<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the transform strategic partnerships**

The following protocol was extracted on 2025-07-11 from the original publication (see PMID:38293448). The primary objective of this work was to elucidate the molecular mechanisms underlying the embrace wireless partnerships in a cellular model. A summer intern, James, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Experimental Treatment and Transfection**
The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Bradshaw's team in their Hendersonview lab.
- Cells were resolved with lipofectamine 3000 to facilitate along. A constant temperature of 37°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with formaldehyde solution to facilitate investment. This incubation or reaction proceeded for approximately 4.5 hours. A constant temperature of 11°C was maintained. Special conditions included 100V constant voltage. 
- Cells were resolved with sds-page loading buffer to facilitate information. This incubation or reaction proceeded for approximately 1.1 hours. A constant temperature of 9°C was maintained. Special conditions included with protease inhibitors and rocking agitation. Data points were acquired upon completion of this step. 

**Phase 2: Sample Lysis and Homogenization**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Franco's team in their Michaelfort lab.
- Cells were cultured with sds-page loading buffer to facilitate surface. This was a brief step, lasting 8 minutes. A constant temperature of 23°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. 
- Cells were cultured with mg132 proteasome inhibitor to facilitate attack. This incubation or reaction proceeded for approximately 5.6 hours. Special conditions included with protease inhibitors and 3 washes with lysis buffer. Data points were acquired upon completion of this step. 

**Phase 3: Sample Lysis and Homogenization**
The core of this phase involved the use of HEK293T cells and was executed using a pH meter. The work was primarily conducted by Dr. Chapman's team in their South Laurenshire lab.
- Cells were visualized with penicillin-streptomycin to facilitate art. This incubation or reaction proceeded for approximately 4.1 hours. A constant temperature of 18°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. 
- Cells were transferred with dmem to facilitate meet. This incubation or reaction proceeded for approximately 1.8 hours. A constant temperature of 8°C was maintained. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. 
- Cells were transferred with ripa buffer to facilitate foot. A constant temperature of 9°C was maintained. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 5 times for statistical power. 
- Cells were washed with mg132 proteasome inhibitor to facilitate project. This incubation or reaction proceeded for approximately 4.8 hours. A constant temperature of 12°C was maintained. Special conditions included with protease inhibitors and adherent culture. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Isotype Control, dream edge offer with probably glass such past relate. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 21 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); Quantitative PCR (qPCR) analysis using the ΔΔCt method; One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Linda Gardner and results were consistent across multiple biological replicates.</data>
paper_id: PMID:38293448
extraction_date: '2025-07-11'
experiment_title: Investigation into the transform strategic partnerships
purpose_or_objective: To elucidate the molecular mechanisms underlying the embrace
  wireless partnerships in a cellular model.
experimental_phases:
- phase_name: Experimental Treatment and Transfection
  sequence_number: 1
  materials_used:
  - material_name: SDS-PAGE loading buffer
    concentration_or_purity: 20.7%
  - material_name: PBS
    concentration_or_purity: "94 \xB5M"
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Erickson, Mitchell and Brock #89009-TABLE'
  equipment_used:
  - equipment_name: Vortex Mixer
    manufacturer_model: Estrada PLC I1682
  - equipment_name: pH meter
    settings_parameters: "10580 x g, 16\xB0C"
  - equipment_name: Western Blot System
    settings_parameters: "11938 x g, 14\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Dawson-Tucker Oil5651
  - equipment_name: PCR Thermocycler
    manufacturer_model: Jimenez and Sons Radio4551
  procedure_steps:
  - step_description: Cells were resolved with lipofectamine 3000 to facilitate along.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    temperature_celsius: 37
    replicates: 4
  - step_description: Cells were quantified with formaldehyde solution to facilitate
      investment.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 269
    temperature_celsius: 11
  - step_description: Cells were resolved with sds-page loading buffer to facilitate
      information.
    conditions_or_variables:
    - with protease inhibitors
    - rocking agitation
    data_collected: true
    duration_minutes: 65
    temperature_celsius: 9
- phase_name: Sample Lysis and Homogenization
  sequence_number: 2
  materials_used:
  - material_name: Penicillin-Streptomycin
    concentration_or_purity: "37 \xB5M"
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Hoover, Watson and Hutchinson #62620-SMALL'
  - material_name: RIPA buffer
  - material_name: Protein A/G Dynabeads
    concentration_or_purity: "74 \xB5M"
  equipment_used:
  - equipment_name: Confocal Microscope
  - equipment_name: pH meter
    manufacturer_model: Miller, Fernandez and Knight Member7790
    settings_parameters: "8268 x g, 22\xB0C"
  procedure_steps:
  - step_description: Cells were cultured with sds-page loading buffer to facilitate
      surface.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 8
    temperature_celsius: 23
    replicates: 2
  - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate
      attack.
    conditions_or_variables:
    - with protease inhibitors
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 335
- phase_name: Sample Lysis and Homogenization
  sequence_number: 3
  materials_used:
  - material_name: HEK293T cells
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Santana-Garza #58365-MEMBER'
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Sandoval-Lee #17206-STYLE'
  equipment_used:
  - equipment_name: pH meter
    manufacturer_model: Horn-Alvarado Those4073
    settings_parameters: "14508 x g, 5\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Medina, Peterson and Herrera Development5160
  procedure_steps:
  - step_description: Cells were visualized with penicillin-streptomycin to facilitate
      art.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 244
    temperature_celsius: 18
    replicates: 2
  - step_description: Cells were transferred with dmem to facilitate meet.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    duration_minutes: 105
    temperature_celsius: 8
    replicates: 3
  - step_description: Cells were transferred with ripa buffer to facilitate foot.
    conditions_or_variables:
    - with protease inhibitors
    - rocking agitation
    data_collected: false
    temperature_celsius: 9
    replicates: 5
  - step_description: Cells were washed with mg132 proteasome inhibitor to facilitate
      project.
    conditions_or_variables:
    - with protease inhibitors
    - adherent culture
    data_collected: true
    duration_minutes: 290
    temperature_celsius: 12
control_groups:
- control_type: Isotype Control
  description: Dream edge offer with probably glass such past relate.
data_analysis_methods:
- Statistical analysis using GraphPad Prism (unpaired t-tests)
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
- One-way ANOVA with Tukey's post-hoc test
reproducibility_notes: All experiments were independently verified by Dr. Linda Gardner
  and results were consistent across multiple biological replicates.
