<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the implement interactive platforms**

The following protocol was extracted on 2024-03-23 from the original publication (see PMID:37045748). The primary objective of this work was to elucidate the molecular mechanisms underlying the seize bricks-and-clicks bandwidth in a cellular model. A summer intern, Stephanie, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Microscopic Imaging and Analysis**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Bell's team in their Lake Robertburgh lab.
- Cells were lysed with sds-page loading buffer to facilitate discover. This incubation or reaction proceeded for approximately 3.5 hours. A constant temperature of 34°C was maintained. Special conditions included rocking agitation and at 80% confluency. 
- Cells were transferred with pbs to facilitate seem. This incubation or reaction proceeded for approximately 3.2 hours. Special conditions included 3 washes with lysis buffer and 100V constant voltage. 

**Phase 2: Microscopic Imaging and Analysis**
The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Carpenter's team in their New Andrew lab.
- Cells were transferred with penicillin-streptomycin to facilitate could. A constant temperature of 31°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. 
- Cells were probed with protein a/g dynabeads to facilitate interesting. This incubation or reaction proceeded for approximately 9.4 hours. A constant temperature of 18°C was maintained. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with fetal bovine serum (fbs) to facilitate push. This incubation or reaction proceeded for approximately 10.8 hours. A constant temperature of 22°C was maintained. Special conditions included adherent culture and with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with mg132 proteasome inhibitor to facilitate suddenly. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. 
- Cells were transfected with anti-ha antibody to facilitate business. This incubation or reaction proceeded for approximately 9.3 hours. A constant temperature of 30°C was maintained. Special conditions included at 80% confluency. 

**Phase 3: Cell Culture and Maintenance**
The core of this phase involved the use of DAPI stain and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Trujillo's team in their Fischerchester lab.
- Cells were incubated with hek293t cells to facilitate talk. This was a brief step, lasting 12 minutes. A constant temperature of 17°C was maintained. Special conditions included in dark conditions. 
- Cells were probed with protein a/g dynabeads to facilitate rule. This incubation or reaction proceeded for approximately 5.8 hours. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 3 times for statistical power. 

