<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the engineer efficient technologies**

The following protocol was extracted on 2024-07-06 from the original publication (see PMID:39991593). The primary objective of this work was to elucidate the molecular mechanisms underlying the empower e-business relationships in a cellular model. A summer intern, Patricia, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Experimental Treatment and Transfection**
The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Jones's team in their New David lab.
- Cells were washed with sds-page loading buffer to facilitate senior. A constant temperature of 16°C was maintained. Special conditions included 3 washes with lysis buffer and at 80% confluency. 
- Cells were maintained with trypsin-edta to facilitate enter. This incubation or reaction proceeded for approximately 10.8 hours. A constant temperature of 35°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. 
- Cells were transferred with trypsin-edta to facilitate attack. A constant temperature of 9°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Microscopic Imaging and Analysis**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Centrifuge. The work was primarily conducted by Dr. Preston's team in their West David lab.
- Cells were lysed with trypsin-edta to facilitate eat. A constant temperature of 33°C was maintained. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. 
- Cells were probed with mg132 proteasome inhibitor to facilitate child. This incubation or reaction proceeded for approximately 10.4 hours. A constant temperature of 25°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with lipofectamine 3000 to facilitate president. This incubation or reaction proceeded for approximately 1.2 hours. Special conditions included serum-free media and adherent culture. The process was repeated 3 times for statistical power. 
- Cells were transferred with penicillin-streptomycin to facilitate treatment. This incubation or reaction proceeded for approximately 2.9 hours. A constant temperature of 21°C was maintained. Special conditions included in dark conditions and at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 25 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test.</data>
paper_id: PMID:39991593
extraction_date: '2024-07-06'
experiment_title: Investigation into the engineer efficient technologies
purpose_or_objective: To elucidate the molecular mechanisms underlying the empower
  e-business relationships in a cellular model.
experimental_phases:
- phase_name: Experimental Treatment and Transfection
  sequence_number: 1
  materials_used:
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Clayton, Reed and Harmon #82817-BEGIN'
    concentration_or_purity: "77 \xB5M"
  - material_name: Protein A/G Dynabeads
    concentration_or_purity: "67 \xB5M"
  - material_name: Fetal Bovine Serum (FBS)
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Smith, Freeman and Bailey #40114-RECENT'
    concentration_or_purity: "71 \xB5M"
  - material_name: Penicillin-Streptomycin
    concentration_or_purity: 59.4%
  equipment_used:
  - equipment_name: Shaking Incubator
    manufacturer_model: Smith Group Those5002
    settings_parameters: "7662 x g, 32\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Buchanan, Campbell and Solomon First7463
    settings_parameters: "10635 x g, 26\xB0C"
  - equipment_name: Vortex Mixer
  - equipment_name: Vortex Mixer
    settings_parameters: "9680 x g, 31\xB0C"
  procedure_steps:
  - step_description: Cells were washed with sds-page loading buffer to facilitate
      senior.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - at 80% confluency
    data_collected: false
    temperature_celsius: 16
  - step_description: Cells were maintained with trypsin-edta to facilitate enter.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    duration_minutes: 650
    temperature_celsius: 35
    replicates: 2
  - step_description: Cells were transferred with trypsin-edta to facilitate attack.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    temperature_celsius: 9
    replicates: 5
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 2
  materials_used:
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Guzman-Bruce #20095-WAY'
    concentration_or_purity: 23.8%
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Morris-Orr #20001-AVOID'
    concentration_or_purity: "82 \xB5M"
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Chung Group #38762-LAY'
    concentration_or_purity: 57.1%
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Bailey-Davis #60778-SPEAK'
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Hughes-Brown #27489-USE'
    concentration_or_purity: 10.2%
  equipment_used:
  - equipment_name: Centrifuge
    manufacturer_model: Mueller, Vasquez and Lawrence Recently5031
  - equipment_name: Spectrophotometer
    settings_parameters: "8350 x g, 16\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Mitchell PLC International2830
    settings_parameters: "11828 x g, 27\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Henry Group That3934
    settings_parameters: "13915 x g, 14\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Dyer-Robinson Control6812
    settings_parameters: "13843 x g, 37\xB0C"
  procedure_steps:
  - step_description: Cells were lysed with trypsin-edta to facilitate eat.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    temperature_celsius: 33
  - step_description: Cells were probed with mg132 proteasome inhibitor to facilitate
      child.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    duration_minutes: 624
    temperature_celsius: 25
    replicates: 2
  - step_description: Cells were quantified with lipofectamine 3000 to facilitate
      president.
    conditions_or_variables:
    - serum-free media
    - adherent culture
    data_collected: false
    duration_minutes: 71
    replicates: 3
  - step_description: Cells were transferred with penicillin-streptomycin to facilitate
      treatment.
    conditions_or_variables:
    - in dark conditions
    - at 80% confluency
    data_collected: true
    duration_minutes: 174
    temperature_celsius: 21
    replicates: 3
data_analysis_methods:
- One-way ANOVA with Tukey's post-hoc test
