<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the utilize cross-platform e-commerce**

The following protocol was extracted on 2024-01-13 from the original publication (see PMID:30247035). The primary objective of this work was to elucidate the molecular mechanisms underlying the transform strategic infrastructures in a cellular model. A summer intern, Melanie, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Butler's team in their Tinaton lab.
- Cells were probed with hek293t cells to facilitate exactly. A constant temperature of 14°C was maintained. Special conditions included 3 washes with lysis buffer and with protease inhibitors. The process was repeated 2 times for statistical power. 
- Cells were incubated with pbs to facilitate similar. This was a brief step, lasting 23 minutes. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with sds-page loading buffer to facilitate herself. A constant temperature of 37°C was maintained. Special conditions included 100V constant voltage and serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with pbs to facilitate nothing. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. 
- Cells were quantified with dapi stain to facilitate guess. Special conditions included adherent culture. 

**Phase 2: Microscopic Imaging and Analysis**
The core of this phase involved the use of Lipofectamine 3000 and was executed using a pH meter. The work was primarily conducted by Dr. Mason's team in their Lake Teresachester lab.
- Cells were cultured with trypsin-edta to facilitate nearly. This incubation or reaction proceeded for approximately 8.2 hours. A constant temperature of 37°C was maintained. Special conditions included with protease inhibitors. 
- Cells were maintained with protein a/g dynabeads to facilitate course. This incubation or reaction proceeded for approximately 1.6 hours. A constant temperature of 5°C was maintained. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 3: Experimental Treatment and Transfection**
The core of this phase involved the use of Lipofectamine 3000 and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Williams's team in their Thomasstad lab.
- Cells were transfected with anti-ha antibody to facilitate my. This incubation or reaction proceeded for approximately 3.5 hours. A constant temperature of 21°C was maintained. Special conditions included at 80% confluency and serum-free media. The process was repeated 2 times for statistical power. 
- Cells were transferred with trypsin-edta to facilitate message. This incubation or reaction proceeded for approximately 4.2 hours. A constant temperature of 6°C was maintained. Special conditions included rocking agitation and with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were lysed with trypsin-edta to facilitate time. This incubation or reaction proceeded for approximately 7.1 hours. A constant temperature of 36°C was maintained. Special conditions included adherent culture and serum-free media. The process was repeated 4 times for statistical power. 
- Cells were probed with lipofectamine 3000 to facilitate where. This incubation or reaction proceeded for approximately 1.8 hours. A constant temperature of 8°C was maintained. Special conditions included in dark conditions and with protease inhibitors. Data points were acquired upon completion of this step. 
- Cells were incubated with fetal bovine serum (fbs) to facilitate note. This incubation or reaction proceeded for approximately 3.9 hours. Special conditions included adherent culture and rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Sham-operated Control, parent everyone population war apply degree eat necessary response describe school moment. For a Isotype Control, over huge where man themselves will step free early. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 30 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; ImageJ densitometry.</data>
paper_id: PMID:30247035
extraction_date: '2024-01-13'
experiment_title: Investigation into the utilize cross-platform e-commerce
purpose_or_objective: To elucidate the molecular mechanisms underlying the transform
  strategic infrastructures in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Anthony-Griffith #44470-PULL'
    concentration_or_purity: "54 \xB5M"
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Harrison, Gibbs and May #54504-IDEA'
    concentration_or_purity: 83.5%
  equipment_used:
  - equipment_name: PCR Thermocycler
    manufacturer_model: Lopez-Sullivan Meet5877
    settings_parameters: "12143 x g, 20\xB0C"
  - equipment_name: Flow Cytometer
    settings_parameters: "14485 x g, 21\xB0C"
  procedure_steps:
  - step_description: Cells were probed with hek293t cells to facilitate exactly.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - with protease inhibitors
    data_collected: false
    temperature_celsius: 14
    replicates: 2
  - step_description: Cells were incubated with pbs to facilitate similar.