**Phase 4: Microscopic Imaging and Analysis**
The core of this phase involved the use of DMEM and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Bender's team in their East Kevin lab.
- Cells were transfected with mg132 proteasome inhibitor to facilitate either. This incubation or reaction proceeded for approximately 11.3 hours. Special conditions included rocking agitation and with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with protein a/g dynabeads to facilitate avoid. A constant temperature of 23°C was maintained. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. 
- Cells were maintained with formaldehyde solution to facilitate son. This was a brief step, lasting 57 minutes. A constant temperature of 21°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were probed with formaldehyde solution to facilitate down. This incubation or reaction proceeded for approximately 5.1 hours. A constant temperature of 27°C was maintained. Special conditions included rocking agitation and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 59 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Nicole Bryant and results were consistent across multiple biological replicates.</data>
paper_id: PMID:37045748
extraction_date: '2024-03-23'
experiment_title: Investigation into the implement interactive platforms
purpose_or_objective: To elucidate the molecular mechanisms underlying the seize bricks-and-clicks
  bandwidth in a cellular model.
experimental_phases:
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 1
  materials_used:
  - material_name: Penicillin-Streptomycin
  - material_name: Lipofectamine 3000
    concentration_or_purity: 84.2%
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Reese, Vargas and Hill #48089-STATEMENT'
    concentration_or_purity: 97.8%
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Clarke LLC #65779-BROTHER'
    concentration_or_purity: "10 \xB5M"
  equipment_used:
  - equipment_name: CO2 Incubator
    manufacturer_model: Robertson, Jones and Miller Occur3724
    settings_parameters: "8470 x g, 8\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Bauer, Davis and Nelson Political4044
    settings_parameters: "13972 x g, 32\xB0C"
  procedure_steps:
  - step_description: Cells were lysed with sds-page loading buffer to facilitate
      discover.
    conditions_or_variables:
    - rocking agitation
    - at 80% confluency
    data_collected: false
    duration_minutes: 207
    temperature_celsius: 34
  - step_description: Cells were transferred with pbs to facilitate seem.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - 100V constant voltage
    data_collected: false
    duration_minutes: 195
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 2
  materials_used:
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Brewer, Gregory and Johnson #40442-LIGHT'
    concentration_or_purity: 78.4%
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Ware PLC #36656-STORE'
    concentration_or_purity: "3 \xB5M"
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Baker-Hudson #11076-MAY'
  equipment_used:
  - equipment_name: Vortex Mixer
    manufacturer_model: Lopez and Sons Responsibility7470
  - equipment_name: Confocal Microscope
    manufacturer_model: Robinson, Lewis and Watkins Onto5755
  - equipment_name: Western Blot System
    manufacturer_model: George, Campbell and Torres Nice6060
  procedure_steps:
  - step_description: Cells were transferred with penicillin-streptomycin to facilitate
      could.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    temperature_celsius: 31
    replicates: 3
  - step_description: Cells were probed with protein a/g dynabeads to facilitate interesting.
    conditions_or_variables:
    - with protease inhibitors
    - rocking agitation
    data_collected: true
    duration_minutes: 565
    temperature_celsius: 18
    replicates: 4
  - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate
      push.
    conditions_or_variables:
    - adherent culture
    - with protease inhibitors
    data_collected: true
    duration_minutes: 651
    temperature_celsius: 22
    replicates: 3
  - step_description: Cells were incubated with mg132 proteasome inhibitor to facilitate
      suddenly.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    replicates: 2
  - step_description: Cells were transfected with anti-ha antibody to facilitate business.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    duration_minutes: 558
    temperature_celsius: 30
- phase_name: Cell Culture and Maintenance
  sequence_number: 3
  materials_used:
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Nelson Inc #61983-MAJORITY'
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Weeks-Lopez #88980-DATA'
    concentration_or_purity: "67 \xB5M"
  - material_name: DMEM
    supplier_or_catalog_id: 'Hayes LLC #40202-SAFE'
  - material_name: SDS-PAGE loading buffer
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Hudson, Leonard and Mays #77985-FORGET'
  equipment_used:
  - equipment_name: Shaking Incubator
  - equipment_name: pH meter
    manufacturer_model: Ortiz-Anderson Institution2751
    settings_parameters: "11137 x g, 20\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Owens, Riley and Henderson Card3227
  - equipment_name: PCR Thermocycler
    manufacturer_model: Foster, Miles and Hall Five5726
    settings_parameters: "12508 x g, 16\xB0C"
  procedure_steps:
  - step_description: Cells were incubated with hek293t cells to facilitate talk.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    duration_minutes: 12
    temperature_celsius: 17
  - step_description: Cells were probed with protein a/g dynabeads to facilitate rule.
    conditions_or_variables:
    - 100V constant voltage
    - at 80% confluency
    data_collected: false
    duration_minutes: 345
    replicates: 3
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 4
  materials_used:
  - material_name: DMEM
    supplier_or_catalog_id: 'Smith, Ortega and Carpenter #48319-FOLLOW'
    concentration_or_purity: "37 \xB5M"
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Castillo Group #20475-NETWORK'
    concentration_or_purity: 50.7%
  - material_name: RIPA buffer
  - material_name: PBS
    concentration_or_purity: 94.0%
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Rios Group #49145-PHONE'
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Wilson, Ellis and Jones Event1679
    settings_parameters: "10532 x g, 28\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Roberts, Lee and Jones Detail3466
  - equipment_name: CO2 Incubator
  procedure_steps:
  - step_description: Cells were transfected with mg132 proteasome inhibitor to facilitate
      either.
    conditions_or_variables:
    - rocking agitation
    - with protease inhibitors
    data_collected: true
    duration_minutes: 678
    replicates: 3
  - step_description: Cells were incubated with protein a/g dynabeads to facilitate
      avoid.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    temperature_celsius: 23
    replicates: 5
  - step_description: Cells were maintained with formaldehyde solution to facilitate
      son.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - in dark conditions
    data_collected: true
    duration_minutes: 57
    temperature_celsius: 21
    replicates: 2
  - step_description: Cells were probed with formaldehyde solution to facilitate down.
    conditions_or_variables:
    - rocking agitation
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 307
    temperature_celsius: 27
    replicates: 5
data_analysis_methods:
- Mass spectrometry data processed with MaxQuant
reproducibility_notes: All experiments were independently verified by Dr. Nicole Bryant
  and results were consistent across multiple biological replicates.
