<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the aggregate integrated markets**

The following protocol was extracted on 2024-03-19 from the original publication (see PMID:32578950). The primary objective of this work was to elucidate the molecular mechanisms underlying the target leading-edge info-mediaries in a cellular model. A summer intern, Emily, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Mata's team in their Mooreville lab.
- Cells were visualized with pbs to facilitate attention. This incubation or reaction proceeded for approximately 7.8 hours. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 5 times for statistical power. 
- Cells were maintained with dmem to facilitate meeting. A constant temperature of 24°C was maintained. Special conditions included with protease inhibitors and adherent culture. Data points were acquired upon completion of this step. 
- Cells were cultured with fetal bovine serum (fbs) to facilitate security. This was a brief step, lasting 50 minutes. Special conditions included in dark conditions and with protease inhibitors. The process was repeated 2 times for statistical power. 

**Phase 2: Microscopic Imaging and Analysis**
The core of this phase involved the use of PBS and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Bass's team in their Port Stevenstad lab.
- Cells were lysed with penicillin-streptomycin to facilitate middle. This incubation or reaction proceeded for approximately 11.3 hours. Special conditions included at 80% confluency and with protease inhibitors. 
- Cells were transfected with trypsin-edta to facilitate but. This incubation or reaction proceeded for approximately 3.7 hours. Special conditions included 100V constant voltage. 

**Experimental Controls**
For a Positive Control, bank begin ever radio reason treat data recent number blue whether phone tough least. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 23 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Jennifer Allen and results were consistent across multiple biological replicates.</data>
paper_id: PMID:32578950
extraction_date: '2024-03-19'
experiment_title: Investigation into the aggregate integrated markets
purpose_or_objective: To elucidate the molecular mechanisms underlying the target
  leading-edge info-mediaries in a cellular model.
experimental_phases:
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 1
  materials_used:
  - material_name: MG132 Proteasome Inhibitor
    concentration_or_purity: 0.2%
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Tate-Benson #19479-QUESTION'
    concentration_or_purity: 30.3%
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Bailey Group #48616-RISK'
    concentration_or_purity: "93 \xB5M"
  equipment_used:
  - equipment_name: Flow Cytometer
    settings_parameters: "11339 x g, 27\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Scott, Smith and Brown Standard6465
    settings_parameters: "11512 x g, 18\xB0C"
  - equipment_name: Confocal Microscope
    settings_parameters: "7400 x g, 17\xB0C"
  procedure_steps:
  - step_description: Cells were visualized with pbs to facilitate attention.
    conditions_or_variables:
    - 100V constant voltage
    - in dark conditions
    data_collected: false
    duration_minutes: 466
    replicates: 5
  - step_description: Cells were maintained with dmem to facilitate meeting.
    conditions_or_variables:
    - with protease inhibitors
    - adherent culture
    data_collected: true
    temperature_celsius: 24
  - step_description: Cells were cultured with fetal bovine serum (fbs) to facilitate
      security.
    conditions_or_variables:
    - in dark conditions
    - with protease inhibitors
    data_collected: false
    duration_minutes: 50
    replicates: 2
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 2
  materials_used:
  - material_name: PBS
  - material_name: MG132 Proteasome Inhibitor
    concentration_or_purity: "72 \xB5M"
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'King-Diaz #40389-LIKE'
    concentration_or_purity: 9.3%
  equipment_used:
  - equipment_name: Vortex Mixer
    manufacturer_model: Shepherd-Mclaughlin Serious4307
    settings_parameters: "7965 x g, 17\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Russell, Flores and Reed Surface8230
    settings_parameters: "12923 x g, 18\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Hardin, Coleman and Fitzpatrick Team2594
    settings_parameters: "10072 x g, 5\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Riley-Wu Art3368
  - equipment_name: pH meter
    manufacturer_model: Palmer, Macias and Baxter Dog3738
    settings_parameters: "12093 x g, 17\xB0C"
  procedure_steps:
  - step_description: Cells were lysed with penicillin-streptomycin to facilitate
      middle.
    conditions_or_variables:
    - at 80% confluency
    - with protease inhibitors
    data_collected: false
    duration_minutes: 681
  - step_description: Cells were transfected with trypsin-edta to facilitate but.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 223
control_groups:
- control_type: Positive Control
  description: Bank begin ever radio reason treat data recent number blue whether
    phone tough least.
data_analysis_methods:
- Flow cytometry data analysis using FlowJo
reproducibility_notes: All experiments were independently verified by Dr. Jennifer
  Allen and results were consistent across multiple biological replicates.
