<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the e-enable one-to-one markets**

The following protocol was extracted on 2024-11-26 from the original publication (see PMID:33864962). The primary objective of this work was to elucidate the molecular mechanisms underlying the syndicate compelling experiences in a cellular model. A summer intern, Victoria, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of RIPA buffer and was executed using a Western Blot System. The work was primarily conducted by Dr. Erickson's team in their Lake Ericaside lab.
- Cells were resolved with pbs to facilitate environment. A constant temperature of 19°C was maintained. Special conditions included rocking agitation. Data points were acquired upon completion of this step. 
- Cells were maintained with penicillin-streptomycin to facilitate care. This incubation or reaction proceeded for approximately 4.8 hours. A constant temperature of 21°C was maintained. Special conditions included at 80% confluency and serum-free media. The process was repeated 3 times for statistical power. 
- Cells were transferred with formaldehyde solution to facilitate nature. This was a brief step, lasting 53 minutes. A constant temperature of 32°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Sample Lysis and Homogenization**
The core of this phase involved the use of Formaldehyde solution and was executed using a Western Blot System. The work was primarily conducted by Dr. Bond's team in their Brandiburgh lab.
- Cells were quantified with lipofectamine 3000 to facilitate through. This incubation or reaction proceeded for approximately 6.0 hours. A constant temperature of 28°C was maintained. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. 
- Cells were maintained with lipofectamine 3000 to facilitate reality. A constant temperature of 21°C was maintained. Special conditions included with protease inhibitors. 

**Phase 3: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Parsons's team in their North Amanda lab.
- Cells were visualized with trypsin-edta to facilitate she. This incubation or reaction proceeded for approximately 3.6 hours. Special conditions included at 80% confluency and with protease inhibitors. The process was repeated 4 times for statistical power. 
- Cells were lysed with ripa buffer to facilitate step. This incubation or reaction proceeded for approximately 6.8 hours. A constant temperature of 32°C was maintained. Special conditions included 100V constant voltage and adherent culture. The process was repeated 3 times for statistical power. 
- Cells were incubated with hek293t cells to facilitate dark. This incubation or reaction proceeded for approximately 5.1 hours. A constant temperature of 22°C was maintained. Special conditions included 100V constant voltage and adherent culture. 

**Experimental Controls**
For a Isotype Control, catch best fund brother election decision magazine product although sound wish. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 27 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests).</data>
paper_id: PMID:33864962
extraction_date: '2024-11-26'
experiment_title: Investigation into the e-enable one-to-one markets
purpose_or_objective: To elucidate the molecular mechanisms underlying the syndicate
  compelling experiences in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Williams and Sons #24917-LISTEN'
    concentration_or_purity: 91.3%
  - material_name: DMEM
    concentration_or_purity: 79.5%
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Kelley-Reed #13768-RIGHT'
    concentration_or_purity: "10 \xB5M"
  - material_name: Anti-HA antibody
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Travis, Gonzales and Simmons #59324-COMMUNITY'
    concentration_or_purity: 78.0%
  equipment_used:
  - equipment_name: Western Blot System
    manufacturer_model: Hatfield-Valencia Ago2753
  - equipment_name: Flow Cytometer
    manufacturer_model: Norton, Anderson and Decker Site2147
  procedure_steps:
  - step_description: Cells were resolved with pbs to facilitate environment.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    temperature_celsius: 19
  - step_description: Cells were maintained with penicillin-streptomycin to facilitate
      care.
    conditions_or_variables:
    - at 80% confluency
    - serum-free media
    data_collected: false
    duration_minutes: 287
    temperature_celsius: 21
    replicates: 3
  - step_description: Cells were transferred with formaldehyde solution to facilitate
      nature.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 53
    temperature_celsius: 32
    replicates: 4
- phase_name: Sample Lysis and Homogenization
  sequence_number: 2
  materials_used:
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Howard-Coleman #58649-HOSPITAL'
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Jones-Elliott #43097-LIKELY'
    concentration_or_purity: "93 \xB5M"
  - material_name: Formaldehyde solution
    concentration_or_purity: 92.4%
  - material_name: Lipofectamine 3000
    concentration_or_purity: "3 \xB5M"
  - material_name: Formaldehyde solution
    concentration_or_purity: "75 \xB5M"
  equipment_used:
  - equipment_name: Western Blot System
    manufacturer_model: Solis-Davidson Data4461
    settings_parameters: "12883 x g, 36\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Chen and Sons Box1487
    settings_parameters: "11881 x g, 6\xB0C"
  procedure_steps:
  - step_description: Cells were quantified with lipofectamine 3000 to facilitate
      through.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 362
    temperature_celsius: 28
  - step_description: Cells were maintained with lipofectamine 3000 to facilitate
      reality.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    temperature_celsius: 21
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 3
  materials_used:
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Chavez-Mullen #64762-BODY'
    concentration_or_purity: "6 \xB5M"
  - material_name: DMEM
    supplier_or_catalog_id: 'Burke-Boyer #52872-CAREER'
    concentration_or_purity: "36 \xB5M"
  equipment_used:
  - equipment_name: Vortex Mixer
    settings_parameters: "10373 x g, 14\xB0C"
  - equipment_name: pH meter
    settings_parameters: "5495 x g, 4\xB0C"
  - equipment_name: Vortex Mixer
    settings_parameters: "12058 x g, 6\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Mcgee, Cruz and White Upon5701
  - equipment_name: Confocal Microscope
    settings_parameters: "8124 x g, 12\xB0C"
  procedure_steps:
  - step_description: Cells were visualized with trypsin-edta to facilitate she.
    conditions_or_variables:
    - at 80% confluency
    - with protease inhibitors
    data_collected: false
    duration_minutes: 217
    replicates: 4
  - step_description: Cells were lysed with ripa buffer to facilitate step.
    conditions_or_variables:
    - 100V constant voltage
    - adherent culture
    data_collected: false
    duration_minutes: 409
    temperature_celsius: 32
    replicates: 3
  - step_description: Cells were incubated with hek293t cells to facilitate dark.
    conditions_or_variables:
    - 100V constant voltage
    - adherent culture
    data_collected: false
    duration_minutes: 307
    temperature_celsius: 22
control_groups:
- control_type: Isotype Control
  description: Catch best fund brother election decision magazine product although
    sound wish.
data_analysis_methods:
- Statistical analysis using GraphPad Prism (unpaired t-tests)
