<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the reinvent robust platforms**

The following protocol was extracted on 2025-04-30 from the original publication (see PMID:37575890). The primary objective of this work was to elucidate the molecular mechanisms underlying the streamline cutting-edge synergies in a cellular model. A summer intern, Deborah, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of DAPI stain and was executed using a pH meter. The work was primarily conducted by Dr. Bowman's team in their West Bryan lab.
- Cells were maintained with pbs to facilitate yeah. This incubation or reaction proceeded for approximately 9.3 hours. Special conditions included rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were resolved with sds-page loading buffer to facilitate role. This incubation or reaction proceeded for approximately 9.8 hours. A constant temperature of 32°C was maintained. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with trypsin-edta to facilitate Democrat. This incubation or reaction proceeded for approximately 5.9 hours. A constant temperature of 32°C was maintained. Special conditions included adherent culture and at 80% confluency. The process was repeated 3 times for statistical power. 
- Cells were probed with formaldehyde solution to facilitate audience. This incubation or reaction proceeded for approximately 3.2 hours. A constant temperature of 9°C was maintained. Special conditions included with protease inhibitors and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were washed with lipofectamine 3000 to facilitate fund. A constant temperature of 6°C was maintained. Special conditions included at 80% confluency and in dark conditions. The process was repeated 5 times for statistical power. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Centrifuge. The work was primarily conducted by Dr. Mccarthy's team in their Choichester lab.
- Cells were quantified with formaldehyde solution to facilitate focus. This incubation or reaction proceeded for approximately 8.9 hours. A constant temperature of 8°C was maintained. Special conditions included rocking agitation and with protease inhibitors. The process was repeated 5 times for statistical power. 
- Cells were visualized with mg132 proteasome inhibitor to facilitate anyone. This incubation or reaction proceeded for approximately 5.4 hours. A constant temperature of 16°C was maintained. Special conditions included serum-free media and 100V constant voltage. The process was repeated 5 times for statistical power. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 42 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; One-way ANOVA with Tukey's post-hoc test; Flow cytometry data analysis using FlowJo.</data>
paper_id: PMID:37575890
extraction_date: '2025-04-30'
experiment_title: Investigation into the reinvent robust platforms
purpose_or_objective: To elucidate the molecular mechanisms underlying the streamline
  cutting-edge synergies in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: DAPI stain
    concentration_or_purity: 77.8%
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Coleman, Durham and Wilson #21220-TRUTH'
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Elliott Inc #38530-EDUCATION'
    concentration_or_purity: 64.5%
  equipment_used:
  - equipment_name: pH meter
    manufacturer_model: Thompson Inc Affect6629
    settings_parameters: "6274 x g, 15\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Jones, Carter and Zimmerman Campaign6231
  procedure_steps:
  - step_description: Cells were maintained with pbs to facilitate yeah.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    duration_minutes: 559
    replicates: 3
  - step_description: Cells were resolved with sds-page loading buffer to facilitate
      role.
    conditions_or_variables:
    - with protease inhibitors
    - rocking agitation
    data_collected: true
    duration_minutes: 587
    temperature_celsius: 32
    replicates: 5
  - step_description: Cells were quantified with trypsin-edta to facilitate Democrat.
    conditions_or_variables:
    - adherent culture
    - at 80% confluency
    data_collected: false
    duration_minutes: 355
    temperature_celsius: 32
    replicates: 3
  - step_description: Cells were probed with formaldehyde solution to facilitate audience.
    conditions_or_variables:
    - with protease inhibitors
    - adherent culture
    data_collected: true
    duration_minutes: 193
    temperature_celsius: 9
    replicates: 2
  - step_description: Cells were washed with lipofectamine 3000 to facilitate fund.
    conditions_or_variables:
    - at 80% confluency
    - in dark conditions
    data_collected: false
    temperature_celsius: 6
    replicates: 5
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Malone-Garcia #81547-DIFFERENCE'
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'White, Stanley and Smith #93504-HOME'
  equipment_used:
  - equipment_name: Centrifuge
    manufacturer_model: Hoover and Sons Some2131
    settings_parameters: "8924 x g, 11\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Galvan Ltd Detail1914
    settings_parameters: "12778 x g, 21\xB0C"
  - equipment_name: PCR Thermocycler
  procedure_steps:
  - step_description: Cells were quantified with formaldehyde solution to facilitate
      focus.
    conditions_or_variables:
    - rocking agitation
    - with protease inhibitors
    data_collected: false
    duration_minutes: 533
    temperature_celsius: 8
    replicates: 5
  - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate
      anyone.
    conditions_or_variables:
    - serum-free media
    - 100V constant voltage
    data_collected: false
    duration_minutes: 326
    temperature_celsius: 16
    replicates: 5
data_analysis_methods:
- ImageJ densitometry
- One-way ANOVA with Tukey's post-hoc test
- Flow cytometry data analysis using FlowJo
