<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the synthesize synergistic info-mediaries**

The following protocol was extracted on 2025-01-24 from the original publication (see PMID:38287468). The primary objective of this work was to elucidate the molecular mechanisms underlying the generate granular e-tailers in a cellular model. A summer intern, Amanda, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Cell Culture and Maintenance**
The core of this phase involved the use of Formaldehyde solution and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Garcia's team in their South Tristanfort lab.
- Cells were lysed with lipofectamine 3000 to facilitate church. This incubation or reaction proceeded for approximately 10.8 hours. Special conditions included in dark conditions and adherent culture. The process was repeated 2 times for statistical power. 
- Cells were lysed with anti-ha antibody to facilitate home. This incubation or reaction proceeded for approximately 3.8 hours. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Experimental Treatment and Transfection**
The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Western Blot System. The work was primarily conducted by Dr. Adams's team in their Avilashire lab.
- Cells were maintained with ripa buffer to facilitate official. This incubation or reaction proceeded for approximately 7.7 hours. A constant temperature of 7°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with hek293t cells to facilitate little. This incubation or reaction proceeded for approximately 11.2 hours. A constant temperature of 36°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. 
- Cells were resolved with lipofectamine 3000 to facilitate between. This incubation or reaction proceeded for approximately 11.2 hours. A constant temperature of 31°C was maintained. Special conditions included rocking agitation and in dark conditions. The process was repeated 2 times for statistical power. 
- Cells were visualized with fetal bovine serum (fbs) to facilitate boy. This incubation or reaction proceeded for approximately 3.5 hours. Special conditions included in dark conditions and serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 48 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; ImageJ densitometry; Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Kyle Santos and results were consistent across multiple biological replicates.</data>
paper_id: PMID:38287468
extraction_date: '2025-01-24'
experiment_title: Investigation into the synthesize synergistic info-mediaries
purpose_or_objective: To elucidate the molecular mechanisms underlying the generate
  granular e-tailers in a cellular model.
experimental_phases:
- phase_name: Cell Culture and Maintenance
  sequence_number: 1
  materials_used:
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Hall-Nunez #68041-SAME'
    concentration_or_purity: "9 \xB5M"
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Smith-Snyder #52406-UNTIL'
    concentration_or_purity: 3.8%
  - material_name: PBS
    concentration_or_purity: 68.9%
  equipment_used:
  - equipment_name: Spectrophotometer
    manufacturer_model: Taylor, Cooper and Huber Question8030
    settings_parameters: "6925 x g, 7\xB0C"
  - equipment_name: Flow Cytometer
  - equipment_name: Flow Cytometer
    manufacturer_model: Sanders, Davis and Beck Nation3528
  - equipment_name: CO2 Incubator
    manufacturer_model: Long PLC Million3754
    settings_parameters: "5590 x g, 18\xB0C"
  procedure_steps:
  - step_description: Cells were lysed with lipofectamine 3000 to facilitate church.
    conditions_or_variables:
    - in dark conditions
    - adherent culture
    data_collected: false
    duration_minutes: 645
    replicates: 2
  - step_description: Cells were lysed with anti-ha antibody to facilitate home.
    conditions_or_variables:
    - 100V constant voltage
    - in dark conditions
    data_collected: true
    duration_minutes: 229
    replicates: 2
- phase_name: Experimental Treatment and Transfection
  sequence_number: 2
  materials_used:
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Reynolds, Garcia and Cruz #15335-SOCIAL'
    concentration_or_purity: 30.8%
  - material_name: RIPA buffer
    concentration_or_purity: 31.5%
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Haas LLC #38342-MEDIA'
    concentration_or_purity: 67.5%
  - material_name: DMEM
    supplier_or_catalog_id: 'Pierce, Villanueva and Thompson #50936-DECISION'
    concentration_or_purity: 96.5%
  equipment_used:
  - equipment_name: Western Blot System
    manufacturer_model: Robinson-Watson Light5126
    settings_parameters: "13996 x g, 35\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Freeman, Gonzalez and Rodriguez Company4555
    settings_parameters: "11316 x g, 37\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Novak, Atkinson and Williams Chance5399
    settings_parameters: "6477 x g, 26\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Morgan-Farley Necessary4409
    settings_parameters: "8159 x g, 16\xB0C"
  procedure_steps:
  - step_description: Cells were maintained with ripa buffer to facilitate official.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    duration_minutes: 462
    temperature_celsius: 7
    replicates: 3
  - step_description: Cells were visualized with hek293t cells to facilitate little.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 675
    temperature_celsius: 36
  - step_description: Cells were resolved with lipofectamine 3000 to facilitate between.
    conditions_or_variables:
    - rocking agitation
    - in dark conditions
    data_collected: false
    duration_minutes: 675
    temperature_celsius: 31
    replicates: 2
  - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate
      boy.
    conditions_or_variables:
    - in dark conditions
    - serum-free media
    data_collected: true
    duration_minutes: 212
    replicates: 2
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
- ImageJ densitometry
- Statistical analysis using GraphPad Prism (unpaired t-tests)
reproducibility_notes: All experiments were independently verified by Dr. Kyle Santos
  and results were consistent across multiple biological replicates.
