Mass–energy equivalence
Application to nuclear physics

The nuclear binding energy is the minimum energy that is required to disassemble the nucleus of an atom into its component parts. The mass of an atom is less than the sum of the masses of its constituents due to the attraction of the strong nuclear force. The difference between the two masses is called the mass defect and is related to the binding energy through Einstein's formula. The principle is used in modeling nuclear fission reactions, and it implies that a great amount of energy can be released by the nuclear fission chain reactions used in both nuclear weapons and nuclear power.

A water molecule weighs a little less than two free hydrogen atoms and an oxygen atom. The minuscule mass difference is the energy needed to split the molecule into three individual atoms (divided by c), which was given off as heat when the molecule formed (this heat had mass). Similarly, a stick of dynamite in theory weighs a little bit more than the fragments after the explosion; in this case the mass difference is the energy and heat that is released when the dynamite explodes. Such a change in mass may only happen when the system is open, and the energy and mass are allowed to escape. Thus, if a stick of dynamite is blown up in a hermetically sealed chamber, the mass of the chamber and fragments, the heat, sound, and light would still be equal to the original mass of the chamber and dynamite. If sitting on a scale, the weight and mass would not change. This would in theory also happen even with a nuclear bomb, if it could be kept in an ideal box of infinite strength, which did not rupture or pass radiation. Thus, a 21.5 kiloton (9 × 10 joule) nuclear bomb produces about one gram of heat and electromagnetic radiation, but the mass of this energy would not be detectable in an exploded bomb in an ideal box sitting on a scale; instead, the contents of the box would be heated to millions of degrees without changing total mass and weight. If a transparent window passing only electromagnetic radiation were opened in such an ideal box after the explosion, and a beam of X-rays and other lower-energy light allowed to escape the box, it would eventually be found to weigh one gram less than it had before the explosion. This weight loss and mass loss would happen as the box was cooled by this process, to room temperature. However, any surrounding mass that absorbed the X-rays (and other "heat") would gain this gram of mass from the resulting heating, thus, in this case, the mass "loss" would represent merely its relocation.
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