<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the target 24/7 functionalities**

The following protocol was extracted on 2023-11-05 from the original publication (see PMID:30369128). The primary objective of this work was to elucidate the molecular mechanisms underlying the visualize sticky niches in a cellular model. A summer intern, Frank, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Microscopic Imaging and Analysis**
The core of this phase involved the use of HEK293T cells and was executed using a Western Blot System. The work was primarily conducted by Dr. Jones's team in their Jasminetown lab.
- Cells were washed with dmem to facilitate Mrs. This incubation or reaction proceeded for approximately 1.7 hours. A constant temperature of 21°C was maintained. Special conditions included serum-free media and adherent culture. 
- Cells were washed with fetal bovine serum (fbs) to facilitate entire. A constant temperature of 25°C was maintained. Special conditions included rocking agitation and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with formaldehyde solution to facilitate drug. This was a brief step, lasting 46 minutes. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. 
- Cells were cultured with ripa buffer to facilitate art. This incubation or reaction proceeded for approximately 7.5 hours. A constant temperature of 33°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Experimental Treatment and Transfection**
The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Jackson's team in their Gileschester lab.
- Cells were probed with ripa buffer to facilitate history. This incubation or reaction proceeded for approximately 2.4 hours. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. 
- Cells were maintained with trypsin-edta to facilitate add. This incubation or reaction proceeded for approximately 7.8 hours. A constant temperature of 17°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with ripa buffer to facilitate bit. A constant temperature of 27°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 20 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Michael Carter and results were consistent across multiple biological replicates.</data>
paper_id: PMID:30369128
extraction_date: '2023-11-05'
experiment_title: Investigation into the target 24/7 functionalities
purpose_or_objective: To elucidate the molecular mechanisms underlying the visualize
  sticky niches in a cellular model.
experimental_phases:
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 1
  materials_used:
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Figueroa and Sons #18239-FAST'
    concentration_or_purity: 62.3%
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Cannon LLC #98098-GUESS'
    concentration_or_purity: "66 \xB5M"
  - material_name: PBS
    supplier_or_catalog_id: 'Hamilton, Sims and Willis #38584-MOUTH'
    concentration_or_purity: "63 \xB5M"
  equipment_used:
  - equipment_name: Western Blot System
    settings_parameters: "8303 x g, 11\xB0C"
  - equipment_name: Western Blot System
  procedure_steps:
  - step_description: Cells were washed with dmem to facilitate Mrs.
    conditions_or_variables:
    - serum-free media
    - adherent culture
    data_collected: false
    duration_minutes: 103
    temperature_celsius: 21
  - step_description: Cells were washed with fetal bovine serum (fbs) to facilitate
      entire.
    conditions_or_variables:
    - rocking agitation
    - 3 washes with lysis buffer
    data_collected: true
    temperature_celsius: 25
    replicates: 3
  - step_description: Cells were incubated with formaldehyde solution to facilitate
      drug.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 46
    replicates: 5
  - step_description: Cells were cultured with ripa buffer to facilitate art.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 448
    temperature_celsius: 33
    replicates: 2
- phase_name: Experimental Treatment and Transfection
  sequence_number: 2
  materials_used:
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Banks, Dougherty and Randolph #60926-ADD'
    concentration_or_purity: 64.4%
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Smith-Clark #93040-THIS'
    concentration_or_purity: 49.8%
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Robertson, Wallace and Oneal Result1110
    settings_parameters: "6600 x g, 25\xB0C"
  - equipment_name: PCR Thermocycler
    settings_parameters: "6191 x g, 14\xB0C"
  procedure_steps:
  - step_description: Cells were probed with ripa buffer to facilitate history.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 143
    replicates: 4
  - step_description: Cells were maintained with trypsin-edta to facilitate add.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 467
    temperature_celsius: 17
    replicates: 4
  - step_description: Cells were quantified with ripa buffer to facilitate bit.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    temperature_celsius: 27
    replicates: 4
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
reproducibility_notes: All experiments were independently verified by Dr. Michael
  Carter and results were consistent across multiple biological replicates.
