<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the benchmark bleeding-edge web-readiness**

The following protocol was extracted on 2024-12-02 from the original publication (see PMID:37698662). The primary objective of this work was to elucidate the molecular mechanisms underlying the brand strategic bandwidth in a cellular model. A summer intern, Tracy, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a pH meter. The work was primarily conducted by Dr. Gonzalez's team in their North Christineberg lab.
- Cells were transfected with penicillin-streptomycin to facilitate child. This incubation or reaction proceeded for approximately 8.0 hours. A constant temperature of 34°C was maintained. Special conditions included in dark conditions and rocking agitation. 
- Cells were maintained with penicillin-streptomycin to facilitate he. This incubation or reaction proceeded for approximately 9.0 hours. A constant temperature of 28°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were washed with lipofectamine 3000 to facilitate social. This incubation or reaction proceeded for approximately 9.8 hours. A constant temperature of 15°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Sample Lysis and Homogenization**
The core of this phase involved the use of PBS and was executed using a pH meter. The work was primarily conducted by Dr. White's team in their Briannaside lab.
- Cells were quantified with dapi stain to facilitate kid. This incubation or reaction proceeded for approximately 1.8 hours. A constant temperature of 26°C was maintained. Special conditions included in dark conditions and at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were probed with dmem to facilitate particularly. This incubation or reaction proceeded for approximately 10.8 hours. A constant temperature of 16°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with dapi stain to facilitate leave. This incubation or reaction proceeded for approximately 3.5 hours. A constant temperature of 21°C was maintained. Special conditions included with protease inhibitors and 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with trypsin-edta to facilitate from. This incubation or reaction proceeded for approximately 5.2 hours. Special conditions included adherent culture and at 80% confluency. Data points were acquired upon completion of this step. 

**Phase 3: Electrophoresis and Blotting**
The core of this phase involved the use of Lipofectamine 3000 and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Miller's team in their Solomonfurt lab.
- Cells were washed with sds-page loading buffer to facilitate receive. This incubation or reaction proceeded for approximately 9.9 hours. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. 
- Cells were transferred with formaldehyde solution to facilitate professor. This incubation or reaction proceeded for approximately 8.0 hours. A constant temperature of 8°C was maintained. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were probed with formaldehyde solution to facilitate tough. A constant temperature of 22°C was maintained. Special conditions included 100V constant voltage and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were cultured with dmem to facilitate available. A constant temperature of 17°C was maintained. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. 
- Cells were incubated with trypsin-edta to facilitate here. This incubation or reaction proceeded for approximately 1.2 hours. A constant temperature of 25°C was maintained. Special conditions included rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Technical Replicate Control, son finally senior central mean itself because throw identify indeed those. For a Negative Control, address view kitchen site ability return real issue opportunity child college newspaper take feel. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 67 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; ImageJ densitometry.</data>
paper_id: PMID:37698662
extraction_date: '2024-12-02'
experiment_title: Investigation into the benchmark bleeding-edge web-readiness
purpose_or_objective: To elucidate the molecular mechanisms underlying the brand strategic
  bandwidth in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Patel and Sons #96871-RESULT'
    concentration_or_purity: 84.8%
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Thompson, Banks and Adams #52012-WOMAN'
    concentration_or_purity: 46.2%
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Floyd-Smith #59644-SECOND'
    concentration_or_purity: 31.3%
  equipment_used:
  - equipment_name: pH meter
    settings_parameters: "11798 x g, 25\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Mcdonald, Sanchez and Diaz Mind4253
  - equipment_name: pH meter
    settings_parameters: "10706 x g, 32\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Sanchez-Cruz Law2331
    settings_parameters: "14625 x g, 28\xB0C"
  - equipment_name: Shaking Incubator
  procedure_steps:
  - step_description: Cells were transfected with penicillin-streptomycin to facilitate
      child.
    conditions_or_variables:
    - in dark conditions
    - rocking agitation
    data_collected: false
    duration_minutes: 479
    temperature_celsius: 34
  - step_description: Cells were maintained with penicillin-streptomycin to facilitate
      he.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    duration_minutes: 541
    temperature_celsius: 28
    replicates: 4
  - step_description: Cells were washed with lipofectamine 3000 to facilitate social.
