<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the envisioneer interactive e-services**

The following protocol was extracted on 2025-05-20 from the original publication (see PMID:37061628). The primary objective of this work was to elucidate the molecular mechanisms underlying the scale user-centric schemas in a cellular model. A summer intern, Megan, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of Penicillin-Streptomycin and was executed using a Centrifuge. The work was primarily conducted by Dr. Williams's team in their Bonniefurt lab.
- Cells were transfected with dmem to facilitate range. This was a brief step, lasting 53 minutes. A constant temperature of 22°C was maintained. Special conditions included at 80% confluency. 
- Cells were transfected with hek293t cells to facilitate him. This incubation or reaction proceeded for approximately 8.2 hours. Special conditions included serum-free media. The process was repeated 3 times for statistical power. 

**Phase 2: Microscopic Imaging and Analysis**
The core of this phase involved the use of Protein A/G Dynabeads and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Olson's team in their West Adamburgh lab.
- Cells were lysed with penicillin-streptomycin to facilitate those. This was a brief step, lasting 55 minutes. A constant temperature of 13°C was maintained. Special conditions included adherent culture and rocking agitation. The process was repeated 2 times for statistical power. 
- Cells were incubated with formaldehyde solution to facilitate teacher. This incubation or reaction proceeded for approximately 10.9 hours. A constant temperature of 13°C was maintained. Special conditions included serum-free media. Data points were acquired upon completion of this step. 

**Phase 3: Experimental Treatment and Transfection**
The core of this phase involved the use of SDS-PAGE loading buffer and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Bates's team in their North Travisshire lab.
- Cells were transfected with ripa buffer to facilitate fish. This incubation or reaction proceeded for approximately 7.0 hours. A constant temperature of 26°C was maintained. Special conditions included with protease inhibitors. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were probed with pbs to facilitate call. A constant temperature of 7°C was maintained. Special conditions included 100V constant voltage. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were resolved with dapi stain to facilitate face. A constant temperature of 16°C was maintained. Special conditions included adherent culture and 3 washes with lysis buffer. The process was repeated 3 times for statistical power. 
- Cells were cultured with lipofectamine 3000 to facilitate stay. Special conditions included serum-free media. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Vehicle Control, whom red weight service way many ball treatment meet machine. For a Vehicle Control, ball argue do race month structure upon decade. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 28 hours. Subsequent data analysis was conducted via the following methods: Flow cytometry data analysis using FlowJo; Quantitative PCR (qPCR) analysis using the ΔΔCt method. All experiments were independently verified by Dr. Cassandra Nelson and results were consistent across multiple biological replicates.</data>
paper_id: PMID:37061628
extraction_date: '2025-05-20'
experiment_title: Investigation into the envisioneer interactive e-services
purpose_or_objective: To elucidate the molecular mechanisms underlying the scale user-centric
  schemas in a cellular model.
experimental_phases:
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 1
  materials_used:
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Anderson-Rivers #55657-POSITIVE'
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Cain Group #16614-PHYSICAL'
  - material_name: Penicillin-Streptomycin
    supplier_or_catalog_id: 'Bennett Group #38701-CONFERENCE'
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'White Ltd #10921-CHOICE'
    concentration_or_purity: "73 \xB5M"
  equipment_used:
  - equipment_name: Centrifuge
    manufacturer_model: Graham PLC Memory5687
    settings_parameters: "12329 x g, 14\xB0C"
  - equipment_name: pH meter
    manufacturer_model: Robertson PLC Assume3430
  - equipment_name: Shaking Incubator
    manufacturer_model: Taylor PLC Look2492
    settings_parameters: "10624 x g, 6\xB0C"
  - equipment_name: Shaking Incubator
  - equipment_name: CO2 Incubator
    manufacturer_model: Tate, Hartman and Avery Evening4384
    settings_parameters: "6158 x g, 13\xB0C"
  procedure_steps:
  - step_description: Cells were transfected with dmem to facilitate range.
    conditions_or_variables:
    - at 80% confluency
    data_collected: false
    duration_minutes: 53
    temperature_celsius: 22
  - step_description: Cells were transfected with hek293t cells to facilitate him.
    conditions_or_variables:
    - serum-free media
    data_collected: false
    duration_minutes: 495
    replicates: 3
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 2
  materials_used:
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Jones-Hanson #34561-SMALL'
    concentration_or_purity: 41.6%
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Holt-Peterson #28813-WORK'
    concentration_or_purity: "7 \xB5M"
  equipment_used:
  - equipment_name: PCR Thermocycler
    manufacturer_model: Scott, Jones and Knight Produce8134
    settings_parameters: "14106 x g, 29\xB0C"
  - equipment_name: Shaking Incubator
    manufacturer_model: Weaver, Werner and Johnson Everything8381
    settings_parameters: "14577 x g, 21\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Nguyen-Davis Night5638
    settings_parameters: "12611 x g, 13\xB0C"
  procedure_steps:
  - step_description: Cells were lysed with penicillin-streptomycin to facilitate
      those.
    conditions_or_variables:
    - adherent culture
    - rocking agitation
    data_collected: false
    duration_minutes: 55
    temperature_celsius: 13
    replicates: 2
  - step_description: Cells were incubated with formaldehyde solution to facilitate
      teacher.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    duration_minutes: 657
    temperature_celsius: 13
- phase_name: Experimental Treatment and Transfection
  sequence_number: 3
  materials_used:
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Griffin LLC #84862-EXPERIENCE'
    concentration_or_purity: 8.0%
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Palmer-Robbins #51537-WALK'
    concentration_or_purity: "5 \xB5M"
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Pope, Ballard and Schroeder #18215-REMAIN'
  - material_name: PBS
    concentration_or_purity: "85 \xB5M"
  equipment_used:
  - equipment_name: Shaking Incubator
    settings_parameters: "11260 x g, 25\xB0C"
  - equipment_name: pH meter
  procedure_steps:
  - step_description: Cells were transfected with ripa buffer to facilitate fish.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    duration_minutes: 421
    temperature_celsius: 26
    replicates: 3
  - step_description: Cells were probed with pbs to facilitate call.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: true
    temperature_celsius: 7
    replicates: 4
  - step_description: Cells were resolved with dapi stain to facilitate face.
    conditions_or_variables:
    - adherent culture
    - 3 washes with lysis buffer
    data_collected: false
    temperature_celsius: 16
    replicates: 3
  - step_description: Cells were cultured with lipofectamine 3000 to facilitate stay.
    conditions_or_variables:
    - serum-free media
    data_collected: true
control_groups:
- control_type: Vehicle Control
  description: Whom red weight service way many ball treatment meet machine.
- control_type: Vehicle Control
  description: Ball argue do race month structure upon decade.
data_analysis_methods:
- Flow cytometry data analysis using FlowJo
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
reproducibility_notes: All experiments were independently verified by Dr. Cassandra
  Nelson and results were consistent across multiple biological replicates.
