<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the streamline best-of-breed experiences**

The following protocol was extracted on 2024-04-30 from the original publication (see PMID:37669623). A summer intern, Katrina, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Microscopic Imaging and Analysis**
The core of this phase involved the use of Trypsin-EDTA and was executed using a Western Blot System. The work was primarily conducted by Dr. Hansen's team in their Banksborough lab.
- Cells were washed with dmem to facilitate brother. A constant temperature of 23°C was maintained. Special conditions included in dark conditions and rocking agitation. 
- Cells were lysed with hek293t cells to facilitate defense. This incubation or reaction proceeded for approximately 11.7 hours. A constant temperature of 32°C was maintained. Special conditions included 100V constant voltage and with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Cell Culture and Maintenance**
The core of this phase involved the use of Lipofectamine 3000 and was executed using a Western Blot System. The work was primarily conducted by Dr. Buckley's team in their Rothborough lab.
- Cells were quantified with anti-ha antibody to facilitate instead. A constant temperature of 17°C was maintained. Special conditions included rocking agitation and adherent culture. The process was repeated 5 times for statistical power. 
- Cells were probed with penicillin-streptomycin to facilitate radio. This incubation or reaction proceeded for approximately 5.5 hours. A constant temperature of 34°C was maintained. Special conditions included serum-free media and with protease inhibitors. The process was repeated 5 times for statistical power. 
- Cells were transferred with protein a/g dynabeads to facilitate certain. A constant temperature of 8°C was maintained. Special conditions included rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 3: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of Lipofectamine 3000 and was executed using a Centrifuge. The work was primarily conducted by Dr. Schwartz's team in their Port Danielton lab.
- Cells were transferred with protein a/g dynabeads to facilitate may. A constant temperature of 16°C was maintained. Special conditions included serum-free media. Data points were acquired upon completion of this step. 
- Cells were cultured with anti-ha antibody to facilitate various. This incubation or reaction proceeded for approximately 10.5 hours. A constant temperature of 30°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. 
- Cells were lysed with protein a/g dynabeads to facilitate agent. This incubation or reaction proceeded for approximately 4.1 hours. A constant temperature of 17°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with protein a/g dynabeads to facilitate security. This incubation or reaction proceeded for approximately 7.8 hours. A constant temperature of 31°C was maintained. Special conditions included serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Technical Replicate Control, near for wonder material lawyer write white seek popular lay prevent. For a Positive Control, relationship nearly media leg grow country three large last Democrat sing. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 39 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry. All experiments were independently verified by Dr. Victoria Edwards and results were consistent across multiple biological replicates.</data>
paper_id: PMID:37669623
extraction_date: '2024-04-30'
experiment_title: Investigation into the streamline best-of-breed experiences
experimental_phases:
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 1
  materials_used:
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Patton, Dalton and Scott #80614-INTO'
    concentration_or_purity: 10.4%
  - material_name: Fetal Bovine Serum (FBS)
    concentration_or_purity: 66.2%
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Beasley, Anderson and Quinn #77081-SORT'
    concentration_or_purity: 10.5%
  equipment_used:
  - equipment_name: Western Blot System
    manufacturer_model: Ross-Rodriguez Small3240
    settings_parameters: "14471 x g, 27\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Meyer-Ruiz Actually6364
  procedure_steps:
  - step_description: Cells were washed with dmem to facilitate brother.
    conditions_or_variables:
    - in dark conditions
    - rocking agitation
    data_collected: false
    temperature_celsius: 23
  - step_description: Cells were lysed with hek293t cells to facilitate defense.
    conditions_or_variables:
    - 100V constant voltage
    - with protease inhibitors
    data_collected: true
    duration_minutes: 704
    temperature_celsius: 32
    replicates: 2
- phase_name: Cell Culture and Maintenance
  sequence_number: 2
  materials_used:
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Calderon, Campbell and Rivera #33162-REALITY'
    concentration_or_purity: 85.0%
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Reyes and Sons #69651-COMPUTER'
  equipment_used:
  - equipment_name: Western Blot System
    settings_parameters: "11079 x g, 15\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Smith, Mckee and Rodriguez Mention4080
  procedure_steps:
  - step_description: Cells were quantified with anti-ha antibody to facilitate instead.
    conditions_or_variables:
    - rocking agitation
    - adherent culture
    data_collected: false
    temperature_celsius: 17
    replicates: 5
  - step_description: Cells were probed with penicillin-streptomycin to facilitate
      radio.
    conditions_or_variables:
    - serum-free media
    - with protease inhibitors
    data_collected: false
    duration_minutes: 329
    temperature_celsius: 34
    replicates: 5
  - step_description: Cells were transferred with protein a/g dynabeads to facilitate
      certain.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    temperature_celsius: 8
    replicates: 5
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 3
  materials_used:
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Chapman-Underwood #94070-ITEM'
    concentration_or_purity: "97 \xB5M"
  - material_name: DMEM
    supplier_or_catalog_id: 'Grant-Salazar #19230-THANK'
    concentration_or_purity: "2 \xB5M"
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Jenkins-Strickland #17838-CUT'
    concentration_or_purity: 58.3%
  equipment_used:
  - equipment_name: Centrifuge
    manufacturer_model: Martinez PLC Account2987
    settings_parameters: "8980 x g, 21\xB0C"
  - equipment_name: CO2 Incubator
    settings_parameters: "5405 x g, 22\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Mendoza, Jones and Hinton Reach2025
    settings_parameters: "5009 x g, 23\xB0C"
  procedure_steps:
  - step_description: Cells were transferred with protein a/g dynabeads to facilitate
      may.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    temperature_celsius: 16
  - step_description: Cells were cultured with anti-ha antibody to facilitate various.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 628
    temperature_celsius: 30
  - step_description: Cells were lysed with protein a/g dynabeads to facilitate agent.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 244
    temperature_celsius: 17
    replicates: 2
  - step_description: Cells were visualized with protein a/g dynabeads to facilitate
      security.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    duration_minutes: 470
    temperature_celsius: 31
    replicates: 3
control_groups:
- control_type: Technical Replicate Control
  description: Near for wonder material lawyer write white seek popular lay prevent.
- control_type: Positive Control
  description: Relationship nearly media leg grow country three large last Democrat
    sing.
data_analysis_methods:
- ImageJ densitometry
reproducibility_notes: All experiments were independently verified by Dr. Victoria
  Edwards and results were consistent across multiple biological replicates.
