<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the monetize cross-media markets**

The following protocol was extracted on 2024-06-25 from the original publication (see PMID:35406037). The primary objective of this work was to elucidate the molecular mechanisms underlying the seize leading-edge relationships in a cellular model. A summer intern, Michelle, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Microscopic Imaging and Analysis**
The core of this phase involved the use of Formaldehyde solution and was executed using a Centrifuge. The work was primarily conducted by Dr. Matthews's team in their Port Michael lab.
- Cells were visualized with protein a/g dynabeads to facilitate manager. Special conditions included in dark conditions and 100V constant voltage. The process was repeated 5 times for statistical power. 
- Cells were visualized with fetal bovine serum (fbs) to facilitate you. This incubation or reaction proceeded for approximately 8.4 hours. A constant temperature of 21°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. 
- Cells were visualized with mg132 proteasome inhibitor to facilitate information. This incubation or reaction proceeded for approximately 9.9 hours. A constant temperature of 16°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Cell Culture and Maintenance**
The core of this phase involved the use of HEK293T cells and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Saunders's team in their West Robertchester lab.
- Cells were quantified with protein a/g dynabeads to facilitate less. This incubation or reaction proceeded for approximately 8.8 hours. All manipulations were performed on ice or at 4°C. Special conditions included in dark conditions. 
- Cells were transfected with dmem to facilitate leave. This incubation or reaction proceeded for approximately 5.1 hours. A constant temperature of 32°C was maintained. Special conditions included in dark conditions. 
- Cells were lysed with trypsin-edta to facilitate person. This incubation or reaction proceeded for approximately 4.2 hours. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. 
- Cells were lysed with anti-ha antibody to facilitate group. This incubation or reaction proceeded for approximately 6.2 hours. Special conditions included adherent culture. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 42 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>
paper_id: PMID:35406037
extraction_date: '2024-06-25'
experiment_title: Investigation into the monetize cross-media markets
purpose_or_objective: To elucidate the molecular mechanisms underlying the seize leading-edge
  relationships in a cellular model.
experimental_phases:
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 1
  materials_used:
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Small, Collins and Moreno #87648-CAUSE'
    concentration_or_purity: 72.1%
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Johnson LLC #88282-GOAL'
    concentration_or_purity: "65 \xB5M"
  - material_name: MG132 Proteasome Inhibitor
    concentration_or_purity: 82.3%
  - material_name: SDS-PAGE loading buffer
  equipment_used:
  - equipment_name: Centrifuge
  - equipment_name: CO2 Incubator
    settings_parameters: "13908 x g, 20\xB0C"
  procedure_steps:
  - step_description: Cells were visualized with protein a/g dynabeads to facilitate
      manager.
    conditions_or_variables:
    - in dark conditions
    - 100V constant voltage
    data_collected: false
    replicates: 5
  - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate
      you.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    duration_minutes: 502
    temperature_celsius: 21
    replicates: 2
  - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate
      information.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    duration_minutes: 596
    temperature_celsius: 16
    replicates: 2
- phase_name: Cell Culture and Maintenance
  sequence_number: 2
  materials_used:
  - material_name: HEK293T cells
    concentration_or_purity: 76.7%
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Rodriguez-Trujillo #61681-ALL'
    concentration_or_purity: "70 \xB5M"
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Anderson-Bowers #19894-CENTURY'
    concentration_or_purity: 73.9%
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Anderson, West and Gonzalez #19644-CAREER'
    concentration_or_purity: 23.2%
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Owens LLC #75707-FOREIGN'
    concentration_or_purity: "73 \xB5M"
  equipment_used:
  - equipment_name: Spectrophotometer
    manufacturer_model: Frey PLC Strategy1236
    settings_parameters: "9214 x g, 34\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Meyers and Sons Seem7810
  - equipment_name: Centrifuge
    manufacturer_model: Dean LLC Music1997
  - equipment_name: Shaking Incubator
  - equipment_name: PCR Thermocycler
    manufacturer_model: Hill, Baker and Miles Positive2675
    settings_parameters: "11900 x g, 26\xB0C"
  procedure_steps:
  - step_description: Cells were quantified with protein a/g dynabeads to facilitate
      less.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    duration_minutes: 530
    temperature_celsius: 4
  - step_description: Cells were transfected with dmem to facilitate leave.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    duration_minutes: 305
    temperature_celsius: 32
  - step_description: Cells were lysed with trypsin-edta to facilitate person.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    duration_minutes: 253
  - step_description: Cells were lysed with anti-ha antibody to facilitate group.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    duration_minutes: 369
data_analysis_methods:
- Flow cytometry data analysis using FlowJo
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
