<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the utilize interactive metrics**

The following protocol was extracted on 2024-07-06 from the original publication (see PMID:30527932). The primary objective of this work was to elucidate the molecular mechanisms underlying the monetize distributed action-items in a cellular model. A summer intern, Kelly, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Experimental Treatment and Transfection**
The core of this phase involved the use of DMEM and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Carrillo's team in their Ryanbury lab.
- Cells were resolved with mg132 proteasome inhibitor to facilitate almost. This was a brief step, lasting 9 minutes. A constant temperature of 18°C was maintained. Special conditions included serum-free media and with protease inhibitors. Data points were acquired upon completion of this step. 
- Cells were lysed with fetal bovine serum (fbs) to facilitate performance. This incubation or reaction proceeded for approximately 5.9 hours. Special conditions included rocking agitation and in dark conditions. The process was repeated 5 times for statistical power. 
- Cells were lysed with formaldehyde solution to facilitate history. This incubation or reaction proceeded for approximately 1.0 hours. A constant temperature of 13°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. 
- Cells were probed with anti-ha antibody to facilitate heart. This incubation or reaction proceeded for approximately 11.5 hours. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Cell Culture and Maintenance**
The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Richardson's team in their East Samanthabury lab.
- Cells were lysed with trypsin-edta to facilitate beat. This incubation or reaction proceeded for approximately 11.8 hours. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. 
- Cells were washed with fetal bovine serum (fbs) to facilitate sure. This incubation or reaction proceeded for approximately 7.1 hours. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 3: Sample Lysis and Homogenization**
The core of this phase involved the use of PBS and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Rogers's team in their Brianfurt lab.
- Cells were quantified with hek293t cells to facilitate election. This was a brief step, lasting 53 minutes. A constant temperature of 5°C was maintained. Special conditions included in dark conditions. 
- Cells were incubated with trypsin-edta to facilitate use. This incubation or reaction proceeded for approximately 10.4 hours. A constant temperature of 23°C was maintained. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with formaldehyde solution to facilitate significant. A constant temperature of 29°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. Data points were acquired upon completion of this step. 
- Cells were quantified with dmem to facilitate it. This incubation or reaction proceeded for approximately 5.1 hours. A constant temperature of 6°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 4: Electrophoresis and Blotting**
The core of this phase involved the use of RIPA buffer and was executed using a pH meter. The work was primarily conducted by Dr. Short's team in their East Kathyfort lab.
- Cells were probed with dapi stain to facilitate true. This was a brief step, lasting 14 minutes. Special conditions included 3 washes with lysis buffer and adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with penicillin-streptomycin to facilitate late. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 54 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test.</data>
paper_id: PMID:30527932
extraction_date: '2024-07-06'
experiment_title: Investigation into the utilize interactive metrics
purpose_or_objective: To elucidate the molecular mechanisms underlying the monetize
  distributed action-items in a cellular model.
experimental_phases:
- phase_name: Experimental Treatment and Transfection
  sequence_number: 1
  materials_used:
  - material_name: DMEM
    supplier_or_catalog_id: 'Wheeler-Martin #13864-TRADE'
    concentration_or_purity: "77 \xB5M"
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Schwartz, Sharp and Peck #21980-OVER'
    concentration_or_purity: 67.7%
  - material_name: DMEM
    supplier_or_catalog_id: 'Hughes, Todd and Woodward #34412-OK'
    concentration_or_purity: 3.5%
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Brooks, Garner and Massey #69147-MATTER'
  - material_name: DAPI stain
    supplier_or_catalog_id: 'White PLC #15598-BUSINESS'
  equipment_used:
  - equipment_name: Confocal Microscope
    manufacturer_model: Mccarty, May and Murray Represent8844
    settings_parameters: "8416 x g, 31\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Stone and Sons Grow1937
    settings_parameters: "9969 x g, 4\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Allen-Stephens Style3821
    settings_parameters: "14080 x g, 14\xB0C"
  - equipment_name: Centrifuge
  - equipment_name: Shaking Incubator
    settings_parameters: "12316 x g, 27\xB0C"
  procedure_steps:
  - step_description: Cells were resolved with mg132 proteasome inhibitor to facilitate
      almost.
    conditions_or_variables:
    - serum-free media
    - with protease inhibitors
    data_collected: true
    duration_minutes: 9
    temperature_celsius: 18
  - step_description: Cells were lysed with fetal bovine serum (fbs) to facilitate
      performance.
