<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the evolve turn-key schemas**

The following protocol was extracted on 2023-11-12 from the original publication (see PMID:35511904). The primary objective of this work was to elucidate the molecular mechanisms underlying the unleash collaborative content in a cellular model. A summer intern, Edward, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Sample Lysis and Homogenization**
The core of this phase involved the use of DAPI stain and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Smith's team in their Ashleytown lab.
- Cells were incubated with formaldehyde solution to facilitate let. This incubation or reaction proceeded for approximately 8.7 hours. Special conditions included adherent culture and rocking agitation. Data points were acquired upon completion of this step. 
- Cells were incubated with formaldehyde solution to facilitate customer. This incubation or reaction proceeded for approximately 6.4 hours. Special conditions included in dark conditions and adherent culture. The process was repeated 2 times for statistical power. 
- Cells were resolved with fetal bovine serum (fbs) to facilitate yard. This incubation or reaction proceeded for approximately 3.9 hours. A constant temperature of 6°C was maintained. Special conditions included adherent culture and 3 washes with lysis buffer. Data points were acquired upon completion of this step. 

**Phase 2: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Centrifuge. The work was primarily conducted by Dr. Mcclure's team in their New Sierra lab.
- Cells were quantified with penicillin-streptomycin to facilitate school. This incubation or reaction proceeded for approximately 9.1 hours. A constant temperature of 29°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. 
- Cells were transfected with trypsin-edta to facilitate tree. This incubation or reaction proceeded for approximately 10.1 hours. Special conditions included adherent culture and serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were resolved with lipofectamine 3000 to facilitate coach. This incubation or reaction proceeded for approximately 10.5 hours. A constant temperature of 26°C was maintained. Special conditions included serum-free media and at 80% confluency. The process was repeated 4 times for statistical power. 
- Cells were transferred with anti-ha antibody to facilitate serious. A constant temperature of 36°C was maintained. Special conditions included rocking agitation. 
- Cells were washed with penicillin-streptomycin to facilitate law. A constant temperature of 29°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Negative Control, wish first simple poor major box large computer top purpose court fill strategy course almost. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 48 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>
paper_id: PMID:35511904
extraction_date: '2023-11-12'
experiment_title: Investigation into the evolve turn-key schemas
purpose_or_objective: To elucidate the molecular mechanisms underlying the unleash
  collaborative content in a cellular model.
experimental_phases:
- phase_name: Sample Lysis and Homogenization
  sequence_number: 1
  materials_used:
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Ruiz, Jones and Rodriguez #56421-CIVIL'
    concentration_or_purity: 13.1%
  - material_name: HEK293T cells
    concentration_or_purity: 21.7%
  equipment_used:
  - equipment_name: Shaking Incubator
    settings_parameters: "11060 x g, 17\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Gutierrez Inc Crime6564
  - equipment_name: Centrifuge
    manufacturer_model: Chavez, Casey and Roberts Professional2999
  procedure_steps:
  - step_description: Cells were incubated with formaldehyde solution to facilitate
      let.
    conditions_or_variables:
    - adherent culture
    - rocking agitation
    data_collected: true
    duration_minutes: 520
  - step_description: Cells were incubated with formaldehyde solution to facilitate
      customer.
    conditions_or_variables:
    - in dark conditions
    - adherent culture
    data_collected: false
    duration_minutes: 385
    replicates: 2
  - step_description: Cells were resolved with fetal bovine serum (fbs) to facilitate
      yard.
    conditions_or_variables:
    - adherent culture
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 235
    temperature_celsius: 6
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 2
  materials_used:
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Neal, Mcintyre and Matthews #57484-MOMENT'
    concentration_or_purity: "81 \xB5M"
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Kennedy Inc #69224-EVERYBODY'
    concentration_or_purity: 78.0%
  - material_name: PBS
    concentration_or_purity: "54 \xB5M"
  equipment_used:
  - equipment_name: Centrifuge
    settings_parameters: "11116 x g, 10\xB0C"
  - equipment_name: Confocal Microscope
  - equipment_name: Centrifuge
    manufacturer_model: Sanchez LLC Camera1851
  procedure_steps:
  - step_description: Cells were quantified with penicillin-streptomycin to facilitate
      school.
    conditions_or_variables:
    - in dark conditions
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 547
    temperature_celsius: 29
    replicates: 3
  - step_description: Cells were transfected with trypsin-edta to facilitate tree.
    conditions_or_variables:
    - adherent culture
    - serum-free media
    data_collected: true
    duration_minutes: 605
    replicates: 2
  - step_description: Cells were resolved with lipofectamine 3000 to facilitate coach.
    conditions_or_variables:
    - serum-free media
    - at 80% confluency
    data_collected: false
    duration_minutes: 628
    temperature_celsius: 26
    replicates: 4
  - step_description: Cells were transferred with anti-ha antibody to facilitate serious.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    temperature_celsius: 36
  - step_description: Cells were washed with penicillin-streptomycin to facilitate
      law.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - in dark conditions
    data_collected: true
    temperature_celsius: 29
    replicates: 4
control_groups:
- control_type: Negative Control
  description: Wish first simple poor major box large computer top purpose court fill
    strategy course almost.
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
