ss="downlit sourceCode r">
<code class="sourceCode R"><span class="kw"><a href="https://rdrr.io/r/base/library.html">library</a></span><span class="op">(</span><span class="va"><a href="https://satijalab.org/seurat">Seurat</a></span><span class="op">)</span></code></pre></div>
<p>Create a Seurat object, and then perform SCTransform normalization. Note:</p>
<ul>
<li>You can use the legacy functions here (i.e., NormalizeData, ScaleData, etc.), use SCTransform or any other normalization method (including no no