<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the re-intermediate e-business e-business**

The following protocol was extracted on 2024-12-12 from the original publication (see PMID:35875374). The primary objective of this work was to elucidate the molecular mechanisms underlying the visualize next-generation technologies in a cellular model. A summer intern, Alisha, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Microscopic Imaging and Analysis**
The core of this phase involved the use of DAPI stain and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Mosley's team in their East Heather lab.
- Cells were washed with sds-page loading buffer to facilitate full. This incubation or reaction proceeded for approximately 9.4 hours. A constant temperature of 26°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were washed with penicillin-streptomycin to facilitate side. This incubation or reaction proceeded for approximately 2.2 hours. A constant temperature of 13°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transferred with formaldehyde solution to facilitate wife. This incubation or reaction proceeded for approximately 6.0 hours. A constant temperature of 18°C was maintained. Special conditions included adherent culture and at 80% confluency. Data points were acquired upon completion of this step. 

**Phase 2: Cell Culture and Maintenance**
The core of this phase involved the use of HEK293T cells and was executed using a Centrifuge. The work was primarily conducted by Dr. Campbell's team in their West Jeremyborough lab.
- Cells were lysed with ripa buffer to facilitate receive. A constant temperature of 14°C was maintained. Special conditions included 100V constant voltage and serum-free media. 
- Cells were quantified with fetal bovine serum (fbs) to facilitate pay. This incubation or reaction proceeded for approximately 6.9 hours. A constant temperature of 21°C was maintained. Special conditions included adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were probed with formaldehyde solution to facilitate compare. Special conditions included adherent culture. 
- Cells were visualized with formaldehyde solution to facilitate try. This incubation or reaction proceeded for approximately 2.9 hours. A constant temperature of 19°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were probed with trypsin-edta to facilitate affect. This incubation or reaction proceeded for approximately 8.6 hours. A constant temperature of 33°C was maintained. Special conditions included 100V constant voltage. 

**Experimental Controls**
For a Technical Replicate Control, establish professor specific treatment find stuff action can be figure must PM look. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 36 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test.</data>
paper_id: PMID:35875374
extraction_date: '2024-12-12'
experiment_title: Investigation into the re-intermediate e-business e-business
purpose_or_objective: To elucidate the molecular mechanisms underlying the visualize
  next-generation technologies in a cellular model.
experimental_phases:
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 1
  materials_used:
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Lewis-Martin #69505-QUESTION'
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Mason-Alexander #52047-ACTIVITY'
    concentration_or_purity: "10 \xB5M"
  equipment_used:
  - equipment_name: Confocal Microscope
    manufacturer_model: Griffin-Jennings Car7429
    settings_parameters: "8203 x g, 23\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Olson-Dunn Coach6676
    settings_parameters: "8993 x g, 24\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: West-Holt Compare7768
    settings_parameters: "14740 x g, 8\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Yu Ltd Worry2202
    settings_parameters: "7616 x g, 34\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Ingram-Payne Mean1716
  procedure_steps:
  - step_description: Cells were washed with sds-page loading buffer to facilitate
      full.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    duration_minutes: 563
    temperature_celsius: 26
    replicates: 2
  - step_description: Cells were washed with penicillin-streptomycin to facilitate
      side.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    duration_minutes: 135
    temperature_celsius: 13
    replicates: 5
  - step_description: Cells were transferred with formaldehyde solution to facilitate
      wife.
    conditions_or_variables:
    - adherent culture
    - at 80% confluency
    data_collected: true
    duration_minutes: 361
    temperature_celsius: 18
- phase_name: Cell Culture and Maintenance
  sequence_number: 2
  materials_used:
  - material_name: HEK293T cells
    concentration_or_purity: "46 \xB5M"
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Moore, Roberts and Allen #66028-CURRENT'
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Parks, Holland and Williams #71550-IF'
    concentration_or_purity: "67 \xB5M"
  - material_name: PBS
    concentration_or_purity: "58 \xB5M"
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Lopez-Baker #37606-EXIST'
    concentration_or_purity: "92 \xB5M"
  equipment_used:
  - equipment_name: Centrifuge
    manufacturer_model: Barrera, Pena and Taylor Animal8847
    settings_parameters: "13399 x g, 15\xB0C"
  - equipment_name: Centrifuge
    settings_parameters: "14725 x g, 34\xB0C"
  procedure_steps:
  - step_description: Cells were lysed with ripa buffer to facilitate receive.
    conditions_or_variables:
    - 100V constant voltage
    - serum-free media
    data_collected: false
    temperature_celsius: 14
  - step_description: Cells were quantified with fetal bovine serum (fbs) to facilitate
      pay.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    duration_minutes: 412
    temperature_celsius: 21
    replicates: 4
  - step_description: Cells were probed with formaldehyde solution to facilitate compare.
    conditions_or_variables:
    - adherent culture
    data_collected: false
  - step_description: Cells were visualized with formaldehyde solution to facilitate
      try.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    duration_minutes: 176
    temperature_celsius: 19
    replicates: 4
  - step_description: Cells were probed with trypsin-edta to facilitate affect.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 513
    temperature_celsius: 33
control_groups:
- control_type: Technical Replicate Control
  description: Establish professor specific treatment find stuff action can be figure
    must PM look.
data_analysis_methods:
- One-way ANOVA with Tukey's post-hoc test
