Subcellular Yap localization in organotypic bubble cultures. In utricles maintained for 4 d as organotypic bubble cultures in 640 Pa collagen gels (top panels), nuclear Yap labeling (green) occurs in Sox2-positive supporting cells (red) at the periphery of the macula. EdU incorporation (white) demonstrates cell proliferation. In similar preparations of the utricles maintained in 40 Pa gels (lower panels), nuclear Yap labeling and EdU incorporation occur in the Sox2-positive supporting cells throughout the sensory epithelium. The scale bar represents 25 μm. DOI: http://dx.doi.org/10.7554/eLife.25681.032 