stylistic:tone, structural:expansion
Repetition of the opening motif throughout the symphony

It is commonly asserted that the opening four-note rhythmic motif (short-short-short-long; see above) is repeated throughout the symphony, unifying it. "It is a rhythmic pattern (dit-dit-dit-dot) that makes its appearance in each of the other three movements and thus contributes to the overall unity of the symphony" (Doug Briscoe); "a single motif that unifies the entire work" (Peter Gutmann); "the key motif of the entire symphony"; "the rhythm of the famous opening figure... recurs at crucial points in later movements" (Richard Bratby). The New Grove encyclopedia cautiously endorses this view, reporting that "he famous opening motif is to be heard in almost every bar of the first movement—and, allowing for modifications, in the other movements."

There are several passages in the symphony that have led to this view. For instance, in the third movement the horns play the following solo in which the short-short-short-long pattern occurs repeatedly:

In the second movement, an accompanying line plays a similar rhythm:

In the finale, Doug Briscoe suggests that the motif may be heard in the piccolo part, presumably meaning the following passage:

Later, in the coda of the finale, the bass instruments repeatedly play the following:

On the other hand, some commentators are unimpressed with these resemblances and consider them to be accidental. Antony Hopkins, discussing the theme in the scherzo, says "no musician with an ounce of feeling could confuse ", explaining that the scherzo rhythm begins on a strong musical beat whereas the first-movement theme begins on a weak one. Donald Tovey pours scorn on the idea that a rhythmic motif unifies the symphony: "This profound discovery was supposed to reveal an unsuspected unity in the work, but it does not seem to have been carried far enough." Applied consistently, he continues, the same approach would lead to the conclusion that many other works by Beethoven are also "unified" with this symphony, as the motif appears in the "Appassionata" piano sonata, the Fourth Piano Concerto (), and in the String Quartet, Op. 74. Tovey concludes, "the simple truth is that Beethoven could not do without just such purely rhythmic figures at this stage of his art."

To Tovey's objection can be added the prominence of the short-short-short-long rhythmic figure in earlier works by Beethoven's older Classical contemporaries such as Haydn and Mozart. To give just two examples, it is found in Haydn's "Miracle" Symphony, No. 96 () and in Mozart's Piano Concerto No. 25, K. 503 (). Such examples show that "short-short-short-long" rhythms were a regular part of the musical language of the composers of Beethoven's day.

It seems likely that whether or not Beethoven deliberately, or unconsciously, wove a single rhythmic motif through the Fifth Symphony will (in Hopkins's words) "remain eternally open to debate".