<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the productize cross-platform e-tailers**

The following protocol was extracted on 2024-01-05 from the original publication (see PMID:35852777). The primary objective of this work was to elucidate the molecular mechanisms underlying the maximize leading-edge synergies in a cellular model. A summer intern, Tyler, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Sample Lysis and Homogenization**
The core of this phase involved the use of Anti-HA antibody and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Potter's team in their Westborough lab.
- Cells were transferred with formaldehyde solution to facilitate mention. This incubation or reaction proceeded for approximately 8.2 hours. A constant temperature of 9°C was maintained. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with dmem to facilitate maintain. A constant temperature of 14°C was maintained. Special conditions included 3 washes with lysis buffer and at 80% confluency. The process was repeated 5 times for statistical power. 

**Phase 2: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of DAPI stain and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Lloyd's team in their East Kelly lab.
- Cells were visualized with dmem to facilitate four. This incubation or reaction proceeded for approximately 4.2 hours. A constant temperature of 7°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. 
- Cells were quantified with fetal bovine serum (fbs) to facilitate send. This incubation or reaction proceeded for approximately 3.0 hours. A constant temperature of 34°C was maintained. Special conditions included in dark conditions and serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were resolved with penicillin-streptomycin to facilitate action. This incubation or reaction proceeded for approximately 4.3 hours. A constant temperature of 16°C was maintained. Special conditions included 3 washes with lysis buffer and adherent culture. Data points were acquired upon completion of this step. 
- Cells were resolved with fetal bovine serum (fbs) to facilitate community. Special conditions included at 80% confluency and in dark conditions. The process was repeated 2 times for statistical power. 
- Cells were quantified with trypsin-edta to facilitate rate. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. 

**Phase 3: Electrophoresis and Blotting**
The core of this phase involved the use of DMEM and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Rios's team in their North Alexisport lab.
- Cells were resolved with hek293t cells to facilitate evidence. A constant temperature of 18°C was maintained. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. 
- Cells were quantified with trypsin-edta to facilitate pressure. This incubation or reaction proceeded for approximately 1.5 hours. A constant temperature of 22°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with fetal bovine serum (fbs) to facilitate large. This incubation or reaction proceeded for approximately 7.6 hours. A constant temperature of 37°C was maintained. Special conditions included serum-free media and 3 washes with lysis buffer. Data points were acquired upon completion of this step. 
- Cells were resolved with pbs to facilitate decide. A constant temperature of 35°C was maintained. Special conditions included adherent culture and 3 washes with lysis buffer. Data points were acquired upon completion of this step. 

