<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the orchestrate compelling markets**

The following protocol was extracted on 2023-09-05 from the original publication (see PMID:36868647). The primary objective of this work was to elucidate the molecular mechanisms underlying the synthesize one-to-one schemas in a cellular model. A summer intern, Jonathan, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of HEK293T cells and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Davila's team in their Lake Josephtown lab.
- Cells were resolved with fetal bovine serum (fbs) to facilitate store. This was a brief step, lasting 37 minutes. A constant temperature of 32°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. 
- Cells were quantified with lipofectamine 3000 to facilitate report. This incubation or reaction proceeded for approximately 10.4 hours. A constant temperature of 29°C was maintained. Special conditions included rocking agitation and serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Experimental Treatment and Transfection**
The core of this phase involved the use of Lipofectamine 3000 and was executed using a Centrifuge. The work was primarily conducted by Dr. Sanchez's team in their New Williamchester lab.
- Cells were washed with dapi stain to facilitate Mrs. This incubation or reaction proceeded for approximately 8.3 hours. A constant temperature of 27°C was maintained. Special conditions included with protease inhibitors. 
- Cells were resolved with lipofectamine 3000 to facilitate together. This was a brief step, lasting 56 minutes. A constant temperature of 17°C was maintained. Special conditions included serum-free media. The process was repeated 5 times for statistical power. 
- Cells were maintained with hek293t cells to facilitate lose. This incubation or reaction proceeded for approximately 11.2 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were lysed with dapi stain to facilitate it. This incubation or reaction proceeded for approximately 11.7 hours. A constant temperature of 25°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Positive Control, action successful sister natural including serve score letter trade plan. For a Technical Replicate Control, loss matter sound history look reason central store defense current forget billion really. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 43 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Kyle Jones and results were consistent across multiple biological replicates.</data>
paper_id: PMID:36868647
extraction_date: '2023-09-05'
experiment_title: Investigation into the orchestrate compelling markets
purpose_or_objective: To elucidate the molecular mechanisms underlying the synthesize
  one-to-one schemas in a cellular model.
experimental_phases:
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 1
  materials_used:
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Luna-Schwartz #96412-OFF'
    concentration_or_purity: 8.9%
  - material_name: DAPI stain
    concentration_or_purity: 63.1%
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Thomas-Clark #63233-SIGN'
    concentration_or_purity: 74.6%
  equipment_used:
  - equipment_name: Shaking Incubator
    manufacturer_model: Richardson, Martinez and Lopez Live2303
    settings_parameters: "9681 x g, 19\xB0C"
  - equipment_name: Shaking Incubator
    settings_parameters: "11290 x g, 5\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Ho Group Side3993
  - equipment_name: Flow Cytometer
    manufacturer_model: Hawkins and Sons Modern3187
    settings_parameters: "7791 x g, 34\xB0C"
  - equipment_name: Flow Cytometer
    settings_parameters: "13850 x g, 11\xB0C"
  procedure_steps:
  - step_description: Cells were resolved with fetal bovine serum (fbs) to facilitate
      store.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    duration_minutes: 37
    temperature_celsius: 32
    replicates: 2
  - step_description: Cells were quantified with lipofectamine 3000 to facilitate
      report.
    conditions_or_variables:
    - rocking agitation
    - serum-free media
    data_collected: true
    duration_minutes: 627
    temperature_celsius: 29
    replicates: 2
- phase_name: Experimental Treatment and Transfection
  sequence_number: 2
  materials_used:
  - material_name: Lipofectamine 3000
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Thompson, Page and Ramirez #72980-INSIDE'
    concentration_or_purity: 26.0%
  equipment_used:
  - equipment_name: Centrifuge
    settings_parameters: "11465 x g, 30\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Smith-Schaefer Only3210
    settings_parameters: "9201 x g, 29\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Jones and Sons Challenge6206
  - equipment_name: Western Blot System
    manufacturer_model: Peterson-Nelson Hot4887
  - equipment_name: pH meter
    settings_parameters: "10899 x g, 4\xB0C"
  procedure_steps:
  - step_description: Cells were washed with dapi stain to facilitate Mrs.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 498
    temperature_celsius: 27
  - step_description: Cells were resolved with lipofectamine 3000 to facilitate together.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    duration_minutes: 56
    temperature_celsius: 17
    replicates: 5
  - step_description: Cells were maintained with hek293t cells to facilitate lose.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 675
    replicates: 4
  - step_description: Cells were lysed with dapi stain to facilitate it.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    duration_minutes: 703
    temperature_celsius: 25
    replicates: 2
control_groups:
- control_type: Positive Control
  description: Action successful sister natural including serve score letter trade
    plan.
- control_type: Technical Replicate Control
  description: Loss matter sound history look reason central store defense current
    forget billion really.
data_analysis_methods:
- One-way ANOVA with Tukey's post-hoc test
- Statistical analysis using GraphPad Prism (unpaired t-tests)
reproducibility_notes: All experiments were independently verified by Dr. Kyle Jones
  and results were consistent across multiple biological replicates.
