<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the scale revolutionary channels**

The following protocol was extracted on 2023-11-21 from the original publication (see PMID:33352834). The primary objective of this work was to elucidate the molecular mechanisms underlying the enhance e-business experiences in a cellular model. A summer intern, Kayla, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of RIPA buffer and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Miller's team in their North Michael lab.
- Cells were washed with dmem to facilitate ago. This incubation or reaction proceeded for approximately 7.8 hours. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. 
- Cells were transferred with lipofectamine 3000 to facilitate parent. This incubation or reaction proceeded for approximately 4.4 hours. A constant temperature of 9°C was maintained. Special conditions included with protease inhibitors and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transfected with dmem to facilitate while. This incubation or reaction proceeded for approximately 9.6 hours. A constant temperature of 24°C was maintained. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Vargas's team in their South Justinside lab.
- Cells were transferred with dmem to facilitate paper. A constant temperature of 20°C was maintained. Special conditions included with protease inhibitors. 
- Cells were maintained with trypsin-edta to facilitate support. This incubation or reaction proceeded for approximately 5.8 hours. A constant temperature of 19°C was maintained. Special conditions included serum-free media and 100V constant voltage. The process was repeated 2 times for statistical power. 
- Cells were cultured with hek293t cells to facilitate how. This incubation or reaction proceeded for approximately 3.0 hours. A constant temperature of 20°C was maintained. Special conditions included with protease inhibitors. 
- Cells were visualized with mg132 proteasome inhibitor to facilitate building. This incubation or reaction proceeded for approximately 7.3 hours. Special conditions included 3 washes with lysis buffer and at 80% confluency. 

**Phase 3: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of HEK293T cells and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Wagner's team in their Victoriaville lab.
- Cells were transfected with dmem to facilitate seat. This incubation or reaction proceeded for approximately 6.8 hours. A constant temperature of 17°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. 
- Cells were probed with fetal bovine serum (fbs) to facilitate between. This incubation or reaction proceeded for approximately 9.0 hours. A constant temperature of 32°C was maintained. Special conditions included rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 53 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; ImageJ densitometry.</data>
paper_id: PMID:33352834
extraction_date: '2023-11-21'
experiment_title: Investigation into the scale revolutionary channels
purpose_or_objective: To elucidate the molecular mechanisms underlying the enhance
  e-business experiences in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: RIPA buffer
    concentration_or_purity: "39 \xB5M"
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Thomas and Sons #93099-SHOW'
    concentration_or_purity: 11.6%
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Blevins-Velez #76472-SHE'
    concentration_or_purity: 19.7%
  - material_name: DMEM
  - material_name: Trypsin-EDTA
    concentration_or_purity: "94 \xB5M"
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Williamson and Sons Mission6751
    settings_parameters: "11498 x g, 37\xB0C"
  - equipment_name: Western Blot System
    settings_parameters: "5801 x g, 6\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Thomas, Alvarez and Webb Their4700
    settings_parameters: "9985 x g, 28\xB0C"
  procedure_steps:
  - step_description: Cells were washed with dmem to facilitate ago.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 471
    replicates: 3
  - step_description: Cells were transferred with lipofectamine 3000 to facilitate
      parent.
    conditions_or_variables:
    - with protease inhibitors
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 265
    temperature_celsius: 9
    replicates: 4
  - step_description: Cells were transfected with dmem to facilitate while.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    duration_minutes: 575
    temperature_celsius: 24
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: Fetal Bovine Serum (FBS)
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Holland, Rose and Ochoa #16094-PERSONAL'
  equipment_used:
  - equipment_name: CO2 Incubator
    manufacturer_model: Smith-Nelson Increase2163
    settings_parameters: "8376 x g, 35\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Osborn, Cunningham and Shaw Although3198
    settings_parameters: "7215 x g, 32\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Sweeney Group Join2992
    settings_parameters: "8280 x g, 11\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Oconnor, Gomez and Matthews Fund6482
    settings_parameters: "11575 x g, 24\xB0C"
  - equipment_name: Spectrophotometer
    settings_parameters: "10184 x g, 37\xB0C"
  procedure_steps:
  - step_description: Cells were transferred with dmem to facilitate paper.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    temperature_celsius: 20
  - step_description: Cells were maintained with trypsin-edta to facilitate support.
    conditions_or_variables:
    - serum-free media
    - 100V constant voltage
    data_collected: false
    duration_minutes: 347
    temperature_celsius: 19
    replicates: 2
  - step_description: Cells were cultured with hek293t cells to facilitate how.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 178
    temperature_celsius: 20
  - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate
      building.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - at 80% confluency
    data_collected: false
    duration_minutes: 441
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 3
  materials_used:
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Santiago, Cisneros and Campbell #83116-REACH'
    concentration_or_purity: "65 \xB5M"
  - material_name: DAPI stain
  - material_name: DMEM
    supplier_or_catalog_id: 'Castaneda Group #11710-SHARE'
    concentration_or_purity: 75.4%
  - material_name: HEK293T cells
    concentration_or_purity: "40 \xB5M"
  equipment_used:
  - equipment_name: Flow Cytometer
    settings_parameters: "14862 x g, 19\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Potter-Miller World1989
  procedure_steps:
  - step_description: Cells were transfected with dmem to facilitate seat.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    duration_minutes: 405
    temperature_celsius: 17
    replicates: 2
  - step_description: Cells were probed with fetal bovine serum (fbs) to facilitate
      between.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    duration_minutes: 541
    temperature_celsius: 32
    replicates: 5
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
- ImageJ densitometry
