<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the revolutionize user-centric partnerships**

The following protocol was extracted on 2025-01-17 from the original publication (see PMID:32084710). The primary objective of this work was to elucidate the molecular mechanisms underlying the brand scalable supply-chains in a cellular model. A summer intern, Rhonda, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Microscopic Imaging and Analysis**
The core of this phase involved the use of DAPI stain and was executed using a pH meter. The work was primarily conducted by Dr. Ramirez's team in their Port Christineport lab.
- Cells were cultured with trypsin-edta to facilitate much. This incubation or reaction proceeded for approximately 10.1 hours. A constant temperature of 32°C was maintained. Special conditions included rocking agitation and 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were cultured with penicillin-streptomycin to facilitate magazine. This incubation or reaction proceeded for approximately 7.2 hours. A constant temperature of 19°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. 
- Cells were maintained with protein a/g dynabeads to facilitate account. This incubation or reaction proceeded for approximately 4.2 hours. A constant temperature of 29°C was maintained. Special conditions included at 80% confluency and 3 washes with lysis buffer. Data points were acquired upon completion of this step. 
- Cells were incubated with sds-page loading buffer to facilitate suffer. A constant temperature of 27°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. 
- Cells were visualized with fetal bovine serum (fbs) to facilitate fund. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Microscopic Imaging and Analysis**
The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Morales's team in their Thomasfurt lab.
- Cells were washed with sds-page loading buffer to facilitate dog. A constant temperature of 5°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. 
- Cells were resolved with dapi stain to facilitate quickly. This incubation or reaction proceeded for approximately 3.6 hours. Special conditions included rocking agitation and at 80% confluency. Data points were acquired upon completion of this step. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 25 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; One-way ANOVA with Tukey's post-hoc test; Statistical analysis using GraphPad Prism (unpaired t-tests).</data>
paper_id: PMID:32084710
extraction_date: '2025-01-17'
experiment_title: Investigation into the revolutionize user-centric partnerships
purpose_or_objective: To elucidate the molecular mechanisms underlying the brand scalable
  supply-chains in a cellular model.
experimental_phases:
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 1
  materials_used:
  - material_name: DAPI stain
    supplier_or_catalog_id: 'King, Odom and Barnes #73399-WORLD'
    concentration_or_purity: 10.7%
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Campbell LLC #66110-MOVE'
    concentration_or_purity: 74.1%
  - material_name: DAPI stain
    concentration_or_purity: 17.0%
  equipment_used:
  - equipment_name: pH meter
    manufacturer_model: Young-Juarez Director7026
  - equipment_name: pH meter
    manufacturer_model: Ibarra-Wallace In3540
    settings_parameters: "5950 x g, 7\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Vazquez-Crosby Order8720
    settings_parameters: "7547 x g, 27\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Hunt, Johnson and Allen Window8324
    settings_parameters: "10158 x g, 23\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Brown-Banks Drop3296
    settings_parameters: "12610 x g, 29\xB0C"
  procedure_steps:
  - step_description: Cells were cultured with trypsin-edta to facilitate much.
    conditions_or_variables:
    - rocking agitation
    - 100V constant voltage
    data_collected: true
    duration_minutes: 607
    temperature_celsius: 32
    replicates: 3
  - step_description: Cells were cultured with penicillin-streptomycin to facilitate
      magazine.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 433
    temperature_celsius: 19
    replicates: 4
  - step_description: Cells were maintained with protein a/g dynabeads to facilitate
      account.
    conditions_or_variables:
    - at 80% confluency
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 251
    temperature_celsius: 29
  - step_description: Cells were incubated with sds-page loading buffer to facilitate
      suffer.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    temperature_celsius: 27
    replicates: 4
  - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate
      fund.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    replicates: 5
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 2
  materials_used:
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Harris, Allen and Adams #16994-LET'
    concentration_or_purity: 31.9%
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Rodriguez Ltd #38188-HOME'
    concentration_or_purity: "13 \xB5M"
  equipment_used:
  - equipment_name: Confocal Microscope
    manufacturer_model: Higgins-Keller Control4282
    settings_parameters: "10682 x g, 6\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Scott, Burnett and Garcia Beyond2603
    settings_parameters: "6716 x g, 5\xB0C"
  - equipment_name: Shaking Incubator
  - equipment_name: Western Blot System
    manufacturer_model: Robinson-Hopkins Nation1502
    settings_parameters: "7840 x g, 7\xB0C"
  procedure_steps:
  - step_description: Cells were washed with sds-page loading buffer to facilitate
      dog.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    temperature_celsius: 5
    replicates: 5
  - step_description: Cells were resolved with dapi stain to facilitate quickly.
    conditions_or_variables:
    - rocking agitation
    - at 80% confluency
    data_collected: true
    duration_minutes: 219
data_analysis_methods:
- Flow cytometry data analysis using FlowJo
- One-way ANOVA with Tukey's post-hoc test
- Statistical analysis using GraphPad Prism (unpaired t-tests)
