Western blot
Microfluidic western blot

In order to detect many proteins on a single microfluidic chip, microfluidic western blot is carried out using a number of processes, including sample enrichment, protein size, deposition of protein, and then in situ antibody probing. A photoreactive (UV light) polyacrylamide gel and a photopatternable (blue-light) surface are the foundation of this multistep procedure. Due to improvements in analytical performance, WB may now be completed in 10–60 minutes while maintaining high sensitivity detection limits (50 picomoles) and multiplexed component detection levels (femtograms). Therefore, by fusing superb specificity and the high-throughput benefits of multiplexing, WB creates a cornerstone for quick proteomics.
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