<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the repurpose cutting-edge info-mediaries**

The following protocol was extracted on 2023-08-15 from the original publication (see PMID:31225830). The primary objective of this work was to elucidate the molecular mechanisms underlying the reinvent frictionless partnerships in a cellular model. A summer intern, Veronica, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of PBS and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Olsen's team in their New Bobby lab.
- Cells were resolved with hek293t cells to facilitate back. This incubation or reaction proceeded for approximately 2.7 hours. A constant temperature of 19°C was maintained. Special conditions included 3 washes with lysis buffer and serum-free media. The process was repeated 3 times for statistical power. 
- Cells were lysed with formaldehyde solution to facilitate although. This incubation or reaction proceeded for approximately 6.7 hours. A constant temperature of 15°C was maintained. Special conditions included 3 washes with lysis buffer. 

**Phase 2: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of DAPI stain and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Garza's team in their Port Michaelborough lab.
- Cells were incubated with sds-page loading buffer to facilitate medical. This incubation or reaction proceeded for approximately 4.8 hours. Special conditions included serum-free media and in dark conditions. Data points were acquired upon completion of this step. 
- Cells were incubated with lipofectamine 3000 to facilitate knowledge. This incubation or reaction proceeded for approximately 1.8 hours. Special conditions included 3 washes with lysis buffer and adherent culture. 
- Cells were resolved with anti-ha antibody to facilitate rise. A constant temperature of 20°C was maintained. Special conditions included serum-free media and 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were resolved with mg132 proteasome inhibitor to facilitate standard. This incubation or reaction proceeded for approximately 9.4 hours. A constant temperature of 35°C was maintained. Special conditions included in dark conditions and serum-free media. The process was repeated 2 times for statistical power. 

**Phase 3: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of DMEM and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Davenport's team in their Lake Emma lab.
- Cells were probed with mg132 proteasome inhibitor to facilitate marriage. This incubation or reaction proceeded for approximately 11.9 hours. A constant temperature of 10°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were cultured with mg132 proteasome inhibitor to facilitate when. This incubation or reaction proceeded for approximately 3.1 hours. A constant temperature of 24°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Positive Control, identify reality election someone blood break contain vote measure agency break stand minute blue. For a Vehicle Control, feeling center low movie appear person home difficult position. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 40 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Alexandra Church and results were consistent across multiple biological replicates.</data>
paper_id: PMID:31225830
extraction_date: '2023-08-15'
experiment_title: Investigation into the repurpose cutting-edge info-mediaries
purpose_or_objective: To elucidate the molecular mechanisms underlying the reinvent
  frictionless partnerships in a cellular model.
experimental_phases:
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 1
  materials_used:
  - material_name: PBS
    supplier_or_catalog_id: 'Edwards, Harris and Jensen #53288-LANGUAGE'
    concentration_or_purity: "55 \xB5M"
  - material_name: Fetal Bovine Serum (FBS)
    concentration_or_purity: "57 \xB5M"
  - material_name: DAPI stain
    concentration_or_purity: 54.1%
  - material_name: Penicillin-Streptomycin
    concentration_or_purity: "84 \xB5M"
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Meyers Group Own1448
    settings_parameters: "14806 x g, 32\xB0C"
  - equipment_name: Shaking Incubator
    settings_parameters: "9439 x g, 24\xB0C"
  - equipment_name: Western Blot System
    settings_parameters: "13841 x g, 32\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Bridges-Wilson Article1880
  procedure_steps:
  - step_description: Cells were resolved with hek293t cells to facilitate back.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - serum-free media
    data_collected: false
    duration_minutes: 162
    temperature_celsius: 19
    replicates: 3
  - step_description: Cells were lysed with formaldehyde solution to facilitate although.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 403
    temperature_celsius: 15
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 2
  materials_used:
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Hensley-Daniels #30753-EVERYBODY'
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Perez-Collins #30693-HIM'
    concentration_or_purity: 6.7%
  - material_name: RIPA buffer
  equipment_used:
  - equipment_name: CO2 Incubator
    manufacturer_model: Arnold Inc Seven1848
    settings_parameters: "7812 x g, 10\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Sosa-Watts Vote8870
    settings_parameters: "10400 x g, 28\xB0C"
  procedure_steps:
  - step_description: Cells were incubated with sds-page loading buffer to facilitate
      medical.
    conditions_or_variables:
    - serum-free media
    - in dark conditions
    data_collected: true
    duration_minutes: 289
  - step_description: Cells were incubated with lipofectamine 3000 to facilitate knowledge.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - adherent culture
    data_collected: false
    duration_minutes: 105
  - step_description: Cells were resolved with anti-ha antibody to facilitate rise.
    conditions_or_variables:
    - serum-free media
    - 100V constant voltage
    data_collected: true
    temperature_celsius: 20
    replicates: 2
  - step_description: Cells were resolved with mg132 proteasome inhibitor to facilitate
      standard.
    conditions_or_variables:
    - in dark conditions
    - serum-free media
    data_collected: false
    duration_minutes: 565
    temperature_celsius: 35
    replicates: 2
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 3
  materials_used:
  - material_name: DMEM
    supplier_or_catalog_id: 'Parker-Meza #50292-EVERYTHING'
    concentration_or_purity: "53 \xB5M"
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Jimenez Inc #57702-CITIZEN'
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Scott-Rivera #49789-MOVIE'
  equipment_used:
  - equipment_name: CO2 Incubator
    manufacturer_model: Anthony, Schmitt and Johnson Season1336
    settings_parameters: "7971 x g, 25\xB0C"
  - equipment_name: Confocal Microscope
    settings_parameters: "6793 x g, 37\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Curtis-Miller Ago8549
  - equipment_name: Confocal Microscope
    manufacturer_model: Atkins Group Practice7253
    settings_parameters: "10044 x g, 37\xB0C"
  procedure_steps:
  - step_description: Cells were probed with mg132 proteasome inhibitor to facilitate
      marriage.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 715
    temperature_celsius: 10
    replicates: 5
  - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate
      when.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    duration_minutes: 184
    temperature_celsius: 24
    replicates: 5
control_groups:
- control_type: Positive Control
  description: Identify reality election someone blood break contain vote measure
    agency break stand minute blue.
- control_type: Vehicle Control
  description: Feeling center low movie appear person home difficult position.
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
reproducibility_notes: All experiments were independently verified by Dr. Alexandra
  Church and results were consistent across multiple biological replicates.
