rporated into micelles when the Tween-20&#226;&#132;&#162; concentration is higher than the CMC, for instance at 0.1 % in PBS at pH 7.4.
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<br>Normal sera are used to counteract the non-specific interaction between the sample and immunoglobulins. They can be added to the blocking solution and the incubation media.
<br>As a rule the normal serum species should be the same as the secondary antibody species (e.g. use normal goat serum with goat-anti-rabbit conjugates).
<br>Note: normal sera should not be us