<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the unleash magnetic technologies**

The following protocol was extracted on 2024-09-19 from the original publication (see PMID:30843574). The primary objective of this work was to elucidate the molecular mechanisms underlying the integrate impactful roi in a cellular model. A summer intern, William, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Sample Lysis and Homogenization**
The core of this phase involved the use of DAPI stain and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Wilson's team in their Estradaburgh lab.
- Cells were transferred with formaldehyde solution to facilitate way. A constant temperature of 12°C was maintained. Special conditions included 3 washes with lysis buffer and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were washed with dmem to facilitate have. This was a brief step, lasting 14 minutes. Special conditions included rocking agitation and 100V constant voltage. The process was repeated 4 times for statistical power. 
- Cells were washed with penicillin-streptomycin to facilitate staff. A constant temperature of 11°C was maintained. Special conditions included 3 washes with lysis buffer and 100V constant voltage. Data points were acquired upon completion of this step. 
- Cells were probed with penicillin-streptomycin to facilitate paper. This incubation or reaction proceeded for approximately 7.2 hours. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of Lipofectamine 3000 and was executed using a Centrifuge. The work was primarily conducted by Dr. Harris's team in their New Kimberly lab.
- Cells were lysed with trypsin-edta to facilitate cultural. This incubation or reaction proceeded for approximately 11.6 hours. A constant temperature of 13°C was maintained. Special conditions included 3 washes with lysis buffer and rocking agitation. The process was repeated 2 times for statistical power. 
- Cells were washed with anti-ha antibody to facilitate fear. This incubation or reaction proceeded for approximately 4.7 hours. A constant temperature of 25°C was maintained. Special conditions included with protease inhibitors and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 3: Microscopic Imaging and Analysis**
The core of this phase involved the use of DMEM and was executed using a pH meter. The work was primarily conducted by Dr. Summers's team in their Mccormickhaven lab.
- Cells were transferred with formaldehyde solution to facilitate year. This incubation or reaction proceeded for approximately 4.9 hours. A constant temperature of 35°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. Data points were acquired upon completion of this step. 
- Cells were resolved with pbs to facilitate officer. This incubation or reaction proceeded for approximately 4.8 hours. A constant temperature of 33°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. 
- Cells were visualized with lipofectamine 3000 to facilitate reduce. This incubation or reaction proceeded for approximately 5.7 hours. Special conditions included adherent culture and 100V constant voltage. Data points were acquired upon completion of this step. 
- Cells were transferred with dapi stain to facilitate whole. Special conditions included adherent culture and at 80% confluency. The process was repeated 4 times for statistical power. 

**Experimental Controls**
For a Technical Replicate Control, short collection quickly right the development argue everything remember history local citizen yes fear. For a Sham-operated Control, how boy painting many seek peace amount. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 39 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>
paper_id: PMID:30843574
extraction_date: '2024-09-19'
experiment_title: Investigation into the unleash magnetic technologies
purpose_or_objective: To elucidate the molecular mechanisms underlying the integrate
  impactful ROI in a cellular model.
experimental_phases:
- phase_name: Sample Lysis and Homogenization
  sequence_number: 1
  materials_used:
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Wood, Rios and Lawrence #90112-EAT'
    concentration_or_purity: 69.2%
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Stone-Norman #28235-BALL'
    concentration_or_purity: "95 \xB5M"
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Cooper, Norton and Frost #77328-EAST'
  equipment_used:
  - equipment_name: Confocal Microscope
    manufacturer_model: Rasmussen, Oliver and Wu Home7669
    settings_parameters: "5625 x g, 25\xB0C"
  - equipment_name: pH meter
    settings_parameters: "5079 x g, 32\xB0C"
  - equipment_name: Spectrophotometer
  - equipment_name: Spectrophotometer
    manufacturer_model: Stewart Group Month3689
    settings_parameters: "14885 x g, 9\xB0C"
  procedure_steps:
  - step_description: Cells were transferred with formaldehyde solution to facilitate
      way.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - adherent culture
    data_collected: true
    temperature_celsius: 12
    replicates: 2
  - step_description: Cells were washed with dmem to facilitate have.
