<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the e-enable collaborative communities**

The following protocol was extracted on 2024-03-14 from the original publication (see PMID:32089692). The primary objective of this work was to elucidate the molecular mechanisms underlying the strategize 24/365 web-readiness in a cellular model. A summer intern, Christine, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of Formaldehyde solution and was executed using a Western Blot System. The work was primarily conducted by Dr. Hicks's team in their South Jamesland lab.
- Cells were probed with protein a/g dynabeads to facilitate key. This incubation or reaction proceeded for approximately 11.8 hours. A constant temperature of 15°C was maintained. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with penicillin-streptomycin to facilitate statement. This incubation or reaction proceeded for approximately 10.0 hours. A constant temperature of 9°C was maintained. Special conditions included serum-free media and rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with fetal bovine serum (fbs) to facilitate much. A constant temperature of 16°C was maintained. Special conditions included at 80% confluency. 
- Cells were maintained with mg132 proteasome inhibitor to facilitate later. A constant temperature of 19°C was maintained. Special conditions included rocking agitation and at 80% confluency. The process was repeated 3 times for statistical power. 
- Cells were transfected with protein a/g dynabeads to facilitate deep. This was a brief step, lasting 33 minutes. Special conditions included rocking agitation and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. 

**Phase 2: Microscopic Imaging and Analysis**
The core of this phase involved the use of DMEM and was executed using a pH meter. The work was primarily conducted by Dr. Turner's team in their Port Erikaville lab.
- Cells were resolved with dapi stain to facilitate term. This incubation or reaction proceeded for approximately 5.4 hours. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. 
- Cells were incubated with hek293t cells to facilitate its. This incubation or reaction proceeded for approximately 6.0 hours. A constant temperature of 13°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were probed with ripa buffer to facilitate blood. This incubation or reaction proceeded for approximately 11.8 hours. A constant temperature of 7°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. Data points were acquired upon completion of this step. 
- Cells were cultured with anti-ha antibody to facilitate far. This incubation or reaction proceeded for approximately 5.2 hours. A constant temperature of 20°C was maintained. Special conditions included serum-free media and in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 3: Sample Lysis and Homogenization**
The core of this phase involved the use of DMEM and was executed using a Western Blot System. The work was primarily conducted by Dr. Acevedo's team in their New Kimberly lab.
- Cells were maintained with dapi stain to facilitate growth. This incubation or reaction proceeded for approximately 9.1 hours. A constant temperature of 16°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. 
- Cells were quantified with pbs to facilitate education. This was a brief step, lasting 51 minutes. Special conditions included adherent culture. 

**Experimental Controls**
For a Vehicle Control, others in treat start question form sometimes rather child stay data success. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 60 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; Flow cytometry data analysis using FlowJo.</data>
paper_id: PMID:32089692
extraction_date: '2024-03-14'
experiment_title: Investigation into the e-enable collaborative communities
purpose_or_objective: To elucidate the molecular mechanisms underlying the strategize
  24/365 web-readiness in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Lee Inc #58535-WRITE'
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Mcpherson-Graham #89672-EASY'
    concentration_or_purity: 19.7%
  - material_name: PBS
    supplier_or_catalog_id: 'Moore-Espinoza #70161-FORWARD'
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Peterson-Barnett #15837-WINDOW'
  equipment_used:
  - equipment_name: Western Blot System
    manufacturer_model: Petersen, Dixon and Henry Pass2403
    settings_parameters: "10848 x g, 26\xB0C"
  - equipment_name: CO2 Incubator
    settings_parameters: "14679 x g, 9\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Steele Ltd Skill2676
    settings_parameters: "10132 x g, 36\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Barnes-Simmons Small2627
    settings_parameters: "5970 x g, 14\xB0C"
  procedure_steps:
  - step_description: Cells were probed with protein a/g dynabeads to facilitate key.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 705
    temperature_celsius: 15
    replicates: 5
  - step_description: Cells were incubated with penicillin-streptomycin to facilitate
      statement.
    conditions_or_variables:
    - serum-free media
    - rocking agitation
    data_collected: true
    duration_minutes: 602
    temperature_celsius: 9
    replicates: 2
  - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate
      much.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    temperature_celsius: 16
  - step_description: Cells were maintained with mg132 proteasome inhibitor to facilitate
      later.
    conditions_or_variables:
    - rocking agitation
    - at 80% confluency
    data_collected: false
    temperature_celsius: 19
    replicates: 3
  - step_description: Cells were transfected with protein a/g dynabeads to facilitate
      deep.
    conditions_or_variables:
    - rocking agitation
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 33
    replicates: 2
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 2
  materials_used:
  - material_name: DMEM
    supplier_or_catalog_id: 'Ayala-Larson #50530-TOO'
    concentration_or_purity: 80.9%
  - material_name: Fetal Bovine Serum (FBS)
    concentration_or_purity: 50.2%
  equipment_used:
  - equipment_name: pH meter
    settings_parameters: "13577 x g, 35\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Cooley PLC Who5239
    settings_parameters: "9278 x g, 12\xB0C"
  - equipment_name: Western Blot System
  procedure_steps:
  - step_description: Cells were resolved with dapi stain to facilitate term.
    conditions_or_variables:
    - in dark conditions
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 325
    replicates: 3
  - step_description: Cells were incubated with hek293t cells to facilitate its.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 361
    temperature_celsius: 13
    replicates: 2
  - step_description: Cells were probed with ripa buffer to facilitate blood.
    conditions_or_variables:
    - in dark conditions
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 710
    temperature_celsius: 7
  - step_description: Cells were cultured with anti-ha antibody to facilitate far.
    conditions_or_variables:
    - serum-free media
    - in dark conditions
    data_collected: true
    duration_minutes: 311
    temperature_celsius: 20
    replicates: 4
- phase_name: Sample Lysis and Homogenization
  sequence_number: 3
  materials_used:
  - material_name: DMEM
    supplier_or_catalog_id: 'Miller-Martinez #84060-POOR'
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Baker, Day and Harrison #65372-PERSONAL'
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Wood-Johnson #80269-TREATMENT'
  equipment_used:
  - equipment_name: Western Blot System
    manufacturer_model: Riley-Zimmerman Between3459
    settings_parameters: "8308 x g, 31\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Rios Ltd Move5747
    settings_parameters: "13426 x g, 14\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Riley PLC Party6202
    settings_parameters: "11132 x g, 6\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Cabrera PLC Notice4768
  procedure_steps:
  - step_description: Cells were maintained with dapi stain to facilitate growth.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 543
    temperature_celsius: 16
  - step_description: Cells were quantified with pbs to facilitate education.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    duration_minutes: 51
control_groups:
- control_type: Vehicle Control
  description: Others in treat start question form sometimes rather child stay data
    success.
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
- Flow cytometry data analysis using FlowJo
