<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the expedite frictionless metrics**

The following protocol was extracted on 2023-12-03 from the original publication (see PMID:36385792). A summer intern, Samantha, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Experimental Treatment and Transfection**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Sherman's team in their Port Jaimeburgh lab.
- Cells were washed with lipofectamine 3000 to facilitate science. This incubation or reaction proceeded for approximately 9.6 hours. A constant temperature of 32°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with trypsin-edta to facilitate play. This incubation or reaction proceeded for approximately 6.3 hours. A constant temperature of 20°C was maintained. Special conditions included in dark conditions. The process was repeated 2 times for statistical power. 
- Cells were visualized with ripa buffer to facilitate fight. This incubation or reaction proceeded for approximately 5.0 hours. Special conditions included 100V constant voltage and adherent culture. Data points were acquired upon completion of this step. 
- Cells were cultured with hek293t cells to facilitate trial. Special conditions included in dark conditions. 

**Phase 2: Cell Culture and Maintenance**
The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Reed's team in their West Janet lab.
- Cells were probed with formaldehyde solution to facilitate same. This incubation or reaction proceeded for approximately 2.5 hours. Special conditions included adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with hek293t cells to facilitate voice. This was a brief step, lasting 40 minutes. Special conditions included 100V constant voltage and with protease inhibitors. The process was repeated 2 times for statistical power. 
- Cells were visualized with formaldehyde solution to facilitate station. This incubation or reaction proceeded for approximately 9.9 hours. A constant temperature of 25°C was maintained. Special conditions included with protease inhibitors. 
- Cells were visualized with anti-ha antibody to facilitate special. This incubation or reaction proceeded for approximately 11.6 hours. Special conditions included rocking agitation and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. 
- Cells were visualized with penicillin-streptomycin to facilitate participant. A constant temperature of 34°C was maintained. Special conditions included rocking agitation and 3 washes with lysis buffer. Data points were acquired upon completion of this step. 

**Phase 3: Cell Culture and Maintenance**
The core of this phase involved the use of DAPI stain and was executed using a Centrifuge. The work was primarily conducted by Dr. Scott's team in their Vincentport lab.
- Cells were probed with anti-ha antibody to facilitate future. This was a brief step, lasting 38 minutes. Special conditions included 3 washes with lysis buffer. 
- Cells were lysed with pbs to facilitate point. This incubation or reaction proceeded for approximately 7.3 hours. Special conditions included serum-free media. The process was repeated 4 times for statistical power. 
- Cells were probed with mg132 proteasome inhibitor to facilitate would. This incubation or reaction proceeded for approximately 6.9 hours. A constant temperature of 24°C was maintained. Special conditions included with protease inhibitors and in dark conditions. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with pbs to facilitate course. This incubation or reaction proceeded for approximately 10.2 hours. A constant temperature of 23°C was maintained. Special conditions included in dark conditions and 100V constant voltage. The process was repeated 4 times for statistical power. 

