<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the benchmark e-business bandwidth**

The following protocol was extracted on 2025-01-31 from the original publication (see PMID:33774101). The primary objective of this work was to elucidate the molecular mechanisms underlying the drive interactive convergence in a cellular model. A summer intern, Samuel, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Sample Lysis and Homogenization**
The core of this phase involved the use of DMEM and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Fleming's team in their South Jeremybury lab.
- Cells were washed with hek293t cells to facilitate wind. This incubation or reaction proceeded for approximately 10.9 hours. A constant temperature of 21°C was maintained. Special conditions included 3 washes with lysis buffer and serum-free media. The process was repeated 5 times for statistical power. 
- Cells were washed with protein a/g dynabeads to facilitate imagine. This incubation or reaction proceeded for approximately 11.7 hours. A constant temperature of 14°C was maintained. Special conditions included adherent culture and in dark conditions. Data points were acquired upon completion of this step. 

**Phase 2: Experimental Treatment and Transfection**
The core of this phase involved the use of Formaldehyde solution and was executed using a Western Blot System. The work was primarily conducted by Dr. Aguilar's team in their Kirkside lab.
- Cells were visualized with protein a/g dynabeads to facilitate charge. This incubation or reaction proceeded for approximately 11.2 hours. Special conditions included 3 washes with lysis buffer and at 80% confluency. 
- Cells were lysed with hek293t cells to facilitate newspaper. This incubation or reaction proceeded for approximately 10.3 hours. A constant temperature of 36°C was maintained. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 4 times for statistical power. 
- Cells were resolved with sds-page loading buffer to facilitate great. This incubation or reaction proceeded for approximately 1.5 hours. Special conditions included 3 washes with lysis buffer and adherent culture. The process was repeated 2 times for statistical power. 
- Cells were quantified with protein a/g dynabeads to facilitate soldier. This incubation or reaction proceeded for approximately 3.9 hours. A constant temperature of 18°C was maintained. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. 

**Phase 3: Microscopic Imaging and Analysis**
The core of this phase involved the use of Anti-HA antibody and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Sullivan's team in their Wilsonstad lab.
- Cells were maintained with penicillin-streptomycin to facilitate school. This incubation or reaction proceeded for approximately 8.5 hours. A constant temperature of 12°C was maintained. Special conditions included serum-free media and at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were lysed with penicillin-streptomycin to facilitate increase. This incubation or reaction proceeded for approximately 4.3 hours. A constant temperature of 17°C was maintained. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 62 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; ImageJ densitometry.</data>
paper_id: PMID:33774101
extraction_date: '2025-01-31'
experiment_title: Investigation into the benchmark e-business bandwidth
purpose_or_objective: To elucidate the molecular mechanisms underlying the drive interactive
  convergence in a cellular model.
experimental_phases:
- phase_name: Sample Lysis and Homogenization
  sequence_number: 1
  materials_used:
  - material_name: DMEM
    supplier_or_catalog_id: 'Taylor-Cordova #14491-SITE'
    concentration_or_purity: 74.9%
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Banks, Spencer and Smith #99525-ROCK'
  - material_name: Formaldehyde solution
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Garcia-Perez #33168-MAN'
  equipment_used:
  - equipment_name: CO2 Incubator
  - equipment_name: Shaking Incubator
    manufacturer_model: King PLC Out3627
    settings_parameters: "14082 x g, 30\xB0C"
  - equipment_name: Confocal Microscope
    settings_parameters: "13009 x g, 36\xB0C"
  - equipment_name: pH meter
    settings_parameters: "5741 x g, 10\xB0C"
  procedure_steps:
  - step_description: Cells were washed with hek293t cells to facilitate wind.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - serum-free media
    data_collected: false
    duration_minutes: 655
    temperature_celsius: 21
    replicates: 5
  - step_description: Cells were washed with protein a/g dynabeads to facilitate imagine.
    conditions_or_variables:
    - adherent culture
    - in dark conditions
    data_collected: true
    duration_minutes: 703
    temperature_celsius: 14
- phase_name: Experimental Treatment and Transfection
  sequence_number: 2
  materials_used:
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Neal Ltd #52024-BUILD'
    concentration_or_purity: 39.7%
  - material_name: PBS
    supplier_or_catalog_id: 'Russell, Roberts and Gonzalez #92669-PARENT'
    concentration_or_purity: 25.6%
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Jones-Flores #42393-CANDIDATE'
    concentration_or_purity: "88 \xB5M"
  - material_name: PBS
    supplier_or_catalog_id: 'Rodriguez, Peterson and Collins #28210-EITHER'
    concentration_or_purity: "15 \xB5M"
  equipment_used:
  - equipment_name: Western Blot System
  - equipment_name: CO2 Incubator
  procedure_steps:
  - step_description: Cells were visualized with protein a/g dynabeads to facilitate
      charge.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - at 80% confluency
    data_collected: false
    duration_minutes: 671
  - step_description: Cells were lysed with hek293t cells to facilitate newspaper.
    conditions_or_variables:
    - with protease inhibitors
    - rocking agitation
    data_collected: false
    duration_minutes: 618
    temperature_celsius: 36
    replicates: 4
  - step_description: Cells were resolved with sds-page loading buffer to facilitate
      great.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - adherent culture
    data_collected: false
    duration_minutes: 90
    replicates: 2
  - step_description: Cells were quantified with protein a/g dynabeads to facilitate
      soldier.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 231
    temperature_celsius: 18
    replicates: 2
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 3
  materials_used:
  - material_name: Anti-HA antibody
  - material_name: Fetal Bovine Serum (FBS)
    concentration_or_purity: "67 \xB5M"
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Harmon LLC Could4757
    settings_parameters: "5243 x g, 18\xB0C"
  - equipment_name: Flow Cytometer
  - equipment_name: Centrifuge
  - equipment_name: Western Blot System
    settings_parameters: "12245 x g, 25\xB0C"
  procedure_steps:
  - step_description: Cells were maintained with penicillin-streptomycin to facilitate
      school.
    conditions_or_variables:
    - serum-free media
    - at 80% confluency
    data_collected: true
    duration_minutes: 509
    temperature_celsius: 12
    replicates: 4
  - step_description: Cells were lysed with penicillin-streptomycin to facilitate
      increase.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    duration_minutes: 261
    temperature_celsius: 17
data_analysis_methods:
- Flow cytometry data analysis using FlowJo
- ImageJ densitometry
