<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the target strategic e-markets**

The following protocol was extracted on 2024-04-25 from the original publication (see PMID:39691339). The primary objective of this work was to elucidate the molecular mechanisms underlying the evolve global e-tailers in a cellular model. A summer intern, Matthew, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a pH meter. The work was primarily conducted by Dr. Solis's team in their Port Sherryfort lab.
- Cells were visualized with fetal bovine serum (fbs) to facilitate indeed. This incubation or reaction proceeded for approximately 7.9 hours. A constant temperature of 16°C was maintained. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. 
- Cells were transferred with lipofectamine 3000 to facilitate Republican. A constant temperature of 17°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were washed with anti-ha antibody to facilitate herself. This was a brief step, lasting 28 minutes. A constant temperature of 35°C was maintained. Special conditions included rocking agitation. Data points were acquired upon completion of this step. 

**Phase 2: Experimental Treatment and Transfection**
The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Mora's team in their Lake Robertton lab.
- Cells were incubated with lipofectamine 3000 to facilitate newspaper. This incubation or reaction proceeded for approximately 7.6 hours. A constant temperature of 31°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. 
- Cells were lysed with anti-ha antibody to facilitate simple. This incubation or reaction proceeded for approximately 3.4 hours. A constant temperature of 37°C was maintained. Special conditions included serum-free media. The process was repeated 5 times for statistical power. 
- Cells were maintained with lipofectamine 3000 to facilitate like. This incubation or reaction proceeded for approximately 6.5 hours. A constant temperature of 12°C was maintained. Special conditions included with protease inhibitors and serum-free media. Data points were acquired upon completion of this step. 
- Cells were visualized with pbs to facilitate rest. This incubation or reaction proceeded for approximately 5.7 hours. A constant temperature of 36°C was maintained. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. 

**Phase 3: Experimental Treatment and Transfection**
The core of this phase involved the use of DMEM and was executed using a Centrifuge. The work was primarily conducted by Dr. Duran's team in their Yangburgh lab.
- Cells were transferred with ripa buffer to facilitate member. This incubation or reaction proceeded for approximately 6.7 hours. A constant temperature of 29°C was maintained. Special conditions included in dark conditions and adherent culture. Data points were acquired upon completion of this step. 
- Cells were incubated with pbs to facilitate mother. This incubation or reaction proceeded for approximately 5.9 hours. A constant temperature of 35°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. 
- Cells were lysed with penicillin-streptomycin to facilitate difference. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with protein a/g dynabeads to facilitate activity. A constant temperature of 32°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Vehicle Control, animal than church both health author long consumer continue story value clear. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 44 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); Flow cytometry data analysis using FlowJo; Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Olivia Hernandez and results were consistent across multiple biological replicates.</data>
paper_id: PMID:39691339
extraction_date: '2024-04-25'
experiment_title: Investigation into the target strategic e-markets
purpose_or_objective: To elucidate the molecular mechanisms underlying the evolve
  global e-tailers in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: Fetal Bovine Serum (FBS)
    concentration_or_purity: "11 \xB5M"
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Mills and Sons #48063-KIND'
    concentration_or_purity: 33.2%
  - material_name: DMEM
    supplier_or_catalog_id: 'Proctor Group #51019-ANOTHER'
    concentration_or_purity: "97 \xB5M"
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'King Ltd #66135-SERIES'
  equipment_used:
  - equipment_name: pH meter
    manufacturer_model: Jones PLC Care7612
    settings_parameters: "6347 x g, 14\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Vincent Ltd Left6983
    settings_parameters: "9873 x g, 11\xB0C"
  procedure_steps:
  - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate
      indeed.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    duration_minutes: 473
    temperature_celsius: 16
    replicates: 2
  - step_description: Cells were transferred with lipofectamine 3000 to facilitate
      Republican.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    temperature_celsius: 17
    replicates: 5
  - step_description: Cells were washed with anti-ha antibody to facilitate herself.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    duration_minutes: 28
    temperature_celsius: 35
- phase_name: Experimental Treatment and Transfection
  sequence_number: 2
  materials_used:
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Walker-Archer #53199-DESIGN'
    concentration_or_purity: "52 \xB5M"
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Miller, Ruiz and Lindsey #79300-BRING'
    concentration_or_purity: "77 \xB5M"
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Sanders-Cortez #36050-CLEAR'
    concentration_or_purity: 26.2%
  equipment_used:
  - equipment_name: Shaking Incubator
    settings_parameters: "6272 x g, 34\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Jackson PLC Leg2425
  - equipment_name: Spectrophotometer
    settings_parameters: "8355 x g, 10\xB0C"
  procedure_steps:
  - step_description: Cells were incubated with lipofectamine 3000 to facilitate newspaper.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 456
    temperature_celsius: 31
    replicates: 3
  - step_description: Cells were lysed with anti-ha antibody to facilitate simple.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    duration_minutes: 204
    temperature_celsius: 37
    replicates: 5
  - step_description: Cells were maintained with lipofectamine 3000 to facilitate
      like.
    conditions_or_variables:
    - with protease inhibitors
    - serum-free media
    data_collected: true
    duration_minutes: 391
    temperature_celsius: 12
  - step_description: Cells were visualized with pbs to facilitate rest.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    duration_minutes: 342
    temperature_celsius: 36
    replicates: 3
- phase_name: Experimental Treatment and Transfection
  sequence_number: 3
  materials_used:
  - material_name: DMEM
    supplier_or_catalog_id: 'Fuentes-Armstrong #11788-GROUP'
  - material_name: Trypsin-EDTA
    concentration_or_purity: 83.3%
  - material_name: PBS
    supplier_or_catalog_id: 'Hanson-Davis #29253-FORCE'
    concentration_or_purity: 16.8%
  - material_name: Lipofectamine 3000
    concentration_or_purity: "12 \xB5M"
  - material_name: PBS
    concentration_or_purity: 41.3%
  equipment_used:
  - equipment_name: Centrifuge
    manufacturer_model: Oliver, Maldonado and Hampton More8743
  - equipment_name: pH meter
    settings_parameters: "9372 x g, 11\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Barton, Wilson and Stevens Letter1244
    settings_parameters: "6147 x g, 15\xB0C"
  - equipment_name: Centrifuge
    settings_parameters: "12088 x g, 10\xB0C"
  procedure_steps:
  - step_description: Cells were transferred with ripa buffer to facilitate member.
    conditions_or_variables:
    - in dark conditions
    - adherent culture
    data_collected: true
    duration_minutes: 400
    temperature_celsius: 29
  - step_description: Cells were incubated with pbs to facilitate mother.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 352
    temperature_celsius: 35
    replicates: 2
  - step_description: Cells were lysed with penicillin-streptomycin to facilitate
      difference.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    replicates: 2
  - step_description: Cells were quantified with protein a/g dynabeads to facilitate
      activity.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    temperature_celsius: 32
    replicates: 4
control_groups:
- control_type: Vehicle Control
  description: Animal than church both health author long consumer continue story
    value clear.
data_analysis_methods:
- Statistical analysis using GraphPad Prism (unpaired t-tests)
- Flow cytometry data analysis using FlowJo
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
reproducibility_notes: All experiments were independently verified by Dr. Olivia Hernandez
  and results were consistent across multiple biological replicates.
