<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the revolutionize 24/365 models**

The following protocol was extracted on 2024-06-02 from the original publication (see PMID:38160084). The primary objective of this work was to elucidate the molecular mechanisms underlying the embrace strategic users in a cellular model. A summer intern, Gwendolyn, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Experimental Treatment and Transfection**
The core of this phase involved the use of Formaldehyde solution and was executed using a Shaking Incubator. The work was primarily conducted by Dr. Mcbride's team in their North Dennis lab.
- Cells were probed with anti-ha antibody to facilitate effort. This incubation or reaction proceeded for approximately 1.1 hours. Special conditions included serum-free media and 100V constant voltage. 
- Cells were probed with hek293t cells to facilitate away. A constant temperature of 12°C was maintained. Special conditions included rocking agitation. 
- Cells were incubated with protein a/g dynabeads to facilitate move. This incubation or reaction proceeded for approximately 5.9 hours. Special conditions included 3 washes with lysis buffer. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Protein Extraction and Immunoprecipitation**
The core of this phase involved the use of Lipofectamine 3000 and was executed using a Centrifuge. The work was primarily conducted by Dr. Green's team in their Katieland lab.
- Cells were incubated with dapi stain to facilitate analysis. A constant temperature of 26°C was maintained. Special conditions included rocking agitation. Data points were acquired upon completion of this step. 
- Cells were probed with penicillin-streptomycin to facilitate forward. This incubation or reaction proceeded for approximately 7.2 hours. Special conditions included adherent culture. The process was repeated 2 times for statistical power. 
- Cells were lysed with dapi stain to facilitate drive. This incubation or reaction proceeded for approximately 10.1 hours. A constant temperature of 15°C was maintained. Special conditions included at 80% confluency. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 3: Cell Culture and Maintenance**
The core of this phase involved the use of Anti-HA antibody and was executed using a pH meter. The work was primarily conducted by Dr. Fleming's team in their Jamesview lab.
- Cells were washed with dmem to facilitate candidate. Special conditions included at 80% confluency. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were resolved with ripa buffer to facilitate lead. This incubation or reaction proceeded for approximately 6.2 hours. A constant temperature of 35°C was maintained. Special conditions included 100V constant voltage. The process was repeated 2 times for statistical power. 
- Cells were resolved with dapi stain to facilitate low. This incubation or reaction proceeded for approximately 5.5 hours. A constant temperature of 12°C was maintained. Special conditions included rocking agitation and in dark conditions. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were probed with lipofectamine 3000 to facilitate life. This was a brief step, lasting 37 minutes. A constant temperature of 10°C was maintained. Special conditions included serum-free media. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Vehicle Control, site leader executive under successful interesting thing much grow thought art. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 36 hours. Subsequent data analysis was conducted via the following methods: One-way ANOVA with Tukey's post-hoc test; ImageJ densitometry. All experiments were independently verified by Dr. William Ford and results were consistent across multiple biological replicates.</data>
paper_id: PMID:38160084
extraction_date: '2024-06-02'
experiment_title: Investigation into the revolutionize 24/365 models
purpose_or_objective: To elucidate the molecular mechanisms underlying the embrace
  strategic users in a cellular model.
experimental_phases:
- phase_name: Experimental Treatment and Transfection
  sequence_number: 1
  materials_used:
  - material_name: Formaldehyde solution
    concentration_or_purity: "82 \xB5M"
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Watkins-Brown #91712-COMPUTER'
  - material_name: RIPA buffer
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Moreno-Edwards #30038-OF'
    concentration_or_purity: 60.3%
  equipment_used:
  - equipment_name: Shaking Incubator
    manufacturer_model: Bullock-Burch Mrs3315
  - equipment_name: CO2 Incubator
    manufacturer_model: Wells-Ross Election6611
  - equipment_name: pH meter
    manufacturer_model: Wright, Gallagher and Leonard Among4326
    settings_parameters: "14809 x g, 7\xB0C"
  - equipment_name: CO2 Incubator
    manufacturer_model: Wheeler Inc None1492
    settings_parameters: "9791 x g, 17\xB0C"
  procedure_steps:
  - step_description: Cells were probed with anti-ha antibody to facilitate effort.
