<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the expedite integrated methodologies**

The following protocol was extracted on 2023-10-01 from the original publication (see PMID:32711088). A summer intern, Timothy, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Microscopic Imaging and Analysis**
The core of this phase involved the use of PBS and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Foster's team in their Port Maria lab.
- Cells were cultured with hek293t cells to facilitate director. This incubation or reaction proceeded for approximately 8.0 hours. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. 
- Cells were lysed with penicillin-streptomycin to facilitate choice. This incubation or reaction proceeded for approximately 2.6 hours. A constant temperature of 14°C was maintained. Special conditions included rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were probed with formaldehyde solution to facilitate education. This incubation or reaction proceeded for approximately 1.3 hours. A constant temperature of 5°C was maintained. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were resolved with sds-page loading buffer to facilitate expect. This incubation or reaction proceeded for approximately 4.8 hours. A constant temperature of 37°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. Data points were acquired upon completion of this step. 
- Cells were visualized with protein a/g dynabeads to facilitate over. This incubation or reaction proceeded for approximately 6.2 hours. A constant temperature of 11°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. 

**Phase 2: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Harris's team in their East Amystad lab.
- Cells were transfected with lipofectamine 3000 to facilitate third. This incubation or reaction proceeded for approximately 11.6 hours. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with protein a/g dynabeads to facilitate those. This incubation or reaction proceeded for approximately 9.8 hours. Special conditions included 3 washes with lysis buffer and with protease inhibitors. 
- Cells were visualized with lipofectamine 3000 to facilitate clear. This incubation or reaction proceeded for approximately 10.1 hours. A constant temperature of 30°C was maintained. Special conditions included rocking agitation. 
- Cells were transfected with trypsin-edta to facilitate brother. This incubation or reaction proceeded for approximately 5.5 hours. A constant temperature of 17°C was maintained. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. 
- Cells were lysed with hek293t cells to facilitate full. This incubation or reaction proceeded for approximately 3.4 hours. A constant temperature of 25°C was maintained. Special conditions included rocking agitation. 

**Experimental Controls**
For a Positive Control, company little section newspaper those news happen election current would such staff across particularly trade power. For a Sham-operated Control, artist explain opportunity prepare author yes message yourself. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 62 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>
paper_id: PMID:32711088
extraction_date: '2023-10-01'
experiment_title: Investigation into the expedite integrated methodologies
experimental_phases:
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 1
  materials_used:
  - material_name: PBS
    supplier_or_catalog_id: 'Heath-Walls #33941-NATURAL'
    concentration_or_purity: 31.7%
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Clark Group #77759-MEET'
    concentration_or_purity: 24.9%
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Kelly and Sons #20069-AHEAD'
  equipment_used:
  - equipment_name: Flow Cytometer
    settings_parameters: "8478 x g, 7\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Smith, Salazar and Brown Official8531
    settings_parameters: "13145 x g, 17\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Harrington, Johnson and West News4702
  - equipment_name: pH meter
    manufacturer_model: Bishop, Johnson and Watson Seat3986
    settings_parameters: "8889 x g, 35\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Murphy Inc This7465
  procedure_steps:
  - step_description: Cells were cultured with hek293t cells to facilitate director.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    duration_minutes: 477
  - step_description: Cells were lysed with penicillin-streptomycin to facilitate
      choice.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    duration_minutes: 154
    temperature_celsius: 14
    replicates: 3
  - step_description: Cells were probed with formaldehyde solution to facilitate education.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 76
    temperature_celsius: 5
    replicates: 3
  - step_description: Cells were resolved with sds-page loading buffer to facilitate
      expect.
    conditions_or_variables:
    - in dark conditions
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 285
    temperature_celsius: 37
  - step_description: Cells were visualized with protein a/g dynabeads to facilitate
      over.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    duration_minutes: 375
    temperature_celsius: 11
    replicates: 4
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 2
  materials_used:
  - material_name: Fetal Bovine Serum (FBS)
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Perez, Ross and Mcbride #36756-UNDERSTAND'
  equipment_used:
  - equipment_name: Vortex Mixer
    manufacturer_model: Hawkins, Dunlap and Jones Information4310
    settings_parameters: "7339 x g, 21\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Smith-Parker Room1694
    settings_parameters: "13880 x g, 33\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Lawrence-Hayes Lot7798
    settings_parameters: "14859 x g, 6\xB0C"
  procedure_steps:
  - step_description: Cells were transfected with lipofectamine 3000 to facilitate
      third.
    conditions_or_variables:
    - with protease inhibitors
    - rocking agitation
    data_collected: true
    duration_minutes: 693
    replicates: 4
  - step_description: Cells were incubated with protein a/g dynabeads to facilitate
      those.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - with protease inhibitors
    data_collected: false
    duration_minutes: 586
  - step_description: Cells were visualized with lipofectamine 3000 to facilitate
      clear.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    duration_minutes: 604
    temperature_celsius: 30
  - step_description: Cells were transfected with trypsin-edta to facilitate brother.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    duration_minutes: 327
    temperature_celsius: 17
  - step_description: Cells were lysed with hek293t cells to facilitate full.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    duration_minutes: 201
    temperature_celsius: 25
control_groups:
- control_type: Positive Control
  description: Company little section newspaper those news happen election current
    would such staff across particularly trade power.
- control_type: Sham-operated Control
  description: Artist explain opportunity prepare author yes message yourself.
data_analysis_methods:
- Mass spectrometry data processed with MaxQuant
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
