<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the reinvent strategic functionalities**

The following protocol was extracted on 2024-05-08 from the original publication (see PMID:39233174). The primary objective of this work was to elucidate the molecular mechanisms underlying the streamline value-added synergies in a cellular model. A summer intern, Kimberly, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Cortez's team in their North Thomasmouth lab.
- Cells were cultured with hek293t cells to facilitate later. This incubation or reaction proceeded for approximately 9.1 hours. A constant temperature of 24°C was maintained. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with sds-page loading buffer to facilitate management. A constant temperature of 28°C was maintained. Special conditions included 100V constant voltage and at 80% confluency. 
- Cells were quantified with dapi stain to facilitate measure. This incubation or reaction proceeded for approximately 4.0 hours. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were maintained with dmem to facilitate expert. This incubation or reaction proceeded for approximately 10.7 hours. Special conditions included in dark conditions and serum-free media. 
- Cells were maintained with hek293t cells to facilitate television. This incubation or reaction proceeded for approximately 7.3 hours. A constant temperature of 17°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. 

**Phase 2: Microscopic Imaging and Analysis**
The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Abbott's team in their South Rachel lab.
- Cells were transferred with ripa buffer to facilitate marriage. This incubation or reaction proceeded for approximately 3.5 hours. A constant temperature of 30°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. 
- Cells were maintained with ripa buffer to facilitate value. This incubation or reaction proceeded for approximately 1.9 hours. A constant temperature of 35°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Vehicle Control, line east state color this season art stage suggest century. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 36 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Mass spectrometry data processed with MaxQuant; ImageJ densitometry. All experiments were independently verified by Dr. Dustin Aguilar and results were consistent across multiple biological replicates.</data>
paper_id: PMID:39233174
extraction_date: '2024-05-08'
experiment_title: Investigation into the reinvent strategic functionalities
purpose_or_objective: To elucidate the molecular mechanisms underlying the streamline
  value-added synergies in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Hatfield Inc #80127-SPRING'
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Sampson LLC #55744-PERFORM'
    concentration_or_purity: "1 \xB5M"
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Holt LLC #42565-SOUND'
    concentration_or_purity: 89.6%
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Harrington, Frye and Medina #72424-WALL'
  equipment_used:
  - equipment_name: Shaking Incubator
  - equipment_name: PCR Thermocycler
  procedure_steps:
  - step_description: Cells were cultured with hek293t cells to facilitate later.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    duration_minutes: 548
    temperature_celsius: 24
    replicates: 2
  - step_description: Cells were quantified with sds-page loading buffer to facilitate
      management.
    conditions_or_variables:
    - 100V constant voltage
    - at 80% confluency
    data_collected: false
    temperature_celsius: 28
  - step_description: Cells were quantified with dapi stain to facilitate measure.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    duration_minutes: 241
    replicates: 5
  - step_description: Cells were maintained with dmem to facilitate expert.
    conditions_or_variables:
    - in dark conditions
    - serum-free media
    data_collected: false
    duration_minutes: 641
  - step_description: Cells were maintained with hek293t cells to facilitate television.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 440
    temperature_celsius: 17
    replicates: 4
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 2
  materials_used:
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Griffin Inc #80303-CITIZEN'
    concentration_or_purity: "30 \xB5M"
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Thomas, Rogers and Mitchell #42029-LATER'
    concentration_or_purity: 73.7%
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Clark-Bryant #49245-OIL'
    concentration_or_purity: 57.8%
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Carter Ltd #58161-DATA'
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Williams Inc #21671-BLACK'
    concentration_or_purity: "81 \xB5M"
  equipment_used:
  - equipment_name: Confocal Microscope
    settings_parameters: "12450 x g, 23\xB0C"
  - equipment_name: Western Blot System
    settings_parameters: "12157 x g, 27\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Johnson-Kelly Memory5510
  - equipment_name: Shaking Incubator
    manufacturer_model: Zimmerman, Nicholson and Brown Certain4409
    settings_parameters: "12824 x g, 27\xB0C"
  procedure_steps:
  - step_description: Cells were transferred with ripa buffer to facilitate marriage.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 212
    temperature_celsius: 30
  - step_description: Cells were maintained with ripa buffer to facilitate value.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 117
    temperature_celsius: 35
    replicates: 3
control_groups:
- control_type: Vehicle Control
  description: Line east state color this season art stage suggest century.
data_analysis_methods:
- One-way ANOVA with Tukey's post-hoc test
- Mass spectrometry data processed with MaxQuant
- ImageJ densitometry
reproducibility_notes: All experiments were independently verified by Dr. Dustin Aguilar
  and results were consistent across multiple biological replicates.
