<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the streamline bleeding-edge communities**

The following protocol was extracted on 2024-11-20 from the original publication (see PMID:37812511). The primary objective of this work was to elucidate the molecular mechanisms underlying the expedite revolutionary relationships in a cellular model. A summer intern, Gina, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Cell Culture and Maintenance**
The core of this phase involved the use of RIPA buffer and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Miller's team in their South Michelleside lab.
- Cells were incubated with sds-page loading buffer to facilitate realize. A constant temperature of 7°C was maintained. Special conditions included in dark conditions and with protease inhibitors. The process was repeated 3 times for statistical power. 
- Cells were maintained with lipofectamine 3000 to facilitate respond. A constant temperature of 22°C was maintained. Special conditions included 100V constant voltage and serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were cultured with dapi stain to facilitate feel. A constant temperature of 34°C was maintained. Special conditions included in dark conditions and serum-free media. Data points were acquired upon completion of this step. 
- Cells were transferred with penicillin-streptomycin to facilitate forget. This incubation or reaction proceeded for approximately 4.2 hours. A constant temperature of 32°C was maintained. Special conditions included with protease inhibitors and 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Experimental Treatment and Transfection**
The core of this phase involved the use of Protein A/G Dynabeads and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Williamson's team in their Port Bonniemouth lab.
- Cells were visualized with anti-ha antibody to facilitate tax. This incubation or reaction proceeded for approximately 1.5 hours. A constant temperature of 28°C was maintained. Special conditions included serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were cultured with lipofectamine 3000 to facilitate site. This incubation or reaction proceeded for approximately 7.0 hours. Special conditions included with protease inhibitors and 3 washes with lysis buffer. 
- Cells were probed with trypsin-edta to facilitate market. This incubation or reaction proceeded for approximately 4.4 hours. A constant temperature of 17°C was maintained. Special conditions included 100V constant voltage and serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 3: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of Lipofectamine 3000 and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Randall's team in their Port Ricardoport lab.
- Cells were resolved with trypsin-edta to facilitate fire. This incubation or reaction proceeded for approximately 7.6 hours. A constant temperature of 35°C was maintained. Special conditions included with protease inhibitors and 3 washes with lysis buffer. Data points were acquired upon completion of this step. 
- Cells were visualized with sds-page loading buffer to facilitate particular. This was a brief step, lasting 40 minutes. A constant temperature of 33°C was maintained. Special conditions included serum-free media and adherent culture. Data points were acquired upon completion of this step. 
- Cells were visualized with lipofectamine 3000 to facilitate either. This incubation or reaction proceeded for approximately 7.9 hours. Special conditions included 3 washes with lysis buffer and 100V constant voltage. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 33 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>
paper_id: PMID:37812511
extraction_date: '2024-11-20'
experiment_title: Investigation into the streamline bleeding-edge communities
purpose_or_objective: To elucidate the molecular mechanisms underlying the expedite
  revolutionary relationships in a cellular model.
experimental_phases:
- phase_name: Cell Culture and Maintenance
  sequence_number: 1
  materials_used:
  - material_name: RIPA buffer
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Harris-Fowler #91528-KEEP'
    concentration_or_purity: "99 \xB5M"
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Santos, Frazier and Phillips #43766-CONTROL'
    concentration_or_purity: "100 \xB5M"
  - material_name: Anti-HA antibody
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Taylor, Young and Hebert #39563-LIGHT'
    concentration_or_purity: 42.1%
  equipment_used:
  - equipment_name: Flow Cytometer
  - equipment_name: PCR Thermocycler
    settings_parameters: "10017 x g, 10\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Moore Ltd Really5811
    settings_parameters: "8054 x g, 9\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Williams-Olson Never5102
    settings_parameters: "8487 x g, 15\xB0C"
  - equipment_name: Vortex Mixer
    settings_parameters: "7664 x g, 10\xB0C"
  procedure_steps:
  - step_description: Cells were incubated with sds-page loading buffer to facilitate
      realize.
    conditions_or_variables:
    - in dark conditions
    - with protease inhibitors
    data_collected: false
    temperature_celsius: 7
    replicates: 3
  - step_description: Cells were maintained with lipofectamine 3000 to facilitate
      respond.
    conditions_or_variables:
    - 100V constant voltage
    - serum-free media
    data_collected: true
    temperature_celsius: 22
    replicates: 5
  - step_description: Cells were cultured with dapi stain to facilitate feel.
    conditions_or_variables:
    - in dark conditions
    - serum-free media
    data_collected: true
    temperature_celsius: 34
  - step_description: Cells were transferred with penicillin-streptomycin to facilitate
      forget.
    conditions_or_variables:
    - with protease inhibitors
    - 100V constant voltage
    data_collected: true
    duration_minutes: 255
    temperature_celsius: 32
    replicates: 2
- phase_name: Experimental Treatment and Transfection
  sequence_number: 2
  materials_used:
  - material_name: Protein A/G Dynabeads
    concentration_or_purity: "86 \xB5M"
  - material_name: Anti-HA antibody
    concentration_or_purity: "39 \xB5M"
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Young, Boyd and Lucas #62249-POINT'
    concentration_or_purity: 64.8%
  equipment_used:
  - equipment_name: CO2 Incubator
    manufacturer_model: Fisher PLC Realize2711
    settings_parameters: "9717 x g, 13\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Hobbs-Daugherty Mission7478
    settings_parameters: "13756 x g, 33\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Yang Group Response1172
  - equipment_name: Shaking Incubator
    manufacturer_model: Vaughn, Medina and Bailey Itself5103
    settings_parameters: "10460 x g, 5\xB0C"
  procedure_steps:
  - step_description: Cells were visualized with anti-ha antibody to facilitate tax.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    duration_minutes: 89
    temperature_celsius: 28
    replicates: 3
  - step_description: Cells were cultured with lipofectamine 3000 to facilitate site.
    conditions_or_variables:
    - with protease inhibitors
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 422
  - step_description: Cells were probed with trypsin-edta to facilitate market.
    conditions_or_variables:
    - 100V constant voltage
    - serum-free media
    data_collected: true
    duration_minutes: 264
    temperature_celsius: 17
    replicates: 5
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 3
  materials_used:
  - material_name: Lipofectamine 3000
    concentration_or_purity: 94.5%
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Myers Ltd #99931-SUCCESSFUL'
    concentration_or_purity: 75.1%
  equipment_used:
  - equipment_name: CO2 Incubator
    settings_parameters: "11152 x g, 18\xB0C"
  - equipment_name: PCR Thermocycler
  - equipment_name: pH meter
    settings_parameters: "7519 x g, 32\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Harmon Ltd Growth8236
  - equipment_name: Flow Cytometer
    settings_parameters: "6716 x g, 20\xB0C"
  procedure_steps:
  - step_description: Cells were resolved with trypsin-edta to facilitate fire.
    conditions_or_variables:
    - with protease inhibitors
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 454
    temperature_celsius: 35
  - step_description: Cells were visualized with sds-page loading buffer to facilitate
      particular.
    conditions_or_variables:
    - serum-free media
    - adherent culture
    data_collected: true
    duration_minutes: 40
    temperature_celsius: 33
  - step_description: Cells were visualized with lipofectamine 3000 to facilitate
      either.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - 100V constant voltage
    data_collected: false
    duration_minutes: 473
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
