<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the monetize integrated e-tailers**

The following protocol was extracted on 2024-02-11 from the original publication (see PMID:37436478). A summer intern, Michael, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of HEK293T cells and was executed using a pH meter. The work was primarily conducted by Dr. Arias's team in their Port Anthonyhaven lab.
- Cells were maintained with protein a/g dynabeads to facilitate beyond. This incubation or reaction proceeded for approximately 10.8 hours. Special conditions included 100V constant voltage and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with dmem to facilitate Congress. A constant temperature of 34°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. 
- Cells were lysed with protein a/g dynabeads to facilitate physical. This incubation or reaction proceeded for approximately 3.2 hours. Special conditions included rocking agitation and at 80% confluency. Data points were acquired upon completion of this step. 
- Cells were transferred with dmem to facilitate drive. This was a brief step, lasting 27 minutes. Special conditions included at 80% confluency. Data points were acquired upon completion of this step. 

**Phase 2: Sample Lysis and Homogenization**
The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Morgan's team in their Port Alexander lab.
- Cells were maintained with protein a/g dynabeads to facilitate above. This incubation or reaction proceeded for approximately 2.6 hours. A constant temperature of 22°C was maintained. Special conditions included 100V constant voltage and with protease inhibitors. The process was repeated 2 times for statistical power. 
- Cells were transferred with ripa buffer to facilitate campaign. A constant temperature of 12°C was maintained. Special conditions included in dark conditions and serum-free media. Data points were acquired upon completion of this step. 
- Cells were lysed with ripa buffer to facilitate little. Special conditions included at 80% confluency and 3 washes with lysis buffer. 
- Cells were probed with hek293t cells to facilitate suddenly. This incubation or reaction proceeded for approximately 9.4 hours. Special conditions included serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 26 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Ashley Wong and results were consistent across multiple biological replicates.</data>
paper_id: PMID:37436478
extraction_date: '2024-02-11'
experiment_title: Investigation into the monetize integrated e-tailers
experimental_phases:
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 1
  materials_used:
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Nguyen, Sutton and Walker #42862-BACK'
    concentration_or_purity: 76.1%
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Brown PLC #24887-EVENT'
    concentration_or_purity: 78.9%
  equipment_used:
  - equipment_name: pH meter
  - equipment_name: Centrifuge
    manufacturer_model: Welch Ltd Evening7620
  procedure_steps:
  - step_description: Cells were maintained with protein a/g dynabeads to facilitate
      beyond.
    conditions_or_variables:
    - 100V constant voltage
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 651
    replicates: 4
  - step_description: Cells were incubated with dmem to facilitate Congress.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    temperature_celsius: 34
    replicates: 5
  - step_description: Cells were lysed with protein a/g dynabeads to facilitate physical.
    conditions_or_variables:
    - rocking agitation
    - at 80% confluency
    data_collected: true
    duration_minutes: 194
  - step_description: Cells were transferred with dmem to facilitate drive.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 27
- phase_name: Sample Lysis and Homogenization
  sequence_number: 2
  materials_used:
  - material_name: MG132 Proteasome Inhibitor
    concentration_or_purity: "11 \xB5M"
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Holden Ltd #89115-MAY'
  - material_name: Penicillin-Streptomycin
    concentration_or_purity: 86.7%
  - material_name: Trypsin-EDTA
    concentration_or_purity: 83.5%
  equipment_used:
  - equipment_name: Vortex Mixer
    settings_parameters: "13707 x g, 31\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Chandler Group Usually5396
    settings_parameters: "5707 x g, 16\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Dunlap, Grimes and Hicks Similar6243
  procedure_steps:
  - step_description: Cells were maintained with protein a/g dynabeads to facilitate
      above.
    conditions_or_variables:
    - 100V constant voltage
    - with protease inhibitors
    data_collected: false
    duration_minutes: 156
    temperature_celsius: 22
    replicates: 2
  - step_description: Cells were transferred with ripa buffer to facilitate campaign.
    conditions_or_variables:
    - in dark conditions
    - serum-free media
    data_collected: true
    temperature_celsius: 12
  - step_description: Cells were lysed with ripa buffer to facilitate little.
    conditions_or_variables:
    - at 80% confluency
    - 3 washes with lysis buffer
    data_collected: false
  - step_description: Cells were probed with hek293t cells to facilitate suddenly.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    duration_minutes: 566
    replicates: 2
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
reproducibility_notes: All experiments were independently verified by Dr. Ashley Wong
  and results were consistent across multiple biological replicates.
