" class="section level2">
<h2>Introduction</h2>
<p>This pipeline is designed for filtering mutations found in
formalin-fixed and paraffin-embedded (FFPE) samples. The MicroSEC filter
utilizes a statistical analysis, and the results for mutations with less
than 10 supporting reads are not reliable. Four files are necessary for
the analysis: mutation information file, BAM file, and mutation
supporting read ID information file.</p>
</div>
<div id="file-1-mutation-information-file-mandatory" class="section leve