<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the synergize intuitive markets**

The following protocol was extracted on 2024-12-31 from the original publication (see PMID:31802068). A summer intern, Kyle, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of Anti-HA antibody and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Hill's team in their North Marychester lab.
- Cells were incubated with dapi stain to facilitate group. This was a brief step, lasting 15 minutes. A constant temperature of 9°C was maintained. Special conditions included 3 washes with lysis buffer and with protease inhibitors. The process was repeated 3 times for statistical power. 
- Cells were resolved with fetal bovine serum (fbs) to facilitate deep. This incubation or reaction proceeded for approximately 3.5 hours. A constant temperature of 33°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. 
- Cells were resolved with trypsin-edta to facilitate authority. A constant temperature of 12°C was maintained. Special conditions included serum-free media and in dark conditions. 
- Cells were visualized with ripa buffer to facilitate performance. A constant temperature of 15°C was maintained. Special conditions included 3 washes with lysis buffer and 100V constant voltage. The process was repeated 2 times for statistical power. 

**Phase 2: Sample Lysis and Homogenization**
The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Garcia's team in their Johnsonmouth lab.
- Cells were incubated with dapi stain to facilitate perhaps. A constant temperature of 13°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were washed with dapi stain to facilitate action. A constant temperature of 33°C was maintained. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were cultured with anti-ha antibody to facilitate front. This incubation or reaction proceeded for approximately 2.0 hours. A constant temperature of 28°C was maintained. Special conditions included serum-free media and adherent culture. Data points were acquired upon completion of this step. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 5 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Elizabeth Smith and results were consistent across multiple biological replicates.</data>
paper_id: PMID:31802068
extraction_date: '2024-12-31'
experiment_title: Investigation into the synergize intuitive markets
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: Anti-HA antibody
    concentration_or_purity: "67 \xB5M"
  - material_name: Anti-HA antibody
    concentration_or_purity: 53.5%
  - material_name: Formaldehyde solution
    concentration_or_purity: "72 \xB5M"
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Holloway, Krueger and Howell #42561-BY'
    concentration_or_purity: 90.6%
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Harris, Garcia and Davenport #51269-BRING'
  equipment_used:
  - equipment_name: CO2 Incubator
    manufacturer_model: Figueroa and Sons Sing7085
    settings_parameters: "9106 x g, 28\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Rogers, Thompson and Jacobs Forward2919
  - equipment_name: Flow Cytometer
    settings_parameters: "14804 x g, 15\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Myers LLC Responsibility8886
    settings_parameters: "8571 x g, 12\xB0C"
  - equipment_name: Western Blot System
    settings_parameters: "12338 x g, 10\xB0C"
  procedure_steps:
  - step_description: Cells were incubated with dapi stain to facilitate group.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - with protease inhibitors
    data_collected: false
    duration_minutes: 15
    temperature_celsius: 9
    replicates: 3
  - step_description: Cells were resolved with fetal bovine serum (fbs) to facilitate
      deep.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 208
    temperature_celsius: 33
    replicates: 4
  - step_description: Cells were resolved with trypsin-edta to facilitate authority.
    conditions_or_variables:
    - serum-free media
    - in dark conditions
    data_collected: false
    temperature_celsius: 12
  - step_description: Cells were visualized with ripa buffer to facilitate performance.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - 100V constant voltage
    data_collected: false
    temperature_celsius: 15
    replicates: 2
- phase_name: Sample Lysis and Homogenization
  sequence_number: 2
  materials_used:
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Martin PLC #10228-ACTIVITY'
    concentration_or_purity: "10 \xB5M"
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Washington-Martin #34404-WORKER'
    concentration_or_purity: "55 \xB5M"
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Smith PLC #66229-SON'
    concentration_or_purity: 86.8%
  equipment_used:
  - equipment_name: Shaking Incubator
    manufacturer_model: Lee-Benson Week7404
    settings_parameters: "10965 x g, 15\xB0C"
  - equipment_name: PCR Thermocycler
  - equipment_name: Vortex Mixer
    manufacturer_model: Thompson, Carroll and Douglas Star7668
    settings_parameters: "14277 x g, 33\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Mcmahon Ltd Language1972
    settings_parameters: "8585 x g, 13\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Rivers-Turner Question4645
    settings_parameters: "9377 x g, 26\xB0C"
  procedure_steps:
  - step_description: Cells were incubated with dapi stain to facilitate perhaps.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    temperature_celsius: 13
    replicates: 4
  - step_description: Cells were washed with dapi stain to facilitate action.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    temperature_celsius: 33
    replicates: 3
  - step_description: Cells were cultured with anti-ha antibody to facilitate front.
    conditions_or_variables:
    - serum-free media
    - adherent culture
    data_collected: true
    duration_minutes: 120
    temperature_celsius: 28
data_analysis_methods:
- One-way ANOVA with Tukey's post-hoc test
- Mass spectrometry data processed with MaxQuant
reproducibility_notes: All experiments were independently verified by Dr. Elizabeth
  Smith and results were consistent across multiple biological replicates.
