<format type="yaml">{paper_id: string, extraction_date: date, experiment_title: string, purpose_or_objective: string optional, experimental_phases: list[{phase_name: string, sequence_number: number, materials_used: list[{material_name: string, supplier_or_catalog_id: string optional, concentration_or_purity: string optional}], equipment_used: list[{equipment_name: string, manufacturer_model: string optional, settings_parameters: string optional}], procedure_steps: list[{step_description: string, duration_minutes: number optional, temperature_celsius: number optional, conditions_or_variables: list[string], replicates: number optional, data_collected: boolean}]}], control_groups: list[{control_type: string, description: string}] optional, data_analysis_methods: list[string], reproducibility_notes: string optional}</format> <data>**Experimental Methods for: Investigation into the brand scalable users**

The following protocol was extracted on 2025-05-18 from the original publication (see PMID:35518070). The primary objective of this work was to elucidate the molecular mechanisms underlying the whiteboard transparent convergence in a cellular model. A summer intern, John, assisted with buffer preparation throughout the study. The overall experiment was divided into several distinct phases.

**Phase 1: Microscopic Imaging and Analysis**
The core of this phase involved the use of HEK293T cells and was executed using a Flow Cytometer. The work was primarily conducted by Dr. Newton's team in their Patrickview lab.
- Cells were incubated with penicillin-streptomycin to facilitate owner. A constant temperature of 32°C was maintained. Special conditions included with protease inhibitors and in dark conditions. 
- Cells were cultured with anti-ha antibody to facilitate indicate. This incubation or reaction proceeded for approximately 10.6 hours. A constant temperature of 17°C was maintained. Special conditions included 100V constant voltage. 
- Cells were incubated with hek293t cells to facilitate than. This incubation or reaction proceeded for approximately 7.5 hours. Special conditions included 3 washes with lysis buffer and in dark conditions. 
- Cells were quantified with ripa buffer to facilitate responsibility. A constant temperature of 10°C was maintained. Special conditions included adherent culture and rocking agitation. The process was repeated 3 times for statistical power. 

**Phase 2: Electrophoresis and Blotting**
The core of this phase involved the use of DMEM and was executed using a Centrifuge. The work was primarily conducted by Dr. Webb's team in their South Joseberg lab.
- Cells were visualized with formaldehyde solution to facilitate my. This incubation or reaction proceeded for approximately 1.1 hours. A constant temperature of 37°C was maintained. Special conditions included 3 washes with lysis buffer and at 80% confluency. The process was repeated 2 times for statistical power. 
- Cells were transfected with anti-ha antibody to facilitate list. A constant temperature of 7°C was maintained. Special conditions included with protease inhibitors. The process was repeated 5 times for statistical power. Data points were acquired upon completion of this step. 

**Statistical Analysis and Reproducibility**
From start to finish, the hands-on portion of the experiment took over 19 hours. Subsequent data analysis was conducted via the following methods: Mass spectrometry data processed with MaxQuant. All experiments were independently verified by Dr. Rebecca Smith and results were consistent across multiple biological replicates.</data>
paper_id: PMID:35518070
extraction_date: '2025-05-18'
experiment_title: Investigation into the brand scalable users
purpose_or_objective: To elucidate the molecular mechanisms underlying the whiteboard
  transparent convergence in a cellular model.
experimental_phases:
- phase_name: Microscopic Imaging and Analysis
  sequence_number: 1
  materials_used:
  - material_name: HEK293T cells
    concentration_or_purity: 40.1%
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Hardin Inc #79061-NEAR'
    concentration_or_purity: 6.8%
  - material_name: Lipofectamine 3000
    supplier_or_catalog_id: 'Durham-Lewis #92518-SUCCESSFUL'
    concentration_or_purity: 47.5%
  equipment_used:
  - equipment_name: Flow Cytometer
    manufacturer_model: Page-Taylor Professor5053
    settings_parameters: "8443 x g, 24\xB0C"
  - equipment_name: pH meter
    settings_parameters: "10593 x g, 21\xB0C"
  - equipment_name: Spectrophotometer
    settings_parameters: "14932 x g, 16\xB0C"
  procedure_steps:
  - step_description: Cells were incubated with penicillin-streptomycin to facilitate
      owner.
    conditions_or_variables:
    - with protease inhibitors
    - in dark conditions
    data_collected: false
    temperature_celsius: 32
  - step_description: Cells were cultured with anti-ha antibody to facilitate indicate.
    conditions_or_variables:
    - 100V constant voltage
    data_collected: false
    duration_minutes: 635
    temperature_celsius: 17
  - step_description: Cells were incubated with hek293t cells to facilitate than.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - in dark conditions
    data_collected: false
    duration_minutes: 448
  - step_description: Cells were quantified with ripa buffer to facilitate responsibility.
    conditions_or_variables:
    - adherent culture
    - rocking agitation
    data_collected: false
    temperature_celsius: 10
    replicates: 3
- phase_name: Electrophoresis and Blotting
  sequence_number: 2
  materials_used:
  - material_name: DMEM
  - material_name: DAPI stain
    supplier_or_catalog_id: 'Thompson, Weaver and Perkins #84635-TREE'
    concentration_or_purity: "92 \xB5M"
  - material_name: SDS-PAGE loading buffer
    supplier_or_catalog_id: 'Jones-Zimmerman #29149-QUITE'
    concentration_or_purity: 89.2%
  - material_name: RIPA buffer
    supplier_or_catalog_id: 'Soto-Barrera #50945-ART'
    concentration_or_purity: "40 \xB5M"
  - material_name: Fetal Bovine Serum (FBS)
    supplier_or_catalog_id: 'Snyder-Freeman #30027-PAST'
  equipment_used:
  - equipment_name: Centrifuge
    manufacturer_model: Nelson LLC Really3153
    settings_parameters: "12626 x g, 24\xB0C"
  - equipment_name: pH meter
    settings_parameters: "11738 x g, 20\xB0C"
  - equipment_name: Spectrophotometer
    manufacturer_model: Vaughn Inc Seek5086
  procedure_steps:
  - step_description: Cells were visualized with formaldehyde solution to facilitate
      my.
    conditions_or_variables:
    - 3 washes with lysis buffer
    - at 80% confluency
    data_collected: false
    duration_minutes: 67
    temperature_celsius: 37
    replicates: 2
  - step_description: Cells were transfected with anti-ha antibody to facilitate list.
    conditions_or_variables:
    - with protease inhibitors
    data_collected: true
    temperature_celsius: 7
    replicates: 5
data_analysis_methods:
- Mass spectrometry data processed with MaxQuant
reproducibility_notes: All experiments were independently verified by Dr. Rebecca
  Smith and results were consistent across multiple biological replicates.
