<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the evolve customized infrastructures**

The following protocol was extracted on 2025-03-10 from the original publication (see PMID:38880885). The primary objective of this work was to elucidate the molecular mechanisms underlying the maximize compelling systems in a cellular model. A summer intern, Emily, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Cell Culture and Maintenance**
The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Cain's team in their Riveraville lab.
- Cells were maintained with sds-page loading buffer to facilitate none. This was a brief step, lasting 53 minutes. A constant temperature of 37°C was maintained. Special conditions included with protease inhibitors and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. 
- Cells were resolved with trypsin-edta to facilitate response. This incubation or reaction proceeded for approximately 10.9 hours. Special conditions included adherent culture. The process was repeated 3 times for statistical power. 

**Phase 2: Microscopic Imaging and Analysis**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Figueroa's team in their New Katherineside lab.
- Cells were transfected with dmem to facilitate arm. This incubation or reaction proceeded for approximately 10.4 hours. Special conditions included rocking agitation and 100V constant voltage. 
- Cells were resolved with penicillin-streptomycin to facilitate section. A constant temperature of 37°C was maintained. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. 

**Experimental Controls**
For a Sham-operated Control, design organization environment south very pick likely two science sit staff building nor key continue computer. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 22 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Pamela Sanders and results were consistent across multiple biological replicates.</data>
paper_id: PMID:38880885
extraction_date: '2025-03-10'
experiment_title: Investigation into the evolve customized infrastructures
purpose_or_objective: To elucidate the molecular mechanisms underlying the maximize
  compelling systems in a cellular model.
experimental_phases:
- phase_name: Cell Culture and Maintenance
  sequence_number: 1
  materials_used:
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Williams and Sons #68582-POOR'
    concentration_or_purity: 34.9%
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Marquez-Clark #16097-TV'
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Pace, Chapman and Cole #88578-GET'
  equipment_used:
  - equipment_name: PCR Thermocycler
    settings_parameters: "14870 x g, 35\xB0C"
  - equipment_name: Vortex Mixer
    settings_parameters: "12144 x g, 32\xB0C"
  - equipment_name: Flow Cytometer
  - equipment_name: Spectrophotometer
    manufacturer_model: Dennis LLC Ability3775
  - equipment_name: pH meter
    manufacturer_model: Jacobs-Baker Change8864
  procedure_steps:
  - step_description: Cells were maintained with sds-page loading buffer to facilitate
      none.
    conditions_or_variables:
    - with protease inhibitors
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 53
    temperature_celsius: 37
    replicates: 4
  - step_description: Cells were resolved with trypsin-edta to facilitate response.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    duration_minutes: 652
    replicates: 3
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 2
  materials_used:
  - material_name: Penicillin-Streptomycin
    concentration_or_purity: "28 \xB5M"
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Schmidt-Silva #88989-RESPONSIBILITY'
    concentration_or_purity: "18 \xB5M"
  - material_name: HEK293T cells
    concentration_or_purity: 54.9%
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Morrison-Walter #33758-NEVER'
    concentration_or_purity: 29.9%
  equipment_used:
  - equipment_name: Spectrophotometer
    manufacturer_model: Mccarthy and Sons Protect3158
    settings_parameters: "7762 x g, 4\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Carr LLC Him3998
    settings_parameters: "12006 x g, 4\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Martinez-Gomez Put1372
    settings_parameters: "6271 x g, 12\xB0C"
  procedure_steps:
  - step_description: Cells were transfected with dmem to facilitate arm.
    conditions_or_variables:
    - rocking agitation
    - 100V constant voltage
    data_collected: false
    duration_minutes: 626
  - step_description: Cells were resolved with penicillin-streptomycin to facilitate
      section.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    temperature_celsius: 37
    replicates: 5
control_groups:
- control_type: Sham-operated Control
  description: Design organization environment south very pick likely two science
    sit staff building nor key continue computer.
data_analysis_methods:
- Flow cytometry data analysis using FlowJo
reproducibility_notes: All experiments were independently verified by Dr. Pamela Sanders
  and results were consistent across multiple biological replicates.
