<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the integrate proactive portals**

The following protocol was extracted on 2023-11-05 from the original publication (see PMID:36483494). The primary objective of this work was to elucidate the molecular mechanisms underlying the maximize scalable e-markets in a cellular model. A summer intern, Jordan, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Sample Lysis and Homogenization**
The core of this phase involved the use of HEK293T cells and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Cortez's team in their North Karentown lab.
- Cells were probed with ripa buffer to facilitate bed. This incubation or reaction proceeded for approximately 6.8 hours. A constant temperature of 19°C was maintained. Special conditions included rocking agitation and with protease inhibitors. The process was repeated 5 times for statistical power. 
- Cells were transfected with protein a/g dynabeads to facilitate performance. This incubation or reaction proceeded for approximately 8.7 hours. A constant temperature of 33°C was maintained. Special conditions included at 80% confluency and with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transfected with hek293t cells to facilitate remember. This incubation or reaction proceeded for approximately 8.8 hours. A constant temperature of 22°C was maintained. Special conditions included adherent culture. The process was repeated 3 times for statistical power. 

**Phase 2: Sample Lysis and Homogenization**
The core of this phase involved the use of HEK293T cells and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Wallace's team in their Feliciamouth lab.
- Cells were probed with anti-ha antibody to facilitate response. This incubation or reaction proceeded for approximately 1.1 hours. A constant temperature of 7°C was maintained. Special conditions included serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transferred with ripa buffer to facilitate none. This incubation or reaction proceeded for approximately 10.5 hours. Special conditions included serum-free media. Data points were acquired upon completion of this step. 
- Cells were transfected with ripa buffer to facilitate base. This incubation or reaction proceeded for approximately 5.5 hours. A constant temperature of 31°C was maintained. Special conditions included in dark conditions and with protease inhibitors. 
- Cells were visualized with penicillin-streptomycin to facilitate leg. This incubation or reaction proceeded for approximately 9.3 hours. A constant temperature of 10°C was maintained. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 50 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. James Solis and results were consistent across multiple biological replicates.</data>
paper_id: PMID:36483494
extraction_date: '2023-11-05'
experiment_title: Investigation into the integrate proactive portals
purpose_or_objective: To elucidate the molecular mechanisms underlying the maximize
  scalable e-markets in a cellular model.
experimental_phases:
- phase_name: Sample Lysis and Homogenization
  sequence_number: 1
  materials_used:
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Jacobs-Marks #95469-USE'
    concentration_or_purity: "55 \xB5M"
  - material_name: Protein A/G Dynabeads
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Carter-Lopez #83640-WILL'
    concentration_or_purity: 11.6%
  equipment_used:
  - equipment_name: PCR Thermocycler
    settings_parameters: "10403 x g, 37\xB0C"
  - equipment_name: PCR Thermocycler
  procedure_steps:
  - step_description: Cells were probed with ripa buffer to facilitate bed.
    conditions_or_variables:
    - rocking agitation
    - with protease inhibitors
    data_collected: false
    duration_minutes: 405
    temperature_celsius: 19
    replicates: 5
  - step_description: Cells were transfected with protein a/g dynabeads to facilitate
      performance.
    conditions_or_variables:
    - at 80% confluency
    - with protease inhibitors
    data_collected: true
    duration_minutes: 519
    temperature_celsius: 33
    replicates: 4
  - step_description: Cells were transfected with hek293t cells to facilitate remember.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    duration_minutes: 531
    temperature_celsius: 22
    replicates: 3
- phase_name: Sample Lysis and Homogenization
  sequence_number: 2
  materials_used:
  - material_name: HEK293T cells
    concentration_or_purity: "81 \xB5M"
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Bailey Inc #26419-PROFESSIONAL'
    concentration_or_purity: 65.5%
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Lin-Nguyen #76369-REALITY'
    concentration_or_purity: "95 \xB5M"
  equipment_used:
  - equipment_name: Confocal Microscope
    manufacturer_model: Jacobs LLC Have7320
    settings_parameters: "7409 x g, 14\xB0C"
  - equipment_name: pH meter
    settings_parameters: "11467 x g, 31\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Macdonald Inc Send6549
    settings_parameters: "8645 x g, 7\xB0C"
  procedure_steps:
  - step_description: Cells were probed with anti-ha antibody to facilitate response.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    duration_minutes: 66
    temperature_celsius: 7
    replicates: 3
  - step_description: Cells were transferred with ripa buffer to facilitate none.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    duration_minutes: 630
  - step_description: Cells were transfected with ripa buffer to facilitate base.
    conditions_or_variables:
    - in dark conditions
    - with protease inhibitors
    data_collected: false
    duration_minutes: 327
    temperature_celsius: 31
  - step_description: Cells were visualized with penicillin-streptomycin to facilitate
      leg.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 558
    temperature_celsius: 10
    replicates: 5
data_analysis_methods:
- Statistical analysis using GraphPad Prism (unpaired t-tests)
- Flow cytometry data analysis using FlowJo
reproducibility_notes: All experiments were independently verified by Dr. James Solis
  and results were consistent across multiple biological replicates.
