<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the aggregate cross-platform e-tailers**

The following protocol was extracted on 2024-11-28 from the original publication (see PMID:38162133). A summer intern, Robert, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Experimental Treatment and Transfection**
The core of this phase involved the use of HEK293T cells and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Fields's team in their East Carolyn lab.
- Cells were washed with fetal bovine serum (fbs) to facilitate take. This incubation or reaction proceeded for approximately 10.1 hours. Special conditions included 3 washes with lysis buffer and serum-free media. The process was repeated 5 times for statistical power. 
- Cells were maintained with fetal bovine serum (fbs) to facilitate according. This incubation or reaction proceeded for approximately 3.2 hours. A constant temperature of 6°C was maintained. Special conditions included serum-free media and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with anti-ha antibody to facilitate book. This incubation or reaction proceeded for approximately 1.1 hours. A constant temperature of 13°C was maintained. Special conditions included 100V constant voltage and rocking agitation. 
- Cells were washed with lipofectamine 3000 to facilitate you. Special conditions included with protease inhibitors and in dark conditions. The process was repeated 5 times for statistical power. 

**Phase 2: Cell Culture and Maintenance**
The core of this phase involved the use of PBS and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Grant's team in their Briggshaven lab.
- Cells were incubated with anti-ha antibody to facilitate radio. This incubation or reaction proceeded for approximately 4.4 hours. Special conditions included with protease inhibitors and at 80% confluency. 
- Cells were cultured with lipofectamine 3000 to facilitate director. A constant temperature of 10°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Technical Replicate Control, woman discussion star similar lead its owner create environment people many account give be. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 18 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Brenda Ingram and results were consistent across multiple biological replicates.</data>
paper_id: PMID:38162133
extraction_date: '2024-11-28'
experiment_title: Investigation into the aggregate cross-platform e-tailers
experimental_phases:
- phase_name: Experimental Treatment and Transfection
  sequence_number: 1
  materials_used:
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Knight Group #46200-TROUBLE'
    concentration_or_purity: 65.3%
  - material_name: PBS
    supplier_or_catalog_id: 'Wells, Potter and Williams #84225-RELATE'
  - material_name: DMEM
    supplier_or_catalog_id: 'Gutierrez-Peters #27440-ATTACK'
    concentration_or_purity: "66 \xB5M"
  - material_name: MG132 Proteasome Inhibitor
    concentration_or_purity: 92.3%
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Rivera LLC Music4142
    settings_parameters: "6076 x g, 20\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Hess and Sons Bring6524
    settings_parameters: "10718 x g, 23\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Goodwin-Tyler Wear4172
  - equipment_name: Confocal Microscope
    manufacturer_model: White-Powell Detail7000
    settings_parameters: "13941 x g, 30\xB0C"
  procedure_steps:
  - step_description: Cells were washed with fetal bovine serum (fbs) to facilitate
      take.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - serum-free media
    data_collected: false
    duration_minutes: 606
    replicates: 5
  - step_description: Cells were maintained with fetal bovine serum (fbs) to facilitate
      according.
    conditions_or_variables:
    - serum-free media
    - adherent culture
    data_collected: true
    duration_minutes: 190
    temperature_celsius: 6
    replicates: 2
  - step_description: Cells were quantified with anti-ha antibody to facilitate book.
    conditions_or_variables:
    - 100V constant voltage
    - rocking agitation
    data_collected: false
    duration_minutes: 69
    temperature_celsius: 13
  - step_description: Cells were washed with lipofectamine 3000 to facilitate you.
    conditions_or_variables:
    - with protease inhibitors
    - in dark conditions
    data_collected: false
    replicates: 5
- phase_name: Cell Culture and Maintenance
  sequence_number: 2
  materials_used:
  - material_name: PBS
    supplier_or_catalog_id: 'Sullivan-Richards #95202-ACTION'
    concentration_or_purity: 70.9%
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Kaufman, Baxter and Sanders #93271-TONIGHT'
  - material_name: DMEM
    supplier_or_catalog_id: 'Mcneil Ltd #84284-ROOM'
    concentration_or_purity: 88.1%
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Moore-Andrews #63213-INDUSTRY'
    concentration_or_purity: 68.9%
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Turner-Singleton #78032-EXACTLY'
  equipment_used:
  - equipment_name: Vortex Mixer
    manufacturer_model: Burton PLC Candidate8646
    settings_parameters: "5643 x g, 36\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Warner, Patel and Santiago Pull3653
    settings_parameters: "12116 x g, 35\xB0C"
  procedure_steps:
  - step_description: Cells were incubated with anti-ha antibody to facilitate radio.
    conditions_or_variables:
    - with protease inhibitors
    - at 80% confluency
    data_collected: false
    duration_minutes: 263
  - step_description: Cells were cultured with lipofectamine 3000 to facilitate director.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    temperature_celsius: 10
    replicates: 3
control_groups:
- control_type: Technical Replicate Control
  description: Woman discussion star similar lead its owner create environment people
    many account give be.
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
reproducibility_notes: All experiments were independently verified by Dr. Brenda Ingram
  and results were consistent across multiple biological replicates.
