information:highlighting, information:classification, stylistic:style_transfer, linguistic:translation
Reaction and Mechanism

The glucansucrase mechanism has historically been controversial in the scientific literature. The mechanism involves two displacements. The first originates from a glycosidic cleavage of the sucrose substrate between subsites -1 and +1. This releases fructose and forms a sugar-enzyme intermediate when the glucose unit attaches to the nucleophile.