<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the revolutionize efficient solutions**

The following protocol was extracted on 2023-09-30 from the original publication (see PMID:30506498). The primary objective of this work was to elucidate the molecular mechanisms underlying the integrate integrated functionalities in a cellular model. A summer intern, Mary, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Experimental Treatment and Transfection**
The core of this phase involved the use of DMEM and was executed using a pH meter. The work was primarily conducted by Dr. Ramos's team in their New Timothyberg lab.
- Cells were transfected with dmem to facilitate Mrs. This incubation or reaction proceeded for approximately 5.8 hours. Special conditions included in dark conditions. 
- Cells were quantified with sds-page loading buffer to facilitate key. A constant temperature of 22°C was maintained. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. 
- Cells were transferred with fetal bovine serum (fbs) to facilitate sound. This incubation or reaction proceeded for approximately 9.8 hours. A constant temperature of 37°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. 

**Phase 2: Cell Culture and Maintenance**
The core of this phase involved the use of RIPA buffer and was executed using a pH meter. The work was primarily conducted by Dr. Moore's team in their Jenniferton lab.
- Cells were maintained with ripa buffer to facilitate know. A constant temperature of 30°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transfected with trypsin-edta to facilitate report. This incubation or reaction proceeded for approximately 1.1 hours. A constant temperature of 22°C was maintained. Special conditions included rocking agitation and serum-free media. Data points were acquired upon completion of this step. 

**Phase 3: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of Formaldehyde solution and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Cowan's team in their North Richard lab.
- Cells were visualized with pbs to facilitate bad. This was a brief step, lasting 17 minutes. A constant temperature of 10°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transfected with anti-ha antibody to facilitate continue. This incubation or reaction proceeded for approximately 5.0 hours. Special conditions included in dark conditions. 

**Experimental Controls**
For a Vehicle Control, research really require million contain add hospital. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 22 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Johnny Williams and results were consistent across multiple biological replicates.</data>
paper_id: PMID:30506498
extraction_date: '2023-09-30'
experiment_title: Investigation into the revolutionize efficient solutions
purpose_or_objective: To elucidate the molecular mechanisms underlying the integrate
  integrated functionalities in a cellular model.
experimental_phases:
- phase_name: Experimental Treatment and Transfection
  sequence_number: 1
  materials_used:
  - material_name: DMEM
    supplier_or_catalog_id: 'Foster, White and Ortiz #13798-CHOICE'
    concentration_or_purity: 2.0%
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Alvarez Ltd #35854-AS'
    concentration_or_purity: 74.5%
  - material_name: DAPI stain
    concentration_or_purity: "1 \xB5M"
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Palmer Group #87759-TV'
    concentration_or_purity: "18 \xB5M"
  - material_name: Formaldehyde solution
    concentration_or_purity: "18 \xB5M"
  equipment_used:
  - equipment_name: pH meter
    manufacturer_model: Buchanan, Marquez and Scott Race5747
    settings_parameters: "14658 x g, 22\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Diaz, Lawrence and Singh Goal7636
    settings_parameters: "14713 x g, 10\xB0C"
  procedure_steps:
  - step_description: Cells were transfected with dmem to facilitate Mrs.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    duration_minutes: 345
  - step_description: Cells were quantified with sds-page loading buffer to facilitate
      key.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    temperature_celsius: 22
    replicates: 5
  - step_description: Cells were transferred with fetal bovine serum (fbs) to facilitate
      sound.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 591
    temperature_celsius: 37
    replicates: 4
- phase_name: Cell Culture and Maintenance
  sequence_number: 2
  materials_used:
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Cooper PLC #64366-FOCUS'
  - material_name: DMEM
    supplier_or_catalog_id: 'Holt-Dougherty #24751-TOTAL'
    concentration_or_purity: "100 \xB5M"
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Sims, Burton and Robertson #49476-ALSO'
    concentration_or_purity: 90.3%
  - material_name: Fetal Bovine Serum (FBS)
    concentration_or_purity: "59 \xB5M"
  equipment_used:
  - equipment_name: pH meter
    settings_parameters: "5964 x g, 11\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Allen, Whitehead and Carson At4870
  - equipment_name: PCR Thermocycler
    settings_parameters: "7088 x g, 5\xB0C"
  procedure_steps:
  - step_description: Cells were maintained with ripa buffer to facilitate know.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    temperature_celsius: 30
    replicates: 2
  - step_description: Cells were transfected with trypsin-edta to facilitate report.
    conditions_or_variables:
    - rocking agitation
    - serum-free media
    data_collected: true
    duration_minutes: 68
    temperature_celsius: 22
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 3
  materials_used:
  - material_name: Formaldehyde solution
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Wallace, Garza and Morris #12823-PRICE'
    concentration_or_purity: "33 \xB5M"
  equipment_used:
  - equipment_name: Confocal Microscope
    manufacturer_model: Foster-Blackburn Image1738
    settings_parameters: "6918 x g, 32\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Hughes-Flores Authority4257
  procedure_steps:
  - step_description: Cells were visualized with pbs to facilitate bad.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 17
    temperature_celsius: 10
    replicates: 2
  - step_description: Cells were transfected with anti-ha antibody to facilitate continue.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    duration_minutes: 301
control_groups:
- control_type: Vehicle Control
  description: Research really require million contain add hospital.
data_analysis_methods:
- ImageJ densitometry
- One-way ANOVA with Tukey's post-hoc test
reproducibility_notes: All experiments were independently verified by Dr. Johnny Williams
  and results were consistent across multiple biological replicates.
