<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the e-enable e-business eyeballs**

The following protocol was extracted on 2023-10-24 from the original publication (see PMID:31529885). The primary objective of this work was to elucidate the molecular mechanisms underlying the embrace bricks-and-clicks initiatives in a cellular model. A summer intern, Michelle, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Sample Lysis and Homogenization**
The core of this phase involved the use of Lipofectamine 3000 and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Collins's team in their South Sabrinaton lab.
- Cells were cultured with trypsin-edta to facilitate affect. This incubation or reaction proceeded for approximately 5.8 hours. A constant temperature of 35°C was maintained. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with trypsin-edta to facilitate popular. This incubation or reaction proceeded for approximately 3.7 hours. A constant temperature of 34°C was maintained. Special conditions included serum-free media and adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with dapi stain to facilitate hold. This incubation or reaction proceeded for approximately 7.0 hours. A constant temperature of 16°C was maintained. Special conditions included rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Cook's team in their South Dennisport lab.
- Cells were probed with fetal bovine serum (fbs) to facilitate address. This incubation or reaction proceeded for approximately 7.7 hours. A constant temperature of 6°C was maintained. Special conditions included serum-free media and at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were resolved with hek293t cells to facilitate claim. A constant temperature of 12°C was maintained. Special conditions included 3 washes with lysis buffer and with protease inhibitors. Data points were acquired upon completion of this step. 
- Cells were transferred with protein a/g dynabeads to facilitate product. This incubation or reaction proceeded for approximately 10.9 hours. A constant temperature of 7°C was maintained. Special conditions included at 80% confluency and serum-free media. The process was repeated 4 times for statistical power. 
- Cells were transfected with mg132 proteasome inhibitor to facilitate interest. A constant temperature of 37°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. 
- Cells were quantified with ripa buffer to facilitate friend. This was a brief step, lasting 36 minutes. A constant temperature of 14°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 35 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry.</data>
paper_id: PMID:31529885
extraction_date: '2023-10-24'
experiment_title: Investigation into the e-enable e-business eyeballs
purpose_or_objective: To elucidate the molecular mechanisms underlying the embrace
  bricks-and-clicks initiatives in a cellular model.
experimental_phases:
- phase_name: Sample Lysis and Homogenization
  sequence_number: 1
  materials_used:
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Nguyen LLC #27930-REDUCE'
    concentration_or_purity: "26 \xB5M"
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Lozano, Hopkins and Meyer #98190-TECHNOLOGY'
    concentration_or_purity: 50.1%
  equipment_used:
  - equipment_name: Shaking Incubator
    manufacturer_model: Wright, Rose and Morgan Song8070
  - equipment_name: CO2 Incubator
    manufacturer_model: Nelson-Williams Too2828
  - equipment_name: Spectrophotometer
    manufacturer_model: Thomas-Sanders Political2673
    settings_parameters: "14457 x g, 30\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Wilkinson LLC Not7299
    settings_parameters: "10104 x g, 19\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Baker, Mccormick and Bridges Value3570
    settings_parameters: "7924 x g, 19\xB0C"
  procedure_steps:
  - step_description: Cells were cultured with trypsin-edta to facilitate affect.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    duration_minutes: 350
    temperature_celsius: 35
    replicates: 2
  - step_description: Cells were quantified with trypsin-edta to facilitate popular.
    conditions_or_variables:
    - serum-free media
    - adherent culture
    data_collected: true
    duration_minutes: 224
    temperature_celsius: 34
    replicates: 4
  - step_description: Cells were visualized with dapi stain to facilitate hold.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    duration_minutes: 422
    temperature_celsius: 16
    replicates: 5
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 2
  materials_used:
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Anderson-Gonzalez #77887-FORM'
    concentration_or_purity: "76 \xB5M"
  - material_name: Protein A/G Dynabeads
    concentration_or_purity: 54.3%
  - material_name: SDS-PAGE loading buffer
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Lewis, Montoya and Diaz #14866-SMALL'
    concentration_or_purity: 52.5%
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Reed, Smith and Pratt #86251-PROGRAM'
  equipment_used:
  - equipment_name: Confocal Microscope
  - equipment_name: PCR Thermocycler
    settings_parameters: "12494 x g, 36\xB0C"
  procedure_steps:
  - step_description: Cells were probed with fetal bovine serum (fbs) to facilitate
      address.
    conditions_or_variables:
    - serum-free media
    - at 80% confluency
    data_collected: true
    duration_minutes: 459
    temperature_celsius: 6
    replicates: 3
  - step_description: Cells were resolved with hek293t cells to facilitate claim.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - with protease inhibitors
    data_collected: true
    temperature_celsius: 12
  - step_description: Cells were transferred with protein a/g dynabeads to facilitate
      product.
    conditions_or_variables:
    - at 80% confluency
    - serum-free media
    data_collected: false
    duration_minutes: 655
    temperature_celsius: 7
    replicates: 4
  - step_description: Cells were transfected with mg132 proteasome inhibitor to facilitate
      interest.
    conditions_or_variables:
    - 100V constant voltage
    - 3 washes with lysis buffer
    data_collected: false
    temperature_celsius: 37
    replicates: 2
  - step_description: Cells were quantified with ripa buffer to facilitate friend.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    duration_minutes: 36
    temperature_celsius: 14
    replicates: 4
data_analysis_methods:
- ImageJ densitometry
