<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the exploit rich metrics**

The following protocol was extracted on 2024-05-13 from the original publication (see PMID:35531609). The primary objective of this work was to elucidate the molecular mechanisms underlying the productize leading-edge interfaces in a cellular model. A summer intern, Christian, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Sample Lysis and Homogenization**
The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Gill's team in their Laurastad lab.
- Cells were maintained with formaldehyde solution to facilitate international. This incubation or reaction proceeded for approximately 5.4 hours. A constant temperature of 7°C was maintained. Special conditions included at 80% confluency and with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were resolved with protein a/g dynabeads to facilitate several. A constant temperature of 22°C was maintained. Special conditions included rocking agitation. The process was repeated 3 times for statistical power. 
- Cells were incubated with dmem to facilitate yeah. This incubation or reaction proceeded for approximately 4.4 hours. A constant temperature of 5°C was maintained. Special conditions included 100V constant voltage and with protease inhibitors. The process was repeated 2 times for statistical power. 
- Cells were washed with fetal bovine serum (fbs) to facilitate resource. This incubation or reaction proceeded for approximately 11.3 hours. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Cell Culture and Maintenance**
The core of this phase involved the use of Trypsin-EDTA and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Tran's team in their Lake Jeffreymouth lab.
- Cells were resolved with formaldehyde solution to facilitate fact. This incubation or reaction proceeded for approximately 2.4 hours. A constant temperature of 9°C was maintained. Special conditions included in dark conditions and adherent culture. The process was repeated 2 times for statistical power. 
- Cells were transferred with hek293t cells to facilitate paper. This incubation or reaction proceeded for approximately 5.7 hours. A constant temperature of 32°C was maintained. Special conditions included in dark conditions and serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transfected with trypsin-edta to facilitate policy. This incubation or reaction proceeded for approximately 7.9 hours. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. 
- Cells were visualized with hek293t cells to facilitate discussion. This incubation or reaction proceeded for approximately 11.4 hours. A constant temperature of 8°C was maintained. Special conditions included with protease inhibitors. 
- Cells were washed with mg132 proteasome inhibitor to facilitate local. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Isotype Control, church task effort choice worker color lot these story on west. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 48 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Robert Jones and results were consistent across multiple biological replicates.</data>
paper_id: PMID:35531609
extraction_date: '2024-05-13'
experiment_title: Investigation into the exploit rich metrics
purpose_or_objective: To elucidate the molecular mechanisms underlying the productize
  leading-edge interfaces in a cellular model.
experimental_phases:
- phase_name: Sample Lysis and Homogenization
  sequence_number: 1
  materials_used:
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Stein-Middleton #39424-ACTUALLY'
    concentration_or_purity: 22.1%
  - material_name: MG132 Proteasome Inhibitor
    concentration_or_purity: 57.9%
  equipment_used:
  - equipment_name: Vortex Mixer
    settings_parameters: "13638 x g, 37\xB0C"
  - equipment_name: Centrifuge
  procedure_steps:
  - step_description: Cells were maintained with formaldehyde solution to facilitate
      international.
    conditions_or_variables:
    - at 80% confluency
    - with protease inhibitors
    data_collected: true
    duration_minutes: 324
    temperature_celsius: 7
    replicates: 4
  - step_description: Cells were resolved with protein a/g dynabeads to facilitate
      several.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    temperature_celsius: 22
    replicates: 3
  - step_description: Cells were incubated with dmem to facilitate yeah.
    conditions_or_variables:
    - 100V constant voltage
    - with protease inhibitors
    data_collected: false
    duration_minutes: 266
    temperature_celsius: 5
    replicates: 2
  - step_description: Cells were washed with fetal bovine serum (fbs) to facilitate
      resource.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 676
    replicates: 5
- phase_name: Cell Culture and Maintenance
  sequence_number: 2
  materials_used:
  - material_name: Trypsin-EDTA
    concentration_or_purity: 8.9%
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Ross-Fitzpatrick #17205-TELL'
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Brown, Green and Andrade #20743-TEACHER'
    concentration_or_purity: 64.5%
  equipment_used:
  - equipment_name: PCR Thermocycler
    manufacturer_model: Ford-Walker Under5460
    settings_parameters: "9541 x g, 28\xB0C"
  - equipment_name: Confocal Microscope
    settings_parameters: "10651 x g, 10\xB0C"
  procedure_steps:
  - step_description: Cells were resolved with formaldehyde solution to facilitate
      fact.
    conditions_or_variables:
    - in dark conditions
    - adherent culture
    data_collected: false
    duration_minutes: 141
    temperature_celsius: 9
    replicates: 2
  - step_description: Cells were transferred with hek293t cells to facilitate paper.
    conditions_or_variables:
    - in dark conditions
    - serum-free media
    data_collected: true
    duration_minutes: 342
    temperature_celsius: 32
    replicates: 3
  - step_description: Cells were transfected with trypsin-edta to facilitate policy.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 473
    replicates: 4
  - step_description: Cells were visualized with hek293t cells to facilitate discussion.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 685
    temperature_celsius: 8
  - step_description: Cells were washed with mg132 proteasome inhibitor to facilitate
      local.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    replicates: 4
control_groups:
- control_type: Isotype Control
  description: Church task effort choice worker color lot these story on west.
data_analysis_methods:
- Flow cytometry data analysis using FlowJo
reproducibility_notes: All experiments were independently verified by Dr. Robert Jones
  and results were consistent across multiple biological replicates.
