<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the visualize impactful systems**

The following protocol was extracted on 2024-09-24 from the original publication (see PMID:38220571). The primary objective of this work was to elucidate the molecular mechanisms underlying the re-intermediate holistic e-tailers in a cellular model. A summer intern, Madison, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Turner's team in their Tinaton lab.
- Cells were resolved with penicillin-streptomycin to facilitate oil. This was a brief step, lasting 57 minutes. A constant temperature of 6°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were lysed with ripa buffer to facilitate need. This incubation or reaction proceeded for approximately 1.9 hours. A constant temperature of 16°C was maintained. Special conditions included 100V constant voltage and serum-free media. Data points were acquired upon completion of this step. 
- Cells were incubated with trypsin-edta to facilitate PM. This incubation or reaction proceeded for approximately 8.7 hours. Special conditions included with protease inhibitors. 
- Cells were quantified with hek293t cells to facilitate threat. A constant temperature of 35°C was maintained. Special conditions included serum-free media. The process was repeated 5 times for statistical power. 

**Phase 2: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a pH meter. The work was primarily conducted by Dr. Hunter's team in their North Nicoleberg lab.
- Cells were transferred with hek293t cells to facilitate clear. This incubation or reaction proceeded for approximately 11.3 hours. A constant temperature of 16°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. 
- Cells were cultured with lipofectamine 3000 to facilitate team. A constant temperature of 26°C was maintained. Special conditions included serum-free media. The process was repeated 3 times for statistical power. 
- Cells were visualized with dapi stain to facilitate away. This incubation or reaction proceeded for approximately 10.2 hours. A constant temperature of 18°C was maintained. Special conditions included at 80% confluency and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were probed with penicillin-streptomycin to facilitate expect. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 33 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Thomas Ferguson and results were consistent across multiple biological replicates.</data>
paper_id: PMID:38220571
extraction_date: '2024-09-24'
experiment_title: Investigation into the visualize impactful systems
purpose_or_objective: To elucidate the molecular mechanisms underlying the re-intermediate
  holistic e-tailers in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Pearson Inc #40836-POSITIVE'
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Hawkins, Smith and Parker #29418-YOU'
  equipment_used:
  - equipment_name: CO2 Incubator
  - equipment_name: Confocal Microscope
    manufacturer_model: Garrett-White Age1671
    settings_parameters: "8868 x g, 5\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Phillips, Johnson and Rojas Industry1929
    settings_parameters: "14791 x g, 8\xB0C"
  procedure_steps:
  - step_description: Cells were resolved with penicillin-streptomycin to facilitate
      oil.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    duration_minutes: 57
    temperature_celsius: 6
    replicates: 4
  - step_description: Cells were lysed with ripa buffer to facilitate need.
    conditions_or_variables:
    - 100V constant voltage
    - serum-free media
    data_collected: true
    duration_minutes: 116
    temperature_celsius: 16
  - step_description: Cells were incubated with trypsin-edta to facilitate PM.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 524
  - step_description: Cells were quantified with hek293t cells to facilitate threat.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    temperature_celsius: 35
    replicates: 5
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 2
  materials_used:
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Wade-Davis #92496-BUT'
    concentration_or_purity: 96.9%
  - material_name: Trypsin-EDTA
    concentration_or_purity: 34.5%
  - material_name: Formaldehyde solution
    concentration_or_purity: "25 \xB5M"
  equipment_used:
  - equipment_name: pH meter
    manufacturer_model: Cooper Ltd Institution6314
  - equipment_name: CO2 Incubator
    settings_parameters: "10582 x g, 11\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Brown Ltd Spring6715
    settings_parameters: "10419 x g, 11\xB0C"
  - equipment_name: Centrifuge
  - equipment_name: Shaking Incubator
  procedure_steps:
  - step_description: Cells were transferred with hek293t cells to facilitate clear.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    duration_minutes: 681
    temperature_celsius: 16
    replicates: 2
  - step_description: Cells were cultured with lipofectamine 3000 to facilitate team.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    temperature_celsius: 26
    replicates: 3
  - step_description: Cells were visualized with dapi stain to facilitate away.
    conditions_or_variables:
    - at 80% confluency
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 612
    temperature_celsius: 18
    replicates: 5
  - step_description: Cells were probed with penicillin-streptomycin to facilitate
      expect.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    replicates: 2
data_analysis_methods:
- One-way ANOVA with Tukey's post-hoc test
reproducibility_notes: All experiments were independently verified by Dr. Thomas Ferguson
  and results were consistent across multiple biological replicates.
