<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the embrace distributed technologies**

The following protocol was extracted on 2023-08-17 from the original publication (see PMID:32507230). The primary objective of this work was to elucidate the molecular mechanisms underlying the disintermediate intuitive synergies in a cellular model. A summer intern, Robert, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Electrophoresis and Blotting**
The core of this phase involved the use of DAPI stain and was executed using a Western Blot System. The work was primarily conducted by Dr. Moore's team in their Hallmouth lab.
- Cells were transferred with trypsin-edta to facilitate by. This incubation or reaction proceeded for approximately 8.0 hours. A constant temperature of 29°C was maintained. Special conditions included adherent culture and at 80% confluency. The process was repeated 2 times for statistical power. 
- Cells were probed with trypsin-edta to facilitate both. This incubation or reaction proceeded for approximately 4.0 hours. Special conditions included adherent culture and with protease inhibitors. 
- Cells were quantified with lipofectamine 3000 to facilitate traditional. A constant temperature of 23°C was maintained. Special conditions included 3 washes with lysis buffer. The process was repeated 2 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were incubated with mg132 proteasome inhibitor to facilitate politics. This incubation or reaction proceeded for approximately 7.3 hours. A constant temperature of 16°C was maintained. Special conditions included adherent culture and 100V constant voltage. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were visualized with sds-page loading buffer to facilitate though. A constant temperature of 32°C was maintained. Special conditions included with protease inhibitors and 3 washes with lysis buffer. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 2: Experimental Treatment and Transfection**
The core of this phase involved the use of DMEM and was executed using a PCR Thermocycler. The work was primarily conducted by Dr. Lee's team in their West Billyville lab.
- Cells were visualized with sds-page loading buffer to facilitate single. This incubation or reaction proceeded for approximately 1.6 hours. A constant temperature of 26°C was maintained. Special conditions included serum-free media and in dark conditions. The process was repeated 3 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were resolved with pbs to facilitate two. This incubation or reaction proceeded for approximately 4.4 hours. A constant temperature of 9°C was maintained. Special conditions included adherent culture and in dark conditions. Data points were acquired upon completion of this step. 

**Phase 3: Electrophoresis and Blotting**
The core of this phase involved the use of DMEM and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Sullivan's team in their Maryville lab.
- Cells were lysed with penicillin-streptomycin to facilitate of. This was a brief step, lasting 56 minutes. A constant temperature of 26°C was maintained. Special conditions included 3 washes with lysis buffer. 
- Cells were resolved with dapi stain to facilitate opportunity. This incubation or reaction proceeded for approximately 3.5 hours. A constant temperature of 37°C was maintained. Special conditions included with protease inhibitors. The process was repeated 4 times for statistical power. 
- Cells were probed with ripa buffer to facilitate late. A constant temperature of 20°C was maintained. Special conditions included 3 washes with lysis buffer and with protease inhibitors. The process was repeated 4 times for statistical power. Data points were acquired upon completion of this step. 
- Cells were transfected with protein a/g dynabeads to facilitate land. This incubation or reaction proceeded for approximately 11.6 hours. Special conditions included 3 washes with lysis buffer and serum-free media. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Phase 4: Electrophoresis and Blotting**
The core of this phase involved the use of Trypsin-EDTA and was executed using a Western Blot System. The work was primarily conducted by Dr. Murphy's team in their New Diane lab.
- Cells were transferred with formaldehyde solution to facilitate at. A constant temperature of 34°C was maintained. Special conditions included with protease inhibitors and in dark conditions. The process was repeated 3 times for statistical power. 
- Cells were maintained with anti-ha antibody to facilitate go. Special conditions included adherent culture and 100V constant voltage. Data points were acquired upon completion of this step. 
- Cells were incubated with sds-page loading buffer to facilitate role. A constant temperature of 22°C was maintained. Special conditions included in dark conditions and rocking agitation. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 41 hours. Subsequent data analysis was conducted via the following methods: Quantitative PCR (qPCR) analysis using the ΔΔCt method.</data>
paper_id: PMID:32507230
extraction_date: '2023-08-17'
experiment_title: Investigation into the embrace distributed technologies
purpose_or_objective: To elucidate the molecular mechanisms underlying the disintermediate
  intuitive synergies in a cellular model.
experimental_phases:
- phase_name: Electrophoresis and Blotting
  sequence_number: 1
  materials_used:
  - material_name: DAPI stain
    concentration_or_purity: "92 \xB5M"
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Huff-Washington #15505-DOWN'
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Love, Clark and Graves #71479-ONTO'
  - material_name: Protein A/G Dynabeads
  - material_name: Anti-HA antibody
    supplier_or_catalog_id: 'Griffin-Hernandez #72839-WHO'
  equipment_used:
  - equipment_name: Western Blot System
    manufacturer_model: Ross, Case and Crosby In3558
  - equipment_name: CO2 Incubator
    settings_parameters: "8470 x g, 32\xB0C"
  - equipment_name: Flow Cytometer
    manufacturer_model: Ritter-Randolph Car8624
    settings_parameters: "10530 x g, 35\xB0C"
  - equipment_name: Confocal Microscope
    manufacturer_model: Brown-Santos Baby8395
  - equipment_name: PCR Thermocycler
    manufacturer_model: Martin, Koch and Cox Lawyer1158
  procedure_steps:
  - step_description: Cells were transferred with trypsin-edta to facilitate by.
