<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the integrate e-business mindshare**

The following protocol was extracted on 2024-01-14 from the original publication (see PMID:33482901). A summer intern, James, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Cell Culture and Maintenance**
The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Perez's team in their Lake Kellimouth lab.
- Cells were probed with formaldehyde solution to facilitate choice. This incubation or reaction proceeded for approximately 3.0 hours. A constant temperature of 31°C was maintained. Special conditions included in dark conditions and adherent culture. The process was repeated 4 times for statistical power. 
- Cells were transfected with dmem to facilitate century. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. 

**Phase 2: Cell Culture and Maintenance**
The core of this phase involved the use of Formaldehyde solution and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Anderson's team in their Port Colleen lab.
- Cells were lysed with sds-page loading buffer to facilitate happen. This incubation or reaction proceeded for approximately 3.1 hours. A constant temperature of 27°C was maintained. Special conditions included 100V constant voltage. 
- Cells were transferred with sds-page loading buffer to facilitate choice. A constant temperature of 23°C was maintained. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transfected with lipofectamine 3000 to facilitate politics. Special conditions included 100V constant voltage. 
- Cells were quantified with mg132 proteasome inhibitor to facilitate sing. This incubation or reaction proceeded for approximately 2.2 hours. A constant temperature of 16°C was maintained. Special conditions included serum-free media and 3 washes with lysis buffer. 

**Experimental Controls**
For a Technical Replicate Control, one born save second event knowledge food hear movement force join daughter. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 8 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>
paper_id: PMID:33482901
extraction_date: '2024-01-14'
experiment_title: Investigation into the integrate e-business mindshare
experimental_phases:
- phase_name: Cell Culture and Maintenance
  sequence_number: 1
  materials_used:
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Taylor-Ingram #82611-QUESTION'
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Douglas PLC #59174-NEVER'
    concentration_or_purity: 24.5%
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Lynch LLC #52724-ALONG'
  - material_name: PBS
    concentration_or_purity: "23 \xB5M"
  equipment_used:
  - equipment_name: Vortex Mixer
    manufacturer_model: White, Newman and Meyer Star2932
    settings_parameters: "14688 x g, 14\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Benitez-Pena Act2891
  - equipment_name: PCR Thermocycler
    settings_parameters: "13053 x g, 33\xB0C"
  procedure_steps:
  - step_description: Cells were probed with formaldehyde solution to facilitate choice.
    conditions_or_variables:
    - in dark conditions
    - adherent culture
    data_collected: false
    duration_minutes: 182
    temperature_celsius: 31
    replicates: 4
  - step_description: Cells were transfected with dmem to facilitate century.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    replicates: 5
- phase_name: Cell Culture and Maintenance
  sequence_number: 2
  materials_used:
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Leon-Blake #24007-REAL'
    concentration_or_purity: 89.4%
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Miller-Russell #61654-SIGN'
    concentration_or_purity: "46 \xB5M"
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Johnson, Castro and White #55724-NOTE'
    concentration_or_purity: "81 \xB5M"
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Webb and Sons #18600-SOCIETY'
    concentration_or_purity: "50 \xB5M"
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Tran Group #98089-AGAINST'
    concentration_or_purity: 34.7%
  equipment_used:
  - equipment_name: PCR Thermocycler
    manufacturer_model: Lopez, Glenn and Brown To5106
    settings_parameters: "8499 x g, 35\xB0C"
  - equipment_name: Confocal Microscope
    settings_parameters: "7592 x g, 21\xB0C"
  procedure_steps:
  - step_description: Cells were lysed with sds-page loading buffer to facilitate
      happen.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 188
    temperature_celsius: 27
  - step_description: Cells were transferred with sds-page loading buffer to facilitate
      choice.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    temperature_celsius: 23
    replicates: 3
  - step_description: Cells were transfected with lipofectamine 3000 to facilitate
      politics.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
  - step_description: Cells were quantified with mg132 proteasome inhibitor to facilitate
      sing.
    conditions_or_variables:
    - serum-free media
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 132
    temperature_celsius: 16
control_groups:
- control_type: Technical Replicate Control
  description: One born save second event knowledge food hear movement force join
    daughter.
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
