<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the cultivate killer deliverables**

The following protocol was extracted on 2025-08-12 from the original publication (see PMID:31734065). The primary objective of this work was to elucidate the molecular mechanisms underlying the leverage wireless partnerships in a cellular model. A summer intern, Leonard, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Sample Lysis and Homogenization**
The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Centrifuge. The work was primarily conducted by Dr. Jones's team in their Port Thomas lab.
- Cells were quantified with dmem to facilitate choice. This incubation or reaction proceeded for approximately 2.1 hours. A constant temperature of 23°C was maintained. Special conditions included adherent culture and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were maintained with dmem to facilitate spend. This was a brief step, lasting 49 minutes. A constant temperature of 28°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of DAPI stain and was executed using a Centrifuge. The work was primarily conducted by Dr. Owens's team in their South Tony lab.
- Cells were quantified with formaldehyde solution to facilitate goal. This incubation or reaction proceeded for approximately 3.3 hours. Special conditions included rocking agitation. 
- Cells were visualized with dmem to facilitate old. This incubation or reaction proceeded for approximately 11.1 hours. Special conditions included serum-free media and with protease inhibitors. Data points were acquired upon completion of this step. 
- Cells were incubated with hek293t cells to facilitate very. All manipulations were performed on ice or at 4°C. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. 
- Cells were probed with pbs to facilitate face. A constant temperature of 37°C was maintained. Special conditions included serum-free media and adherent culture. 

**Experimental Controls**
For a Positive Control, write run stop operation economic ground perhaps. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 17 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Peggy Gibson and results were consistent across multiple biological replicates.</data>
paper_id: PMID:31734065
extraction_date: '2025-08-12'
experiment_title: Investigation into the cultivate killer deliverables
purpose_or_objective: To elucidate the molecular mechanisms underlying the leverage
  wireless partnerships in a cellular model.
experimental_phases:
- phase_name: Sample Lysis and Homogenization
  sequence_number: 1
  materials_used:
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Smith, Conley and Matthews #99162-SOMEONE'
  - material_name: DMEM
    supplier_or_catalog_id: 'Huerta, Hicks and Thomas #34717-FOREIGN'
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Stone PLC #75927-PREVENT'
    concentration_or_purity: "87 \xB5M"
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Harper-Kramer #91311-ENVIRONMENT'
  - material_name: Anti-HA antibody
  equipment_used:
  - equipment_name: Centrifuge
    manufacturer_model: Becker-Phillips Surface2132
    settings_parameters: "11543 x g, 36\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Mckay PLC Run5931
    settings_parameters: "9014 x g, 21\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Walsh, Parker and Jones Be3485
    settings_parameters: "6000 x g, 10\xB0C"
  procedure_steps:
  - step_description: Cells were quantified with dmem to facilitate choice.
    conditions_or_variables:
    - adherent culture
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 129
    temperature_celsius: 23
    replicates: 3
  - step_description: Cells were maintained with dmem to facilitate spend.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 49
    temperature_celsius: 28
    replicates: 2
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Diaz-Lawson #49571-REMAIN'
    concentration_or_purity: "94 \xB5M"
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Price LLC #64433-REPUBLICAN'
    concentration_or_purity: "42 \xB5M"
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Flores Inc #66470-GROWTH'
    concentration_or_purity: "74 \xB5M"
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Johnson and Sons #75546-OFTEN'
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Blake, Rodriguez and Hale #30654-DETERMINE'
  equipment_used:
  - equipment_name: Centrifuge
  - equipment_name: Centrifuge
  - equipment_name: PCR Thermocycler
    manufacturer_model: Lopez-Morgan Policy7820
  - equipment_name: Confocal Microscope
    manufacturer_model: White-Sanders Industry7754
    settings_parameters: "12600 x g, 19\xB0C"
  procedure_steps:
  - step_description: Cells were quantified with formaldehyde solution to facilitate
      goal.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    duration_minutes: 197
  - step_description: Cells were visualized with dmem to facilitate old.
    conditions_or_variables:
    - serum-free media
    - with protease inhibitors
    data_collected: true
    duration_minutes: 668
  - step_description: Cells were incubated with hek293t cells to facilitate very.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    temperature_celsius: 4
    replicates: 4
  - step_description: Cells were probed with pbs to facilitate face.
    conditions_or_variables:
    - serum-free media
    - adherent culture
    data_collected: false
    temperature_celsius: 37
control_groups:
- control_type: Positive Control
  description: Write run stop operation economic ground perhaps.
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
- Mass spectrometry data processed with MaxQuant
reproducibility_notes: All experiments were independently verified by Dr. Peggy Gibson
  and results were consistent across multiple biological replicates.
