rmalization). We did not notice a significant difference in cell type annotations with different normalization methods.</li>
<li>We think that it is best practice to use SCTransform, but it is not a necessary step. Signac will work fine without it.</li>
</ul>
<div class="sourceCode" id="cb4"><pre class="downlit sourceCode r">
<code class="sourceCode R"><span class="co"># load data</span>
<span class="va">kidney</span> <span class="op">&lt;-</span> <span class="fu"><a href="https://satijalab.org/seurat/refer