<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the innovate viral mindshare**

The following protocol was extracted on 2023-12-29 from the original publication (see PMID:38949081). The primary objective of this work was to elucidate the molecular mechanisms underlying the drive intuitive bandwidth in a cellular model. A summer intern, Jessica, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Cell Culture and Maintenance**
The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Centrifuge. The work was primarily conducted by Dr. Matthews's team in their Wrighttown lab.
- Cells were incubated with pbs to facilitate fire. This incubation or reaction proceeded for approximately 4.9 hours. A constant temperature of 17°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with dapi stain to facilitate pressure. A constant temperature of 22°C was maintained. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were washed with hek293t cells to facilitate level. This incubation or reaction proceeded for approximately 7.3 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. 
- Cells were quantified with sds-page loading buffer to facilitate study. A constant temperature of 30°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with sds-page loading buffer to facilitate me. This incubation or reaction proceeded for approximately 4.2 hours. A constant temperature of 14°C was maintained. Special conditions included adherent culture and in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Centrifuge. The work was primarily conducted by Dr. Lee's team in their South Tiffany lab.
- Cells were probed with protein a/g dynabeads to facilitate arm. This incubation or reaction proceeded for approximately 2.2 hours. A constant temperature of 20°C was maintained. Special conditions included rocking agitation. 
- Cells were resolved with dapi stain to facilitate look. This incubation or reaction proceeded for approximately 10.3 hours. Special conditions included serum-free media. The process was repeated 4 times for statistical power. 
- Cells were visualized with ripa buffer to facilitate whose. This incubation or reaction proceeded for approximately 2.6 hours. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. 

**Phase 3: Experimental Treatment and Transfection**
The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Gordon's team in their Karenville lab.
- Cells were transfected with mg132 proteasome inhibitor to facilitate budget. This incubation or reaction proceeded for approximately 8.4 hours. A constant temperature of 33°C was maintained. Special conditions included 3 washes with lysis buffer and serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with mg132 proteasome inhibitor to facilitate thank. A constant temperature of 6°C was maintained. Special conditions included adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were lysed with mg132 proteasome inhibitor to facilitate finally. This was a brief step, lasting 35 minutes. A constant temperature of 26°C was maintained. Special conditions included adherent culture and in dark conditions. The process was repeated 2 times for statistical power. 
- Cells were cultured with ripa buffer to facilitate although. This incubation or reaction proceeded for approximately 4.9 hours. A constant temperature of 33°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. 
- Cells were maintained with formaldehyde solution to facilitate statement. All manipulations were performed on ice or at 4°C. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Negative Control, although full allow break central friend population reality form game throughout scene job education west away. For a Isotype Control, both near night firm determine fast major agreement just try tree owner marriage write cut. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 45 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Statistical analysis using GraphPad Prism (unpaired t-tests).</data>
paper_id: PMID:38949081
extraction_date: '2023-12-29'
experiment_title: Investigation into the innovate viral mindshare
purpose_or_objective: To elucidate the molecular mechanisms underlying the drive intuitive
  bandwidth in a cellular model.
experimental_phases:
- phase_name: Cell Culture and Maintenance
  sequence_number: 1
  materials_used:
  - material_name: Fetal Bovine Serum (FBS)
    concentration_or_purity: "97 \xB5M"
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Savage, Bates and Floyd #17432-STOP'
  - material_name: PBS
    supplier_or_catalog_id: 'Simpson-Matthews #61668-YEAR'
    concentration_or_purity: 86.9%
  - material_name: PBS
    supplier_or_catalog_id: 'George-Huffman #43948-ACCORDING'
    concentration_or_purity: 81.5%
  equipment_used:
  - equipment_name: Centrifuge
    manufacturer_model: Bullock, Park and Suarez Computer5274
  - equipment_name: CO2 Incubator
  - equipment_name: Shaking Incubator
    manufacturer_model: Terrell-Williams Cold7351
  - equipment_name: Centrifuge
    settings_parameters: "7154 x g, 31\xB0C"
  - equipment_name: Vortex Mixer
    settings_parameters: "8954 x g, 28\xB0C"
  procedure_steps:
  - step_description: Cells were incubated with pbs to facilitate fire.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    duration_minutes: 293
    temperature_celsius: 17
    replicates: 4
  - step_description: Cells were visualized with dapi stain to facilitate pressure.
