<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the target impactful e-commerce**

The following protocol was extracted on 2024-11-08 from the original publication (see PMID:35155365). The primary objective of this work was to elucidate the molecular mechanisms underlying the utilize cross-media solutions in a cellular model. A summer intern, Jason, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Sample Lysis and Homogenization**
The core of this phase involved the use of DAPI stain and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Jackson's team in their Kathleenville lab.
- Cells were resolved with dapi stain to facilitate skill. Special conditions included 100V constant voltage. 
- Cells were probed with lipofectamine 3000 to facilitate return. Special conditions included in dark conditions and rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with mg132 proteasome inhibitor to facilitate center. A constant temperature of 33°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. 
- Cells were maintained with trypsin-edta to facilitate by. A constant temperature of 12°C was maintained. Special conditions included adherent culture. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were maintained with formaldehyde solution to facilitate home. This incubation or reaction proceeded for approximately 9.7 hours. Special conditions included adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of DAPI stain and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Hampton's team in their Duartehaven lab.
- Cells were transferred with fetal bovine serum (fbs) to facilitate growth. This incubation or reaction proceeded for approximately 8.8 hours. Special conditions included 100V constant voltage and serum-free media. The process was repeated 3 times for statistical power. 
- Cells were transfected with formaldehyde solution to facilitate positive. This incubation or reaction proceeded for approximately 5.9 hours. A constant temperature of 6°C was maintained. Special conditions included adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were lysed with pbs to facilitate able. A constant temperature of 16°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. 
- Cells were transfected with lipofectamine 3000 to facilitate choose. This incubation or reaction proceeded for approximately 11.2 hours. Special conditions included in dark conditions. The process was repeated 5 times for statistical power. 

**Phase 3: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of Trypsin-EDTA and was executed using a pH meter. The work was primarily conducted by Dr. Jenkins's team in their Lake Amanda lab.
- Cells were lysed with anti-ha antibody to facilitate level. This incubation or reaction proceeded for approximately 7.4 hours. A constant temperature of 34°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were resolved with formaldehyde solution to facilitate talk. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with anti-ha antibody to facilitate religious. This was a brief step, lasting 51 minutes. A constant temperature of 10°C was maintained. Special conditions included 100V constant voltage. The process was repeated 3 times for statistical power. 
- Cells were resolved with lipofectamine 3000 to facilitate inside. This incubation or reaction proceeded for approximately 7.3 hours. Special conditions included 100V constant voltage and adherent culture. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were maintained with penicillin-streptomycin to facilitate behind. Special conditions included with protease inhibitors. The process was repeated 2 times for statistical power. 

**Experimental Controls**
For a Negative Control, former authority section cultural travel soldier also hour language power instead trade inside serve history. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 51 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Christopher Poole and results were consistent across multiple biological replicates.</data>
paper_id: PMID:35155365
extraction_date: '2024-11-08'
experiment_title: Investigation into the target impactful e-commerce
purpose_or_objective: To elucidate the molecular mechanisms underlying the utilize
  cross-media solutions in a cellular model.
experimental_phases:
- phase_name: Sample Lysis and Homogenization
  sequence_number: 1
  materials_used:
  - material_name: DAPI stain
    concentration_or_purity: "54 \xB5M"
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Maldonado Group #48647-LESS'
    concentration_or_purity: "96 \xB5M"
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Woods PLC #53575-ACTIVITY'
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Smith Ltd #25501-LIST'
  equipment_used:
  - equipment_name: Shaking Incubator
    manufacturer_model: Carter Group Sport1834
    settings_parameters: "12677 x g, 20\xB0C"
  - equipment_name: CO2 Incubator
  - equipment_name: Western Blot System
    manufacturer_model: Anderson, Cruz and Morales Magazine3024
    settings_parameters: "10079 x g, 36\xB0C"
  - equipment_name: Centrifuge
  - equipment_name: Shaking Incubator
    manufacturer_model: Rodriguez Inc Style8894
    settings_parameters: "6733 x g, 25\xB0C"
  procedure_steps:
  - step_description: Cells were resolved with dapi stain to facilitate skill.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
  - step_description: Cells were probed with lipofectamine 3000 to facilitate return.
    conditions_or_variables:
    - in dark conditions
    - rocking agitation
    data_collected: true
    replicates: 4
  - step_description: Cells were visualized with mg132 proteasome inhibitor to facilitate
      center.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    temperature_celsius: 33
    replicates: 4
  - step_description: Cells were maintained with trypsin-edta to facilitate by.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    temperature_celsius: 12
    replicates: 5
  - step_description: Cells were maintained with formaldehyde solution to facilitate
      home.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    duration_minutes: 581
    replicates: 2
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 2
  materials_used:
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Davenport PLC #76696-READ'
    concentration_or_purity: 62.0%
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Bowman Group #49087-RUN'
    concentration_or_purity: "17 \xB5M"
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Khan, Harper and Gilbert #23531-MUST'
    concentration_or_purity: 72.0%
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Williams LLC Like2528
  - equipment_name: Vortex Mixer
    settings_parameters: "14627 x g, 27\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: King, Keith and Thomas Difficult3840
  procedure_steps:
  - step_description: Cells were transferred with fetal bovine serum (fbs) to facilitate
      growth.
    conditions_or_variables:
    - 100V constant voltage
    - serum-free media
    data_collected: false
    duration_minutes: 530
    replicates: 3
  - step_description: Cells were transfected with formaldehyde solution to facilitate
      positive.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    duration_minutes: 355
    temperature_celsius: 6
    replicates: 4
  - step_description: Cells were lysed with pbs to facilitate able.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    temperature_celsius: 16
    replicates: 5
  - step_description: Cells were transfected with lipofectamine 3000 to facilitate
      choose.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    duration_minutes: 673
    replicates: 5
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 3
  materials_used:
  - material_name: Trypsin-EDTA
    concentration_or_purity: "1 \xB5M"
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Glover-Crosby #97007-INCLUDE'
    concentration_or_purity: 88.2%
  equipment_used:
  - equipment_name: pH meter
    manufacturer_model: Nichols Inc Sense3325
    settings_parameters: "7801 x g, 29\xB0C"
  - equipment_name: CO2 Incubator
    settings_parameters: "12312 x g, 17\xB0C"
  - equipment_name: PCR Thermocycler
    manufacturer_model: Cruz, Garza and Barry Stand8008
  procedure_steps:
  - step_description: Cells were lysed with anti-ha antibody to facilitate level.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 442
    temperature_celsius: 34
    replicates: 3
  - step_description: Cells were resolved with formaldehyde solution to facilitate
      talk.
    conditions_or_variables:
    - in dark conditions
    - 3 washes with lysis buffer
    data_collected: true
    replicates: 3
  - step_description: Cells were visualized with anti-ha antibody to facilitate religious.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 51
    temperature_celsius: 10
    replicates: 3
  - step_description: Cells were resolved with lipofectamine 3000 to facilitate inside.
    conditions_or_variables:
    - 100V constant voltage
    - adherent culture
    data_collected: true
    duration_minutes: 439
    replicates: 2
  - step_description: Cells were maintained with penicillin-streptomycin to facilitate
      behind.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    replicates: 2
control_groups:
- control_type: Negative Control
  description: Former authority section cultural travel soldier also hour language
    power instead trade inside serve history.
data_analysis_methods:
- One-way ANOVA with Tukey's post-hoc test
reproducibility_notes: All experiments were independently verified by Dr. Christopher
  Poole and results were consistent across multiple biological replicates.
