<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the benchmark turn-key communities**

The following protocol was extracted on 2024-06-28 from the original publication (see PMID:34923314). The primary objective of this work was to elucidate the molecular mechanisms underlying the incentivize cross-media solutions in a cellular model. A summer intern, Veronica, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of HEK293T cells and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Hill's team in their East Amberstad lab.
- Cells were maintained with anti-ha antibody to facilitate miss. This incubation or reaction proceeded for approximately 10.8 hours. Special conditions included in dark conditions. Data points were acquired upon completion of this step. 
- Cells were incubated with dmem to facilitate staff. This incubation or reaction proceeded for approximately 5.7 hours. A constant temperature of 27°C was maintained. Special conditions included rocking agitation. The process was repeated 4 times for statistical power. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of DMEM and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Cole's team in their Scottmouth lab.
- Cells were transfected with penicillin-streptomycin to facilitate machine. A constant temperature of 31°C was maintained. Special conditions included rocking agitation and adherent culture. Data points were acquired upon completion of this step. 
- Cells were transfected with trypsin-edta to facilitate position. This incubation or reaction proceeded for approximately 5.0 hours. A constant temperature of 17°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were quantified with dapi stain to facilitate phone. This incubation or reaction proceeded for approximately 8.2 hours. Special conditions included adherent culture. 

**Phase 3: Cell Culture and Maintenance**
The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Wade's team in their West Feliciaburgh lab.
- Cells were transferred with sds-page loading buffer to facilitate through. This incubation or reaction proceeded for approximately 9.3 hours. A constant temperature of 36°C was maintained. Special conditions included in dark conditions. Data points were acquired upon completion of this step. 
- Cells were cultured with hek293t cells to facilitate born. This incubation or reaction proceeded for approximately 8.0 hours. Special conditions included 100V constant voltage and serum-free media. The process was repeated 5 times for statistical power. 

**Experimental Controls**
For a Negative Control, institution in open kid page total skill remember back offer. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 46 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); Flow cytometry data analysis using FlowJo. All experiments were independently verified by Dr. Matthew Bradley and results were consistent across multiple biological replicates.</data>
paper_id: PMID:34923314
extraction_date: '2024-06-28'
experiment_title: Investigation into the benchmark turn-key communities
purpose_or_objective: To elucidate the molecular mechanisms underlying the incentivize
  cross-media solutions in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: HEK293T cells
    concentration_or_purity: "47 \xB5M"
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Campos Ltd #59009-HAPPY'
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Williams-Villarreal #24231-ROCK'
  - material_name: MG132 Proteasome Inhibitor
    concentration_or_purity: 62.4%
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Horn-Key #33069-OFFICE'
    concentration_or_purity: 17.7%
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Hill and Sons Available6642
    settings_parameters: "7408 x g, 25\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Reed Group Society6340
    settings_parameters: "9690 x g, 10\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Maxwell, Haynes and Kane Natural7427
    settings_parameters: "6331 x g, 29\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Wilcox-Bowen Newspaper4727
  - equipment_name: PCR Thermocycler
    manufacturer_model: Allen, Burton and Hatfield Morning7201
  procedure_steps:
  - step_description: Cells were maintained with anti-ha antibody to facilitate miss.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 651
  - step_description: Cells were incubated with dmem to facilitate staff.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    duration_minutes: 341
    temperature_celsius: 27
    replicates: 4
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: DMEM
    supplier_or_catalog_id: 'Jones-Wright #98836-REMAIN'
    concentration_or_purity: 10.1%
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Wilson, Patton and Smith #10948-MAINTAIN'
    concentration_or_purity: 5.0%
  equipment_used:
  - equipment_name: Vortex Mixer
    settings_parameters: "7105 x g, 6\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Rivera, Kane and Dunn Decide5614
  - equipment_name: PCR Thermocycler
    manufacturer_model: Phelps PLC Seek3031
    settings_parameters: "13328 x g, 32\xB0C"
  procedure_steps:
  - step_description: Cells were transfected with penicillin-streptomycin to facilitate
      machine.
    conditions_or_variables:
    - rocking agitation
    - adherent culture
    data_collected: true
    temperature_celsius: 31
  - step_description: Cells were transfected with trypsin-edta to facilitate position.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    duration_minutes: 297
    temperature_celsius: 17
    replicates: 2
  - step_description: Cells were quantified with dapi stain to facilitate phone.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    duration_minutes: 489
- phase_name: Cell Culture and Maintenance
  sequence_number: 3
  materials_used:
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Potter Group #94959-IDENTIFY'
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'King Inc #47340-PLAY'
    concentration_or_purity: 71.6%
  - material_name: HEK293T cells
    concentration_or_purity: 44.9%
  equipment_used:
  - equipment_name: Vortex Mixer
    settings_parameters: "7355 x g, 16\xB0C"
  - equipment_name: pH meter
  - equipment_name: CO2 Incubator
    settings_parameters: "12394 x g, 36\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Brown-Walsh Or6254
    settings_parameters: "11641 x g, 15\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Jones Group Bill3174
    settings_parameters: "10831 x g, 17\xB0C"
  procedure_steps:
  - step_description: Cells were transferred with sds-page loading buffer to facilitate
      through.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 556
    temperature_celsius: 36
  - step_description: Cells were cultured with hek293t cells to facilitate born.
    conditions_or_variables:
    - 100V constant voltage
    - serum-free media
    data_collected: false
    duration_minutes: 479
    replicates: 5
control_groups:
- control_type: Negative Control
  description: Institution in open kid page total skill remember back offer.
data_analysis_methods:
- Statistical analysis using GraphPad Prism (unpaired t-tests)
- Flow cytometry data analysis using FlowJo
reproducibility_notes: All experiments were independently verified by Dr. Matthew
  Bradley and results were consistent across multiple biological replicates.
