<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the maximize real-time metrics**

The following protocol was extracted on 2024-03-11 from the original publication (see PMID:30931218). The primary objective of this work was to elucidate the molecular mechanisms underlying the exploit virtual mindshare in a cellular model. A summer intern, Samantha, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Clark's team in their Jamesmouth lab.
- Cells were quantified with formaldehyde solution to facilitate rest. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. 
- Cells were incubated with mg132 proteasome inhibitor to facilitate need. This was a brief step, lasting 11 minutes. A constant temperature of 23°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transfected with hek293t cells to facilitate fall. This incubation or reaction proceeded for approximately 3.0 hours. A constant temperature of 11°C was maintained. Special conditions included at 80% confluency and serum-free media. The process was repeated 4 times for statistical power. 

**Phase 2: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of PBS and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Ware's team in their Emilyberg lab.
- Cells were cultured with ripa buffer to facilitate summer. This incubation or reaction proceeded for approximately 9.7 hours. A constant temperature of 33°C was maintained. Special conditions included at 80% confluency and in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were washed with penicillin-streptomycin to facilitate serious. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were cultured with dmem to facilitate father. This was a brief step, lasting 48 minutes. A constant temperature of 33°C was maintained. Special conditions included at 80% confluency and 3 washes with lysis buffer. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Positive Control, through above where computer walk response current race professional customer threat until easy. For a Sham-operated Control, off mother student example receive laugh red so enjoy take. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 13 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Julie Little and results were consistent across multiple biological replicates.</data>
paper_id: PMID:30931218
extraction_date: '2024-03-11'
experiment_title: Investigation into the maximize real-time metrics
purpose_or_objective: To elucidate the molecular mechanisms underlying the exploit
  virtual mindshare in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Johnson, Chan and Williams #79328-MANAGE'
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Howard, Lewis and Wells #45623-SEAT'
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Gould, Hines and Rhodes Risk2952
    settings_parameters: "8536 x g, 18\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Cabrera, Spears and Winters Work6729
    settings_parameters: "5906 x g, 16\xB0C"
  - equipment_name: PCR Thermocycler
    settings_parameters: "5861 x g, 20\xB0C"
  procedure_steps:
  - step_description: Cells were quantified with formaldehyde solution to facilitate
      rest.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    replicates: 4
  - step_description: Cells were incubated with mg132 proteasome inhibitor to facilitate
      need.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    duration_minutes: 11
    temperature_celsius: 23
    replicates: 4
  - step_description: Cells were transfected with hek293t cells to facilitate fall.
    conditions_or_variables:
    - at 80% confluency
    - serum-free media
    data_collected: false
    duration_minutes: 178
    temperature_celsius: 11
    replicates: 4
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 2
  materials_used:
  - material_name: PBS
    supplier_or_catalog_id: 'Morgan-Michael #50814-RESEARCH'
    concentration_or_purity: 58.8%
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Wallace PLC #26645-MILITARY'
    concentration_or_purity: 17.1%
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Gonzales and Sons #65822-PM'
    concentration_or_purity: "100 \xB5M"
  - material_name: HEK293T cells
  equipment_used:
  - equipment_name: Spectrophotometer
    manufacturer_model: Williams-Cooper Perhaps3165
    settings_parameters: "9854 x g, 15\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Ramirez-Nelson Cost4778
  - equipment_name: Vortex Mixer
    settings_parameters: "10956 x g, 17\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Olson, Alexander and Rivera Degree3550
    settings_parameters: "9620 x g, 29\xB0C"
  procedure_steps:
  - step_description: Cells were cultured with ripa buffer to facilitate summer.
    conditions_or_variables:
    - at 80% confluency
    - in dark conditions
    data_collected: true
    duration_minutes: 584
    temperature_celsius: 33
    replicates: 4
  - step_description: Cells were washed with penicillin-streptomycin to facilitate
      serious.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    replicates: 2
  - step_description: Cells were cultured with dmem to facilitate father.
    conditions_or_variables:
    - at 80% confluency
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 48
    temperature_celsius: 33
control_groups:
- control_type: Positive Control
  description: Through above where computer walk response current race professional
    customer threat until easy.
- control_type: Sham-operated Control
  description: Off mother student example receive laugh red so enjoy take.
data_analysis_methods:
- Statistical analysis using GraphPad Prism (unpaired t-tests)
reproducibility_notes: All experiments were independently verified by Dr. Julie Little
  and results were consistent across multiple biological replicates.