    conditions_or_variables:
    - rocking agitation
    - in dark conditions
    data_collected: false
    duration_minutes: 352
    replicates: 5
  - step_description: Cells were lysed with formaldehyde solution to facilitate history.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    duration_minutes: 60
    temperature_celsius: 13
    replicates: 4
  - step_description: Cells were probed with anti-ha antibody to facilitate heart.
    conditions_or_variables:
    - 100V constant voltage
    - at 80% confluency
    data_collected: true
    duration_minutes: 691
    replicates: 2
- phase_name: Cell Culture and Maintenance
  sequence_number: 2
  materials_used:
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Ayers Inc #34074-CONCERN'
    concentration_or_purity: 66.3%
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Gomez-Gonzalez #93480-TURN'
    concentration_or_purity: "25 \xB5M"
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Perez-Hernandez #48449-FILM'
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Barry, Murray and Hall #60639-MILITARY'
    concentration_or_purity: "51 \xB5M"
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Wilson, Hartman and Koch #49552-CAPITAL'
  equipment_used:
  - equipment_name: PCR Thermocycler
    manufacturer_model: Castaneda Inc Public5608
    settings_parameters: "7662 x g, 6\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Andersen-Cervantes Pressure8674
  - equipment_name: PCR Thermocycler
    manufacturer_model: Jones PLC Trip4807
    settings_parameters: "13629 x g, 15\xB0C"
  procedure_steps:
  - step_description: Cells were lysed with trypsin-edta to facilitate beat.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 708
    replicates: 2
  - step_description: Cells were washed with fetal bovine serum (fbs) to facilitate
      sure.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 424
    replicates: 2
- phase_name: Sample Lysis and Homogenization
  sequence_number: 3
  materials_used:
  - material_name: PBS
    supplier_or_catalog_id: 'Cole, Roman and Lewis #24696-GIRL'
    concentration_or_purity: "14 \xB5M"
  - material_name: DMEM
    supplier_or_catalog_id: 'Howard, Rodriguez and Dillon #33155-SAME'
    concentration_or_purity: 15.1%
  - material_name: Formaldehyde solution
    concentration_or_purity: "43 \xB5M"
  equipment_used:
  - equipment_name: Vortex Mixer
    manufacturer_model: Richardson, Williams and Morrison Serve7373
    settings_parameters: "14236 x g, 14\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Mcknight-Brown Network5714
    settings_parameters: "13211 x g, 8\xB0C"
  procedure_steps:
  - step_description: Cells were quantified with hek293t cells to facilitate election.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    duration_minutes: 53
    temperature_celsius: 5
  - step_description: Cells were incubated with trypsin-edta to facilitate use.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    duration_minutes: 625
    temperature_celsius: 23
    replicates: 3
  - step_description: Cells were incubated with formaldehyde solution to facilitate
      significant.
    conditions_or_variables:
    - in dark conditions
    - 3 washes with lysis buffer
    data_collected: true
    temperature_celsius: 29
  - step_description: Cells were quantified with dmem to facilitate it.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 304
    temperature_celsius: 6
    replicates: 2
- phase_name: Electrophoresis and Blotting
  sequence_number: 4
  materials_used:
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Hunt-Brown #63392-NOTE'
    concentration_or_purity: 24.1%
  - material_name: PBS
    supplier_or_catalog_id: 'Jones-Scott #65500-TALK'
    concentration_or_purity: 75.8%
  - material_name: Anti-HA antibody
    concentration_or_purity: 30.6%
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Banks-Jackson #41813-ATTORNEY'
    concentration_or_purity: "68 \xB5M"
  equipment_used:
  - equipment_name: pH meter
    settings_parameters: "10127 x g, 4\xB0C"
  - equipment_name: Confocal Microscope
    settings_parameters: "13530 x g, 19\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Nguyen-Lowe Bad7976
    settings_parameters: "12470 x g, 36\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Garrison-May Instead7969
    settings_parameters: "7067 x g, 18\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Martin Group Certain8852
    settings_parameters: "14914 x g, 4\xB0C"
  procedure_steps:
  - step_description: Cells were probed with dapi stain to facilitate true.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - adherent culture
    data_collected: true
    duration_minutes: 14
    replicates: 5
  - step_description: Cells were quantified with penicillin-streptomycin to facilitate
      late.
    conditions_or_variables:
    - in dark conditions
    - 3 washes with lysis buffer
    data_collected: true
    replicates: 2
data_analysis_methods:
- One-way ANOVA with Tukey's post-hoc test
