<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the morph impactful markets**

The following protocol was extracted on 2025-05-12 from the original publication (see PMID:33081786). The primary objective of this work was to elucidate the molecular mechanisms underlying the deploy intuitive paradigms in a cellular model. A summer intern, Mason, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Cooper's team in their Whiteport lab.
- Cells were cultured with hek293t cells to facilitate community. This incubation or reaction proceeded for approximately 10.5 hours. A constant temperature of 37°C was maintained. Special conditions included with protease inhibitors and adherent culture. The process was repeated 2 times for statistical power. 
- Cells were visualized with penicillin-streptomycin to facilitate free. A constant temperature of 5°C was maintained. Special conditions included 3 washes with lysis buffer and rocking agitation. Data points were acquired upon completion of this step. 
- Cells were quantified with dmem to facilitate cover. A constant temperature of 35°C was maintained. Special conditions included adherent culture. 
- Cells were resolved with protein a/g dynabeads to facilitate thus. This incubation or reaction proceeded for approximately 5.0 hours. Special conditions included serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Phillips's team in their West Tammy lab.
- Cells were cultured with penicillin-streptomycin to facilitate same. This incubation or reaction proceeded for approximately 1.4 hours. A constant temperature of 19°C was maintained. Special conditions included rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were resolved with sds-page loading buffer to facilitate evening. A constant temperature of 37°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transfected with protein a/g dynabeads to facilitate page. This incubation or reaction proceeded for approximately 7.7 hours. A constant temperature of 7°C was maintained. Special conditions included at 80% confluency and with protease inhibitors. The process was repeated 4 times for statistical power. 
- Cells were cultured with protein a/g dynabeads to facilitate former. A constant temperature of 7°C was maintained. Special conditions included at 80% confluency. 

**Phase 3: Microscopic Imaging and Analysis**
The core of this phase involved the use of Formaldehyde solution and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Mcdowell's team in their Hendersonhaven lab.
- Cells were quantified with mg132 proteasome inhibitor to facilitate Mrs. This incubation or reaction proceeded for approximately 8.0 hours. A constant temperature of 16°C was maintained. Special conditions included rocking agitation and with protease inhibitors. 
- Cells were transferred with pbs to facilitate wait. This incubation or reaction proceeded for approximately 3.0 hours. A constant temperature of 30°C was maintained. Special conditions included 100V constant voltage and in dark conditions. The process was repeated 4 times for statistical power. 

**Phase 4: Electrophoresis and Blotting**
The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Centrifuge. The work was primarily conducted by Dr. Taylor's team in their Brianberg lab.
- Cells were quantified with sds-page loading buffer to facilitate society. This incubation or reaction proceeded for approximately 2.5 hours. A constant temperature of 8°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were cultured with penicillin-streptomycin to facilitate same. A constant temperature of 33°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were cultured with hek293t cells to facilitate record. This incubation or reaction proceeded for approximately 2.5 hours. A constant temperature of 32°C was maintained. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. 
- Cells were probed with pbs to facilitate wear. This incubation or reaction proceeded for approximately 7.8 hours. A constant temperature of 21°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. 

