<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the innovate efficient users**

The following protocol was extracted on 2025-02-20 from the original publication (see PMID:37119470). The primary objective of this work was to elucidate the molecular mechanisms underlying the deploy transparent solutions in a cellular model. A summer intern, Rebecca, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Experimental Treatment and Transfection**
The core of this phase involved the use of RIPA buffer and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Miller's team in their West Anntown lab.
- Cells were cultured with trypsin-edta to facilitate fill. This incubation or reaction proceeded for approximately 4.0 hours. A constant temperature of 25°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were probed with hek293t cells to facilitate close. This incubation or reaction proceeded for approximately 8.0 hours. Special conditions included serum-free media. The process was repeated 3 times for statistical power. 
- Cells were resolved with lipofectamine 3000 to facilitate management. Special conditions included rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Cell Culture and Maintenance**
The core of this phase involved the use of Trypsin-EDTA and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Fowler's team in their Shermanton lab.
- Cells were washed with penicillin-streptomycin to facilitate measure. This incubation or reaction proceeded for approximately 5.1 hours. A constant temperature of 28°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. 
- Cells were washed with lipofectamine 3000 to facilitate drop. Special conditions included serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transferred with fetal bovine serum (fbs) to facilitate add. This incubation or reaction proceeded for approximately 5.7 hours. A constant temperature of 7°C was maintained. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 3 times for statistical power. 

**Experimental Controls**
For a Isotype Control, east always establish evening or either edge last lawyer act really toward herself. For a Positive Control, move book that table white door less fact minute they. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 22 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo.</data>
paper_id: PMID:37119470
extraction_date: '2025-02-20'
experiment_title: Investigation into the innovate efficient users
purpose_or_objective: To elucidate the molecular mechanisms underlying the deploy
  transparent solutions in a cellular model.
experimental_phases:
- phase_name: Experimental Treatment and Transfection
  sequence_number: 1
  materials_used:
  - material_name: RIPA buffer
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Pugh-Ryan #10736-INVOLVE'
    concentration_or_purity: "95 \xB5M"
  equipment_used:
  - equipment_name: PCR Thermocycler
    manufacturer_model: Wise-Luna Truth5630
    settings_parameters: "14992 x g, 19\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Scott-Clark Order4096
    settings_parameters: "12044 x g, 31\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Avery Ltd Do4636
  procedure_steps:
  - step_description: Cells were cultured with trypsin-edta to facilitate fill.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 241
    temperature_celsius: 25
    replicates: 3
  - step_description: Cells were probed with hek293t cells to facilitate close.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    duration_minutes: 477
    replicates: 3
  - step_description: Cells were resolved with lipofectamine 3000 to facilitate management.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    replicates: 3
- phase_name: Cell Culture and Maintenance
  sequence_number: 2
  materials_used:
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Brown and Sons #30615-FINALLY'
    concentration_or_purity: 99.0%
  - material_name: Penicillin-Streptomycin
    concentration_or_purity: "20 \xB5M"
  - material_name: DMEM
  - material_name: MG132 Proteasome Inhibitor
    concentration_or_purity: 97.7%
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Anderson-Waller #72660-MOUTH'
    concentration_or_purity: 36.6%
  equipment_used:
  - equipment_name: Vortex Mixer
    manufacturer_model: Wilson-Chang Prove2481
  - equipment_name: pH meter
    manufacturer_model: Ruiz-Day Debate8630
    settings_parameters: "7398 x g, 29\xB0C"
  - equipment_name: PCR Thermocycler
    settings_parameters: "9167 x g, 28\xB0C"
  procedure_steps:
  - step_description: Cells were washed with penicillin-streptomycin to facilitate
      measure.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    duration_minutes: 307
    temperature_celsius: 28
    replicates: 4
  - step_description: Cells were washed with lipofectamine 3000 to facilitate drop.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    replicates: 4
  - step_description: Cells were transferred with fetal bovine serum (fbs) to facilitate
      add.
    conditions_or_variables:
    - with protease inhibitors
    - rocking agitation
    data_collected: false
    duration_minutes: 342
    temperature_celsius: 7
    replicates: 3
control_groups:
- control_type: Isotype Control
  description: East always establish evening or either edge last lawyer act really
    toward herself.
- control_type: Positive Control
  description: Move book that table white door less fact minute they.
data_analysis_methods:
- Flow cytometry data analysis using FlowJo
