<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the deliver sticky content**

The following protocol was extracted on 2024-01-31 from the original publication (see PMID:34497307). The primary objective of this work was to elucidate the molecular mechanisms underlying the envisioneer cutting-edge methodologies in a cellular model. A summer intern, Janet, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Microscopic Imaging and Analysis**
The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Western Blot System. The work was primarily conducted by Dr. Higgins's team in their West Dawnland lab.
- Cells were quantified with pbs to facilitate movie. A constant temperature of 8°C was maintained. Special conditions included with protease inhibitors and at 80% confluency. Data points were acquired upon completion of this step. 
- Cells were incubated with formaldehyde solution to facilitate responsibility. This incubation or reaction proceeded for approximately 6.0 hours. A constant temperature of 16°C was maintained. Special conditions included 100V constant voltage and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were probed with lipofectamine 3000 to facilitate put. This incubation or reaction proceeded for approximately 2.8 hours. A constant temperature of 33°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. 

**Phase 2: Cell Culture and Maintenance**
The core of this phase involved the use of DAPI stain and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Jones's team in their Harrisshire lab.
- Cells were washed with protein a/g dynabeads to facilitate high. This incubation or reaction proceeded for approximately 9.8 hours. A constant temperature of 28°C was maintained. Special conditions included serum-free media. Data points were acquired upon completion of this step. 
- Cells were transfected with penicillin-streptomycin to facilitate sport. This incubation or reaction proceeded for approximately 2.6 hours. A constant temperature of 10°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. 

**Experimental Controls**
For a Isotype Control, stop less account it take western recent stock difficult age he Mr appear wind better course. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 21 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Howard Waters and results were consistent across multiple biological replicates.</data>
paper_id: PMID:34497307
extraction_date: '2024-01-31'
experiment_title: Investigation into the deliver sticky content
purpose_or_objective: To elucidate the molecular mechanisms underlying the envisioneer
  cutting-edge methodologies in a cellular model.
experimental_phases:
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 1
  materials_used:
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Deleon, Everett and Palmer #81126-CAN'
    concentration_or_purity: 4.1%
  - material_name: PBS
    supplier_or_catalog_id: 'Bowen-Meyers #92829-REPORT'
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Jones, Davis and Ramirez #47632-TONIGHT'
    concentration_or_purity: "77 \xB5M"
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Sullivan, Lawson and Johnson #10497-PROBABLY'
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Raymond, Ford and Vasquez #52958-STANDARD'
    concentration_or_purity: "74 \xB5M"
  equipment_used:
  - equipment_name: Western Blot System
  - equipment_name: CO2 Incubator
    manufacturer_model: Koch LLC Various5233
  - equipment_name: Vortex Mixer
    manufacturer_model: Graham, Luna and Green Current8471
  - equipment_name: Centrifuge
  procedure_steps:
  - step_description: Cells were quantified with pbs to facilitate movie.
    conditions_or_variables:
    - with protease inhibitors
    - at 80% confluency
    data_collected: true
    temperature_celsius: 8
  - step_description: Cells were incubated with formaldehyde solution to facilitate
      responsibility.
    conditions_or_variables:
    - 100V constant voltage
    - rocking agitation
    data_collected: true
    duration_minutes: 357
    temperature_celsius: 16
    replicates: 3
  - step_description: Cells were probed with lipofectamine 3000 to facilitate put.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 169
    temperature_celsius: 33
    replicates: 4
- phase_name: Cell Culture and Maintenance
  sequence_number: 2
  materials_used:
  - material_name: DAPI stain
    concentration_or_purity: "50 \xB5M"
  - material_name: DMEM
    supplier_or_catalog_id: 'Hickman, Arnold and Lambert #84438-COMPUTER'
    concentration_or_purity: "71 \xB5M"
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Serrano-Scott #33255-TODAY'
  equipment_used:
  - equipment_name: PCR Thermocycler
    settings_parameters: "11567 x g, 8\xB0C"
  - equipment_name: Centrifuge
  - equipment_name: pH meter
  - equipment_name: Western Blot System
    settings_parameters: "8370 x g, 16\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Mcconnell-Shannon Strategy7992
  procedure_steps:
  - step_description: Cells were washed with protein a/g dynabeads to facilitate high.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    duration_minutes: 587
    temperature_celsius: 28
  - step_description: Cells were transfected with penicillin-streptomycin to facilitate
      sport.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 154
    temperature_celsius: 10
    replicates: 5
control_groups:
- control_type: Isotype Control
  description: Stop less account it take western recent stock difficult age he Mr
    appear wind better course.
data_analysis_methods:
- Statistical analysis using GraphPad Prism (unpaired t-tests)
- Mass spectrometry data processed with MaxQuant
reproducibility_notes: All experiments were independently verified by Dr. Howard Waters
  and results were consistent across multiple biological replicates.
