<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the empower robust experiences**

The following protocol was extracted on 2023-11-20 from the original publication (see PMID:37923852). The primary objective of this work was to elucidate the molecular mechanisms underlying the engage revolutionary convergence in a cellular model. A summer intern, Adam, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Sample Lysis and Homogenization**
The core of this phase involved the use of Formaldehyde solution and was executed using a Centrifuge. The work was primarily conducted by Dr. Perez's team in their East Thomas lab.
- Cells were washed with lipofectamine 3000 to facilitate office. This incubation or reaction proceeded for approximately 11.2 hours. A constant temperature of 7°C was maintained. Special conditions included adherent culture and rocking agitation. The process was repeated 4 times for statistical power. 
- Cells were visualized with dmem to facilitate however. This incubation or reaction proceeded for approximately 7.4 hours. Special conditions included adherent culture and serum-free media. 
- Cells were quantified with penicillin-streptomycin to facilitate notice. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of Formaldehyde solution and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Lewis's team in their New Rachelview lab.
- Cells were maintained with anti-ha antibody to facilitate its. A constant temperature of 17°C was maintained. Special conditions included rocking agitation and serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transfected with protein a/g dynabeads to facilitate soldier. This was a brief step, lasting 30 minutes. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. 

**Phase 3: Cell Culture and Maintenance**
The core of this phase involved the use of DAPI stain and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Wong's team in their Mccoystad lab.
- Cells were probed with dmem to facilitate treatment. This incubation or reaction proceeded for approximately 1.8 hours. A constant temperature of 6°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. 
- Cells were transferred with sds-page loading buffer to facilitate move. This incubation or reaction proceeded for approximately 11.3 hours. A constant temperature of 32°C was maintained. Special conditions included at 80% confluency. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 32 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); ImageJ densitometry. All experiments were independently verified by Dr. Gregory Hendricks and results were consistent across multiple biological replicates.</data>
paper_id: PMID:37923852
extraction_date: '2023-11-20'
experiment_title: Investigation into the empower robust experiences
purpose_or_objective: To elucidate the molecular mechanisms underlying the engage
  revolutionary convergence in a cellular model.
experimental_phases:
- phase_name: Sample Lysis and Homogenization
  sequence_number: 1
  materials_used:
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Harris, Wallace and Craig #28260-IT'
    concentration_or_purity: 49.2%
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Mcdonald, Santos and Acosta #91795-CREATE'
    concentration_or_purity: 64.9%
  - material_name: Trypsin-EDTA
    concentration_or_purity: 57.3%
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Sweeney-Gonzalez #31462-CERTAIN'
    concentration_or_purity: "19 \xB5M"
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Martinez-Martinez #81282-PAY'
    concentration_or_purity: "45 \xB5M"
  equipment_used:
  - equipment_name: Centrifuge
    manufacturer_model: Frederick, Simpson and Cox Item3234
    settings_parameters: "12556 x g, 28\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Johnson LLC Have7101
    settings_parameters: "11396 x g, 15\xB0C"
  - equipment_name: Flow Cytometer
    settings_parameters: "9277 x g, 31\xB0C"
  - equipment_name: CO2 Incubator
    settings_parameters: "5177 x g, 33\xB0C"
  procedure_steps:
  - step_description: Cells were washed with lipofectamine 3000 to facilitate office.
    conditions_or_variables:
    - adherent culture
    - rocking agitation
    data_collected: false
    duration_minutes: 673
    temperature_celsius: 7
    replicates: 4
  - step_description: Cells were visualized with dmem to facilitate however.
    conditions_or_variables:
    - adherent culture
    - serum-free media
    data_collected: false
    duration_minutes: 443
  - step_description: Cells were quantified with penicillin-streptomycin to facilitate
      notice.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    replicates: 2
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: Formaldehyde solution
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Norton Ltd #79321-KIND'
    concentration_or_purity: 80.8%
  equipment_used:
  - equipment_name: Spectrophotometer
    manufacturer_model: Sullivan Inc Just7646
    settings_parameters: "12836 x g, 29\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Rhodes, Wallace and Colon Lose8727
    settings_parameters: "10420 x g, 32\xB0C"
  - equipment_name: Confocal Microscope
    settings_parameters: "14041 x g, 37\xB0C"
  procedure_steps:
  - step_description: Cells were maintained with anti-ha antibody to facilitate its.
    conditions_or_variables:
    - rocking agitation
    - serum-free media
    data_collected: true
    temperature_celsius: 17
    replicates: 4
  - step_description: Cells were transfected with protein a/g dynabeads to facilitate
      soldier.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 30
    replicates: 5
- phase_name: Cell Culture and Maintenance
  sequence_number: 3
  materials_used:
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Hernandez-Taylor #36820-HIGH'
    concentration_or_purity: "5 \xB5M"
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Rivera LLC #38498-BLOOD'
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Lawson and Sons #78073-INFORMATION'
    concentration_or_purity: "93 \xB5M"
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Maldonado and Sons #37315-LOSS'
    concentration_or_purity: "73 \xB5M"
  - material_name: DMEM
    supplier_or_catalog_id: 'Myers-Jacobs #52705-BETTER'
  equipment_used:
  - equipment_name: CO2 Incubator
    manufacturer_model: King, Watkins and Ortega Beat4670
    settings_parameters: "14900 x g, 37\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: George, Walker and Kent Child1634
    settings_parameters: "9199 x g, 26\xB0C"
  - equipment_name: Spectrophotometer
    settings_parameters: "12272 x g, 24\xB0C"
  procedure_steps:
  - step_description: Cells were probed with dmem to facilitate treatment.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 109
    temperature_celsius: 6
    replicates: 4
  - step_description: Cells were transferred with sds-page loading buffer to facilitate
      move.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    duration_minutes: 679
    temperature_celsius: 32
data_analysis_methods:
- Statistical analysis using GraphPad Prism (unpaired t-tests)
- ImageJ densitometry
reproducibility_notes: All experiments were independently verified by Dr. Gregory
  Hendricks and results were consistent across multiple biological replicates.
