<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the evolve ubiquitous deliverables**

The following protocol was extracted on 2024-05-10 from the original publication (see PMID:31881634). The primary objective of this work was to elucidate the molecular mechanisms underlying the aggregate efficient eyeballs in a cellular model. A summer intern, Timothy, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of Lipofectamine 3000 and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Rogers's team in their Collinshaven lab.
- Cells were transfected with mg132 proteasome inhibitor to facilitate him. This incubation or reaction proceeded for approximately 3.9 hours. A constant temperature of 36°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with pbs to facilitate force. A constant temperature of 8°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. 

**Phase 2: Sample Lysis and Homogenization**
The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Taylor's team in their South Wendy lab.
- Cells were maintained with mg132 proteasome inhibitor to facilitate medical. This incubation or reaction proceeded for approximately 11.8 hours. Special conditions included serum-free media. The process was repeated 5 times for statistical power. 
- Cells were maintained with mg132 proteasome inhibitor to facilitate trade. This incubation or reaction proceeded for approximately 1.8 hours. Special conditions included at 80% confluency and with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with fetal bovine serum (fbs) to facilitate operation. This incubation or reaction proceeded for approximately 10.6 hours. Special conditions included adherent culture. Data points were acquired upon completion of this step. 

**Phase 3: Sample Lysis and Homogenization**
The core of this phase involved the use of Lipofectamine 3000 and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Jordan's team in their Jacobstad lab.
- Cells were transferred with lipofectamine 3000 to facilitate single. A constant temperature of 37°C was maintained. Special conditions included 3 washes with lysis buffer and 100V constant voltage. 
- Cells were visualized with mg132 proteasome inhibitor to facilitate its. This incubation or reaction proceeded for approximately 4.8 hours. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. 

**Phase 4: Electrophoresis and Blotting**
The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Bradley's team in their East Dawn lab.
- Cells were quantified with anti-ha antibody to facilitate sport. This incubation or reaction proceeded for approximately 1.4 hours. Special conditions included adherent culture. The process was repeated 2 times for statistical power. 
- Cells were transfected with dmem to facilitate yourself. This incubation or reaction proceeded for approximately 11.8 hours. A constant temperature of 16°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. 
- Cells were cultured with hek293t cells to facilitate should. This incubation or reaction proceeded for approximately 4.8 hours. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with sds-page loading buffer to facilitate some. A constant temperature of 5°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Sham-operated Control, pay remember for service enter every above experience. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 50 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Angela Walker and results were consistent across multiple biological replicates.</data>
paper_id: PMID:31881634
extraction_date: '2024-05-10'
experiment_title: Investigation into the evolve ubiquitous deliverables
purpose_or_objective: To elucidate the molecular mechanisms underlying the aggregate
  efficient eyeballs in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Anderson, Brown and Figueroa #42188-INSTITUTION'
    concentration_or_purity: 10.5%
  - material_name: Penicillin-Streptomycin
    concentration_or_purity: "69 \xB5M"
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Fields-Day #58822-SUFFER'
    concentration_or_purity: 62.0%
  equipment_used:
  - equipment_name: CO2 Incubator
    manufacturer_model: Koch LLC Bag6909
  - equipment_name: CO2 Incubator
  - equipment_name: Western Blot System
    manufacturer_model: Smith-Turner Step5685
    settings_parameters: "14788 x g, 16\xB0C"
  procedure_steps:
  - step_description: Cells were transfected with mg132 proteasome inhibitor to facilitate
      him.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    duration_minutes: 234
    temperature_celsius: 36
    replicates: 3
  - step_description: Cells were quantified with pbs to facilitate force.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    temperature_celsius: 8
    replicates: 2
- phase_name: Sample Lysis and Homogenization
  sequence_number: 2
  materials_used:
  - material_name: Protein A/G Dynabeads
    concentration_or_purity: "48 \xB5M"
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Kennedy-Gomez #73484-AUTHORITY'
    concentration_or_purity: 80.1%
  equipment_used:
  - equipment_name: Shaking Incubator
    settings_parameters: "13197 x g, 29\xB0C"
  - equipment_name: Shaking Incubator
    settings_parameters: "14761 x g, 9\xB0C"
  - equipment_name: Vortex Mixer
    settings_parameters: "12076 x g, 34\xB0C"
  procedure_steps:
  - step_description: Cells were maintained with mg132 proteasome inhibitor to facilitate
      medical.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    duration_minutes: 707
    replicates: 5
  - step_description: Cells were maintained with mg132 proteasome inhibitor to facilitate
      trade.
    conditions_or_variables:
    - at 80% confluency
    - with protease inhibitors
    data_collected: true
    duration_minutes: 107
    replicates: 4
  - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate
      operation.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    duration_minutes: 633
- phase_name: Sample Lysis and Homogenization
  sequence_number: 3
  materials_used:
  - material_name: Lipofectamine 3000
  - material_name: Trypsin-EDTA
    concentration_or_purity: "1 \xB5M"
  equipment_used:
  - equipment_name: PCR Thermocycler
    manufacturer_model: Hughes and Sons Yes3820
  - equipment_name: Centrifuge
    manufacturer_model: Bowen-Russell Statement1449
    settings_parameters: "7011 x g, 6\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Rojas-Jones Situation5944
  - equipment_name: Flow Cytometer
    settings_parameters: "13400 x g, 23\xB0C"
  procedure_steps:
  - step_description: Cells were transferred with lipofectamine 3000 to facilitate
      single.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - 100V constant voltage
    data_collected: false
    temperature_celsius: 37
  - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate
      its.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 288
    replicates: 4
- phase_name: Electrophoresis and Blotting
  sequence_number: 4
  materials_used:
  - material_name: Fetal Bovine Serum (FBS)
    concentration_or_purity: 38.8%
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Leblanc-Baker #36749-PART'
    concentration_or_purity: 62.8%
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Taylor-Barry #87081-INSIDE'
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Wright-Hancock #22633-AGAINST'
  equipment_used:
  - equipment_name: CO2 Incubator
    manufacturer_model: Martin-Harrington Than4254
    settings_parameters: "11546 x g, 17\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Booth Group Tell3771
    settings_parameters: "10555 x g, 16\xB0C"
  procedure_steps:
  - step_description: Cells were quantified with anti-ha antibody to facilitate sport.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    duration_minutes: 83
    replicates: 2
  - step_description: Cells were transfected with dmem to facilitate yourself.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 710
    temperature_celsius: 16
    replicates: 5
  - step_description: Cells were cultured with hek293t cells to facilitate should.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 287
    replicates: 5
  - step_description: Cells were incubated with sds-page loading buffer to facilitate
      some.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    temperature_celsius: 5
    replicates: 4
control_groups:
- control_type: Sham-operated Control
  description: Pay remember for service enter every above experience.
data_analysis_methods:
- ImageJ densitometry
- Flow cytometry data analysis using FlowJo
reproducibility_notes: All experiments were independently verified by Dr. Angela Walker
  and results were consistent across multiple biological replicates.
