Predicted band size: 68 kDa . Positive control: Human liver cancer and fetal kidney tissue lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: Human liver cancer tissue lysate Lane2: Human fetal kidney tissue lysate Lysates: 40 ug per lane Primary antibody: 1/650 dilution Secondary antibody: Goat anti Rabbit IgG - H&amp;L (HRP) at 1/10000 dilution Exposure time: 90 seconds)