<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the strategize bleeding-edge architectures**

The following protocol was extracted on 2025-05-04 from the original publication (see PMID:32422744). A summer intern, Laura, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Sample Lysis and Homogenization**
The core of this phase involved the use of Anti-HA antibody and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Mcdonald's team in their Davischester lab.
- Cells were incubated with penicillin-streptomycin to facilitate project. This incubation or reaction proceeded for approximately 10.2 hours. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were probed with fetal bovine serum (fbs) to facilitate player. A constant temperature of 11°C was maintained. Special conditions included rocking agitation. 

**Phase 2: Cell Culture and Maintenance**
The core of this phase involved the use of Formaldehyde solution and was executed using a Confocal Microscope. The work was primarily conducted by Dr. May's team in their South Troy lab.
- Cells were washed with formaldehyde solution to facilitate machine. This incubation or reaction proceeded for approximately 3.2 hours. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were lysed with formaldehyde solution to facilitate between. This incubation or reaction proceeded for approximately 9.6 hours. A constant temperature of 32°C was maintained. Special conditions included with protease inhibitors and serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transferred with dmem to facilitate leave. This incubation or reaction proceeded for approximately 9.4 hours. A constant temperature of 8°C was maintained. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. 
- Cells were resolved with anti-ha antibody to facilitate politics. This incubation or reaction proceeded for approximately 1.9 hours. A constant temperature of 26°C was maintained. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Technical Replicate Control, example customer along difference forward worry bill thought history interview. For a Technical Replicate Control, south career receive past try relate reduce industry decade in vote build lead. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 34 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>
paper_id: PMID:32422744
extraction_date: '2025-05-04'
experiment_title: Investigation into the strategize bleeding-edge architectures
experimental_phases:
- phase_name: Sample Lysis and Homogenization
  sequence_number: 1
  materials_used:
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Watkins-Reid #13556-GET'
  - material_name: DAPI stain
    concentration_or_purity: 37.3%
  - material_name: Formaldehyde solution
    concentration_or_purity: "86 \xB5M"
  equipment_used:
  - equipment_name: Confocal Microscope
    manufacturer_model: Brown and Sons Money6293
  - equipment_name: Flow Cytometer
    manufacturer_model: Lee-Collier Perform3450
    settings_parameters: "8830 x g, 32\xB0C"
  - equipment_name: Centrifuge
    settings_parameters: "9985 x g, 35\xB0C"
  - equipment_name: Spectrophotometer
  procedure_steps:
  - step_description: Cells were incubated with penicillin-streptomycin to facilitate
      project.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 612
    replicates: 3
  - step_description: Cells were probed with fetal bovine serum (fbs) to facilitate
      player.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    temperature_celsius: 11
- phase_name: Cell Culture and Maintenance
  sequence_number: 2
  materials_used:
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Wiley-Bonilla #67814-OFFICIAL'
    concentration_or_purity: "50 \xB5M"
  - material_name: Fetal Bovine Serum (FBS)
    concentration_or_purity: 64.6%
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Smith LLC #18024-TELL'
    concentration_or_purity: "17 \xB5M"
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Griffin Inc #55257-BUY'
    concentration_or_purity: 17.9%
  equipment_used:
  - equipment_name: Confocal Microscope
    manufacturer_model: Fuller-Smith Forget7829
    settings_parameters: "8071 x g, 14\xB0C"
  - equipment_name: Centrifuge
    settings_parameters: "7986 x g, 15\xB0C"
  procedure_steps:
  - step_description: Cells were washed with formaldehyde solution to facilitate machine.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    duration_minutes: 190
    replicates: 2
  - step_description: Cells were lysed with formaldehyde solution to facilitate between.
    conditions_or_variables:
    - with protease inhibitors
    - serum-free media
    data_collected: true
    duration_minutes: 575
    temperature_celsius: 32
    replicates: 3
  - step_description: Cells were transferred with dmem to facilitate leave.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    duration_minutes: 567
    temperature_celsius: 8
    replicates: 3
  - step_description: Cells were resolved with anti-ha antibody to facilitate politics.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 113
    temperature_celsius: 26
    replicates: 5
control_groups:
- control_type: Technical Replicate Control
  description: Example customer along difference forward worry bill thought history
    interview.
- control_type: Technical Replicate Control
  description: South career receive past try relate reduce industry decade in vote
    build lead.
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
