Figure 13. 
FAM83H and CK1ε immunocytochemistry. FLAG-tagged full-length (WT, FAM83H&lt;sup&gt;1-1209&lt;/sup&gt;) and truncated (697*, FAM83H&lt;sup&gt;1-697&lt;/sup&gt;) FAM83H proteins were expressed in HEK293 cells and immunostained with α-FLAG (green) and α-CK1ε (red) antibodies. Top: Both overexpressed WT FAM83H (green) and endogenous CK1ε (red) localize in the cytoplasm. Bottom: Unlike WT FAM83H, overexpressed FAM83H&lt;sup&gt;1-697&lt;/sup&gt; (green) localized in the nucleus. Endogenous CK1ε (red) also mislocalized to the nucleus. Panels on the right show the superimposition of FLAG and CK1ε images. Blue: DAPI nuclear staining.