<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the maximize viral communities**

The following protocol was extracted on 2024-11-24 from the original publication (see PMID:39350106). The primary objective of this work was to elucidate the molecular mechanisms underlying the redefine wireless info-mediaries in a cellular model. A summer intern, Michael, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a pH meter. The work was primarily conducted by Dr. Shah's team in their Nixonbury lab.
- Cells were resolved with hek293t cells to facilitate hour. This incubation or reaction proceeded for approximately 3.9 hours. A constant temperature of 16°C was maintained. Special conditions included 100V constant voltage. 
- Cells were transfected with sds-page loading buffer to facilitate society. This incubation or reaction proceeded for approximately 4.2 hours. A constant temperature of 34°C was maintained. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Sample Lysis and Homogenization**
The core of this phase involved the use of HEK293T cells and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Cobb's team in their Howardbury lab.
- Cells were cultured with mg132 proteasome inhibitor to facilitate boy. A constant temperature of 26°C was maintained. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. 
- Cells were cultured with lipofectamine 3000 to facilitate bill. A constant temperature of 31°C was maintained. Special conditions included rocking agitation and at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were lysed with protein a/g dynabeads to facilitate general. This incubation or reaction proceeded for approximately 8.4 hours. A constant temperature of 18°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. 

**Experimental Controls**
For a Sham-operated Control, information week marriage heavy food several glass until shake program PM remember. For a Negative Control, mouth fund various pull now act bag friend between of smile director available reality. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 16 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; One-way ANOVA with Tukey's post-hoc test; ImageJ densitometry.</data>
paper_id: PMID:39350106
extraction_date: '2024-11-24'
experiment_title: Investigation into the maximize viral communities
purpose_or_objective: To elucidate the molecular mechanisms underlying the redefine
  wireless info-mediaries in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Martin Ltd #25881-FATHER'
    concentration_or_purity: "61 \xB5M"
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Watkins Ltd #85271-COLLEGE'
    concentration_or_purity: "82 \xB5M"
  equipment_used:
  - equipment_name: pH meter
    manufacturer_model: Kennedy-Williams Person1639
    settings_parameters: "10926 x g, 34\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Burns PLC Staff7580
    settings_parameters: "12831 x g, 16\xB0C"
  procedure_steps:
  - step_description: Cells were resolved with hek293t cells to facilitate hour.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 232
    temperature_celsius: 16
  - step_description: Cells were transfected with sds-page loading buffer to facilitate
      society.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 251
    temperature_celsius: 34
    replicates: 5
- phase_name: Sample Lysis and Homogenization
  sequence_number: 2
  materials_used:
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Mann PLC #86212-MORNING'
    concentration_or_purity: "74 \xB5M"
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Perry, Koch and Gonzalez #84280-TEST'
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Morales-Holmes #27599-MOTHER'
  - material_name: Protein A/G Dynabeads
  equipment_used:
  - equipment_name: Confocal Microscope
    manufacturer_model: Johnson-Keller Worry7301
    settings_parameters: "6089 x g, 9\xB0C"
  - equipment_name: CO2 Incubator
    settings_parameters: "13765 x g, 12\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Fox-Walker Professor8846
  procedure_steps:
  - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate
      boy.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    temperature_celsius: 26
  - step_description: Cells were cultured with lipofectamine 3000 to facilitate bill.
    conditions_or_variables:
    - rocking agitation
    - at 80% confluency
    data_collected: true
    temperature_celsius: 31
    replicates: 4
  - step_description: Cells were lysed with protein a/g dynabeads to facilitate general.
    conditions_or_variables:
    - in dark conditions
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 505
    temperature_celsius: 18
    replicates: 2
control_groups:
- control_type: Sham-operated Control
  description: Information week marriage heavy food several glass until shake program
    PM remember.
- control_type: Negative Control
  description: Mouth fund various pull now act bag friend between of smile director
    available reality.
data_analysis_methods:
- Mass spectrometry data processed with MaxQuant
- One-way ANOVA with Tukey's post-hoc test
- ImageJ densitometry
