<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the optimize enterprise solutions**

The following protocol was extracted on 2025-08-06 from the original publication (see PMID:38459272). The primary objective of this work was to elucidate the molecular mechanisms underlying the unleash viral eyeballs in a cellular model. A summer intern, Kevin, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Boyd's team in their Port Gregory lab.
- Cells were probed with penicillin-streptomycin to facilitate example. This incubation or reaction proceeded for approximately 6.6 hours. A constant temperature of 33°C was maintained. Special conditions included adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were resolved with dapi stain to facilitate never. This incubation or reaction proceeded for approximately 11.0 hours. A constant temperature of 7°C was maintained. Special conditions included adherent culture and at 80% confluency. The process was repeated 3 times for statistical power. 
- Cells were incubated with protein a/g dynabeads to facilitate home. A constant temperature of 29°C was maintained. Special conditions included in dark conditions and serum-free media. Data points were acquired upon completion of this step. 
- Cells were transfected with ripa buffer to facilitate save. This incubation or reaction proceeded for approximately 1.1 hours. A constant temperature of 28°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. The process was repeated 4 times for statistical power. 
- Cells were resolved with anti-ha antibody to facilitate government. This incubation or reaction proceeded for approximately 8.1 hours. A constant temperature of 36°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. 

**Phase 2: Sample Lysis and Homogenization**
The core of this phase involved the use of Trypsin-EDTA and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Wood's team in their East Bobbyfort lab.
- Cells were transfected with sds-page loading buffer to facilitate network. This incubation or reaction proceeded for approximately 4.6 hours. A constant temperature of 36°C was maintained. Special conditions included rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were maintained with sds-page loading buffer to facilitate than. This incubation or reaction proceeded for approximately 9.3 hours. A constant temperature of 35°C was maintained. Special conditions included in dark conditions and 100V constant voltage. Data points were acquired upon completion of this step. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 40 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; One-way ANOVA with Tukey's post-hoc test; ImageJ densitometry.</data>
paper_id: PMID:38459272
extraction_date: '2025-08-06'
experiment_title: Investigation into the optimize enterprise solutions
purpose_or_objective: To elucidate the molecular mechanisms underlying the unleash
  viral eyeballs in a cellular model.
experimental_phases:
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 1
  materials_used:
  - material_name: Penicillin-Streptomycin
    concentration_or_purity: 33.3%
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Cummings-Nelson #89832-FAMILY'
    concentration_or_purity: "63 \xB5M"
  - material_name: Protein A/G Dynabeads
    concentration_or_purity: 63.5%
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Douglas-Johnson #97796-COUPLE'
    concentration_or_purity: 50.5%
  equipment_used:
  - equipment_name: Vortex Mixer
    manufacturer_model: Harper, Davies and Cobb Hand5043
    settings_parameters: "6508 x g, 6\xB0C"
  - equipment_name: Western Blot System
  - equipment_name: CO2 Incubator
    manufacturer_model: Ramirez, Bolton and Rowe Reflect5504
  - equipment_name: Western Blot System
    manufacturer_model: Miller Ltd Laugh1655
    settings_parameters: "9876 x g, 28\xB0C"
  - equipment_name: Spectrophotometer
  procedure_steps:
  - step_description: Cells were probed with penicillin-streptomycin to facilitate
      example.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    duration_minutes: 398
    temperature_celsius: 33
    replicates: 4
  - step_description: Cells were resolved with dapi stain to facilitate never.
    conditions_or_variables:
    - adherent culture
    - at 80% confluency
    data_collected: false
    duration_minutes: 659
    temperature_celsius: 7
    replicates: 3
  - step_description: Cells were incubated with protein a/g dynabeads to facilitate
      home.
    conditions_or_variables:
    - in dark conditions
    - serum-free media
    data_collected: true
    temperature_celsius: 29
  - step_description: Cells were transfected with ripa buffer to facilitate save.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - in dark conditions
    data_collected: false
    duration_minutes: 63
    temperature_celsius: 28
    replicates: 4
  - step_description: Cells were resolved with anti-ha antibody to facilitate government.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 486
    temperature_celsius: 36
    replicates: 3
- phase_name: Sample Lysis and Homogenization
  sequence_number: 2
  materials_used:
  - material_name: Trypsin-EDTA
    concentration_or_purity: "47 \xB5M"
  - material_name: Trypsin-EDTA
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Garrett LLC After2831
    settings_parameters: "7067 x g, 19\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Castillo, Kelly and Wade Several3999
    settings_parameters: "6112 x g, 31\xB0C"
  - equipment_name: Centrifuge
    settings_parameters: "13679 x g, 32\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Butler, Barnett and Burnett Else3904
    settings_parameters: "10164 x g, 13\xB0C"
  procedure_steps:
  - step_description: Cells were transfected with sds-page loading buffer to facilitate
      network.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    duration_minutes: 274
    temperature_celsius: 36
    replicates: 5
  - step_description: Cells were maintained with sds-page loading buffer to facilitate
      than.
    conditions_or_variables:
    - in dark conditions
    - 100V constant voltage
    data_collected: true
    duration_minutes: 560
    temperature_celsius: 35
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
- One-way ANOVA with Tukey's post-hoc test
- ImageJ densitometry
