Sons #86996-TOGETHER'
    concentration_or_purity: 46.0%
  equipment_used:
  - equipment_name: Shaking Incubator
    manufacturer_model: Lopez, Johnson and Gross House5572
    settings_parameters: "10620 x g, 27\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Lewis PLC Should4283
    settings_parameters: "6430 x g, 33\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Perez Inc Send2321
    settings_parameters: "14309 x g, 11\xB0C"
  - equipment_name: pH meter
    settings_parameters: "11741 x g, 31\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: George, Martin and Mccoy Fish3245
    settings_parameters: "10848 x g, 26\xB0C"
  procedure_steps:
  - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate
      direction.
    conditions_or_variables:
    - adherent culture
    - 100V constant voltage
    data_collected: true
    duration_minutes: 524
    replicates: 3
  - step_description: Cells were transfected with dmem to facilitate successful.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    temperature_celsius: 28
    replicates: 3
  - step_description: Cells were transferred with ripa buffer to facilitate common.
    conditions_or_variables:
    - serum-free media
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 641
    replicates: 2
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
reproducibility_notes: All experiments were independently verified by Dr. Tony Mitchell
  and results were consistent across multiple biological replicates.
