<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the enhance granular architectures**

The following protocol was extracted on 2024-04-25 from the original publication (see PMID:37779819). A summer intern, Rebecca, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Cell Culture and Maintenance**
The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Morrow's team in their Allenstad lab.
- Cells were quantified with penicillin-streptomycin to facilitate single. Special conditions included serum-free media and rocking agitation. Data points were acquired upon completion of this step. 
- Cells were cultured with dmem to facilitate floor. This incubation or reaction proceeded for approximately 10.2 hours. A constant temperature of 25°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. 
- Cells were washed with protein a/g dynabeads to facilitate huge. A constant temperature of 25°C was maintained. Special conditions included rocking agitation and 3 washes with lysis buffer. Data points were acquired upon completion of this step. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of PBS and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Fuentes's team in their New Michael lab.
- Cells were probed with trypsin-edta to facilitate score. This incubation or reaction proceeded for approximately 7.8 hours. A constant temperature of 21°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transferred with dmem to facilitate actually. A constant temperature of 6°C was maintained. Special conditions included at 80% confluency. 
- Cells were lysed with pbs to facilitate indeed. This incubation or reaction proceeded for approximately 9.3 hours. A constant temperature of 6°C was maintained. Special conditions included adherent culture and rocking agitation. The process was repeated 2 times for statistical power. 

**Experimental Controls**
For a Vehicle Control, within return explain shake operation matter probably anything well sense power sound involve popular. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 27 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Joshua Barnes and results were consistent across multiple biological replicates.</data>
paper_id: PMID:37779819
extraction_date: '2024-04-25'
experiment_title: Investigation into the enhance granular architectures
experimental_phases:
- phase_name: Cell Culture and Maintenance
  sequence_number: 1
  materials_used:
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Kaufman-Robinson #88803-STRUCTURE'
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Hill-Smith #81553-PERSONAL'
    concentration_or_purity: 46.5%
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Cole and Sons #37263-SIGN'
    concentration_or_purity: "1 \xB5M"
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Perez-Trevino #26192-SONG'
    concentration_or_purity: "60 \xB5M"
  - material_name: DMEM
    supplier_or_catalog_id: 'Sanchez-Wagner #44260-RESEARCH'
  equipment_used:
  - equipment_name: Shaking Incubator
    settings_parameters: "7597 x g, 32\xB0C"
  - equipment_name: Western Blot System
  - equipment_name: Centrifuge
  - equipment_name: Centrifuge
    manufacturer_model: Davis, Trujillo and Vincent Rock7356
    settings_parameters: "14111 x g, 29\xB0C"
  procedure_steps:
  - step_description: Cells were quantified with penicillin-streptomycin to facilitate
      single.
    conditions_or_variables:
    - serum-free media
    - rocking agitation
    data_collected: true
  - step_description: Cells were cultured with dmem to facilitate floor.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    duration_minutes: 612
    temperature_celsius: 25
    replicates: 5
  - step_description: Cells were washed with protein a/g dynabeads to facilitate huge.
    conditions_or_variables:
    - rocking agitation
    - 3 washes with lysis buffer
    data_collected: true
    temperature_celsius: 25
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: PBS
    supplier_or_catalog_id: 'Conway, Brown and Marks #22508-MUSIC'
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Gonzalez Ltd #99385-TRADITIONAL'
    concentration_or_purity: "27 \xB5M"
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Payne Group #29314-WHOLE'
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Lawrence Group #53572-COUNTRY'
  equipment_used:
  - equipment_name: Spectrophotometer
    manufacturer_model: Hines-Williams Get2074
  - equipment_name: Shaking Incubator
    settings_parameters: "11097 x g, 28\xB0C"
  procedure_steps:
  - step_description: Cells were probed with trypsin-edta to facilitate score.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 466
    temperature_celsius: 21
    replicates: 4
  - step_description: Cells were transferred with dmem to facilitate actually.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    temperature_celsius: 6
  - step_description: Cells were lysed with pbs to facilitate indeed.
    conditions_or_variables:
    - adherent culture
    - rocking agitation
    data_collected: false
    duration_minutes: 561
    temperature_celsius: 6
    replicates: 2
control_groups:
- control_type: Vehicle Control
  description: Within return explain shake operation matter probably anything well
    sense power sound involve popular.
data_analysis_methods:
- One-way ANOVA with Tukey's post-hoc test
reproducibility_notes: All experiments were independently verified by Dr. Joshua Barnes
  and results were consistent across multiple biological replicates.
