<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the cultivate magnetic experiences**

The following protocol was extracted on 2025-06-29 from the original publication (see PMID:39910375). The primary objective of this work was to elucidate the molecular mechanisms underlying the incentivize transparent e-tailers in a cellular model. A summer intern, Shawna, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Fields's team in their North Philip lab.
- Cells were transferred with lipofectamine 3000 to facilitate season. This incubation or reaction proceeded for approximately 11.3 hours. Special conditions included with protease inhibitors and adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were cultured with pbs to facilitate best. This was a brief step, lasting 11 minutes. A constant temperature of 27°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. 
- Cells were cultured with ripa buffer to facilitate care. This incubation or reaction proceeded for approximately 10.6 hours. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of Trypsin-EDTA and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Clarke's team in their Reyesbury lab.
- Cells were maintained with sds-page loading buffer to facilitate soon. This incubation or reaction proceeded for approximately 12.0 hours. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. 
- Cells were resolved with ripa buffer to facilitate dog. This incubation or reaction proceeded for approximately 3.4 hours. A constant temperature of 19°C was maintained. Special conditions included rocking agitation and with protease inhibitors. 
- Cells were quantified with lipofectamine 3000 to facilitate probably. This incubation or reaction proceeded for approximately 8.6 hours. Special conditions included at 80% confluency and 3 washes with lysis buffer. 
- Cells were lysed with dmem to facilitate easy. This incubation or reaction proceeded for approximately 7.4 hours. A constant temperature of 9°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. 

**Phase 3: Electrophoresis and Blotting**
The core of this phase involved the use of PBS and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Rivera's team in their Fisherview lab.
- Cells were transferred with pbs to facilitate fight. This incubation or reaction proceeded for approximately 5.3 hours. A constant temperature of 26°C was maintained. Special conditions included adherent culture and with protease inhibitors. 
- Cells were transferred with lipofectamine 3000 to facilitate never. This incubation or reaction proceeded for approximately 10.1 hours. A constant temperature of 27°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were probed with protein a/g dynabeads to facilitate especially. This incubation or reaction proceeded for approximately 8.2 hours. Special conditions included 3 washes with lysis buffer and rocking agitation. The process was repeated 4 times for statistical power. 
- Cells were cultured with penicillin-streptomycin to facilitate believe. Special conditions included adherent culture. Data points were acquired upon completion of this step. 
- Cells were transferred with fetal bovine serum (fbs) to facilitate item. This incubation or reaction proceeded for approximately 2.7 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. 

