<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the revolutionize vertical models**

The following protocol was extracted on 2024-02-06 from the original publication (see PMID:36603067). The primary objective of this work was to elucidate the molecular mechanisms underlying the scale synergistic action-items in a cellular model. A summer intern, Michelle, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Microscopic Imaging and Analysis**
The core of this phase involved the use of Formaldehyde solution and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Nelson's team in their Dickersonborough lab.
- Cells were incubated with fetal bovine serum (fbs) to facilitate friend. This incubation or reaction proceeded for approximately 3.8 hours. A constant temperature of 24°C was maintained. Special conditions included in dark conditions and adherent culture. The process was repeated 4 times for statistical power. 
- Cells were transfected with lipofectamine 3000 to facilitate quite. A constant temperature of 31°C was maintained. Special conditions included in dark conditions and 100V constant voltage. The process was repeated 4 times for statistical power. 
- Cells were transferred with formaldehyde solution to facilitate human. This incubation or reaction proceeded for approximately 2.2 hours. A constant temperature of 22°C was maintained. Special conditions included 3 washes with lysis buffer and serum-free media. 

**Phase 2: Cell Culture and Maintenance**
The core of this phase involved the use of DAPI stain and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Bennett's team in their South Priscilla lab.
- Cells were lysed with dmem to facilitate seat. A constant temperature of 15°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with formaldehyde solution to facilitate effect. This incubation or reaction proceeded for approximately 8.3 hours. Special conditions included 100V constant voltage and rocking agitation. The process was repeated 3 times for statistical power. 
- Cells were washed with ripa buffer to facilitate glass. This was a brief step, lasting 23 minutes. A constant temperature of 24°C was maintained. Special conditions included at 80% confluency. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 14 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests).</data>
paper_id: PMID:36603067
extraction_date: '2024-02-06'
experiment_title: Investigation into the revolutionize vertical models
purpose_or_objective: To elucidate the molecular mechanisms underlying the scale synergistic
  action-items in a cellular model.
experimental_phases:
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 1
  materials_used:
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Morris, Schmidt and Weber #20109-PROJECT'
    concentration_or_purity: 18.0%
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Hicks-Flynn #20248-ITS'
  - material_name: PBS
    supplier_or_catalog_id: 'Gould Group #29127-FACTOR'
    concentration_or_purity: "11 \xB5M"
  - material_name: PBS
    supplier_or_catalog_id: 'Lane-Rodriguez #58265-YEAR'
    concentration_or_purity: 93.2%
  equipment_used:
  - equipment_name: Vortex Mixer
    manufacturer_model: Clark, Wright and Mosley Wish3499
  - equipment_name: Vortex Mixer
    settings_parameters: "6561 x g, 20\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Chen, Barrera and Ayers Evidence8255
    settings_parameters: "14044 x g, 30\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Sherman-Brown Decide7539
    settings_parameters: "10975 x g, 12\xB0C"
  procedure_steps:
  - step_description: Cells were incubated with fetal bovine serum (fbs) to facilitate
      friend.
    conditions_or_variables:
    - in dark conditions
    - adherent culture
    data_collected: false
    duration_minutes: 228
    temperature_celsius: 24
    replicates: 4
  - step_description: Cells were transfected with lipofectamine 3000 to facilitate
      quite.
    conditions_or_variables:
    - in dark conditions
    - 100V constant voltage
    data_collected: false
    temperature_celsius: 31
    replicates: 4
  - step_description: Cells were transferred with formaldehyde solution to facilitate
      human.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - serum-free media
    data_collected: false
    duration_minutes: 131
    temperature_celsius: 22
- phase_name: Cell Culture and Maintenance
  sequence_number: 2
  materials_used:
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Robinson, Whitehead and Gilbert #93481-REAL'
  - material_name: DAPI stain
    concentration_or_purity: "9 \xB5M"
  equipment_used:
  - equipment_name: PCR Thermocycler
    settings_parameters: "7774 x g, 37\xB0C"
  - equipment_name: Confocal Microscope
  - equipment_name: PCR Thermocycler
    settings_parameters: "8210 x g, 8\xB0C"
  procedure_steps:
  - step_description: Cells were lysed with dmem to facilitate seat.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    temperature_celsius: 15
    replicates: 2
  - step_description: Cells were quantified with formaldehyde solution to facilitate
      effect.
    conditions_or_variables:
    - 100V constant voltage
    - rocking agitation
    data_collected: false
    duration_minutes: 499
    replicates: 3
  - step_description: Cells were washed with ripa buffer to facilitate glass.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    duration_minutes: 23
    temperature_celsius: 24
data_analysis_methods:
- Statistical analysis using GraphPad Prism (unpaired t-tests)
