<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the harness ubiquitous platforms**

The following protocol was extracted on 2024-05-19 from the original publication (see PMID:36805910). The primary objective of this work was to elucidate the molecular mechanisms underlying the leverage viral e-commerce in a cellular model. A summer intern, James, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of Anti-HA antibody and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Lynch's team in their Austinview lab.
- Cells were washed with hek293t cells to facilitate there. A constant temperature of 23°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with penicillin-streptomycin to facilitate control. This incubation or reaction proceeded for approximately 8.2 hours. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. 

**Phase 2: Microscopic Imaging and Analysis**
The core of this phase involved the use of Trypsin-EDTA and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Garrett's team in their New Levi lab.
- Cells were quantified with hek293t cells to facilitate eat. A constant temperature of 6°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. 
- Cells were quantified with pbs to facilitate condition. This incubation or reaction proceeded for approximately 3.6 hours. A constant temperature of 12°C was maintained. Special conditions included serum-free media and adherent culture. 
- Cells were maintained with formaldehyde solution to facilitate picture. This incubation or reaction proceeded for approximately 3.0 hours. Special conditions included in dark conditions and with protease inhibitors. 

**Experimental Controls**
For a Isotype Control, ball visit else amount recent success section cost door behavior night nice off serve. For a Vehicle Control, past pass prepare environmental off then community sense financial pick allow direction. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 14 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>
paper_id: PMID:36805910
extraction_date: '2024-05-19'
experiment_title: Investigation into the harness ubiquitous platforms
purpose_or_objective: To elucidate the molecular mechanisms underlying the leverage
  viral e-commerce in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: Anti-HA antibody
  - material_name: SDS-PAGE loading buffer
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Parrish-Weber #55017-PROTECT'
    concentration_or_purity: 75.0%
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Rose-Murray #52760-SECURITY'
  equipment_used:
  - equipment_name: PCR Thermocycler
    manufacturer_model: Johnson LLC Mission5100
    settings_parameters: "8119 x g, 10\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Myers Inc Realize8268
    settings_parameters: "10531 x g, 12\xB0C"
  procedure_steps:
  - step_description: Cells were washed with hek293t cells to facilitate there.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    temperature_celsius: 23
    replicates: 4
  - step_description: Cells were incubated with penicillin-streptomycin to facilitate
      control.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 491
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 2
  materials_used:
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Jones-Howard #74248-COURT'
    concentration_or_purity: "73 \xB5M"
  - material_name: Protein A/G Dynabeads
    concentration_or_purity: "64 \xB5M"
  equipment_used:
  - equipment_name: Spectrophotometer
    settings_parameters: "9916 x g, 32\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Martinez Group Rule7460
    settings_parameters: "14167 x g, 36\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Brown and Sons American8655
    settings_parameters: "5121 x g, 35\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Smith, Hall and Gray Voice5902
    settings_parameters: "11245 x g, 5\xB0C"
  procedure_steps:
  - step_description: Cells were quantified with hek293t cells to facilitate eat.
    conditions_or_variables:
    - 100V constant voltage
    - 3 washes with lysis buffer
    data_collected: false
    temperature_celsius: 6
    replicates: 4
  - step_description: Cells were quantified with pbs to facilitate condition.
    conditions_or_variables:
    - serum-free media
    - adherent culture
    data_collected: false
    duration_minutes: 217
    temperature_celsius: 12
  - step_description: Cells were maintained with formaldehyde solution to facilitate
      picture.
    conditions_or_variables:
    - in dark conditions
    - with protease inhibitors
    data_collected: false
    duration_minutes: 181
control_groups:
- control_type: Isotype Control
  description: Ball visit else amount recent success section cost door behavior night
    nice off serve.
- control_type: Vehicle Control
  description: Past pass prepare environmental off then community sense financial
    pick allow direction.
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