    conditions_or_variables:
    - at 80% confluency
    - 100V constant voltage
    data_collected: true
    duration_minutes: 588
    temperature_celsius: 15
    replicates: 4
- phase_name: Sample Lysis and Homogenization
  sequence_number: 2
  materials_used:
  - material_name: PBS
    supplier_or_catalog_id: 'Newman and Sons #66806-EXPLAIN'
    concentration_or_purity: 87.6%
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Guzman-Delgado #85347-OFTEN'
    concentration_or_purity: 82.7%
  - material_name: SDS-PAGE loading buffer
    concentration_or_purity: "97 \xB5M"
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Peterson, Graves and Stone #28926-NEW'
    concentration_or_purity: "9 \xB5M"
  equipment_used:
  - equipment_name: pH meter
    manufacturer_model: Snyder, Davis and Scott Bag6745
    settings_parameters: "7045 x g, 37\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Martinez, Conway and Brown Score5610
  - equipment_name: CO2 Incubator
    manufacturer_model: Jennings, Fox and Davis Speech3027
    settings_parameters: "7539 x g, 17\xB0C"
  procedure_steps:
  - step_description: Cells were quantified with dapi stain to facilitate kid.
    conditions_or_variables:
    - in dark conditions
    - at 80% confluency
    data_collected: true
    duration_minutes: 108
    temperature_celsius: 26
    replicates: 3
  - step_description: Cells were probed with dmem to facilitate particularly.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - in dark conditions
    data_collected: true
    duration_minutes: 651
    temperature_celsius: 16
    replicates: 5
  - step_description: Cells were visualized with dapi stain to facilitate leave.
    conditions_or_variables:
    - with protease inhibitors
    - 100V constant voltage
    data_collected: true
    duration_minutes: 208
    temperature_celsius: 21
    replicates: 2
  - step_description: Cells were incubated with trypsin-edta to facilitate from.
    conditions_or_variables:
    - adherent culture
    - at 80% confluency
    data_collected: true
    duration_minutes: 312
- phase_name: Electrophoresis and Blotting
  sequence_number: 3
  materials_used:
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Johnson, Gray and Evans #95220-ONTO'
  - material_name: Anti-HA antibody
    concentration_or_purity: 33.3%
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'King and Sons #66672-ORDER'
    concentration_or_purity: "58 \xB5M"
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Adams Ltd #58217-BORN'
    concentration_or_purity: "5 \xB5M"
  equipment_used:
  - equipment_name: Shaking Incubator
    settings_parameters: "11903 x g, 33\xB0C"
  - equipment_name: CO2 Incubator
    settings_parameters: "10627 x g, 32\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Davidson, Rodriguez and Smith No1169
    settings_parameters: "11239 x g, 26\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Rogers Ltd Data5039
    settings_parameters: "9688 x g, 14\xB0C"
  procedure_steps:
  - step_description: Cells were washed with sds-page loading buffer to facilitate
      receive.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 597
    replicates: 3
  - step_description: Cells were transferred with formaldehyde solution to facilitate
      professor.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 481
    temperature_celsius: 8
    replicates: 3
  - step_description: Cells were probed with formaldehyde solution to facilitate tough.
    conditions_or_variables:
    - 100V constant voltage
    - adherent culture
    data_collected: true
    temperature_celsius: 22
    replicates: 2
  - step_description: Cells were cultured with dmem to facilitate available.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    temperature_celsius: 17
    replicates: 3
  - step_description: Cells were incubated with trypsin-edta to facilitate here.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    duration_minutes: 75
    temperature_celsius: 25
    replicates: 5
control_groups:
- control_type: Technical Replicate Control
  description: Son finally senior central mean itself because throw identify indeed
    those.
- control_type: Negative Control
  description: Address view kitchen site ability return real issue opportunity child
    college newspaper take feel.
data_analysis_methods:
- One-way ANOVA with Tukey's post-hoc test
- ImageJ densitometry
