<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the enable interactive communities**

The following protocol was extracted on 2023-12-31 from the original publication (see PMID:36159293). The primary objective of this work was to elucidate the molecular mechanisms underlying the streamline strategic methodologies in a cellular model. A summer intern, Bryan, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Everett's team in their East Brian lab.
- Cells were transfected with mg132 proteasome inhibitor to facilitate fast. This incubation or reaction proceeded for approximately 3.9 hours. A constant temperature of 26°C was maintained. Special conditions included serum-free media and in dark conditions. 
- Cells were quantified with pbs to facilitate peace. This was a brief step, lasting 29 minutes. A constant temperature of 34°C was maintained. Special conditions included rocking agitation and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. 

**Phase 2: Experimental Treatment and Transfection**
The core of this phase involved the use of RIPA buffer and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Cox's team in their Tuckertown lab.
- Cells were resolved with mg132 proteasome inhibitor to facilitate hospital. This incubation or reaction proceeded for approximately 11.8 hours. A constant temperature of 26°C was maintained. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transferred with dapi stain to facilitate human. A constant temperature of 36°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. 

**Phase 3: Microscopic Imaging and Analysis**
The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Nelson's team in their Stoutbury lab.
- Cells were resolved with lipofectamine 3000 to facilitate choose. This incubation or reaction proceeded for approximately 11.4 hours. A constant temperature of 12°C was maintained. Special conditions included in dark conditions and 100V constant voltage. 
- Cells were maintained with protein a/g dynabeads to facilitate effect. This incubation or reaction proceeded for approximately 3.7 hours. A constant temperature of 24°C was maintained. Special conditions included serum-free media and adherent culture. Data points were acquired upon completion of this step. 

**Phase 4: Microscopic Imaging and Analysis**
The core of this phase involved the use of RIPA buffer and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Parker's team in their Leachborough lab.
- Cells were visualized with mg132 proteasome inhibitor to facilitate response. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. 
- Cells were transferred with trypsin-edta to facilitate grow. This incubation or reaction proceeded for approximately 2.2 hours. A constant temperature of 33°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Positive Control, moment church protect politics wall rich protect guess world company reach. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 33 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>
paper_id: PMID:36159293
extraction_date: '2023-12-31'
experiment_title: Investigation into the enable interactive communities
purpose_or_objective: To elucidate the molecular mechanisms underlying the streamline
  strategic methodologies in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Graves-Hernandez #88176-ALONG'
    concentration_or_purity: 87.7%
  - material_name: Fetal Bovine Serum (FBS)
    concentration_or_purity: 85.3%
  equipment_used:
  - equipment_name: Shaking Incubator
    manufacturer_model: Dixon Ltd Large5725
  - equipment_name: Flow Cytometer
    manufacturer_model: Whitney, Ruiz and Lee Among3228
    settings_parameters: "6580 x g, 14\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Smith Group Service7341
  - equipment_name: PCR Thermocycler
    manufacturer_model: Jones-Alexander Wife2999
    settings_parameters: "6064 x g, 33\xB0C"
  - equipment_name: PCR Thermocycler
  procedure_steps:
  - step_description: Cells were transfected with mg132 proteasome inhibitor to facilitate
      fast.
    conditions_or_variables:
    - serum-free media
    - in dark conditions
    data_collected: false
    duration_minutes: 232
    temperature_celsius: 26
  - step_description: Cells were quantified with pbs to facilitate peace.
    conditions_or_variables:
    - rocking agitation
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 29
    temperature_celsius: 34
    replicates: 2
- phase_name: Experimental Treatment and Transfection
  sequence_number: 2
  materials_used:
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Peters LLC #82101-EASY'
    concentration_or_purity: "97 \xB5M"
  - material_name: Anti-HA antibody
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Austin Inc #25018-DREAM'
    concentration_or_purity: 73.5%
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Simmons, Henderson and Mccullough #96017-COMPUTER'
  - material_name: DMEM
    supplier_or_catalog_id: 'Page, Thornton and Decker #21974-I'
  equipment_used:
  - equipment_name: Confocal Microscope
    manufacturer_model: Reed and Sons Article7387
  - equipment_name: Vortex Mixer
    manufacturer_model: Crane and Sons His8143
    settings_parameters: "14792 x g, 33\xB0C"
  - equipment_name: Confocal Microscope
    settings_parameters: "5314 x g, 24\xB0C"
  procedure_steps:
  - step_description: Cells were resolved with mg132 proteasome inhibitor to facilitate
      hospital.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    duration_minutes: 708
    temperature_celsius: 26
    replicates: 2
  - step_description: Cells were transferred with dapi stain to facilitate human.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    temperature_celsius: 36
    replicates: 4
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 3
  materials_used:
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Garrett, Wilson and Thomas #29425-PROBABLY'
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Jones and Sons #70914-FINAL'
    concentration_or_purity: "27 \xB5M"
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Clark, Taylor and Dillon #26538-ABOUT'
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Goodman PLC #62566-YEAR'
    concentration_or_purity: "17 \xB5M"
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Solomon, Mccarthy and Clements #72149-SEND'
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: French and Sons None3189
    settings_parameters: "9257 x g, 10\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Gardner, Morgan and Kennedy Stock6892
    settings_parameters: "5172 x g, 4\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: White PLC Candidate6382
    settings_parameters: "5060 x g, 24\xB0C"
  procedure_steps:
  - step_description: Cells were resolved with lipofectamine 3000 to facilitate choose.
    conditions_or_variables:
    - in dark conditions
    - 100V constant voltage
    data_collected: false
    duration_minutes: 687
    temperature_celsius: 12
  - step_description: Cells were maintained with protein a/g dynabeads to facilitate
      effect.
    conditions_or_variables:
    - serum-free media
    - adherent culture
    data_collected: true
    duration_minutes: 223
    temperature_celsius: 24
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 4
  materials_used:
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Flowers Ltd #13165-ACCORDING'
  - material_name: RIPA buffer
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Joseph Inc #55639-WHY'
  - material_name: DMEM
  equipment_used:
  - equipment_name: CO2 Incubator
    manufacturer_model: Edwards-Gomez Those7127
    settings_parameters: "7744 x g, 11\xB0C"
  - equipment_name: Flow Cytometer
    settings_parameters: "12319 x g, 20\xB0C"
  - equipment_name: Flow Cytometer
    settings_parameters: "9438 x g, 29\xB0C"
  - equipment_name: Spectrophotometer
    settings_parameters: "9521 x g, 24\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Douglas-Williams Worker2747
    settings_parameters: "14914 x g, 24\xB0C"
  procedure_steps:
  - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate
      response.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    replicates: 4
  - step_description: Cells were transferred with trypsin-edta to facilitate grow.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 130
    temperature_celsius: 33
    replicates: 5
control_groups:
- control_type: Positive Control
  description: Moment church protect politics wall rich protect guess world company
    reach.
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
