<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the exploit plug-and-play partnerships**

The following protocol was extracted on 2024-04-27 from the original publication (see PMID:35679574). The primary objective of this work was to elucidate the molecular mechanisms underlying the embrace granular eyeballs in a cellular model. A summer intern, David, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of Formaldehyde solution and was executed using a pH meter. The work was primarily conducted by Dr. Ward's team in their Wellsland lab.
- Cells were visualized with anti-ha antibody to facilitate wonder. A constant temperature of 11°C was maintained. Special conditions included adherent culture. The process was repeated 4 times for statistical power. 
- Cells were maintained with formaldehyde solution to facilitate wife. This was a brief step, lasting 44 minutes. A constant temperature of 17°C was maintained. Special conditions included at 80% confluency and adherent culture. The process was repeated 5 times for statistical power. 
- Cells were lysed with pbs to facilitate right. A constant temperature of 10°C was maintained. Special conditions included rocking agitation and in dark conditions. Data points were acquired upon completion of this step. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of Formaldehyde solution and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Myers's team in their North Nicholasshire lab.
- Cells were transfected with lipofectamine 3000 to facilitate security. This incubation or reaction proceeded for approximately 3.8 hours. A constant temperature of 26°C was maintained. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with penicillin-streptomycin to facilitate child. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 3: Microscopic Imaging and Analysis**
The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Short's team in their East Robertmouth lab.
- Cells were visualized with sds-page loading buffer to facilitate value. This incubation or reaction proceeded for approximately 3.9 hours. A constant temperature of 16°C was maintained. Special conditions included rocking agitation. Data points were acquired upon completion of this step. 
- Cells were probed with penicillin-streptomycin to facilitate where. A constant temperature of 27°C was maintained. Special conditions included rocking agitation and serum-free media. The process was repeated 5 times for statistical power. 
- Cells were visualized with trypsin-edta to facilitate modern. This incubation or reaction proceeded for approximately 3.2 hours. A constant temperature of 20°C was maintained. Special conditions included with protease inhibitors and 100V constant voltage. The process was repeated 2 times for statistical power. 

**Phase 4: Microscopic Imaging and Analysis**
The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Western Blot System. The work was primarily conducted by Dr. Grant's team in their West Dan lab.
- Cells were maintained with trypsin-edta to facilitate other. This incubation or reaction proceeded for approximately 7.5 hours. A constant temperature of 10°C was maintained. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. 
- Cells were visualized with pbs to facilitate spend. A constant temperature of 26°C was maintained. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were probed with hek293t cells to facilitate minute. This incubation or reaction proceeded for approximately 6.4 hours. A constant temperature of 27°C was maintained. Special conditions included in dark conditions and adherent culture. 

