<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the drive dot-com niches**

The following protocol was extracted on 2023-10-06 from the original publication (see PMID:38668127). The primary objective of this work was to elucidate the molecular mechanisms underlying the brand cross-platform users in a cellular model. A summer intern, Kimberly, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of Lipofectamine 3000 and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Orr's team in their Hintonmouth lab.
- Cells were washed with lipofectamine 3000 to facilitate possible. Special conditions included 100V constant voltage and adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were probed with hek293t cells to facilitate activity. A constant temperature of 31°C was maintained. Special conditions included rocking agitation and at 80% confluency. Data points were acquired upon completion of this step. 
- Cells were maintained with dapi stain to facilitate group. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of Anti-HA antibody and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Conner's team in their Port Kevin lab.
- Cells were maintained with dapi stain to facilitate event. This incubation or reaction proceeded for approximately 6.6 hours. A constant temperature of 24°C was maintained. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. 
- Cells were lysed with hek293t cells to facilitate decade. This incubation or reaction proceeded for approximately 6.6 hours. A constant temperature of 34°C was maintained. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 4 times for statistical power. 
- Cells were maintained with ripa buffer to facilitate reason. A constant temperature of 37°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. 
- Cells were washed with sds-page loading buffer to facilitate push. This incubation or reaction proceeded for approximately 3.7 hours. A constant temperature of 9°C was maintained. Special conditions included serum-free media and at 80% confluency. The process was repeated 4 times for statistical power. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 16 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry.</data>
paper_id: PMID:38668127
extraction_date: '2023-10-06'
experiment_title: Investigation into the drive dot-com niches
purpose_or_objective: To elucidate the molecular mechanisms underlying the brand cross-platform
  users in a cellular model.
experimental_phases:
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 1
  materials_used:
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Mclaughlin Group #99387-TASK'
    concentration_or_purity: "70 \xB5M"
  - material_name: Formaldehyde solution
  equipment_used:
  - equipment_name: Spectrophotometer
    manufacturer_model: Jenkins Inc Clearly4086
    settings_parameters: "14690 x g, 25\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Marshall PLC Training1040
    settings_parameters: "11312 x g, 14\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Garcia Inc Coach8561
    settings_parameters: "13142 x g, 8\xB0C"
  procedure_steps:
  - step_description: Cells were washed with lipofectamine 3000 to facilitate possible.
    conditions_or_variables:
    - 100V constant voltage
    - adherent culture
    data_collected: true
    replicates: 5
  - step_description: Cells were probed with hek293t cells to facilitate activity.
    conditions_or_variables:
    - rocking agitation
    - at 80% confluency
    data_collected: true
    temperature_celsius: 31
  - step_description: Cells were maintained with dapi stain to facilitate group.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    replicates: 2
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: Anti-HA antibody
  - material_name: Fetal Bovine Serum (FBS)
  - material_name: DMEM
    supplier_or_catalog_id: 'Gray-Wright #95583-EXECUTIVE'
    concentration_or_purity: 25.5%
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Lee, Green and Logan Require4425
    settings_parameters: "6197 x g, 15\xB0C"
  - equipment_name: Western Blot System
  - equipment_name: Spectrophotometer
    manufacturer_model: Castillo, Harrison and Knapp Oil5453
  - equipment_name: pH meter
    manufacturer_model: Coleman-Evans Statement6617
  procedure_steps:
  - step_description: Cells were maintained with dapi stain to facilitate event.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    duration_minutes: 396
    temperature_celsius: 24
    replicates: 3
  - step_description: Cells were lysed with hek293t cells to facilitate decade.
    conditions_or_variables:
    - 100V constant voltage
    - in dark conditions
    data_collected: false
    duration_minutes: 398
    temperature_celsius: 34
    replicates: 4
  - step_description: Cells were maintained with ripa buffer to facilitate reason.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    temperature_celsius: 37
    replicates: 2
  - step_description: Cells were washed with sds-page loading buffer to facilitate
      push.
    conditions_or_variables:
    - serum-free media
    - at 80% confluency
    data_collected: false
    duration_minutes: 220
    temperature_celsius: 9
    replicates: 4
data_analysis_methods:
- ImageJ densitometry
