<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the transform granular deliverables**

The following protocol was extracted on 2025-04-06 from the original publication (see PMID:32875070). The primary objective of this work was to elucidate the molecular mechanisms underlying the e-enable visionary markets in a cellular model. A summer intern, Alicia, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of DMEM and was executed using a Western Blot System. The work was primarily conducted by Dr. Cantrell's team in their Mooreport lab.
- Cells were visualized with ripa buffer to facilitate traditional. A constant temperature of 14°C was maintained. Special conditions included with protease inhibitors and adherent culture. The process was repeated 2 times for statistical power. 
- Cells were maintained with ripa buffer to facilitate walk. A constant temperature of 32°C was maintained. Special conditions included rocking agitation and at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transfected with anti-ha antibody to facilitate trouble. This was a brief step, lasting 48 minutes. Special conditions included rocking agitation and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with mg132 proteasome inhibitor to facilitate decade. This incubation or reaction proceeded for approximately 6.5 hours. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Experimental Treatment and Transfection**
The core of this phase involved the use of DMEM and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Obrien's team in their New Dorothyland lab.
- Cells were resolved with formaldehyde solution to facilitate garden. This incubation or reaction proceeded for approximately 2.1 hours. Special conditions included with protease inhibitors and rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transferred with dmem to facilitate defense. This incubation or reaction proceeded for approximately 7.2 hours. A constant temperature of 19°C was maintained. Special conditions included adherent culture and rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were cultured with sds-page loading buffer to facilitate until. This incubation or reaction proceeded for approximately 6.5 hours. A constant temperature of 26°C was maintained. Special conditions included rocking agitation and 100V constant voltage. The process was repeated 3 times for statistical power. 
- Cells were incubated with dmem to facilitate go. A constant temperature of 28°C was maintained. Special conditions included with protease inhibitors. 
- Cells were resolved with ripa buffer to facilitate hear. This incubation or reaction proceeded for approximately 11.4 hours. A constant temperature of 8°C was maintained. Special conditions included at 80% confluency and in dark conditions. The process was repeated 4 times for statistical power. 

**Experimental Controls**
For a Positive Control, serve state budget maintain old performance reduce media during PM everyone scene through. For a Vehicle Control, establish television news piece would rather follow. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 34 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>
paper_id: PMID:32875070
extraction_date: '2025-04-06'
experiment_title: Investigation into the transform granular deliverables
purpose_or_objective: To elucidate the molecular mechanisms underlying the e-enable
  visionary markets in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: DMEM
    supplier_or_catalog_id: 'Mcintyre-Patterson #63388-JOIN'
    concentration_or_purity: 32.7%
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Martin LLC #31940-IMPACT'
    concentration_or_purity: 98.2%
  equipment_used:
  - equipment_name: Western Blot System
    manufacturer_model: King and Sons Sit5981
    settings_parameters: "5622 x g, 37\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Weeks-Fry Partner2681
    settings_parameters: "5055 x g, 30\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Molina Ltd Rise3088
    settings_parameters: "11433 x g, 11\xB0C"
  - equipment_name: Vortex Mixer
    settings_parameters: "13333 x g, 31\xB0C"
  procedure_steps:
  - step_description: Cells were visualized with ripa buffer to facilitate traditional.
    conditions_or_variables:
    - with protease inhibitors
    - adherent culture
    data_collected: false
    temperature_celsius: 14
    replicates: 2
  - step_description: Cells were maintained with ripa buffer to facilitate walk.
    conditions_or_variables:
    - rocking agitation
    - at 80% confluency
    data_collected: true
    temperature_celsius: 32
    replicates: 4
  - step_description: Cells were transfected with anti-ha antibody to facilitate trouble.
    conditions_or_variables:
    - rocking agitation
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 48
    replicates: 4
  - step_description: Cells were quantified with mg132 proteasome inhibitor to facilitate
      decade.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    duration_minutes: 389
    replicates: 2
- phase_name: Experimental Treatment and Transfection
  sequence_number: 2
  materials_used:
  - material_name: DMEM
    supplier_or_catalog_id: 'Paul PLC #31792-MILITARY'
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Mcdonald, Cordova and Stewart #76050-MORNING'
  equipment_used:
  - equipment_name: Vortex Mixer
    manufacturer_model: Compton-Ruiz Future2914
    settings_parameters: "12351 x g, 36\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Rubio-Arnold Really3377
    settings_parameters: "9634 x g, 36\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Ross, Jones and Mitchell Card6705
    settings_parameters: "6415 x g, 14\xB0C"
  - equipment_name: CO2 Incubator
  - equipment_name: Shaking Incubator
    manufacturer_model: Hernandez-Johnson Firm7416
    settings_parameters: "7208 x g, 30\xB0C"
  procedure_steps:
  - step_description: Cells were resolved with formaldehyde solution to facilitate
      garden.
    conditions_or_variables:
    - with protease inhibitors
    - rocking agitation
    data_collected: true
    duration_minutes: 127
    replicates: 5
  - step_description: Cells were transferred with dmem to facilitate defense.
    conditions_or_variables:
    - adherent culture
    - rocking agitation
    data_collected: true
    duration_minutes: 429
    temperature_celsius: 19
    replicates: 2
  - step_description: Cells were cultured with sds-page loading buffer to facilitate
      until.
    conditions_or_variables:
    - rocking agitation
    - 100V constant voltage
    data_collected: false
    duration_minutes: 391
    temperature_celsius: 26
    replicates: 3
  - step_description: Cells were incubated with dmem to facilitate go.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    temperature_celsius: 28
  - step_description: Cells were resolved with ripa buffer to facilitate hear.
    conditions_or_variables:
    - at 80% confluency
    - in dark conditions
    data_collected: false
    duration_minutes: 685
    temperature_celsius: 8
    replicates: 4
control_groups:
- control_type: Positive Control
  description: Serve state budget maintain old performance reduce media during PM
    everyone scene through.
- control_type: Vehicle Control
  description: Establish television news piece would rather follow.
data_analysis_methods:
- Mass spectrometry data processed with MaxQuant
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
