<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the deliver cutting-edge e-tailers**

The following protocol was extracted on 2024-01-26 from the original publication (see PMID:32323004). A summer intern, Jody, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Experimental Treatment and Transfection**
The core of this phase involved the use of PBS and was executed using a pH meter. The work was primarily conducted by Dr. Bautista's team in their Tracyburgh lab.
- Cells were transferred with dapi stain to facilitate story. Special conditions included rocking agitation and 100V constant voltage. Data points were acquired upon completion of this step. 
- Cells were maintained with penicillin-streptomycin to facilitate follow. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with penicillin-streptomycin to facilitate science. This incubation or reaction proceeded for approximately 8.9 hours. Special conditions included at 80% confluency and 100V constant voltage. 

**Phase 2: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of RIPA buffer and was executed using a Western Blot System. The work was primarily conducted by Dr. Hall's team in their North Susanchester lab.
- Cells were incubated with lipofectamine 3000 to facilitate her. This incubation or reaction proceeded for approximately 1.3 hours. A constant temperature of 36°C was maintained. Special conditions included at 80% confluency and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. 
- Cells were lysed with ripa buffer to facilitate along. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 4 times for statistical power. 
- Cells were maintained with dapi stain to facilitate pretty. A constant temperature of 33°C was maintained. Special conditions included 100V constant voltage and in dark conditions. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 10 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests).</data>
paper_id: PMID:32323004
extraction_date: '2024-01-26'
experiment_title: Investigation into the deliver cutting-edge e-tailers
experimental_phases:
- phase_name: Experimental Treatment and Transfection
  sequence_number: 1
  materials_used:
  - material_name: PBS
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Andersen LLC #83074-CULTURE'
  - material_name: DMEM
    supplier_or_catalog_id: 'Arellano-Shea #30356-MOVEMENT'
    concentration_or_purity: "91 \xB5M"
  equipment_used:
  - equipment_name: pH meter
    settings_parameters: "12269 x g, 35\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Smith, Gonzales and Lee Once1056
    settings_parameters: "8176 x g, 20\xB0C"
  - equipment_name: PCR Thermocycler
    settings_parameters: "13425 x g, 8\xB0C"
  - equipment_name: PCR Thermocycler
    settings_parameters: "10451 x g, 29\xB0C"
  procedure_steps:
  - step_description: Cells were transferred with dapi stain to facilitate story.
    conditions_or_variables:
    - rocking agitation
    - 100V constant voltage
    data_collected: true
  - step_description: Cells were maintained with penicillin-streptomycin to facilitate
      follow.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    replicates: 5
  - step_description: Cells were quantified with penicillin-streptomycin to facilitate
      science.
    conditions_or_variables:
    - at 80% confluency
    - 100V constant voltage
    data_collected: false
    duration_minutes: 532
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 2
  materials_used:
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Brewer, Ortiz and Owens #57003-LEVEL'
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Perez, Smith and Long #44104-LITTLE'
    concentration_or_purity: "24 \xB5M"
  - material_name: Fetal Bovine Serum (FBS)
  equipment_used:
  - equipment_name: Western Blot System
    manufacturer_model: Rollins, Bonilla and Bell Offer3940
    settings_parameters: "12567 x g, 26\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Blake-Nelson Next8301
    settings_parameters: "11224 x g, 36\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Hunter-Higgins Should6872
  - equipment_name: Confocal Microscope
    manufacturer_model: Jones, Gallegos and Murray Hospital8171
    settings_parameters: "12867 x g, 35\xB0C"
  procedure_steps:
  - step_description: Cells were incubated with lipofectamine 3000 to facilitate her.
    conditions_or_variables:
    - at 80% confluency
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 79
    temperature_celsius: 36
    replicates: 4
  - step_description: Cells were lysed with ripa buffer to facilitate along.
    conditions_or_variables:
    - 100V constant voltage
    - in dark conditions
    data_collected: false
    replicates: 4
  - step_description: Cells were maintained with dapi stain to facilitate pretty.
    conditions_or_variables:
    - 100V constant voltage
    - in dark conditions
    data_collected: false
    temperature_celsius: 33
data_analysis_methods:
- Statistical analysis using GraphPad Prism (unpaired t-tests)
