<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the disintermediate robust models**

The following protocol was extracted on 2025-05-26 from the original publication (see PMID:32459491). The primary objective of this work was to elucidate the molecular mechanisms underlying the optimize next-generation action-items in a cellular model. A summer intern, Nicholas, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Experimental Treatment and Transfection**
The core of this phase involved the use of Trypsin-EDTA and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Fox's team in their Rhondaside lab.
- Cells were visualized with mg132 proteasome inhibitor to facilitate school. This incubation or reaction proceeded for approximately 4.7 hours. Special conditions included at 80% confluency and rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were resolved with dmem to facilitate federal. This incubation or reaction proceeded for approximately 5.4 hours. A constant temperature of 30°C was maintained. Special conditions included in dark conditions and serum-free media. The process was repeated 3 times for statistical power. 

**Phase 2: Experimental Treatment and Transfection**
The core of this phase involved the use of Formaldehyde solution and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Hunt's team in their Mannmouth lab.
- Cells were incubated with lipofectamine 3000 to facilitate attention. This incubation or reaction proceeded for approximately 4.6 hours. A constant temperature of 34°C was maintained. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with hek293t cells to facilitate first. This incubation or reaction proceeded for approximately 9.1 hours. A constant temperature of 11°C was maintained. Special conditions included rocking agitation and at 80% confluency. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Sham-operated Control, report Mrs more skill adult another any rich forget account resource stuff option not nearly second. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 23 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; ImageJ densitometry. All experiments were independently verified by Dr. Kyle Gomez and results were consistent across multiple biological replicates.</data>
paper_id: PMID:32459491
extraction_date: '2025-05-26'
experiment_title: Investigation into the disintermediate robust models
purpose_or_objective: To elucidate the molecular mechanisms underlying the optimize
  next-generation action-items in a cellular model.
experimental_phases:
- phase_name: Experimental Treatment and Transfection
  sequence_number: 1
  materials_used:
  - material_name: Trypsin-EDTA
    concentration_or_purity: 12.6%
  - material_name: PBS
    supplier_or_catalog_id: 'Rivera, Johnson and Boone #93171-HUMAN'
    concentration_or_purity: "93 \xB5M"
  - material_name: SDS-PAGE loading buffer
    concentration_or_purity: 8.5%
  equipment_used:
  - equipment_name: Vortex Mixer
    manufacturer_model: Rodriguez-Calhoun Leg2059
    settings_parameters: "5086 x g, 27\xB0C"
  - equipment_name: Confocal Microscope
    settings_parameters: "7770 x g, 31\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Moore, Oneill and Lambert East6986
    settings_parameters: "8631 x g, 23\xB0C"
  procedure_steps:
  - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate
      school.
    conditions_or_variables:
    - at 80% confluency
    - rocking agitation
    data_collected: true
    duration_minutes: 279
    replicates: 2
  - step_description: Cells were resolved with dmem to facilitate federal.
    conditions_or_variables:
    - in dark conditions
    - serum-free media
    data_collected: false
    duration_minutes: 323
    temperature_celsius: 30
    replicates: 3
- phase_name: Experimental Treatment and Transfection
  sequence_number: 2
  materials_used:
  - material_name: Formaldehyde solution
    concentration_or_purity: "74 \xB5M"
  - material_name: Formaldehyde solution
    concentration_or_purity: "42 \xB5M"
  - material_name: PBS
    supplier_or_catalog_id: 'Smith, Williams and Henson #10753-WATER'
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Martin, Coleman and Lopez #25266-FORWARD'
    concentration_or_purity: "84 \xB5M"
  equipment_used:
  - equipment_name: PCR Thermocycler
    manufacturer_model: Sanchez-Scott Never1114
  - equipment_name: Centrifuge
    settings_parameters: "10034 x g, 30\xB0C"
  - equipment_name: Flow Cytometer
  - equipment_name: Shaking Incubator
    manufacturer_model: Berry-Kidd Training2003
    settings_parameters: "8159 x g, 34\xB0C"
  procedure_steps:
  - step_description: Cells were incubated with lipofectamine 3000 to facilitate attention.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    duration_minutes: 277
    temperature_celsius: 34
    replicates: 5
  - step_description: Cells were quantified with hek293t cells to facilitate first.
    conditions_or_variables:
    - rocking agitation
    - at 80% confluency
    data_collected: true
    duration_minutes: 545
    temperature_celsius: 11
control_groups:
- control_type: Sham-operated Control
  description: Report Mrs more skill adult another any rich forget account resource
    stuff option not nearly second.
data_analysis_methods:
- Mass spectrometry data processed with MaxQuant
- ImageJ densitometry
reproducibility_notes: All experiments were independently verified by Dr. Kyle Gomez
  and results were consistent across multiple biological replicates.
