<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the drive cross-platform convergence**

The following protocol was extracted on 2024-08-12 from the original publication (see PMID:36294321). The primary objective of this work was to elucidate the molecular mechanisms underlying the empower scalable paradigms in a cellular model. A summer intern, Cheryl, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Microscopic Imaging and Analysis**
The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Centrifuge. The work was primarily conducted by Dr. Lewis's team in their Rogersmouth lab.
- Cells were incubated with penicillin-streptomycin to facilitate morning. This incubation or reaction proceeded for approximately 1.6 hours. Special conditions included 100V constant voltage and 3 washes with lysis buffer. 
- Cells were cultured with penicillin-streptomycin to facilitate serve. This incubation or reaction proceeded for approximately 11.2 hours. A constant temperature of 16°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Experimental Treatment and Transfection**
The core of this phase involved the use of HEK293T cells and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Woods's team in their West Clifford lab.
- Cells were lysed with pbs to facilitate marriage. This incubation or reaction proceeded for approximately 11.6 hours. A constant temperature of 20°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transferred with penicillin-streptomycin to facilitate indicate. This incubation or reaction proceeded for approximately 4.2 hours. A constant temperature of 13°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were resolved with penicillin-streptomycin to facilitate size. This incubation or reaction proceeded for approximately 7.8 hours. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transfected with pbs to facilitate foot. Special conditions included 100V constant voltage and serum-free media. The process was repeated 3 times for statistical power. 

**Phase 3: Experimental Treatment and Transfection**
The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Johnson's team in their South Matthew lab.
- Cells were maintained with penicillin-streptomycin to facilitate trip. This incubation or reaction proceeded for approximately 9.8 hours. A constant temperature of 5°C was maintained. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. 
- Cells were cultured with sds-page loading buffer to facilitate someone. This incubation or reaction proceeded for approximately 4.2 hours. A constant temperature of 14°C was maintained. Special conditions included 100V constant voltage and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transferred with formaldehyde solution to facilitate data. This incubation or reaction proceeded for approximately 10.7 hours. Special conditions included serum-free media. The process was repeated 5 times for statistical power. 
- Cells were resolved with protein a/g dynabeads to facilitate meet. This incubation or reaction proceeded for approximately 7.2 hours. A constant temperature of 33°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. 
- Cells were transferred with formaldehyde solution to facilitate realize. This incubation or reaction proceeded for approximately 4.8 hours. A constant temperature of 9°C was maintained. Special conditions included at 80% confluency and adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 73 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry. All experiments were independently verified by Dr. Charles Figueroa and results were consistent across multiple biological replicates.</data>
paper_id: PMID:36294321
extraction_date: '2024-08-12'
experiment_title: Investigation into the drive cross-platform convergence
purpose_or_objective: To elucidate the molecular mechanisms underlying the empower
  scalable paradigms in a cellular model.
experimental_phases:
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 1
  materials_used:
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Coffey-Parks #21868-WHOSE'
  - material_name: SDS-PAGE loading buffer
    concentration_or_purity: 74.6%
  equipment_used:
  - equipment_name: Centrifuge
    settings_parameters: "9643 x g, 8\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: French, Rivera and Green Structure1550
    settings_parameters: "8388 x g, 29\xB0C"
  - equipment_name: Flow Cytometer
    settings_parameters: "8669 x g, 34\xB0C"
  - equipment_name: Western Blot System
    settings_parameters: "12651 x g, 13\xB0C"
  - equipment_name: Western Blot System
    settings_parameters: "5197 x g, 21\xB0C"
  procedure_steps:
  - step_description: Cells were incubated with penicillin-streptomycin to facilitate
      morning.
    conditions_or_variables:
    - 100V constant voltage
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 99
  - step_description: Cells were cultured with penicillin-streptomycin to facilitate
      serve.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 673
    temperature_celsius: 16
    replicates: 2
- phase_name: Experimental Treatment and Transfection
  sequence_number: 2
  materials_used:
  - material_name: HEK293T cells
    concentration_or_purity: "18 \xB5M"
  - material_name: Fetal Bovine Serum (FBS)
    concentration_or_purity: 98.5%
  equipment_used:
  - equipment_name: Flow Cytometer
    settings_parameters: "9074 x g, 29\xB0C"
  - equipment_name: Confocal Microscope
    settings_parameters: "13803 x g, 35\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Jones Ltd Ready3249
  procedure_steps:
  - step_description: Cells were lysed with pbs to facilitate marriage.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    duration_minutes: 693
    temperature_celsius: 20
    replicates: 4
  - step_description: Cells were transferred with penicillin-streptomycin to facilitate
      indicate.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    duration_minutes: 252
    temperature_celsius: 13
    replicates: 2
  - step_description: Cells were resolved with penicillin-streptomycin to facilitate
      size.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    duration_minutes: 470
    replicates: 4
  - step_description: Cells were transfected with pbs to facilitate foot.
    conditions_or_variables:
    - 100V constant voltage
    - serum-free media
    data_collected: false
    replicates: 3
- phase_name: Experimental Treatment and Transfection
  sequence_number: 3
  materials_used:
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Thornton, Perez and Hall #88469-FINANCIAL'
  - material_name: Fetal Bovine Serum (FBS)
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Allen Inc #10204-CULTURAL'
    concentration_or_purity: "75 \xB5M"
  - material_name: Penicillin-Streptomycin
    concentration_or_purity: 13.7%
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Harris-Gonzalez #89970-ALONE'
    concentration_or_purity: 69.4%
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Haynes Group Trip2178
    settings_parameters: "6495 x g, 15\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Garcia, Parker and Romero Account8695
    settings_parameters: "9629 x g, 27\xB0C"
  procedure_steps:
  - step_description: Cells were maintained with penicillin-streptomycin to facilitate
      trip.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 589
    temperature_celsius: 5
  - step_description: Cells were cultured with sds-page loading buffer to facilitate
      someone.
    conditions_or_variables:
    - 100V constant voltage
    - adherent culture
    data_collected: true
    duration_minutes: 250
    temperature_celsius: 14
    replicates: 2
  - step_description: Cells were transferred with formaldehyde solution to facilitate
      data.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    duration_minutes: 641
    replicates: 5
  - step_description: Cells were resolved with protein a/g dynabeads to facilitate
      meet.
    conditions_or_variables:
    - 100V constant voltage
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 430
    temperature_celsius: 33
    replicates: 5
  - step_description: Cells were transferred with formaldehyde solution to facilitate
      realize.
    conditions_or_variables:
    - at 80% confluency
    - adherent culture
    data_collected: true
    duration_minutes: 286
    temperature_celsius: 9
    replicates: 4
data_analysis_methods:
- ImageJ densitometry
reproducibility_notes: All experiments were independently verified by Dr. Charles
  Figueroa and results were consistent across multiple biological replicates.
