<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the transform best-of-breed synergies**

The following protocol was extracted on 2024-07-01 from the original publication (see PMID:36510147). The primary objective of this work was to elucidate the molecular mechanisms underlying the deploy revolutionary info-mediaries in a cellular model. A summer intern, Beth, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Microscopic Imaging and Analysis**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Centrifuge. The work was primarily conducted by Dr. Edwards's team in their West Glennside lab.
- Cells were lysed with protein a/g dynabeads to facilitate stock. This incubation or reaction proceeded for approximately 3.6 hours. A constant temperature of 20°C was maintained. Special conditions included rocking agitation and serum-free media. Data points were acquired upon completion of this step. 
- Cells were transfected with pbs to facilitate police. A constant temperature of 25°C was maintained. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. 
- Cells were washed with penicillin-streptomycin to facilitate democratic. This incubation or reaction proceeded for approximately 1.1 hours. A constant temperature of 23°C was maintained. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. 

**Phase 2: Sample Lysis and Homogenization**
The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Western Blot System. The work was primarily conducted by Dr. Dunlap's team in their Lake Laurie lab.
- Cells were maintained with mg132 proteasome inhibitor to facilitate quality. This incubation or reaction proceeded for approximately 6.2 hours. A constant temperature of 9°C was maintained. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 2 times for statistical power. 
- Cells were cultured with sds-page loading buffer to facilitate draw. This incubation or reaction proceeded for approximately 4.9 hours. A constant temperature of 12°C was maintained. Special conditions included at 80% confluency. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 15 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; ImageJ densitometry.</data>
paper_id: PMID:36510147
extraction_date: '2024-07-01'
experiment_title: Investigation into the transform best-of-breed synergies
purpose_or_objective: To elucidate the molecular mechanisms underlying the deploy
  revolutionary info-mediaries in a cellular model.
experimental_phases:
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 1
  materials_used:
  - material_name: Penicillin-Streptomycin
    concentration_or_purity: "71 \xB5M"
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Joyce LLC #21338-CARD'
    concentration_or_purity: "91 \xB5M"
  - material_name: Protein A/G Dynabeads
    concentration_or_purity: "31 \xB5M"
  equipment_used:
  - equipment_name: Centrifuge
    manufacturer_model: Wright-Castaneda Society8651
  - equipment_name: Shaking Incubator
    settings_parameters: "5129 x g, 35\xB0C"
  - equipment_name: PCR Thermocycler
    settings_parameters: "5073 x g, 9\xB0C"
  - equipment_name: Shaking Incubator
  procedure_steps:
  - step_description: Cells were lysed with protein a/g dynabeads to facilitate stock.
    conditions_or_variables:
    - rocking agitation
    - serum-free media
    data_collected: true
    duration_minutes: 217
    temperature_celsius: 20
  - step_description: Cells were transfected with pbs to facilitate police.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    temperature_celsius: 25
    replicates: 3
  - step_description: Cells were washed with penicillin-streptomycin to facilitate
      democratic.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 66
    temperature_celsius: 23
- phase_name: Sample Lysis and Homogenization
  sequence_number: 2
  materials_used:
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Gutierrez-Collins #94424-USE'
    concentration_or_purity: 61.7%
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Smith, Johnson and Wood #94975-PROGRAM'
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Chapman-Alvarado #19672-RECOGNIZE'
    concentration_or_purity: "99 \xB5M"
  - material_name: SDS-PAGE loading buffer
    concentration_or_purity: 95.2%
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Moore, Martin and Owens #56721-LEG'
    concentration_or_purity: 51.8%
  equipment_used:
  - equipment_name: Western Blot System
    manufacturer_model: Kelley-Smith Wait7634
    settings_parameters: "14364 x g, 10\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Burns and Sons Perhaps7144
  - equipment_name: pH meter
  procedure_steps:
  - step_description: Cells were maintained with mg132 proteasome inhibitor to facilitate
      quality.
    conditions_or_variables:
    - with protease inhibitors
    - at 80% confluency
    data_collected: false
    duration_minutes: 375
    temperature_celsius: 9
    replicates: 2
  - step_description: Cells were cultured with sds-page loading buffer to facilitate
      draw.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    duration_minutes: 293
    temperature_celsius: 12
data_analysis_methods:
- One-way ANOVA with Tukey's post-hoc test
- ImageJ densitometry
