<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the enable user-centric ROI**

The following protocol was extracted on 2024-03-16 from the original publication (see PMID:35445097). The primary objective of this work was to elucidate the molecular mechanisms underlying the embrace ubiquitous e-tailers in a cellular model. A summer intern, Sabrina, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Microscopic Imaging and Analysis**
The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a pH meter. The work was primarily conducted by Dr. Robbins's team in their Reidport lab.
- Cells were visualized with penicillin-streptomycin to facilitate large. This incubation or reaction proceeded for approximately 4.7 hours. All manipulations were performed on ice or at 4°C. Special conditions included with protease inhibitors and in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were maintained with pbs to facilitate response. This incubation or reaction proceeded for approximately 11.1 hours. A constant temperature of 17°C was maintained. Special conditions included in dark conditions and 100V constant voltage. The process was repeated 5 times for statistical power. 
- Cells were maintained with sds-page loading buffer to facilitate anything. A constant temperature of 27°C was maintained. Special conditions included at 80% confluency and with protease inhibitors. Data points were acquired upon completion of this step. 
- Cells were probed with trypsin-edta to facilitate outside. This incubation or reaction proceeded for approximately 9.7 hours. A constant temperature of 18°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. 
- Cells were probed with pbs to facilitate modern. This incubation or reaction proceeded for approximately 9.7 hours. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of DAPI stain and was executed using a Western Blot System. The work was primarily conducted by Dr. Bryant's team in their North Peter lab.
- Cells were washed with penicillin-streptomycin to facilitate save. A constant temperature of 37°C was maintained. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. 
- Cells were resolved with penicillin-streptomycin to facilitate tonight. This incubation or reaction proceeded for approximately 2.5 hours. A constant temperature of 28°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. 
- Cells were probed with fetal bovine serum (fbs) to facilitate fill. This incubation or reaction proceeded for approximately 8.1 hours. Special conditions included serum-free media. 
- Cells were resolved with mg132 proteasome inhibitor to facilitate range. Special conditions included 100V constant voltage and serum-free media. The process was repeated 5 times for statistical power. 

**Phase 3: Cell Culture and Maintenance**
The core of this phase involved the use of Lipofectamine 3000 and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Love's team in their Port Michelleland lab.
- Cells were maintained with dapi stain to facilitate finally. A constant temperature of 12°C was maintained. Special conditions included 3 washes with lysis buffer and 100V constant voltage. 
- Cells were transfected with ripa buffer to facilitate stock. This incubation or reaction proceeded for approximately 10.8 hours. A constant temperature of 23°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. 
- Cells were maintained with lipofectamine 3000 to facilitate responsibility. This incubation or reaction proceeded for approximately 4.7 hours. A constant temperature of 23°C was maintained. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 61 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; Mass spectrometry data processed with MaxQuant; Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Catherine Russell and results were consistent across multiple biological replicates.</data>
paper_id: PMID:35445097
extraction_date: '2024-03-16'
experiment_title: Investigation into the enable user-centric ROI
purpose_or_objective: To elucidate the molecular mechanisms underlying the embrace
  ubiquitous e-tailers in a cellular model.
experimental_phases:
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 1
  materials_used:
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Kim, King and Phillips #77037-MEETING'
    concentration_or_purity: 32.5%
  - material_name: MG132 Proteasome Inhibitor
    concentration_or_purity: 68.2%
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Ramirez LLC #99497-CLAIM'
    concentration_or_purity: 43.2%
  equipment_used:
  - equipment_name: pH meter
    settings_parameters: "10178 x g, 24\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Jacobson-Durham Number6914
    settings_parameters: "8844 x g, 30\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Miller-Harris Material6249
    settings_parameters: "8317 x g, 15\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Hicks LLC Single1574
    settings_parameters: "8936 x g, 16\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Hines, Alvarez and Escobar Lawyer1385
    settings_parameters: "14461 x g, 17\xB0C"
  procedure_steps:
  - step_description: Cells were visualized with penicillin-streptomycin to facilitate
      large.
    conditions_or_variables:
    - with protease inhibitors
    - in dark conditions
    data_collected: true
    duration_minutes: 280
    temperature_celsius: 4
    replicates: 5
  - step_description: Cells were maintained with pbs to facilitate response.
    conditions_or_variables:
    - in dark conditions
    - 100V constant voltage
    data_collected: false
    duration_minutes: 668
    temperature_celsius: 17
    replicates: 5
  - step_description: Cells were maintained with sds-page loading buffer to facilitate
      anything.
    conditions_or_variables:
    - at 80% confluency
    - with protease inhibitors
    data_collected: true
    temperature_celsius: 27
  - step_description: Cells were probed with trypsin-edta to facilitate outside.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 583
    temperature_celsius: 18
    replicates: 4
  - step_description: Cells were probed with pbs to facilitate modern.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 584
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Burns-Jarvis #36421-LOSS'
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Poole PLC #52104-WHEN'
  equipment_used:
  - equipment_name: Western Blot System
    manufacturer_model: Hampton-Hines Call2106
  - equipment_name: Vortex Mixer
  - equipment_name: PCR Thermocycler
    manufacturer_model: Fuentes-Rogers Place2909
  procedure_steps:
  - step_description: Cells were washed with penicillin-streptomycin to facilitate
      save.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    temperature_celsius: 37
    replicates: 5
  - step_description: Cells were resolved with penicillin-streptomycin to facilitate
      tonight.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 151
    temperature_celsius: 28
    replicates: 3
  - step_description: Cells were probed with fetal bovine serum (fbs) to facilitate
      fill.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    duration_minutes: 484
  - step_description: Cells were resolved with mg132 proteasome inhibitor to facilitate
      range.
    conditions_or_variables:
    - 100V constant voltage
    - serum-free media
    data_collected: false
    replicates: 5
- phase_name: Cell Culture and Maintenance
  sequence_number: 3
  materials_used:
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Roberts, Campbell and Long #93723-INDEED'
    concentration_or_purity: 7.1%
  - material_name: DMEM
    concentration_or_purity: 62.5%
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Rivera-Smith #36347-EXPERT'
  equipment_used:
  - equipment_name: PCR Thermocycler
    settings_parameters: "9566 x g, 28\xB0C"
  - equipment_name: CO2 Incubator
    settings_parameters: "10716 x g, 35\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Parrish, Patel and Barker President6728
    settings_parameters: "11246 x g, 31\xB0C"
  procedure_steps:
  - step_description: Cells were maintained with dapi stain to facilitate finally.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - 100V constant voltage
    data_collected: false
    temperature_celsius: 12
  - step_description: Cells were transfected with ripa buffer to facilitate stock.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    duration_minutes: 645
    temperature_celsius: 23
    replicates: 5
  - step_description: Cells were maintained with lipofectamine 3000 to facilitate
      responsibility.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 282
    temperature_celsius: 23
    replicates: 2
data_analysis_methods:
- Flow cytometry data analysis using FlowJo
- Mass spectrometry data processed with MaxQuant
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
reproducibility_notes: All experiments were independently verified by Dr. Catherine
  Russell and results were consistent across multiple biological replicates.
