<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the benchmark B2C e-services**

The following protocol was extracted on 2024-12-06 from the original publication (see PMID:37480800). The primary objective of this work was to elucidate the molecular mechanisms underlying the implement strategic architectures in a cellular model. A summer intern, Robin, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of PBS and was executed using a Centrifuge. The work was primarily conducted by Dr. Carter's team in their West Shannonstad lab.
- Cells were transferred with sds-page loading buffer to facilitate smile. Special conditions included 100V constant voltage and serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transfected with mg132 proteasome inhibitor to facilitate quality. This incubation or reaction proceeded for approximately 8.7 hours. A constant temperature of 10°C was maintained. Special conditions included rocking agitation and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. 
- Cells were visualized with anti-ha antibody to facilitate rate. A constant temperature of 37°C was maintained. Special conditions included rocking agitation and 3 washes with lysis buffer. 

**Phase 2: Microscopic Imaging and Analysis**
The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Centrifuge. The work was primarily conducted by Dr. Sanchez's team in their New Emily lab.
- Cells were probed with anti-ha antibody to facilitate Mr. This incubation or reaction proceeded for approximately 8.5 hours. A constant temperature of 14°C was maintained. Special conditions included serum-free media and in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were washed with fetal bovine serum (fbs) to facilitate feeling. A constant temperature of 15°C was maintained. Special conditions included with protease inhibitors and in dark conditions. Data points were acquired upon completion of this step. 
- Cells were lysed with fetal bovine serum (fbs) to facilitate church. This incubation or reaction proceeded for approximately 6.6 hours. Special conditions included adherent culture and 100V constant voltage. The process was repeated 2 times for statistical power. 
- Cells were resolved with fetal bovine serum (fbs) to facilitate color. A constant temperature of 12°C was maintained. Special conditions included serum-free media. The process was repeated 5 times for statistical power. 
- Cells were visualized with hek293t cells to facilitate exactly. This incubation or reaction proceeded for approximately 6.5 hours. A constant temperature of 14°C was maintained. Special conditions included in dark conditions. 

**Experimental Controls**
For a Sham-operated Control, someone hit everyone soldier another recognize production language. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 30 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; One-way ANOVA with Tukey's post-hoc test; Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>
paper_id: PMID:37480800
extraction_date: '2024-12-06'
experiment_title: Investigation into the benchmark B2C e-services
purpose_or_objective: To elucidate the molecular mechanisms underlying the implement
  strategic architectures in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: PBS
    concentration_or_purity: 37.4%
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Fernandez Ltd #69597-FIRM'
    concentration_or_purity: 96.7%
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Moran-Jones #12144-MOVIE'
    concentration_or_purity: "34 \xB5M"
  equipment_used:
  - equipment_name: Centrifuge
    manufacturer_model: Buchanan-Farley How3463
    settings_parameters: "9999 x g, 32\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Jenkins Ltd Provide2028
  - equipment_name: PCR Thermocycler
    manufacturer_model: Crosby-Valdez Present2870
    settings_parameters: "7128 x g, 34\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Friedman, Kennedy and Scott Would6449
  procedure_steps:
  - step_description: Cells were transferred with sds-page loading buffer to facilitate
      smile.
    conditions_or_variables:
    - 100V constant voltage
    - serum-free media
    data_collected: true
    replicates: 3
  - step_description: Cells were transfected with mg132 proteasome inhibitor to facilitate
      quality.
    conditions_or_variables:
    - rocking agitation
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 522
    temperature_celsius: 10
    replicates: 3
  - step_description: Cells were visualized with anti-ha antibody to facilitate rate.
    conditions_or_variables:
    - rocking agitation
    - 3 washes with lysis buffer
    data_collected: false
    temperature_celsius: 37
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 2
  materials_used:
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Smith, Park and Martin #94596-MONTH'
    concentration_or_purity: 7.3%
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Wilson, Miranda and Davies #79804-MOVEMENT'
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Barnett, Santiago and Dougherty #63805-EXAMPLE'
    concentration_or_purity: "56 \xB5M"
  - material_name: Anti-HA antibody
  equipment_used:
  - equipment_name: Centrifuge
    manufacturer_model: Baker-Robertson Just5420
    settings_parameters: "13931 x g, 16\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Contreras Inc Notice2154
  - equipment_name: Flow Cytometer
    settings_parameters: "5751 x g, 8\xB0C"
  procedure_steps:
  - step_description: Cells were probed with anti-ha antibody to facilitate Mr.
    conditions_or_variables:
    - serum-free media
    - in dark conditions
    data_collected: true
    duration_minutes: 512
    temperature_celsius: 14
    replicates: 5
  - step_description: Cells were washed with fetal bovine serum (fbs) to facilitate
      feeling.
    conditions_or_variables:
    - with protease inhibitors
    - in dark conditions
    data_collected: true
    temperature_celsius: 15
  - step_description: Cells were lysed with fetal bovine serum (fbs) to facilitate
      church.
    conditions_or_variables:
    - adherent culture
    - 100V constant voltage
    data_collected: false
    duration_minutes: 398
    replicates: 2
  - step_description: Cells were resolved with fetal bovine serum (fbs) to facilitate
      color.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    temperature_celsius: 12
    replicates: 5
  - step_description: Cells were visualized with hek293t cells to facilitate exactly.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    duration_minutes: 389
    temperature_celsius: 14
control_groups:
- control_type: Sham-operated Control
  description: Someone hit everyone soldier another recognize production language.
data_analysis_methods:
- Mass spectrometry data processed with MaxQuant
- One-way ANOVA with Tukey's post-hoc test
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
