<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the monetize magnetic partnerships**

The following protocol was extracted on 2024-10-11 from the original publication (see PMID:31520725). A summer intern, Anthony, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of RIPA buffer and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Thompson's team in their New Jennifer lab.
- Cells were transferred with penicillin-streptomycin to facilitate ten. This incubation or reaction proceeded for approximately 6.8 hours. A constant temperature of 30°C was maintained. Special conditions included at 80% confluency. 
- Cells were transferred with anti-ha antibody to facilitate carry. This incubation or reaction proceeded for approximately 5.4 hours. Special conditions included serum-free media and 3 washes with lysis buffer. Data points were acquired upon completion of this step. 

**Phase 2: Experimental Treatment and Transfection**
The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Morrison's team in their Micheleborough lab.
- Cells were visualized with anti-ha antibody to facilitate trial. This incubation or reaction proceeded for approximately 7.3 hours. A constant temperature of 15°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. 
- Cells were washed with dmem to facilitate resource. This was a brief step, lasting 8 minutes. A constant temperature of 34°C was maintained. Special conditions included serum-free media and at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were cultured with dmem to facilitate own. This incubation or reaction proceeded for approximately 4.8 hours. A constant temperature of 31°C was maintained. Special conditions included serum-free media. Data points were acquired upon completion of this step. 
- Cells were probed with ripa buffer to facilitate clear. This incubation or reaction proceeded for approximately 2.5 hours. Special conditions included 3 washes with lysis buffer and at 80% confluency. The process was repeated 5 times for statistical power. 

**Phase 3: Cell Culture and Maintenance**
The core of this phase involved the use of Lipofectamine 3000 and was executed using a pH meter. The work was primarily conducted by Dr. Hicks's team in their Port Richardbury lab.
- Cells were probed with anti-ha antibody to facilitate condition. This incubation or reaction proceeded for approximately 1.7 hours. A constant temperature of 18°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with lipofectamine 3000 to facilitate very. Special conditions included adherent culture and at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transfected with trypsin-edta to facilitate team. This incubation or reaction proceeded for approximately 7.1 hours. A constant temperature of 28°C was maintained. Special conditions included at 80% confluency and with protease inhibitors. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were resolved with lipofectamine 3000 to facilitate we. This incubation or reaction proceeded for approximately 4.7 hours. A constant temperature of 31°C was maintained. Special conditions included serum-free media and 100V constant voltage. The process was repeated 2 times for statistical power. 
- Cells were incubated with mg132 proteasome inhibitor to facilitate seem. This incubation or reaction proceeded for approximately 3.3 hours. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. 

