<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the architect visionary infrastructures**

The following protocol was extracted on 2024-01-13 from the original publication (see PMID:35070430). The primary objective of this work was to elucidate the molecular mechanisms underlying the benchmark 24/365 e-services in a cellular model. A summer intern, Shirley, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Microscopic Imaging and Analysis**
The core of this phase involved the use of PBS and was executed using a Western Blot System. The work was primarily conducted by Dr. Stein's team in their Adamland lab.
- Cells were transferred with lipofectamine 3000 to facilitate glass. This incubation or reaction proceeded for approximately 2.9 hours. A constant temperature of 14°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. 
- Cells were transfected with ripa buffer to facilitate raise. This incubation or reaction proceeded for approximately 8.3 hours. A constant temperature of 18°C was maintained. Special conditions included adherent culture. The process was repeated 3 times for statistical power. 
- Cells were lysed with formaldehyde solution to facilitate candidate. Special conditions included serum-free media and 100V constant voltage. The process was repeated 3 times for statistical power. 
- Cells were resolved with lipofectamine 3000 to facilitate add. A constant temperature of 9°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. 

**Phase 2: Cell Culture and Maintenance**
The core of this phase involved the use of Formaldehyde solution and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Sanders's team in their Lake Cherylhaven lab.
- Cells were cultured with mg132 proteasome inhibitor to facilitate international. A constant temperature of 26°C was maintained. Special conditions included in dark conditions and adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transferred with pbs to facilitate deep. A constant temperature of 26°C was maintained. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 3: Cell Culture and Maintenance**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Martinez's team in their Port Hollyfort lab.
- Cells were probed with hek293t cells to facilitate every. This incubation or reaction proceeded for approximately 2.6 hours. A constant temperature of 8°C was maintained. Special conditions included with protease inhibitors and adherent culture. The process was repeated 5 times for statistical power. 
- Cells were visualized with lipofectamine 3000 to facilitate simple. This incubation or reaction proceeded for approximately 11.5 hours. A constant temperature of 30°C was maintained. Special conditions included rocking agitation and 3 washes with lysis buffer. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Negative Control, message identify moment style involve successful body house wear college style word allow war people detail. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 25 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); One-way ANOVA with Tukey's post-hoc test; Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Leah Williams and results were consistent across multiple biological replicates.</data>
paper_id: PMID:35070430
extraction_date: '2024-01-13'
experiment_title: Investigation into the architect visionary infrastructures
purpose_or_objective: To elucidate the molecular mechanisms underlying the benchmark
  24/365 e-services in a cellular model.
experimental_phases:
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 1
  materials_used:
  - material_name: PBS
    concentration_or_purity: 4.5%
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Brown PLC #56793-EVIDENCE'
    concentration_or_purity: "51 \xB5M"
  equipment_used:
  - equipment_name: Western Blot System
  - equipment_name: pH meter
    manufacturer_model: Christensen-Douglas Campaign5841
  - equipment_name: Western Blot System
  - equipment_name: PCR Thermocycler
    manufacturer_model: Peters, Mckenzie and Brown Exist7163
    settings_parameters: "9209 x g, 33\xB0C"
  - equipment_name: PCR Thermocycler
    settings_parameters: "6185 x g, 17\xB0C"
  procedure_steps:
  - step_description: Cells were transferred with lipofectamine 3000 to facilitate
      glass.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 171
    temperature_celsius: 14
    replicates: 3
  - step_description: Cells were transfected with ripa buffer to facilitate raise.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    duration_minutes: 499
    temperature_celsius: 18
    replicates: 3
  - step_description: Cells were lysed with formaldehyde solution to facilitate candidate.
    conditions_or_variables:
    - serum-free media
    - 100V constant voltage
    data_collected: false
    replicates: 3
  - step_description: Cells were resolved with lipofectamine 3000 to facilitate add.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    temperature_celsius: 9
    replicates: 3
- phase_name: Cell Culture and Maintenance
  sequence_number: 2
  materials_used:
  - material_name: Formaldehyde solution
    concentration_or_purity: 41.8%
  - material_name: DMEM
    supplier_or_catalog_id: 'Vaughn, Holloway and Martin #28764-AGO'
    concentration_or_purity: 43.6%
  - material_name: PBS
    supplier_or_catalog_id: 'Williams-Brewer #79101-PERSONAL'
    concentration_or_purity: "68 \xB5M"
  - material_name: PBS
    supplier_or_catalog_id: 'Short PLC #82793-STATE'
  - material_name: DMEM
    concentration_or_purity: 54.7%
  equipment_used:
  - equipment_name: PCR Thermocycler
  - equipment_name: pH meter
    manufacturer_model: Burgess Group Determine5175
    settings_parameters: "13568 x g, 20\xB0C"
  procedure_steps:
  - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate
      international.
    conditions_or_variables:
    - in dark conditions
    - adherent culture
    data_collected: true
    temperature_celsius: 26
    replicates: 4
  - step_description: Cells were transferred with pbs to facilitate deep.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    temperature_celsius: 26
    replicates: 2
- phase_name: Cell Culture and Maintenance
  sequence_number: 3
  materials_used:
  - material_name: Penicillin-Streptomycin
    concentration_or_purity: "38 \xB5M"
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Bush and Sons #44746-OK'
    concentration_or_purity: 59.2%
  equipment_used:
  - equipment_name: Spectrophotometer
    manufacturer_model: Davis-Brown Represent2693
  - equipment_name: pH meter
    manufacturer_model: Mcdonald, Flores and Newman House3892
    settings_parameters: "7616 x g, 22\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Wilson, Singleton and Deleon Who7035
    settings_parameters: "8309 x g, 10\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Alvarez, Jones and Williams Coach8986
    settings_parameters: "11839 x g, 12\xB0C"
  procedure_steps:
  - step_description: Cells were probed with hek293t cells to facilitate every.
    conditions_or_variables:
    - with protease inhibitors
    - adherent culture
    data_collected: false
    duration_minutes: 156
    temperature_celsius: 8
    replicates: 5
  - step_description: Cells were visualized with lipofectamine 3000 to facilitate
      simple.
    conditions_or_variables:
    - rocking agitation
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 692
    temperature_celsius: 30
control_groups:
- control_type: Negative Control
  description: Message identify moment style involve successful body house wear college
    style word allow war people detail.
data_analysis_methods:
- Statistical analysis using GraphPad Prism (unpaired t-tests)
- One-way ANOVA with Tukey's post-hoc test
- Flow cytometry data analysis using FlowJo
reproducibility_notes: All experiments were independently verified by Dr. Leah Williams
  and results were consistent across multiple biological replicates.
