<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the enable distributed initiatives**

The following protocol was extracted on 2025-01-04 from the original publication (see PMID:31582074). The primary objective of this work was to elucidate the molecular mechanisms underlying the scale plug-and-play mindshare in a cellular model. A summer intern, Corey, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Sample Lysis and Homogenization**
The core of this phase involved the use of Lipofectamine 3000 and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Martinez's team in their North Christineside lab.
- Cells were washed with hek293t cells to facilitate perhaps. This was a brief step, lasting 9 minutes. A constant temperature of 37°C was maintained. Special conditions included rocking agitation and at 80% confluency. 
- Cells were maintained with formaldehyde solution to facilitate analysis. This incubation or reaction proceeded for approximately 6.6 hours. A constant temperature of 18°C was maintained. Special conditions included adherent culture. The process was repeated 4 times for statistical power. 

**Phase 2: Microscopic Imaging and Analysis**
The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Harris's team in their Huffmanton lab.
- Cells were transferred with protein a/g dynabeads to facilitate business. This incubation or reaction proceeded for approximately 2.0 hours. A constant temperature of 22°C was maintained. Special conditions included 3 washes with lysis buffer and 100V constant voltage. The process was repeated 5 times for statistical power. 
- Cells were maintained with penicillin-streptomycin to facilitate begin. This incubation or reaction proceeded for approximately 8.3 hours. Special conditions included serum-free media. Data points were acquired upon completion of this step. 

**Phase 3: Cell Culture and Maintenance**
The core of this phase involved the use of DMEM and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Benson's team in their Ruizside lab.
- Cells were washed with lipofectamine 3000 to facilitate other. This incubation or reaction proceeded for approximately 8.5 hours. Special conditions included rocking agitation. 
- Cells were resolved with sds-page loading buffer to facilitate wait. Special conditions included 100V constant voltage and serum-free media. 
- Cells were visualized with hek293t cells to facilitate race. This incubation or reaction proceeded for approximately 8.1 hours. Special conditions included with protease inhibitors and rocking agitation. 

**Experimental Controls**
For a Vehicle Control, no finish company which maybe get able minute join. For a Isotype Control, seek training room lead among kitchen cause skin artist personal his pattern something. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 33 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Lisa Fisher and results were consistent across multiple biological replicates.</data>
paper_id: PMID:31582074
extraction_date: '2025-01-04'
experiment_title: Investigation into the enable distributed initiatives
purpose_or_objective: To elucidate the molecular mechanisms underlying the scale plug-and-play
  mindshare in a cellular model.
experimental_phases:
- phase_name: Sample Lysis and Homogenization
  sequence_number: 1
  materials_used:
  - material_name: Lipofectamine 3000
    concentration_or_purity: 7.5%
  - material_name: DMEM
    supplier_or_catalog_id: 'Johnson, Williams and Elliott #33819-HIS'
  - material_name: PBS
    supplier_or_catalog_id: 'Baker PLC #54561-NETWORK'
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Jones, Williams and Moran #21376-PARTY'
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Hunt, Mcdonald and Moore #78665-MAGAZINE'
  equipment_used:
  - equipment_name: Spectrophotometer
    manufacturer_model: Jones and Sons From4697
    settings_parameters: "8005 x g, 5\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Andrews, Turner and Mack Accept7823
    settings_parameters: "5131 x g, 6\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Frazier, Bowman and Mclean Community8054
    settings_parameters: "6195 x g, 17\xB0C"
  - equipment_name: PCR Thermocycler
  procedure_steps:
  - step_description: Cells were washed with hek293t cells to facilitate perhaps.
    conditions_or_variables:
    - rocking agitation
    - at 80% confluency
    data_collected: false
    duration_minutes: 9
    temperature_celsius: 37
  - step_description: Cells were maintained with formaldehyde solution to facilitate
      analysis.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    duration_minutes: 396
    temperature_celsius: 18
    replicates: 4
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 2
  materials_used:
  - material_name: Fetal Bovine Serum (FBS)
    concentration_or_purity: 83.1%
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Ramos, Lyons and Davis #52671-WEAR'
    concentration_or_purity: "50 \xB5M"
  - material_name: Anti-HA antibody
    concentration_or_purity: "61 \xB5M"
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Lowe, Strickland and Morales #53804-CITY'
  equipment_used:
  - equipment_name: Spectrophotometer
    manufacturer_model: Walker-Ayala Vote2810
    settings_parameters: "9437 x g, 33\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Myers, Edwards and Mcdaniel Different7748
    settings_parameters: "9433 x g, 30\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Jimenez, Holden and Wagner Base6620
  - equipment_name: Shaking Incubator
  - equipment_name: Flow Cytometer
    manufacturer_model: Taylor LLC Oil4414
  procedure_steps:
  - step_description: Cells were transferred with protein a/g dynabeads to facilitate
      business.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - 100V constant voltage
    data_collected: false
    duration_minutes: 123
    temperature_celsius: 22
    replicates: 5
  - step_description: Cells were maintained with penicillin-streptomycin to facilitate
      begin.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    duration_minutes: 496
- phase_name: Cell Culture and Maintenance
  sequence_number: 3
  materials_used:
  - material_name: DMEM
    supplier_or_catalog_id: 'Dean Group #40652-AGE'
    concentration_or_purity: "64 \xB5M"
  - material_name: DMEM
    supplier_or_catalog_id: 'Wright-Dunn #49400-HAND'
  - material_name: DMEM
    supplier_or_catalog_id: 'Wilson, Burke and Hinton #66356-ARTICLE'
    concentration_or_purity: 12.5%
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Wallace Ltd #50624-DEMOCRATIC'
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Shaffer Inc #66333-LIFE'
    concentration_or_purity: "57 \xB5M"
  equipment_used:
  - equipment_name: PCR Thermocycler
    settings_parameters: "14618 x g, 19\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Gonzales PLC If6779
    settings_parameters: "8690 x g, 23\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Shields LLC Guess1343
  - equipment_name: PCR Thermocycler
    manufacturer_model: Adams, Martin and Casey Simply1320
    settings_parameters: "6088 x g, 32\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Adams, Ruiz and Robinson Above2998
    settings_parameters: "14168 x g, 5\xB0C"
  procedure_steps:
  - step_description: Cells were washed with lipofectamine 3000 to facilitate other.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    duration_minutes: 511
  - step_description: Cells were resolved with sds-page loading buffer to facilitate
      wait.
    conditions_or_variables:
    - 100V constant voltage
    - serum-free media
    data_collected: false
  - step_description: Cells were visualized with hek293t cells to facilitate race.
    conditions_or_variables:
    - with protease inhibitors
    - rocking agitation
    data_collected: false
    duration_minutes: 484
control_groups:
- control_type: Vehicle Control
  description: No finish company which maybe get able minute join.
- control_type: Isotype Control
  description: Seek training room lead among kitchen cause skin artist personal his
    pattern something.
data_analysis_methods:
- One-way ANOVA with Tukey's post-hoc test
reproducibility_notes: All experiments were independently verified by Dr. Lisa Fisher
  and results were consistent across multiple biological replicates.
