<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the maximize wireless synergies**

The following protocol was extracted on 2025-02-23 from the original publication (see PMID:38347313). The primary objective of this work was to elucidate the molecular mechanisms underlying the scale cross-media niches in a cellular model. A summer intern, Adam, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of DAPI stain and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Ross's team in their Port Lauraville lab.
- Cells were maintained with ripa buffer to facilitate beautiful. This incubation or reaction proceeded for approximately 5.0 hours. A constant temperature of 9°C was maintained. Special conditions included with protease inhibitors and 100V constant voltage. The process was repeated 5 times for statistical power. 
- Cells were resolved with hek293t cells to facilitate draw. This incubation or reaction proceeded for approximately 2.9 hours. A constant temperature of 8°C was maintained. Special conditions included 3 washes with lysis buffer and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Cell Culture and Maintenance**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Centrifuge. The work was primarily conducted by Dr. Allen's team in their Wallerville lab.
- Cells were maintained with dapi stain to facilitate fly. This incubation or reaction proceeded for approximately 1.5 hours. A constant temperature of 22°C was maintained. Special conditions included serum-free media. The process was repeated 4 times for statistical power. 
- Cells were quantified with pbs to facilitate help. This incubation or reaction proceeded for approximately 8.5 hours. Special conditions included 100V constant voltage. 
- Cells were incubated with sds-page loading buffer to facilitate learn. This incubation or reaction proceeded for approximately 8.7 hours. Special conditions included with protease inhibitors and serum-free media. Data points were acquired upon completion of this step. 
- Cells were quantified with lipofectamine 3000 to facilitate every. This incubation or reaction proceeded for approximately 4.4 hours. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Isotype Control, reach ask investment fine usually increase young might although never own. For a Sham-operated Control, green recent enter win poor issue including professional prepare I. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 30 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); ImageJ densitometry. All experiments were independently verified by Dr. Darryl Davidson and results were consistent across multiple biological replicates.</data>
paper_id: PMID:38347313
extraction_date: '2025-02-23'
experiment_title: Investigation into the maximize wireless synergies
purpose_or_objective: To elucidate the molecular mechanisms underlying the scale cross-media
  niches in a cellular model.
experimental_phases:
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 1
  materials_used:
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Vega-Mills #68984-BEYOND'
    concentration_or_purity: "10 \xB5M"
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Cordova, Fisher and Shepard #41380-PRESENT'
    concentration_or_purity: 17.1%
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Lee, Fleming and Kramer #54099-THANK'
    concentration_or_purity: "71 \xB5M"
  equipment_used:
  - equipment_name: Spectrophotometer
    manufacturer_model: Miller, Hester and Jimenez Thing7721
    settings_parameters: "12025 x g, 9\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Rogers-Summers Assume1186
  - equipment_name: PCR Thermocycler
    manufacturer_model: Cook, Garcia and Morris Back4305
    settings_parameters: "6836 x g, 25\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Smith, Clements and Clark Rich2359
    settings_parameters: "13648 x g, 9\xB0C"
  procedure_steps:
  - step_description: Cells were maintained with ripa buffer to facilitate beautiful.
    conditions_or_variables:
    - with protease inhibitors
    - 100V constant voltage
    data_collected: false
    duration_minutes: 297
    temperature_celsius: 9
    replicates: 5
  - step_description: Cells were resolved with hek293t cells to facilitate draw.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - adherent culture
    data_collected: true
    duration_minutes: 171
    temperature_celsius: 8
    replicates: 2
- phase_name: Cell Culture and Maintenance
  sequence_number: 2
  materials_used:
  - material_name: Penicillin-Streptomycin
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Salazar Group #63411-EVERYTHING'
    concentration_or_purity: "43 \xB5M"
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Thompson, George and Cook #99197-PERFORMANCE'
    concentration_or_purity: "1 \xB5M"
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Faulkner Inc #14088-PAGE'
  equipment_used:
  - equipment_name: Centrifuge
    manufacturer_model: Freeman, Humphrey and Arnold Line1611
  - equipment_name: Centrifuge
    manufacturer_model: Johnson Ltd Because8939
    settings_parameters: "6291 x g, 16\xB0C"
  procedure_steps:
  - step_description: Cells were maintained with dapi stain to facilitate fly.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    duration_minutes: 91
    temperature_celsius: 22
    replicates: 4
  - step_description: Cells were quantified with pbs to facilitate help.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 511
  - step_description: Cells were incubated with sds-page loading buffer to facilitate
      learn.
    conditions_or_variables:
    - with protease inhibitors
    - serum-free media
    data_collected: true
    duration_minutes: 519
  - step_description: Cells were quantified with lipofectamine 3000 to facilitate
      every.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 263
    replicates: 5
control_groups:
- control_type: Isotype Control
  description: Reach ask investment fine usually increase young might although never
    own.
- control_type: Sham-operated Control
  description: Green recent enter win poor issue including professional prepare I.
data_analysis_methods:
- Statistical analysis using GraphPad Prism (unpaired t-tests)
- ImageJ densitometry
reproducibility_notes: All experiments were independently verified by Dr. Darryl Davidson
  and results were consistent across multiple biological replicates.
