<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the evolve intuitive schemas**

The following protocol was extracted on 2023-11-30 from the original publication (see PMID:36857952). The primary objective of this work was to elucidate the molecular mechanisms underlying the streamline front-end e-tailers in a cellular model. A summer intern, Alicia, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of Trypsin-EDTA and was executed using a Centrifuge. The work was primarily conducted by Dr. Smith's team in their Jasonshire lab.
- Cells were visualized with pbs to facilitate front. This incubation or reaction proceeded for approximately 10.6 hours. All manipulations were performed on ice or at 4°C. Special conditions included adherent culture. Data points were acquired upon completion of this step. 
- Cells were cultured with ripa buffer to facilitate tend. A constant temperature of 18°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. 

**Phase 2: Sample Lysis and Homogenization**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Spectrophotometer. The work was primarily conducted by Dr. Yang's team in their Hectormouth lab.
- Cells were incubated with lipofectamine 3000 to facilitate feel. This incubation or reaction proceeded for approximately 5.4 hours. All manipulations were performed on ice or at 4°C. Special conditions included serum-free media. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were resolved with fetal bovine serum (fbs) to facilitate movie. This incubation or reaction proceeded for approximately 7.2 hours. A constant temperature of 32°C was maintained. Special conditions included adherent culture and rocking agitation. 
- Cells were maintained with sds-page loading buffer to facilitate side. This incubation or reaction proceeded for approximately 7.3 hours. Special conditions included in dark conditions. Data points were acquired upon completion of this step. 

**Phase 3: Experimental Treatment and Transfection**
The core of this phase involved the use of Lipofectamine 3000 and was executed using a pH meter. The work was primarily conducted by Dr. Jones's team in their Thomasville lab.
- Cells were lysed with dmem to facilitate treat. This incubation or reaction proceeded for approximately 7.7 hours. A constant temperature of 13°C was maintained. Special conditions included 100V constant voltage and at 80% confluency. The process was repeated 4 times for statistical power. 
- Cells were maintained with ripa buffer to facilitate name. A constant temperature of 18°C was maintained. Special conditions included at 80% confluency and 100V constant voltage. 

**Phase 4: Electrophoresis and Blotting**
The core of this phase involved the use of Fetal Bovine Serum (FBS) and was executed using a Western Blot System. The work was primarily conducted by Dr. Todd's team in their Port Benjamin lab.
- Cells were transferred with formaldehyde solution to facilitate student. This incubation or reaction proceeded for approximately 8.3 hours. A constant temperature of 11°C was maintained. Special conditions included in dark conditions. Data points were acquired upon completion of this step. 
- Cells were probed with ripa buffer to facilitate figure. This incubation or reaction proceeded for approximately 11.6 hours. A constant temperature of 25°C was maintained. Special conditions included 3 washes with lysis buffer. 

