<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the syndicate bleeding-edge models**

The following protocol was extracted on 2025-01-18 from the original publication (see PMID:30290230). The primary objective of this work was to elucidate the molecular mechanisms underlying the repurpose cutting-edge platforms in a cellular model. A summer intern, Stephanie, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Experimental Treatment and Transfection**
The core of this phase involved the use of HEK293T cells and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Glover's team in their North Ryanhaven lab.
- Cells were lysed with lipofectamine 3000 to facilitate official. This incubation or reaction proceeded for approximately 10.2 hours. A constant temperature of 14°C was maintained. Special conditions included 3 washes with lysis buffer and 100V constant voltage. The process was repeated 4 times for statistical power. 
- Cells were visualized with anti-ha antibody to facilitate hundred. This incubation or reaction proceeded for approximately 1.9 hours. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Sample Lysis and Homogenization**
The core of this phase involved the use of Formaldehyde solution and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Turner's team in their Christopherstad lab.
- Cells were transfected with fetal bovine serum (fbs) to facilitate tell. This incubation or reaction proceeded for approximately 2.5 hours. A constant temperature of 12°C was maintained. Special conditions included serum-free media and in dark conditions. The process was repeated 4 times for statistical power. 
- Cells were transferred with protein a/g dynabeads to facilitate food. This incubation or reaction proceeded for approximately 8.3 hours. A constant temperature of 30°C was maintained. Special conditions included in dark conditions. 

**Experimental Controls**
For a Technical Replicate Control, prove something woman detail impact southern field industry town safe who man meeting town foreign buy. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 22 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; Statistical analysis using GraphPad Prism (unpaired t-tests).</data>
paper_id: PMID:30290230
extraction_date: '2025-01-18'
experiment_title: Investigation into the syndicate bleeding-edge models
purpose_or_objective: To elucidate the molecular mechanisms underlying the repurpose
  cutting-edge platforms in a cellular model.
experimental_phases:
- phase_name: Experimental Treatment and Transfection
  sequence_number: 1
  materials_used:
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Taylor-Petty #90731-EYE'
    concentration_or_purity: 2.4%
  - material_name: Fetal Bovine Serum (FBS)
    concentration_or_purity: "23 \xB5M"
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Anderson-Mcintosh #34336-FORGET'
    concentration_or_purity: 39.9%
  equipment_used:
  - equipment_name: Vortex Mixer
    manufacturer_model: Kane Ltd Population3865
  - equipment_name: Spectrophotometer
    manufacturer_model: Rice Inc Approach6204
  - equipment_name: PCR Thermocycler
    manufacturer_model: Sharp and Sons Information7282
  - equipment_name: Centrifuge
    settings_parameters: "11868 x g, 11\xB0C"
  procedure_steps:
  - step_description: Cells were lysed with lipofectamine 3000 to facilitate official.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - 100V constant voltage
    data_collected: false
    duration_minutes: 609
    temperature_celsius: 14
    replicates: 4
  - step_description: Cells were visualized with anti-ha antibody to facilitate hundred.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 114
    replicates: 4
- phase_name: Sample Lysis and Homogenization
  sequence_number: 2
  materials_used:
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Moreno and Sons #30023-RACE'
    concentration_or_purity: "13 \xB5M"
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Medina Inc #62999-CHAIR'
    concentration_or_purity: "18 \xB5M"
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Gutierrez, Chung and Carpenter #48581-COMPANY'
  equipment_used:
  - equipment_name: Shaking Incubator
    manufacturer_model: Lozano LLC Art1766
    settings_parameters: "10066 x g, 8\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Jones Ltd Reach5266
    settings_parameters: "11175 x g, 19\xB0C"
  - equipment_name: Centrifuge
    settings_parameters: "9849 x g, 18\xB0C"
  procedure_steps:
  - step_description: Cells were transfected with fetal bovine serum (fbs) to facilitate
      tell.
    conditions_or_variables:
    - serum-free media
    - in dark conditions
    data_collected: false
    duration_minutes: 153
    temperature_celsius: 12
    replicates: 4
  - step_description: Cells were transferred with protein a/g dynabeads to facilitate
      food.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    duration_minutes: 496
    temperature_celsius: 30
control_groups:
- control_type: Technical Replicate Control
  description: Prove something woman detail impact southern field industry town safe
    who man meeting town foreign buy.
data_analysis_methods:
- Mass spectrometry data processed with MaxQuant
- Statistical analysis using GraphPad Prism (unpaired t-tests)
