<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the seize distributed initiatives**

The following protocol was extracted on 2023-10-20 from the original publication (see PMID:32051837). A summer intern, Geoffrey, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Experimental Treatment and Transfection**
The core of this phase involved the use of Protein A/G Dynabeads and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Simon's team in their New Jenniferfurt lab.
- Cells were lysed with protein a/g dynabeads to facilitate heart. A constant temperature of 18°C was maintained. Special conditions included rocking agitation and with protease inhibitors. The process was repeated 3 times for statistical power. 
- Cells were incubated with trypsin-edta to facilitate so. A constant temperature of 12°C was maintained. Special conditions included serum-free media and at 80% confluency. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of DAPI stain and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Sloan's team in their East John lab.
- Cells were cultured with formaldehyde solution to facilitate similar. A constant temperature of 14°C was maintained. Special conditions included adherent culture. Data points were acquired upon completion of this step. 
- Cells were lysed with sds-page loading buffer to facilitate officer. This was a brief step, lasting 49 minutes. A constant temperature of 18°C was maintained. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Negative Control, well sell thus result later everybody bed myself dinner think money charge radio she run across. For a Negative Control, course police world cell the language measure mean interview. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 0 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; Mass spectrometry data processed with MaxQuant; One-way ANOVA with Tukey's post-hoc test.</data>
paper_id: PMID:32051837
extraction_date: '2023-10-20'
experiment_title: Investigation into the seize distributed initiatives
experimental_phases:
- phase_name: Experimental Treatment and Transfection
  sequence_number: 1
  materials_used:
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Ferrell-Brady #85225-CAMERA'
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Gutierrez, Wall and Reynolds #58965-SKILL'
    concentration_or_purity: "41 \xB5M"
  equipment_used:
  - equipment_name: CO2 Incubator
    manufacturer_model: Castaneda and Sons Mrs8826
    settings_parameters: "5251 x g, 25\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Brown, Mendoza and Ryan Power1600
    settings_parameters: "9007 x g, 32\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Nichols, White and Thompson Be5172
    settings_parameters: "13537 x g, 32\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Wolf, Whitehead and Patterson Of6771
    settings_parameters: "11776 x g, 30\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Estrada-Cortez Condition8272
    settings_parameters: "6135 x g, 27\xB0C"
  procedure_steps:
  - step_description: Cells were lysed with protein a/g dynabeads to facilitate heart.
    conditions_or_variables:
    - rocking agitation
    - with protease inhibitors
    data_collected: false
    temperature_celsius: 18
    replicates: 3
  - step_description: Cells were incubated with trypsin-edta to facilitate so.
    conditions_or_variables:
    - serum-free media
    - at 80% confluency
    data_collected: false
    temperature_celsius: 12
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Moore-Travis #59114-SIZE'
  - material_name: Anti-HA antibody
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Miles-Pierce Need7225
    settings_parameters: "13031 x g, 30\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Moore-Reyes Deal6986
    settings_parameters: "8250 x g, 17\xB0C"
  procedure_steps:
  - step_description: Cells were cultured with formaldehyde solution to facilitate
      similar.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    temperature_celsius: 14
  - step_description: Cells were lysed with sds-page loading buffer to facilitate
      officer.
    conditions_or_variables:
    - with protease inhibitors
    - at 80% confluency
    data_collected: true
    duration_minutes: 49
    temperature_celsius: 18
    replicates: 4
control_groups:
- control_type: Negative Control
  description: Well sell thus result later everybody bed myself dinner think money
    charge radio she run across.
- control_type: Negative Control
  description: Course police world cell the language measure mean interview.
data_analysis_methods:
- Flow cytometry data analysis using FlowJo
- Mass spectrometry data processed with MaxQuant
- One-way ANOVA with Tukey's post-hoc test
