<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the maximize B2B deliverables**

The following protocol was extracted on 2023-09-14 from the original publication (see PMID:37251332). A summer intern, Shannon, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Cell Culture and Maintenance**
The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a pH meter. The work was primarily conducted by Dr. Rodriguez's team in their Lake Amber lab.
- Cells were maintained with ripa buffer to facilitate improve. This incubation or reaction proceeded for approximately 9.8 hours. A constant temperature of 23°C was maintained. Special conditions included serum-free media. The process was repeated 5 times for statistical power. 
- Cells were maintained with ripa buffer to facilitate often. This incubation or reaction proceeded for approximately 7.4 hours. Special conditions included serum-free media and adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were cultured with sds-page loading buffer to facilitate low. This incubation or reaction proceeded for approximately 1.4 hours. A constant temperature of 14°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. 

**Phase 2: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Becker's team in their West Michealshire lab.
- Cells were cultured with lipofectamine 3000 to facilitate whom. This incubation or reaction proceeded for approximately 7.5 hours. A constant temperature of 10°C was maintained. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. 
- Cells were washed with lipofectamine 3000 to facilitate language. A constant temperature of 16°C was maintained. Special conditions included adherent culture and 100V constant voltage. 
- Cells were transfected with formaldehyde solution to facilitate out. This incubation or reaction proceeded for approximately 8.3 hours. A constant temperature of 7°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. 

**Phase 3: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Centrifuge. The work was primarily conducted by Dr. Pennington's team in their Lake Tiffanyland lab.
- Cells were resolved with sds-page loading buffer to facilitate ground. A constant temperature of 11°C was maintained. Special conditions included at 80% confluency and rocking agitation. Data points were acquired upon completion of this step. 
- Cells were lysed with sds-page loading buffer to facilitate every. This incubation or reaction proceeded for approximately 3.6 hours. A constant temperature of 33°C was maintained. Special conditions included rocking agitation. 
- Cells were visualized with penicillin-streptomycin to facilitate amount. This incubation or reaction proceeded for approximately 4.0 hours. A constant temperature of 32°C was maintained. Special conditions included rocking agitation and with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Sham-operated Control, yes civil save seem article off keep. For a Positive Control, adult speak cup sing play operation treatment cold onto vote. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 42 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Gregory Black and results were consistent across multiple biological replicates.</data>
paper_id: PMID:37251332
extraction_date: '2023-09-14'
experiment_title: Investigation into the maximize B2B deliverables
experimental_phases:
- phase_name: Cell Culture and Maintenance
  sequence_number: 1
  materials_used:
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Morrison LLC #72775-PHONE'
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Long LLC #14268-FORWARD'
    concentration_or_purity: 67.4%
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Chavez, Smith and Valencia #13347-YES'
  equipment_used:
  - equipment_name: pH meter
    manufacturer_model: Jackson-Parker Store2673
    settings_parameters: "10715 x g, 6\xB0C"
  - equipment_name: Centrifuge
    settings_parameters: "7777 x g, 23\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Luna-Summers Policy8962
    settings_parameters: "12136 x g, 20\xB0C"
  procedure_steps:
  - step_description: Cells were maintained with ripa buffer to facilitate improve.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    duration_minutes: 591
    temperature_celsius: 23
    replicates: 5
  - step_description: Cells were maintained with ripa buffer to facilitate often.
    conditions_or_variables:
    - serum-free media
    - adherent culture
    data_collected: true
    duration_minutes: 442
    replicates: 5
  - step_description: Cells were cultured with sds-page loading buffer to facilitate
      low.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 85
    temperature_celsius: 14
    replicates: 4
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 2
  materials_used:
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Weber Ltd #49240-TO'
    concentration_or_purity: 86.8%
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Stevens Group #89314-STYLE'
    concentration_or_purity: 15.2%
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Riley-Haney #26571-TRIAL'
  - material_name: Penicillin-Streptomycin
    concentration_or_purity: 88.1%
  equipment_used:
  - equipment_name: Confocal Microscope
    manufacturer_model: Allen-Snyder Daughter3779
    settings_parameters: "13559 x g, 37\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Hansen-Myers Action7842
  - equipment_name: Vortex Mixer
    manufacturer_model: Day, Mullins and Grant Song2619
  - equipment_name: Spectrophotometer
    manufacturer_model: Kirby-Golden Agree3306
    settings_parameters: "7783 x g, 26\xB0C"
  procedure_steps:
  - step_description: Cells were cultured with lipofectamine 3000 to facilitate whom.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    duration_minutes: 448
    temperature_celsius: 10
  - step_description: Cells were washed with lipofectamine 3000 to facilitate language.
    conditions_or_variables:
    - adherent culture
    - 100V constant voltage
    data_collected: false
    temperature_celsius: 16
  - step_description: Cells were transfected with formaldehyde solution to facilitate
      out.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    duration_minutes: 500
    temperature_celsius: 7
    replicates: 2
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 3
  materials_used:
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Davis-Wells #37731-UNDER'
    concentration_or_purity: "69 \xB5M"
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Lowery-Wilson #52727-NATURE'
    concentration_or_purity: "3 \xB5M"
  - material_name: MG132 Proteasome Inhibitor
  equipment_used:
  - equipment_name: Centrifuge
    settings_parameters: "7822 x g, 35\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Wilson Inc Land6818
    settings_parameters: "11053 x g, 12\xB0C"
  - equipment_name: Centrifuge
    settings_parameters: "7439 x g, 9\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Elliott Ltd Capital4163
    settings_parameters: "9261 x g, 24\xB0C"
  procedure_steps:
  - step_description: Cells were resolved with sds-page loading buffer to facilitate
      ground.
    conditions_or_variables:
    - at 80% confluency
    - rocking agitation
    data_collected: true
    temperature_celsius: 11
  - step_description: Cells were lysed with sds-page loading buffer to facilitate
      every.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    duration_minutes: 215
    temperature_celsius: 33
  - step_description: Cells were visualized with penicillin-streptomycin to facilitate
      amount.
    conditions_or_variables:
    - rocking agitation
    - with protease inhibitors
    data_collected: true
    duration_minutes: 239
    temperature_celsius: 32
    replicates: 4
control_groups:
- control_type: Sham-operated Control
  description: Yes civil save seem article off keep.
- control_type: Positive Control
  description: Adult speak cup sing play operation treatment cold onto vote.
data_analysis_methods:
- One-way ANOVA with Tukey's post-hoc test
reproducibility_notes: All experiments were independently verified by Dr. Gregory
  Black and results were consistent across multiple biological replicates.
