th. And while shorter sequences are faster to align, they also complicate the layout phase of an assembly as shorter reads are more difficult to use with repeats or near identical repeats.</p>
<p>In the earliest days of DNA sequencing, scientists could only gain a few sequences of short length (some dozen bases) after weeks of work in laboratories. Hence, these sequences could be aligned in a few minutes by hand.</p>
<p>In 1975, the <a href="//en.wikipedia.org/wiki/Dideoxy_termination" title="Dideoxy termin