of cell differentiation, and trajectory inference is one of the key computational challenges in single-cell transcriptomics. In recent years, many software packages have been developed and widely used to extract an underlying “tree” trajectory from single-cell RNA-seq data.</p>
<p>However, trajectory inference often suffers from the uncertainty due to the heterogeneity of individual cells and the high levels of technical noise in single-cell experiments. Hence, it was a fatal problem that there was no m