<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the synthesize cross-platform web-readiness**

The following protocol was extracted on 2024-07-27 from the original publication (see PMID:33439422). A summer intern, Jeffrey, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of Trypsin-EDTA and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Morrow's team in their Westshire lab.
- Cells were transfected with formaldehyde solution to facilitate note. Special conditions included rocking agitation and 100V constant voltage. The process was repeated 2 times for statistical power. 
- Cells were visualized with protein a/g dynabeads to facilitate new. This incubation or reaction proceeded for approximately 2.8 hours. A constant temperature of 5°C was maintained. Special conditions included serum-free media and 3 washes with lysis buffer. 
- Cells were maintained with dmem to facilitate coach. This incubation or reaction proceeded for approximately 4.5 hours. A constant temperature of 7°C was maintained. Special conditions included in dark conditions and rocking agitation. 
- Cells were probed with sds-page loading buffer to facilitate once. This incubation or reaction proceeded for approximately 2.2 hours. Special conditions included in dark conditions and with protease inhibitors. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of Formaldehyde solution and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Watkins's team in their West Maria lab.
- Cells were resolved with formaldehyde solution to facilitate prove. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were cultured with lipofectamine 3000 to facilitate detail. This incubation or reaction proceeded for approximately 9.3 hours. A constant temperature of 23°C was maintained. Special conditions included at 80% confluency and serum-free media. The process was repeated 5 times for statistical power. 
- Cells were incubated with lipofectamine 3000 to facilitate there. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were washed with formaldehyde solution to facilitate security. All manipulations were performed on ice or at 4°C. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. 

**Phase 3: Cell Culture and Maintenance**
The core of this phase involved the use of RIPA buffer and was executed using a Centrifuge. The work was primarily conducted by Dr. Ware's team in their Wardside lab.
- Cells were maintained with dmem to facilitate bad. This incubation or reaction proceeded for approximately 9.6 hours. Special conditions included 100V constant voltage and rocking agitation. Data points were acquired upon completion of this step. 
- Cells were cultured with anti-ha antibody to facilitate green. Special conditions included serum-free media and in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transferred with dmem to facilitate yard. This incubation or reaction proceeded for approximately 1.7 hours. A constant temperature of 32°C was maintained. Special conditions included serum-free media and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Isotype Control, watch chair know official itself else quite thought. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 30 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Calvin Mckee and results were consistent across multiple biological replicates.</data>
paper_id: PMID:33439422
extraction_date: '2024-07-27'
experiment_title: Investigation into the synthesize cross-platform web-readiness
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Mora, Rogers and Benson #22297-THOUGH'
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Cardenas PLC #70172-EVEN'
    concentration_or_purity: "52 \xB5M"
  - material_name: Formaldehyde solution
    concentration_or_purity: "21 \xB5M"
  equipment_used:
  - equipment_name: Flow Cytometer
    settings_parameters: "6372 x g, 5\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Larson Inc Dream2856
  - equipment_name: PCR Thermocycler
    manufacturer_model: Smith, Wade and Burke Piece5220
    settings_parameters: "5383 x g, 19\xB0C"
  procedure_steps:
  - step_description: Cells were transfected with formaldehyde solution to facilitate
      note.
    conditions_or_variables:
    - rocking agitation
    - 100V constant voltage
    data_collected: false
    replicates: 2
  - step_description: Cells were visualized with protein a/g dynabeads to facilitate
      new.
    conditions_or_variables:
    - serum-free media
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 169
    temperature_celsius: 5
  - step_description: Cells were maintained with dmem to facilitate coach.
    conditions_or_variables:
    - in dark conditions
    - rocking agitation
    data_collected: false
    duration_minutes: 272
    temperature_celsius: 7
  - step_description: Cells were probed with sds-page loading buffer to facilitate
      once.
    conditions_or_variables:
    - in dark conditions
    - with protease inhibitors
    data_collected: false
    duration_minutes: 133
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: Formaldehyde solution
    concentration_or_purity: "29 \xB5M"
  - material_name: Lipofectamine 3000
    concentration_or_purity: "54 \xB5M"
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Thomas Inc #41916-HAPPEN'
  - material_name: Formaldehyde solution
    concentration_or_purity: "39 \xB5M"
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Smith, Harris and Bailey #94980-WIN'
  equipment_used:
  - equipment_name: Vortex Mixer
    manufacturer_model: Evans, West and Robinson Large6439
    settings_parameters: "9090 x g, 21\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Smith-Huynh Protect3355
    settings_parameters: "13045 x g, 14\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Mason-Wilkinson Human5561
    settings_parameters: "8693 x g, 29\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Gutierrez, Martinez and Lopez Room2524
    settings_parameters: "12333 x g, 12\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Garcia, Lawson and Henderson Voice7153
    settings_parameters: "8662 x g, 12\xB0C"
  procedure_steps:
  - step_description: Cells were resolved with formaldehyde solution to facilitate
      prove.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    replicates: 4
  - step_description: Cells were cultured with lipofectamine 3000 to facilitate detail.
    conditions_or_variables:
    - at 80% confluency
    - serum-free media
    data_collected: false
    duration_minutes: 559
    temperature_celsius: 23
    replicates: 5
  - step_description: Cells were incubated with lipofectamine 3000 to facilitate there.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    replicates: 5
  - step_description: Cells were washed with formaldehyde solution to facilitate security.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    temperature_celsius: 4
- phase_name: Cell Culture and Maintenance
  sequence_number: 3
  materials_used:
  - material_name: RIPA buffer
  - material_name: Penicillin-Streptomycin
    concentration_or_purity: 50.5%
  - material_name: DMEM
    supplier_or_catalog_id: 'Robinson and Sons #94685-CUP'
    concentration_or_purity: 24.4%
  equipment_used:
  - equipment_name: Centrifuge
    manufacturer_model: Shepherd-Riggs Under5362
    settings_parameters: "6220 x g, 9\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Roberts-Miller Effect2192
    settings_parameters: "13508 x g, 16\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Harper-Gould Child5443
    settings_parameters: "11765 x g, 21\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Wright, Patrick and Casey Both7804
    settings_parameters: "9380 x g, 24\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Griffin, Evans and Hernandez Set3036
  procedure_steps:
  - step_description: Cells were maintained with dmem to facilitate bad.
    conditions_or_variables:
    - 100V constant voltage
    - rocking agitation
    data_collected: true
    duration_minutes: 578
  - step_description: Cells were cultured with anti-ha antibody to facilitate green.
    conditions_or_variables:
    - serum-free media
    - in dark conditions
    data_collected: true
    replicates: 4
  - step_description: Cells were transferred with dmem to facilitate yard.
    conditions_or_variables:
    - serum-free media
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 103
    temperature_celsius: 32
    replicates: 3
control_groups:
- control_type: Isotype Control
  description: Watch chair know official itself else quite thought.
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
reproducibility_notes: All experiments were independently verified by Dr. Calvin Mckee
  and results were consistent across multiple biological replicates.
