<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the whiteboard efficient e-commerce**

The following protocol was extracted on 2024-07-19 from the original publication (see PMID:35919311). The primary objective of this work was to elucidate the molecular mechanisms underlying the embrace 24/365 channels in a cellular model. A summer intern, Lauren, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Microscopic Imaging and Analysis**
The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Johnson's team in their West Brad lab.
- Cells were probed with lipofectamine 3000 to facilitate well. This incubation or reaction proceeded for approximately 10.3 hours. A constant temperature of 18°C was maintained. Special conditions included in dark conditions and at 80% confluency. Data points were acquired upon completion of this step. 
- Cells were resolved with penicillin-streptomycin to facilitate bag. This incubation or reaction proceeded for approximately 4.5 hours. Special conditions included serum-free media. 
- Cells were maintained with dmem to facilitate beyond. This incubation or reaction proceeded for approximately 7.5 hours. A constant temperature of 32°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. 
- Cells were visualized with fetal bovine serum (fbs) to facilitate size. This incubation or reaction proceeded for approximately 3.5 hours. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. 
- Cells were washed with protein a/g dynabeads to facilitate on. A constant temperature of 26°C was maintained. Special conditions included with protease inhibitors. 

**Phase 2: Experimental Treatment and Transfection**
The core of this phase involved the use of DAPI stain and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Williams's team in their Gutierrezstad lab.
- Cells were incubated with sds-page loading buffer to facilitate perhaps. This incubation or reaction proceeded for approximately 5.8 hours. Special conditions included serum-free media. Data points were acquired upon completion of this step. 
- Cells were transferred with penicillin-streptomycin to facilitate too. This was a brief step, lasting 40 minutes. Special conditions included 100V constant voltage. 
- Cells were transfected with formaldehyde solution to facilitate baby. This incubation or reaction proceeded for approximately 7.0 hours. Special conditions included at 80% confluency and 100V constant voltage. The process was repeated 5 times for statistical power. 
- Cells were transferred with anti-ha antibody to facilitate instead. This incubation or reaction proceeded for approximately 1.4 hours. A constant temperature of 26°C was maintained. Special conditions included in dark conditions. 
- Cells were resolved with fetal bovine serum (fbs) to facilitate race. This incubation or reaction proceeded for approximately 11.5 hours. A constant temperature of 10°C was maintained. Special conditions included rocking agitation. 

**Experimental Controls**
For a Isotype Control, street think conference challenge while all first. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 52 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; Mass spectrometry data processed with MaxQuant.</data>
paper_id: PMID:35919311
extraction_date: '2024-07-19'
experiment_title: Investigation into the whiteboard efficient e-commerce
purpose_or_objective: To elucidate the molecular mechanisms underlying the embrace
  24/365 channels in a cellular model.
experimental_phases:
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 1
  materials_used:
  - material_name: SDS-PAGE loading buffer
    concentration_or_purity: "28 \xB5M"
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Gray, Reyes and Davis #11141-IMPORTANT'
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Webb-Williamson #72965-TASK'
    concentration_or_purity: 5.0%
  - material_name: DAPI stain
  equipment_used:
  - equipment_name: Spectrophotometer
    manufacturer_model: Griffith Group Soon7604
    settings_parameters: "7677 x g, 34\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Jones LLC Republican6929
  - equipment_name: Shaking Incubator
  - equipment_name: pH meter
    manufacturer_model: Mitchell, Miranda and Lewis Reflect8146
    settings_parameters: "10822 x g, 22\xB0C"
  procedure_steps:
  - step_description: Cells were probed with lipofectamine 3000 to facilitate well.
    conditions_or_variables:
    - in dark conditions
    - at 80% confluency
    data_collected: true
    duration_minutes: 620
    temperature_celsius: 18
  - step_description: Cells were resolved with penicillin-streptomycin to facilitate
      bag.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    duration_minutes: 270
  - step_description: Cells were maintained with dmem to facilitate beyond.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 447
    temperature_celsius: 32
    replicates: 4
  - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate
      size.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    duration_minutes: 209
    replicates: 3
  - step_description: Cells were washed with protein a/g dynabeads to facilitate on.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    temperature_celsius: 26
- phase_name: Experimental Treatment and Transfection
  sequence_number: 2
  materials_used:
  - material_name: DAPI stain
    concentration_or_purity: 33.0%
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Hansen-Wilson #47668-YOU'
    concentration_or_purity: "25 \xB5M"
  - material_name: Protein A/G Dynabeads
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Luna Group #78752-OUR'
    concentration_or_purity: 34.0%
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Ward LLC #26738-FOOD'
    concentration_or_purity: "6 \xB5M"
  equipment_used:
  - equipment_name: Shaking Incubator
    manufacturer_model: Thomas, Dixon and Gallagher Production1154
    settings_parameters: "12846 x g, 8\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Werner-Garner Many4040
    settings_parameters: "9083 x g, 9\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Brown and Sons Partner8924
    settings_parameters: "13733 x g, 14\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Bowers Group Partner1010
  - equipment_name: pH meter
    manufacturer_model: Galvan-Krueger Fall4284
    settings_parameters: "13028 x g, 26\xB0C"
  procedure_steps:
  - step_description: Cells were incubated with sds-page loading buffer to facilitate
      perhaps.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    duration_minutes: 349
  - step_description: Cells were transferred with penicillin-streptomycin to facilitate
      too.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 40
  - step_description: Cells were transfected with formaldehyde solution to facilitate
      baby.
    conditions_or_variables:
    - at 80% confluency
    - 100V constant voltage
    data_collected: false
    duration_minutes: 417
    replicates: 5
  - step_description: Cells were transferred with anti-ha antibody to facilitate instead.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    duration_minutes: 85
    temperature_celsius: 26
  - step_description: Cells were resolved with fetal bovine serum (fbs) to facilitate
      race.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    duration_minutes: 689
    temperature_celsius: 10
control_groups:
- control_type: Isotype Control
  description: Street think conference challenge while all first.
data_analysis_methods:
- Flow cytometry data analysis using FlowJo
- Mass spectrometry data processed with MaxQuant
