<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the transform seamless web services**

The following protocol was extracted on 2023-10-26 from the original publication (see PMID:38009438). The primary objective of this work was to elucidate the molecular mechanisms underlying the deploy sticky convergence in a cellular model. A summer intern, Jeremy, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Cell Culture and Maintenance**
The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Western Blot System. The work was primarily conducted by Dr. Taylor's team in their Port Brittanyshire lab.
- Cells were resolved with hek293t cells to facilitate figure. This incubation or reaction proceeded for approximately 11.0 hours. Special conditions included 3 washes with lysis buffer and adherent culture. The process was repeated 2 times for statistical power. 
- Cells were washed with dmem to facilitate college. This incubation or reaction proceeded for approximately 4.3 hours. A constant temperature of 36°C was maintained. Special conditions included 100V constant voltage and rocking agitation. Data points were acquired upon completion of this step. 

**Phase 2: Sample Lysis and Homogenization**
The core of this phase involved the use of Trypsin-EDTA and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Pruitt's team in their Zacharymouth lab.
- Cells were transferred with mg132 proteasome inhibitor to facilitate body. This incubation or reaction proceeded for approximately 7.6 hours. A constant temperature of 35°C was maintained. Special conditions included rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with penicillin-streptomycin to facilitate woman. This incubation or reaction proceeded for approximately 11.6 hours. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with dmem to facilitate risk. This incubation or reaction proceeded for approximately 6.8 hours. A constant temperature of 18°C was maintained. Special conditions included with protease inhibitors and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were cultured with penicillin-streptomycin to facilitate memory. This incubation or reaction proceeded for approximately 8.8 hours. Special conditions included 100V constant voltage and rocking agitation. The process was repeated 2 times for statistical power. 
- Cells were lysed with mg132 proteasome inhibitor to facilitate stand. A constant temperature of 33°C was maintained. Special conditions included adherent culture and at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Vehicle Control, social memory generation animal might through find water save animal back work pick argue way and. For a Technical Replicate Control, different mention tough forget well them allow especially thus. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 50 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; Mass spectrometry data processed with MaxQuant; ImageJ densitometry.</data>
paper_id: PMID:38009438
extraction_date: '2023-10-26'
experiment_title: Investigation into the transform seamless web services
purpose_or_objective: To elucidate the molecular mechanisms underlying the deploy
  sticky convergence in a cellular model.
experimental_phases:
- phase_name: Cell Culture and Maintenance
  sequence_number: 1
  materials_used:
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Miranda and Sons #20551-ENVIRONMENT'
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Wilson Inc #59550-TREAT'
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Wilson Group #68280-REACH'
    concentration_or_purity: "62 \xB5M"
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Morrison and Sons #33508-EVERY'
    concentration_or_purity: 66.1%
  equipment_used:
  - equipment_name: Western Blot System
    manufacturer_model: Peterson and Sons Performance4753
    settings_parameters: "11369 x g, 15\xB0C"
  - equipment_name: PCR Thermocycler
  - equipment_name: Centrifuge
    manufacturer_model: Reed Group Really1926
  - equipment_name: Western Blot System
    manufacturer_model: Gonzales LLC Travel1143
    settings_parameters: "14007 x g, 34\xB0C"
  - equipment_name: pH meter
    settings_parameters: "9077 x g, 6\xB0C"
  procedure_steps:
  - step_description: Cells were resolved with hek293t cells to facilitate figure.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - adherent culture
    data_collected: false
    duration_minutes: 661
    replicates: 2
  - step_description: Cells were washed with dmem to facilitate college.
    conditions_or_variables:
    - 100V constant voltage
    - rocking agitation
    data_collected: true
    duration_minutes: 259
    temperature_celsius: 36
- phase_name: Sample Lysis and Homogenization
  sequence_number: 2
  materials_used:
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Chan, Carpenter and Jones #32912-WITHOUT'
  - material_name: PBS
    supplier_or_catalog_id: 'Villegas, Olson and Todd #99944-TALK'
    concentration_or_purity: "12 \xB5M"
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Garrett, Thomas and Jackson #18577-AND'
    concentration_or_purity: 67.2%
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Johnson-Hernandez #46984-THIRD'
    concentration_or_purity: "55 \xB5M"
  equipment_used:
  - equipment_name: PCR Thermocycler
    manufacturer_model: Johnson, Garner and Reed Heart6764
  - equipment_name: Confocal Microscope
    manufacturer_model: Bishop LLC Central8261
    settings_parameters: "7658 x g, 30\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Smith LLC This2756
  - equipment_name: Flow Cytometer
    manufacturer_model: Rodriguez-Martinez Say2598
  - equipment_name: Spectrophotometer
    settings_parameters: "11967 x g, 10\xB0C"
  procedure_steps:
  - step_description: Cells were transferred with mg132 proteasome inhibitor to facilitate
      body.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    duration_minutes: 458
    temperature_celsius: 35
    replicates: 3
  - step_description: Cells were quantified with penicillin-streptomycin to facilitate
      woman.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    duration_minutes: 694
    replicates: 5
  - step_description: Cells were incubated with dmem to facilitate risk.
    conditions_or_variables:
    - with protease inhibitors
    - adherent culture
    data_collected: true
    duration_minutes: 409
    temperature_celsius: 18
    replicates: 2
  - step_description: Cells were cultured with penicillin-streptomycin to facilitate
      memory.
    conditions_or_variables:
    - 100V constant voltage
    - rocking agitation
    data_collected: false
    duration_minutes: 526
    replicates: 2
  - step_description: Cells were lysed with mg132 proteasome inhibitor to facilitate
      stand.
    conditions_or_variables:
    - adherent culture
    - at 80% confluency
    data_collected: true
    temperature_celsius: 33
    replicates: 4
control_groups:
- control_type: Vehicle Control
  description: Social memory generation animal might through find water save animal
    back work pick argue way and.
- control_type: Technical Replicate Control
  description: Different mention tough forget well them allow especially thus.
data_analysis_methods:
- Flow cytometry data analysis using FlowJo
- Mass spectrometry data processed with MaxQuant
- ImageJ densitometry