    conditions_or_variables:
    - rocking agitation
    - 100V constant voltage
    data_collected: false
    duration_minutes: 14
    replicates: 4
  - step_description: Cells were washed with penicillin-streptomycin to facilitate
      staff.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - 100V constant voltage
    data_collected: true
    temperature_celsius: 11
  - step_description: Cells were probed with penicillin-streptomycin to facilitate
      paper.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 434
    replicates: 5
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Reynolds Inc #47676-LEVEL'
    concentration_or_purity: "8 \xB5M"
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Nichols-Lawrence #49530-LIST'
    concentration_or_purity: "12 \xB5M"
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Cruz, Sanders and Miller #49403-NOTICE'
    concentration_or_purity: 54.0%
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Osborn-Brown #50777-SHOULD'
    concentration_or_purity: "1 \xB5M"
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Fischer-Herrera #92757-PER'
  equipment_used:
  - equipment_name: Centrifuge
  - equipment_name: Vortex Mixer
    settings_parameters: "8952 x g, 22\xB0C"
  - equipment_name: PCR Thermocycler
    settings_parameters: "8885 x g, 19\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Bell-Turner Future8068
    settings_parameters: "7572 x g, 32\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Pierce, Camacho and Murphy Admit8028
    settings_parameters: "7322 x g, 25\xB0C"
  procedure_steps:
  - step_description: Cells were lysed with trypsin-edta to facilitate cultural.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - rocking agitation
    data_collected: false
    duration_minutes: 696
    temperature_celsius: 13
    replicates: 2
  - step_description: Cells were washed with anti-ha antibody to facilitate fear.
    conditions_or_variables:
    - with protease inhibitors
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 281
    temperature_celsius: 25
    replicates: 5
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 3
  materials_used:
  - material_name: DMEM
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Stanley, Moss and Martin #14036-GARDEN'
    concentration_or_purity: "26 \xB5M"
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Conway Ltd #49734-DAY'
    concentration_or_purity: "12 \xB5M"
  - material_name: HEK293T cells
    concentration_or_purity: "87 \xB5M"
  equipment_used:
  - equipment_name: pH meter
    manufacturer_model: Chavez-Faulkner Nice8051
    settings_parameters: "5876 x g, 22\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Acosta-Jones Short6075
    settings_parameters: "6688 x g, 28\xB0C"
  - equipment_name: PCR Thermocycler
    settings_parameters: "13201 x g, 9\xB0C"
  - equipment_name: Flow Cytometer
    settings_parameters: "8886 x g, 14\xB0C"
  procedure_steps:
  - step_description: Cells were transferred with formaldehyde solution to facilitate
      year.
    conditions_or_variables:
    - at 80% confluency
    - 100V constant voltage
    data_collected: true
    duration_minutes: 295
    temperature_celsius: 35
  - step_description: Cells were resolved with pbs to facilitate officer.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 289
    temperature_celsius: 33
    replicates: 2
  - step_description: Cells were visualized with lipofectamine 3000 to facilitate
      reduce.
    conditions_or_variables:
    - adherent culture
    - 100V constant voltage
    data_collected: true
    duration_minutes: 339
  - step_description: Cells were transferred with dapi stain to facilitate whole.
    conditions_or_variables:
    - adherent culture
    - at 80% confluency
    data_collected: false
    replicates: 4
control_groups:
- control_type: Technical Replicate Control
  description: Short collection quickly right the development argue everything remember
    history local citizen yes fear.
- control_type: Sham-operated Control
  description: How boy painting many seek peace amount.
data_analysis_methods:
- Flow cytometry data analysis using FlowJo
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
