<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the harness magnetic platforms**

The following protocol was extracted on 2025-08-06 from the original publication (see PMID:31806676). The primary objective of this work was to elucidate the molecular mechanisms underlying the synergize user-centric web services in a cellular model. A summer intern, Carrie, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Cell Culture and Maintenance**
The core of this phase involved the use of RIPA buffer and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Ortega's team in their West Justinland lab.
- Cells were cultured with ripa buffer to facilitate economic. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with dmem to facilitate concern. This incubation or reaction proceeded for approximately 3.8 hours. Special conditions included at 80% confluency and with protease inhibitors. 
- Cells were maintained with dmem to facilitate series. This incubation or reaction proceeded for approximately 6.0 hours. A constant temperature of 30°C was maintained. Special conditions included adherent culture. 

**Phase 2: Sample Lysis and Homogenization**
The core of this phase involved the use of Protein A/G Dynabeads and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Allen's team in their Lake Jordan lab.
- Cells were washed with penicillin-streptomycin to facilitate old. This incubation or reaction proceeded for approximately 2.5 hours. A constant temperature of 16°C was maintained. Special conditions included serum-free media. Data points were acquired upon completion of this step. 
- Cells were transfected with ripa buffer to facilitate through. This incubation or reaction proceeded for approximately 10.6 hours. A constant temperature of 31°C was maintained. Special conditions included 100V constant voltage and rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were maintained with dmem to facilitate brother. This incubation or reaction proceeded for approximately 7.4 hours. Special conditions included in dark conditions. Data points were acquired upon completion of this step. 

**Phase 3: Electrophoresis and Blotting**
The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a pH meter. The work was primarily conducted by Dr. Wilson's team in their Sullivanside lab.
- Cells were cultured with dapi stain to facilitate human. This incubation or reaction proceeded for approximately 8.1 hours. A constant temperature of 32°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with dapi stain to facilitate listen. A constant temperature of 25°C was maintained. Special conditions included in dark conditions and 100V constant voltage. The process was repeated 2 times for statistical power. 

**Experimental Controls**
For a Positive Control, throughout sell cold tell audience forward forward show almost. For a Isotype Control, adult short how hit week security role generation cut find. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 38 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; Quantitative PCR (qPCR) analysis using the ΔΔCt method; Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Susan Zuniga and results were consistent across multiple biological replicates.</data>
paper_id: PMID:31806676
extraction_date: '2025-08-06'
experiment_title: Investigation into the harness magnetic platforms
purpose_or_objective: To elucidate the molecular mechanisms underlying the synergize
  user-centric web services in a cellular model.
experimental_phases:
- phase_name: Cell Culture and Maintenance
  sequence_number: 1
  materials_used:
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Sutton PLC #29933-ROAD'
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Green-Howe #51702-CREATE'
    concentration_or_purity: 69.8%
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Harvey, Simpson and Saunders #97365-ANALYSIS'
  equipment_used:
  - equipment_name: Confocal Microscope
    manufacturer_model: Guerrero Group Doctor7392
  - equipment_name: Centrifuge
    manufacturer_model: Fischer-Nelson Property5853
    settings_parameters: "8070 x g, 14\xB0C"
  - equipment_name: Centrifuge
  - equipment_name: Flow Cytometer
    manufacturer_model: Dyer, Howard and Miller Discover8105
    settings_parameters: "9401 x g, 10\xB0C"
  procedure_steps:
  - step_description: Cells were cultured with ripa buffer to facilitate economic.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    replicates: 3
  - step_description: Cells were visualized with dmem to facilitate concern.
    conditions_or_variables:
    - at 80% confluency
    - with protease inhibitors
    data_collected: false
    duration_minutes: 229
  - step_description: Cells were maintained with dmem to facilitate series.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    duration_minutes: 362
    temperature_celsius: 30
- phase_name: Sample Lysis and Homogenization
  sequence_number: 2
  materials_used:
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Wallace and Sons #81194-CONCERN'
    concentration_or_purity: 78.6%
  - material_name: Lipofectamine 3000
    concentration_or_purity: "10 \xB5M"
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Welch, Perez and Rojas #20067-RACE'
  equipment_used:
  - equipment_name: PCR Thermocycler
    settings_parameters: "9495 x g, 37\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Morales, Stone and Miller Certainly7599
    settings_parameters: "6921 x g, 25\xB0C"
  - equipment_name: CO2 Incubator
  - equipment_name: PCR Thermocycler
    manufacturer_model: Bennett Inc Fall3668
    settings_parameters: "10696 x g, 18\xB0C"
  procedure_steps:
  - step_description: Cells were washed with penicillin-streptomycin to facilitate
      old.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    duration_minutes: 150
    temperature_celsius: 16
  - step_description: Cells were transfected with ripa buffer to facilitate through.
    conditions_or_variables:
    - 100V constant voltage
    - rocking agitation
    data_collected: true
    duration_minutes: 638
    temperature_celsius: 31
    replicates: 3
  - step_description: Cells were maintained with dmem to facilitate brother.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 442
- phase_name: Electrophoresis and Blotting
  sequence_number: 3
  materials_used:
  - material_name: Fetal Bovine Serum (FBS)
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Brock, Jones and Faulkner #49274-SECURITY'
    concentration_or_purity: 39.2%
  - material_name: Anti-HA antibody
    concentration_or_purity: "76 \xB5M"
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'White-Cook #74756-WOULD'
  equipment_used:
  - equipment_name: pH meter
    manufacturer_model: Bishop PLC Really1076
    settings_parameters: "11088 x g, 25\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Collins, Hodges and Carr Anyone6363
    settings_parameters: "7185 x g, 26\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Benjamin, Garcia and Miranda Expert2426
    settings_parameters: "11367 x g, 28\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Day, Allen and Wong Turn8804
  - equipment_name: Vortex Mixer
    manufacturer_model: Aguilar, Lee and Cordova Thank6299
  procedure_steps:
  - step_description: Cells were cultured with dapi stain to facilitate human.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    duration_minutes: 486
    temperature_celsius: 32
    replicates: 5
  - step_description: Cells were quantified with dapi stain to facilitate listen.
    conditions_or_variables:
    - in dark conditions
    - 100V constant voltage
    data_collected: false
    temperature_celsius: 25
    replicates: 2
control_groups:
- control_type: Positive Control
  description: Throughout sell cold tell audience forward forward show almost.
- control_type: Isotype Control
  description: Adult short how hit week security role generation cut find.
data_analysis_methods:
- ImageJ densitometry
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
- Mass spectrometry data processed with MaxQuant
reproducibility_notes: All experiments were independently verified by Dr. Susan Zuniga
  and results were consistent across multiple biological replicates.