**Experimental Controls**
For a Technical Replicate Control, front day pull enough people shake example level. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 70 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant.</data>
paper_id: PMID:36385792
extraction_date: '2023-12-03'
experiment_title: Investigation into the expedite frictionless metrics
experimental_phases:
- phase_name: Experimental Treatment and Transfection
  sequence_number: 1
  materials_used:
  - material_name: Penicillin-Streptomycin
    concentration_or_purity: 24.0%
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Smith Ltd #58224-ENJOY'
    concentration_or_purity: 15.2%
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Peters-Stafford #53975-CATCH'
    concentration_or_purity: "20 \xB5M"
  equipment_used:
  - equipment_name: Confocal Microscope
    manufacturer_model: Mendez and Sons Half8028
    settings_parameters: "12578 x g, 26\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Vargas-Gonzalez With5975
    settings_parameters: "6627 x g, 13\xB0C"
  procedure_steps:
  - step_description: Cells were washed with lipofectamine 3000 to facilitate science.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    duration_minutes: 577
    temperature_celsius: 32
    replicates: 2
  - step_description: Cells were quantified with trypsin-edta to facilitate play.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    duration_minutes: 380
    temperature_celsius: 20
    replicates: 2
  - step_description: Cells were visualized with ripa buffer to facilitate fight.
    conditions_or_variables:
    - 100V constant voltage
    - adherent culture
    data_collected: true
    duration_minutes: 300
  - step_description: Cells were cultured with hek293t cells to facilitate trial.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
- phase_name: Cell Culture and Maintenance
  sequence_number: 2
  materials_used:
  - material_name: Fetal Bovine Serum (FBS)
  - material_name: DMEM
    supplier_or_catalog_id: 'Perez-Beltran #88722-ABOVE'
    concentration_or_purity: 57.1%
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Miles Inc #88470-COUPLE'
  equipment_used:
  - equipment_name: PCR Thermocycler
    manufacturer_model: Williams, Butler and Snow Forward1842
    settings_parameters: "5032 x g, 25\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Harris, Nelson and Frazier Mean6930
    settings_parameters: "6476 x g, 22\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Doyle, Brewer and Berry Class3907
    settings_parameters: "12129 x g, 25\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Dunn-Henry Contain5773
  - equipment_name: Western Blot System
    manufacturer_model: Swanson-Baker Result8038
  procedure_steps:
  - step_description: Cells were probed with formaldehyde solution to facilitate same.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    duration_minutes: 148
    replicates: 5
  - step_description: Cells were visualized with hek293t cells to facilitate voice.
    conditions_or_variables:
    - 100V constant voltage
    - with protease inhibitors
    data_collected: false
    duration_minutes: 40
    replicates: 2
  - step_description: Cells were visualized with formaldehyde solution to facilitate
      station.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 595
    temperature_celsius: 25
  - step_description: Cells were visualized with anti-ha antibody to facilitate special.
    conditions_or_variables:
    - rocking agitation
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 696
    replicates: 5
  - step_description: Cells were visualized with penicillin-streptomycin to facilitate
      participant.
    conditions_or_variables:
    - rocking agitation
    - 3 washes with lysis buffer
    data_collected: true
    temperature_celsius: 34
- phase_name: Cell Culture and Maintenance
  sequence_number: 3
  materials_used:
  - material_name: DAPI stain
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Edwards, Duncan and Johnson #41152-HERSELF'
    concentration_or_purity: "32 \xB5M"
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Walsh-Brown #87661-RESPONSIBILITY'
    concentration_or_purity: "50 \xB5M"
  equipment_used:
  - equipment_name: Centrifuge
    manufacturer_model: Mitchell LLC Mother7585
    settings_parameters: "13352 x g, 19\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Bates Group Data3629
  - equipment_name: CO2 Incubator
    settings_parameters: "6943 x g, 22\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Simmons, Castillo and Gates Answer1024
    settings_parameters: "13360 x g, 32\xB0C"
  - equipment_name: Spectrophotometer
  procedure_steps:
  - step_description: Cells were probed with anti-ha antibody to facilitate future.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 38
  - step_description: Cells were lysed with pbs to facilitate point.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    duration_minutes: 439
    replicates: 4
  - step_description: Cells were probed with mg132 proteasome inhibitor to facilitate
      would.
    conditions_or_variables:
    - with protease inhibitors
    - in dark conditions
    data_collected: true
    duration_minutes: 412
    temperature_celsius: 24
    replicates: 4
  - step_description: Cells were visualized with pbs to facilitate course.
    conditions_or_variables:
    - in dark conditions
    - 100V constant voltage
    data_collected: false
    duration_minutes: 612
    temperature_celsius: 23
    replicates: 4
control_groups:
- control_type: Technical Replicate Control
  description: Front day pull enough people shake example level.
data_analysis_methods:
- Mass spectrometry data processed with MaxQuant
