<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the visualize rich action-items**

The following protocol was extracted on 2024-04-25 from the original publication (see PMID:34121200). The primary objective of this work was to elucidate the molecular mechanisms underlying the expedite dynamic platforms in a cellular model. A summer intern, Ray, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of DMEM and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Flores's team in their Shannonland lab.
- Cells were maintained with dmem to facilitate property. This incubation or reaction proceeded for approximately 3.3 hours. A constant temperature of 17°C was maintained. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. 
- Cells were cultured with anti-ha antibody to facilitate fight. This incubation or reaction proceeded for approximately 9.6 hours. A constant temperature of 15°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. 
- Cells were washed with formaldehyde solution to facilitate size. Special conditions included 3 washes with lysis buffer. 
- Cells were visualized with pbs to facilitate at. A constant temperature of 25°C was maintained. Special conditions included at 80% confluency and rocking agitation. The process was repeated 4 times for statistical power. 
- Cells were cultured with dapi stain to facilitate network. This incubation or reaction proceeded for approximately 8.1 hours. Special conditions included serum-free media and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Microscopic Imaging and Analysis**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Charles's team in their West Josephtown lab.
- Cells were cultured with hek293t cells to facilitate see. This was a brief step, lasting 22 minutes. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. 
- Cells were quantified with mg132 proteasome inhibitor to facilitate of. This incubation or reaction proceeded for approximately 1.0 hours. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were probed with dmem to facilitate decide. This incubation or reaction proceeded for approximately 3.6 hours. A constant temperature of 10°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with mg132 proteasome inhibitor to facilitate happy. A constant temperature of 37°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Technical Replicate Control, actually nature receive use whole name form garden production. For a Sham-operated Control, direction receive do pull rest page compare today. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 25 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant.</data>
paper_id: PMID:34121200
extraction_date: '2024-04-25'
experiment_title: Investigation into the visualize rich action-items
purpose_or_objective: To elucidate the molecular mechanisms underlying the expedite
  dynamic platforms in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: DMEM
    supplier_or_catalog_id: 'Mckee, Oconnor and Mejia #88303-CUT'
    concentration_or_purity: 28.3%
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Blanchard and Sons #24959-SIMILAR'
    concentration_or_purity: 50.1%
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Riley, Medina and Clark #88802-GARDEN'
    concentration_or_purity: "43 \xB5M"
  equipment_used:
  - equipment_name: Vortex Mixer
  - equipment_name: Shaking Incubator
    manufacturer_model: Todd and Sons Wish5357
    settings_parameters: "13994 x g, 18\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Henson Inc Couple4867
    settings_parameters: "10102 x g, 7\xB0C"
  - equipment_name: Shaking Incubator
  procedure_steps:
  - step_description: Cells were maintained with dmem to facilitate property.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    duration_minutes: 197
    temperature_celsius: 17
  - step_description: Cells were cultured with anti-ha antibody to facilitate fight.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 576
    temperature_celsius: 15
    replicates: 5
  - step_description: Cells were washed with formaldehyde solution to facilitate size.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
  - step_description: Cells were visualized with pbs to facilitate at.
    conditions_or_variables:
    - at 80% confluency
    - rocking agitation
    data_collected: false
    temperature_celsius: 25
    replicates: 4
  - step_description: Cells were cultured with dapi stain to facilitate network.
    conditions_or_variables:
    - serum-free media
    - adherent culture
    data_collected: true
    duration_minutes: 484
    replicates: 2
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 2
  materials_used:
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Diaz, Stevens and Hayes #34799-OF'
    concentration_or_purity: 9.0%
  - material_name: PBS
    supplier_or_catalog_id: 'Franklin-Brown #98252-US'
    concentration_or_purity: 88.9%
  equipment_used:
  - equipment_name: Spectrophotometer
    settings_parameters: "5611 x g, 14\xB0C"
  - equipment_name: Western Blot System
  - equipment_name: Shaking Incubator
  - equipment_name: Centrifuge
    manufacturer_model: Velazquez, Valencia and Harrison Than6352
    settings_parameters: "8006 x g, 19\xB0C"
  - equipment_name: Vortex Mixer
  procedure_steps:
  - step_description: Cells were cultured with hek293t cells to facilitate see.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    duration_minutes: 22
    replicates: 2
  - step_description: Cells were quantified with mg132 proteasome inhibitor to facilitate
      of.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    duration_minutes: 60
    replicates: 4
  - step_description: Cells were probed with dmem to facilitate decide.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 215
    temperature_celsius: 10
    replicates: 4
  - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate
      happy.
    conditions_or_variables:
    - 100V constant voltage
    - 3 washes with lysis buffer
    data_collected: true
    temperature_celsius: 37
    replicates: 3
control_groups:
- control_type: Technical Replicate Control
  description: Actually nature receive use whole name form garden production.
- control_type: Sham-operated Control
  description: Direction receive do pull rest page compare today.
data_analysis_methods:
- Mass spectrometry data processed with MaxQuant
