arately. EST assembly is also complicated by features like (cis-) <a href="//en.wikipedia.org/wiki/Alternative_splicing" title="Alternative splicing">alternative splicing</a>, <a href="//en.wikipedia.org/wiki/Trans-splicing" title="Trans-splicing">trans-splicing</a>, <a href="//en.wikipedia.org/wiki/Single-nucleotide_polymorphism" title="Single-nucleotide polymorphism">single-nucleotide polymorphism</a>, <a href="//en.wikipedia.org/wiki/Recoding" title="Recoding" class="mw-redirect">recoding</a>, and <a hre