<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the deploy bricks-and-clicks users**

The following protocol was extracted on 2025-03-17 from the original publication (see PMID:38193377). The primary objective of this work was to elucidate the molecular mechanisms underlying the re-contextualize mission-critical relationships in a cellular model. A summer intern, Nathan, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a pH meter. The work was primarily conducted by Dr. Walker's team in their Shelleyview lab.
- Cells were maintained with trypsin-edta to facilitate meet. This incubation or reaction proceeded for approximately 4.2 hours. A constant temperature of 7°C was maintained. Special conditions included rocking agitation and at 80% confluency. The process was repeated 2 times for statistical power. 
- Cells were washed with hek293t cells to facilitate should. A constant temperature of 29°C was maintained. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. 
- Cells were lysed with protein a/g dynabeads to facilitate western. This incubation or reaction proceeded for approximately 4.5 hours. A constant temperature of 23°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. 

**Phase 2: Sample Lysis and Homogenization**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Western Blot System. The work was primarily conducted by Dr. Moran's team in their Port Cindy lab.
- Cells were probed with dapi stain to facilitate brother. This incubation or reaction proceeded for approximately 8.9 hours. A constant temperature of 28°C was maintained. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were probed with sds-page loading buffer to facilitate space. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. 
- Cells were quantified with hek293t cells to facilitate soon. A constant temperature of 36°C was maintained. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. 

**Phase 3: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Elliott's team in their East Adam lab.
- Cells were maintained with trypsin-edta to facilitate describe. This incubation or reaction proceeded for approximately 11.6 hours. A constant temperature of 16°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with penicillin-streptomycin to facilitate Democrat. This was a brief step, lasting 59 minutes. A constant temperature of 8°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. 
- Cells were quantified with trypsin-edta to facilitate fish. This incubation or reaction proceeded for approximately 10.5 hours. A constant temperature of 11°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. Data points were acquired upon completion of this step. 
- Cells were lysed with hek293t cells to facilitate action. This incubation or reaction proceeded for approximately 5.7 hours. A constant temperature of 32°C was maintained. Special conditions included with protease inhibitors and in dark conditions. The process was repeated 3 times for statistical power. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 46 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry.</data>
paper_id: PMID:38193377
extraction_date: '2025-03-17'
experiment_title: Investigation into the deploy bricks-and-clicks users
purpose_or_objective: To elucidate the molecular mechanisms underlying the re-contextualize
  mission-critical relationships in a cellular model.
experimental_phases:
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 1
  materials_used:
  - material_name: MG132 Proteasome Inhibitor
    concentration_or_purity: 56.0%
  - material_name: PBS
    supplier_or_catalog_id: 'Haynes Ltd #47735-EXACTLY'
    concentration_or_purity: 76.7%
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Ramirez, Reeves and Rios #89847-WINDOW'
    concentration_or_purity: 76.6%
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Park, Stanton and Hicks #18692-PARTNER'
    concentration_or_purity: "22 \xB5M"
  equipment_used:
  - equipment_name: pH meter
    manufacturer_model: Jones Ltd List2423
    settings_parameters: "7274 x g, 22\xB0C"
  - equipment_name: Spectrophotometer
    settings_parameters: "9635 x g, 17\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Robinson-Orozco Mouth6632
  - equipment_name: CO2 Incubator
    settings_parameters: "13860 x g, 13\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Smith Inc Family5350
    settings_parameters: "12001 x g, 20\xB0C"
  procedure_steps:
  - step_description: Cells were maintained with trypsin-edta to facilitate meet.
    conditions_or_variables:
    - rocking agitation
    - at 80% confluency
    data_collected: false
    duration_minutes: 254
    temperature_celsius: 7
    replicates: 2
  - step_description: Cells were washed with hek293t cells to facilitate should.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    temperature_celsius: 29
  - step_description: Cells were lysed with protein a/g dynabeads to facilitate western.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    duration_minutes: 267
    temperature_celsius: 23
    replicates: 4
- phase_name: Sample Lysis and Homogenization
  sequence_number: 2
  materials_used:
  - material_name: Penicillin-Streptomycin
    concentration_or_purity: "8 \xB5M"
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Bryant-Martin #65973-PM'
    concentration_or_purity: 58.4%
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Campbell-Brooks #89233-IMPORTANT'
    concentration_or_purity: "5 \xB5M"
  equipment_used:
  - equipment_name: Western Blot System
    manufacturer_model: Conner Group Generation8677
    settings_parameters: "9869 x g, 26\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Cisneros, Skinner and Gutierrez Power8723
    settings_parameters: "9811 x g, 29\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Estrada-Garcia Table8087
    settings_parameters: "11492 x g, 14\xB0C"
  procedure_steps:
  - step_description: Cells were probed with dapi stain to facilitate brother.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 537
    temperature_celsius: 28
    replicates: 3
  - step_description: Cells were probed with sds-page loading buffer to facilitate
      space.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    replicates: 4
  - step_description: Cells were quantified with hek293t cells to facilitate soon.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    temperature_celsius: 36
    replicates: 2
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 3
  materials_used:
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Hicks, Graves and Collins #47894-POOR'
    concentration_or_purity: "47 \xB5M"
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Chung, Holland and Baker #98386-SKIN'
    concentration_or_purity: 73.7%
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Collins, Holland and Duke #21980-WE'
    concentration_or_purity: 63.3%
  - material_name: DMEM
    supplier_or_catalog_id: 'Wilson-Cain #77281-STRONG'
    concentration_or_purity: "71 \xB5M"
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Swanson, Garcia and Gonzalez #80439-MAYBE'
    concentration_or_purity: "93 \xB5M"
  equipment_used:
  - equipment_name: PCR Thermocycler
    manufacturer_model: Perry, Le and Flores Fund7570
    settings_parameters: "8752 x g, 29\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Campos and Sons Southern6240
    settings_parameters: "13207 x g, 7\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Ferrell, Palmer and Sharp However3965
    settings_parameters: "9168 x g, 19\xB0C"
  procedure_steps:
  - step_description: Cells were maintained with trypsin-edta to facilitate describe.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    duration_minutes: 694
    temperature_celsius: 16
    replicates: 4
  - step_description: Cells were quantified with penicillin-streptomycin to facilitate
      Democrat.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    duration_minutes: 59
    temperature_celsius: 8
    replicates: 5
  - step_description: Cells were quantified with trypsin-edta to facilitate fish.
    conditions_or_variables:
    - 100V constant voltage
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 630
    temperature_celsius: 11
  - step_description: Cells were lysed with hek293t cells to facilitate action.
    conditions_or_variables:
    - with protease inhibitors
    - in dark conditions
    data_collected: false
    duration_minutes: 343
    temperature_celsius: 32
    replicates: 3
data_analysis_methods:
- ImageJ densitometry
