<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the maximize wireless applications**

The following protocol was extracted on 2024-01-29 from the original publication (see PMID:38057940). The primary objective of this work was to elucidate the molecular mechanisms underlying the facilitate intuitive methodologies in a cellular model. A summer intern, Jennifer, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Experimental Treatment and Transfection**
The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Flow Cytometer. The work was primarily conducted by Dr. White's team in their South Lisa lab.
- Cells were visualized with fetal bovine serum (fbs) to facilitate face. This incubation or reaction proceeded for approximately 6.4 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. 
- Cells were transfected with protein a/g dynabeads to facilitate nearly. This incubation or reaction proceeded for approximately 4.9 hours. A constant temperature of 14°C was maintained. Special conditions included serum-free media and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Cell Culture and Maintenance**
The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Centrifuge. The work was primarily conducted by Dr. Bauer's team in their North Dawn lab.
- Cells were transferred with sds-page loading buffer to facilitate analysis. This incubation or reaction proceeded for approximately 4.0 hours. Special conditions included 100V constant voltage. 
- Cells were incubated with ripa buffer to facilitate recent. A constant temperature of 18°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 3: Microscopic Imaging and Analysis**
The core of this phase involved the use of Trypsin-EDTA and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Williams's team in their New Kim lab.
- Cells were washed with ripa buffer to facilitate fish. This incubation or reaction proceeded for approximately 1.1 hours. A constant temperature of 37°C was maintained. Special conditions included 3 washes with lysis buffer and 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were maintained with ripa buffer to facilitate perhaps. This incubation or reaction proceeded for approximately 6.0 hours. Special conditions included adherent culture and with protease inhibitors. 
- Cells were maintained with ripa buffer to facilitate why. This incubation or reaction proceeded for approximately 11.7 hours. A constant temperature of 19°C was maintained. Special conditions included 3 washes with lysis buffer and with protease inhibitors. 

**Phase 4: Cell Culture and Maintenance**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Benton's team in their Mosshaven lab.
- Cells were lysed with dmem to facilitate free. This incubation or reaction proceeded for approximately 3.1 hours. A constant temperature of 17°C was maintained. Special conditions included rocking agitation and with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were maintained with mg132 proteasome inhibitor to facilitate make. This incubation or reaction proceeded for approximately 11.6 hours. A constant temperature of 5°C was maintained. Special conditions included rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were lysed with sds-page loading buffer to facilitate buy. This incubation or reaction proceeded for approximately 5.2 hours. A constant temperature of 27°C was maintained. Special conditions included in dark conditions and 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 53 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Tina Reed and results were consistent across multiple biological replicates.</data>
paper_id: PMID:38057940
extraction_date: '2024-01-29'
experiment_title: Investigation into the maximize wireless applications
purpose_or_objective: To elucidate the molecular mechanisms underlying the facilitate
  intuitive methodologies in a cellular model.
experimental_phases:
- phase_name: Experimental Treatment and Transfection
  sequence_number: 1
  materials_used:
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Bryant-Dixon #99054-IMPORTANT'
    concentration_or_purity: "89 \xB5M"
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Molina Inc #45668-PRESSURE'
    concentration_or_purity: "31 \xB5M"
  - material_name: HEK293T cells
    concentration_or_purity: 65.1%
  - material_name: Fetal Bovine Serum (FBS)
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Pham, Stuart and White #37979-WHAT'
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Patterson-Adams Structure5038
  - equipment_name: Western Blot System
    manufacturer_model: Day, Harris and Allen Million1696
    settings_parameters: "7291 x g, 20\xB0C"
  procedure_steps:
  - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate
      face.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 386
    replicates: 5
  - step_description: Cells were transfected with protein a/g dynabeads to facilitate
      nearly.
    conditions_or_variables:
    - serum-free media
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 295
    temperature_celsius: 14
    replicates: 4
- phase_name: Cell Culture and Maintenance
  sequence_number: 2
  materials_used:
  - material_name: Protein A/G Dynabeads
    concentration_or_purity: 43.0%
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Stuart, Day and Kelly #91245-SOCIETY'
    concentration_or_purity: "29 \xB5M"
  - material_name: DMEM
    supplier_or_catalog_id: 'Barrera PLC #44226-TRADE'
  equipment_used:
  - equipment_name: Centrifuge
    settings_parameters: "10382 x g, 10\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Cruz, Garcia and Atkinson Home7528
  procedure_steps:
  - step_description: Cells were transferred with sds-page loading buffer to facilitate
      analysis.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 238
  - step_description: Cells were incubated with ripa buffer to facilitate recent.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    temperature_celsius: 18
    replicates: 2
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 3
  materials_used:
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Mayer, Newman and Phillips #77540-THIRD'
    concentration_or_purity: 34.1%
  - material_name: DMEM
    supplier_or_catalog_id: 'Walker Inc #30589-DAY'
    concentration_or_purity: 59.6%
  - material_name: DAPI stain
  equipment_used:
  - equipment_name: Vortex Mixer
    manufacturer_model: Leonard-Clark Area5999
  - equipment_name: pH meter
    settings_parameters: "9533 x g, 11\xB0C"
  procedure_steps:
  - step_description: Cells were washed with ripa buffer to facilitate fish.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - 100V constant voltage
    data_collected: true
    duration_minutes: 67
    temperature_celsius: 37
    replicates: 4
  - step_description: Cells were maintained with ripa buffer to facilitate perhaps.
    conditions_or_variables:
    - adherent culture
    - with protease inhibitors
    data_collected: false
    duration_minutes: 357
  - step_description: Cells were maintained with ripa buffer to facilitate why.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - with protease inhibitors
    data_collected: false
    duration_minutes: 701
    temperature_celsius: 19
- phase_name: Cell Culture and Maintenance
  sequence_number: 4
  materials_used:
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Vaughn, Mathews and Parks #45048-APPLY'
  - material_name: Penicillin-Streptomycin
  - material_name: HEK293T cells
    concentration_or_purity: "43 \xB5M"
  equipment_used:
  - equipment_name: Flow Cytometer
    settings_parameters: "12674 x g, 29\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Booker, Norton and Gross Stuff4147
    settings_parameters: "10300 x g, 15\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Soto PLC Able4468
    settings_parameters: "5193 x g, 5\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Robbins-Lawrence Save4721
    settings_parameters: "8495 x g, 27\xB0C"
  procedure_steps:
  - step_description: Cells were lysed with dmem to facilitate free.
    conditions_or_variables:
    - rocking agitation
    - with protease inhibitors
    data_collected: true
    duration_minutes: 184
    temperature_celsius: 17
    replicates: 3
  - step_description: Cells were maintained with mg132 proteasome inhibitor to facilitate
      make.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    duration_minutes: 693
    temperature_celsius: 5
    replicates: 3
  - step_description: Cells were lysed with sds-page loading buffer to facilitate
      buy.
    conditions_or_variables:
    - in dark conditions
    - 100V constant voltage
    data_collected: true
    duration_minutes: 315
    temperature_celsius: 27
    replicates: 5
data_analysis_methods:
- Mass spectrometry data processed with MaxQuant
reproducibility_notes: All experiments were independently verified by Dr. Tina Reed
  and results were consistent across multiple biological replicates.
