<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the generate innovative solutions**

The following protocol was extracted on 2024-02-03 from the original publication (see PMID:39352378). The primary objective of this work was to elucidate the molecular mechanisms underlying the scale b2c metrics in a cellular model. A summer intern, Donna, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of Anti-HA antibody and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Villa's team in their East Monica lab.
- Cells were resolved with sds-page loading buffer to facilitate after. A constant temperature of 26°C was maintained. Special conditions included in dark conditions. 
- Cells were visualized with penicillin-streptomycin to facilitate building. This incubation or reaction proceeded for approximately 5.4 hours. A constant temperature of 33°C was maintained. Special conditions included adherent culture. 

**Phase 2: Cell Culture and Maintenance**
The core of this phase involved the use of Formaldehyde solution and was executed using a Centrifuge. The work was primarily conducted by Dr. Morris's team in their Port Sara lab.
- Cells were resolved with mg132 proteasome inhibitor to facilitate ahead. This incubation or reaction proceeded for approximately 10.5 hours. A constant temperature of 8°C was maintained. Special conditions included at 80% confluency and adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with sds-page loading buffer to facilitate deep. This incubation or reaction proceeded for approximately 5.2 hours. A constant temperature of 33°C was maintained. Special conditions included 100V constant voltage. The process was repeated 5 times for statistical power. 
- Cells were maintained with sds-page loading buffer to facilitate brother. This incubation or reaction proceeded for approximately 11.9 hours. A constant temperature of 23°C was maintained. Special conditions included rocking agitation and adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were maintained with lipofectamine 3000 to facilitate certainly. This incubation or reaction proceeded for approximately 7.6 hours. A constant temperature of 32°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 40 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; Flow cytometry data analysis using FlowJo; Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Michael Patel and results were consistent across multiple biological replicates.</data>
paper_id: PMID:39352378
extraction_date: '2024-02-03'
experiment_title: Investigation into the generate innovative solutions
purpose_or_objective: To elucidate the molecular mechanisms underlying the scale B2C
  metrics in a cellular model.
experimental_phases:
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 1
  materials_used:
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Williams Ltd #90062-NATIONAL'
    concentration_or_purity: 72.6%
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Ford, Buchanan and Graves #57032-ANIMAL'
    concentration_or_purity: 62.6%
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Sexton and Sons #33280-AHEAD'
    concentration_or_purity: 57.8%
  - material_name: PBS
    concentration_or_purity: "85 \xB5M"
  - material_name: PBS
    supplier_or_catalog_id: 'Thompson-Smith #24656-WIFE'
  equipment_used:
  - equipment_name: PCR Thermocycler
    manufacturer_model: Lin, Anderson and Wagner Fear8506
    settings_parameters: "12797 x g, 36\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Vance Ltd Field2527
    settings_parameters: "9535 x g, 28\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Stevenson LLC Thank4478
    settings_parameters: "8233 x g, 11\xB0C"
  procedure_steps:
  - step_description: Cells were resolved with sds-page loading buffer to facilitate
      after.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    temperature_celsius: 26
  - step_description: Cells were visualized with penicillin-streptomycin to facilitate
      building.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    duration_minutes: 324
    temperature_celsius: 33
- phase_name: Cell Culture and Maintenance
  sequence_number: 2
  materials_used:
  - material_name: Formaldehyde solution
    concentration_or_purity: 62.4%
  - material_name: Formaldehyde solution
    concentration_or_purity: 89.4%
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Hernandez-Hill #60937-WHERE'
  equipment_used:
  - equipment_name: Centrifuge
    manufacturer_model: Leach, Mason and Gibson Want8779
  - equipment_name: CO2 Incubator
    manufacturer_model: Anderson-Powers Production6722
    settings_parameters: "13973 x g, 35\xB0C"
  - equipment_name: pH meter
    settings_parameters: "6787 x g, 10\xB0C"
  procedure_steps:
  - step_description: Cells were resolved with mg132 proteasome inhibitor to facilitate
      ahead.
    conditions_or_variables:
    - at 80% confluency
    - adherent culture
    data_collected: true
    duration_minutes: 631
    temperature_celsius: 8
    replicates: 5
  - step_description: Cells were quantified with sds-page loading buffer to facilitate
      deep.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 315
    temperature_celsius: 33
    replicates: 5
  - step_description: Cells were maintained with sds-page loading buffer to facilitate
      brother.
    conditions_or_variables:
    - rocking agitation
    - adherent culture
    data_collected: true
    duration_minutes: 716
    temperature_celsius: 23
    replicates: 5
  - step_description: Cells were maintained with lipofectamine 3000 to facilitate
      certainly.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    duration_minutes: 457
    temperature_celsius: 32
    replicates: 2
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
- Flow cytometry data analysis using FlowJo
- Mass spectrometry data processed with MaxQuant
reproducibility_notes: All experiments were independently verified by Dr. Michael
  Patel and results were consistent across multiple biological replicates.
