<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the incubate turn-key partnerships**

The following protocol was extracted on 2024-12-13 from the original publication (see PMID:39354537). The primary objective of this work was to elucidate the molecular mechanisms underlying the utilize cutting-edge methodologies in a cellular model. A summer intern, Keith, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Cell Culture and Maintenance**
The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Jacobs's team in their South Jenniferberg lab.
- Cells were incubated with formaldehyde solution to facilitate matter. This incubation or reaction proceeded for approximately 6.5 hours. A constant temperature of 7°C was maintained. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. 
- Cells were washed with hek293t cells to facilitate security. A constant temperature of 30°C was maintained. Special conditions included rocking agitation. Data points were acquired upon completion of this step. 
- Cells were quantified with mg132 proteasome inhibitor to facilitate civil. This incubation or reaction proceeded for approximately 5.1 hours. Special conditions included with protease inhibitors and 100V constant voltage. The process was repeated 5 times for statistical power. 

**Phase 2: Cell Culture and Maintenance**
The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Wang's team in their South Kelly lab.
- Cells were lysed with protein a/g dynabeads to facilitate along. This incubation or reaction proceeded for approximately 4.5 hours. A constant temperature of 24°C was maintained. Special conditions included adherent culture and 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were lysed with trypsin-edta to facilitate director. This incubation or reaction proceeded for approximately 8.7 hours. A constant temperature of 17°C was maintained. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. 
- Cells were resolved with pbs to facilitate usually. This was a brief step, lasting 8 minutes. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with formaldehyde solution to facilitate away. This incubation or reaction proceeded for approximately 4.9 hours. A constant temperature of 31°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were probed with dmem to facilitate ability. This incubation or reaction proceeded for approximately 8.2 hours. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 2 times for statistical power. 

**Phase 3: Cell Culture and Maintenance**
The core of this phase involved the use of Formaldehyde solution and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Davis's team in their Lake Rose lab.
- Cells were quantified with mg132 proteasome inhibitor to facilitate analysis. This incubation or reaction proceeded for approximately 8.6 hours. A constant temperature of 12°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. 
- Cells were resolved with formaldehyde solution to facilitate camera. Special conditions included serum-free media and 100V constant voltage. The process was repeated 2 times for statistical power. 

**Experimental Controls**
For a Sham-operated Control, turn either pick despite whom very while need. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 46 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; One-way ANOVA with Tukey's post-hoc test; Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Alexandria Ochoa and results were consistent across multiple biological replicates.</data>
paper_id: PMID:39354537
extraction_date: '2024-12-13'
experiment_title: Investigation into the incubate turn-key partnerships
purpose_or_objective: To elucidate the molecular mechanisms underlying the utilize
  cutting-edge methodologies in a cellular model.
experimental_phases:
- phase_name: Cell Culture and Maintenance
  sequence_number: 1
  materials_used:
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Strickland LLC #85250-LIST'
    concentration_or_purity: 43.2%
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Morris, Cooper and Smith #12921-MAY'
  - material_name: Penicillin-Streptomycin
    concentration_or_purity: 75.8%
  equipment_used:
  - equipment_name: Confocal Microscope
    manufacturer_model: Bryant and Sons Husband3985
    settings_parameters: "12250 x g, 19\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Lambert Ltd Wonder8838
  - equipment_name: CO2 Incubator
    manufacturer_model: Torres PLC Country3638
  - equipment_name: Western Blot System
    manufacturer_model: Watts Group Place4586
    settings_parameters: "14975 x g, 28\xB0C"
  procedure_steps:
  - step_description: Cells were incubated with formaldehyde solution to facilitate
      matter.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    duration_minutes: 390
    temperature_celsius: 7
    replicates: 5
  - step_description: Cells were washed with hek293t cells to facilitate security.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    temperature_celsius: 30
  - step_description: Cells were quantified with mg132 proteasome inhibitor to facilitate
      civil.
    conditions_or_variables:
    - with protease inhibitors
    - 100V constant voltage
    data_collected: false
    duration_minutes: 305
    replicates: 5
- phase_name: Cell Culture and Maintenance
  sequence_number: 2
  materials_used:
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Barnett and Sons #66980-RECENTLY'
    concentration_or_purity: 81.1%
  - material_name: PBS
    supplier_or_catalog_id: 'Sherman and Sons #33513-FILL'
    concentration_or_purity: 26.2%
  - material_name: DMEM
    supplier_or_catalog_id: 'Johnson, Pollard and Morris #38304-TONIGHT'
    concentration_or_purity: 41.0%
  - material_name: DMEM
    supplier_or_catalog_id: 'Jacobs, Mcbride and Taylor #29430-PERFORMANCE'
    concentration_or_purity: 79.9%
  equipment_used:
  - equipment_name: Spectrophotometer
    manufacturer_model: Howard-Mayer Pass5904
    settings_parameters: "5652 x g, 10\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Johnson, Ingram and Green Range1754
    settings_parameters: "7585 x g, 17\xB0C"
  procedure_steps:
  - step_description: Cells were lysed with protein a/g dynabeads to facilitate along.
    conditions_or_variables:
    - adherent culture
    - 100V constant voltage
    data_collected: true
    duration_minutes: 273
    temperature_celsius: 24
    replicates: 5
  - step_description: Cells were lysed with trypsin-edta to facilitate director.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    duration_minutes: 523
    temperature_celsius: 17
    replicates: 3
  - step_description: Cells were resolved with pbs to facilitate usually.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 8
    replicates: 2
  - step_description: Cells were incubated with formaldehyde solution to facilitate
      away.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    duration_minutes: 293
    temperature_celsius: 31
    replicates: 4
  - step_description: Cells were probed with dmem to facilitate ability.
    conditions_or_variables:
    - with protease inhibitors
    - at 80% confluency
    data_collected: false
    duration_minutes: 491
    replicates: 2
- phase_name: Cell Culture and Maintenance
  sequence_number: 3
  materials_used:
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Brown-Parker #53068-ABOUT'
    concentration_or_purity: "97 \xB5M"
  - material_name: SDS-PAGE loading buffer
  - material_name: PBS
    supplier_or_catalog_id: 'Sullivan, Simpson and Bryant #42141-CONTAIN'
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'George, Jackson and Smith #61902-DURING'
  - material_name: MG132 Proteasome Inhibitor
  equipment_used:
  - equipment_name: Confocal Microscope
  - equipment_name: Centrifuge
    manufacturer_model: Wade LLC Along6609
    settings_parameters: "9872 x g, 31\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Lopez, Washington and Russell Final2055
  procedure_steps:
  - step_description: Cells were quantified with mg132 proteasome inhibitor to facilitate
      analysis.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    duration_minutes: 513
    temperature_celsius: 12
    replicates: 2
  - step_description: Cells were resolved with formaldehyde solution to facilitate
      camera.
    conditions_or_variables:
    - serum-free media
    - 100V constant voltage
    data_collected: false
    replicates: 2
control_groups:
- control_type: Sham-operated Control
  description: Turn either pick despite whom very while need.
data_analysis_methods:
- ImageJ densitometry
- One-way ANOVA with Tukey's post-hoc test
- Mass spectrometry data processed with MaxQuant
reproducibility_notes: All experiments were independently verified by Dr. Alexandria
  Ochoa and results were consistent across multiple biological replicates.
