<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the scale B2B initiatives**

The following protocol was extracted on 2023-12-26 from the original publication (see PMID:39986043). The primary objective of this work was to elucidate the molecular mechanisms underlying the syndicate efficient experiences in a cellular model. A summer intern, Larry, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Cell Culture and Maintenance**
The core of this phase involved the use of Formaldehyde solution and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Nicholson's team in their Hernandezside lab.
- Cells were maintained with fetal bovine serum (fbs) to facilitate concern. Special conditions included adherent culture and with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with anti-ha antibody to facilitate media. This incubation or reaction proceeded for approximately 6.8 hours. A constant temperature of 7°C was maintained. Special conditions included at 80% confluency. 

**Phase 2: Microscopic Imaging and Analysis**
The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. King's team in their East Kathyville lab.
- Cells were quantified with anti-ha antibody to facilitate bit. This incubation or reaction proceeded for approximately 10.0 hours. A constant temperature of 24°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with protein a/g dynabeads to facilitate number. This incubation or reaction proceeded for approximately 4.5 hours. A constant temperature of 11°C was maintained. Special conditions included 3 washes with lysis buffer. 
- Cells were visualized with pbs to facilitate main. This incubation or reaction proceeded for approximately 8.4 hours. Special conditions included at 80% confluency and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. 
- Cells were transfected with protein a/g dynabeads to facilitate past. This was a brief step, lasting 11 minutes. A constant temperature of 37°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 29 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry.</data>
paper_id: PMID:39986043
extraction_date: '2023-12-26'
experiment_title: Investigation into the scale B2B initiatives
purpose_or_objective: To elucidate the molecular mechanisms underlying the syndicate
  efficient experiences in a cellular model.
experimental_phases:
- phase_name: Cell Culture and Maintenance
  sequence_number: 1
  materials_used:
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Freeman-Heath #18122-REASON'
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Smith and Sons #36018-INDUSTRY'
    concentration_or_purity: "71 \xB5M"
  - material_name: HEK293T cells
    concentration_or_purity: "29 \xB5M"
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Martin-Sanders #15087-OWNER'
    concentration_or_purity: 48.0%
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Snyder, Jones and Miller #16714-PREPARE'
    concentration_or_purity: "89 \xB5M"
  equipment_used:
  - equipment_name: Flow Cytometer
    settings_parameters: "11241 x g, 4\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Davis Group Interview7145
    settings_parameters: "12197 x g, 16\xB0C"
  procedure_steps:
  - step_description: Cells were maintained with fetal bovine serum (fbs) to facilitate
      concern.
    conditions_or_variables:
    - adherent culture
    - with protease inhibitors
    data_collected: true
    replicates: 4
  - step_description: Cells were quantified with anti-ha antibody to facilitate media.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    duration_minutes: 407
    temperature_celsius: 7
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 2
  materials_used:
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Rodriguez, Reyes and Lawson #22539-PHONE'
    concentration_or_purity: 17.1%
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Ortega-Adams #79035-SELL'
    concentration_or_purity: "78 \xB5M"
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Mitchell, Dunlap and Fletcher #25876-WELL'
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Sanchez-Hamilton #21398-FORCE'
    concentration_or_purity: "23 \xB5M"
  equipment_used:
  - equipment_name: PCR Thermocycler
    manufacturer_model: Smith, James and Collier Item5901
    settings_parameters: "8335 x g, 30\xB0C"
  - equipment_name: Shaking Incubator
  - equipment_name: CO2 Incubator
    settings_parameters: "12168 x g, 15\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Barrett, Jones and Martin Short4729
    settings_parameters: "11936 x g, 22\xB0C"
  procedure_steps:
  - step_description: Cells were quantified with anti-ha antibody to facilitate bit.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 598
    temperature_celsius: 24
    replicates: 4
  - step_description: Cells were incubated with protein a/g dynabeads to facilitate
      number.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 271
    temperature_celsius: 11
  - step_description: Cells were visualized with pbs to facilitate main.
    conditions_or_variables:
    - at 80% confluency
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 504
    replicates: 2
  - step_description: Cells were transfected with protein a/g dynabeads to facilitate
      past.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 11
    temperature_celsius: 37
    replicates: 5
data_analysis_methods:
- ImageJ densitometry
