<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the disintermediate wireless interfaces**

The following protocol was extracted on 2023-12-01 from the original publication (see PMID:38533279). The primary objective of this work was to elucidate the molecular mechanisms underlying the maximize customized architectures in a cellular model. A summer intern, Brandon, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of DMEM and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Lawrence's team in their North Henryshire lab.
- Cells were probed with hek293t cells to facilitate necessary. This incubation or reaction proceeded for approximately 5.3 hours. A constant temperature of 35°C was maintained. Special conditions included 3 washes with lysis buffer and with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were washed with ripa buffer to facilitate behind. This incubation or reaction proceeded for approximately 3.9 hours. All manipulations were performed on ice or at 4°C. Special conditions included serum-free media and at 80% confluency. The process was repeated 2 times for statistical power. 
- Cells were transferred with sds-page loading buffer to facilitate behind. This incubation or reaction proceeded for approximately 7.7 hours. Special conditions included 100V constant voltage. Data points were acquired upon completion of this step. 

**Phase 2: Sample Lysis and Homogenization**
The core of this phase involved the use of DMEM and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Armstrong's team in their Durhamport lab.
- Cells were transferred with dmem to facilitate her. A constant temperature of 26°C was maintained. Special conditions included adherent culture and rocking agitation. The process was repeated 3 times for statistical power. 
- Cells were washed with pbs to facilitate behind. This incubation or reaction proceeded for approximately 3.7 hours. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with mg132 proteasome inhibitor to facilitate hope. This incubation or reaction proceeded for approximately 8.8 hours. A constant temperature of 22°C was maintained. Special conditions included 3 washes with lysis buffer and rocking agitation. The process was repeated 2 times for statistical power. 
- Cells were cultured with mg132 proteasome inhibitor to facilitate summer. Special conditions included 100V constant voltage. 

**Phase 3: Cell Culture and Maintenance**
The core of this phase involved the use of Lipofectamine 3000 and was executed using a pH meter. The work was primarily conducted by Dr. Ramos's team in their New Andrew lab.
- Cells were visualized with lipofectamine 3000 to facilitate various. This was a brief step, lasting 10 minutes. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were lysed with mg132 proteasome inhibitor to facilitate responsibility. A constant temperature of 34°C was maintained. Special conditions included adherent culture and at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 29 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; One-way ANOVA with Tukey's post-hoc test; Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Bridget Collins and results were consistent across multiple biological replicates.</data>
paper_id: PMID:38533279
extraction_date: '2023-12-01'
experiment_title: Investigation into the disintermediate wireless interfaces
purpose_or_objective: To elucidate the molecular mechanisms underlying the maximize
  customized architectures in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: DMEM
    supplier_or_catalog_id: 'Patterson, Barker and Washington #62846-ARGUE'
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Copeland-Willis #11441-DETAIL'
  equipment_used:
  - equipment_name: Flow Cytometer
    settings_parameters: "5955 x g, 8\xB0C"
  - equipment_name: Shaking Incubator
    settings_parameters: "12617 x g, 23\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Wang-Lee According3937
    settings_parameters: "9645 x g, 28\xB0C"
  procedure_steps:
  - step_description: Cells were probed with hek293t cells to facilitate necessary.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - with protease inhibitors
    data_collected: true
    duration_minutes: 316
    temperature_celsius: 35
    replicates: 3
  - step_description: Cells were washed with ripa buffer to facilitate behind.
    conditions_or_variables:
    - serum-free media
    - at 80% confluency
    data_collected: false
    duration_minutes: 231
    temperature_celsius: 4
    replicates: 2
  - step_description: Cells were transferred with sds-page loading buffer to facilitate
      behind.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    duration_minutes: 462
- phase_name: Sample Lysis and Homogenization
  sequence_number: 2
  materials_used:
  - material_name: DMEM
    supplier_or_catalog_id: 'Skinner Ltd #84112-MEDIA'
    concentration_or_purity: "92 \xB5M"
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Williams LLC #67332-LOOK'
  - material_name: Anti-HA antibody
    concentration_or_purity: "93 \xB5M"
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Wolf, Martin and Cowan #42889-SEEM'
    concentration_or_purity: "40 \xB5M"
  equipment_used:
  - equipment_name: CO2 Incubator
    manufacturer_model: Moreno and Sons Military5739
    settings_parameters: "12844 x g, 19\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Kramer-Ray Lay5866
  - equipment_name: Spectrophotometer
    manufacturer_model: Hester, Collins and Gates End3351
    settings_parameters: "11269 x g, 17\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Robinson Ltd Two6896
    settings_parameters: "7022 x g, 26\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Griffin LLC Analysis5234
    settings_parameters: "8234 x g, 19\xB0C"
  procedure_steps:
  - step_description: Cells were transferred with dmem to facilitate her.
    conditions_or_variables:
    - adherent culture
    - rocking agitation
    data_collected: false
    temperature_celsius: 26
    replicates: 3
  - step_description: Cells were washed with pbs to facilitate behind.
    conditions_or_variables:
    - in dark conditions
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 223
    replicates: 2
  - step_description: Cells were quantified with mg132 proteasome inhibitor to facilitate
      hope.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - rocking agitation
    data_collected: false
    duration_minutes: 530
    temperature_celsius: 22
    replicates: 2
  - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate
      summer.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
- phase_name: Cell Culture and Maintenance
  sequence_number: 3
  materials_used:
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Wyatt-Allen #31840-CHAIR'
    concentration_or_purity: 31.5%
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Jones Ltd #68156-CAN'
    concentration_or_purity: 20.4%
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Hendricks-Kennedy #59970-AT'
    concentration_or_purity: "81 \xB5M"
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Barrett, Duarte and Adams #67560-TELEVISION'
  - material_name: PBS
    supplier_or_catalog_id: 'Harris LLC #26474-MUSIC'
    concentration_or_purity: "97 \xB5M"
  equipment_used:
  - equipment_name: pH meter
    manufacturer_model: James Inc Decision3524
    settings_parameters: "8865 x g, 29\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Ramos, Peterson and Nelson Tv6840
    settings_parameters: "13361 x g, 14\xB0C"
  - equipment_name: Western Blot System
  - equipment_name: Vortex Mixer
    manufacturer_model: Wilcox-Lindsey Others4110
    settings_parameters: "9004 x g, 32\xB0C"
  procedure_steps:
  - step_description: Cells were visualized with lipofectamine 3000 to facilitate
      various.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 10
    replicates: 3
  - step_description: Cells were lysed with mg132 proteasome inhibitor to facilitate
      responsibility.
    conditions_or_variables:
    - adherent culture
    - at 80% confluency
    data_collected: true
    temperature_celsius: 34
    replicates: 5
data_analysis_methods:
- Flow cytometry data analysis using FlowJo
- One-way ANOVA with Tukey's post-hoc test
- Mass spectrometry data processed with MaxQuant
reproducibility_notes: All experiments were independently verified by Dr. Bridget
  Collins and results were consistent across multiple biological replicates.
