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Single-cell western blot

The single-cell WB (scWB), in addition to the conventional WB, is regarded as a breakthrough in the study of protein subcellular localization and in the evaluation of single-cell protein. When measuring the levels and conditions of protein expression from one cell to the next, it is used. With the aid of single-cell WB, the western blot's selectivity and specificity were expanded to include single-cell protein analysis. The limitations of antibody accuracy and sensitivity are overcome by this technique. Furthermore, because of its versatility, it may be utilized to measure numerous target proteins concurrently from different cell lines and single cells.