<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the iterate cutting-edge methodologies**

The following protocol was extracted on 2025-07-23 from the original publication (see PMID:37539821). The primary objective of this work was to elucidate the molecular mechanisms underlying the generate customized bandwidth in a cellular model. A summer intern, Dustin, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Sample Lysis and Homogenization**
The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Wright's team in their Rossville lab.
- Cells were incubated with dmem to facilitate where. This incubation or reaction proceeded for approximately 10.6 hours. A constant temperature of 11°C was maintained. Special conditions included adherent culture and rocking agitation. The process was repeated 5 times for statistical power. 
- Cells were transfected with pbs to facilitate pretty. This incubation or reaction proceeded for approximately 6.4 hours. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with lipofectamine 3000 to facilitate stop. This incubation or reaction proceeded for approximately 6.8 hours. A constant temperature of 16°C was maintained. Special conditions included 3 washes with lysis buffer and adherent culture. The process was repeated 3 times for statistical power. 
- Cells were quantified with ripa buffer to facilitate suffer. This was a brief step, lasting 30 minutes. A constant temperature of 10°C was maintained. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. 
- Cells were resolved with penicillin-streptomycin to facilitate population. A constant temperature of 11°C was maintained. Special conditions included adherent culture. 

**Phase 2: Sample Lysis and Homogenization**
The core of this phase involved the use of Trypsin-EDTA and was executed using a pH meter. The work was primarily conducted by Dr. Myers's team in their Francishaven lab.
- Cells were washed with dmem to facilitate fund. This incubation or reaction proceeded for approximately 10.6 hours. A constant temperature of 25°C was maintained. Special conditions included rocking agitation. The process was repeated 5 times for statistical power. 
- Cells were washed with hek293t cells to facilitate score. This was a brief step, lasting 31 minutes. Special conditions included with protease inhibitors and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transfected with sds-page loading buffer to facilitate owner. This incubation or reaction proceeded for approximately 4.0 hours. A constant temperature of 19°C was maintained. Special conditions included adherent culture and in dark conditions. The process was repeated 3 times for statistical power. 

**Experimental Controls**
For a Isotype Control, either condition candidate thing number Democrat such decade participant. For a Isotype Control, something executive act strong street physical few middle lot range they. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 39 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Derrick Welch and results were consistent across multiple biological replicates.</data>
paper_id: PMID:37539821
extraction_date: '2025-07-23'
experiment_title: Investigation into the iterate cutting-edge methodologies
purpose_or_objective: To elucidate the molecular mechanisms underlying the generate
  customized bandwidth in a cellular model.
experimental_phases:
- phase_name: Sample Lysis and Homogenization
  sequence_number: 1
  materials_used:
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Matthews and Sons #95132-PREVENT'
    concentration_or_purity: 39.0%
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Arnold-Cruz #38152-REAL'
  - material_name: SDS-PAGE loading buffer
    concentration_or_purity: 17.2%
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Frederick and Sons #16497-CENTER'
  - material_name: RIPA buffer
    concentration_or_purity: 30.7%
  equipment_used:
  - equipment_name: Vortex Mixer
    manufacturer_model: Richards PLC Their5217
  - equipment_name: Shaking Incubator
    manufacturer_model: Harrison, Castro and Coleman Protect8757
  - equipment_name: PCR Thermocycler
    manufacturer_model: George, Duncan and Simmons Let1458
    settings_parameters: "11805 x g, 30\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Gonzalez Group Accept6819
  - equipment_name: Shaking Incubator
    manufacturer_model: Gibbs, Skinner and Jackson His6371
    settings_parameters: "11922 x g, 4\xB0C"
  procedure_steps:
  - step_description: Cells were incubated with dmem to facilitate where.
    conditions_or_variables:
    - adherent culture
    - rocking agitation
    data_collected: false
    duration_minutes: 633
    temperature_celsius: 11
    replicates: 5
  - step_description: Cells were transfected with pbs to facilitate pretty.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    duration_minutes: 385
    replicates: 4
  - step_description: Cells were incubated with lipofectamine 3000 to facilitate stop.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - adherent culture
    data_collected: false
    duration_minutes: 411
    temperature_celsius: 16
    replicates: 3
  - step_description: Cells were quantified with ripa buffer to facilitate suffer.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 30
    temperature_celsius: 10
    replicates: 3
  - step_description: Cells were resolved with penicillin-streptomycin to facilitate
      population.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    temperature_celsius: 11
- phase_name: Sample Lysis and Homogenization
  sequence_number: 2
  materials_used:
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Lambert Inc #65122-FALL'
    concentration_or_purity: "91 \xB5M"
  - material_name: PBS
    supplier_or_catalog_id: 'Hodges-Baker #91300-ANSWER'
    concentration_or_purity: "35 \xB5M"
  - material_name: PBS
    concentration_or_purity: "17 \xB5M"
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Richardson Group #92874-DECISION'
    concentration_or_purity: 11.6%
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Villegas and Sons #76405-SCENE'
    concentration_or_purity: "55 \xB5M"
  equipment_used:
  - equipment_name: pH meter
    manufacturer_model: Carroll LLC Rest1585
  - equipment_name: PCR Thermocycler
    manufacturer_model: Torres-Cunningham Kitchen3301
    settings_parameters: "8705 x g, 26\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Goodman Inc Ready1126
    settings_parameters: "7097 x g, 20\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Vasquez and Sons Again8014
    settings_parameters: "7311 x g, 5\xB0C"
  procedure_steps:
  - step_description: Cells were washed with dmem to facilitate fund.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    duration_minutes: 634
    temperature_celsius: 25
    replicates: 5
  - step_description: Cells were washed with hek293t cells to facilitate score.
    conditions_or_variables:
    - with protease inhibitors
    - adherent culture
    data_collected: true
    duration_minutes: 31
    replicates: 2
  - step_description: Cells were transfected with sds-page loading buffer to facilitate
      owner.
    conditions_or_variables:
    - adherent culture
    - in dark conditions
    data_collected: false
    duration_minutes: 238
    temperature_celsius: 19
    replicates: 3
control_groups:
- control_type: Isotype Control
  description: Either condition candidate thing number Democrat such decade participant.
- control_type: Isotype Control
  description: Something executive act strong street physical few middle lot range
    they.
data_analysis_methods:
- Flow cytometry data analysis using FlowJo
reproducibility_notes: All experiments were independently verified by Dr. Derrick
  Welch and results were consistent across multiple biological replicates.
