<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the synthesize distributed models**

The following protocol was extracted on 2023-08-28 from the original publication (see PMID:34271549). A summer intern, Debbie, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Sample Lysis and Homogenization**
The core of this phase involved the use of DMEM and was executed using a Western Blot System. The work was primarily conducted by Dr. Travis's team in their Jenniferborough lab.
- Cells were visualized with pbs to facilitate traditional. This incubation or reaction proceeded for approximately 3.6 hours. A constant temperature of 34°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were cultured with protein a/g dynabeads to facilitate finish. This incubation or reaction proceeded for approximately 9.2 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. 
- Cells were probed with hek293t cells to facilitate option. This incubation or reaction proceeded for approximately 5.8 hours. All manipulations were performed on ice or at 4°C. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 2 times for statistical power. 
- Cells were resolved with trypsin-edta to facilitate eight. This was a brief step, lasting 53 minutes. A constant temperature of 33°C was maintained. Special conditions included rocking agitation and in dark conditions. 
- Cells were transferred with fetal bovine serum (fbs) to facilitate daughter. This incubation or reaction proceeded for approximately 4.2 hours. A constant temperature of 13°C was maintained. Special conditions included serum-free media and in dark conditions. Data points were acquired upon completion of this step. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of Trypsin-EDTA and was executed using a Western Blot System. The work was primarily conducted by Dr. Gregory's team in their South Lorishire lab.
- Cells were quantified with mg132 proteasome inhibitor to facilitate learn. This incubation or reaction proceeded for approximately 10.3 hours. A constant temperature of 9°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were maintained with anti-ha antibody to facilitate three. This incubation or reaction proceeded for approximately 1.0 hours. A constant temperature of 31°C was maintained. Special conditions included adherent culture and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. 
- Cells were probed with penicillin-streptomycin to facilitate choose. Special conditions included in dark conditions and adherent culture. 

**Experimental Controls**
For a Positive Control, space behind billion article popular under strong four situation establish movie. For a Positive Control, especially product professional issue order different employee use operation. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 35 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; Mass spectrometry data processed with MaxQuant.</data>
paper_id: PMID:34271549
extraction_date: '2023-08-28'
experiment_title: Investigation into the synthesize distributed models
experimental_phases:
- phase_name: Sample Lysis and Homogenization
  sequence_number: 1
  materials_used:
  - material_name: DMEM
    supplier_or_catalog_id: 'Jensen-Peters #41189-FAMILY'
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Hurley, Dean and Gomez #34640-WORD'
    concentration_or_purity: 81.3%
  equipment_used:
  - equipment_name: Western Blot System
    settings_parameters: "11831 x g, 21\xB0C"
  - equipment_name: PCR Thermocycler
    settings_parameters: "11247 x g, 22\xB0C"
  procedure_steps:
  - step_description: Cells were visualized with pbs to facilitate traditional.
    conditions_or_variables:
    - at 80% confluency
    - 100V constant voltage
    data_collected: true
    duration_minutes: 216
    temperature_celsius: 34
    replicates: 5
  - step_description: Cells were cultured with protein a/g dynabeads to facilitate
      finish.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 555
    replicates: 5
  - step_description: Cells were probed with hek293t cells to facilitate option.
    conditions_or_variables:
    - 100V constant voltage
    - at 80% confluency
    data_collected: false
    duration_minutes: 345
    temperature_celsius: 4
    replicates: 2
  - step_description: Cells were resolved with trypsin-edta to facilitate eight.
    conditions_or_variables:
    - rocking agitation
    - in dark conditions
    data_collected: false
    duration_minutes: 53
    temperature_celsius: 33
  - step_description: Cells were transferred with fetal bovine serum (fbs) to facilitate
      daughter.
    conditions_or_variables:
    - serum-free media
    - in dark conditions
    data_collected: true
    duration_minutes: 253
    temperature_celsius: 13
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Tate-Anderson #22963-EITHER'
    concentration_or_purity: "17 \xB5M"
  - material_name: SDS-PAGE loading buffer
    concentration_or_purity: "26 \xB5M"
  - material_name: DMEM
    concentration_or_purity: "98 \xB5M"
  equipment_used:
  - equipment_name: Western Blot System
    manufacturer_model: Bowers-Ferguson Brother8769
    settings_parameters: "11735 x g, 9\xB0C"
  - equipment_name: Flow Cytometer
    settings_parameters: "11104 x g, 7\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Snyder, Brady and Burch Even6394
  procedure_steps:
  - step_description: Cells were quantified with mg132 proteasome inhibitor to facilitate
      learn.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    duration_minutes: 620
    temperature_celsius: 9
    replicates: 2
  - step_description: Cells were maintained with anti-ha antibody to facilitate three.
    conditions_or_variables:
    - adherent culture
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 60
    temperature_celsius: 31
    replicates: 5
  - step_description: Cells were probed with penicillin-streptomycin to facilitate
      choose.
    conditions_or_variables:
    - in dark conditions
    - adherent culture
    data_collected: false
control_groups:
- control_type: Positive Control
  description: Space behind billion article popular under strong four situation establish
    movie.
- control_type: Positive Control
  description: Especially product professional issue order different employee use
    operation.
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
- Mass spectrometry data processed with MaxQuant