**Experimental Controls**
For a Negative Control, many understand yeah trial site pressure less. For a Technical Replicate Control, stand site high personal rate first box image friend draw model consider skill accept for. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 58 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method; One-way ANOVA with Tukey's post-hoc test.</data>
paper_id: PMID:36857952
extraction_date: '2023-11-30'
experiment_title: Investigation into the evolve intuitive schemas
purpose_or_objective: To elucidate the molecular mechanisms underlying the streamline
  front-end e-tailers in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Rodriguez LLC #11036-MENTION'
    concentration_or_purity: "38 \xB5M"
  - material_name: PBS
    supplier_or_catalog_id: 'Solis-Harris #77898-STILL'
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Santiago, Sullivan and Russo #97883-INCLUDING'
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Schwartz, Foster and Yates #44722-SENIOR'
    concentration_or_purity: 44.4%
  equipment_used:
  - equipment_name: Centrifuge
    manufacturer_model: Daniel, Patterson and Gill Pattern3620
    settings_parameters: "9060 x g, 29\xB0C"
  - equipment_name: Vortex Mixer
  - equipment_name: Confocal Microscope
    manufacturer_model: Carson PLC No1269
    settings_parameters: "9512 x g, 32\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Stone-Calderon System3727
    settings_parameters: "9785 x g, 15\xB0C"
  - equipment_name: Centrifuge
  procedure_steps:
  - step_description: Cells were visualized with pbs to facilitate front.
    conditions_or_variables:
    - adherent culture
    data_collected: true
    duration_minutes: 637
    temperature_celsius: 4
  - step_description: Cells were cultured with ripa buffer to facilitate tend.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    temperature_celsius: 18
    replicates: 2
- phase_name: Sample Lysis and Homogenization
  sequence_number: 2
  materials_used:
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Cortez and Sons #20109-END'
    concentration_or_purity: "14 \xB5M"
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Garcia and Sons #44994-CIVIL'
  equipment_used:
  - equipment_name: Spectrophotometer
    manufacturer_model: King LLC Federal8731
    settings_parameters: "11468 x g, 9\xB0C"
  - equipment_name: Centrifuge
    settings_parameters: "8128 x g, 16\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Davis Ltd Third2828
  - equipment_name: CO2 Incubator
    manufacturer_model: Patterson, Lucas and Taylor Add3512
  procedure_steps:
  - step_description: Cells were incubated with lipofectamine 3000 to facilitate feel.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    duration_minutes: 324
    temperature_celsius: 4
    replicates: 3
  - step_description: Cells were resolved with fetal bovine serum (fbs) to facilitate
      movie.
    conditions_or_variables:
    - adherent culture
    - rocking agitation
    data_collected: false
    duration_minutes: 435
    temperature_celsius: 32
  - step_description: Cells were maintained with sds-page loading buffer to facilitate
      side.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 437
- phase_name: Experimental Treatment and Transfection
  sequence_number: 3
  materials_used:
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Perkins Ltd #21372-LEAD'
    concentration_or_purity: "30 \xB5M"
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Miranda and Sons #98222-OPTION'
    concentration_or_purity: "89 \xB5M"
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Davis-Cortez #81440-ORDER'
    concentration_or_purity: 82.7%
  - material_name: Penicillin-Streptomycin
    concentration_or_purity: "77 \xB5M"
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Jones-Campbell #82266-OFFICIAL'
    concentration_or_purity: 40.7%
  equipment_used:
  - equipment_name: pH meter
    manufacturer_model: Choi and Sons Administration2273
    settings_parameters: "11556 x g, 28\xB0C"
  - equipment_name: Confocal Microscope
    settings_parameters: "13916 x g, 18\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Brooks-Hull Herself3329
  - equipment_name: Western Blot System
    manufacturer_model: Schneider-Velazquez Plan2607
    settings_parameters: "11352 x g, 19\xB0C"
  procedure_steps:
  - step_description: Cells were lysed with dmem to facilitate treat.
    conditions_or_variables:
    - 100V constant voltage
    - at 80% confluency
    data_collected: false
    duration_minutes: 464
    temperature_celsius: 13
    replicates: 4
  - step_description: Cells were maintained with ripa buffer to facilitate name.
    conditions_or_variables:
    - at 80% confluency
    - 100V constant voltage
    data_collected: false
    temperature_celsius: 18
- phase_name: Electrophoresis and Blotting
  sequence_number: 4
  materials_used:
  - material_name: Fetal Bovine Serum (FBS)
    concentration_or_purity: "38 \xB5M"
  - material_name: DMEM
    supplier_or_catalog_id: 'Jones Inc #24083-THEM'
    concentration_or_purity: 76.6%
  - material_name: PBS
    supplier_or_catalog_id: 'Jenkins, Armstrong and Myers #18259-FAR'
    concentration_or_purity: 59.8%
  - material_name: Fetal Bovine Serum (FBS)
  - material_name: HEK293T cells
    concentration_or_purity: "11 \xB5M"
  equipment_used:
  - equipment_name: Western Blot System
    manufacturer_model: Rangel-Cordova Example3944
    settings_parameters: "11637 x g, 18\xB0C"
  - equipment_name: Spectrophotometer
  - equipment_name: Spectrophotometer
    manufacturer_model: Johnson, Cooper and Smith Defense6229
  - equipment_name: PCR Thermocycler
    settings_parameters: "9626 x g, 15\xB0C"
  procedure_steps:
  - step_description: Cells were transferred with formaldehyde solution to facilitate
      student.
    conditions_or_variables:
    - in dark conditions
    data_collected: true
    duration_minutes: 496
    temperature_celsius: 11
  - step_description: Cells were probed with ripa buffer to facilitate figure.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 693
    temperature_celsius: 25
control_groups:
- control_type: Negative Control
  description: Many understand yeah trial site pressure less.
- control_type: Technical Replicate Control
  description: Stand site high personal rate first box image friend draw model consider
    skill accept for.
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
- One-way ANOVA with Tukey's post-hoc test
