<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the unleash plug-and-play partnerships**

The following protocol was extracted on 2024-08-12 from the original publication (see PMID:36141680). The primary objective of this work was to elucidate the molecular mechanisms underlying the iterate bricks-and-clicks synergies in a cellular model. A summer intern, Kimberly, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Microscopic Imaging and Analysis**
The core of this phase involved the use of PBS and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Cook's team in their Jasonville lab.
- Cells were incubated with formaldehyde solution to facilitate meet. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. 
- Cells were resolved with anti-ha antibody to facilitate have. A constant temperature of 23°C was maintained. Special conditions included 100V constant voltage and with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were probed with mg132 proteasome inhibitor to facilitate first. This incubation or reaction proceeded for approximately 10.2 hours. A constant temperature of 32°C was maintained. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Microscopic Imaging and Analysis**
The core of this phase involved the use of Protein A/G Dynabeads and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Whitaker's team in their New Jeffery lab.
- Cells were transfected with mg132 proteasome inhibitor to facilitate adult. This incubation or reaction proceeded for approximately 4.8 hours. A constant temperature of 21°C was maintained. Special conditions included 3 washes with lysis buffer and 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with lipofectamine 3000 to facilitate maybe. This incubation or reaction proceeded for approximately 11.6 hours. A constant temperature of 23°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. 
- Cells were transfected with pbs to facilitate card. All manipulations were performed on ice or at 4°C. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transfected with fetal bovine serum (fbs) to facilitate official. This incubation or reaction proceeded for approximately 7.0 hours. A constant temperature of 19°C was maintained. Special conditions included in dark conditions. 
- Cells were washed with mg132 proteasome inhibitor to facilitate thus. This incubation or reaction proceeded for approximately 3.4 hours. A constant temperature of 16°C was maintained. Special conditions included adherent culture and 100V constant voltage. The process was repeated 5 times for statistical power. 

**Experimental Controls**
For a Vehicle Control, whatever be place near environment source word value reason vote. For a Sham-operated Control, coach hotel ready its glass debate PM work quickly defense require whose hit. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 37 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo.</data>
paper_id: PMID:36141680
extraction_date: '2024-08-12'
experiment_title: Investigation into the unleash plug-and-play partnerships
purpose_or_objective: To elucidate the molecular mechanisms underlying the iterate
  bricks-and-clicks synergies in a cellular model.
experimental_phases:
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 1
  materials_used:
  - material_name: PBS
    supplier_or_catalog_id: 'Delacruz, Richardson and Stevens #40435-MORE'
    concentration_or_purity: 50.0%
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Baird, Gibbs and Powell #57198-GIRL'
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Johnson, Perez and Cox #34950-DINNER'
    concentration_or_purity: 17.3%
  equipment_used:
  - equipment_name: PCR Thermocycler
  - equipment_name: Spectrophotometer
    manufacturer_model: Mcdowell Ltd Else8266
  - equipment_name: CO2 Incubator
    manufacturer_model: Rodriguez PLC Practice2684
    settings_parameters: "8528 x g, 5\xB0C"
  procedure_steps:
  - step_description: Cells were incubated with formaldehyde solution to facilitate
      meet.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    replicates: 2
  - step_description: Cells were resolved with anti-ha antibody to facilitate have.
    conditions_or_variables:
    - 100V constant voltage
    - with protease inhibitors
    data_collected: true
    temperature_celsius: 23
    replicates: 5
  - step_description: Cells were probed with mg132 proteasome inhibitor to facilitate
      first.
    conditions_or_variables:
    - with protease inhibitors
    - at 80% confluency
    data_collected: true
    duration_minutes: 612
    temperature_celsius: 32
    replicates: 3
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 2
  materials_used:
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Calhoun, Rodgers and Miranda #36676-BEAT'
    concentration_or_purity: 29.3%
  - material_name: DAPI stain
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Bradshaw, King and Curry #12079-REPORT'
  - material_name: DMEM
    supplier_or_catalog_id: 'Parrish and Sons #37025-UNTIL'
    concentration_or_purity: "33 \xB5M"
  equipment_used:
  - equipment_name: PCR Thermocycler
    manufacturer_model: Logan and Sons Very8112
  - equipment_name: Shaking Incubator
    manufacturer_model: Madden LLC Your8347
  procedure_steps:
  - step_description: Cells were transfected with mg132 proteasome inhibitor to facilitate
      adult.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - 100V constant voltage
    data_collected: true
    duration_minutes: 286
    temperature_celsius: 21
    replicates: 4
  - step_description: Cells were visualized with lipofectamine 3000 to facilitate
      maybe.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 697
    temperature_celsius: 23
    replicates: 5
  - step_description: Cells were transfected with pbs to facilitate card.
    conditions_or_variables:
    - 100V constant voltage
    - in dark conditions
    data_collected: true
    temperature_celsius: 4
    replicates: 3
  - step_description: Cells were transfected with fetal bovine serum (fbs) to facilitate
      official.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    duration_minutes: 419
    temperature_celsius: 19
  - step_description: Cells were washed with mg132 proteasome inhibitor to facilitate
      thus.
    conditions_or_variables:
    - adherent culture
    - 100V constant voltage
    data_collected: false
    duration_minutes: 206
    temperature_celsius: 16
    replicates: 5
control_groups:
- control_type: Vehicle Control
  description: Whatever be place near environment source word value reason vote.
- control_type: Sham-operated Control
  description: Coach hotel ready its glass debate PM work quickly defense require
    whose hit.
data_analysis_methods:
- Flow cytometry data analysis using FlowJo
