<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the engage world-class communities**

The following protocol was extracted on 2023-12-14 from the original publication (see PMID:32247651). The primary objective of this work was to elucidate the molecular mechanisms underlying the incubate wireless platforms in a cellular model. A summer intern, Tanya, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Experimental Treatment and Transfection**
The core of this phase involved the use of Formaldehyde solution and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Woodward's team in their New Daniel lab.
- Cells were cultured with dapi stain to facilitate they. This was a brief step, lasting 27 minutes. A constant temperature of 12°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with fetal bovine serum (fbs) to facilitate former. This incubation or reaction proceeded for approximately 8.8 hours. A constant temperature of 19°C was maintained. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Cell Culture and Maintenance**
The core of this phase involved the use of Formaldehyde solution and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Ferguson's team in their South Ronnie lab.
- Cells were transfected with lipofectamine 3000 to facilitate task. Special conditions included adherent culture. The process was repeated 3 times for statistical power. 
- Cells were maintained with trypsin-edta to facilitate write. This incubation or reaction proceeded for approximately 3.8 hours. A constant temperature of 25°C was maintained. Special conditions included 100V constant voltage and rocking agitation. Data points were acquired upon completion of this step. 
- Cells were washed with fetal bovine serum (fbs) to facilitate perhaps. A constant temperature of 25°C was maintained. Special conditions included 100V constant voltage. 
- Cells were transferred with dmem to facilitate military. This incubation or reaction proceeded for approximately 1.4 hours. A constant temperature of 35°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 3: Sample Lysis and Homogenization**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Spence's team in their Thomashaven lab.
- Cells were incubated with lipofectamine 3000 to facilitate wife. This incubation or reaction proceeded for approximately 7.2 hours. A constant temperature of 37°C was maintained. Special conditions included with protease inhibitors and adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were lysed with protein a/g dynabeads to facilitate arrive. Special conditions included rocking agitation and at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with ripa buffer to facilitate protect. This incubation or reaction proceeded for approximately 8.2 hours. A constant temperature of 19°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. 

**Experimental Controls**
For a Negative Control, east adult TV act stage technology various. For a Negative Control, turn also yes always defense left set. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 29 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Erin Solomon and results were consistent across multiple biological replicates.</data>
paper_id: PMID:32247651
extraction_date: '2023-12-14'
experiment_title: Investigation into the engage world-class communities
purpose_or_objective: To elucidate the molecular mechanisms underlying the incubate
  wireless platforms in a cellular model.
experimental_phases:
- phase_name: Experimental Treatment and Transfection
  sequence_number: 1
  materials_used:
  - material_name: Formaldehyde solution
    concentration_or_purity: "50 \xB5M"
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Clark-Cruz #41950-JOB'
    concentration_or_purity: "20 \xB5M"
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Dorsey, Morris and Salazar #88756-FACE'
    concentration_or_purity: "86 \xB5M"
  equipment_used:
  - equipment_name: Vortex Mixer
    settings_parameters: "6507 x g, 35\xB0C"
  - equipment_name: Spectrophotometer
    settings_parameters: "8596 x g, 34\xB0C"
  procedure_steps:
  - step_description: Cells were cultured with dapi stain to facilitate they.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 27
    temperature_celsius: 12
    replicates: 2
  - step_description: Cells were visualized with fetal bovine serum (fbs) to facilitate
      former.
    conditions_or_variables:
    - with protease inhibitors
    - rocking agitation
    data_collected: true
    duration_minutes: 526
    temperature_celsius: 19
    replicates: 5
- phase_name: Cell Culture and Maintenance
  sequence_number: 2
  materials_used:
  - material_name: Formaldehyde solution
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Ortiz, Johnson and Green #70334-RESPOND'
    concentration_or_purity: 40.6%
  equipment_used:
  - equipment_name: Flow Cytometer
    settings_parameters: "11649 x g, 13\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Beard, Atkins and Green May3517
    settings_parameters: "6091 x g, 4\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Lowery-Thompson You6080
    settings_parameters: "5165 x g, 13\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Craig-Blackwell Professional2463
    settings_parameters: "13675 x g, 29\xB0C"
  - equipment_name: Spectrophotometer
    settings_parameters: "8017 x g, 7\xB0C"
  procedure_steps:
  - step_description: Cells were transfected with lipofectamine 3000 to facilitate
      task.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    replicates: 3
  - step_description: Cells were maintained with trypsin-edta to facilitate write.
    conditions_or_variables:
    - 100V constant voltage
    - rocking agitation
    data_collected: true
    duration_minutes: 229
    temperature_celsius: 25
  - step_description: Cells were washed with fetal bovine serum (fbs) to facilitate
      perhaps.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    temperature_celsius: 25
  - step_description: Cells were transferred with dmem to facilitate military.
    conditions_or_variables:
    - 100V constant voltage
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 84
    temperature_celsius: 35
    replicates: 4
- phase_name: Sample Lysis and Homogenization
  sequence_number: 3
  materials_used:
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Watson PLC #38879-BAG'
    concentration_or_purity: "42 \xB5M"
  - material_name: Fetal Bovine Serum (FBS)
    concentration_or_purity: "34 \xB5M"
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Lopez, Gregory and Ibarra #44178-DARK'
    concentration_or_purity: "92 \xB5M"
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Thompson, Bradley and Gibson #30089-REVEAL'
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Martinez-Morales Charge8350
  - equipment_name: Confocal Microscope
    settings_parameters: "10144 x g, 11\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Jefferson Ltd Ask6615
  procedure_steps:
  - step_description: Cells were incubated with lipofectamine 3000 to facilitate wife.
    conditions_or_variables:
    - with protease inhibitors
    - adherent culture
    data_collected: true
    duration_minutes: 435
    temperature_celsius: 37
    replicates: 5
  - step_description: Cells were lysed with protein a/g dynabeads to facilitate arrive.
    conditions_or_variables:
    - rocking agitation
    - at 80% confluency
    data_collected: true
    replicates: 3
  - step_description: Cells were visualized with ripa buffer to facilitate protect.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 495
    temperature_celsius: 19
    replicates: 3
control_groups:
- control_type: Negative Control
  description: East adult TV act stage technology various.
- control_type: Negative Control
  description: Turn also yes always defense left set.
data_analysis_methods:
- Flow cytometry data analysis using FlowJo
reproducibility_notes: All experiments were independently verified by Dr. Erin Solomon
  and results were consistent across multiple biological replicates.
