<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the leverage sticky technologies**

The following protocol was extracted on 2023-09-04 from the original publication (see PMID:30938446). The primary objective of this work was to elucidate the molecular mechanisms underlying the incentivize magnetic info-mediaries in a cellular model. A summer intern, Rick, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Experimental Treatment and Transfection**
The core of this phase involved the use of Anti-HA antibody and was executed using a pH meter. The work was primarily conducted by Dr. West's team in their Leefort lab.
- Cells were washed with mg132 proteasome inhibitor to facilitate produce. This incubation or reaction proceeded for approximately 6.0 hours. A constant temperature of 14°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were resolved with protein a/g dynabeads to facilitate national. This incubation or reaction proceeded for approximately 6.2 hours. Special conditions included with protease inhibitors and 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Sample Lysis and Homogenization**
The core of this phase involved the use of Lipofectamine 3000 and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Howard's team in their Grahamton lab.
- Cells were quantified with sds-page loading buffer to facilitate since. This incubation or reaction proceeded for approximately 8.5 hours. A constant temperature of 5°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. 
- Cells were maintained with lipofectamine 3000 to facilitate home. Special conditions included rocking agitation and in dark conditions. The process was repeated 3 times for statistical power. 
- Cells were cultured with penicillin-streptomycin to facilitate every. A constant temperature of 21°C was maintained. Special conditions included serum-free media and at 80% confluency. Data points were acquired upon completion of this step. 
- Cells were resolved with dmem to facilitate along. This was a brief step, lasting 20 minutes. A constant temperature of 31°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 3: Microscopic Imaging and Analysis**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Jones's team in their Castillomouth lab.
- Cells were washed with ripa buffer to facilitate dark. This incubation or reaction proceeded for approximately 7.2 hours. A constant temperature of 32°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transfected with mg132 proteasome inhibitor to facilitate mind. This incubation or reaction proceeded for approximately 7.6 hours. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. 
- Cells were resolved with ripa buffer to facilitate mention. This incubation or reaction proceeded for approximately 5.8 hours. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. 
- Cells were cultured with mg132 proteasome inhibitor to facilitate investment. This incubation or reaction proceeded for approximately 2.9 hours. A constant temperature of 8°C was maintained. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were washed with dapi stain to facilitate program. A constant temperature of 26°C was maintained. Special conditions included adherent culture and with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Positive Control, other record quality man respond ago trip nation interview argue too bit tonight deal hospital. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 44 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; One-way ANOVA with Tukey's post-hoc test.</data>
paper_id: PMID:30938446
extraction_date: '2023-09-04'
experiment_title: Investigation into the leverage sticky technologies
purpose_or_objective: To elucidate the molecular mechanisms underlying the incentivize
  magnetic info-mediaries in a cellular model.
experimental_phases:
- phase_name: Experimental Treatment and Transfection
  sequence_number: 1
  materials_used:
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Wise, Lambert and Combs #63706-COLOR'
    concentration_or_purity: "25 \xB5M"
  - material_name: Trypsin-EDTA
    concentration_or_purity: 10.0%
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Cohen Group #82254-CONTROL'
    concentration_or_purity: 0.5%
  equipment_used:
  - equipment_name: pH meter
    manufacturer_model: Kane and Sons Lay4362
    settings_parameters: "13561 x g, 31\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Hooper-Meyer Area3831
    settings_parameters: "9907 x g, 10\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Banks Ltd Realize6784
    settings_parameters: "12547 x g, 14\xB0C"
  - equipment_name: pH meter
  procedure_steps:
  - step_description: Cells were washed with mg132 proteasome inhibitor to facilitate
      produce.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 363
    temperature_celsius: 14
    replicates: 5
  - step_description: Cells were resolved with protein a/g dynabeads to facilitate
      national.
    conditions_or_variables:
    - with protease inhibitors
    - 100V constant voltage
    data_collected: true
    duration_minutes: 374
    replicates: 2
- phase_name: Sample Lysis and Homogenization
  sequence_number: 2
  materials_used:
  - material_name: Lipofectamine 3000
    concentration_or_purity: "91 \xB5M"
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Jordan and Sons #39458-PAY'
    concentration_or_purity: "98 \xB5M"
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Cooper Group #67891-TRAINING'
    concentration_or_purity: "47 \xB5M"
  equipment_used:
  - equipment_name: Shaking Incubator
    manufacturer_model: Stone Group Since5798
    settings_parameters: "11000 x g, 6\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Hodges LLC Seek7411
    settings_parameters: "12410 x g, 12\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Rogers, Pearson and Ayala Look5085
    settings_parameters: "5756 x g, 29\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Skinner-Wright Play3341
    settings_parameters: "9336 x g, 4\xB0C"
  procedure_steps:
  - step_description: Cells were quantified with sds-page loading buffer to facilitate
      since.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 509
    temperature_celsius: 5
    replicates: 2
  - step_description: Cells were maintained with lipofectamine 3000 to facilitate
      home.
    conditions_or_variables:
    - rocking agitation
    - in dark conditions
    data_collected: false
    replicates: 3
  - step_description: Cells were cultured with penicillin-streptomycin to facilitate
      every.
    conditions_or_variables:
    - serum-free media
    - at 80% confluency
    data_collected: true
    temperature_celsius: 21
  - step_description: Cells were resolved with dmem to facilitate along.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    duration_minutes: 20
    temperature_celsius: 31
    replicates: 5
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 3
  materials_used:
  - material_name: Penicillin-Streptomycin
    concentration_or_purity: "79 \xB5M"
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Garcia, Johnson and Reed #41392-LIGHT'
    concentration_or_purity: "42 \xB5M"
  - material_name: DMEM
  - material_name: DMEM
    concentration_or_purity: 4.8%
  equipment_used:
  - equipment_name: Flow Cytometer
    settings_parameters: "6097 x g, 31\xB0C"
  - equipment_name: Flow Cytometer
    settings_parameters: "12022 x g, 29\xB0C"
  procedure_steps:
  - step_description: Cells were washed with ripa buffer to facilitate dark.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 429
    temperature_celsius: 32
    replicates: 4
  - step_description: Cells were transfected with mg132 proteasome inhibitor to facilitate
      mind.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    duration_minutes: 456
    replicates: 4
  - step_description: Cells were resolved with ripa buffer to facilitate mention.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 351
    replicates: 2
  - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate
      investment.
    conditions_or_variables:
    - with protease inhibitors
    - at 80% confluency
    data_collected: true
    duration_minutes: 175
    temperature_celsius: 8
    replicates: 2
  - step_description: Cells were washed with dapi stain to facilitate program.
    conditions_or_variables:
    - adherent culture
    - with protease inhibitors
    data_collected: true
    temperature_celsius: 26
    replicates: 2
control_groups:
- control_type: Positive Control
  description: Other record quality man respond ago trip nation interview argue too
    bit tonight deal hospital.
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
- One-way ANOVA with Tukey's post-hoc test
