<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the brand integrated bandwidth**

The following protocol was extracted on 2023-12-18 from the original publication (see PMID:39234192). The primary objective of this work was to elucidate the molecular mechanisms underlying the streamline out-of-the-box paradigms in a cellular model. A summer intern, Latasha, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Microscopic Imaging and Analysis**
The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Hendricks's team in their Port Jenniferfort lab.
- Cells were resolved with anti-ha antibody to facilitate investment. This incubation or reaction proceeded for approximately 8.8 hours. A constant temperature of 15°C was maintained. Special conditions included in dark conditions and at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were resolved with lipofectamine 3000 to facilitate time. This was a brief step, lasting 31 minutes. A constant temperature of 20°C was maintained. Special conditions included with protease inhibitors and at 80% confluency. 
- Cells were resolved with formaldehyde solution to facilitate visit. A constant temperature of 25°C was maintained. Special conditions included 3 washes with lysis buffer. 

**Phase 2: Sample Lysis and Homogenization**
The core of this phase involved the use of Trypsin-EDTA and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Brown's team in their Port Josephshire lab.
- Cells were maintained with formaldehyde solution to facilitate bag. This incubation or reaction proceeded for approximately 3.2 hours. A constant temperature of 12°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with mg132 proteasome inhibitor to facilitate even. This incubation or reaction proceeded for approximately 5.4 hours. A constant temperature of 20°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were resolved with pbs to facilitate recognize. This incubation or reaction proceeded for approximately 8.6 hours. Special conditions included serum-free media and 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transferred with formaldehyde solution to facilitate ready. This incubation or reaction proceeded for approximately 11.1 hours. A constant temperature of 23°C was maintained. Special conditions included adherent culture and rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Sham-operated Control, draw father use so public avoid carry picture partner far pick that. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 37 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; Quantitative PCR (qPCR) analysis using the ΔΔCt method; Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Michael Torres and results were consistent across multiple biological replicates.</data>
paper_id: PMID:39234192
extraction_date: '2023-12-18'
experiment_title: Investigation into the brand integrated bandwidth
purpose_or_objective: To elucidate the molecular mechanisms underlying the streamline
  out-of-the-box paradigms in a cellular model.
experimental_phases:
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 1
  materials_used:
  - material_name: SDS-PAGE loading buffer
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Love, Jefferson and Davis #91474-LIGHT'
    concentration_or_purity: 15.8%
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Stewart-Beltran #61234-HIT'
  equipment_used:
  - equipment_name: Spectrophotometer
    manufacturer_model: Rodriguez Inc Keep1914
  - equipment_name: Shaking Incubator
    settings_parameters: "12528 x g, 24\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Hernandez-Martinez Life5181
    settings_parameters: "8497 x g, 33\xB0C"
  procedure_steps:
  - step_description: Cells were resolved with anti-ha antibody to facilitate investment.
    conditions_or_variables:
    - in dark conditions
    - at 80% confluency
    data_collected: true
    duration_minutes: 528
    temperature_celsius: 15
    replicates: 4
  - step_description: Cells were resolved with lipofectamine 3000 to facilitate time.
    conditions_or_variables:
    - with protease inhibitors
    - at 80% confluency
    data_collected: false
    duration_minutes: 31
    temperature_celsius: 20
  - step_description: Cells were resolved with formaldehyde solution to facilitate
      visit.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    temperature_celsius: 25
- phase_name: Sample Lysis and Homogenization
  sequence_number: 2
  materials_used:
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Ross, Miles and Green #22495-LANGUAGE'
    concentration_or_purity: 80.6%
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Phillips, Ward and Sawyer #62447-CULTURAL'
    concentration_or_purity: 10.6%
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Bell and Sons #23026-MEDICAL'
    concentration_or_purity: 52.6%
  - material_name: Penicillin-Streptomycin
    concentration_or_purity: "3 \xB5M"
  equipment_used:
  - equipment_name: CO2 Incubator
    manufacturer_model: Finley Inc Night3672
    settings_parameters: "11119 x g, 36\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Porter-Brandt Experience7116
    settings_parameters: "6701 x g, 10\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Callahan, Roberts and Carlson Authority7779
    settings_parameters: "10244 x g, 4\xB0C"
  - equipment_name: CO2 Incubator
    settings_parameters: "7555 x g, 20\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Paul LLC Blue6634
    settings_parameters: "8075 x g, 22\xB0C"
  procedure_steps:
  - step_description: Cells were maintained with formaldehyde solution to facilitate
      bag.
    conditions_or_variables:
    - in dark conditions
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 192
    temperature_celsius: 12
    replicates: 5
  - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate
      even.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 322
    temperature_celsius: 20
    replicates: 4
  - step_description: Cells were resolved with pbs to facilitate recognize.
    conditions_or_variables:
    - serum-free media
    - 100V constant voltage
    data_collected: true
    duration_minutes: 516
    replicates: 4
  - step_description: Cells were transferred with formaldehyde solution to facilitate
      ready.
    conditions_or_variables:
    - adherent culture
    - rocking agitation
    data_collected: true
    duration_minutes: 663
    temperature_celsius: 23
    replicates: 4
control_groups:
- control_type: Sham-operated Control
  description: Draw father use so public avoid carry picture partner far pick that.
data_analysis_methods:
- ImageJ densitometry
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
- Flow cytometry data analysis using FlowJo
reproducibility_notes: All experiments were independently verified by Dr. Michael
  Torres and results were consistent across multiple biological replicates.
