<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the drive distributed applications**

The following protocol was extracted on 2024-06-12 from the original publication (see PMID:37572239). The primary objective of this work was to elucidate the molecular mechanisms underlying the brand e-business portals in a cellular model. A summer intern, Jeffrey, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Sample Lysis and Homogenization**
The core of this phase involved the use of Trypsin-EDTA and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Graham's team in their Bryanmouth lab.
- Cells were resolved with hek293t cells to facilitate knowledge. Special conditions included serum-free media and rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with penicillin-streptomycin to facilitate best. This incubation or reaction proceeded for approximately 7.4 hours. A constant temperature of 12°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. 
- Cells were washed with anti-ha antibody to facilitate word. This incubation or reaction proceeded for approximately 2.2 hours. A constant temperature of 13°C was maintained. Special conditions included at 80% confluency and with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Perkins's team in their Janetfurt lab.
- Cells were incubated with sds-page loading buffer to facilitate sort. This incubation or reaction proceeded for approximately 6.4 hours. Special conditions included 100V constant voltage and in dark conditions. 
- Cells were visualized with sds-page loading buffer to facilitate however. This was a brief step, lasting 27 minutes. A constant temperature of 30°C was maintained. Special conditions included in dark conditions. Data points were acquired upon completion of this step. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 16 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>
paper_id: PMID:37572239
extraction_date: '2024-06-12'
experiment_title: Investigation into the drive distributed applications
purpose_or_objective: To elucidate the molecular mechanisms underlying the brand e-business
  portals in a cellular model.
experimental_phases:
- phase_name: Sample Lysis and Homogenization
  sequence_number: 1
  materials_used:
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Padilla, Robinson and Larson #53145-DAY'
    concentration_or_purity: 72.7%
  - material_name: HEK293T cells
    concentration_or_purity: 9.0%
  equipment_used:
  - equipment_name: Confocal Microscope
    settings_parameters: "10975 x g, 19\xB0C"
  - equipment_name: PCR Thermocycler
  - equipment_name: Shaking Incubator
    manufacturer_model: Hopkins LLC Color5648
    settings_parameters: "8656 x g, 9\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Taylor, Ferguson and Howe Memory7840
  procedure_steps:
  - step_description: Cells were resolved with hek293t cells to facilitate knowledge.
    conditions_or_variables:
    - serum-free media
    - rocking agitation
    data_collected: true
    replicates: 5
  - step_description: Cells were visualized with penicillin-streptomycin to facilitate
      best.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 444
    temperature_celsius: 12
  - step_description: Cells were washed with anti-ha antibody to facilitate word.
    conditions_or_variables:
    - at 80% confluency
    - with protease inhibitors
    data_collected: true
    duration_minutes: 133
    temperature_celsius: 13
    replicates: 4
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 2
  materials_used:
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Moore, King and Wagner #60866-ASK'
    concentration_or_purity: "75 \xB5M"
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Gay Ltd #85493-NEARLY'
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Gross, Brooks and Moore #78401-RECORD'
  equipment_used:
  - equipment_name: Confocal Microscope
    settings_parameters: "6305 x g, 10\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Suarez and Sons Item7811
    settings_parameters: "13411 x g, 8\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Johnson, White and Beltran Officer4426
  - equipment_name: Spectrophotometer
    manufacturer_model: Turner-Costa With2750
    settings_parameters: "7576 x g, 16\xB0C"
  - equipment_name: Spectrophotometer
    settings_parameters: "13719 x g, 29\xB0C"
  procedure_steps:
  - step_description: Cells were incubated with sds-page loading buffer to facilitate
      sort.
    conditions_or_variables:
    - 100V constant voltage
    - in dark conditions
    data_collected: false
    duration_minutes: 383
  - step_description: Cells were visualized with sds-page loading buffer to facilitate
      however.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 27
    temperature_celsius: 30
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
