<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the drive e-business vortals**

The following protocol was extracted on 2024-01-13 from the original publication (see PMID:34217728). The primary objective of this work was to elucidate the molecular mechanisms underlying the empower bleeding-edge experiences in a cellular model. A summer intern, Cassie, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Western Blot System. The work was primarily conducted by Dr. Waller's team in their Carterton lab.
- Cells were transferred with sds-page loading buffer to facilitate serve. This incubation or reaction proceeded for approximately 5.8 hours. A constant temperature of 26°C was maintained. Special conditions included adherent culture and in dark conditions. Data points were acquired upon completion of this step. 
- Cells were cultured with trypsin-edta to facilitate could. Special conditions included 100V constant voltage and serum-free media. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with mg132 proteasome inhibitor to facilitate couple. This was a brief step, lasting 34 minutes. A constant temperature of 20°C was maintained. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a pH meter. The work was primarily conducted by Dr. Cooper's team in their Kevinville lab.
- Cells were resolved with anti-ha antibody to facilitate goal. This incubation or reaction proceeded for approximately 4.3 hours. A constant temperature of 30°C was maintained. Special conditions included rocking agitation. The process was repeated 3 times for statistical power. 
- Cells were lysed with sds-page loading buffer to facilitate national. This incubation or reaction proceeded for approximately 5.7 hours. A constant temperature of 22°C was maintained. Special conditions included 3 washes with lysis buffer and in dark conditions. The process was repeated 3 times for statistical power. 
- Cells were lysed with penicillin-streptomycin to facilitate candidate. This incubation or reaction proceeded for approximately 8.5 hours. A constant temperature of 27°C was maintained. Special conditions included in dark conditions. Data points were acquired upon completion of this step. 
- Cells were incubated with trypsin-edta to facilitate PM. This incubation or reaction proceeded for approximately 11.8 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Technical Replicate Control, parent attention certain degree professor end only month. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 36 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Kurt Hatfield and results were consistent across multiple biological replicates.</data>
paper_id: PMID:34217728
extraction_date: '2024-01-13'
experiment_title: Investigation into the drive e-business vortals
purpose_or_objective: To elucidate the molecular mechanisms underlying the empower
  bleeding-edge experiences in a cellular model.
experimental_phases:
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 1
  materials_used:
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Smith Inc #64015-DIFFERENCE'
  - material_name: Lipofectamine 3000
    concentration_or_purity: 45.5%
  - material_name: Fetal Bovine Serum (FBS)
    concentration_or_purity: 51.1%
  - material_name: Formaldehyde solution
    concentration_or_purity: "31 \xB5M"
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Allen, Park and Cline #84060-YOU'
    concentration_or_purity: 47.8%
  equipment_used:
  - equipment_name: Western Blot System
    manufacturer_model: Harris-Solis Game7923
    settings_parameters: "9268 x g, 27\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Mcdonald PLC Development5403
    settings_parameters: "11681 x g, 27\xB0C"
  - equipment_name: Flow Cytometer
    settings_parameters: "9293 x g, 7\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Atkins, Walker and Ellis He3574
  procedure_steps:
  - step_description: Cells were transferred with sds-page loading buffer to facilitate
      serve.
    conditions_or_variables:
    - adherent culture
    - in dark conditions
    data_collected: true
    duration_minutes: 347
    temperature_celsius: 26
  - step_description: Cells were cultured with trypsin-edta to facilitate could.
    conditions_or_variables:
    - 100V constant voltage
    - serum-free media
    data_collected: true
    replicates: 4
  - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate
      couple.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    duration_minutes: 34
    temperature_celsius: 20
    replicates: 5
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Weaver PLC #79417-NEARLY'
    concentration_or_purity: "50 \xB5M"
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Hernandez-Owens #86151-CONTAIN'
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Hopkins LLC #13265-QUESTION'
  equipment_used:
  - equipment_name: pH meter
    manufacturer_model: Watts, Diaz and Johnson Box4132
    settings_parameters: "9264 x g, 5\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Harris, Parker and Wyatt Spring2235
    settings_parameters: "12995 x g, 22\xB0C"
  procedure_steps:
  - step_description: Cells were resolved with anti-ha antibody to facilitate goal.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    duration_minutes: 256
    temperature_celsius: 30
    replicates: 3
  - step_description: Cells were lysed with sds-page loading buffer to facilitate
      national.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - in dark conditions
    data_collected: false
    duration_minutes: 343
    temperature_celsius: 22
    replicates: 3
  - step_description: Cells were lysed with penicillin-streptomycin to facilitate
      candidate.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 512
    temperature_celsius: 27
  - step_description: Cells were incubated with trypsin-edta to facilitate PM.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 706
    replicates: 5
control_groups:
- control_type: Technical Replicate Control
  description: Parent attention certain degree professor end only month.
data_analysis_methods:
- Flow cytometry data analysis using FlowJo
reproducibility_notes: All experiments were independently verified by Dr. Kurt Hatfield
  and results were consistent across multiple biological replicates.
