Localization of myosin VI, V, and ezrin in A431 cells after stimulation with EGF. Cells were fixed at 0, 2, 5, and 15 min after addition of EGF and stained with rabbit polyclonal antibodies to myosin VI (affinity-purified PGT ab), myosin V (affinity-purified tail domain ab), and ezrin (affinity-purified ab) followed by fluorescently labeled anti–rabbit IgG. Extended focus projections of a z-series of images obtained by confocal microscopy are shown. Bar, 20 μm. 