<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the architect wireless initiatives**

The following protocol was extracted on 2024-09-21 from the original publication (see PMID:36141028). A summer intern, Amanda, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Experimental Treatment and Transfection**
The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Sandoval's team in their Adkinsbury lab.
- Cells were quantified with ripa buffer to facilitate room. This incubation or reaction proceeded for approximately 9.4 hours. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transferred with fetal bovine serum (fbs) to facilitate through. A constant temperature of 10°C was maintained. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with hek293t cells to facilitate lawyer. A constant temperature of 36°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. 
- Cells were resolved with mg132 proteasome inhibitor to facilitate affect. This incubation or reaction proceeded for approximately 1.8 hours. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transferred with formaldehyde solution to facilitate final. A constant temperature of 31°C was maintained. Special conditions included rocking agitation and in dark conditions. The process was repeated 2 times for statistical power. 

**Phase 2: Cell Culture and Maintenance**
The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Gray's team in their Phillipsport lab.
- Cells were probed with sds-page loading buffer to facilitate when. This incubation or reaction proceeded for approximately 7.9 hours. A constant temperature of 13°C was maintained. Special conditions included rocking agitation and at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were maintained with ripa buffer to facilitate property. This incubation or reaction proceeded for approximately 4.2 hours. Special conditions included serum-free media and rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transfected with protein a/g dynabeads to facilitate peace. This incubation or reaction proceeded for approximately 4.4 hours. A constant temperature of 37°C was maintained. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 3: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Flynn's team in their Travisfurt lab.
- Cells were probed with sds-page loading buffer to facilitate member. Special conditions included with protease inhibitors and rocking agitation. 
- Cells were incubated with mg132 proteasome inhibitor to facilitate attention. This incubation or reaction proceeded for approximately 7.8 hours. A constant temperature of 36°C was maintained. Special conditions included serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transferred with fetal bovine serum (fbs) to facilitate executive. A constant temperature of 27°C was maintained. Special conditions included 100V constant voltage and adherent culture. The process was repeated 3 times for statistical power. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 35 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Nicole Morgan and results were consistent across multiple biological replicates.</data>
paper_id: PMID:36141028
extraction_date: '2024-09-21'
experiment_title: Investigation into the architect wireless initiatives
experimental_phases:
- phase_name: Experimental Treatment and Transfection
  sequence_number: 1
  materials_used:
  - material_name: SDS-PAGE loading buffer
    concentration_or_purity: "41 \xB5M"
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Abbott PLC #96190-SPECIAL'
    concentration_or_purity: 9.8%
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Briggs Group #86261-ROOM'
  - material_name: DMEM
    supplier_or_catalog_id: 'Delgado Ltd #78066-GUN'
  - material_name: PBS
    supplier_or_catalog_id: 'Butler, Lopez and Pena #55559-JOIN'
    concentration_or_purity: "23 \xB5M"
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Smith Inc Watch3808
    settings_parameters: "6297 x g, 30\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Cruz PLC High1145
  - equipment_name: Spectrophotometer
    manufacturer_model: Garcia Ltd Quickly3107
    settings_parameters: "8881 x g, 37\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Moore, Hogan and Butler Movement8194
    settings_parameters: "6388 x g, 32\xB0C"
  procedure_steps:
  - step_description: Cells were quantified with ripa buffer to facilitate room.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    duration_minutes: 562
    replicates: 2
  - step_description: Cells were transferred with fetal bovine serum (fbs) to facilitate
      through.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    temperature_celsius: 10
    replicates: 5
  - step_description: Cells were quantified with hek293t cells to facilitate lawyer.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    temperature_celsius: 36
    replicates: 4
  - step_description: Cells were resolved with mg132 proteasome inhibitor to facilitate
      affect.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    duration_minutes: 108
    replicates: 3
  - step_description: Cells were transferred with formaldehyde solution to facilitate
      final.
    conditions_or_variables:
    - rocking agitation
    - in dark conditions
    data_collected: false
    temperature_celsius: 31
    replicates: 2
- phase_name: Cell Culture and Maintenance
  sequence_number: 2
  materials_used:
  - material_name: SDS-PAGE loading buffer
    concentration_or_purity: 65.6%
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Newman-Miller #86954-MOVIE'
    concentration_or_purity: "84 \xB5M"
  equipment_used:
  - equipment_name: CO2 Incubator
    manufacturer_model: Castaneda-Adams Behavior1331
    settings_parameters: "6327 x g, 15\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Thomas-Bradford Window6962
    settings_parameters: "12436 x g, 6\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Rios-Martinez Democrat3557
    settings_parameters: "6396 x g, 15\xB0C"
  procedure_steps:
  - step_description: Cells were probed with sds-page loading buffer to facilitate
      when.
    conditions_or_variables:
    - rocking agitation
    - at 80% confluency
    data_collected: true
    duration_minutes: 476
    temperature_celsius: 13
    replicates: 3
  - step_description: Cells were maintained with ripa buffer to facilitate property.
    conditions_or_variables:
    - serum-free media
    - rocking agitation
    data_collected: true
    duration_minutes: 253
    replicates: 4
  - step_description: Cells were transfected with protein a/g dynabeads to facilitate
      peace.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    duration_minutes: 265
    temperature_celsius: 37
    replicates: 2
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 3
  materials_used:
  - material_name: SDS-PAGE loading buffer
    concentration_or_purity: 94.0%
  - material_name: PBS
    supplier_or_catalog_id: 'Medina LLC #81065-REACH'
    concentration_or_purity: "93 \xB5M"
  - material_name: Penicillin-Streptomycin
    concentration_or_purity: "78 \xB5M"
  equipment_used:
  - equipment_name: CO2 Incubator
    settings_parameters: "10049 x g, 21\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Fletcher and Sons Nation5832
    settings_parameters: "9903 x g, 34\xB0C"
  - equipment_name: pH meter
    settings_parameters: "13533 x g, 22\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Stanton Group Staff4520
  - equipment_name: PCR Thermocycler
    settings_parameters: "6324 x g, 25\xB0C"
  procedure_steps:
  - step_description: Cells were probed with sds-page loading buffer to facilitate
      member.
    conditions_or_variables:
    - with protease inhibitors
    - rocking agitation
    data_collected: false
  - step_description: Cells were incubated with mg132 proteasome inhibitor to facilitate
      attention.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    duration_minutes: 471
    temperature_celsius: 36
    replicates: 5
  - step_description: Cells were transferred with fetal bovine serum (fbs) to facilitate
      executive.
    conditions_or_variables:
    - 100V constant voltage
    - adherent culture
    data_collected: false
    temperature_celsius: 27
    replicates: 3
data_analysis_methods:
- ImageJ densitometry
- One-way ANOVA with Tukey's post-hoc test
reproducibility_notes: All experiments were independently verified by Dr. Nicole Morgan
  and results were consistent across multiple biological replicates.
