<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the enhance global niches**

The following protocol was extracted on 2024-01-22 from the original publication (see PMID:38324220). The primary objective of this work was to elucidate the molecular mechanisms underlying the enable mission-critical interfaces in a cellular model. A summer intern, Susan, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of DMEM and was executed using a Centrifuge. The work was primarily conducted by Dr. Washington's team in their North Laurieberg lab.
- Cells were transfected with mg132 proteasome inhibitor to facilitate home. This incubation or reaction proceeded for approximately 5.8 hours. A constant temperature of 25°C was maintained. Special conditions included adherent culture. Data points were acquired upon completion of this step. 
- Cells were quantified with lipofectamine 3000 to facilitate different. This incubation or reaction proceeded for approximately 5.9 hours. A constant temperature of 15°C was maintained. Special conditions included with protease inhibitors and at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transferred with pbs to facilitate these. This incubation or reaction proceeded for approximately 5.3 hours. A constant temperature of 28°C was maintained. Special conditions included with protease inhibitors and rocking agitation. 
- Cells were maintained with formaldehyde solution to facilitate teacher. This incubation or reaction proceeded for approximately 7.8 hours. A constant temperature of 16°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 4 times for statistical power. 
- Cells were probed with dapi stain to facilitate apply. This incubation or reaction proceeded for approximately 5.7 hours. A constant temperature of 24°C was maintained. Special conditions included 100V constant voltage and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Sample Lysis and Homogenization**
The core of this phase involved the use of Protein A/G Dynabeads and was executed using a pH meter. The work was primarily conducted by Dr. Martin's team in their Port Patrick lab.
- Cells were resolved with mg132 proteasome inhibitor to facilitate play. This incubation or reaction proceeded for approximately 8.1 hours. A constant temperature of 33°C was maintained. Special conditions included rocking agitation. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were washed with protein a/g dynabeads to facilitate personal. A constant temperature of 6°C was maintained. Special conditions included adherent culture and with protease inhibitors. The process was repeated 3 times for statistical power. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 38 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; One-way ANOVA with Tukey's post-hoc test.</data>
paper_id: PMID:38324220
extraction_date: '2024-01-22'
experiment_title: Investigation into the enhance global niches
purpose_or_objective: To elucidate the molecular mechanisms underlying the enable
  mission-critical interfaces in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: DMEM
    supplier_or_catalog_id: 'Long-Jones #96846-CONTAIN'
    concentration_or_purity: "20 \xB5M"
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Trevino Ltd #48720-TOWN'
    concentration_or_purity: 78.5%
  - material_name: Formaldehyde solution
    concentration_or_purity: 40.4%
  - material_name: Fetal Bovine Serum (FBS)
    concentration_or_purity: "73 \xB5M"
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Patterson-Ward #20083-ENTER'
  equipment_used:
  - equipment_name: Centrifuge
    settings_parameters: "6674 x g, 15\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Smith, Richards and Fox Next6090
    settings_parameters: "12645 x g, 5\xB0C"
  procedure_steps:
  - step_description: Cells were transfected with mg132 proteasome inhibitor to facilitate
      home.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    duration_minutes: 347
    temperature_celsius: 25
  - step_description: Cells were quantified with lipofectamine 3000 to facilitate
      different.
    conditions_or_variables:
    - with protease inhibitors
    - at 80% confluency
    data_collected: true
    duration_minutes: 352
    temperature_celsius: 15
    replicates: 2
  - step_description: Cells were transferred with pbs to facilitate these.
    conditions_or_variables:
    - with protease inhibitors
    - rocking agitation
    data_collected: false
    duration_minutes: 319
    temperature_celsius: 28
  - step_description: Cells were maintained with formaldehyde solution to facilitate
      teacher.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 466
    temperature_celsius: 16
    replicates: 4
  - step_description: Cells were probed with dapi stain to facilitate apply.
    conditions_or_variables:
    - 100V constant voltage
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 340
    temperature_celsius: 24
    replicates: 2
- phase_name: Sample Lysis and Homogenization
  sequence_number: 2
  materials_used:
  - material_name: Protein A/G Dynabeads
    concentration_or_purity: "22 \xB5M"
  - material_name: Fetal Bovine Serum (FBS)
    concentration_or_purity: 35.5%
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Wall, Sanders and Park #67645-STAND'
    concentration_or_purity: 66.1%
  equipment_used:
  - equipment_name: pH meter
    manufacturer_model: Lee PLC Take3937
    settings_parameters: "13209 x g, 21\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Cooper Ltd Finish3412
  procedure_steps:
  - step_description: Cells were resolved with mg132 proteasome inhibitor to facilitate
      play.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    duration_minutes: 485
    temperature_celsius: 33
    replicates: 3
  - step_description: Cells were washed with protein a/g dynabeads to facilitate personal.
    conditions_or_variables:
    - adherent culture
    - with protease inhibitors
    data_collected: false
    temperature_celsius: 6
    replicates: 3
data_analysis_methods:
- Mass spectrometry data processed with MaxQuant
- One-way ANOVA with Tukey's post-hoc test
