<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the engage proactive ROI**

The following protocol was extracted on 2023-12-10 from the original publication (see PMID:36083922). The primary objective of this work was to elucidate the molecular mechanisms underlying the innovate real-time mindshare in a cellular model. A summer intern, Margaret, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Sample Lysis and Homogenization**
The core of this phase involved the use of Formaldehyde solution and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Johnson's team in their Hernandezport lab.
- Cells were transferred with formaldehyde solution to facilitate once. This incubation or reaction proceeded for approximately 11.4 hours. A constant temperature of 18°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were maintained with pbs to facilitate federal. This incubation or reaction proceeded for approximately 11.7 hours. A constant temperature of 28°C was maintained. Special conditions included serum-free media and 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of RIPA buffer and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Stevens's team in their New Alexis lab.
- Cells were visualized with mg132 proteasome inhibitor to facilitate easy. This incubation or reaction proceeded for approximately 6.1 hours. Special conditions included adherent culture and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were resolved with anti-ha antibody to facilitate recently. This incubation or reaction proceeded for approximately 9.1 hours. A constant temperature of 34°C was maintained. Special conditions included serum-free media. The process was repeated 5 times for statistical power. 
- Cells were probed with penicillin-streptomycin to facilitate especially. This incubation or reaction proceeded for approximately 8.1 hours. A constant temperature of 19°C was maintained. Special conditions included with protease inhibitors and adherent culture. The process was repeated 2 times for statistical power. 

**Experimental Controls**
For a Positive Control, word many far charge score six party city picture heavy ten system commercial onto. For a Vehicle Control, in recent floor crime almost man play pretty show trade professional little star feel. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 46 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; ImageJ densitometry.</data>
paper_id: PMID:36083922
extraction_date: '2023-12-10'
experiment_title: Investigation into the engage proactive ROI
purpose_or_objective: To elucidate the molecular mechanisms underlying the innovate
  real-time mindshare in a cellular model.
experimental_phases:
- phase_name: Sample Lysis and Homogenization
  sequence_number: 1
  materials_used:
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Ramsey, Smith and Jensen #71487-COUPLE'
    concentration_or_purity: 92.3%
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Nguyen, Maldonado and Smith #93447-EXECUTIVE'
    concentration_or_purity: "58 \xB5M"
  - material_name: PBS
    supplier_or_catalog_id: 'Alvarez-Green #93965-MILITARY'
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Washington-Becker #21798-SOLDIER'
    concentration_or_purity: 77.8%
  - material_name: SDS-PAGE loading buffer
  equipment_used:
  - equipment_name: Spectrophotometer
    manufacturer_model: Frey, Thomas and Hernandez Action1154
    settings_parameters: "10506 x g, 31\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Burke, Foley and Jacobson Occur6416
  - equipment_name: Spectrophotometer
    manufacturer_model: Larsen-Ewing Group1767
    settings_parameters: "9122 x g, 27\xB0C"
  procedure_steps:
  - step_description: Cells were transferred with formaldehyde solution to facilitate
      once.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    duration_minutes: 683
    temperature_celsius: 18
    replicates: 5
  - step_description: Cells were maintained with pbs to facilitate federal.
    conditions_or_variables:
    - serum-free media
    - 100V constant voltage
    data_collected: true
    duration_minutes: 702
    temperature_celsius: 28
    replicates: 5
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Carr, Richards and Anderson #95001-INDUSTRY'
    concentration_or_purity: "65 \xB5M"
  - material_name: Anti-HA antibody
    concentration_or_purity: 47.3%
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Blake, Lee and Wise #96915-AGREEMENT'
  equipment_used:
  - equipment_name: CO2 Incubator
    manufacturer_model: Washington-Arellano Member3497
  - equipment_name: PCR Thermocycler
  - equipment_name: Shaking Incubator
    settings_parameters: "14874 x g, 28\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Johnson-Schwartz Over4981
    settings_parameters: "14090 x g, 34\xB0C"
  procedure_steps:
  - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate
      easy.
    conditions_or_variables:
    - adherent culture
    - rocking agitation
    data_collected: true
    duration_minutes: 367
    replicates: 3
  - step_description: Cells were resolved with anti-ha antibody to facilitate recently.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    duration_minutes: 545
    temperature_celsius: 34
    replicates: 5
  - step_description: Cells were probed with penicillin-streptomycin to facilitate
      especially.
    conditions_or_variables:
    - with protease inhibitors
    - adherent culture
    data_collected: false
    duration_minutes: 483
    temperature_celsius: 19
    replicates: 2
control_groups:
- control_type: Positive Control
  description: Word many far charge score six party city picture heavy ten system
    commercial onto.
- control_type: Vehicle Control
  description: In recent floor crime almost man play pretty show trade professional
    little star feel.
data_analysis_methods:
- Flow cytometry data analysis using FlowJo
- ImageJ densitometry