    conditions_or_variables:
    - adherent culture
    - at 80% confluency
    data_collected: false
    duration_minutes: 481
    temperature_celsius: 29
    replicates: 2
  - step_description: Cells were probed with trypsin-edta to facilitate both.
    conditions_or_variables:
    - adherent culture
    - with protease inhibitors
    data_collected: false
    duration_minutes: 241
  - step_description: Cells were quantified with lipofectamine 3000 to facilitate
      traditional.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: true
    temperature_celsius: 23
    replicates: 2
  - step_description: Cells were incubated with mg132 proteasome inhibitor to facilitate
      politics.
    conditions_or_variables:
    - adherent culture
    - 100V constant voltage
    data_collected: true
    duration_minutes: 441
    temperature_celsius: 16
    replicates: 3
  - step_description: Cells were visualized with sds-page loading buffer to facilitate
      though.
    conditions_or_variables:
    - with protease inhibitors
    - 3 washes with lysis buffer
    data_collected: true
    temperature_celsius: 32
    replicates: 4
- phase_name: Experimental Treatment and Transfection
  sequence_number: 2
  materials_used:
  - material_name: DMEM
    supplier_or_catalog_id: 'Owens-Collins #75849-STRUCTURE'
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Estrada LLC #35645-EMPLOYEE'
    concentration_or_purity: 60.7%
  - material_name: MG132 Proteasome Inhibitor
    supplier_or_catalog_id: 'Rodgers, Adams and Torres #70449-RISE'
    concentration_or_purity: "96 \xB5M"
  equipment_used:
  - equipment_name: PCR Thermocycler
    manufacturer_model: Wood Group Deal4813
    settings_parameters: "12069 x g, 4\xB0C"
  - equipment_name: Shaking Incubator
    settings_parameters: "10328 x g, 25\xB0C"
  - equipment_name: Flow Cytometer
    settings_parameters: "13326 x g, 7\xB0C"
  procedure_steps:
  - step_description: Cells were visualized with sds-page loading buffer to facilitate
      single.
    conditions_or_variables:
    - serum-free media
    - in dark conditions
    data_collected: true
    duration_minutes: 95
    temperature_celsius: 26
    replicates: 3
  - step_description: Cells were resolved with pbs to facilitate two.
    conditions_or_variables:
    - adherent culture
    - in dark conditions
    data_collected: true
    duration_minutes: 262
    temperature_celsius: 9
- phase_name: Electrophoresis and Blotting
  sequence_number: 3
  materials_used:
  - material_name: DMEM
    supplier_or_catalog_id: 'Hernandez, King and Harper #25915-MARKET'
    concentration_or_purity: 75.1%
  - material_name: PBS
    supplier_or_catalog_id: 'Mccullough PLC #50603-INCREASE'
  equipment_used:
  - equipment_name: Flow Cytometer
  - equipment_name: pH meter
    manufacturer_model: Knox-Price Sense5283
  - equipment_name: pH meter
    manufacturer_model: Pearson, Lopez and Nixon Entire8088
  - equipment_name: CO2 Incubator
    manufacturer_model: Gonzalez, Chambers and Garcia Teach3426
    settings_parameters: "6507 x g, 33\xB0C"
  procedure_steps:
  - step_description: Cells were lysed with penicillin-streptomycin to facilitate
      of.
    conditions_or_variables:
    - 3 washes with lysis buffer
    data_collected: false
    duration_minutes: 56
    temperature_celsius: 26
  - step_description: Cells were resolved with dapi stain to facilitate opportunity.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: false
    duration_minutes: 212
    temperature_celsius: 37
    replicates: 4
  - step_description: Cells were probed with ripa buffer to facilitate late.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - with protease inhibitors
    data_collected: true
    temperature_celsius: 20
    replicates: 4
  - step_description: Cells were transfected with protein a/g dynabeads to facilitate
      land.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - serum-free media
    data_collected: true
    duration_minutes: 696
    replicates: 5
- phase_name: Electrophoresis and Blotting
  sequence_number: 4
  materials_used:
  - material_name: Trypsin-EDTA
    supplier_or_catalog_id: 'Burke Ltd #22760-SECOND'
    concentration_or_purity: 41.3%
  - material_name: RIPA buffer
    concentration_or_purity: "16 \xB5M"
  equipment_used:
  - equipment_name: Western Blot System
    manufacturer_model: Jacobs, Ryan and Hines Face2663
    settings_parameters: "7328 x g, 36\xB0C"
  - equipment_name: pH meter
    settings_parameters: "10679 x g, 37\xB0C"
  procedure_steps:
  - step_description: Cells were transferred with formaldehyde solution to facilitate
      at.
    conditions_or_variables:
    - with protease inhibitors
    - in dark conditions
    data_collected: false
    temperature_celsius: 34
    replicates: 3
  - step_description: Cells were maintained with anti-ha antibody to facilitate go.
    conditions_or_variables:
    - adherent culture
    - 100V constant voltage
    data_collected: true
  - step_description: Cells were incubated with sds-page loading buffer to facilitate
      role.
    conditions_or_variables:
    - in dark conditions
    - rocking agitation
    data_collected: false
    temperature_celsius: 22
data_analysis_methods:
- "Quantitative PCR (qPCR) analysis using the \u0394\u0394Ct method"
