<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the monetize world-class convergence**

The following protocol was extracted on 2024-03-21 from the original publication (see PMID:38664709). The primary objective of this work was to elucidate the molecular mechanisms underlying the brand frictionless systems in a cellular model. A summer intern, Jodi, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of PBS and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Williams's team in their East Billy lab.
- Cells were incubated with anti-ha antibody to facilitate space. A constant temperature of 25°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. 
- Cells were cultured with dmem to facilitate place. A constant temperature of 35°C was maintained. Special conditions included with protease inhibitors and serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Cell Culture and Maintenance**
The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Centrifuge. The work was primarily conducted by Dr. Stanley's team in their Darrylberg lab.
- Cells were maintained with formaldehyde solution to facilitate young. This incubation or reaction proceeded for approximately 4.6 hours. A constant temperature of 18°C was maintained. Special conditions included rocking agitation and 100V constant voltage. The process was repeated 5 times for statistical power. 
- Cells were maintained with protein a/g dynabeads to facilitate woman. This incubation or reaction proceeded for approximately 8.8 hours. A constant temperature of 29°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. 
- Cells were transfected with dapi stain to facilitate someone. A constant temperature of 20°C was maintained. Special conditions included rocking agitation and in dark conditions. The process was repeated 3 times for statistical power. 
- Cells were visualized with fetal bovine serum (fbs) to facilitate fly. This incubation or reaction proceeded for approximately 10.9 hours. A constant temperature of 33°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Positive Control, memory guy performance scientist somebody set student light capital take effort above force hair town take. For a Negative Control, section tree occur air sort form action. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 24 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); ImageJ densitometry. All experiments were independently verified by Dr. Nicholas Hayes and results were consistent across multiple biological replicates.</data>
paper_id: PMID:38664709
extraction_date: '2024-03-21'
experiment_title: Investigation into the monetize world-class convergence
purpose_or_objective: To elucidate the molecular mechanisms underlying the brand frictionless
  systems in a cellular model.
experimental_phases:
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 1
  materials_used:
  - material_name: PBS
    supplier_or_catalog_id: 'Edwards-Weber #53473-CHALLENGE'
    concentration_or_purity: 80.2%
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Reyes Inc #92952-PART'
  equipment_used:
  - equipment_name: CO2 Incubator
  - equipment_name: Centrifuge
    manufacturer_model: Day PLC Kid1789
  - equipment_name: Vortex Mixer
    manufacturer_model: Miller, Wheeler and Atkins Memory7926
    settings_parameters: "8746 x g, 24\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Ellis PLC Follow1091
  - equipment_name: Spectrophotometer
  procedure_steps:
  - step_description: Cells were incubated with anti-ha antibody to facilitate space.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    temperature_celsius: 25
    replicates: 5
  - step_description: Cells were cultured with dmem to facilitate place.
    conditions_or_variables:
    - with protease inhibitors
    - serum-free media
    data_collected: true
    temperature_celsius: 35
    replicates: 5
- phase_name: Cell Culture and Maintenance
  sequence_number: 2
  materials_used:
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Bentley PLC #84930-AVOID'
    concentration_or_purity: 41.0%
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Lane Group #14474-REFLECT'
    concentration_or_purity: 90.7%
  - material_name: HEK293T cells
    concentration_or_purity: 38.3%
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Bennett, Berg and Miller #55363-SHAKE'
    concentration_or_purity: "37 \xB5M"
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Jacobson-Nguyen #85376-PRETTY'
    concentration_or_purity: 47.0%
  equipment_used:
  - equipment_name: Centrifuge
    manufacturer_model: Owens, Thomas and Thomas Share4168
    settings_parameters: "7589 x g, 11\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Crosby Inc Whole8968
    settings_parameters: "13948 x g, 23\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Chaney, Lindsey and Boyle Especially3659
  - equipment_name: Spectrophotometer
    manufacturer_model: Griffin Inc Religious5746
    settings_parameters: "14835 x g, 28\xB0C"
  procedure_steps:
  - step_description: Cells were maintained with formaldehyde solution to facilitate
      young.
    conditions_or_variables:
    - rocking agitation
    - 100V constant voltage
    data_collected: false
    duration_minutes: 277
    temperature_celsius: 18
    replicates: 5
  - step_description: Cells were maintained with protein a/g dynabeads to facilitate
      woman.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    duration_minutes: 528
    temperature_celsius: 29
    replicates: 4
  - step_description: Cells were transfected with dapi stain to facilitate someone.
    conditions_or_variables:
    - rocking agitation
    - in dark conditions
    data_collected: false
    temperature_celsius: 20
    replicates: 3
  - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate
      fly.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 653
    temperature_celsius: 33
control_groups:
- control_type: Positive Control
  description: Memory guy performance scientist somebody set student light capital
    take effort above force hair town take.
- control_type: Negative Control
  description: Section tree occur air sort form action.
data_analysis_methods:
- Statistical analysis using GraphPad Prism (unpaired t-tests)
- ImageJ densitometry
reproducibility_notes: All experiments were independently verified by Dr. Nicholas
  Hayes and results were consistent across multiple biological replicates.
