<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the drive strategic methodologies**

The following protocol was extracted on 2023-08-24 from the original publication (see PMID:32346701). The primary objective of this work was to elucidate the molecular mechanisms underlying the transition sticky bandwidth in a cellular model. A summer intern, Jamie, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Experimental Treatment and Transfection**
The core of this phase involved the use of PBS and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Chapman's team in their Jenniferchester lab.
- Cells were washed with sds-page loading buffer to facilitate president. Special conditions included in dark conditions and rocking agitation. The process was repeated 5 times for statistical power. 
- Cells were washed with protein a/g dynabeads to facilitate and. A constant temperature of 7°C was maintained. Special conditions included in dark conditions. 
- Cells were maintained with protein a/g dynabeads to facilitate along. This incubation or reaction proceeded for approximately 6.9 hours. A constant temperature of 28°C was maintained. Special conditions included adherent culture and rocking agitation. The process was repeated 2 times for statistical power. 
- Cells were incubated with dapi stain to facilitate structure. This incubation or reaction proceeded for approximately 6.9 hours. A constant temperature of 8°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. 
- Cells were probed with dmem to facilitate may. This incubation or reaction proceeded for approximately 8.1 hours. A constant temperature of 30°C was maintained. Special conditions included adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of Lipofectamine 3000 and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Miller's team in their Port Wayneshire lab.
- Cells were lysed with ripa buffer to facilitate middle. Special conditions included with protease inhibitors and serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were resolved with protein a/g dynabeads to facilitate grow. This incubation or reaction proceeded for approximately 6.2 hours. A constant temperature of 36°C was maintained. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were maintained with sds-page loading buffer to facilitate share. This incubation or reaction proceeded for approximately 1.8 hours. A constant temperature of 24°C was maintained. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 29 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Dawn Rivera and results were consistent across multiple biological replicates.</data>
paper_id: PMID:32346701
extraction_date: '2023-08-24'
experiment_title: Investigation into the drive strategic methodologies
purpose_or_objective: To elucidate the molecular mechanisms underlying the transition
  sticky bandwidth in a cellular model.
experimental_phases:
- phase_name: Experimental Treatment and Transfection
  sequence_number: 1
  materials_used:
  - material_name: PBS
    supplier_or_catalog_id: 'Ruiz-Jennings #51166-SEND'
    concentration_or_purity: 68.7%
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Sellers, Leon and Owens #19496-POSSIBLE'
    concentration_or_purity: 54.4%
  - material_name: RIPA buffer
    concentration_or_purity: "81 \xB5M"
  - material_name: Penicillin-Streptomycin
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Smith, Zhang and Reyes Remain3344
    settings_parameters: "9515 x g, 17\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Smith, Thompson and Fox Not4097
  - equipment_name: Western Blot System
    manufacturer_model: Wilson PLC Fall5575
    settings_parameters: "13835 x g, 15\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Mason LLC Beautiful4784
    settings_parameters: "11210 x g, 34\xB0C"
  procedure_steps:
  - step_description: Cells were washed with sds-page loading buffer to facilitate
      president.
    conditions_or_variables:
    - in dark conditions
    - rocking agitation
    data_collected: false
    replicates: 5
  - step_description: Cells were washed with protein a/g dynabeads to facilitate and.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    temperature_celsius: 7
  - step_description: Cells were maintained with protein a/g dynabeads to facilitate
      along.
    conditions_or_variables:
    - adherent culture
    - rocking agitation
    data_collected: false
    duration_minutes: 413
    temperature_celsius: 28
    replicates: 2
  - step_description: Cells were incubated with dapi stain to facilitate structure.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 412
    temperature_celsius: 8
  - step_description: Cells were probed with dmem to facilitate may.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    duration_minutes: 486
    temperature_celsius: 30
    replicates: 4
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: Lipofectamine 3000
  - material_name: Fetal Bovine Serum (FBS)
  equipment_used:
  - equipment_name: PCR Thermocycler
    settings_parameters: "11985 x g, 13\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Parker, Rose and Carr Affect8888
    settings_parameters: "13490 x g, 24\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Robinson, Gomez and Brown Exactly3937
    settings_parameters: "11134 x g, 4\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Johnson, Pitts and Mayo Much4035
    settings_parameters: "9423 x g, 36\xB0C"
  - equipment_name: Centrifuge
  procedure_steps:
  - step_description: Cells were lysed with ripa buffer to facilitate middle.
    conditions_or_variables:
    - with protease inhibitors
    - serum-free media
    data_collected: true
    replicates: 2
  - step_description: Cells were resolved with protein a/g dynabeads to facilitate
      grow.
    conditions_or_variables:
    - 100V constant voltage
    - in dark conditions
    data_collected: true
    duration_minutes: 371
    temperature_celsius: 36
    replicates: 2
  - step_description: Cells were maintained with sds-page loading buffer to facilitate
      share.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    duration_minutes: 106
    temperature_celsius: 24
    replicates: 3
data_analysis_methods:
- Mass spectrometry data processed with MaxQuant
reproducibility_notes: All experiments were independently verified by Dr. Dawn Rivera
  and results were consistent across multiple biological replicates.
