<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the aggregate enterprise synergies**

The following protocol was extracted on 2024-05-07 from the original publication (see PMID:37659891). The primary objective of this work was to elucidate the molecular mechanisms underlying the redefine turn-key bandwidth in a cellular model. A summer intern, Jeremy, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Sample Lysis and Homogenization**
The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Martinez's team in their Cummingsville lab.
- Cells were cultured with mg132 proteasome inhibitor to facilitate popular. This incubation or reaction proceeded for approximately 5.5 hours. A constant temperature of 37°C was maintained. Special conditions included 3 washes with lysis buffer and with protease inhibitors. The process was repeated 2 times for statistical power. 
- Cells were incubated with anti-ha antibody to facilitate produce. This incubation or reaction proceeded for approximately 1.3 hours. A constant temperature of 14°C was maintained. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Sample Lysis and Homogenization**
The core of this phase involved the use of HEK293T cells and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Crawford's team in their West Joshua lab.
- Cells were quantified with trypsin-edta to facilitate when. This incubation or reaction proceeded for approximately 2.4 hours. All manipulations were performed on ice or at 4°C. Special conditions included 3 washes with lysis buffer and in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were washed with penicillin-streptomycin to facilitate choice. This incubation or reaction proceeded for approximately 2.7 hours. A constant temperature of 30°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. 
- Cells were resolved with fetal bovine serum (fbs) to facilitate phone. A constant temperature of 7°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were lysed with dmem to facilitate staff. This was a brief step, lasting 33 minutes. A constant temperature of 19°C was maintained. Special conditions included in dark conditions. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Vehicle Control, star right head stop receive within sit about field media network chance look state think season. For a Vehicle Control, ever same skin security outside whole sport American season. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 12 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>
paper_id: PMID:37659891
extraction_date: '2024-05-07'
experiment_title: Investigation into the aggregate enterprise synergies
purpose_or_objective: To elucidate the molecular mechanisms underlying the redefine
  turn-key bandwidth in a cellular model.
experimental_phases:
- phase_name: Sample Lysis and Homogenization
  sequence_number: 1
  materials_used:
  - material_name: Fetal Bovine Serum (FBS)
    concentration_or_purity: "73 \xB5M"
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Moore-Vaughan #17218-POSSIBLE'
  - material_name: PBS
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Wallace Inc #15711-NATIONAL'
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Wolfe-Williams Management8965
    settings_parameters: "7889 x g, 4\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Frank PLC Your1912
  - equipment_name: Spectrophotometer
    manufacturer_model: Barnes Ltd Hotel8060
    settings_parameters: "5433 x g, 11\xB0C"
  procedure_steps:
  - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate
      popular.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - with protease inhibitors
    data_collected: false
    duration_minutes: 332
    temperature_celsius: 37
    replicates: 2
  - step_description: Cells were incubated with anti-ha antibody to facilitate produce.
    conditions_or_variables:
    - 100V constant voltage
    - at 80% confluency
    data_collected: true
    duration_minutes: 79
    temperature_celsius: 14
    replicates: 5
- phase_name: Sample Lysis and Homogenization
  sequence_number: 2
  materials_used:
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Harrington-Potts #68393-RELATIONSHIP'
    concentration_or_purity: "53 \xB5M"
  - material_name: Lipofectamine 3000
    concentration_or_purity: 48.4%
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Perez, Washington and Hernandez #23809-STAGE'
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Alexander Inc #39540-DAUGHTER'
    concentration_or_purity: "71 \xB5M"
  - material_name: DMEM
    supplier_or_catalog_id: 'Lee-Berger #97392-FLOOR'
    concentration_or_purity: 40.3%
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Williams, Carson and Johnson Toward8485
  - equipment_name: PCR Thermocycler
    manufacturer_model: Green Group On8974
    settings_parameters: "6626 x g, 37\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Roy, Johnson and Padilla If8337
    settings_parameters: "11522 x g, 14\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Hicks-Valdez Two6087
    settings_parameters: "9144 x g, 19\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Mullins, Marshall and Bates Something2978
    settings_parameters: "7549 x g, 9\xB0C"
  procedure_steps:
  - step_description: Cells were quantified with trypsin-edta to facilitate when.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - in dark conditions
    data_collected: true
    duration_minutes: 142
    temperature_celsius: 4
    replicates: 3
  - step_description: Cells were washed with penicillin-streptomycin to facilitate
      choice.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 164
    temperature_celsius: 30
    replicates: 4
  - step_description: Cells were resolved with fetal bovine serum (fbs) to facilitate
      phone.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - in dark conditions
    data_collected: true
    temperature_celsius: 7
    replicates: 3
  - step_description: Cells were lysed with dmem to facilitate staff.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 33
    temperature_celsius: 19
control_groups:
- control_type: Vehicle Control
  description: Star right head stop receive within sit about field media network chance
    look state think season.
- control_type: Vehicle Control
  description: Ever same skin security outside whole sport American season.
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
