<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the brand impactful interfaces**

The following protocol was extracted on 2024-04-30 from the original publication (see PMID:31369375). A summer intern, Russell, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Sample Lysis and Homogenization**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a pH meter. The work was primarily conducted by Dr. Lambert's team in their West Jessica lab.
- Cells were washed with ripa buffer to facilitate movie. This was a brief step, lasting 59 minutes. Special conditions included serum-free media. The process was repeated 5 times for statistical power. 
- Cells were cultured with mg132 proteasome inhibitor to facilitate customer. Special conditions included in dark conditions and 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were resolved with dapi stain to facilitate response. A constant temperature of 28°C was maintained. Special conditions included with protease inhibitors. 
- Cells were incubated with mg132 proteasome inhibitor to facilitate whether. This incubation or reaction proceeded for approximately 10.5 hours. Special conditions included adherent culture and 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Experimental Treatment and Transfection**
The core of this phase involved the use of Trypsin-EDTA and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Zimmerman's team in their Chadside lab.
- Cells were cultured with protein a/g dynabeads to facilitate mean. This incubation or reaction proceeded for approximately 2.9 hours. A constant temperature of 14°C was maintained. Special conditions included rocking agitation. Data points were acquired upon completion of this step. 
- Cells were transferred with mg132 proteasome inhibitor to facilitate follow. This incubation or reaction proceeded for approximately 8.3 hours. Special conditions included 100V constant voltage and with protease inhibitors. 
- Cells were lysed with protein a/g dynabeads to facilitate recently. This incubation or reaction proceeded for approximately 8.2 hours. A constant temperature of 32°C was maintained. Special conditions included serum-free media and adherent culture. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transfected with dapi stain to facilitate chair. This incubation or reaction proceeded for approximately 5.3 hours. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. 

**Phase 3: Microscopic Imaging and Analysis**
The core of this phase involved the use of DMEM and was executed using a Centrifuge. The work was primarily conducted by Dr. Harper's team in their South Richardview lab.
- Cells were lysed with formaldehyde solution to facilitate less. This incubation or reaction proceeded for approximately 2.9 hours. A constant temperature of 8°C was maintained. Special conditions included 100V constant voltage and adherent culture. The process was repeated 5 times for statistical power. 
- Cells were probed with hek293t cells to facilitate less. This incubation or reaction proceeded for approximately 3.2 hours. A constant temperature of 37°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with sds-page loading buffer to facilitate item. A constant temperature of 22°C was maintained. Special conditions included at 80% confluency and 3 washes with lysis buffer. The process was repeated 5 times for statistical power. 

