ki te whiti i raro nei, me te ka whakahoki i te pātai i muri i roto i te reo ano rite te whiti: Passage: Nucleases he whākōkī e tapahi DNA aho mā te catalyzing te hydrolysis o te hononga phosphodiester. Nucleases e hydrolyse nucleotides i te pito o te aho DNA e huaina exonucleases, ka tapahi endonucleases i roto i aho. Ko te nucleases tino pinepine whakamahia i roto i te koiora rāpoi ko te herenga endonucleases, e tapahi DNA i te raupapa motuhake. Hei tauira, te whākōkī EcoRV whakaaturia ki te maui mohio te raupapa 6-pūtake 5′-GATC-3′, me te hanga i te tapahi i te rārangi whakapae. I roto i te āhua, tiaki enei whākōkī huakita i te huaketo phage mā te tukatuka i te DNA phage ina tomo te pūtau huakita, hei wāhanga o te pūnaha herenga. I roto i te hangarau raupapa, whakamahia enei nucleases- motuhake i roto i te DNA pūkoro cloning me
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