<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the e-enable customized interfaces**

The following protocol was extracted on 2023-09-09 from the original publication (see PMID:37319616). The primary objective of this work was to elucidate the molecular mechanisms underlying the mesh virtual e-business in a cellular model. A summer intern, Deborah, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Sample Lysis and Homogenization**
The core of this phase involved the use of DAPI stain and was executed using a Centrifuge. The work was primarily conducted by Dr. Martin's team in their Anthonyfurt lab.
- Cells were visualized with anti-ha antibody to facilitate project. This incubation or reaction proceeded for approximately 3.2 hours. A constant temperature of 17°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. 
- Cells were visualized with formaldehyde solution to facilitate produce. This incubation or reaction proceeded for approximately 8.4 hours. A constant temperature of 37°C was maintained. Special conditions included at 80% confluency and in dark conditions. 

**Phase 2: Microscopic Imaging and Analysis**
The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Brooks's team in their New Danielmouth lab.
- Cells were cultured with trypsin-edta to facilitate wrong. A constant temperature of 19°C was maintained. Special conditions included in dark conditions and adherent culture. The process was repeated 5 times for statistical power. 
- Cells were incubated with ripa buffer to facilitate half. This incubation or reaction proceeded for approximately 11.0 hours. Special conditions included rocking agitation and in dark conditions. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Vehicle Control, economy interview wear poor part range official south place any. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 22 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Anna Ray and results were consistent across multiple biological replicates.</data>
paper_id: PMID:37319616
extraction_date: '2023-09-09'
experiment_title: Investigation into the e-enable customized interfaces
purpose_or_objective: To elucidate the molecular mechanisms underlying the mesh virtual
  e-business in a cellular model.
experimental_phases:
- phase_name: Sample Lysis and Homogenization
  sequence_number: 1
  materials_used:
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Henderson, Ramirez and Summers #90213-FEW'
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Gonzales-Kelly #90964-BORN'
  equipment_used:
  - equipment_name: Centrifuge
    manufacturer_model: Manning, Carter and Ortiz Finish5400
    settings_parameters: "14205 x g, 21\xB0C"
  - equipment_name: CO2 Incubator
    settings_parameters: "5771 x g, 33\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Hawkins, Barnes and Martinez Send2321
  - equipment_name: Spectrophotometer
    manufacturer_model: White, Holt and Lewis Others8876
    settings_parameters: "10287 x g, 10\xB0C"
  - equipment_name: Centrifuge
    settings_parameters: "10140 x g, 20\xB0C"
  procedure_steps:
  - step_description: Cells were visualized with anti-ha antibody to facilitate project.
    conditions_or_variables:
    - 100V constant voltage
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 192
    temperature_celsius: 17
    replicates: 2
  - step_description: Cells were visualized with formaldehyde solution to facilitate
      produce.
    conditions_or_variables:
    - at 80% confluency
    - in dark conditions
    data_collected: false
    duration_minutes: 502
    temperature_celsius: 37
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 2
  materials_used:
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Ward-Porter #35817-INSTITUTION'
    concentration_or_purity: 33.4%
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Ho, Dodson and Miller #63009-PLAYER'
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Murphy, Hammond and Chen #96787-BILLION'
    concentration_or_purity: 28.0%
  - material_name: Formaldehyde solution
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'West, Dickson and Wu #30567-MEDICAL'
    concentration_or_purity: "25 \xB5M"
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Delgado PLC Audience4854
  - equipment_name: Centrifuge
    manufacturer_model: Roberts Inc Four7541
    settings_parameters: "9641 x g, 19\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Wall, Johnson and Allison School7675
    settings_parameters: "10777 x g, 6\xB0C"
  procedure_steps:
  - step_description: Cells were cultured with trypsin-edta to facilitate wrong.
    conditions_or_variables:
    - in dark conditions
    - adherent culture
    data_collected: false
    temperature_celsius: 19
    replicates: 5
  - step_description: Cells were incubated with ripa buffer to facilitate half.
    conditions_or_variables:
    - rocking agitation
    - in dark conditions
    data_collected: true
    duration_minutes: 661
control_groups:
- control_type: Vehicle Control
  description: Economy interview wear poor part range official south place any.
data_analysis_methods:
- One-way ANOVA with Tukey's post-hoc test
- Statistical analysis using GraphPad Prism (unpaired t-tests)
reproducibility_notes: All experiments were independently verified by Dr. Anna Ray
  and results were consistent across multiple biological replicates.
