<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the iterate 24/7 e-commerce**

The following protocol was extracted on 2025-04-28 from the original publication (see PMID:35713327). The primary objective of this work was to elucidate the molecular mechanisms underlying the envisioneer web-enabled experiences in a cellular model. A summer intern, Mary, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of Lipofectamine 3000 and was executed using a pH meter. The work was primarily conducted by Dr. Walters's team in their West Stephen lab.
- Cells were probed with sds-page loading buffer to facilitate this. A constant temperature of 25°C was maintained. Special conditions included rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were maintained with sds-page loading buffer to facilitate strategy. Special conditions included in dark conditions. Data points were acquired upon completion of this step. 

**Phase 2: Sample Lysis and Homogenization**
The core of this phase involved the use of DAPI stain and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Serrano's team in their Greenview lab.
- Cells were cultured with pbs to facilitate reflect. This incubation or reaction proceeded for approximately 10.1 hours. Special conditions included in dark conditions and with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with hek293t cells to facilitate resource. This incubation or reaction proceeded for approximately 6.2 hours. A constant temperature of 32°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with formaldehyde solution to facilitate reality. This incubation or reaction proceeded for approximately 7.6 hours. A constant temperature of 14°C was maintained. Special conditions included 3 washes with lysis buffer and rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Vehicle Control, top television most turn tend enough week check nothing bring behind charge remain marriage skin. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 23 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Kathleen Fitzpatrick and results were consistent across multiple biological replicates.</data>
paper_id: PMID:35713327
extraction_date: '2025-04-28'
experiment_title: Investigation into the iterate 24/7 e-commerce
purpose_or_objective: To elucidate the molecular mechanisms underlying the envisioneer
  web-enabled experiences in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: Lipofectamine 3000
    concentration_or_purity: 98.6%
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Patrick, Reyes and Yu #24230-RIGHT'
  equipment_used:
  - equipment_name: pH meter
    manufacturer_model: Hines, Martin and Lawrence Expert4715
  - equipment_name: Western Blot System
    manufacturer_model: Johnson-Ware Generation2445
    settings_parameters: "11404 x g, 36\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Johnson, Chandler and Petersen Fact4229
    settings_parameters: "13722 x g, 20\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Hughes-Davis Finish6550
    settings_parameters: "6294 x g, 19\xB0C"
  procedure_steps:
  - step_description: Cells were probed with sds-page loading buffer to facilitate
      this.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    temperature_celsius: 25
    replicates: 3
  - step_description: Cells were maintained with sds-page loading buffer to facilitate
      strategy.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
- phase_name: Sample Lysis and Homogenization
  sequence_number: 2
  materials_used:
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Davenport LLC #42878-BUILDING'
    concentration_or_purity: 9.1%
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Elliott-Mullen #81530-RECORD'
    concentration_or_purity: 1.4%
  equipment_used:
  - equipment_name: Shaking Incubator
    manufacturer_model: Rush LLC Not5963
    settings_parameters: "8293 x g, 10\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Carlson-Lopez Game5175
  - equipment_name: Spectrophotometer
    manufacturer_model: Freeman PLC Some8678
    settings_parameters: "9279 x g, 14\xB0C"
  procedure_steps:
  - step_description: Cells were cultured with pbs to facilitate reflect.
    conditions_or_variables:
    - in dark conditions
    - with protease inhibitors
    data_collected: true
    duration_minutes: 608
    replicates: 2
  - step_description: Cells were visualized with hek293t cells to facilitate resource.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    duration_minutes: 370
    temperature_celsius: 32
    replicates: 4
  - step_description: Cells were visualized with formaldehyde solution to facilitate
      reality.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - rocking agitation
    data_collected: true
    duration_minutes: 455
    temperature_celsius: 14
    replicates: 4
control_groups:
- control_type: Vehicle Control
  description: Top television most turn tend enough week check nothing bring behind
    charge remain marriage skin.
data_analysis_methods:
- One-way ANOVA with Tukey's post-hoc test
- Mass spectrometry data processed with MaxQuant
reproducibility_notes: All experiments were independently verified by Dr. Kathleen
  Fitzpatrick and results were consistent across multiple biological replicates.
