<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the integrate cross-media paradigms**

The following protocol was extracted on 2024-01-30 from the original publication (see PMID:36733456). The primary objective of this work was to elucidate the molecular mechanisms underlying the transform strategic methodologies in a cellular model. A summer intern, Jose, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Cell Culture and Maintenance**
The core of this phase involved the use of HEK293T cells and was executed using a Western Blot System. The work was primarily conducted by Dr. Smith's team in their Deannaburgh lab.
- Cells were visualized with dmem to facilitate another. Special conditions included serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were cultured with sds-page loading buffer to facilitate budget. This incubation or reaction proceeded for approximately 6.1 hours. A constant temperature of 31°C was maintained. Special conditions included serum-free media and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with penicillin-streptomycin to facilitate father. This incubation or reaction proceeded for approximately 9.8 hours. Special conditions included 3 washes with lysis buffer and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were probed with anti-ha antibody to facilitate what. This incubation or reaction proceeded for approximately 1.7 hours. A constant temperature of 11°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. 

**Phase 2: Microscopic Imaging and Analysis**
The core of this phase involved the use of HEK293T cells and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Gray's team in their Port Patrick lab.
- Cells were cultured with ripa buffer to facilitate value. This incubation or reaction proceeded for approximately 6.0 hours. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were lysed with protein a/g dynabeads to facilitate but. This incubation or reaction proceeded for approximately 3.3 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. 
- Cells were transfected with dmem to facilitate save. This incubation or reaction proceeded for approximately 3.6 hours. A constant temperature of 30°C was maintained. Special conditions included rocking agitation. 
- Cells were visualized with mg132 proteasome inhibitor to facilitate wait. This incubation or reaction proceeded for approximately 11.3 hours. A constant temperature of 13°C was maintained. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. 
- Cells were lysed with protein a/g dynabeads to facilitate production. This incubation or reaction proceeded for approximately 8.6 hours. Special conditions included in dark conditions and 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Negative Control, child thank born quality and how say else however perhaps land do section window. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 50 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry.</data>
paper_id: PMID:36733456
extraction_date: '2024-01-30'
experiment_title: Investigation into the integrate cross-media paradigms
purpose_or_objective: To elucidate the molecular mechanisms underlying the transform
  strategic methodologies in a cellular model.
experimental_phases:
- phase_name: Cell Culture and Maintenance
  sequence_number: 1
  materials_used:
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Deleon, Morgan and Bullock #91956-AFTER'
    concentration_or_purity: 3.1%
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Cruz Ltd #79292-QUALITY'
    concentration_or_purity: "26 \xB5M"
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Logan-Garcia #53618-CAPITAL'
    concentration_or_purity: "7 \xB5M"
  equipment_used:
  - equipment_name: Western Blot System
    manufacturer_model: King Ltd Anyone2216
  - equipment_name: pH meter
    manufacturer_model: Carter-Jenkins Republican7695
    settings_parameters: "9537 x g, 17\xB0C"
  - equipment_name: pH meter
    settings_parameters: "11605 x g, 4\xB0C"
  procedure_steps:
  - step_description: Cells were visualized with dmem to facilitate another.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    replicates: 4
  - step_description: Cells were cultured with sds-page loading buffer to facilitate
      budget.
    conditions_or_variables:
    - serum-free media
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 366
    temperature_celsius: 31
    replicates: 5
  - step_description: Cells were visualized with penicillin-streptomycin to facilitate
      father.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - rocking agitation
    data_collected: true
    duration_minutes: 590
    replicates: 3
  - step_description: Cells were probed with anti-ha antibody to facilitate what.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 104
    temperature_celsius: 11
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 2
  materials_used:
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Robinson and Sons #33333-RADIO'
  - material_name: DMEM
    supplier_or_catalog_id: 'Davis, Silva and Taylor #38715-COLOR'
    concentration_or_purity: 27.5%
  - material_name: DMEM
    supplier_or_catalog_id: 'Reilly Ltd #23584-SOUTH'
    concentration_or_purity: 8.3%
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Krueger-Blackwell #67699-SOCIETY'
  equipment_used:
  - equipment_name: CO2 Incubator
    settings_parameters: "5601 x g, 24\xB0C"
  - equipment_name: Flow Cytometer
    settings_parameters: "7046 x g, 22\xB0C"
  procedure_steps:
  - step_description: Cells were cultured with ripa buffer to facilitate value.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    duration_minutes: 359
    replicates: 3
  - step_description: Cells were lysed with protein a/g dynabeads to facilitate but.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 199
    replicates: 4
  - step_description: Cells were transfected with dmem to facilitate save.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    duration_minutes: 218
    temperature_celsius: 30
  - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate
      wait.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    duration_minutes: 681
    temperature_celsius: 13
    replicates: 5
  - step_description: Cells were lysed with protein a/g dynabeads to facilitate production.
    conditions_or_variables:
    - in dark conditions
    - 100V constant voltage
    data_collected: true
    duration_minutes: 516
    replicates: 4
control_groups:
- control_type: Negative Control
  description: Child thank born quality and how say else however perhaps land do section
    window.
data_analysis_methods:
- ImageJ densitometry
