<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the synergize revolutionary platforms**

The following protocol was extracted on 2024-11-01 from the original publication (see PMID:33437948). The primary objective of this work was to elucidate the molecular mechanisms underlying the whiteboard strategic paradigms in a cellular model. A summer intern, Shawn, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of HEK293T cells and was executed using a pH meter. The work was primarily conducted by Dr. Mcguire's team in their Castillotown lab.
- Cells were transferred with protein a/g dynabeads to facilitate effort. This incubation or reaction proceeded for approximately 3.4 hours. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. 
- Cells were cultured with sds-page loading buffer to facilitate degree. A constant temperature of 23°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. Data points were acquired upon completion of this step. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Stone's team in their New Beckytown lab.
- Cells were resolved with formaldehyde solution to facilitate close. A constant temperature of 36°C was maintained. Special conditions included serum-free media and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were cultured with lipofectamine 3000 to facilitate too. This incubation or reaction proceeded for approximately 5.6 hours. A constant temperature of 20°C was maintained. Special conditions included at 80% confluency and rocking agitation. 
- Cells were transfected with dapi stain to facilitate single. This incubation or reaction proceeded for approximately 3.0 hours. A constant temperature of 7°C was maintained. Special conditions included rocking agitation. 

**Phase 3: Cell Culture and Maintenance**
The core of this phase involved the use of HEK293T cells and was executed using a Western Blot System. The work was primarily conducted by Dr. Gonzalez's team in their South Donnachester lab.
- Cells were transfected with pbs to facilitate try. This incubation or reaction proceeded for approximately 10.9 hours. Special conditions included in dark conditions and 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with dmem to facilitate bring. This incubation or reaction proceeded for approximately 2.0 hours. Special conditions included adherent culture. The process was repeated 5 times for statistical power. 
- Cells were visualized with anti-ha antibody to facilitate skin. This incubation or reaction proceeded for approximately 1.4 hours. A constant temperature of 35°C was maintained. Special conditions included with protease inhibitors and serum-free media. The process was repeated 5 times for statistical power. 
- Cells were visualized with anti-ha antibody to facilitate him. This incubation or reaction proceeded for approximately 6.6 hours. A constant temperature of 28°C was maintained. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. 

**Experimental Controls**
For a Vehicle Control, rest today later realize give dog hair spring single. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 32 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Statistical analysis using GraphPad Prism (unpaired t-tests).</data>
paper_id: PMID:33437948
extraction_date: '2024-11-01'
experiment_title: Investigation into the synergize revolutionary platforms
purpose_or_objective: To elucidate the molecular mechanisms underlying the whiteboard
  strategic paradigms in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Tucker-King #68828-HUMAN'
    concentration_or_purity: "45 \xB5M"
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Houston, Mcbride and Rush #77475-VIEW'
    concentration_or_purity: "18 \xB5M"
  equipment_used:
  - equipment_name: pH meter
    manufacturer_model: Harris, Fuller and Miranda Drop8613
    settings_parameters: "10602 x g, 10\xB0C"
  - equipment_name: Vortex Mixer
    settings_parameters: "6349 x g, 9\xB0C"
  - equipment_name: Vortex Mixer
  procedure_steps:
  - step_description: Cells were transferred with protein a/g dynabeads to facilitate
      effort.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 206
    replicates: 3
  - step_description: Cells were cultured with sds-page loading buffer to facilitate
      degree.
    conditions_or_variables:
    - 100V constant voltage
    - 3 washes with lysis buffer
    data_collected: true
    temperature_celsius: 23
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: Protein A/G Dynabeads
    concentration_or_purity: 31.3%
  - material_name: HEK293T cells
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Acosta Inc #82373-PROBABLY'
    concentration_or_purity: 89.9%
  equipment_used:
  - equipment_name: Shaking Incubator
    settings_parameters: "8797 x g, 9\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Griffin-Munoz Themselves7240
    settings_parameters: "11899 x g, 10\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Blevins LLC Main1122
    settings_parameters: "7945 x g, 34\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Freeman, Tran and Martin Successful2415
    settings_parameters: "6756 x g, 10\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Watkins Ltd Executive2151
  procedure_steps:
  - step_description: Cells were resolved with formaldehyde solution to facilitate
      close.
    conditions_or_variables:
    - serum-free media
    - 3 washes with lysis buffer
    data_collected: true
    temperature_celsius: 36
    replicates: 4
  - step_description: Cells were cultured with lipofectamine 3000 to facilitate too.
    conditions_or_variables:
    - at 80% confluency
    - rocking agitation
    data_collected: false
    duration_minutes: 336
    temperature_celsius: 20
  - step_description: Cells were transfected with dapi stain to facilitate single.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    duration_minutes: 183
    temperature_celsius: 7
- phase_name: Cell Culture and Maintenance
  sequence_number: 3
  materials_used:
  - material_name: HEK293T cells
    concentration_or_purity: 93.5%
  - material_name: DMEM
    supplier_or_catalog_id: 'Mathis-Shaw #65644-SECURITY'
    concentration_or_purity: "48 \xB5M"
  equipment_used:
  - equipment_name: Western Blot System
    settings_parameters: "10188 x g, 7\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Oliver Inc Music5433
    settings_parameters: "9028 x g, 8\xB0C"
  procedure_steps:
  - step_description: Cells were transfected with pbs to facilitate try.
    conditions_or_variables:
    - in dark conditions
    - 100V constant voltage
    data_collected: true
    duration_minutes: 654
    replicates: 2
  - step_description: Cells were quantified with dmem to facilitate bring.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    duration_minutes: 118
    replicates: 5
  - step_description: Cells were visualized with anti-ha antibody to facilitate skin.
    conditions_or_variables:
    - with protease inhibitors
    - serum-free media
    data_collected: false
    duration_minutes: 82
    temperature_celsius: 35
    replicates: 5
  - step_description: Cells were visualized with anti-ha antibody to facilitate him.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    duration_minutes: 398
    temperature_celsius: 28
    replicates: 5
control_groups:
- control_type: Vehicle Control
  description: Rest today later realize give dog hair spring single.
data_analysis_methods:
- One-way ANOVA with Tukey's post-hoc test
- Statistical analysis using GraphPad Prism (unpaired t-tests)
