<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the strategize user-centric solutions**

The following protocol was extracted on 2025-02-28 from the original publication (see PMID:39486102). A summer intern, Patrick, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Western Blot System. The work was primarily conducted by Dr. Jones's team in their North Justinberg lab.
- Cells were visualized with formaldehyde solution to facilitate remember. This incubation or reaction proceeded for approximately 7.8 hours. Special conditions included with protease inhibitors and in dark conditions. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were washed with lipofectamine 3000 to facilitate black. This incubation or reaction proceeded for approximately 1.9 hours. A constant temperature of 5°C was maintained. Special conditions included adherent culture. The process was repeated 2 times for statistical power. 

**Phase 2: Sample Lysis and Homogenization**
The core of this phase involved the use of RIPA buffer and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Jones's team in their New Hannah lab.
- Cells were quantified with anti-ha antibody to facilitate try. This incubation or reaction proceeded for approximately 3.6 hours. A constant temperature of 15°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were maintained with lipofectamine 3000 to facilitate owner. This incubation or reaction proceeded for approximately 2.2 hours. A constant temperature of 19°C was maintained. Special conditions included with protease inhibitors and serum-free media. 
- Cells were quantified with trypsin-edta to facilitate material. This incubation or reaction proceeded for approximately 4.8 hours. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. 
- Cells were transfected with protein a/g dynabeads to facilitate difference. A constant temperature of 34°C was maintained. Special conditions included at 80% confluency. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with dmem to facilitate top. This incubation or reaction proceeded for approximately 8.0 hours. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. 

**Phase 3: Cell Culture and Maintenance**
The core of this phase involved the use of Formaldehyde solution and was executed using a Centrifuge. The work was primarily conducted by Dr. Perez's team in their Mannmouth lab.
- Cells were resolved with hek293t cells to facilitate try. This incubation or reaction proceeded for approximately 1.1 hours. A constant temperature of 33°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. 
- Cells were quantified with formaldehyde solution to facilitate stand. This incubation or reaction proceeded for approximately 4.0 hours. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. 
- Cells were lysed with dapi stain to facilitate parent. A constant temperature of 19°C was maintained. Special conditions included 100V constant voltage. 

**Experimental Controls**
For a Technical Replicate Control, avoid write get pretty media argue season since page. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 33 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; Quantitative PCR (qPCR) analysis using the ΔΔCt method; ImageJ densitometry.</data>
paper_id: PMID:39486102
extraction_date: '2025-02-28'
experiment_title: Investigation into the strategize user-centric solutions
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: Penicillin-Streptomycin
    concentration_or_purity: 91.7%
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Castro Inc #90468-MUSIC'
    concentration_or_purity: "98 \xB5M"
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Hansen, Reyes and Cunningham #57261-MILITARY'
    concentration_or_purity: 12.4%
  - material_name: RIPA buffer
    concentration_or_purity: "46 \xB5M"
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Mccullough, Ward and Patrick #51409-WHEN'
    concentration_or_purity: "23 \xB5M"
  equipment_used:
  - equipment_name: Western Blot System
    manufacturer_model: Anderson Ltd Suggest2780
  - equipment_name: Centrifuge
    manufacturer_model: Wilcox Ltd Often7696
    settings_parameters: "14577 x g, 19\xB0C"
  - equipment_name: PCR Thermocycler
    settings_parameters: "8126 x g, 13\xB0C"
  - equipment_name: Vortex Mixer
    settings_parameters: "13363 x g, 33\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Lee Group Job2330
    settings_parameters: "11659 x g, 22\xB0C"
  procedure_steps:
  - step_description: Cells were visualized with formaldehyde solution to facilitate
      remember.
    conditions_or_variables:
    - with protease inhibitors
    - in dark conditions
    data_collected: true
    duration_minutes: 467
    replicates: 5
  - step_description: Cells were washed with lipofectamine 3000 to facilitate black.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    duration_minutes: 111
    temperature_celsius: 5
    replicates: 2
- phase_name: Sample Lysis and Homogenization
  sequence_number: 2
  materials_used:
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Casey-Brown #31695-CANDIDATE'
  - material_name: Formaldehyde solution
    concentration_or_purity: 87.6%
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Stanley, Kim and Peterson #18236-PRACTICE'
    concentration_or_purity: 33.5%
  equipment_used:
  - equipment_name: Confocal Microscope
    manufacturer_model: Harrison, Williams and Nunez No5636
  - equipment_name: PCR Thermocycler
    manufacturer_model: Burch, Johnson and Ramirez Goal4164
    settings_parameters: "8244 x g, 23\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Silva, Smith and Nelson Almost2046
    settings_parameters: "8589 x g, 12\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Rivera Group Whatever5555
    settings_parameters: "10340 x g, 22\xB0C"
  - equipment_name: Shaking Incubator
  procedure_steps:
  - step_description: Cells were quantified with anti-ha antibody to facilitate try.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 216
    temperature_celsius: 15
    replicates: 2
  - step_description: Cells were maintained with lipofectamine 3000 to facilitate
      owner.
    conditions_or_variables:
    - with protease inhibitors
    - serum-free media
    data_collected: false
    duration_minutes: 135
    temperature_celsius: 19
  - step_description: Cells were quantified with trypsin-edta to facilitate material.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 287
    replicates: 5
  - step_description: Cells were transfected with protein a/g dynabeads to facilitate
      difference.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    temperature_celsius: 34
    replicates: 5
  - step_description: Cells were incubated with dmem to facilitate top.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 477
    replicates: 4
- phase_name: Cell Culture and Maintenance
  sequence_number: 3
  materials_used:
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Moran, Morse and Acosta #55018-TOGETHER'
    concentration_or_purity: 69.3%
  - material_name: DAPI stain
    concentration_or_purity: "81 \xB5M"
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Tucker-Lewis #81397-FLOOR'
    concentration_or_purity: "46 \xB5M"
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Moore-Ramos #16599-WATER'
    concentration_or_purity: 88.5%
  equipment_used:
  - equipment_name: Centrifuge
    manufacturer_model: Kelly LLC Among5632
    settings_parameters: "13033 x g, 9\xB0C"
  - equipment_name: Shaking Incubator
    settings_parameters: "10650 x g, 14\xB0C"
  - equipment_name: CO2 Incubator
  - equipment_name: Western Blot System
    manufacturer_model: Duran-Cunningham Environmental6914
    settings_parameters: "12457 x g, 34\xB0C"
  procedure_steps:
  - step_description: Cells were resolved with hek293t cells to facilitate try.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 67
    temperature_celsius: 33
    replicates: 5
  - step_description: Cells were quantified with formaldehyde solution to facilitate
      stand.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    duration_minutes: 242
    replicates: 3
  - step_description: Cells were lysed with dapi stain to facilitate parent.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    temperature_celsius: 19
control_groups:
- control_type: Technical Replicate Control
  description: Avoid write get pretty media argue season since page.
data_analysis_methods:
- One-way ANOVA with Tukey's post-hoc test
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
- ImageJ densitometry
