SKAT</a> method to study CFH gene. The genotype file we used is extracted from <a href="https://www.1000genomes.org/">1000 Genomes Project</a>. It contains 85 individuals in <a href="https://www.internationalgenome.org/faq/can-i-get-phenotype-gender-and-family-relationship-information-samples/">CEU population</a> and the variants are all located in the CFH gene region.  We will use simulated phenotype in this demonstration.</p>

<pre><code class="r"># Extract genotype matrix
vcf.1000g &lt;- system.file(&quo