Schematic and agarose gel electrophoresis of PCR amplification products. (A) Schematic representations of PCR amplicons from inverse PCR (upper) and cassette ligation PCR (lower). The theoretical minimum lengths including the tailing sequences (22 bp x 2) are 929 bp and 406 bp, respectively. (B) 1% agarose gel electrophoresis of inverse PCR (left) and cassette ligation PCR (right) products. Lane 0: non-transduced genomic DNA (negative control). A small proportion of bands (especially sample 5A) were smaller than the theoretical minimum lengths. (C) 2% agarose gel electrophoresis of PCR products after SmaI digestion. Arrows point to the expected specific bands. 