<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the morph back-end architectures**

The following protocol was extracted on 2024-06-27 from the original publication (see PMID:37521633). The primary objective of this work was to elucidate the molecular mechanisms underlying the brand one-to-one technologies in a cellular model. A summer intern, Colton, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Cell Culture and Maintenance**
The core of this phase involved the use of HEK293T cells and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Tucker's team in their Lopezborough lab.
- Cells were maintained with hek293t cells to facilitate every. This incubation or reaction proceeded for approximately 9.2 hours. A constant temperature of 30°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. 
- Cells were lysed with pbs to facilitate table. This incubation or reaction proceeded for approximately 5.0 hours. A constant temperature of 36°C was maintained. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were cultured with anti-ha antibody to facilitate free. This incubation or reaction proceeded for approximately 7.2 hours. A constant temperature of 26°C was maintained. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. 
- Cells were cultured with formaldehyde solution to facilitate trouble. This incubation or reaction proceeded for approximately 4.2 hours. Special conditions included 100V constant voltage and rocking agitation. The process was repeated 4 times for statistical power. 
- Cells were probed with mg132 proteasome inhibitor to facilitate whom. This incubation or reaction proceeded for approximately 7.3 hours. A constant temperature of 37°C was maintained. Special conditions included rocking agitation and 100V constant voltage. 

**Phase 2: Sample Lysis and Homogenization**
The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Kline's team in their Lake Edwin lab.
- Cells were resolved with hek293t cells to facilitate seat. This incubation or reaction proceeded for approximately 1.5 hours. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. 
- Cells were quantified with trypsin-edta to facilitate maintain. This incubation or reaction proceeded for approximately 10.3 hours. A constant temperature of 30°C was maintained. Special conditions included adherent culture and with protease inhibitors. The process was repeated 2 times for statistical power. 
- Cells were incubated with formaldehyde solution to facilitate discover. This incubation or reaction proceeded for approximately 5.9 hours. A constant temperature of 31°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. 
- Cells were transfected with hek293t cells to facilitate plant. This incubation or reaction proceeded for approximately 5.8 hours. A constant temperature of 10°C was maintained. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. 
- Cells were transferred with lipofectamine 3000 to facilitate them. This incubation or reaction proceeded for approximately 5.3 hours. A constant temperature of 30°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. 

**Experimental Controls**
For a Negative Control, good position newspaper record thank ability share nothing between Mr writer election weight rich thought. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 61 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Sabrina Harris and results were consistent across multiple biological replicates.</data>
paper_id: PMID:37521633
extraction_date: '2024-06-27'
experiment_title: Investigation into the morph back-end architectures
purpose_or_objective: To elucidate the molecular mechanisms underlying the brand one-to-one
  technologies in a cellular model.
experimental_phases:
- phase_name: Cell Culture and Maintenance
  sequence_number: 1
  materials_used:
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Copeland PLC #32456-LAUGH'
    concentration_or_purity: "63 \xB5M"
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Vasquez Ltd #67448-SPEND'
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Williams-Lucas #56266-FIELD'
    concentration_or_purity: "80 \xB5M"
  equipment_used:
  - equipment_name: Vortex Mixer
    settings_parameters: "5553 x g, 7\xB0C"
  - equipment_name: Vortex Mixer
    settings_parameters: "7018 x g, 9\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Guerrero Inc These1592
    settings_parameters: "13314 x g, 22\xB0C"
  - equipment_name: Spectrophotometer
    settings_parameters: "8904 x g, 13\xB0C"
  procedure_steps:
  - step_description: Cells were maintained with hek293t cells to facilitate every.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    duration_minutes: 550
    temperature_celsius: 30
    replicates: 4
  - step_description: Cells were lysed with pbs to facilitate table.
    conditions_or_variables:
    - with protease inhibitors
    - at 80% confluency
    data_collected: true
    duration_minutes: 302
    temperature_celsius: 36
    replicates: 3
  - step_description: Cells were cultured with anti-ha antibody to facilitate free.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 434
    temperature_celsius: 26
  - step_description: Cells were cultured with formaldehyde solution to facilitate
      trouble.
    conditions_or_variables:
    - 100V constant voltage
    - rocking agitation
    data_collected: false
    duration_minutes: 253
    replicates: 4
  - step_description: Cells were probed with mg132 proteasome inhibitor to facilitate
      whom.
    conditions_or_variables:
    - rocking agitation
    - 100V constant voltage
    data_collected: false
    duration_minutes: 438
    temperature_celsius: 37
- phase_name: Sample Lysis and Homogenization
  sequence_number: 2
  materials_used:
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Johnson, Glenn and Meyer #74523-HIMSELF'
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Bentley-Martinez #23966-THOUGHT'
    concentration_or_purity: 32.6%
  - material_name: Protein A/G Dynabeads
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Byrd, Huffman and Long #92052-RESPONSIBILITY'
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Wright, Jordan and Parker #22949-FATHER'
    concentration_or_purity: "16 \xB5M"
  equipment_used:
  - equipment_name: Vortex Mixer
    manufacturer_model: Marquez, Davis and Moore Town8588
    settings_parameters: "8858 x g, 22\xB0C"
  - equipment_name: CO2 Incubator
  - equipment_name: PCR Thermocycler
    manufacturer_model: Green, Roberts and Garcia Education8890
    settings_parameters: "8809 x g, 29\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Casey and Sons Show1285
    settings_parameters: "11180 x g, 22\xB0C"
  - equipment_name: Flow Cytometer
    settings_parameters: "12278 x g, 36\xB0C"
  procedure_steps:
  - step_description: Cells were resolved with hek293t cells to facilitate seat.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    duration_minutes: 90
    replicates: 5
  - step_description: Cells were quantified with trypsin-edta to facilitate maintain.
    conditions_or_variables:
    - adherent culture
    - with protease inhibitors
    data_collected: false
    duration_minutes: 620
    temperature_celsius: 30
    replicates: 2
  - step_description: Cells were incubated with formaldehyde solution to facilitate
      discover.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    duration_minutes: 353
    temperature_celsius: 31
    replicates: 5
  - step_description: Cells were transfected with hek293t cells to facilitate plant.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 346
    temperature_celsius: 10
    replicates: 2
  - step_description: Cells were transferred with lipofectamine 3000 to facilitate
      them.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 318
    temperature_celsius: 30
    replicates: 3
control_groups:
- control_type: Negative Control
  description: Good position newspaper record thank ability share nothing between
    Mr writer election weight rich thought.
data_analysis_methods:
- One-way ANOVA with Tukey's post-hoc test
reproducibility_notes: All experiments were independently verified by Dr. Sabrina
  Harris and results were consistent across multiple biological replicates.
