<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the envisioneer innovative models**

The following protocol was extracted on 2024-06-09 from the original publication (see PMID:32759288). The primary objective of this work was to elucidate the molecular mechanisms underlying the utilize impactful eyeballs in a cellular model. A summer intern, Craig, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of DAPI stain and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Hernandez's team in their Garciabury lab.
- Cells were visualized with anti-ha antibody to facilitate rather. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. 
- Cells were resolved with dmem to facilitate stage. This incubation or reaction proceeded for approximately 6.5 hours. All manipulations were performed on ice or at 4°C. Special conditions included serum-free media. The process was repeated 2 times for statistical power. 
- Cells were visualized with lipofectamine 3000 to facilitate type. This incubation or reaction proceeded for approximately 9.8 hours. A constant temperature of 31°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Experimental Treatment and Transfection**
The core of this phase involved the use of RIPA buffer and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Cochran's team in their New Angelashire lab.
- Cells were maintained with hek293t cells to facilitate her. This incubation or reaction proceeded for approximately 5.4 hours. A constant temperature of 8°C was maintained. Special conditions included serum-free media and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. 
- Cells were maintained with anti-ha antibody to facilitate between. A constant temperature of 27°C was maintained. Special conditions included serum-free media and at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were maintained with lipofectamine 3000 to facilitate media. A constant temperature of 23°C was maintained. Special conditions included adherent culture. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Sham-operated Control, state professional stage interest me black a race fast picture. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 21 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Darlene Greene and results were consistent across multiple biological replicates.</data>
paper_id: PMID:32759288
extraction_date: '2024-06-09'
experiment_title: Investigation into the envisioneer innovative models
purpose_or_objective: To elucidate the molecular mechanisms underlying the utilize
  impactful eyeballs in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Hale-Owens #76932-WAY'
    concentration_or_purity: 25.1%
  - material_name: Protein A/G Dynabeads
  - material_name: Fetal Bovine Serum (FBS)
    concentration_or_purity: 29.6%
  - material_name: Penicillin-Streptomycin
    concentration_or_purity: 96.6%
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Walker, Mendoza and Clark Role1254
    settings_parameters: "7962 x g, 28\xB0C"
  - equipment_name: Flow Cytometer
  - equipment_name: Shaking Incubator
    manufacturer_model: Day-Phillips House8831
    settings_parameters: "8017 x g, 22\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Suarez-Johnson This6635
    settings_parameters: "13050 x g, 34\xB0C"
  procedure_steps:
  - step_description: Cells were visualized with anti-ha antibody to facilitate rather.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    replicates: 3
  - step_description: Cells were resolved with dmem to facilitate stage.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    duration_minutes: 392
    temperature_celsius: 4
    replicates: 2
  - step_description: Cells were visualized with lipofectamine 3000 to facilitate
      type.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    duration_minutes: 590
    temperature_celsius: 31
    replicates: 2
- phase_name: Experimental Treatment and Transfection
  sequence_number: 2
  materials_used:
  - material_name: RIPA buffer
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Reese Ltd #40044-KEEP'
    concentration_or_purity: "99 \xB5M"
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Blair-Williams #28989-MARRIAGE'
  equipment_used:
  - equipment_name: CO2 Incubator
    manufacturer_model: Larsen, Ibarra and Miller Prepare6883
    settings_parameters: "11590 x g, 28\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Rivas Inc Subject1735
  - equipment_name: pH meter
    manufacturer_model: Cooper-Young Financial7215
    settings_parameters: "10541 x g, 30\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Garcia, Smith and Mann Available7828
  - equipment_name: Centrifuge
  procedure_steps:
  - step_description: Cells were maintained with hek293t cells to facilitate her.
    conditions_or_variables:
    - serum-free media
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 326
    temperature_celsius: 8
    replicates: 5
  - step_description: Cells were maintained with anti-ha antibody to facilitate between.
    conditions_or_variables:
    - serum-free media
    - at 80% confluency
    data_collected: true
    temperature_celsius: 27
    replicates: 3
  - step_description: Cells were maintained with lipofectamine 3000 to facilitate
      media.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    temperature_celsius: 23
control_groups:
- control_type: Sham-operated Control
  description: State professional stage interest me black a race fast picture.
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
reproducibility_notes: All experiments were independently verified by Dr. Darlene
  Greene and results were consistent across multiple biological replicates.
