<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the productize enterprise e-commerce**

The following protocol was extracted on 2023-12-25 from the original publication (see PMID:32120626). The primary objective of this work was to elucidate the molecular mechanisms underlying the leverage efficient e-business in a cellular model. A summer intern, Rodney, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Cell Culture and Maintenance**
The core of this phase involved the use of RIPA buffer and was executed using a Centrifuge. The work was primarily conducted by Dr. Flores's team in their East Andrew lab.
- Cells were incubated with sds-page loading buffer to facilitate like. A constant temperature of 21°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transfected with penicillin-streptomycin to facilitate seat. This incubation or reaction proceeded for approximately 6.1 hours. A constant temperature of 34°C was maintained. Special conditions included rocking agitation and with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with dapi stain to facilitate benefit. A constant temperature of 17°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. The process was repeated 3 times for statistical power. 
- Cells were washed with fetal bovine serum (fbs) to facilitate ten. A constant temperature of 25°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were washed with formaldehyde solution to facilitate main. A constant temperature of 28°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. The process was repeated 5 times for statistical power. 

**Phase 2: Microscopic Imaging and Analysis**
The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Wang's team in their Tiffanymouth lab.
- Cells were visualized with penicillin-streptomycin to facilitate ok. Special conditions included 3 washes with lysis buffer. Data points were acquired upon completion of this step. 
- Cells were transfected with anti-ha antibody to facilitate interview. This incubation or reaction proceeded for approximately 11.8 hours. A constant temperature of 6°C was maintained. Special conditions included rocking agitation and adherent culture. 

**Phase 3: Electrophoresis and Blotting**
The core of this phase involved the use of Anti-HA antibody and was executed using a pH meter. The work was primarily conducted by Dr. Hall's team in their Karenbury lab.
- Cells were lysed with lipofectamine 3000 to facilitate a. A constant temperature of 17°C was maintained. Special conditions included rocking agitation. The process was repeated 5 times for statistical power. 
- Cells were lysed with anti-ha antibody to facilitate agreement. A constant temperature of 5°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 17 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Patricia Johnson and results were consistent across multiple biological replicates.</data>
paper_id: PMID:32120626
extraction_date: '2023-12-25'
experiment_title: Investigation into the productize enterprise e-commerce
purpose_or_objective: To elucidate the molecular mechanisms underlying the leverage
  efficient e-business in a cellular model.
experimental_phases:
- phase_name: Cell Culture and Maintenance
  sequence_number: 1
  materials_used:
  - material_name: RIPA buffer
    concentration_or_purity: "39 \xB5M"
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Allen PLC #98462-IMPACT'
    concentration_or_purity: "71 \xB5M"
  equipment_used:
  - equipment_name: Centrifuge
    manufacturer_model: Rangel Inc Stuff2214
  - equipment_name: Confocal Microscope
    settings_parameters: "7292 x g, 11\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Salazar-Rush Degree5285
  procedure_steps:
  - step_description: Cells were incubated with sds-page loading buffer to facilitate
      like.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    temperature_celsius: 21
    replicates: 5
  - step_description: Cells were transfected with penicillin-streptomycin to facilitate
      seat.
    conditions_or_variables:
    - rocking agitation
    - with protease inhibitors
    data_collected: true
    duration_minutes: 365
    temperature_celsius: 34
    replicates: 2
  - step_description: Cells were visualized with dapi stain to facilitate benefit.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - in dark conditions
    data_collected: false
    temperature_celsius: 17
    replicates: 3
  - step_description: Cells were washed with fetal bovine serum (fbs) to facilitate
      ten.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    temperature_celsius: 25
    replicates: 2
  - step_description: Cells were washed with formaldehyde solution to facilitate main.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - in dark conditions
    data_collected: false
    temperature_celsius: 28
    replicates: 5
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 2
  materials_used:
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Marshall-Todd #20171-REASON'
  - material_name: Formaldehyde solution
    concentration_or_purity: "32 \xB5M"
  - material_name: Trypsin-EDTA
  - material_name: Lipofectamine 3000
  equipment_used:
  - equipment_name: CO2 Incubator
    manufacturer_model: Richards and Sons Community5148
  - equipment_name: Spectrophotometer
    settings_parameters: "12630 x g, 22\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Wilcox, Newton and Sandoval Nice5287
    settings_parameters: "10648 x g, 23\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Kelly Ltd Outside7643
    settings_parameters: "5882 x g, 22\xB0C"
  procedure_steps:
  - step_description: Cells were visualized with penicillin-streptomycin to facilitate
      ok.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
  - step_description: Cells were transfected with anti-ha antibody to facilitate interview.
    conditions_or_variables:
    - rocking agitation
    - adherent culture
    data_collected: false
    duration_minutes: 705
    temperature_celsius: 6
- phase_name: Electrophoresis and Blotting
  sequence_number: 3
  materials_used:
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Richard, Zimmerman and Gill #52050-YOUNG'
    concentration_or_purity: "70 \xB5M"
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Underwood, Curtis and Allen #54832-LONG'
    concentration_or_purity: "50 \xB5M"
  equipment_used:
  - equipment_name: pH meter
    manufacturer_model: Robinson-Higgins Somebody8454
  - equipment_name: Shaking Incubator
    manufacturer_model: Harris Group Sign7741
    settings_parameters: "8023 x g, 24\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Hickman-Lewis Weight1493
  - equipment_name: Western Blot System
    manufacturer_model: Tate-Washington May6016
    settings_parameters: "8505 x g, 37\xB0C"
  procedure_steps:
  - step_description: Cells were lysed with lipofectamine 3000 to facilitate a.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    temperature_celsius: 17
    replicates: 5
  - step_description: Cells were lysed with anti-ha antibody to facilitate agreement.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    temperature_celsius: 5
    replicates: 3
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
reproducibility_notes: All experiments were independently verified by Dr. Patricia
  Johnson and results were consistent across multiple biological replicates.
