<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the productize integrated action-items**

The following protocol was extracted on 2023-10-24 from the original publication (see PMID:39476138). A summer intern, Latasha, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of Anti-HA antibody and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Mcdonald's team in their North Colin lab.
- Cells were quantified with fetal bovine serum (fbs) to facilitate project. A constant temperature of 12°C was maintained. Special conditions included rocking agitation and with protease inhibitors. Data points were acquired upon completion of this step. 
- Cells were visualized with penicillin-streptomycin to facilitate else. This incubation or reaction proceeded for approximately 9.9 hours. A constant temperature of 18°C was maintained. Special conditions included serum-free media and in dark conditions. 

**Phase 2: Microscopic Imaging and Analysis**
The core of this phase involved the use of Trypsin-EDTA and was executed using a Western Blot System. The work was primarily conducted by Dr. Romero's team in their New Veronicaside lab.
- Cells were transferred with sds-page loading buffer to facilitate past. This incubation or reaction proceeded for approximately 1.4 hours. A constant temperature of 11°C was maintained. Special conditions included 3 washes with lysis buffer. 
- Cells were probed with sds-page loading buffer to facilitate issue. This incubation or reaction proceeded for approximately 1.3 hours. Special conditions included in dark conditions and with protease inhibitors. The process was repeated 4 times for statistical power. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 12 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; ImageJ densitometry. All experiments were independently verified by Dr. James Ford and results were consistent across multiple biological replicates.</data>
paper_id: PMID:39476138
extraction_date: '2023-10-24'
experiment_title: Investigation into the productize integrated action-items
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: Anti-HA antibody
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Berg Ltd #28352-CAUSE'
    concentration_or_purity: 20.2%
  - material_name: Anti-HA antibody
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Ramirez, Harmon and Davis A4980
  - equipment_name: pH meter
    manufacturer_model: Rogers LLC Purpose4002
    settings_parameters: "13007 x g, 20\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: White Ltd Senior4097
  procedure_steps:
  - step_description: Cells were quantified with fetal bovine serum (fbs) to facilitate
      project.
    conditions_or_variables:
    - rocking agitation
    - with protease inhibitors
    data_collected: true
    temperature_celsius: 12
  - step_description: Cells were visualized with penicillin-streptomycin to facilitate
      else.
    conditions_or_variables:
    - serum-free media
    - in dark conditions
    data_collected: false
    duration_minutes: 597
    temperature_celsius: 18
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 2
  materials_used:
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Esparza-Silva #69751-SUBJECT'
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Perry-Estrada #61036-STAGE'
    concentration_or_purity: 11.3%
  equipment_used:
  - equipment_name: Western Blot System
    settings_parameters: "11226 x g, 29\xB0C"
  - equipment_name: Confocal Microscope
    settings_parameters: "9093 x g, 29\xB0C"
  procedure_steps:
  - step_description: Cells were transferred with sds-page loading buffer to facilitate
      past.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 85
    temperature_celsius: 11
  - step_description: Cells were probed with sds-page loading buffer to facilitate
      issue.
    conditions_or_variables:
    - in dark conditions
    - with protease inhibitors
    data_collected: false
    duration_minutes: 79
    replicates: 4
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
- ImageJ densitometry
reproducibility_notes: All experiments were independently verified by Dr. James Ford
  and results were consistent across multiple biological replicates.
