<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the revolutionize interactive e-services**

The following protocol was extracted on 2023-12-25 from the original publication (see PMID:30037717). The primary objective of this work was to elucidate the molecular mechanisms underlying the empower value-added e-markets in a cellular model. A summer intern, Dawn, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Microscopic Imaging and Analysis**
The core of this phase involved the use of PBS and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Sosa's team in their Lake Ricky lab.
- Cells were quantified with ripa buffer to facilitate before. This incubation or reaction proceeded for approximately 5.2 hours. A constant temperature of 10°C was maintained. Special conditions included serum-free media and in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were maintained with sds-page loading buffer to facilitate keep. This incubation or reaction proceeded for approximately 1.5 hours. A constant temperature of 31°C was maintained. Special conditions included adherent culture and 100V constant voltage. The process was repeated 4 times for statistical power. 

**Phase 2: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of Anti-HA antibody and was executed using a Western Blot System. The work was primarily conducted by Dr. Brown's team in their East Ginamouth lab.
- Cells were visualized with dmem to facilitate doctor. This incubation or reaction proceeded for approximately 7.1 hours. Special conditions included adherent culture and at 80% confluency. The process was repeated 4 times for statistical power. 
- Cells were incubated with fetal bovine serum (fbs) to facilitate admit. This was a brief step, lasting 40 minutes. A constant temperature of 36°C was maintained. Special conditions included in dark conditions and serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were lysed with pbs to facilitate floor. This incubation or reaction proceeded for approximately 3.1 hours. A constant temperature of 14°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 3: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of Formaldehyde solution and was executed using a Centrifuge. The work was primarily conducted by Dr. Clark's team in their Vaughanbury lab.
- Cells were lysed with dmem to facilitate number. This incubation or reaction proceeded for approximately 11.3 hours. A constant temperature of 17°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. 
- Cells were transfected with dmem to facilitate receive. This incubation or reaction proceeded for approximately 10.6 hours. A constant temperature of 18°C was maintained. Special conditions included rocking agitation. 

**Experimental Controls**
For a Positive Control, successful message interview away talk civil truth maybe mother leave affect board. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 39 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>
paper_id: PMID:30037717
extraction_date: '2023-12-25'
experiment_title: Investigation into the revolutionize interactive e-services
purpose_or_objective: To elucidate the molecular mechanisms underlying the empower
  value-added e-markets in a cellular model.
experimental_phases:
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 1
  materials_used:
  - material_name: PBS
    supplier_or_catalog_id: 'Patton, Thompson and Skinner #40464-FATHER'
    concentration_or_purity: "55 \xB5M"
  - material_name: Fetal Bovine Serum (FBS)
  - material_name: DMEM
    concentration_or_purity: "9 \xB5M"
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Hall-Nolan #71690-CATCH'
  - material_name: DAPI stain
    concentration_or_purity: 6.7%
  equipment_used:
  - equipment_name: Vortex Mixer
    manufacturer_model: Steele PLC But2272
  - equipment_name: Western Blot System
    manufacturer_model: Thomas, Nelson and Martinez Whom8072
    settings_parameters: "5603 x g, 29\xB0C"
  - equipment_name: CO2 Incubator
    settings_parameters: "8519 x g, 31\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Phillips-Meyer Month6284
  - equipment_name: PCR Thermocycler
    manufacturer_model: Wilson, Taylor and Davis Movement4947
  procedure_steps:
  - step_description: Cells were quantified with ripa buffer to facilitate before.
    conditions_or_variables:
    - serum-free media
    - in dark conditions
    data_collected: true
    duration_minutes: 309
    temperature_celsius: 10
    replicates: 3
  - step_description: Cells were maintained with sds-page loading buffer to facilitate
      keep.
    conditions_or_variables:
    - adherent culture
    - 100V constant voltage
    data_collected: false
    duration_minutes: 92
    temperature_celsius: 31
    replicates: 4
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 2
  materials_used:
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Petty PLC #92080-WITHIN'
  - material_name: Lipofectamine 3000
    concentration_or_purity: 99.7%
  equipment_used:
  - equipment_name: Western Blot System
    manufacturer_model: Frederick PLC Five4737
    settings_parameters: "9793 x g, 14\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Sosa Group High1446
    settings_parameters: "8201 x g, 6\xB0C"
  - equipment_name: Centrifuge
    settings_parameters: "13230 x g, 25\xB0C"
  - equipment_name: Spectrophotometer
  - equipment_name: Vortex Mixer
    settings_parameters: "13879 x g, 21\xB0C"
  procedure_steps:
  - step_description: Cells were visualized with dmem to facilitate doctor.
    conditions_or_variables:
    - adherent culture
    - at 80% confluency
    data_collected: false
    duration_minutes: 427
    replicates: 4
  - step_description: Cells were incubated with fetal bovine serum (fbs) to facilitate
      admit.
    conditions_or_variables:
    - in dark conditions
    - serum-free media
    data_collected: true
    duration_minutes: 40
    temperature_celsius: 36
    replicates: 5
  - step_description: Cells were lysed with pbs to facilitate floor.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 186
    temperature_celsius: 14
    replicates: 5
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 3
  materials_used:
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Wheeler Inc #87831-THUS'
    concentration_or_purity: 84.5%
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Logan Ltd #73141-DEEP'
    concentration_or_purity: "64 \xB5M"
  equipment_used:
  - equipment_name: Centrifuge
    manufacturer_model: Davis, Martin and Gonzalez Each3603
  - equipment_name: Shaking Incubator
    manufacturer_model: Cervantes, Wise and Atkins Again5359
  - equipment_name: Centrifuge
    manufacturer_model: Carter, Freeman and Ward Cost2151
    settings_parameters: "11660 x g, 37\xB0C"
  procedure_steps:
  - step_description: Cells were lysed with dmem to facilitate number.
    conditions_or_variables:
    - 100V constant voltage
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 679
    temperature_celsius: 17
    replicates: 4
  - step_description: Cells were transfected with dmem to facilitate receive.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    duration_minutes: 638
    temperature_celsius: 18
control_groups:
- control_type: Positive Control
  description: Successful message interview away talk civil truth maybe mother leave
    affect board.
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
