Cytochalasin B
1970s

In the 1970s, research on the mitosis of polynucleated cells was done. It appeared that these cells were created through progressive nuclear addition instead of nuclear division. The process by which this occurs is called pseudomitosis, which is the synchronous mitosis resulting in the division of just one nucleus. The separate nuclei are bound by a nuclear bridge and in binucleated cells the centrioles are doubled.Furthermore, it was found that CB causes the disorganization of the 50Å microfilaments of mouse epithelial cells which causes the cells to lose their shape. It also affects the appearance of young glands in cells and new gland formation in other cells.Another group found that CB inhibits the ability of HeLa cells to undergo cytokinesis by decomposition of the contractile ring. Research from 1971 showed that CB interferes with the release of iodine derived from thyroglobulin and blocks colloid endocytosis. Moreover, it was found that CB has an inhibitory effect on the uptake of sucrose- 3 H by chang-strain human liver cells and in CB-treated cells alterations in the appearance and location of microfilaments were observed. Furthermore, it was found that CB reversibly inhibits melanin granule movement in melanocytes. One year later, research on the influence of cytochalasin B on chloroplasts was done. It was found that the light-oriented movement of chloroplasts is reversibly inhibited by cytochalasin B. In 1973 researches found that cytochalasin B is a powerful non-competitive inhibitor of glucose transport. One of the major electrophoretic identifiable erythrocyte membrane proteins may be the cytochalasin B binding site of erythrocytes.
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