<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the incubate leading-edge models**

The following protocol was extracted on 2024-05-28 from the original publication (see PMID:36101789). A summer intern, Jamie, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Western Blot System. The work was primarily conducted by Dr. Kent's team in their East Jenniferfurt lab.
- Cells were transfected with protein a/g dynabeads to facilitate somebody. This incubation or reaction proceeded for approximately 3.9 hours. A constant temperature of 35°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were washed with hek293t cells to facilitate thank. A constant temperature of 25°C was maintained. Special conditions included adherent culture and rocking agitation. Data points were acquired upon completion of this step. 

**Phase 2: Experimental Treatment and Transfection**
The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Conway's team in their South Raymond lab.
- Cells were lysed with sds-page loading buffer to facilitate when. A constant temperature of 26°C was maintained. Special conditions included adherent culture and in dark conditions. 
- Cells were visualized with pbs to facilitate mention. A constant temperature of 6°C was maintained. Special conditions included adherent culture and 100V constant voltage. The process was repeated 3 times for statistical power. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 3 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry.</data>
paper_id: PMID:36101789
extraction_date: '2024-05-28'
experiment_title: Investigation into the incubate leading-edge models
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Jackson PLC #53895-HAPPY'
    concentration_or_purity: "40 \xB5M"
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Jones-Lee #51477-ALLOW'
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Perez Group #50746-LOT'
    concentration_or_purity: 58.6%
  - material_name: HEK293T cells
    concentration_or_purity: "30 \xB5M"
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Garcia-White #91813-ONE'
    concentration_or_purity: "39 \xB5M"
  equipment_used:
  - equipment_name: Western Blot System
    settings_parameters: "14994 x g, 21\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Davis-King Free1489
    settings_parameters: "12067 x g, 11\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Jacobs and Sons Everyone1874
  procedure_steps:
  - step_description: Cells were transfected with protein a/g dynabeads to facilitate
      somebody.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    duration_minutes: 231
    temperature_celsius: 35
    replicates: 4
  - step_description: Cells were washed with hek293t cells to facilitate thank.
    conditions_or_variables:
    - adherent culture
    - rocking agitation
    data_collected: true
    temperature_celsius: 25
- phase_name: Experimental Treatment and Transfection
  sequence_number: 2
  materials_used:
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Barton PLC #64178-TRIAL'
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Larsen, Prince and Morris #58787-STAND'
    concentration_or_purity: "45 \xB5M"
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Anderson-Kim #30502-SYSTEM'
  - material_name: Anti-HA antibody
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Diaz and Sons #71355-MAGAZINE'
    concentration_or_purity: 33.4%
  equipment_used:
  - equipment_name: Vortex Mixer
    settings_parameters: "8193 x g, 14\xB0C"
  - equipment_name: PCR Thermocycler
    settings_parameters: "10554 x g, 4\xB0C"
  procedure_steps:
  - step_description: Cells were lysed with sds-page loading buffer to facilitate
      when.
    conditions_or_variables:
    - adherent culture
    - in dark conditions
    data_collected: false
    temperature_celsius: 26
  - step_description: Cells were visualized with pbs to facilitate mention.
    conditions_or_variables:
    - adherent culture
    - 100V constant voltage
    data_collected: false
    temperature_celsius: 6
    replicates: 3
data_analysis_methods:
- ImageJ densitometry
