structural:expansion, information:classification, stylistic:style_transfer, structural:summarization
Genome

The TMV genome consists of a 6.3–6.5 kbp single-stranded (ss) RNA. The 3’-terminus has a tRNA -like structure, and the 5’-terminus has a methylated nucleotide cap. (m7G5’pppG). The genome encodes 4 open reading frames (ORFs), two of which produce a single protein due to ribosomal readthrough of a leaky UAG stop codon. The 4 genes encode a replicase (with methyltransferase and RNA helicase domains), an RNA-dependent RNA polymerase, a so-called movement protein (MP) and a capsid protein (CP). The coding sequence starts with the first reading frame, which is 69 nucleotides away from the 5' end of the RNA. The noncoding region at the 5' end can be varied in different individual virions, but there hasn't been any variation found between virions in the noncoding region at the 3' end.