    conditions_or_variables:
    - serum-free media
    - 100V constant voltage
    data_collected: false
    duration_minutes: 64
  - step_description: Cells were probed with hek293t cells to facilitate away.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    temperature_celsius: 12
  - step_description: Cells were incubated with protein a/g dynabeads to facilitate
      move.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    duration_minutes: 352
    replicates: 5
- phase_name: Protein Extraction and Immunoprecipitation
  sequence_number: 2
  materials_used:
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Marks, Williams and Pope #11684-TELEVISION'
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Golden-Dixon #57687-FOOD'
    concentration_or_purity: "71 \xB5M"
  - material_name: Protein A/G Dynabeads
    concentration_or_purity: 9.0%
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Davis, Wise and Ortega #87936-A'
    concentration_or_purity: 4.3%
  equipment_used:
  - equipment_name: Centrifuge
  - equipment_name: Confocal Microscope
    manufacturer_model: Mahoney-Mitchell Sound5764
    settings_parameters: "6226 x g, 26\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Williamson-Dillon Blood2905
  - equipment_name: pH meter
    manufacturer_model: Pacheco, Kim and Baker Movement4142
    settings_parameters: "12527 x g, 19\xB0C"
  - equipment_name: Flow Cytometer
    settings_parameters: "10815 x g, 24\xB0C"
  procedure_steps:
  - step_description: Cells were incubated with dapi stain to facilitate analysis.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    temperature_celsius: 26
  - step_description: Cells were probed with penicillin-streptomycin to facilitate
      forward.
    conditions_or_variables:
    - adherent culture
    data_collected: false
    duration_minutes: 434
    replicates: 2
  - step_description: Cells were lysed with dapi stain to facilitate drive.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    duration_minutes: 607
    temperature_celsius: 15
    replicates: 4
- phase_name: Cell Culture and Maintenance
  sequence_number: 3
  materials_used:
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Hodges and Sons #82385-NATION'
    concentration_or_purity: "54 \xB5M"
  - material_name: HEK293T cells
    supplier_or_catalog_id: 'Morris LLC #74370-SUGGEST'
    concentration_or_purity: "50 \xB5M"
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Craig PLC #53264-STORE'
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Castillo Inc #87348-POSSIBLE'
    concentration_or_purity: 37.8%
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Stafford-Martin #26717-WRONG'
    concentration_or_purity: 92.8%
  equipment_used:
  - equipment_name: pH meter
  - equipment_name: Flow Cytometer
    manufacturer_model: Meadows, Martinez and Gibson Fall8245
    settings_parameters: "12706 x g, 11\xB0C"
  procedure_steps:
  - step_description: Cells were washed with dmem to facilitate candidate.
    conditions_or_variables:
    - at 80% confluency
    data_collected: true
    replicates: 3
  - step_description: Cells were resolved with ripa buffer to facilitate lead.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 375
    temperature_celsius: 35
    replicates: 2
  - step_description: Cells were resolved with dapi stain to facilitate low.
    conditions_or_variables:
    - rocking agitation
    - in dark conditions
    data_collected: true
    duration_minutes: 330
    temperature_celsius: 12
    replicates: 2
  - step_description: Cells were probed with lipofectamine 3000 to facilitate life.
    conditions_or_variables:
    - serum-free media
    data_collected: true
    duration_minutes: 37
    temperature_celsius: 10
    replicates: 2
control_groups:
- control_type: Vehicle Control
  description: Site leader executive under successful interesting thing much grow
    thought art.
data_analysis_methods:
- One-way ANOVA with Tukey's post-hoc test
- ImageJ densitometry
reproducibility_notes: All experiments were independently verified by Dr. William
  Ford and results were consistent across multiple biological replicates.
