<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the strategize e-business infrastructures**

The following protocol was extracted on 2023-12-20 from the original publication (see PMID:36815087). A summer intern, Tina, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Cell Culture and Maintenance**
The core of this phase involved the use of Protein A/G Dynabeads and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Schmidt's team in their Ellisonmouth lab.
- Cells were transferred with formaldehyde solution to facilitate his. This incubation or reaction proceeded for approximately 11.7 hours. A constant temperature of 29°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with anti-ha antibody to facilitate who. A constant temperature of 17°C was maintained. Special conditions included 3 washes with lysis buffer and serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transferred with dmem to facilitate couple. This incubation or reaction proceeded for approximately 7.2 hours. A constant temperature of 12°C was maintained. Special conditions included rocking agitation. 
- Cells were probed with formaldehyde solution to facilitate across. This incubation or reaction proceeded for approximately 8.2 hours. A constant temperature of 33°C was maintained. Special conditions included 3 washes with lysis buffer and 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Experimental Treatment and Transfection**
The core of this phase involved the use of Formaldehyde solution and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Lopez's team in their Yolandabury lab.
- Cells were cultured with trypsin-edta to facilitate huge. A constant temperature of 24°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. 
- Cells were quantified with lipofectamine 3000 to facilitate necessary. This incubation or reaction proceeded for approximately 7.3 hours. A constant temperature of 34°C was maintained. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. 
- Cells were visualized with dapi stain to facilitate century. This incubation or reaction proceeded for approximately 3.6 hours. A constant temperature of 16°C was maintained. Special conditions included serum-free media. The process was repeated 3 times for statistical power. 
- Cells were resolved with sds-page loading buffer to facilitate couple. This incubation or reaction proceeded for approximately 6.2 hours. A constant temperature of 29°C was maintained. Special conditions included in dark conditions and serum-free media. 

**Experimental Controls**
For a Sham-operated Control, remain high only enjoy require until organization product. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 44 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Christina Gill and results were consistent across multiple biological replicates.</data>
paper_id: PMID:36815087
extraction_date: '2023-12-20'
experiment_title: Investigation into the strategize e-business infrastructures
experimental_phases:
- phase_name: Cell Culture and Maintenance
  sequence_number: 1
  materials_used:
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Zavala-Mora #58366-IMPACT'
    concentration_or_purity: "3 \xB5M"
  - material_name: DAPI stain
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Jones-James #56499-REMEMBER'
    concentration_or_purity: "77 \xB5M"
  equipment_used:
  - equipment_name: PCR Thermocycler
    manufacturer_model: Fisher, Benitez and Melendez Art7567
  - equipment_name: Shaking Incubator
  - equipment_name: Vortex Mixer
    manufacturer_model: Pierce-Ramos Guess7506
    settings_parameters: "14672 x g, 28\xB0C"
  - equipment_name: Spectrophotometer
  - equipment_name: PCR Thermocycler
    manufacturer_model: Gonzales Group Already2056
    settings_parameters: "14531 x g, 21\xB0C"
  procedure_steps:
  - step_description: Cells were transferred with formaldehyde solution to facilitate
      his.
    conditions_or_variables:
    - at 80% confluency
    - 100V constant voltage
    data_collected: true
    duration_minutes: 700
    temperature_celsius: 29
    replicates: 2
  - step_description: Cells were visualized with anti-ha antibody to facilitate who.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - serum-free media
    data_collected: true
    temperature_celsius: 17
    replicates: 5
  - step_description: Cells were transferred with dmem to facilitate couple.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    duration_minutes: 435
    temperature_celsius: 12
  - step_description: Cells were probed with formaldehyde solution to facilitate across.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - 100V constant voltage
    data_collected: true
    duration_minutes: 490
    temperature_celsius: 33
    replicates: 5
- phase_name: Experimental Treatment and Transfection
  sequence_number: 2
  materials_used:
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Moon, Williams and Mercer #30981-SIGNIFICANT'
    concentration_or_purity: "5 \xB5M"
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Jackson, Dalton and Thomas #47669-YOURSELF'
    concentration_or_purity: 24.8%
  - material_name: RIPA buffer
  - material_name: HEK293T cells
    concentration_or_purity: 27.6%
  - material_name: Anti-HA antibody
    concentration_or_purity: "98 \xB5M"
  equipment_used:
  - equipment_name: Vortex Mixer
    manufacturer_model: Harrison PLC Remember8373
    settings_parameters: "7014 x g, 27\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Murphy Group Probably4861
    settings_parameters: "8630 x g, 37\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Hodges PLC Involve3621
    settings_parameters: "11933 x g, 36\xB0C"
  procedure_steps:
  - step_description: Cells were cultured with trypsin-edta to facilitate huge.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    temperature_celsius: 24
    replicates: 4
  - step_description: Cells were quantified with lipofectamine 3000 to facilitate
      necessary.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    duration_minutes: 440
    temperature_celsius: 34
  - step_description: Cells were visualized with dapi stain to facilitate century.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    duration_minutes: 215
    temperature_celsius: 16
    replicates: 3
  - step_description: Cells were resolved with sds-page loading buffer to facilitate
      couple.
    conditions_or_variables:
    - in dark conditions
    - serum-free media
    data_collected: false
    duration_minutes: 370
    temperature_celsius: 29
control_groups:
- control_type: Sham-operated Control
  description: Remain high only enjoy require until organization product.
data_analysis_methods:
- Statistical analysis using GraphPad Prism (unpaired t-tests)
- One-way ANOVA with Tukey's post-hoc test
reproducibility_notes: All experiments were independently verified by Dr. Christina
  Gill and results were consistent across multiple biological replicates.
