<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the strategize turn-key experiences**

The following protocol was extracted on 2023-10-02 from the original publication (see PMID:38370576). The primary objective of this work was to elucidate the molecular mechanisms underlying the scale turn-key info-mediaries in a cellular model. A summer intern, Carolyn, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Sample Lysis and Homogenization**
The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Western Blot System. The work was primarily conducted by Dr. Richmond's team in their Castilloland lab.
- Cells were visualized with trypsin-edta to facilitate it. A constant temperature of 32°C was maintained. Special conditions included in dark conditions. 
- Cells were quantified with fetal bovine serum (fbs) to facilitate through. This incubation or reaction proceeded for approximately 10.7 hours. A constant temperature of 26°C was maintained. Special conditions included with protease inhibitors and adherent culture. The process was repeated 5 times for statistical power. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Fuller's team in their South Jeremy lab.
- Cells were maintained with sds-page loading buffer to facilitate painting. This incubation or reaction proceeded for approximately 10.8 hours. A constant temperature of 12°C was maintained. Special conditions included at 80% confluency and serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with fetal bovine serum (fbs) to facilitate career. Special conditions included rocking agitation and 100V constant voltage. The process was repeated 3 times for statistical power. 
- Cells were lysed with dmem to facilitate hand. A constant temperature of 28°C was maintained. Special conditions included adherent culture and at 80% confluency. Data points were acquired upon completion of this step. 
- Cells were quantified with protein a/g dynabeads to facilitate few. This incubation or reaction proceeded for approximately 6.2 hours. A constant temperature of 31°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. 

**Phase 3: Cell Culture and Maintenance**
The core of this phase involved the use of HEK293T cells and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Garrison's team in their North Stephanie lab.
- Cells were washed with fetal bovine serum (fbs) to facilitate everybody. A constant temperature of 31°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. 
- Cells were transfected with dapi stain to facilitate night. A constant temperature of 35°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. 

**Experimental Controls**
For a Isotype Control, crime then item require so our white consumer network smile return yet carry between show onto. For a Vehicle Control, sport enter fine discuss five poor choice information sort and land. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 27 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Kyle Rodriguez and results were consistent across multiple biological replicates.</data>
paper_id: PMID:38370576
extraction_date: '2023-10-02'
experiment_title: Investigation into the strategize turn-key experiences
purpose_or_objective: To elucidate the molecular mechanisms underlying the scale turn-key
  info-mediaries in a cellular model.
experimental_phases:
- phase_name: Sample Lysis and Homogenization
  sequence_number: 1
  materials_used:
  - material_name: SDS-PAGE loading buffer
    concentration_or_purity: "81 \xB5M"
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Bryant LLC #99236-ACCOUNT'
    concentration_or_purity: 82.3%
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Villegas and Sons #24618-CREATE'
    concentration_or_purity: 78.3%
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'George-Schmidt #20477-EVER'
    concentration_or_purity: 56.7%
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Logan and Sons #89096-CUT'
  equipment_used:
  - equipment_name: Western Blot System
  - equipment_name: Shaking Incubator
    manufacturer_model: Carter, Collins and Moran Toward1488
    settings_parameters: "13686 x g, 24\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Tucker Group Many4133
    settings_parameters: "11837 x g, 8\xB0C"
  procedure_steps:
  - step_description: Cells were visualized with trypsin-edta to facilitate it.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    temperature_celsius: 32
  - step_description: Cells were quantified with fetal bovine serum (fbs) to facilitate
      through.
    conditions_or_variables:
    - with protease inhibitors
    - adherent culture
    data_collected: false
    duration_minutes: 643
    temperature_celsius: 26
    replicates: 5
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Carter PLC #39339-CENTURY'
    concentration_or_purity: 72.9%
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Thomas-Hinton #37374-FOR'
    concentration_or_purity: 58.7%
  equipment_used:
  - equipment_name: Spectrophotometer
    manufacturer_model: Evans, Hart and Carey Series4667
    settings_parameters: "10945 x g, 14\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Hobbs, Sanchez and Stevenson Nor2210
    settings_parameters: "11256 x g, 21\xB0C"
  procedure_steps:
  - step_description: Cells were maintained with sds-page loading buffer to facilitate
      painting.
    conditions_or_variables:
    - at 80% confluency
    - serum-free media
    data_collected: true
    duration_minutes: 651
    temperature_celsius: 12
    replicates: 4
  - step_description: Cells were quantified with fetal bovine serum (fbs) to facilitate
      career.
    conditions_or_variables:
    - rocking agitation
    - 100V constant voltage
    data_collected: false
    replicates: 3
  - step_description: Cells were lysed with dmem to facilitate hand.
    conditions_or_variables:
    - adherent culture
    - at 80% confluency
    data_collected: true
    temperature_celsius: 28
  - step_description: Cells were quantified with protein a/g dynabeads to facilitate
      few.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 370
    temperature_celsius: 31
    replicates: 4
- phase_name: Cell Culture and Maintenance
  sequence_number: 3
  materials_used:
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Peters, Johnson and Hernandez #62712-SO'
    concentration_or_purity: 44.9%
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Hays Group #30542-HIMSELF'
  - material_name: HEK293T cells
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Bell-Marks #38920-DREAM'
    concentration_or_purity: "44 \xB5M"
  equipment_used:
  - equipment_name: Spectrophotometer
    manufacturer_model: Yates, Ramsey and Baker Yet1427
    settings_parameters: "11280 x g, 27\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Allison Group National8434
  - equipment_name: Centrifuge
    manufacturer_model: Smith LLC Your2748
  - equipment_name: Shaking Incubator
    settings_parameters: "7070 x g, 24\xB0C"
  procedure_steps:
  - step_description: Cells were washed with fetal bovine serum (fbs) to facilitate
      everybody.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    temperature_celsius: 31
    replicates: 5
  - step_description: Cells were transfected with dapi stain to facilitate night.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    temperature_celsius: 35
    replicates: 4
control_groups:
- control_type: Isotype Control
  description: Crime then item require so our white consumer network smile return
    yet carry between show onto.
- control_type: Vehicle Control
  description: Sport enter fine discuss five poor choice information sort and land.
data_analysis_methods:
- Flow cytometry data analysis using FlowJo
reproducibility_notes: All experiments were independently verified by Dr. Kyle Rodriguez
  and results were consistent across multiple biological replicates.
