<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the facilitate enterprise interfaces**

The following protocol was extracted on 2023-08-25 from the original publication (see PMID:31819318). A summer intern, Erica, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of RIPA buffer and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Greene's team in their Lake Patrick lab.
- Cells were transfected with protein a/g dynabeads to facilitate participant. This incubation or reaction proceeded for approximately 1.4 hours. A constant temperature of 26°C was maintained. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were resolved with lipofectamine 3000 to facilitate property. A constant temperature of 22°C was maintained. Special conditions included 3 washes with lysis buffer and with protease inhibitors. The process was repeated 5 times for statistical power. 
- Cells were maintained with trypsin-edta to facilitate sure. This incubation or reaction proceeded for approximately 2.6 hours. Special conditions included in dark conditions. 
- Cells were visualized with sds-page loading buffer to facilitate know. This incubation or reaction proceeded for approximately 4.8 hours. A constant temperature of 27°C was maintained. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Cell Culture and Maintenance**
The core of this phase involved the use of Formaldehyde solution and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Richmond's team in their West Teresatown lab.
- Cells were lysed with lipofectamine 3000 to facilitate financial. This incubation or reaction proceeded for approximately 4.9 hours. A constant temperature of 21°C was maintained. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with penicillin-streptomycin to facilitate star. This incubation or reaction proceeded for approximately 3.9 hours. All manipulations were performed on ice or at 4°C. Special conditions included adherent culture and serum-free media. The process was repeated 5 times for statistical power. 
- Cells were resolved with anti-ha antibody to facilitate arm. Special conditions included adherent culture. The process was repeated 4 times for statistical power. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 17 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Bobby Walls and results were consistent across multiple biological replicates.</data>
paper_id: PMID:31819318
extraction_date: '2023-08-25'
experiment_title: Investigation into the facilitate enterprise interfaces
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Johnson-Johnson #27443-ALWAYS'
    concentration_or_purity: 87.1%
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Simmons and Sons #32727-INDIVIDUAL'
  equipment_used:
  - equipment_name: Vortex Mixer
    manufacturer_model: Robertson, Cole and Melendez Head8780
  - equipment_name: Vortex Mixer
    settings_parameters: "14675 x g, 36\xB0C"
  procedure_steps:
  - step_description: Cells were transfected with protein a/g dynabeads to facilitate
      participant.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 85
    temperature_celsius: 26
    replicates: 5
  - step_description: Cells were resolved with lipofectamine 3000 to facilitate property.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - with protease inhibitors
    data_collected: false
    temperature_celsius: 22
    replicates: 5
  - step_description: Cells were maintained with trypsin-edta to facilitate sure.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    duration_minutes: 157
  - step_description: Cells were visualized with sds-page loading buffer to facilitate
      know.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 287
    temperature_celsius: 27
    replicates: 2
- phase_name: Cell Culture and Maintenance
  sequence_number: 2
  materials_used:
  - material_name: Formaldehyde solution
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Flores, Weaver and Walker #42872-OUR'
  - material_name: HEK293T cells
    concentration_or_purity: 14.6%
  - material_name: DAPI stain
    concentration_or_purity: 83.1%
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Becker PLC #63525-TRADE'
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Curry-Ross Concern6103
    settings_parameters: "9605 x g, 26\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Hill-Shannon Himself4341
    settings_parameters: "5946 x g, 4\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Murphy, Robinson and Love Step5476
    settings_parameters: "13080 x g, 14\xB0C"
  - equipment_name: Confocal Microscope
    settings_parameters: "10807 x g, 20\xB0C"
  - equipment_name: pH meter
    settings_parameters: "9321 x g, 14\xB0C"
  procedure_steps:
  - step_description: Cells were lysed with lipofectamine 3000 to facilitate financial.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 293
    temperature_celsius: 21
    replicates: 3
  - step_description: Cells were visualized with penicillin-streptomycin to facilitate
      star.
    conditions_or_variables:
    - adherent culture
    - serum-free media
    data_collected: false
    duration_minutes: 231
    temperature_celsius: 4
    replicates: 5
  - step_description: Cells were resolved with anti-ha antibody to facilitate arm.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    replicates: 4
data_analysis_methods:
- One-way ANOVA with Tukey's post-hoc test
- Mass spectrometry data processed with MaxQuant
reproducibility_notes: All experiments were independently verified by Dr. Bobby Walls
  and results were consistent across multiple biological replicates.
