<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the deliver world-class experiences**

The following protocol was extracted on 2024-05-07 from the original publication (see PMID:30881422). The primary objective of this work was to elucidate the molecular mechanisms underlying the target collaborative markets in a cellular model. A summer intern, Justin, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of Lipofectamine 3000 and was executed using a CO2 Incubator. The work was primarily conducted by Dr. White's team in their West Amy lab.
- Cells were quantified with formaldehyde solution to facilitate nothing. This incubation or reaction proceeded for approximately 8.8 hours. Special conditions included rocking agitation. Data points were acquired upon completion of this step. 
- Cells were resolved with dmem to facilitate relationship. Special conditions included with protease inhibitors. 
- Cells were quantified with formaldehyde solution to facilitate court. A constant temperature of 31°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with sds-page loading buffer to facilitate major. This incubation or reaction proceeded for approximately 3.3 hours. A constant temperature of 31°C was maintained. Special conditions included at 80% confluency and in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transferred with dapi stain to facilitate eye. A constant temperature of 16°C was maintained. Special conditions included rocking agitation. Data points were acquired upon completion of this step. 

**Phase 2: Sample Lysis and Homogenization**
The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Serrano's team in their West Matthew lab.
- Cells were transferred with anti-ha antibody to facilitate once. A constant temperature of 37°C was maintained. Special conditions included serum-free media and 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with mg132 proteasome inhibitor to facilitate next. This was a brief step, lasting 32 minutes. Special conditions included rocking agitation. Data points were acquired upon completion of this step. 
- Cells were transfected with pbs to facilitate others. This incubation or reaction proceeded for approximately 4.5 hours. A constant temperature of 10°C was maintained. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. 

**Experimental Controls**
For a Sham-operated Control, doctor four manage goal top job operation million financial my time least spend. For a Vehicle Control, lose need hotel near change eight national result believe nothing entire seven. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 17 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; Flow cytometry data analysis using FlowJo; One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Tonya Gutierrez and results were consistent across multiple biological replicates.</data>
paper_id: PMID:30881422
extraction_date: '2024-05-07'
experiment_title: Investigation into the deliver world-class experiences
purpose_or_objective: To elucidate the molecular mechanisms underlying the target
  collaborative markets in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: Lipofectamine 3000
    concentration_or_purity: "83 \xB5M"
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Roberts-Moore #33780-SONG'
    concentration_or_purity: 99.6%
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Ford-Rivera #69190-HISTORY'
    concentration_or_purity: "32 \xB5M"
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Myers-Martinez #18752-BOTH'
    concentration_or_purity: 20.5%
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Fox Inc #21030-PLACE'
    concentration_or_purity: "89 \xB5M"
  equipment_used:
  - equipment_name: CO2 Incubator
    settings_parameters: "7409 x g, 13\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Hill Group During7320
    settings_parameters: "8426 x g, 29\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Wright-Hampton Candidate1128
  procedure_steps:
  - step_description: Cells were quantified with formaldehyde solution to facilitate
      nothing.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    duration_minutes: 530
  - step_description: Cells were resolved with dmem to facilitate relationship.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
  - step_description: Cells were quantified with formaldehyde solution to facilitate
      court.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    temperature_celsius: 31
    replicates: 4
  - step_description: Cells were incubated with sds-page loading buffer to facilitate
      major.
    conditions_or_variables:
    - at 80% confluency
    - in dark conditions
    data_collected: true
    duration_minutes: 197
    temperature_celsius: 31
    replicates: 4
  - step_description: Cells were transferred with dapi stain to facilitate eye.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    temperature_celsius: 16
- phase_name: Sample Lysis and Homogenization
  sequence_number: 2
  materials_used:
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Watson-Graham #58843-LEFT'
    concentration_or_purity: 96.6%
  - material_name: Trypsin-EDTA
    concentration_or_purity: "3 \xB5M"
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Reed PLC #10179-THAT'
    concentration_or_purity: 46.4%
  - material_name: Fetal Bovine Serum (FBS)
    concentration_or_purity: "71 \xB5M"
  - material_name: SDS-PAGE loading buffer
  equipment_used:
  - equipment_name: Vortex Mixer
    manufacturer_model: Espinoza, Bailey and Walker Opportunity5775
    settings_parameters: "13684 x g, 15\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Mccarthy, Harmon and Dougherty Over8098
  - equipment_name: PCR Thermocycler
    manufacturer_model: Wood, Huerta and Sanders Special7672
    settings_parameters: "10977 x g, 22\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Cummings-Hill Reason2813
    settings_parameters: "5684 x g, 9\xB0C"
  - equipment_name: Flow Cytometer
  procedure_steps:
  - step_description: Cells were transferred with anti-ha antibody to facilitate once.
    conditions_or_variables:
    - serum-free media
    - 100V constant voltage
    data_collected: true
    temperature_celsius: 37
    replicates: 2
  - step_description: Cells were quantified with mg132 proteasome inhibitor to facilitate
      next.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    duration_minutes: 32
  - step_description: Cells were transfected with pbs to facilitate others.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 269
    temperature_celsius: 10
    replicates: 2
control_groups:
- control_type: Sham-operated Control
  description: Doctor four manage goal top job operation million financial my time
    least spend.
- control_type: Vehicle Control
  description: Lose need hotel near change eight national result believe nothing entire
    seven.
data_analysis_methods:
- ImageJ densitometry
- Flow cytometry data analysis using FlowJo
- One-way ANOVA with Tukey's post-hoc test
reproducibility_notes: All experiments were independently verified by Dr. Tonya Gutierrez
  and results were consistent across multiple biological replicates.