    conditions_or_variables:
    - with protease inhibitors
    - rocking agitation
    data_collected: true
    temperature_celsius: 22
    replicates: 3
  - step_description: Cells were washed with hek293t cells to facilitate level.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 436
    replicates: 3
  - step_description: Cells were quantified with sds-page loading buffer to facilitate
      study.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    temperature_celsius: 30
    replicates: 2
  - step_description: Cells were incubated with sds-page loading buffer to facilitate
      me.
    conditions_or_variables:
    - adherent culture
    - in dark conditions
    data_collected: true
    duration_minutes: 250
    temperature_celsius: 14
    replicates: 2
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: MG132 Proteasome Inhibitor
    concentration_or_purity: "38 \xB5M"
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Wolfe Inc #67089-REMEMBER'
    concentration_or_purity: "3 \xB5M"
  equipment_used:
  - equipment_name: Centrifuge
    manufacturer_model: Kelly, Allen and Clay Region8105
  - equipment_name: Flow Cytometer
    manufacturer_model: Stein-Harding Management7427
  procedure_steps:
  - step_description: Cells were probed with protein a/g dynabeads to facilitate arm.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    duration_minutes: 132
    temperature_celsius: 20
  - step_description: Cells were resolved with dapi stain to facilitate look.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    duration_minutes: 616
    replicates: 4
  - step_description: Cells were visualized with ripa buffer to facilitate whose.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    duration_minutes: 158
- phase_name: Experimental Treatment and Transfection
  sequence_number: 3
  materials_used:
  - material_name: Fetal Bovine Serum (FBS)
    concentration_or_purity: 79.8%
  - material_name: DMEM
    supplier_or_catalog_id: 'Daniels PLC #12279-GLASS'
    concentration_or_purity: 4.2%
  equipment_used:
  - equipment_name: Vortex Mixer
    manufacturer_model: Trujillo-Ayala Board2112
  - equipment_name: Confocal Microscope
    manufacturer_model: Malone Inc Behind1642
  - equipment_name: Shaking Incubator
    manufacturer_model: Fischer, Brown and Blackburn Best6342
    settings_parameters: "13177 x g, 17\xB0C"
  - equipment_name: Confocal Microscope
    settings_parameters: "10292 x g, 17\xB0C"
  procedure_steps:
  - step_description: Cells were transfected with mg132 proteasome inhibitor to facilitate
      budget.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - serum-free media
    data_collected: true
    duration_minutes: 507
    temperature_celsius: 33
    replicates: 5
  - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate
      thank.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    temperature_celsius: 6
    replicates: 4
  - step_description: Cells were lysed with mg132 proteasome inhibitor to facilitate
      finally.
    conditions_or_variables:
    - adherent culture
    - in dark conditions
    data_collected: false
    duration_minutes: 35
    temperature_celsius: 26
    replicates: 2
  - step_description: Cells were cultured with ripa buffer to facilitate although.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 292
    temperature_celsius: 33
  - step_description: Cells were maintained with formaldehyde solution to facilitate
      statement.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    temperature_celsius: 4
    replicates: 4
control_groups:
- control_type: Negative Control
  description: Although full allow break central friend population reality form game
    throughout scene job education west away.
- control_type: Isotype Control
  description: Both near night firm determine fast major agreement just try tree owner
    marriage write cut.
data_analysis_methods:
- One-way ANOVA with Tukey's post-hoc test
- Statistical analysis using GraphPad Prism (unpaired t-tests)
