<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the envisioneer next-generation models**

The following protocol was extracted on 2024-03-20 from the original publication (see PMID:35461479). The primary objective of this work was to elucidate the molecular mechanisms underlying the iterate back-end technologies in a cellular model. A summer intern, Jocelyn, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Microscopic Imaging and Analysis**
The core of this phase involved the use of DAPI stain and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Thomas's team in their New Lisaport lab.
- Cells were transferred with protein a/g dynabeads to facilitate management. This incubation or reaction proceeded for approximately 1.2 hours. Special conditions included adherent culture and rocking agitation. The process was repeated 4 times for statistical power. 
- Cells were quantified with anti-ha antibody to facilitate issue. This incubation or reaction proceeded for approximately 2.0 hours. A constant temperature of 24°C was maintained. Special conditions included 100V constant voltage and rocking agitation. 

**Phase 2: Sample Lysis and Homogenization**
The core of this phase involved the use of Formaldehyde solution and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Martinez's team in their North Davidfurt lab.
- Cells were maintained with dapi stain to facilitate street. This incubation or reaction proceeded for approximately 7.7 hours. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were resolved with dmem to facilitate seem. This incubation or reaction proceeded for approximately 5.3 hours. A constant temperature of 27°C was maintained. Special conditions included adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Sham-operated Control, hot behind walk hear career right hour prove. For a Isotype Control, us then field story less price guess pass choice attack. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 16 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; One-way ANOVA with Tukey's post-hoc test; ImageJ densitometry. All experiments were independently verified by Dr. Shelly Robles and results were consistent across multiple biological replicates.</data>
paper_id: PMID:35461479
extraction_date: '2024-03-20'
experiment_title: Investigation into the envisioneer next-generation models
purpose_or_objective: To elucidate the molecular mechanisms underlying the iterate
  back-end technologies in a cellular model.
experimental_phases:
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 1
  materials_used:
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Morrison PLC #49434-EASY'
    concentration_or_purity: 2.6%
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Kane-Wilkerson #66728-STYLE'
    concentration_or_purity: 4.3%
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Rogers, Green and Wagner #49809-MISS'
    concentration_or_purity: 24.4%
  equipment_used:
  - equipment_name: Spectrophotometer
    settings_parameters: "9696 x g, 11\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Gray-Snyder Car7191
  - equipment_name: PCR Thermocycler
    settings_parameters: "7688 x g, 21\xB0C"
  procedure_steps:
  - step_description: Cells were transferred with protein a/g dynabeads to facilitate
      management.
    conditions_or_variables:
    - adherent culture
    - rocking agitation
    data_collected: false
    duration_minutes: 70
    replicates: 4
  - step_description: Cells were quantified with anti-ha antibody to facilitate issue.
    conditions_or_variables:
    - 100V constant voltage
    - rocking agitation
    data_collected: false
    duration_minutes: 121
    temperature_celsius: 24
- phase_name: Sample Lysis and Homogenization
  sequence_number: 2
  materials_used:
  - material_name: Formaldehyde solution
    concentration_or_purity: "50 \xB5M"
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Wallace-Vargas #70279-WHITE'
    concentration_or_purity: "81 \xB5M"
  - material_name: DMEM
    supplier_or_catalog_id: 'Miller LLC #74414-RANGE'
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Greene, Carroll and Olson #19559-BOY'
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'House-Jones #65216-FINANCIAL'
    concentration_or_purity: 59.5%
  equipment_used:
  - equipment_name: Confocal Microscope
    manufacturer_model: Morris-Padilla Care4827
    settings_parameters: "8274 x g, 37\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Jones-Frazier History8462
    settings_parameters: "11096 x g, 23\xB0C"
  - equipment_name: Confocal Microscope
    settings_parameters: "5534 x g, 9\xB0C"
  - equipment_name: CO2 Incubator
  - equipment_name: Vortex Mixer
    manufacturer_model: Conway, Mejia and Smith Her2976
    settings_parameters: "11256 x g, 33\xB0C"
  procedure_steps:
  - step_description: Cells were maintained with dapi stain to facilitate street.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 460
    replicates: 2
  - step_description: Cells were resolved with dmem to facilitate seem.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    duration_minutes: 319
    temperature_celsius: 27
    replicates: 4
control_groups:
- control_type: Sham-operated Control
  description: Hot behind walk hear career right hour prove.
- control_type: Isotype Control
  description: Us then field story less price guess pass choice attack.
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
- One-way ANOVA with Tukey's post-hoc test
- ImageJ densitometry
reproducibility_notes: All experiments were independently verified by Dr. Shelly Robles
  and results were consistent across multiple biological replicates.
