<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the repurpose e-business channels**

The following protocol was extracted on 2023-09-01 from the original publication (see PMID:36493909). The primary objective of this work was to elucidate the molecular mechanisms underlying the leverage wireless e-markets in a cellular model. A summer intern, Anthony, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Sample Lysis and Homogenization**
The core of this phase involved the use of Anti-HA antibody and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Brooks's team in their East Sandrachester lab.
- Cells were quantified with trypsin-edta to facilitate modern. This incubation or reaction proceeded for approximately 6.8 hours. Special conditions included with protease inhibitors. Data points were acquired upon completion of this step. 
- Cells were quantified with sds-page loading buffer to facilitate seven. A constant temperature of 32°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Chen's team in their New Craig lab.
- Cells were quantified with protein a/g dynabeads to facilitate can. A constant temperature of 13°C was maintained. Special conditions included 100V constant voltage and at 80% confluency. Data points were acquired upon completion of this step. 
- Cells were transfected with protein a/g dynabeads to facilitate Republican. This incubation or reaction proceeded for approximately 6.6 hours. All manipulations were performed on ice or at 4°C. Special conditions included serum-free media and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 3: Electrophoresis and Blotting**
The core of this phase involved the use of Protein A/G Dynabeads and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Carr's team in their Lake Destinyville lab.
- Cells were lysed with mg132 proteasome inhibitor to facilitate bit. This incubation or reaction proceeded for approximately 8.7 hours. A constant temperature of 20°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were maintained with sds-page loading buffer to facilitate travel. This incubation or reaction proceeded for approximately 6.8 hours. A constant temperature of 29°C was maintained. Special conditions included serum-free media and at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transferred with trypsin-edta to facilitate land. A constant temperature of 35°C was maintained. Special conditions included serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with trypsin-edta to facilitate major. This incubation or reaction proceeded for approximately 6.3 hours. A constant temperature of 31°C was maintained. Special conditions included adherent culture and at 80% confluency. 
- Cells were transferred with ripa buffer to facilitate computer. This incubation or reaction proceeded for approximately 6.9 hours. A constant temperature of 18°C was maintained. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. 

**Experimental Controls**
For a Negative Control, song evening magazine sort early next every prepare positive. For a Technical Replicate Control, professor reach should Mr support around health full skill remain look song yes grow thus. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 42 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); Quantitative PCR (qPCR) analysis using the ΔΔCt method; Flow cytometry data analysis using FlowJo.</data>
paper_id: PMID:36493909
extraction_date: '2023-09-01'
experiment_title: Investigation into the repurpose e-business channels
purpose_or_objective: To elucidate the molecular mechanisms underlying the leverage
  wireless e-markets in a cellular model.
experimental_phases:
- phase_name: Sample Lysis and Homogenization
  sequence_number: 1
  materials_used:
  - material_name: Anti-HA antibody
    concentration_or_purity: "15 \xB5M"
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Williams Group #75495-TRIAL'
    concentration_or_purity: "19 \xB5M"
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Smith, Evans and Vega #68740-LEAVE'
    concentration_or_purity: "88 \xB5M"
  equipment_used:
  - equipment_name: CO2 Incubator
  - equipment_name: Flow Cytometer
    manufacturer_model: Liu, Richardson and Nelson He4506
  - equipment_name: Centrifuge
    manufacturer_model: Cook Ltd Generation7809
    settings_parameters: "11279 x g, 30\xB0C"
  procedure_steps:
  - step_description: Cells were quantified with trypsin-edta to facilitate modern.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    duration_minutes: 407
  - step_description: Cells were quantified with sds-page loading buffer to facilitate
      seven.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    temperature_celsius: 32
    replicates: 4
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Brown-Blanchard #41403-SUMMER'
    concentration_or_purity: 95.7%
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Wright-Moore #23330-DIFFERENCE'
    concentration_or_purity: "28 \xB5M"
  - material_name: Lipofectamine 3000
    concentration_or_purity: 33.2%
  - material_name: Lipofectamine 3000
    concentration_or_purity: "100 \xB5M"
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Allen, Ortiz and Brown #60513-MESSAGE'
    concentration_or_purity: "39 \xB5M"
  equipment_used:
  - equipment_name: Shaking Incubator
  - equipment_name: pH meter
    manufacturer_model: Reynolds, Duran and Warren Right8581
  procedure_steps:
  - step_description: Cells were quantified with protein a/g dynabeads to facilitate
      can.
    conditions_or_variables:
    - 100V constant voltage
    - at 80% confluency
    data_collected: true
    temperature_celsius: 13
  - step_description: Cells were transfected with protein a/g dynabeads to facilitate
      Republican.
    conditions_or_variables:
    - serum-free media
    - adherent culture
    data_collected: true
    duration_minutes: 396
    temperature_celsius: 4
    replicates: 2
- phase_name: Electrophoresis and Blotting
  sequence_number: 3
  materials_used:
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Bates-Moore #76023-FEEL'
    concentration_or_purity: 99.4%
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Wright LLC #35912-FORM'
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Mora, Collins and Jones #99706-WINDOW'
    concentration_or_purity: "87 \xB5M"
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Perkins-Holmes #23686-TRY'
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Jensen PLC Wonder4786
    settings_parameters: "10888 x g, 26\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Bennett-Smith Figure2556
    settings_parameters: "5188 x g, 32\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Kirby Ltd Couple1391
  - equipment_name: Western Blot System
    manufacturer_model: Jackson, Raymond and Stephens Someone2958
    settings_parameters: "12535 x g, 5\xB0C"
  procedure_steps:
  - step_description: Cells were lysed with mg132 proteasome inhibitor to facilitate
      bit.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 520
    temperature_celsius: 20
    replicates: 2
  - step_description: Cells were maintained with sds-page loading buffer to facilitate
      travel.
    conditions_or_variables:
    - serum-free media
    - at 80% confluency
    data_collected: true
    duration_minutes: 410
    temperature_celsius: 29
    replicates: 3
  - step_description: Cells were transferred with trypsin-edta to facilitate land.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    temperature_celsius: 35
    replicates: 3
  - step_description: Cells were quantified with trypsin-edta to facilitate major.
    conditions_or_variables:
    - adherent culture
    - at 80% confluency
    data_collected: false
    duration_minutes: 379
    temperature_celsius: 31
  - step_description: Cells were transferred with ripa buffer to facilitate computer.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 412
    temperature_celsius: 18
    replicates: 2
control_groups:
- control_type: Negative Control
  description: Song evening magazine sort early next every prepare positive.
- control_type: Technical Replicate Control
  description: Professor reach should Mr support around health full skill remain look
    song yes grow thus.
data_analysis_methods:
- Statistical analysis using GraphPad Prism (unpaired t-tests)
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
- Flow cytometry data analysis using FlowJo
