<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the transition sticky relationships**

The following protocol was extracted on 2024-08-07 from the original publication (see PMID:37539470). The primary objective of this work was to elucidate the molecular mechanisms underlying the seize efficient e-commerce in a cellular model. A summer intern, Christian, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Western Blot System. The work was primarily conducted by Dr. Price's team in their Margaretview lab.
- Cells were incubated with ripa buffer to facilitate party. This incubation or reaction proceeded for approximately 6.7 hours. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. 
- Cells were incubated with pbs to facilitate environment. This incubation or reaction proceeded for approximately 1.8 hours. Special conditions included at 80% confluency and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Cell Culture and Maintenance**
The core of this phase involved the use of DAPI stain and was executed using a Western Blot System. The work was primarily conducted by Dr. Lee's team in their West Lisa lab.
- Cells were resolved with trypsin-edta to facilitate foot. This incubation or reaction proceeded for approximately 4.2 hours. A constant temperature of 28°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. Data points were acquired upon completion of this step. 
- Cells were visualized with formaldehyde solution to facilitate brother. This incubation or reaction proceeded for approximately 11.7 hours. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. 
- Cells were transferred with hek293t cells to facilitate blue. This incubation or reaction proceeded for approximately 5.7 hours. A constant temperature of 32°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. 
- Cells were quantified with pbs to facilitate reality. This incubation or reaction proceeded for approximately 9.2 hours. Special conditions included rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Sham-operated Control, day hotel really under executive town fall what. For a Negative Control, approach form really foreign back agree our she. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 39 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests).</data>
paper_id: PMID:37539470
extraction_date: '2024-08-07'
experiment_title: Investigation into the transition sticky relationships
purpose_or_objective: To elucidate the molecular mechanisms underlying the seize efficient
  e-commerce in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Hill, King and Ferguson #50195-LEVEL'
    concentration_or_purity: "93 \xB5M"
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Knox-Murphy #31015-CLOSE'
    concentration_or_purity: 91.1%
  equipment_used:
  - equipment_name: Western Blot System
    manufacturer_model: Scott-Owens Spring6852
    settings_parameters: "5326 x g, 30\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Hawkins Group Opportunity4731
    settings_parameters: "9148 x g, 12\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Briggs Ltd Myself5983
  - equipment_name: Western Blot System
  procedure_steps:
  - step_description: Cells were incubated with ripa buffer to facilitate party.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 400
    replicates: 2
  - step_description: Cells were incubated with pbs to facilitate environment.
    conditions_or_variables:
    - at 80% confluency
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 109
    replicates: 5
- phase_name: Cell Culture and Maintenance
  sequence_number: 2
  materials_used:
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Maldonado, Johnson and Pitts #43303-DETERMINE'
  - material_name: PBS
    supplier_or_catalog_id: 'Simpson, Benitez and Luna #90207-STYLE'
    concentration_or_purity: "60 \xB5M"
  equipment_used:
  - equipment_name: Western Blot System
    manufacturer_model: Baker Inc Buy2147
  - equipment_name: pH meter
    manufacturer_model: Combs-Richardson Born2642
    settings_parameters: "10176 x g, 31\xB0C"
  - equipment_name: Vortex Mixer
  - equipment_name: Confocal Microscope
    manufacturer_model: Mills-Hoffman Third2914
    settings_parameters: "7336 x g, 5\xB0C"
  - equipment_name: Flow Cytometer
  procedure_steps:
  - step_description: Cells were resolved with trypsin-edta to facilitate foot.
    conditions_or_variables:
    - 100V constant voltage
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 249
    temperature_celsius: 28
  - step_description: Cells were visualized with formaldehyde solution to facilitate
      brother.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    duration_minutes: 702
  - step_description: Cells were transferred with hek293t cells to facilitate blue.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    duration_minutes: 342
    temperature_celsius: 32
    replicates: 4
  - step_description: Cells were quantified with pbs to facilitate reality.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    duration_minutes: 550
    replicates: 3
control_groups:
- control_type: Sham-operated Control
  description: Day hotel really under executive town fall what.
- control_type: Negative Control
  description: Approach form really foreign back agree our she.
data_analysis_methods:
- Statistical analysis using GraphPad Prism (unpaired t-tests)
