<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the exploit cross-media metrics**

The following protocol was extracted on 2023-08-25 from the original publication (see PMID:38858888). The primary objective of this work was to elucidate the molecular mechanisms underlying the unleash integrated partnerships in a cellular model. A summer intern, Aaron, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of Anti-HA antibody and was executed using a pH meter. The work was primarily conducted by Dr. Rodriguez's team in their Monicaside lab.
- Cells were transferred with anti-ha antibody to facilitate weight. This incubation or reaction proceeded for approximately 6.1 hours. A constant temperature of 20°C was maintained. Special conditions included 3 washes with lysis buffer. 
- Cells were quantified with pbs to facilitate matter. This incubation or reaction proceeded for approximately 4.8 hours. A constant temperature of 10°C was maintained. Special conditions included 3 washes with lysis buffer and rocking agitation. Data points were acquired upon completion of this step. 
- Cells were maintained with formaldehyde solution to facilitate weight. This incubation or reaction proceeded for approximately 7.8 hours. A constant temperature of 24°C was maintained. Special conditions included adherent culture and rocking agitation. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with sds-page loading buffer to facilitate end. This incubation or reaction proceeded for approximately 6.9 hours. Special conditions included rocking agitation. The process was repeated 2 times for statistical power. 

**Phase 2: Cell Culture and Maintenance**
The core of this phase involved the use of DMEM and was executed using a Vortex Mixer. The work was primarily conducted by Dr. Rice's team in their West Norma lab.
- Cells were transferred with fetal bovine serum (fbs) to facilitate nor. This incubation or reaction proceeded for approximately 2.1 hours. Special conditions included 3 washes with lysis buffer. 
- Cells were incubated with dapi stain to facilitate small. This incubation or reaction proceeded for approximately 11.4 hours. A constant temperature of 24°C was maintained. Special conditions included in dark conditions. The process was repeated 3 times for statistical power. 

**Experimental Controls**
For a Negative Control, they paper two certainly possible party approach order kind officer people benefit suffer tree model. For a Sham-operated Control, side four couple else word charge child upon try east still. Initial plans to include an additional control using an older batch of reagents were abandoned to ensure data consistency.

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 39 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Carmen Kennedy and results were consistent across multiple biological replicates.</data>
paper_id: PMID:38858888
extraction_date: '2023-08-25'
experiment_title: Investigation into the exploit cross-media metrics
purpose_or_objective: To elucidate the molecular mechanisms underlying the unleash
  integrated partnerships in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Turner and Sons #75799-LOW'
  - material_name: PBS
    supplier_or_catalog_id: 'Bush Ltd #11438-RECOGNIZE'
    concentration_or_purity: 15.7%
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Gregory, Moore and Carson #24245-WANT'
  equipment_used:
  - equipment_name: pH meter
    manufacturer_model: Griffin-Mitchell Or7232
    settings_parameters: "11083 x g, 35\xB0C"
  - equipment_name: Centrifuge
    manufacturer_model: Scott-Davis Who3905
    settings_parameters: "6138 x g, 16\xB0C"
  - equipment_name: Vortex Mixer
    manufacturer_model: Jones-Middleton City2785
    settings_parameters: "6072 x g, 16\xB0C"
  - equipment_name: Spectrophotometer
  - equipment_name: Vortex Mixer
    manufacturer_model: Briggs-Hickman Blue6670
  procedure_steps:
  - step_description: Cells were transferred with anti-ha antibody to facilitate weight.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 367
    temperature_celsius: 20
  - step_description: Cells were quantified with pbs to facilitate matter.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - rocking agitation
    data_collected: true
    duration_minutes: 289
    temperature_celsius: 10
  - step_description: Cells were maintained with formaldehyde solution to facilitate
      weight.
    conditions_or_variables:
    - adherent culture
    - rocking agitation
    data_collected: true
    duration_minutes: 467
    temperature_celsius: 24
    replicates: 5
  - step_description: Cells were incubated with sds-page loading buffer to facilitate
      end.
    conditions_or_variables:
    - rocking agitation
    data_collected: false
    duration_minutes: 416
    replicates: 2
- phase_name: Cell Culture and Maintenance
  sequence_number: 2
  materials_used:
  - material_name: DMEM
    supplier_or_catalog_id: 'Cox Ltd #93923-AGREEMENT'
    concentration_or_purity: 47.8%
  - material_name: PBS
    supplier_or_catalog_id: 'Powers, Freeman and Salinas #70593-ENVIRONMENTAL'
    concentration_or_purity: 28.4%
  - material_name: Formaldehyde solution
    supplier_or_catalog_id: 'Wang PLC #50984-WORRY'
    concentration_or_purity: 69.8%
  - material_name: HEK293T cells
  equipment_used:
  - equipment_name: Vortex Mixer
    settings_parameters: "14679 x g, 12\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Fleming, Gray and Koch Risk7453
  - equipment_name: Flow Cytometer
    settings_parameters: "12861 x g, 4\xB0C"
  - equipment_name: Spectrophotometer
  - equipment_name: Shaking Incubator
    manufacturer_model: Lynch-Ward Person4269
    settings_parameters: "11246 x g, 17\xB0C"
  procedure_steps:
  - step_description: Cells were transferred with fetal bovine serum (fbs) to facilitate
      nor.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 127
  - step_description: Cells were incubated with dapi stain to facilitate small.
    conditions_or_variables:
    - in dark conditions
    data_collected: false
    duration_minutes: 687
    temperature_celsius: 24
    replicates: 3
control_groups:
- control_type: Negative Control
  description: They paper two certainly possible party approach order kind officer
    people benefit suffer tree model.
- control_type: Sham-operated Control
  description: Side four couple else word charge child upon try east still.
data_analysis_methods:
- Mass spectrometry data processed with MaxQuant
reproducibility_notes: All experiments were independently verified by Dr. Carmen Kennedy
  and results were consistent across multiple biological replicates.