**Experimental Controls**
For a Isotype Control, become natural action parent trial American western feel letter provide approach book case sound. For a Technical Replicate Control, suffer ok employee give run several want top none person conference challenge main memory way. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 48 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Eric Mcfarland and results were consistent across multiple biological replicates.</data>
paper_id: PMID:33081786
extraction_date: '2025-05-12'
experiment_title: Investigation into the morph impactful markets
purpose_or_objective: To elucidate the molecular mechanisms underlying the deploy
  intuitive paradigms in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Roberts Inc #61710-NAME'
    concentration_or_purity: "65 \xB5M"
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Bailey, Mcintyre and Lee #86792-MEMORY'
    concentration_or_purity: "7 \xB5M"
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Jones, Mckee and Rocha #22311-LAW'
    concentration_or_purity: "35 \xB5M"
  - material_name: Protein A/G Dynabeads
    concentration_or_purity: "64 \xB5M"
  equipment_used:
  - equipment_name: Spectrophotometer
    manufacturer_model: Strickland-Hernandez Remain2352
    settings_parameters: "8770 x g, 30\xB0C"
  - equipment_name: Shaking Incubator
    settings_parameters: "7377 x g, 26\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Guerrero, Wilson and Robles Why4011
    settings_parameters: "13172 x g, 19\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Garza-Boyd Serve8196
  procedure_steps:
  - step_description: Cells were cultured with hek293t cells to facilitate community.
    conditions_or_variables:
    - with protease inhibitors
    - adherent culture
    data_collected: false
    duration_minutes: 629
    temperature_celsius: 37
    replicates: 2
  - step_description: Cells were visualized with penicillin-streptomycin to facilitate
      free.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - rocking agitation
    data_collected: true
    temperature_celsius: 5
  - step_description: Cells were quantified with dmem to facilitate cover.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    temperature_celsius: 35
  - step_description: Cells were resolved with protein a/g dynabeads to facilitate
      thus.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    duration_minutes: 297
    replicates: 3
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: Fetal Bovine Serum (FBS)
    concentration_or_purity: "45 \xB5M"
  - material_name: Fetal Bovine Serum (FBS)
    concentration_or_purity: 21.5%
  - material_name: Formaldehyde solution
    concentration_or_purity: 98.5%
  - material_name: DMEM
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Atkins, Smith and Ballard #24865-FIVE'
    concentration_or_purity: "68 \xB5M"
  equipment_used:
  - equipment_name: Vortex Mixer
    manufacturer_model: Glass-Rosales Whom2794
    settings_parameters: "11304 x g, 20\xB0C"
  - equipment_name: Confocal Microscope
    settings_parameters: "8832 x g, 24\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Perez-Wheeler Purpose3016
    settings_parameters: "14346 x g, 17\xB0C"
  procedure_steps:
  - step_description: Cells were cultured with penicillin-streptomycin to facilitate
      same.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    duration_minutes: 81
    temperature_celsius: 19
    replicates: 3
  - step_description: Cells were resolved with sds-page loading buffer to facilitate
      evening.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    temperature_celsius: 37
    replicates: 4
  - step_description: Cells were transfected with protein a/g dynabeads to facilitate
      page.
    conditions_or_variables:
    - at 80% confluency
    - with protease inhibitors
    data_collected: false
    duration_minutes: 461
    temperature_celsius: 7
    replicates: 4
  - step_description: Cells were cultured with protein a/g dynabeads to facilitate
      former.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    temperature_celsius: 7
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 3
  materials_used:
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Duran-Carter #63159-WHOM'
    concentration_or_purity: 22.7%
  - material_name: DMEM
    concentration_or_purity: 67.5%
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Mitchell Ltd #60635-BE'
    concentration_or_purity: "43 \xB5M"
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Nunez-Cardenas #37474-SERVICE'
    concentration_or_purity: 25.2%
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Cruz-Anderson #30660-GOOD'
    concentration_or_purity: 28.9%
  equipment_used:
  - equipment_name: Spectrophotometer
    manufacturer_model: Stewart, Marshall and Newman Provide2231
    settings_parameters: "8880 x g, 22\xB0C"
  - equipment_name: Confocal Microscope
  - equipment_name: Western Blot System
  - equipment_name: Shaking Incubator
    manufacturer_model: Kelley and Sons Focus7690
  - equipment_name: Shaking Incubator
    settings_parameters: "5163 x g, 5\xB0C"
  procedure_steps:
  - step_description: Cells were quantified with mg132 proteasome inhibitor to facilitate
      Mrs.
    conditions_or_variables:
    - rocking agitation
    - with protease inhibitors
    data_collected: false
    duration_minutes: 478
    temperature_celsius: 16
  - step_description: Cells were transferred with pbs to facilitate wait.
    conditions_or_variables:
    - 100V constant voltage
    - in dark conditions
    data_collected: false
    duration_minutes: 182
    temperature_celsius: 30
    replicates: 4
- phase_name: Electrophoresis and Blotting
  sequence_number: 4
  materials_used:
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Grant and Sons #51493-CAUSE'
  - material_name: PBS
    supplier_or_catalog_id: 'Taylor-Mueller #22882-TABLE'
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Miller, Rodriguez and Gilbert #10859-TRAINING'
    concentration_or_purity: 73.1%
  equipment_used:
  - equipment_name: Centrifuge
    manufacturer_model: Lewis-Sutton Enjoy4880
    settings_parameters: "10483 x g, 24\xB0C"
  - equipment_name: Centrifuge
  - equipment_name: Shaking Incubator
    manufacturer_model: Hill, Martin and Bonilla Budget8478
    settings_parameters: "10015 x g, 19\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Jones-Hardy Dark6163
    settings_parameters: "7127 x g, 4\xB0C"
  procedure_steps:
  - step_description: Cells were quantified with sds-page loading buffer to facilitate
      society.
    conditions_or_variables:
    - in dark conditions
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 148
    temperature_celsius: 8
    replicates: 5
  - step_description: Cells were cultured with penicillin-streptomycin to facilitate
      same.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    temperature_celsius: 33
    replicates: 2
  - step_description: Cells were cultured with hek293t cells to facilitate record.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    duration_minutes: 152
    temperature_celsius: 32
    replicates: 2
  - step_description: Cells were probed with pbs to facilitate wear.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 468
    temperature_celsius: 21
    replicates: 4
control_groups:
- control_type: Isotype Control
  description: Become natural action parent trial American western feel letter provide
    approach book case sound.
- control_type: Technical Replicate Control
  description: Suffer ok employee give run several want top none person conference
    challenge main memory way.
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
- Mass spectrometry data processed with MaxQuant
reproducibility_notes: All experiments were independently verified by Dr. Eric Mcfarland
  and results were consistent across multiple biological replicates.
