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Juan Ponce de León (/ ˌ p ɒ n s d ə ˈ l iː ə n /, also UK: / ˌ p ɒ n s eɪ d ə l eɪ ˈ ɒ n /, US: / ˌ p ɒ n s d ə l i ˈ oʊ n, ˌ p ɒ n s (ə) d eɪ -/, Spanish:; 1474 – July 1521) was a Spanish explorer and conquistador known for leading the first official European expedition to Puerto Rico in 1508 and Florida in 1513. He was born in Santervás de Campos, Valladolid, Spain, in 1474. Though little is known about his family, he was of noble birth and served in the Spanish military from a young age. He first came to the Americas as a "gentleman volunteer" with Christopher Columbus 's second expedition in 1493.

By the early 1500s, Ponce de León was a top military official in the colonial government of Hispaniola, where he helped crush a rebellion of the native Taíno people. He was authorized to explore the neighboring island of Puerto Rico in 1508 and to take office as the first Governor of Puerto Rico by appointment of the Spanish crown in 1509. While Ponce de León grew quite wealthy from his plantations and mines, he faced an ongoing legal conflict with Diego Colón, the late Christopher Columbus's son, over the right to govern Puerto Rico. After a long court battle, Columbus replaced Ponce de León as governor in 1511. Ponce de León decided to follow the advice of the sympathetic King Ferdinand and explore more of the Caribbean Sea.

In 1513, Ponce de León led the first known European expedition to La Florida, which he named during his first voyage to the area. He landed somewhere along Florida's east coast, then charted the Atlantic coast down to the Florida Keys and north along the Gulf coast; historian John R. Swanton believed that he sailed perhaps as far as Apalachee Bay on Florida's western coast. Though in popular culture he was supposedly searching for the Fountain of Youth, there is no contemporary evidence to support the story, which most modern historians consider a myth.

Ponce de León returned to Spain in 1514 and was knighted by King Ferdinand, who also reinstated him as the governor of Puerto Rico and authorized him to settle Florida. He returned to the Caribbean in 1515, but plans to organize an expedition to Florida were delayed by the death of King Ferdinand in 1516, after which Ponce de León again traveled to Spain to defend his grants and titles. He did not return to Puerto Rico for two years.

In March 1521, Ponce de León finally returned to Southwest Florida with the first large-scale attempt to establish a Spanish colony in what is now the continental United States. However, the native Calusa people fiercely resisted the incursion, and Ponce de Léon was seriously wounded in a skirmish. The colonization attempt was abandoned, and he died from his wounds soon after returning to Cuba in early July. He was interred in Puerto Rico; his tomb is located inside the Cathedral of San Juan Bautista in San Juan.