structural:expansion
19th century

Speculation about life on Mars increased in the late 19th century, following telescopic observation of apparent Martian canals – which soon, however, turned out to be optical illusions. Despite this, in 1895, American astronomer Percival Lowell published his book Mars, followed by Mars and its Canals in 1906, proposing that the canals were the work of a long-gone civilisation.

Spectroscopic analysis of Mars's atmosphere began in earnest in 1894, when U.S. astronomer William Wallace Campbell showed that neither water nor oxygen was present in the Martian atmosphere. By 1909 better telescopes and the best perihelic opposition of Mars since 1877 conclusively put an end to the canal hypothesis.

As a consequence of the belief in the spontaneous generation there was little thought about the conditions of each celestial body: it was simply assumed that life would thrive anywhere. This theory was disproved by Louis Pasteur in the 19th century. Popular belief in thriving alien civilisations elsewhere in the solar system still remained strong until Mariner 4 and Mariner 9 provided close images of Mars, which debunked forever the idea of the existence of Martians and decreased the previous expectations of finding alien life in general. The end of the spontaneous generation belief forced to investigate the origin of life. Although abiogenesis is the more accepted theory, a number of authors reclaimed the term "panspermia" and proposed that life was brought to Earth from elsewhere. Some of those authors are Jöns Jacob Berzelius (1834), Kelvin (1871), Hermann von Helmholtz (1879) and, somewhat later, by Svante Arrhenius (1903).

The science fiction genre, although not so named during the time, developed during the late 19th century. The expansion of the genre of extraterrestrials in fiction influenced the popular perception over the real-life topic, making people eager to jump to conclusions about the discovery of aliens. Science marched at a slower pace, some discoveries fueled expectations and others dashed excessive hopes. For example, with the advent of telescopes, most structures seen on the Moon or Mars were immediately attributed to Selenites or Martians, and later ones (such as more powerful telescopes) revealed that all such discoveries were natural features. A famous case is the Cydonia region of Mars, first imaged by the Viking 1 orbiter. The low-resolution photos showed a rock formation that resembled a human face, but later spacecraft took photos in higher detail that showed that there was nothing special about the site.