 methylated (5mC) and the
cytosine in the fifth position is canonical (-), indicating a "hemi-methylation".</p>
<p>In the case of 5mC and canonical, there are 4 "patterns" of methylation:</p>
<pre><code class="language-text">m,m (5mC, 5mC)
-,m (canonical, 5mC)
m,- (5mC, canonical)
-,- (canonical, canonical)
</code></pre>
<p>These are all measured at the <em>single molecule</em> level, meaning each molecule must report on both strands (as
is the case with <a href="https://www.youtube.com/watch?v=8DVMG7FEBys"