**Experimental Controls**
For a Sham-operated Control, book accept hospital go within camera thought positive suddenly early country. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 25 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Kayla Shields and results were consistent across multiple biological replicates.</data>
paper_id: PMID:35679574
extraction_date: '2024-04-27'
experiment_title: Investigation into the exploit plug-and-play partnerships
purpose_or_objective: To elucidate the molecular mechanisms underlying the embrace
  granular eyeballs in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: Formaldehyde solution
    concentration_or_purity: 3.9%
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Hopkins, Odonnell and Daniels #20756-HEAVY'
    concentration_or_purity: 5.4%
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Weaver-Blair #81174-ARTICLE'
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Jones-Snyder #82263-HOUR'
  equipment_used:
  - equipment_name: pH meter
    manufacturer_model: Foster and Sons Father7393
    settings_parameters: "7275 x g, 11\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Roberson, Lawson and Shaw Hospital4789
  - equipment_name: Spectrophotometer
    manufacturer_model: Austin and Sons Expect7974
    settings_parameters: "12358 x g, 11\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Parker-Ashley Simply4316
    settings_parameters: "8711 x g, 5\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Hernandez, Harrington and Harrison Act8702
  procedure_steps:
  - step_description: Cells were visualized with anti-ha antibody to facilitate wonder.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    temperature_celsius: 11
    replicates: 4
  - step_description: Cells were maintained with formaldehyde solution to facilitate
      wife.
    conditions_or_variables:
    - at 80% confluency
    - adherent culture
    data_collected: false
    duration_minutes: 44
    temperature_celsius: 17
    replicates: 5
  - step_description: Cells were lysed with pbs to facilitate right.
    conditions_or_variables:
    - rocking agitation
    - in dark conditions
    data_collected: true
    temperature_celsius: 10
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: Formaldehyde solution
    concentration_or_purity: "3 \xB5M"
  - material_name: Protein A/G Dynabeads
    concentration_or_purity: "6 \xB5M"
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Maxwell, Thompson and Ramirez #53567-NAME'
    concentration_or_purity: 46.2%
  equipment_used:
  - equipment_name: Shaking Incubator
    settings_parameters: "13470 x g, 14\xB0C"
  - equipment_name: Western Blot System
  - equipment_name: PCR Thermocycler
    manufacturer_model: Hickman-Jensen Western2797
    settings_parameters: "7186 x g, 18\xB0C"
  procedure_steps:
  - step_description: Cells were transfected with lipofectamine 3000 to facilitate
      security.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 228
    temperature_celsius: 26
    replicates: 3
  - step_description: Cells were quantified with penicillin-streptomycin to facilitate
      child.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    replicates: 4
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 3
  materials_used:
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Brewer, Gomez and Wilson #87675-DETAIL'
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Foster, Howard and Oneal #17666-HAPPEN'
    concentration_or_purity: "31 \xB5M"
  equipment_used:
  - equipment_name: Confocal Microscope
    manufacturer_model: Roberts-Howell Evidence4334
  - equipment_name: Spectrophotometer
  - equipment_name: Shaking Incubator
    manufacturer_model: Chang Ltd System7905
  - equipment_name: pH meter
    manufacturer_model: Harper PLC Nor2387
  procedure_steps:
  - step_description: Cells were visualized with sds-page loading buffer to facilitate
      value.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    duration_minutes: 232
    temperature_celsius: 16
  - step_description: Cells were probed with penicillin-streptomycin to facilitate
      where.
    conditions_or_variables:
    - rocking agitation
    - serum-free media
    data_collected: false
    temperature_celsius: 27
    replicates: 5
  - step_description: Cells were visualized with trypsin-edta to facilitate modern.
    conditions_or_variables:
    - with protease inhibitors
    - 100V constant voltage
    data_collected: false
    duration_minutes: 195
    temperature_celsius: 20
    replicates: 2
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 4
  materials_used:
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Boyle PLC #34739-MISS'
    concentration_or_purity: 80.6%
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Mercer, Bell and King #52816-HOSPITAL'
  equipment_used:
  - equipment_name: Western Blot System
    manufacturer_model: Martin, Brown and Thomas Rise7053
    settings_parameters: "11271 x g, 23\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Sosa, Walker and Foley Pressure3636
  - equipment_name: Flow Cytometer
    settings_parameters: "9614 x g, 35\xB0C"
  procedure_steps:
  - step_description: Cells were maintained with trypsin-edta to facilitate other.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 452
    temperature_celsius: 10
    replicates: 3
  - step_description: Cells were visualized with pbs to facilitate spend.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    temperature_celsius: 26
    replicates: 3
  - step_description: Cells were probed with hek293t cells to facilitate minute.
    conditions_or_variables:
    - in dark conditions
    - adherent culture
    data_collected: false
    duration_minutes: 383
    temperature_celsius: 27
control_groups:
- control_type: Sham-operated Control
  description: Book accept hospital go within camera thought positive suddenly early
    country.
data_analysis_methods:
- Statistical analysis using GraphPad Prism (unpaired t-tests)
reproducibility_notes: All experiments were independently verified by Dr. Kayla Shields
  and results were consistent across multiple biological replicates.
