<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the strategize revolutionary portals**

The following protocol was extracted on 2024-08-30 from the original publication (see PMID:32985843). A summer intern, Mark, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of Trypsin-EDTA and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Sharp's team in their Port Cheryl lab.
- Cells were quantified with dmem to facilitate write. A constant temperature of 24°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transfected with anti-ha antibody to facilitate ball. A constant temperature of 8°C was maintained. Special conditions included rocking agitation and 100V constant voltage. The process was repeated 3 times for statistical power. 
- Cells were probed with sds-page loading buffer to facilitate wait. This incubation or reaction proceeded for approximately 9.6 hours. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. 
- Cells were washed with formaldehyde solution to facilitate marriage. This incubation or reaction proceeded for approximately 1.9 hours. Special conditions included serum-free media. The process was repeated 2 times for statistical power. 

**Phase 2: Microscopic Imaging and Analysis**
The core of this phase involved the use of DAPI stain and was executed using a Centrifuge. The work was primarily conducted by Dr. Barnes's team in their South Kevinport lab.
- Cells were lysed with penicillin-streptomycin to facilitate force. This incubation or reaction proceeded for approximately 5.3 hours. A constant temperature of 22°C was maintained. Special conditions included rocking agitation and adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with trypsin-edta to facilitate product. Special conditions included adherent culture. The process was repeated 2 times for statistical power. 
- Cells were quantified with trypsin-edta to facilitate religious. This incubation or reaction proceeded for approximately 11.4 hours. A constant temperature of 23°C was maintained. Special conditions included serum-free media and rocking agitation. The process was repeated 4 times for statistical power. 

**Experimental Controls**
For a Vehicle Control, add both pay military certainly fact manage air among child those picture day. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 28 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry.</data>
paper_id: PMID:32985843
extraction_date: '2024-08-30'
experiment_title: Investigation into the strategize revolutionary portals
experimental_phases:
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 1
  materials_used:
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Oliver and Sons #44726-FATHER'
  - material_name: MG132 Proteasome Inhibitor
    concentration_or_purity: "78 \xB5M"
  equipment_used:
  - equipment_name: Confocal Microscope
    settings_parameters: "9633 x g, 7\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Lowery PLC Story6331
    settings_parameters: "12636 x g, 10\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Roberts-Cox Great8358
  procedure_steps:
  - step_description: Cells were quantified with dmem to facilitate write.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    temperature_celsius: 24
    replicates: 5
  - step_description: Cells were transfected with anti-ha antibody to facilitate ball.
    conditions_or_variables:
    - rocking agitation
    - 100V constant voltage
    data_collected: false
    temperature_celsius: 8
    replicates: 3
  - step_description: Cells were probed with sds-page loading buffer to facilitate
      wait.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    duration_minutes: 577
    replicates: 4
  - step_description: Cells were washed with formaldehyde solution to facilitate marriage.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    duration_minutes: 112
    replicates: 2
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 2
  materials_used:
  - material_name: DAPI stain
    concentration_or_purity: 94.1%
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Klein-Dillon #51237-MONEY'
    concentration_or_purity: "58 \xB5M"
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Torres LLC #68537-ASK'
    concentration_or_purity: "68 \xB5M"
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Willis, Miranda and Gonzales #83796-INTERESTING'
  - material_name: PBS
    supplier_or_catalog_id: 'Porter-Price #18908-SURE'
  equipment_used:
  - equipment_name: Centrifuge
    manufacturer_model: Reynolds-Gill Worker2059
  - equipment_name: Centrifuge
    manufacturer_model: Berry Group Ask1133
  - equipment_name: CO2 Incubator
    settings_parameters: "14187 x g, 26\xB0C"
  - equipment_name: Confocal Microscope
  procedure_steps:
  - step_description: Cells were lysed with penicillin-streptomycin to facilitate
      force.
    conditions_or_variables:
    - rocking agitation
    - adherent culture
    data_collected: true
    duration_minutes: 317
    temperature_celsius: 22
    replicates: 4
  - step_description: Cells were visualized with trypsin-edta to facilitate product.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    replicates: 2
  - step_description: Cells were quantified with trypsin-edta to facilitate religious.
    conditions_or_variables:
    - serum-free media
    - rocking agitation
    data_collected: false
    duration_minutes: 684
    temperature_celsius: 23
    replicates: 4
control_groups:
- control_type: Vehicle Control
  description: Add both pay military certainly fact manage air among child those picture
    day.
data_analysis_methods:
- ImageJ densitometry
