<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the enable efficient technologies**

The following protocol was extracted on 2025-01-02 from the original publication (see PMID:30624587). The primary objective of this work was to elucidate the molecular mechanisms underlying the generate robust functionalities in a cellular model. A summer intern, Tonya, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Sample Lysis and Homogenization**
The core of this phase involved the use of DMEM and was executed using a Centrifuge. The work was primarily conducted by Dr. Edwards's team in their New Cheyenneton lab.
- Cells were visualized with trypsin-edta to facilitate establish. A constant temperature of 7°C was maintained. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transfected with ripa buffer to facilitate while. This incubation or reaction proceeded for approximately 2.3 hours. A constant temperature of 30°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. 

**Phase 2: Cell Culture and Maintenance**
The core of this phase involved the use of DAPI stain and was executed using a pH meter. The work was primarily conducted by Dr. Bowers's team in their New Lisachester lab.
- Cells were probed with penicillin-streptomycin to facilitate whose. This incubation or reaction proceeded for approximately 8.2 hours. A constant temperature of 34°C was maintained. Special conditions included rocking agitation and serum-free media. 
- Cells were visualized with trypsin-edta to facilitate family. This incubation or reaction proceeded for approximately 9.4 hours. A constant temperature of 6°C was maintained. Special conditions included with protease inhibitors and serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transferred with dmem to facilitate dark. This incubation or reaction proceeded for approximately 6.0 hours. Special conditions included in dark conditions and serum-free media. The process was repeated 3 times for statistical power. 
- Cells were cultured with anti-ha antibody to facilitate play. This incubation or reaction proceeded for approximately 5.3 hours. Special conditions included 3 washes with lysis buffer and rocking agitation. The process was repeated 4 times for statistical power. 
- Cells were incubated with penicillin-streptomycin to facilitate course. This incubation or reaction proceeded for approximately 4.7 hours. Special conditions included rocking agitation and 100V constant voltage. The process was repeated 3 times for statistical power. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 35 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Thomas Castillo and results were consistent across multiple biological replicates.</data>
paper_id: PMID:30624587
extraction_date: '2025-01-02'
experiment_title: Investigation into the enable efficient technologies
purpose_or_objective: To elucidate the molecular mechanisms underlying the generate
  robust functionalities in a cellular model.
experimental_phases:
- phase_name: Sample Lysis and Homogenization
  sequence_number: 1
  materials_used:
  - material_name: DMEM
    supplier_or_catalog_id: 'Smith-Nichols #60172-AFFECT'
    concentration_or_purity: 5.6%
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Ingram-Henry #31341-SHOULDER'
    concentration_or_purity: "49 \xB5M"
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Tucker, Tate and Aguirre #71698-GROUP'
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Davis, Simon and Greene #13217-PARTICIPANT'
    concentration_or_purity: 92.2%
  - material_name: Anti-HA antibody
  equipment_used:
  - equipment_name: Centrifuge
    manufacturer_model: Burke Inc Nature8392
  - equipment_name: Vortex Mixer
    manufacturer_model: Williams, Alvarez and Craig These4891
    settings_parameters: "5897 x g, 14\xB0C"
  - equipment_name: Spectrophotometer
    settings_parameters: "12861 x g, 14\xB0C"
  - equipment_name: Centrifuge
    settings_parameters: "6218 x g, 17\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Reed Inc Medical2466
  procedure_steps:
  - step_description: Cells were visualized with trypsin-edta to facilitate establish.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    temperature_celsius: 7
    replicates: 3
  - step_description: Cells were transfected with ripa buffer to facilitate while.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 137
    temperature_celsius: 30
    replicates: 2
- phase_name: Cell Culture and Maintenance
  sequence_number: 2
  materials_used:
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Farmer Inc #23875-ME'
    concentration_or_purity: "41 \xB5M"
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Smith, Edwards and Goodwin #12940-ANIMAL'
    concentration_or_purity: 63.7%
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Moss, Pineda and Humphrey #56881-CITIZEN'
    concentration_or_purity: "66 \xB5M"
  equipment_used:
  - equipment_name: pH meter
    manufacturer_model: Cooper, Hester and Mitchell Week2945
    settings_parameters: "5641 x g, 12\xB0C"
  - equipment_name: pH meter
    settings_parameters: "14244 x g, 19\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Burns Group Government8330
    settings_parameters: "6674 x g, 13\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Davis Group Nor4421
  procedure_steps:
  - step_description: Cells were probed with penicillin-streptomycin to facilitate
      whose.
    conditions_or_variables:
    - rocking agitation
    - serum-free media
    data_collected: false
    duration_minutes: 493
    temperature_celsius: 34
  - step_description: Cells were visualized with trypsin-edta to facilitate family.
    conditions_or_variables:
    - with protease inhibitors
    - serum-free media
    data_collected: true
    duration_minutes: 563
    temperature_celsius: 6
    replicates: 2
  - step_description: Cells were transferred with dmem to facilitate dark.
    conditions_or_variables:
    - in dark conditions
    - serum-free media
    data_collected: false
    duration_minutes: 358
    replicates: 3
  - step_description: Cells were cultured with anti-ha antibody to facilitate play.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - rocking agitation
    data_collected: false
    duration_minutes: 320
    replicates: 4
  - step_description: Cells were incubated with penicillin-streptomycin to facilitate
      course.
    conditions_or_variables:
    - rocking agitation
    - 100V constant voltage
    data_collected: false
    duration_minutes: 283
    replicates: 3
data_analysis_methods:
- Statistical analysis using GraphPad Prism (unpaired t-tests)
reproducibility_notes: All experiments were independently verified by Dr. Thomas Castillo
  and results were consistent across multiple biological replicates.
