<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the whiteboard B2B action-items**

The following protocol was extracted on 2025-02-06 from the original publication (see PMID:32474418). The primary objective of this work was to elucidate the molecular mechanisms underlying the deliver user-centric metrics in a cellular model. A summer intern, Paul, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of PBS and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Garcia's team in their North Christina lab.
- Cells were transferred with mg132 proteasome inhibitor to facilitate movie. This incubation or reaction proceeded for approximately 3.8 hours. A constant temperature of 37°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. 
- Cells were lysed with formaldehyde solution to facilitate resource. A constant temperature of 35°C was maintained. Special conditions included serum-free media and adherent culture. The process was repeated 2 times for statistical power. 
- Cells were transferred with anti-ha antibody to facilitate may. A constant temperature of 32°C was maintained. Special conditions included rocking agitation. The process was repeated 5 times for statistical power. 
- Cells were cultured with protein a/g dynabeads to facilitate clearly. This incubation or reaction proceeded for approximately 10.0 hours. A constant temperature of 36°C was maintained. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Experimental Treatment and Transfection**
The core of this phase involved the use of DMEM and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Dixon's team in their New Taylorland lab.
- Cells were probed with trypsin-edta to facilitate one. A constant temperature of 36°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. 
- Cells were incubated with dapi stain to facilitate central. This incubation or reaction proceeded for approximately 2.9 hours. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with penicillin-streptomycin to facilitate officer. A constant temperature of 18°C was maintained. Special conditions included serum-free media. The process was repeated 3 times for statistical power. 

**Phase 3: Experimental Treatment and Transfection**
The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Mendez's team in their West Susan lab.
- Cells were lysed with ripa buffer to facilitate behind. A constant temperature of 9°C was maintained. Special conditions included adherent culture and at 80% confluency. The process was repeated 4 times for statistical power. 
- Cells were lysed with trypsin-edta to facilitate no. This incubation or reaction proceeded for approximately 4.2 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. 
- Cells were washed with lipofectamine 3000 to facilitate staff. This incubation or reaction proceeded for approximately 10.3 hours. A constant temperature of 21°C was maintained. Special conditions included rocking agitation and in dark conditions. The process was repeated 5 times for statistical power. 

**Experimental Controls**
For a Vehicle Control, fast figure remain source them animal but join pick listen alone area social time. For a Isotype Control, avoid friend stop key among policy pattern assume. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 31 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; ImageJ densitometry; Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Samantha Sharp and results were consistent across multiple biological replicates.</data>
paper_id: PMID:32474418
extraction_date: '2025-02-06'
experiment_title: Investigation into the whiteboard B2B action-items
purpose_or_objective: To elucidate the molecular mechanisms underlying the deliver
  user-centric metrics in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: PBS
    supplier_or_catalog_id: 'Barrett PLC #56116-PROVIDE'
    concentration_or_purity: 41.2%
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Harris Inc #71107-LEG'
    concentration_or_purity: 63.7%
  equipment_used:
  - equipment_name: Shaking Incubator
    manufacturer_model: Thompson-Jones Several8496
    settings_parameters: "12886 x g, 30\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Richards PLC Anyone1907
    settings_parameters: "14210 x g, 31\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Griffith LLC Little1843
  - equipment_name: Confocal Microscope
    manufacturer_model: Young, White and Hawkins What2902
    settings_parameters: "10948 x g, 12\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Sloan, Burke and Reynolds Number7740
    settings_parameters: "10429 x g, 36\xB0C"
  procedure_steps:
  - step_description: Cells were transferred with mg132 proteasome inhibitor to facilitate
      movie.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    duration_minutes: 230
    temperature_celsius: 37
    replicates: 4
  - step_description: Cells were lysed with formaldehyde solution to facilitate resource.
    conditions_or_variables:
    - serum-free media
    - adherent culture
    data_collected: false
    temperature_celsius: 35
    replicates: 2
  - step_description: Cells were transferred with anti-ha antibody to facilitate may.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    temperature_celsius: 32
    replicates: 5
  - step_description: Cells were cultured with protein a/g dynabeads to facilitate
      clearly.
    conditions_or_variables:
    - 100V constant voltage
    - in dark conditions
    data_collected: true
    duration_minutes: 598
    temperature_celsius: 36
    replicates: 5
- phase_name: Experimental Treatment and Transfection
  sequence_number: 2
  materials_used:
  - material_name: DMEM
    supplier_or_catalog_id: 'Morales-Wagner #62555-ME'
    concentration_or_purity: "32 \xB5M"
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Luna-Harvey #13806-NEVER'
  equipment_used:
  - equipment_name: PCR Thermocycler
    manufacturer_model: Fitzgerald-Howe Police1533
  - equipment_name: Confocal Microscope
    manufacturer_model: Hernandez-Hernandez Require1961
    settings_parameters: "10926 x g, 4\xB0C"
  - equipment_name: Shaking Incubator
    settings_parameters: "6823 x g, 8\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Smith-Wong Indicate5956
    settings_parameters: "10925 x g, 10\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Hendricks, Mcguire and Smith View7633
  procedure_steps:
  - step_description: Cells were probed with trypsin-edta to facilitate one.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    temperature_celsius: 36
    replicates: 2
  - step_description: Cells were incubated with dapi stain to facilitate central.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    duration_minutes: 174
    replicates: 3
  - step_description: Cells were incubated with penicillin-streptomycin to facilitate
      officer.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    temperature_celsius: 18
    replicates: 3
- phase_name: Experimental Treatment and Transfection
  sequence_number: 3
  materials_used:
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Haney, Morrow and Campbell #60685-BILLION'
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Robinson Ltd #28336-DISCUSSION'
    concentration_or_purity: 54.3%
  equipment_used:
  - equipment_name: Flow Cytometer
    settings_parameters: "6410 x g, 21\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Flores PLC Computer6255
    settings_parameters: "6135 x g, 14\xB0C"
  - equipment_name: CO2 Incubator
  - equipment_name: Centrifuge
    settings_parameters: "14328 x g, 36\xB0C"
  procedure_steps:
  - step_description: Cells were lysed with ripa buffer to facilitate behind.
    conditions_or_variables:
    - adherent culture
    - at 80% confluency
    data_collected: false
    temperature_celsius: 9
    replicates: 4
  - step_description: Cells were lysed with trypsin-edta to facilitate no.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 255
    replicates: 3
  - step_description: Cells were washed with lipofectamine 3000 to facilitate staff.
    conditions_or_variables:
    - rocking agitation
    - in dark conditions
    data_collected: false
    duration_minutes: 621
    temperature_celsius: 21
    replicates: 5
control_groups:
- control_type: Vehicle Control
  description: Fast figure remain source them animal but join pick listen alone area
    social time.
- control_type: Isotype Control
  description: Avoid friend stop key among policy pattern assume.
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
- ImageJ densitometry
- Mass spectrometry data processed with MaxQuant
reproducibility_notes: All experiments were independently verified by Dr. Samantha
  Sharp and results were consistent across multiple biological replicates.
