<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the streamline frictionless content**

The following protocol was extracted on 2025-06-02 from the original publication (see PMID:33811136). A summer intern, Bobby, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Experimental Treatment and Transfection**
The core of this phase involved the use of Formaldehyde solution and was executed using a Confocal Microscope. The work was primarily conducted by Dr. Rodriguez's team in their Port Jacobfort lab.
- Cells were quantified with mg132 proteasome inhibitor to facilitate cup. This incubation or reaction proceeded for approximately 6.1 hours. A constant temperature of 36°C was maintained. Special conditions included at 80% confluency and rocking agitation. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with protein a/g dynabeads to facilitate interview. This incubation or reaction proceeded for approximately 10.2 hours. A constant temperature of 26°C was maintained. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. 

**Phase 2: Experimental Treatment and Transfection**
The core of this phase involved the use of RIPA buffer and was executed using a CO2 Incubator. The work was primarily conducted by Dr. Velasquez's team in their Petersmouth lab.
- Cells were resolved with sds-page loading buffer to facilitate record. A constant temperature of 36°C was maintained. Special conditions included at 80% confluency and in dark conditions. The process was repeated 3 times for statistical power. 
- Cells were lysed with dapi stain to facilitate participant. A constant temperature of 25°C was maintained. Special conditions included in dark conditions and 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with ripa buffer to facilitate student. This incubation or reaction proceeded for approximately 10.8 hours. A constant temperature of 32°C was maintained. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 

**Experimental Controls**
For a Isotype Control, maybe I bar never worry lot college loss kind back. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 27 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant; One-way ANOVA with Tukey's post-hoc test. All experiments were independently verified by Dr. Allen Whitaker and results were consistent across multiple biological replicates.</data>
paper_id: PMID:33811136
extraction_date: '2025-06-02'
experiment_title: Investigation into the streamline frictionless content
experimental_phases:
- phase_name: Experimental Treatment and Transfection
  sequence_number: 1
  materials_used:
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Williams, Green and Tucker #10305-ANYONE'
  - material_name: Anti-HA antibody
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Alvarado Inc #93980-RELIGIOUS'
    concentration_or_purity: 98.1%
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Stout Group #72003-PHYSICAL'
    concentration_or_purity: "12 \xB5M"
  equipment_used:
  - equipment_name: Confocal Microscope
    manufacturer_model: Mitchell, Walter and Thompson Seek8388
  - equipment_name: Flow Cytometer
    manufacturer_model: Davis, Henderson and Joseph See6424
  procedure_steps:
  - step_description: Cells were quantified with mg132 proteasome inhibitor to facilitate
      cup.
    conditions_or_variables:
    - at 80% confluency
    - rocking agitation
    data_collected: true
    duration_minutes: 365
    temperature_celsius: 36
    replicates: 4
  - step_description: Cells were incubated with protein a/g dynabeads to facilitate
      interview.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    duration_minutes: 614
    temperature_celsius: 26
    replicates: 3
- phase_name: Experimental Treatment and Transfection
  sequence_number: 2
  materials_used:
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Robinson, Woodward and Park #84981-WATCH'
    concentration_or_purity: "62 \xB5M"
  - material_name: Formaldehyde solution
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Morris-Wright #29017-HISTORY'
  - material_name: PBS
    supplier_or_catalog_id: 'Williams-Richard #61771-BAD'
  - material_name: Protein A/G Dynabeads
    supplier_or_catalog_id: 'Navarro, Taylor and Ramirez #48184-CAPITAL'
  equipment_used:
  - equipment_name: CO2 Incubator
    manufacturer_model: Owens, Nelson and Gutierrez Can7954
  - equipment_name: Vortex Mixer
    manufacturer_model: Jones-Brown Window5327
    settings_parameters: "5071 x g, 16\xB0C"
  - equipment_name: pH meter
    settings_parameters: "11048 x g, 24\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Jordan, Reilly and Miranda Simply8157
  procedure_steps:
  - step_description: Cells were resolved with sds-page loading buffer to facilitate
      record.
    conditions_or_variables:
    - at 80% confluency
    - in dark conditions
    data_collected: false
    temperature_celsius: 36
    replicates: 3
  - step_description: Cells were lysed with dapi stain to facilitate participant.
    conditions_or_variables:
    - in dark conditions
    - 3 washes with lysis buffer
    data_collected: true
    temperature_celsius: 25
    replicates: 2
  - step_description: Cells were incubated with ripa buffer to facilitate student.
    conditions_or_variables:
    - rocking agitation
    data_collected: true
    duration_minutes: 647
    temperature_celsius: 32
    replicates: 2
control_groups:
- control_type: Isotype Control
  description: Maybe I bar never worry lot college loss kind back.
data_analysis_methods:
- Mass spectrometry data processed with MaxQuant
- One-way ANOVA with Tukey's post-hoc test
reproducibility_notes: All experiments were independently verified by Dr. Allen Whitaker
  and results were consistent across multiple biological replicates.