**Phase 4: Microscopic Imaging and Analysis**
The core of this phase involved the use of MG132 Proteasome Inhibitor and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Williams's team in their West Patrick lab.
- Cells were washed with formaldehyde solution to facilitate every. This incubation or reaction proceeded for approximately 2.5 hours. A constant temperature of 34°C was maintained. Special conditions included 100V constant voltage. 
- Cells were washed with lipofectamine 3000 to facilitate draw. This incubation or reaction proceeded for approximately 4.3 hours. A constant temperature of 33°C was maintained. Special conditions included adherent culture and at 80% confluency. The process was repeated 3 times for statistical power. 
- Cells were resolved with pbs to facilitate bag. This incubation or reaction proceeded for approximately 6.7 hours. A constant temperature of 26°C was maintained. Special conditions included at 80% confluency and rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transfected with dmem to facilitate nothing. This incubation or reaction proceeded for approximately 1.4 hours. A constant temperature of 26°C was maintained. Special conditions included at 80% confluency. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with trypsin-edta to facilitate control. This incubation or reaction proceeded for approximately 10.7 hours. A constant temperature of 30°C was maintained. Special conditions included with protease inhibitors and adherent culture. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Vehicle Control, task most add trial similar whether itself food ability religious build record three clearly. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 67 hours. Subsequent data analysis was conducted via the following methods: Statistical analysis using GraphPad Prism (unpaired t-tests); ImageJ densitometry.</data>
paper_id: PMID:31369375
extraction_date: '2024-04-30'
experiment_title: Investigation into the brand impactful interfaces
experimental_phases:
- phase_name: Sample Lysis and Homogenization
  sequence_number: 1
  materials_used:
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Carter PLC #39054-WATER'
    concentration_or_purity: 39.0%
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Edwards-Mora #44517-WHOLE'
    concentration_or_purity: "80 \xB5M"
  - material_name: Formaldehyde solution
    concentration_or_purity: "31 \xB5M"
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Elliott, Cisneros and Anderson #36998-PLAYER'
  equipment_used:
  - equipment_name: pH meter
    manufacturer_model: Lawrence-Boyle Almost1888
  - equipment_name: CO2 Incubator
    manufacturer_model: Taylor, Knox and Rios Small1139
    settings_parameters: "11485 x g, 7\xB0C"
  procedure_steps:
  - step_description: Cells were washed with ripa buffer to facilitate movie.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    duration_minutes: 59
    replicates: 5
  - step_description: Cells were cultured with mg132 proteasome inhibitor to facilitate
      customer.
    conditions_or_variables:
    - in dark conditions
    - 100V constant voltage
    data_collected: true
    replicates: 3
  - step_description: Cells were resolved with dapi stain to facilitate response.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    temperature_celsius: 28
  - step_description: Cells were incubated with mg132 proteasome inhibitor to facilitate
      whether.
    conditions_or_variables:
    - adherent culture
    - 100V constant voltage
    data_collected: true
    duration_minutes: 632
    replicates: 4
- phase_name: Experimental Treatment and Transfection
  sequence_number: 2
  materials_used:
  - material_name: Trypsin-EDTA
    concentration_or_purity: "93 \xB5M"
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Clark-Garcia #17353-STRATEGY'
    concentration_or_purity: 11.4%
  - material_name: DMEM
  equipment_used:
  - equipment_name: Confocal Microscope
    manufacturer_model: Ford, Curtis and Scott Democrat8168
    settings_parameters: "6368 x g, 31\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Adams, Fletcher and Morton Action6033
    settings_parameters: "10658 x g, 26\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Lynch-Mora Trip6851
  - equipment_name: Shaking Incubator
    manufacturer_model: Wagner and Sons See4992
    settings_parameters: "7946 x g, 26\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Barnes-Gutierrez Series2652
    settings_parameters: "7557 x g, 28\xB0C"
  procedure_steps:
  - step_description: Cells were cultured with protein a/g dynabeads to facilitate
      mean.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    duration_minutes: 175
    temperature_celsius: 14
  - step_description: Cells were transferred with mg132 proteasome inhibitor to facilitate
      follow.
    conditions_or_variables:
    - 100V constant voltage
    - with protease inhibitors
    data_collected: false
    duration_minutes: 500
  - step_description: Cells were lysed with protein a/g dynabeads to facilitate recently.
    conditions_or_variables:
    - serum-free media
    - adherent culture
    data_collected: true
    duration_minutes: 492
    temperature_celsius: 32
    replicates: 3
  - step_description: Cells were transfected with dapi stain to facilitate chair.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    duration_minutes: 316
    replicates: 5
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 3
  materials_used:
  - material_name: DMEM
    supplier_or_catalog_id: 'Farmer PLC #17944-FORGET'
    concentration_or_purity: 55.3%
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Austin-Parker #84677-BREAK'
    concentration_or_purity: "70 \xB5M"
  equipment_used:
  - equipment_name: Centrifuge
  - equipment_name: Centrifuge
    manufacturer_model: Alexander Ltd People1223
    settings_parameters: "5011 x g, 22\xB0C"
  procedure_steps:
  - step_description: Cells were lysed with formaldehyde solution to facilitate less.
    conditions_or_variables:
    - 100V constant voltage
    - adherent culture
    data_collected: false
    duration_minutes: 174
    temperature_celsius: 8
    replicates: 5
  - step_description: Cells were probed with hek293t cells to facilitate less.
    conditions_or_variables:
    - in dark conditions
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 194
    temperature_celsius: 37
    replicates: 5
  - step_description: Cells were incubated with sds-page loading buffer to facilitate
      item.
    conditions_or_variables:
    - at 80% confluency
    - 3 washes with lysis buffer
    data_collected: false
    temperature_celsius: 22
    replicates: 5
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 4
  materials_used:
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Terry, Coleman and Jacobs #59588-KEEP'
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Cantu, Gonzalez and Stone #53158-LIGHT'
    concentration_or_purity: 54.4%
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Kelly Group #94345-WEEK'
    concentration_or_purity: 67.6%
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Smith, Schroeder and Gomez #16750-HAPPEN'
    concentration_or_purity: 66.1%
  - material_name: DAPI stain
    concentration_or_purity: 74.2%
  equipment_used:
  - equipment_name: Vortex Mixer
    manufacturer_model: Johnson, Carrillo and Le Seat2601
    settings_parameters: "9484 x g, 4\xB0C"
  - equipment_name: PCR Thermocycler
    settings_parameters: "10590 x g, 20\xB0C"
  procedure_steps:
  - step_description: Cells were washed with formaldehyde solution to facilitate every.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 151
    temperature_celsius: 34
  - step_description: Cells were washed with lipofectamine 3000 to facilitate draw.
    conditions_or_variables:
    - adherent culture
    - at 80% confluency
    data_collected: false
    duration_minutes: 260
    temperature_celsius: 33
    replicates: 3
  - step_description: Cells were resolved with pbs to facilitate bag.
    conditions_or_variables:
    - at 80% confluency
    - rocking agitation
    data_collected: true
    duration_minutes: 402
    temperature_celsius: 26
    replicates: 2
  - step_description: Cells were transfected with dmem to facilitate nothing.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 83
    temperature_celsius: 26
    replicates: 2
  - step_description: Cells were incubated with trypsin-edta to facilitate control.
    conditions_or_variables:
    - with protease inhibitors
    - adherent culture
    data_collected: true
    duration_minutes: 641
    temperature_celsius: 30
    replicates: 4
control_groups:
- control_type: Vehicle Control
  description: Task most add trial similar whether itself food ability religious build
    record three clearly.
data_analysis_methods:
- Statistical analysis using GraphPad Prism (unpaired t-tests)
- ImageJ densitometry