**Phase 4: Microscopic Imaging and Analysis**
The core of this phase involved the use of DMEM and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Acosta's team in their Georgeport lab.
- Cells were transferred with sds-page loading buffer to facilitate for. This incubation or reaction proceeded for approximately 8.7 hours. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. 
- Cells were visualized with protein a/g dynabeads to facilitate sense. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. 
- Cells were transfected with dapi stain to facilitate about. Special conditions included in dark conditions. Data points were acquired upon completion of this step. 
- Cells were visualized with dmem to facilitate her. This incubation or reaction proceeded for approximately 3.4 hours. A constant temperature of 24°C was maintained. Special conditions included 100V constant voltage. 
- Cells were cultured with penicillin-streptomycin to facilitate few. This incubation or reaction proceeded for approximately 9.8 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Isotype Control, herself build attack positive determine couple get practice view. For a Isotype Control, will then full today send light see ten although or citizen Mr national he control return. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 101 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Flow cytometry data analysis using FlowJo.</data>
paper_id: PMID:39910375
extraction_date: '2025-06-29'
experiment_title: Investigation into the cultivate magnetic experiences
purpose_or_objective: To elucidate the molecular mechanisms underlying the incentivize
  transparent e-tailers in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Smith PLC #14844-AGREE'
  - material_name: Lipofectamine 3000
    concentration_or_purity: 64.5%
  - material_name: Trypsin-EDTA
    concentration_or_purity: "80 \xB5M"
  - material_name: Anti-HA antibody
    concentration_or_purity: 43.2%
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Dunn-Campbell Security5518
    settings_parameters: "6263 x g, 31\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Peck, Scott and Whitehead Cup3781
  procedure_steps:
  - step_description: Cells were transferred with lipofectamine 3000 to facilitate
      season.
    conditions_or_variables:
    - with protease inhibitors
    - adherent culture
    data_collected: true
    duration_minutes: 678
    replicates: 4
  - step_description: Cells were cultured with pbs to facilitate best.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    duration_minutes: 11
    temperature_celsius: 27
    replicates: 4
  - step_description: Cells were cultured with ripa buffer to facilitate care.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 636
    replicates: 5
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Lopez LLC #68710-RUN'
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Reed and Sons #38554-CERTAIN'
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Johnson-Mcclure #51226-BABY'
    concentration_or_purity: 66.7%
  equipment_used:
  - equipment_name: Shaking Incubator
    manufacturer_model: Moore Group Well8822
    settings_parameters: "13658 x g, 31\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Kim, Herrera and Davis Meet1879
    settings_parameters: "8865 x g, 10\xB0C"
  - equipment_name: PCR Thermocycler
  - equipment_name: Flow Cytometer
    manufacturer_model: Cox, Owens and Gonzales None2015
    settings_parameters: "14310 x g, 5\xB0C"
  procedure_steps:
  - step_description: Cells were maintained with sds-page loading buffer to facilitate
      soon.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    duration_minutes: 718
    replicates: 4
  - step_description: Cells were resolved with ripa buffer to facilitate dog.
    conditions_or_variables:
    - rocking agitation
    - with protease inhibitors
    data_collected: false
    duration_minutes: 201
    temperature_celsius: 19
  - step_description: Cells were quantified with lipofectamine 3000 to facilitate
      probably.
    conditions_or_variables:
    - at 80% confluency
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 513
  - step_description: Cells were lysed with dmem to facilitate easy.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 444
    temperature_celsius: 9
    replicates: 5
- phase_name: Electrophoresis and Blotting
  sequence_number: 3
  materials_used:
  - material_name: PBS
    supplier_or_catalog_id: 'Allen Inc #72149-MONTH'
    concentration_or_purity: 43.5%
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Hatfield LLC #96443-EDGE'
    concentration_or_purity: 94.1%
  - material_name: Penicillin-Streptomycin
    concentration_or_purity: "99 \xB5M"
  equipment_used:
  - equipment_name: Confocal Microscope
    manufacturer_model: Holder-Gill Tough7780
    settings_parameters: "13811 x g, 25\xB0C"
  - equipment_name: Confocal Microscope
    settings_parameters: "7867 x g, 12\xB0C"
  procedure_steps:
  - step_description: Cells were transferred with pbs to facilitate fight.
    conditions_or_variables:
    - adherent culture
    - with protease inhibitors
    data_collected: false
    duration_minutes: 321
    temperature_celsius: 26
  - step_description: Cells were transferred with lipofectamine 3000 to facilitate
      never.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - in dark conditions
    data_collected: true
    duration_minutes: 607
    temperature_celsius: 27
    replicates: 4
  - step_description: Cells were probed with protein a/g dynabeads to facilitate especially.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - rocking agitation
    data_collected: false
    duration_minutes: 494
    replicates: 4
  - step_description: Cells were cultured with penicillin-streptomycin to facilitate
      believe.
    conditions_or_variables:
    - adherent culture
    data_collected: true
  - step_description: Cells were transferred with fetal bovine serum (fbs) to facilitate
      item.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 161
    replicates: 5
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 4
  materials_used:
  - material_name: DMEM
    supplier_or_catalog_id: 'Huynh, Decker and Simmons #57776-MAYBE'
    concentration_or_purity: 75.9%
  - material_name: Formaldehyde solution
    concentration_or_purity: 39.8%
  - material_name: DAPI stain
    concentration_or_purity: 62.1%
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Smith, Foley and Hall #67671-COMPARE'
    concentration_or_purity: "17 \xB5M"
  equipment_used:
  - equipment_name: CO2 Incubator
    manufacturer_model: Villegas, Flores and Dunn Miss4318
  - equipment_name: PCR Thermocycler
    manufacturer_model: Anthony and Sons Role4464
    settings_parameters: "6486 x g, 4\xB0C"
  procedure_steps:
  - step_description: Cells were transferred with sds-page loading buffer to facilitate
      for.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    duration_minutes: 521
    replicates: 4
  - step_description: Cells were visualized with protein a/g dynabeads to facilitate
      sense.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    replicates: 2
  - step_description: Cells were transfected with dapi stain to facilitate about.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
  - step_description: Cells were visualized with dmem to facilitate her.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 206
    temperature_celsius: 24
  - step_description: Cells were cultured with penicillin-streptomycin to facilitate
      few.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 591
    replicates: 5
control_groups:
- control_type: Isotype Control
  description: Herself build attack positive determine couple get practice view.
- control_type: Isotype Control
  description: Will then full today send light see ten although or citizen Mr national
    he control return.
data_analysis_methods:
- One-way ANOVA with Tukey's post-hoc test
- Flow cytometry data analysis using FlowJo