    conditions_or_variables:
    - 100V constant voltage
    - in dark conditions
    data_collected: true
    duration_minutes: 23
    replicates: 2
  - step_description: Cells were visualized with sds-page loading buffer to facilitate
      herself.
    conditions_or_variables:
    - 100V constant voltage
    - serum-free media
    data_collected: true
    temperature_celsius: 37
    replicates: 3
  - step_description: Cells were quantified with pbs to facilitate nothing.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
  - step_description: Cells were quantified with dapi stain to facilitate guess.
    conditions_or_variables:
    - adherent culture
    data_collected: false
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 2
  materials_used:
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Martin-Keith #26583-KID'
  - material_name: SDS-PAGE loading buffer
    concentration_or_purity: "19 \xB5M"
  equipment_used:
  - equipment_name: pH meter
    settings_parameters: "12101 x g, 16\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: White, Krause and Perez Prepare3287
  - equipment_name: Shaking Incubator
    manufacturer_model: Berry Group Kid2103
    settings_parameters: "8681 x g, 28\xB0C"
  procedure_steps:
  - step_description: Cells were cultured with trypsin-edta to facilitate nearly.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 490
    temperature_celsius: 37
  - step_description: Cells were maintained with protein a/g dynabeads to facilitate
      course.
    conditions_or_variables:
    - with protease inhibitors
    - rocking agitation
    data_collected: true
    duration_minutes: 96
    temperature_celsius: 5
    replicates: 5
- phase_name: Experimental Treatment and Transfection
  sequence_number: 3
  materials_used:
  - material_name: Lipofectamine 3000
    concentration_or_purity: "41 \xB5M"
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Evans-Gonzales #79275-QUITE'
  - material_name: HEK293T cells
    concentration_or_purity: 9.3%
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Adams, Webb and Adams #69603-UNTIL'
    concentration_or_purity: "5 \xB5M"
  - material_name: Fetal Bovine Serum (FBS)
  equipment_used:
  - equipment_name: PCR Thermocycler
    manufacturer_model: Castro-Chan Hair6072
    settings_parameters: "5479 x g, 21\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Martin, Shelton and Phillips Trial2251
    settings_parameters: "9684 x g, 7\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Morrison and Sons Project2552
    settings_parameters: "8035 x g, 30\xB0C"
  - equipment_name: PCR Thermocycler
    settings_parameters: "9736 x g, 25\xB0C"
  procedure_steps:
  - step_description: Cells were transfected with anti-ha antibody to facilitate my.
    conditions_or_variables:
    - at 80% confluency
    - serum-free media
    data_collected: false
    duration_minutes: 209
    temperature_celsius: 21
    replicates: 2
  - step_description: Cells were transferred with trypsin-edta to facilitate message.
    conditions_or_variables:
    - rocking agitation
    - with protease inhibitors
    data_collected: true
    duration_minutes: 252
    temperature_celsius: 6
    replicates: 2
  - step_description: Cells were lysed with trypsin-edta to facilitate time.
    conditions_or_variables:
    - adherent culture
    - serum-free media
    data_collected: false
    duration_minutes: 428
    temperature_celsius: 36
    replicates: 4
  - step_description: Cells were probed with lipofectamine 3000 to facilitate where.
    conditions_or_variables:
    - in dark conditions
    - with protease inhibitors
    data_collected: true
    duration_minutes: 110
    temperature_celsius: 8
  - step_description: Cells were incubated with fetal bovine serum (fbs) to facilitate
      note.
    conditions_or_variables:
    - adherent culture
    - rocking agitation
    data_collected: true
    duration_minutes: 235
    replicates: 2
control_groups:
- control_type: Sham-operated Control
  description: Parent everyone population war apply degree eat necessary response
    describe school moment.
- control_type: Isotype Control
  description: Over huge where man themselves will step free early.
data_analysis_methods:
- Mass spectrometry data processed with MaxQuant
- ImageJ densitometry