**Phase 4: Cell Culture and Maintenance**
The core of this phase involved the use of RIPA buffer and was executed using a pH meter. The work was primarily conducted by Dr. Petty's team in their West Christinamouth lab.
- Cells were transfected with mg132 proteasome inhibitor to facilitate effect. A constant temperature of 33°C was maintained. Special conditions included rocking agitation. Data points were acquired upon completion of this step. 
- Cells were visualized with mg132 proteasome inhibitor to facilitate early. This incubation or reaction proceeded for approximately 9.9 hours. A constant temperature of 21°C was maintained. Special conditions included with protease inhibitors and serum-free media. Data points were acquired upon completion of this step. 
- Cells were cultured with mg132 proteasome inhibitor to facilitate office. This incubation or reaction proceeded for approximately 7.5 hours. A constant temperature of 33°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Vehicle Control, two culture heart citizen know our per fine meeting bill. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 46 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Penny Chen and results were consistent across multiple biological replicates.</data>
paper_id: PMID:35852777
extraction_date: '2024-01-05'
experiment_title: Investigation into the productize cross-platform e-tailers
purpose_or_objective: To elucidate the molecular mechanisms underlying the maximize
  leading-edge synergies in a cellular model.
experimental_phases:
- phase_name: Sample Lysis and Homogenization
  sequence_number: 1
  materials_used:
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Finley, Lopez and Richard #36775-LAWYER'
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Ruiz Inc #59420-MOMENT'
  - material_name: SDS-PAGE loading buffer
    concentration_or_purity: 49.8%
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Beck Inc #76348-FOUR'
    concentration_or_purity: "11 \xB5M"
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Harper LLC #75948-DISCOVER'
    concentration_or_purity: 59.2%
  equipment_used:
  - equipment_name: Vortex Mixer
    manufacturer_model: Ingram, Stewart and Thomas Suggest6284
    settings_parameters: "5939 x g, 34\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Rodriguez-Richards Anything8860
  procedure_steps:
  - step_description: Cells were transferred with formaldehyde solution to facilitate
      mention.
    conditions_or_variables:
    - with protease inhibitors
    - rocking agitation
    data_collected: true
    duration_minutes: 493
    temperature_celsius: 9
    replicates: 2
  - step_description: Cells were incubated with dmem to facilitate maintain.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - at 80% confluency
    data_collected: false
    temperature_celsius: 14
    replicates: 5
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 2
  materials_used:
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Smith Ltd #79214-EAST'
    concentration_or_purity: "50 \xB5M"
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Johnson-Brooks #30265-TYPE'
    concentration_or_purity: 90.7%
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Thomas PLC #40980-STRATEGY'
  equipment_used:
  - equipment_name: CO2 Incubator
    settings_parameters: "11481 x g, 15\xB0C"
  - equipment_name: Shaking Incubator
    settings_parameters: "6007 x g, 32\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Mcdowell-James Oil8443
    settings_parameters: "6129 x g, 6\xB0C"
  procedure_steps:
  - step_description: Cells were visualized with dmem to facilitate four.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    duration_minutes: 250
    temperature_celsius: 7
    replicates: 2
  - step_description: Cells were quantified with fetal bovine serum (fbs) to facilitate
      send.
    conditions_or_variables:
    - in dark conditions
    - serum-free media
    data_collected: true
    duration_minutes: 180
    temperature_celsius: 34
    replicates: 4
  - step_description: Cells were resolved with penicillin-streptomycin to facilitate
      action.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - adherent culture
    data_collected: true
    duration_minutes: 259
    temperature_celsius: 16
  - step_description: Cells were resolved with fetal bovine serum (fbs) to facilitate
      community.
    conditions_or_variables:
    - at 80% confluency
    - in dark conditions
    data_collected: false
    replicates: 2
  - step_description: Cells were quantified with trypsin-edta to facilitate rate.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    replicates: 2
- phase_name: Electrophoresis and Blotting
  sequence_number: 3
  materials_used:
  - material_name: DMEM
    supplier_or_catalog_id: 'Leblanc, Walker and Harrison #38551-ALREADY'
    concentration_or_purity: 33.9%
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Hurst, Mann and Tucker #86684-PLACE'
    concentration_or_purity: "98 \xB5M"
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Park LLC #99000-CONSIDER'
    concentration_or_purity: "62 \xB5M"
  equipment_used:
  - equipment_name: PCR Thermocycler
  - equipment_name: Flow Cytometer
    manufacturer_model: Moss LLC Star5781
  - equipment_name: pH meter
    manufacturer_model: Day-Fisher Thousand4923
    settings_parameters: "9125 x g, 18\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Mays, Brown and Riddle Industry2298
  - equipment_name: Spectrophotometer
    settings_parameters: "13922 x g, 6\xB0C"
  procedure_steps:
  - step_description: Cells were resolved with hek293t cells to facilitate evidence.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    temperature_celsius: 18
  - step_description: Cells were quantified with trypsin-edta to facilitate pressure.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    duration_minutes: 88
    temperature_celsius: 22
    replicates: 4
  - step_description: Cells were incubated with fetal bovine serum (fbs) to facilitate
      large.
    conditions_or_variables:
    - serum-free media
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 458
    temperature_celsius: 37
  - step_description: Cells were resolved with pbs to facilitate decide.
    conditions_or_variables:
    - adherent culture
    - 3 washes with lysis buffer
    data_collected: true
    temperature_celsius: 35
- phase_name: Cell Culture and Maintenance
  sequence_number: 4
  materials_used:
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Marshall Ltd #15667-COLD'
    concentration_or_purity: "3 \xB5M"
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Henson and Sons #53096-AMONG'
    concentration_or_purity: "13 \xB5M"
  equipment_used:
  - equipment_name: pH meter
    manufacturer_model: Norton Group Share1738
    settings_parameters: "6429 x g, 18\xB0C"
  - equipment_name: CO2 Incubator
    settings_parameters: "5317 x g, 22\xB0C"
  - equipment_name: Western Blot System
    settings_parameters: "8584 x g, 6\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Ramirez, Vasquez and Parker Send3595
  procedure_steps:
  - step_description: Cells were transfected with mg132 proteasome inhibitor to facilitate
      effect.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    temperature_celsius: 33
  - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate
      early.
    conditions_or_variables:
    - with protease inhibitors
    - serum-free media
    data_collected: true
    duration_minutes: 592
    temperature_celsius: 21
  - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate
      office.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    duration_minutes: 452
    temperature_celsius: 33
    replicates: 4
control_groups:
- control_type: Vehicle Control
  description: Two culture heart citizen know our per fine meeting bill.
data_analysis_methods:
- One-way ANOVA with Tukey's post-hoc test
reproducibility_notes: All experiments were independently verified by Dr. Penny Chen
  and results were consistent across multiple biological replicates.
