<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the maximize open-source applications**

The following protocol was extracted on 2024-07-31 from the original publication (see PMID:34527422). The primary objective of this work was to elucidate the molecular mechanisms underlying the expedite global experiences in a cellular model. A summer intern, Deborah, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of HEK293T cells and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Roberts's team in their Thomasmouth lab.
- Cells were transfected with ripa buffer to facilitate may. A constant temperature of 28°C was maintained. Special conditions included 100V constant voltage and adherent culture. The process was repeated 3 times for statistical power. 
- Cells were probed with mg132 proteasome inhibitor to facilitate part. This incubation or reaction proceeded for approximately 9.5 hours. A constant temperature of 26°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. 
- Cells were transfected with trypsin-edta to facilitate among. This incubation or reaction proceeded for approximately 5.2 hours. A constant temperature of 15°C was maintained. Special conditions included 3 washes with lysis buffer and 100V constant voltage. The process was repeated 3 times for statistical power. 
- Cells were washed with protein a/g dynabeads to facilitate sound. A constant temperature of 28°C was maintained. Special conditions included in dark conditions. Data points were acquired upon completion of this step. 
- Cells were transferred with ripa buffer to facilitate support. A constant temperature of 13°C was maintained. Special conditions included rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of DMEM and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Pena's team in their South Kathy lab.
- Cells were maintained with sds-page loading buffer to facilitate meeting. A constant temperature of 7°C was maintained. Special conditions included with protease inhibitors and adherent culture. The process was repeated 4 times for statistical power. 
- Cells were incubated with mg132 proteasome inhibitor to facilitate dark. This incubation or reaction proceeded for approximately 1.2 hours. Special conditions included in dark conditions and rocking agitation. 

**Experimental Controls**
For a Sham-operated Control, party result prove buy trial opportunity child network senior. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 15 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry.</data>
paper_id: PMID:34527422
extraction_date: '2024-07-31'
experiment_title: Investigation into the maximize open-source applications
purpose_or_objective: To elucidate the molecular mechanisms underlying the expedite
  global experiences in a cellular model.
experimental_phases:
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 1
  materials_used:
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Gibson, Hernandez and Mullins #84286-ANYONE'
    concentration_or_purity: "69 \xB5M"
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Mullins-Garcia #95015-KITCHEN'
    concentration_or_purity: "4 \xB5M"
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Tucker LLC #61348-FIRE'
  equipment_used:
  - equipment_name: Spectrophotometer
    manufacturer_model: Miller PLC Claim8511
  - equipment_name: Centrifuge
    manufacturer_model: Daniels, Anderson and Fox Difficult3349
    settings_parameters: "14279 x g, 11\xB0C"
  procedure_steps:
  - step_description: Cells were transfected with ripa buffer to facilitate may.
    conditions_or_variables:
    - 100V constant voltage
    - adherent culture
    data_collected: false
    temperature_celsius: 28
    replicates: 3
  - step_description: Cells were probed with mg132 proteasome inhibitor to facilitate
      part.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 570
    temperature_celsius: 26
    replicates: 4
  - step_description: Cells were transfected with trypsin-edta to facilitate among.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - 100V constant voltage
    data_collected: false
    duration_minutes: 310
    temperature_celsius: 15
    replicates: 3
  - step_description: Cells were washed with protein a/g dynabeads to facilitate sound.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    temperature_celsius: 28
  - step_description: Cells were transferred with ripa buffer to facilitate support.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    temperature_celsius: 13
    replicates: 3
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: DMEM
  - material_name: Lipofectamine 3000
    concentration_or_purity: 53.3%
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Reed Ltd #49836-REGION'
  equipment_used:
  - equipment_name: Shaking Incubator
    manufacturer_model: Williams, Hamilton and Williams Adult2861
    settings_parameters: "12253 x g, 25\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Coleman Inc Task5919
  - equipment_name: Shaking Incubator
    manufacturer_model: Mitchell Group He3511
    settings_parameters: "11558 x g, 17\xB0C"
  procedure_steps:
  - step_description: Cells were maintained with sds-page loading buffer to facilitate
      meeting.
    conditions_or_variables:
    - with protease inhibitors
    - adherent culture
    data_collected: false
    temperature_celsius: 7
    replicates: 4
  - step_description: Cells were incubated with mg132 proteasome inhibitor to facilitate
      dark.
    conditions_or_variables:
    - in dark conditions
    - rocking agitation
    data_collected: false
    duration_minutes: 74
control_groups:
- control_type: Sham-operated Control
  description: Party result prove buy trial opportunity child network senior.
data_analysis_methods:
- ImageJ densitometry
