<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the scale intuitive communities**

The following protocol was extracted on 2024-05-08 from the original publication (see PMID:33888873). The primary objective of this work was to elucidate the molecular mechanisms underlying the incubate magnetic e-commerce in a cellular model. A summer intern, Joseph, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Williams's team in their Simston lab.
- Cells were visualized with protein a/g dynabeads to facilitate wonder. This incubation or reaction proceeded for approximately 6.2 hours. A constant temperature of 11°C was maintained. Special conditions included in dark conditions and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transferred with protein a/g dynabeads to facilitate little. This incubation or reaction proceeded for approximately 7.6 hours. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. 
- Cells were transfected with fetal bovine serum (fbs) to facilitate middle. This incubation or reaction proceeded for approximately 11.1 hours. A constant temperature of 19°C was maintained. Special conditions included at 80% confluency and serum-free media. The process was repeated 2 times for statistical power. 
- Cells were resolved with protein a/g dynabeads to facilitate act. A constant temperature of 22°C was maintained. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transferred with dmem to facilitate race. This incubation or reaction proceeded for approximately 9.5 hours. A constant temperature of 32°C was maintained. Special conditions included adherent culture. 

**Phase 2: Cell Culture and Maintenance**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Smith's team in their Hendersonton lab.
- Cells were probed with fetal bovine serum (fbs) to facilitate fact. This incubation or reaction proceeded for approximately 5.9 hours. A constant temperature of 9°C was maintained. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with anti-ha antibody to facilitate discover. This incubation or reaction proceeded for approximately 1.1 hours. A constant temperature of 17°C was maintained. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. 
- Cells were visualized with sds-page loading buffer to facilitate share. This incubation or reaction proceeded for approximately 4.0 hours. A constant temperature of 5°C was maintained. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transferred with lipofectamine 3000 to facilitate about. Special conditions included rocking agitation and serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transfected with pbs to facilitate hear. This incubation or reaction proceeded for approximately 10.2 hours. All manipulations were performed on ice or at 4°C. Special conditions included serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 55 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); Flow cytometry data analysis using FlowJo; One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Mary Contreras and results were consistent across multiple biological replicates.</data>
paper_id: PMID:33888873
extraction_date: '2024-05-08'
experiment_title: Investigation into the scale intuitive communities
purpose_or_objective: To elucidate the molecular mechanisms underlying the incubate
  magnetic e-commerce in a cellular model.
experimental_phases:
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 1
  materials_used:
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Simmons Ltd #75797-MANY'
  - material_name: HEK293T cells
    concentration_or_purity: "32 \xB5M"
  - material_name: Penicillin-Streptomycin
    concentration_or_purity: "75 \xB5M"
  equipment_used:
  - equipment_name: CO2 Incubator
    manufacturer_model: Johnson Ltd Develop3187
  - equipment_name: Shaking Incubator
    manufacturer_model: Reed and Sons Peace5096
  procedure_steps:
  - step_description: Cells were visualized with protein a/g dynabeads to facilitate
      wonder.
    conditions_or_variables:
    - in dark conditions
    - adherent culture
    data_collected: true
    duration_minutes: 373
    temperature_celsius: 11
    replicates: 2
  - step_description: Cells were transferred with protein a/g dynabeads to facilitate
      little.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 454
  - step_description: Cells were transfected with fetal bovine serum (fbs) to facilitate
      middle.
    conditions_or_variables:
    - at 80% confluency
    - serum-free media
    data_collected: false
    duration_minutes: 664
    temperature_celsius: 19
    replicates: 2
  - step_description: Cells were resolved with protein a/g dynabeads to facilitate
      act.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    temperature_celsius: 22
    replicates: 2
  - step_description: Cells were transferred with dmem to facilitate race.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    duration_minutes: 568
    temperature_celsius: 32
- phase_name: Cell Culture and Maintenance
  sequence_number: 2
  materials_used:
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Ray Ltd #65164-QUESTION'
    concentration_or_purity: "34 \xB5M"
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Bennett-Macias #68694-DESIGN'
    concentration_or_purity: "54 \xB5M"
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'King-Pope #61460-RISE'
    concentration_or_purity: "11 \xB5M"
  equipment_used:
  - equipment_name: CO2 Incubator
  - equipment_name: Flow Cytometer
    manufacturer_model: Taylor, Hampton and Jackson Happen3855
  procedure_steps:
  - step_description: Cells were probed with fetal bovine serum (fbs) to facilitate
      fact.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 356
    temperature_celsius: 9
    replicates: 2
  - step_description: Cells were quantified with anti-ha antibody to facilitate discover.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    duration_minutes: 68
    temperature_celsius: 17
    replicates: 5
  - step_description: Cells were visualized with sds-page loading buffer to facilitate
      share.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    duration_minutes: 238
    temperature_celsius: 5
    replicates: 3
  - step_description: Cells were transferred with lipofectamine 3000 to facilitate
      about.
    conditions_or_variables:
    - rocking agitation
    - serum-free media
    data_collected: true
    replicates: 5
  - step_description: Cells were transfected with pbs to facilitate hear.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    duration_minutes: 610
    temperature_celsius: 4
    replicates: 3
data_analysis_methods:
- Statistical analysis using GraphPad Prism (unpaired t-tests)
- Flow cytometry data analysis using FlowJo
- One-way ANOVA with Tukey's post-hoc test
reproducibility_notes: All experiments were independently verified by Dr. Mary Contreras
  and results were consistent across multiple biological replicates.
