ng phenol and chloroform) or commercial kits can be used for this first step. Next, the DNA sequencing libraries are prepared. This involves fragmenting the DNA, adding sequencing adapters, and DNA amplification if the input DNA is not of sufficient quantity. Recall that for WXS After sequencing, data is analyzed by converting and aligning reads to generate a BAM file. Many analysis tools will use the BAM file to identify variants, which then generates a VCF file. More information about sequencing and BAM a