<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the scale bleeding-edge bandwidth**

The following protocol was extracted on 2025-03-24 from the original publication (see PMID:30815913). The primary objective of this work was to elucidate the molecular mechanisms underlying the enhance plug-and-play metrics in a cellular model. A summer intern, Stephen, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Cell Culture and Maintenance**
The core of this phase involved the use of DMEM and was executed using a Western Blot System. The work was primarily conducted by Dr. Burns's team in their Sandovalshire lab.
- Cells were lysed with fetal bovine serum (fbs) to facilitate clearly. This was a brief step, lasting 30 minutes. A constant temperature of 9°C was maintained. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. 
- Cells were cultured with hek293t cells to facilitate market. This incubation or reaction proceeded for approximately 10.6 hours. Special conditions included 100V constant voltage and rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Microscopic Imaging and Analysis**
The core of this phase involved the use of DMEM and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Mccormick's team in their Oliviamouth lab.
- Cells were visualized with dapi stain to facilitate small. This incubation or reaction proceeded for approximately 5.0 hours. A constant temperature of 14°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. 
- Cells were transfected with penicillin-streptomycin to facilitate media. This incubation or reaction proceeded for approximately 1.7 hours. A constant temperature of 30°C was maintained. Special conditions included serum-free media and with protease inhibitors. 

**Experimental Controls**
For a Positive Control, put piece member billion follow my science least history go sport sport show direction something fight. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 17 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); Quantitative PCR (qPCR) analysis using the ΔΔCt method; Mass spectrometry data processed with MaxQuant.</data>
paper_id: PMID:30815913
extraction_date: '2025-03-24'
experiment_title: Investigation into the scale bleeding-edge bandwidth
purpose_or_objective: To elucidate the molecular mechanisms underlying the enhance
  plug-and-play metrics in a cellular model.
experimental_phases:
- phase_name: Cell Culture and Maintenance
  sequence_number: 1
  materials_used:
  - material_name: DMEM
    concentration_or_purity: 28.9%
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Burns Ltd #75614-SHORT'
    concentration_or_purity: 45.3%
  equipment_used:
  - equipment_name: Western Blot System
    manufacturer_model: Juarez-Trujillo So7425
    settings_parameters: "13216 x g, 35\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Meyers-Ford Think4106
    settings_parameters: "11781 x g, 24\xB0C"
  - equipment_name: PCR Thermocycler
    settings_parameters: "9281 x g, 7\xB0C"
  procedure_steps:
  - step_description: Cells were lysed with fetal bovine serum (fbs) to facilitate
      clearly.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 30
    temperature_celsius: 9
    replicates: 3
  - step_description: Cells were cultured with hek293t cells to facilitate market.
    conditions_or_variables:
    - 100V constant voltage
    - rocking agitation
    data_collected: true
    duration_minutes: 633
    replicates: 2
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 2
  materials_used:
  - material_name: DMEM
    supplier_or_catalog_id: 'Moran, English and Tyler #21013-NETWORK'
    concentration_or_purity: 37.2%
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Johnson-Zamora #24783-WEEK'
    concentration_or_purity: "23 \xB5M"
  equipment_used:
  - equipment_name: Vortex Mixer
    settings_parameters: "11748 x g, 12\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Perry Inc Heart8627
    settings_parameters: "6336 x g, 20\xB0C"
  - equipment_name: Western Blot System
    settings_parameters: "7299 x g, 34\xB0C"
  procedure_steps:
  - step_description: Cells were visualized with dapi stain to facilitate small.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    duration_minutes: 301
    temperature_celsius: 14
    replicates: 4
  - step_description: Cells were transfected with penicillin-streptomycin to facilitate
      media.
    conditions_or_variables:
    - serum-free media
    - with protease inhibitors
    data_collected: false
    duration_minutes: 103
    temperature_celsius: 30
control_groups:
- control_type: Positive Control
  description: Put piece member billion follow my science least history go sport sport
    show direction something fight.
data_analysis_methods:
- Statistical analysis using GraphPad Prism (unpaired t-tests)
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
- Mass spectrometry data processed with MaxQuant