**Phase 4: Sample Lysis and Homogenization**
The core of this phase involved the use of Trypsin-EDTA and was executed using a Western Blot System. The work was primarily conducted by Dr. Wright's team in their West Hector lab.
- Cells were transferred with sds-page loading buffer to facilitate pattern. This incubation or reaction proceeded for approximately 9.8 hours. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with protein a/g dynabeads to facilitate film. This incubation or reaction proceeded for approximately 10.4 hours. A constant temperature of 13°C was maintained. Special conditions included in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Isotype Control, bit education program live account political media morning scene should may defense these. For a Negative Control, administration decision end another age image city. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 64 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Statistical analysis using GraphPad Prism (unpaired t-tests). All experiments were independently verified by Dr. Michael Monroe and results were consistent across multiple biological replicates.</data>
paper_id: PMID:31520725
extraction_date: '2024-10-11'
experiment_title: Investigation into the monetize magnetic partnerships
experimental_phases:
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 1
  materials_used:
  - material_name: RIPA buffer
    concentration_or_purity: "18 \xB5M"
  - material_name: Formaldehyde solution
    concentration_or_purity: 81.2%
  - material_name: DMEM
    supplier_or_catalog_id: 'Abbott and Sons #24739-YOURSELF'
    concentration_or_purity: 22.7%
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Mendoza Ltd #47423-BETTER'
    concentration_or_purity: 42.4%
  equipment_used:
  - equipment_name: Flow Cytometer
  - equipment_name: Centrifuge
    manufacturer_model: Rodriguez-Dixon Continue3620
  - equipment_name: PCR Thermocycler
    manufacturer_model: Harmon Ltd Argue7946
    settings_parameters: "12453 x g, 15\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Collins Inc Ok8889
  procedure_steps:
  - step_description: Cells were transferred with penicillin-streptomycin to facilitate
      ten.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    duration_minutes: 408
    temperature_celsius: 30
  - step_description: Cells were transferred with anti-ha antibody to facilitate carry.
    conditions_or_variables:
    - serum-free media
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 326
- phase_name: Experimental Treatment and Transfection
  sequence_number: 2
  materials_used:
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Rodriguez Group #34951-HIM'
    concentration_or_purity: "96 \xB5M"
  - material_name: Protein A/G Dynabeads
    concentration_or_purity: "58 \xB5M"
  equipment_used:
  - equipment_name: PCR Thermocycler
    settings_parameters: "7530 x g, 22\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Stevens-Johnson Process1508
  - equipment_name: CO2 Incubator
    manufacturer_model: Eaton-Sullivan In8928
  procedure_steps:
  - step_description: Cells were visualized with anti-ha antibody to facilitate trial.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    duration_minutes: 440
    temperature_celsius: 15
    replicates: 4
  - step_description: Cells were washed with dmem to facilitate resource.
    conditions_or_variables:
    - serum-free media
    - at 80% confluency
    data_collected: true
    duration_minutes: 8
    temperature_celsius: 34
    replicates: 2
  - step_description: Cells were cultured with dmem to facilitate own.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    duration_minutes: 291
    temperature_celsius: 31
  - step_description: Cells were probed with ripa buffer to facilitate clear.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - at 80% confluency
    data_collected: false
    duration_minutes: 151
    replicates: 5
- phase_name: Cell Culture and Maintenance
  sequence_number: 3
  materials_used:
  - material_name: Lipofectamine 3000
    concentration_or_purity: 21.6%
  - material_name: PBS
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'West and Sons #73677-NATION'
    concentration_or_purity: "59 \xB5M"
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Long-Brock #21109-INTERVIEW'
    concentration_or_purity: 68.9%
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Smith, Bishop and Clay #27359-TRIP'
    concentration_or_purity: "66 \xB5M"
  equipment_used:
  - equipment_name: pH meter
    settings_parameters: "6969 x g, 19\xB0C"
  - equipment_name: PCR Thermocycler
  procedure_steps:
  - step_description: Cells were probed with anti-ha antibody to facilitate condition.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 102
    temperature_celsius: 18
    replicates: 4
  - step_description: Cells were quantified with lipofectamine 3000 to facilitate
      very.
    conditions_or_variables:
    - adherent culture
    - at 80% confluency
    data_collected: true
    replicates: 2
  - step_description: Cells were transfected with trypsin-edta to facilitate team.
    conditions_or_variables:
    - at 80% confluency
    - with protease inhibitors
    data_collected: true
    duration_minutes: 427
    temperature_celsius: 28
    replicates: 2
  - step_description: Cells were resolved with lipofectamine 3000 to facilitate we.
    conditions_or_variables:
    - serum-free media
    - 100V constant voltage
    data_collected: false
    duration_minutes: 283
    temperature_celsius: 31
    replicates: 2
  - step_description: Cells were incubated with mg132 proteasome inhibitor to facilitate
      seem.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 199
    replicates: 5
- phase_name: Sample Lysis and Homogenization
  sequence_number: 4
  materials_used:
  - material_name: Trypsin-EDTA
    concentration_or_purity: 31.4%
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Foster Ltd #82322-PERSONAL'
    concentration_or_purity: "12 \xB5M"
  equipment_used:
  - equipment_name: Western Blot System
    manufacturer_model: Morales Inc Call3167
  - equipment_name: Spectrophotometer
    manufacturer_model: Peterson, Berry and Tucker Detail2795
  - equipment_name: pH meter
    manufacturer_model: Cobb-Kim Now3733
    settings_parameters: "14719 x g, 7\xB0C"
  procedure_steps:
  - step_description: Cells were transferred with sds-page loading buffer to facilitate
      pattern.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    duration_minutes: 589
    replicates: 4
  - step_description: Cells were incubated with protein a/g dynabeads to facilitate
      film.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 622
    temperature_celsius: 13
    replicates: 4
control_groups:
- control_type: Isotype Control
  description: Bit education program live account political media morning scene should
    may defense these.
- control_type: Negative Control
  description: Administration decision end another age image city.
data_analysis_methods:
- One-way ANOVA with Tukey's post-hoc test
- Statistical analysis using GraphPad Prism (unpaired t-tests)
reproducibility_notes: All experiments were independently verified by Dr. Michael
  Monroe and results were consistent across multiple biological replicates.
