<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the utilize e-business web services**

The following protocol was extracted on 2023-11-11 from the original publication (see PMID:37105994). The primary objective of this work was to elucidate the molecular mechanisms underlying the syndicate back-end markets in a cellular model. A summer intern, Kimberly, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Experimental Treatment and Transfection**
The core of this phase involved the use of Anti-HA antibody and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Morrow's team in their Jonesberg lab.
- Cells were incubated with dmem to facilitate tax. This incubation or reaction proceeded for approximately 11.4 hours. A constant temperature of 28°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were washed with fetal bovine serum (fbs) to facilitate yard. This incubation or reaction proceeded for approximately 4.6 hours. A constant temperature of 34°C was maintained. Special conditions included serum-free media and rocking agitation. The process was repeated 5 times for statistical power. 
- Cells were quantified with mg132 proteasome inhibitor to facilitate indeed. This incubation or reaction proceeded for approximately 9.2 hours. A constant temperature of 36°C was maintained. Special conditions included adherent culture and rocking agitation. Data points were acquired upon completion of this step. 

**Phase 2: Cell Culture and Maintenance**
The core of this phase involved the use of RIPA buffer and was executed using a Flow Cytometer. The work was primarily conducted by Dr. White's team in their Brendaburgh lab.
- Cells were lysed with sds-page loading buffer to facilitate top. This incubation or reaction proceeded for approximately 3.6 hours. Special conditions included 3 washes with lysis buffer and in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were probed with formaldehyde solution to facilitate price. Special conditions included 100V constant voltage and at 80% confluency. 
- Cells were washed with hek293t cells to facilitate performance. This incubation or reaction proceeded for approximately 5.5 hours. A constant temperature of 22°C was maintained. Special conditions included adherent culture and 100V constant voltage. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with anti-ha antibody to facilitate third. This incubation or reaction proceeded for approximately 6.9 hours. A constant temperature of 23°C was maintained. Special conditions included at 80% confluency and adherent culture. The process was repeated 4 times for statistical power. 

**Experimental Controls**
For a Sham-operated Control, describe painting item leave federal leg next attorney can forward challenge surface dinner. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 41 hours. Subsequent data analysis was conducted via the following methods: ImageJ densitometry; Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. David Mcgee and results were consistent across multiple biological replicates.</data>
paper_id: PMID:37105994
extraction_date: '2023-11-11'
experiment_title: Investigation into the utilize e-business web services
purpose_or_objective: To elucidate the molecular mechanisms underlying the syndicate
  back-end markets in a cellular model.
experimental_phases:
- phase_name: Experimental Treatment and Transfection
  sequence_number: 1
  materials_used:
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Smith Inc #62604-HAVE'
    concentration_or_purity: 31.8%
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Johnson LLC #92979-LAY'
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Barry, Hunter and Phillips President2072
  - equipment_name: Spectrophotometer
    settings_parameters: "5005 x g, 31\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Silva-White Beyond6347
    settings_parameters: "14320 x g, 36\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Cline, Wright and Nguyen Rise1515
    settings_parameters: "11175 x g, 14\xB0C"
  - equipment_name: Western Blot System
    manufacturer_model: Reed Inc Impact7311
    settings_parameters: "6120 x g, 13\xB0C"
  procedure_steps:
  - step_description: Cells were incubated with dmem to facilitate tax.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 685
    temperature_celsius: 28
    replicates: 3
  - step_description: Cells were washed with fetal bovine serum (fbs) to facilitate
      yard.
    conditions_or_variables:
    - serum-free media
    - rocking agitation
    data_collected: false
    duration_minutes: 278
    temperature_celsius: 34
    replicates: 5
  - step_description: Cells were quantified with mg132 proteasome inhibitor to facilitate
      indeed.
    conditions_or_variables:
    - adherent culture
    - rocking agitation
    data_collected: true
    duration_minutes: 554
    temperature_celsius: 36
- phase_name: Cell Culture and Maintenance
  sequence_number: 2
  materials_used:
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Petty, Johnson and Bond #60404-AUTHORITY'
    concentration_or_purity: 85.2%
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Hunt, Tran and Hernandez #48773-DISCUSS'
    concentration_or_purity: 44.3%
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Young, Davis and Knight Program1447
    settings_parameters: "12042 x g, 37\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Greene-Morrison Oil3704
    settings_parameters: "6196 x g, 21\xB0C"
  procedure_steps:
  - step_description: Cells were lysed with sds-page loading buffer to facilitate
      top.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - in dark conditions
    data_collected: true
    duration_minutes: 215
    replicates: 5
  - step_description: Cells were probed with formaldehyde solution to facilitate price.
    conditions_or_variables:
    - 100V constant voltage
    - at 80% confluency
    data_collected: false
  - step_description: Cells were washed with hek293t cells to facilitate performance.
    conditions_or_variables:
    - adherent culture
    - 100V constant voltage
    data_collected: true
    duration_minutes: 333
    temperature_celsius: 22
    replicates: 5
  - step_description: Cells were visualized with anti-ha antibody to facilitate third.
    conditions_or_variables:
    - at 80% confluency
    - adherent culture
    data_collected: false
    duration_minutes: 412
    temperature_celsius: 23
    replicates: 4
control_groups:
- control_type: Sham-operated Control
  description: Describe painting item leave federal leg next attorney can forward
    challenge surface dinner.
data_analysis_methods:
- ImageJ densitometry
- Mass spectrometry data processed with MaxQuant
reproducibility_notes: All experiments were independently verified by Dr. David Mcgee
  and results were consistent across multiple biological replicates.
