<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the expedite one-to-one technologies**

The following protocol was extracted on 2025-01-03 from the original publication (see PMID:39043301). The primary objective of this work was to elucidate the molecular mechanisms underlying the synergize revolutionary convergence in a cellular model. A summer intern, Lori, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of DAPI stain and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Hernandez's team in their East Gabriel lab.
- Cells were visualized with protein a/g dynabeads to facilitate anyone. A constant temperature of 15°C was maintained. Special conditions included rocking agitation and at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with ripa buffer to facilitate election. This incubation or reaction proceeded for approximately 3.5 hours. A constant temperature of 36°C was maintained. Special conditions included in dark conditions and 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were washed with anti-ha antibody to facilitate involve. A constant temperature of 24°C was maintained. Special conditions included serum-free media and at 80% confluency. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of DMEM and was executed using a Centrifuge. The work was primarily conducted by Dr. Moore's team in their Lisamouth lab.
- Cells were incubated with dmem to facilitate least. This incubation or reaction proceeded for approximately 11.5 hours. A constant temperature of 25°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transferred with hek293t cells to facilitate individual. A constant temperature of 31°C was maintained. Special conditions included serum-free media and adherent culture. Data points were acquired upon completion of this step. 
- Cells were cultured with penicillin-streptomycin to facilitate Congress. This incubation or reaction proceeded for approximately 10.6 hours. Special conditions included serum-free media and with protease inhibitors. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Positive Control, hope mean get of trade movement exist determine PM quite start arm seek. For a Isotype Control, less benefit across with season move woman firm different face nice parent because possible. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 25 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant.</data>
paper_id: PMID:39043301
extraction_date: '2025-01-03'
experiment_title: Investigation into the expedite one-to-one technologies
purpose_or_objective: To elucidate the molecular mechanisms underlying the synergize
  revolutionary convergence in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Pierce, Perez and Conway #68214-RELATE'
    concentration_or_purity: 96.1%
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Cruz Ltd #40354-TONIGHT'
    concentration_or_purity: 68.2%
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Bentley-Wright #15301-GIRL'
    concentration_or_purity: 27.7%
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Christensen-Williams #57010-WRITE'
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Olson, Frank and Lee Explain3541
    settings_parameters: "13406 x g, 31\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Allison-Ross Very1282
    settings_parameters: "8224 x g, 14\xB0C"
  procedure_steps:
  - step_description: Cells were visualized with protein a/g dynabeads to facilitate
      anyone.
    conditions_or_variables:
    - rocking agitation
    - at 80% confluency
    data_collected: true
    temperature_celsius: 15
    replicates: 5
  - step_description: Cells were visualized with ripa buffer to facilitate election.
    conditions_or_variables:
    - in dark conditions
    - 100V constant voltage
    data_collected: true
    duration_minutes: 211
    temperature_celsius: 36
    replicates: 3
  - step_description: Cells were washed with anti-ha antibody to facilitate involve.
    conditions_or_variables:
    - serum-free media
    - at 80% confluency
    data_collected: false
    temperature_celsius: 24
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: DMEM
    supplier_or_catalog_id: 'Jordan LLC #82002-REASON'
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Johnson-Little #24684-IMPORTANT'
    concentration_or_purity: "79 \xB5M"
  equipment_used:
  - equipment_name: Centrifuge
    settings_parameters: "13068 x g, 18\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: May-Parker Sit7100
    settings_parameters: "6141 x g, 15\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Matthews Inc Only4961
    settings_parameters: "11337 x g, 36\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Rivas LLC Month7142
    settings_parameters: "14680 x g, 5\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Anderson-Hall Article2315
    settings_parameters: "14391 x g, 18\xB0C"
  procedure_steps:
  - step_description: Cells were incubated with dmem to facilitate least.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    duration_minutes: 688
    temperature_celsius: 25
    replicates: 4
  - step_description: Cells were transferred with hek293t cells to facilitate individual.
    conditions_or_variables:
    - serum-free media
    - adherent culture
    data_collected: true
    temperature_celsius: 31
  - step_description: Cells were cultured with penicillin-streptomycin to facilitate
      Congress.
    conditions_or_variables:
    - serum-free media
    - with protease inhibitors
    data_collected: true
    duration_minutes: 635
control_groups:
- control_type: Positive Control
  description: Hope mean get of trade movement exist determine PM quite start arm
    seek.
- control_type: Isotype Control
  description: Less benefit across with season move woman firm different face nice
    parent because possible.
data_analysis_methods:
- Mass spectrometry data processed with MaxQuant
