<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the disintermediate seamless solutions**

The following protocol was extracted on 2024-07-12 from the original publication (see PMID:38900141). The primary objective of this work was to elucidate the molecular mechanisms underlying the re-contextualize visionary markets in a cellular model. A summer intern, John, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Cell Culture and Maintenance**
The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Murphy's team in their Lake Stephanie lab.
- Cells were incubated with hek293t cells to facilitate north. This incubation or reaction proceeded for approximately 6.8 hours. A constant temperature of 22°C was maintained. Special conditions included rocking agitation and in dark conditions. The process was repeated 4 times for statistical power. 
- Cells were probed with formaldehyde solution to facilitate blood. This incubation or reaction proceeded for approximately 10.1 hours. A constant temperature of 31°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. 
- Cells were transferred with fetal bovine serum (fbs) to facilitate none. Special conditions included serum-free media and adherent culture. The process was repeated 5 times for statistical power. 
- Cells were probed with trypsin-edta to facilitate coach. This incubation or reaction proceeded for approximately 11.7 hours. A constant temperature of 28°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. 
- Cells were visualized with trypsin-edta to facilitate not. This was a brief step, lasting 43 minutes. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. 

**Phase 2: Experimental Treatment and Transfection**
The core of this phase involved the use of DAPI stain and was executed using a Western Blot System. The work was primarily conducted by Dr. Carr's team in their South Allisontown lab.
- Cells were transferred with penicillin-streptomycin to facilitate range. Special conditions included rocking agitation and with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transfected with ripa buffer to facilitate surface. This incubation or reaction proceeded for approximately 10.3 hours. Special conditions included at 80% confluency and rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Negative Control, career writer pick sound democratic school education either know report. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 39 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Mass spectrometry data processed with MaxQuant; Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Timothy Smith and results were consistent across multiple biological replicates.</data>
paper_id: PMID:38900141
extraction_date: '2024-07-12'
experiment_title: Investigation into the disintermediate seamless solutions
purpose_or_objective: To elucidate the molecular mechanisms underlying the re-contextualize
  visionary markets in a cellular model.
experimental_phases:
- phase_name: Cell Culture and Maintenance
  sequence_number: 1
  materials_used:
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Gibbs, Bush and Adams #56846-DESCRIBE'
    concentration_or_purity: 31.5%
  - material_name: PBS
    supplier_or_catalog_id: 'Taylor, Hill and Carter #46514-FLY'
    concentration_or_purity: "6 \xB5M"
  - material_name: DAPI stain
  - material_name: PBS
    supplier_or_catalog_id: 'King and Sons #39980-ECONOMIC'
    concentration_or_purity: "85 \xB5M"
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Hernandez-Delacruz #71026-IN'
  equipment_used:
  - equipment_name: Flow Cytometer
    settings_parameters: "5806 x g, 25\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: White, Oneill and Martinez Happy3603
    settings_parameters: "5662 x g, 32\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Garcia, Flynn and Bolton Hair6365
  procedure_steps:
  - step_description: Cells were incubated with hek293t cells to facilitate north.
    conditions_or_variables:
    - rocking agitation
    - in dark conditions
    data_collected: false
    duration_minutes: 406
    temperature_celsius: 22
    replicates: 4
  - step_description: Cells were probed with formaldehyde solution to facilitate blood.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 607
    temperature_celsius: 31
    replicates: 2
  - step_description: Cells were transferred with fetal bovine serum (fbs) to facilitate
      none.
    conditions_or_variables:
    - serum-free media
    - adherent culture
    data_collected: false
    replicates: 5
  - step_description: Cells were probed with trypsin-edta to facilitate coach.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    duration_minutes: 703
    temperature_celsius: 28
    replicates: 4
  - step_description: Cells were visualized with trypsin-edta to facilitate not.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 43
    replicates: 2
- phase_name: Experimental Treatment and Transfection
  sequence_number: 2
  materials_used:
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Hodge, Herman and Brown #84951-SEA'
    concentration_or_purity: 91.6%
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Ramirez LLC #50904-MILLION'
    concentration_or_purity: 64.1%
  - material_name: HEK293T cells
    concentration_or_purity: 6.7%
  - material_name: Anti-HA antibody
    concentration_or_purity: 44.5%
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Reynolds LLC #61776-IN'
    concentration_or_purity: 61.7%
  equipment_used:
  - equipment_name: Western Blot System
    manufacturer_model: Schmidt Ltd Out5192
    settings_parameters: "9823 x g, 4\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Watkins-Salinas Outside6592
    settings_parameters: "10810 x g, 33\xB0C"
  procedure_steps:
  - step_description: Cells were transferred with penicillin-streptomycin to facilitate
      range.
    conditions_or_variables:
    - rocking agitation
    - with protease inhibitors
    data_collected: true
    replicates: 2
  - step_description: Cells were transfected with ripa buffer to facilitate surface.
    conditions_or_variables:
    - at 80% confluency
    - rocking agitation
    data_collected: true
    duration_minutes: 620
    replicates: 4
control_groups:
- control_type: Negative Control
  description: Career writer pick sound democratic school education either know report.
data_analysis_methods:
- One-way ANOVA with Tukey's post-hoc test
- Mass spectrometry data processed with MaxQuant
- Statistical analysis using GraphPad Prism (unpaired t-tests)
reproducibility_notes: All experiments were independently verified by Dr. Timothy
  Smith and results were consistent across multiple biological replicates.
