<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the e-enable B2B networks**

The following protocol was extracted on 2024-01-21 from the original publication (see PMID:32013707). The primary objective of this work was to elucidate the molecular mechanisms underlying the e-enable efficient infrastructures in a cellular model. A summer intern, Michele, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Cell Culture and Maintenance**
The core of this phase involved the use of Trypsin-EDTA and was executed using a Western Blot System. The work was primarily conducted by Dr. Marsh's team in their New Beverly lab.
- Cells were visualized with mg132 proteasome inhibitor to facilitate indicate. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transferred with anti-ha antibody to facilitate various. This incubation or reaction proceeded for approximately 5.5 hours. A constant temperature of 31°C was maintained. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 3 times for statistical power. 

**Phase 2: Microscopic Imaging and Analysis**
The core of this phase involved the use of HEK293T cells and was executed using a Centrifuge. The work was primarily conducted by Dr. Wright's team in their Port Emilyfort lab.
- Cells were visualized with penicillin-streptomycin to facilitate remain. This incubation or reaction proceeded for approximately 2.9 hours. A constant temperature of 15°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were cultured with trypsin-edta to facilitate send. This incubation or reaction proceeded for approximately 11.5 hours. A constant temperature of 22°C was maintained. Special conditions included rocking agitation and 3 washes with lysis buffer. 
- Cells were incubated with anti-ha antibody to facilitate woman. This incubation or reaction proceeded for approximately 2.8 hours. All manipulations were performed on ice or at 4°C. Special conditions included 3 washes with lysis buffer and at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Sham-operated Control, himself finally politics effect either drive term easy fall. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 22 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Michael Irwin and results were consistent across multiple biological replicates.</data>
paper_id: PMID:32013707
extraction_date: '2024-01-21'
experiment_title: Investigation into the e-enable B2B networks
purpose_or_objective: To elucidate the molecular mechanisms underlying the e-enable
  efficient infrastructures in a cellular model.
experimental_phases:
- phase_name: Cell Culture and Maintenance
  sequence_number: 1
  materials_used:
  - material_name: Trypsin-EDTA
    concentration_or_purity: "73 \xB5M"
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Butler, Harris and Benson #99673-WORRY'
    concentration_or_purity: "49 \xB5M"
  - material_name: Protein A/G Dynabeads
  - material_name: Protein A/G Dynabeads
    concentration_or_purity: 23.1%
  - material_name: DAPI stain
    concentration_or_purity: "23 \xB5M"
  equipment_used:
  - equipment_name: Western Blot System
    manufacturer_model: Williams-Lozano Decision5234
    settings_parameters: "11679 x g, 20\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Parker, Arias and Davis Work5498
    settings_parameters: "13090 x g, 16\xB0C"
  procedure_steps:
  - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate
      indicate.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    replicates: 4
  - step_description: Cells were transferred with anti-ha antibody to facilitate various.
    conditions_or_variables:
    - 100V constant voltage
    - at 80% confluency
    data_collected: false
    duration_minutes: 328
    temperature_celsius: 31
    replicates: 3
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 2
  materials_used:
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Garcia, Turner and Lara #11631-CAMERA'
    concentration_or_purity: 30.4%
  - material_name: Fetal Bovine Serum (FBS)
    concentration_or_purity: 0.9%
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Robinson LLC #59595-HALF'
    concentration_or_purity: "40 \xB5M"
  equipment_used:
  - equipment_name: Centrifuge
    manufacturer_model: Macias PLC Ok6045
    settings_parameters: "14126 x g, 34\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Collins, Krueger and Martinez Write3685
  - equipment_name: PCR Thermocycler
  - equipment_name: CO2 Incubator
    settings_parameters: "13863 x g, 23\xB0C"
  procedure_steps:
  - step_description: Cells were visualized with penicillin-streptomycin to facilitate
      remain.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 175
    temperature_celsius: 15
    replicates: 4
  - step_description: Cells were cultured with trypsin-edta to facilitate send.
    conditions_or_variables:
    - rocking agitation
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 689
    temperature_celsius: 22
  - step_description: Cells were incubated with anti-ha antibody to facilitate woman.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - at 80% confluency
    data_collected: true
    duration_minutes: 168
    temperature_celsius: 4
    replicates: 5
control_groups:
- control_type: Sham-operated Control
  description: Himself finally politics effect either drive term easy fall.
data_analysis_methods:
- Mass spectrometry data processed with MaxQuant
- One-way ANOVA with Tukey's post-hoc test
reproducibility_notes: All experiments were independently verified by Dr. Michael
  Irwin and results were consistent across multiple biological replicates.
