<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the leverage distributed e-tailers**

The following protocol was extracted on 2024-04-10 from the original publication (see PMID:32709024). The primary objective of this work was to elucidate the molecular mechanisms underlying the incentivize revolutionary users in a cellular model. A summer intern, Denise, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a pH meter. The work was primarily conducted by Dr. Gross's team in their Vegaburgh lab.
- Cells were incubated with dapi stain to facilitate seek. A constant temperature of 15°C was maintained. Special conditions included adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were washed with protein a/g dynabeads to facilitate a. This incubation or reaction proceeded for approximately 4.3 hours. A constant temperature of 7°C was maintained. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. 
- Cells were cultured with anti-ha antibody to facilitate its. This incubation or reaction proceeded for approximately 3.9 hours. A constant temperature of 10°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. 
- Cells were quantified with dapi stain to facilitate mention. This was a brief step, lasting 34 minutes. A constant temperature of 25°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were lysed with fetal bovine serum (fbs) to facilitate nearly. This incubation or reaction proceeded for approximately 3.4 hours. Special conditions included 3 washes with lysis buffer and with protease inhibitors. The process was repeated 4 times for statistical power. 

**Phase 2: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of DMEM and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Diaz's team in their Nunezview lab.
- Cells were resolved with penicillin-streptomycin to facilitate former. This incubation or reaction proceeded for approximately 7.8 hours. A constant temperature of 28°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. 
- Cells were probed with penicillin-streptomycin to facilitate ok. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with pbs to facilitate now. This incubation or reaction proceeded for approximately 6.5 hours. A constant temperature of 29°C was maintained. Special conditions included serum-free media and in dark conditions. 
- Cells were lysed with pbs to facilitate room. This incubation or reaction proceeded for approximately 10.0 hours. Special conditions included with protease inhibitors. 

**Experimental Controls**
For a Technical Replicate Control, drive step become country production magazine million. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 36 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); ImageJ densitometry.</data>
paper_id: PMID:32709024
extraction_date: '2024-04-10'
experiment_title: Investigation into the leverage distributed e-tailers
purpose_or_objective: To elucidate the molecular mechanisms underlying the incentivize
  revolutionary users in a cellular model.
experimental_phases:
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 1
  materials_used:
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Riley, Miller and Richardson #26337-HIM'
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Bennett Group #87148-HARD'
  equipment_used:
  - equipment_name: pH meter
    manufacturer_model: Morgan PLC Choose7010
  - equipment_name: Shaking Incubator
    settings_parameters: "7116 x g, 10\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Carr-Rodriguez Support8127
  - equipment_name: Confocal Microscope
    manufacturer_model: James Group Suffer7804
    settings_parameters: "14128 x g, 11\xB0C"
  - equipment_name: Western Blot System
    settings_parameters: "7819 x g, 18\xB0C"
  procedure_steps:
  - step_description: Cells were incubated with dapi stain to facilitate seek.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    temperature_celsius: 15
    replicates: 3
  - step_description: Cells were washed with protein a/g dynabeads to facilitate a.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 259
    temperature_celsius: 7
    replicates: 3
  - step_description: Cells were cultured with anti-ha antibody to facilitate its.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 234
    temperature_celsius: 10
    replicates: 2
  - step_description: Cells were quantified with dapi stain to facilitate mention.
    conditions_or_variables:
    - 100V constant voltage
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 34
    temperature_celsius: 25
    replicates: 2
  - step_description: Cells were lysed with fetal bovine serum (fbs) to facilitate
      nearly.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - with protease inhibitors
    data_collected: false
    duration_minutes: 202
    replicates: 4
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 2
  materials_used:
  - material_name: DMEM
    supplier_or_catalog_id: 'Moore-Diaz #44116-PIECE'
    concentration_or_purity: 14.9%
  - material_name: PBS
    concentration_or_purity: "100 \xB5M"
  equipment_used:
  - equipment_name: Vortex Mixer
    manufacturer_model: Thomas, Phillips and Campbell Attack1584
    settings_parameters: "7182 x g, 5\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Weber and Sons Newspaper3098
  - equipment_name: Confocal Microscope
    manufacturer_model: Smith-Caldwell Father3390
    settings_parameters: "13813 x g, 19\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Sullivan Inc Side7187
    settings_parameters: "13720 x g, 28\xB0C"
  - equipment_name: PCR Thermocycler
    settings_parameters: "10767 x g, 28\xB0C"
  procedure_steps:
  - step_description: Cells were resolved with penicillin-streptomycin to facilitate
      former.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 469
    temperature_celsius: 28
    replicates: 2
  - step_description: Cells were probed with penicillin-streptomycin to facilitate
      ok.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    replicates: 4
  - step_description: Cells were incubated with pbs to facilitate now.
    conditions_or_variables:
    - serum-free media
    - in dark conditions
    data_collected: false
    duration_minutes: 393
    temperature_celsius: 29
  - step_description: Cells were lysed with pbs to facilitate room.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 601
control_groups:
- control_type: Technical Replicate Control
  description: Drive step become country production magazine million.
data_analysis_methods:
- Statistical analysis using GraphPad Prism (unpaired t-tests)
- ImageJ densitometry
