document: visceral adipose tissue - derived serine proteinase inhibitor ( vaspin ) was identified in the visceral adipose tissue of oletf ( otsuka long - evans tokushim a fatty ) rats , an animal model of obesity and type 2 diabetes mellitus ( t2 dm ) . 
   vaspin is also expressed in the skin , hypothalamus , pancreatic islets , and stomach . 
   circulating levels of adiponectin , a hormone produced predominantly by adipocytes , are highly heritable and are inversely associated with t2 dm and other metabolic traits . 
   some studies have shown that plasma vaspin concentrations are significantly higher in men with the metabolic syndrome compared with those without the metabolic syndrome . 
   the serum vaspin levels in the patients with type 2 diabetes have been demonstrated to be higher than [ 5 , 6 ] or similar to   those observed in subjects with normal glucose tolerance . in recent , 
   inoue et al . reported that the serum vaspin levels are negatively correlated with the creatinine levels and were significantly reduced in the japanese chronic hemodialysis ( hd ) patients . 
   thus , it was interesting to ask whether serum vaspin levels are elevated or reduced in diabetic patients with renal insufficiency . 
   we measured serum vaspin and adiponectin levels in subjects with normal renal function and renal insufficiency with or without t2 dm and compared various clinical parameters . 
   all subjects were local residents of han ethnicity in shanghai and consecutively recruited in department of endocrinology and cardiology , xinhua hospital and shanghai tenth people 's hospital from june 2011 to december 2011 . 
   the study was approved by the ethical committee of shanghai tenth people 's hospital and xinhua hospital . 
   clinical characteristics were recorded with respect to age , sex , body mass index ( bmi ) , risk factors for renal function , and diabetic mellitus ( dm ) . 
   dm was defined as fasting serum glucose level 7.0  mmol / l or 2-hour postprandial glucose 11.1  mmol / l , or taking hypoglycemic drugs . those with type 1 diabetes , acute myocardial infarction , systemic inflammatory disorders , severe heart failure ( left ventricular ejection fraction < 30% ) , advanced renal insufficiency , and malignant tumor were excluded . 
   human total adiponectin ( r&d systems , minneapolis , american ) and vaspin ( adipogen , seoul , south korea ) plasma levels were measured with commercially available elisas as indicated before . while high - sensitive c - reactive protein ( hscrp ) , fibrinogen ( fib ) , creatinine ( cr ) , blood urea nitrogen ( bun ) , uric acid ( ua ) , fasting blood glucose ( fbg ) , 2-hour postprandial glucose ( pg2h ) , hemoglobin a1c ( hba1c ) , and lipid profiles , including total cholesterol ( tc ) , triglyceride ( tg ) , high - density lipoprotein cholesterol ( hdl - c ) , low - density lipoprotein cholesterol ( ldl - c ) , apolipoprotein a ( apoa ) , and apolipoprotein b ( apob ) were measured by colorimetric enzymatic assay systems ( roche modular p-800 , swiss confederation ) . 
   416 subjects were divided into 4 groups according to the presence or absence of ri and t2 dm : 247 individuals without t2 dm who had ri ( n = 27 ; group i ) or normal renal function ( n = 220 ; group ii ) and 169 patients with t2 dm who had ri ( n = 23 ; group iii ) or normal renal function ( n = 146 ; group iv ) . 
   renal function was assessed by the estimated creatinine clearance ( crcl ) derived from cockroft - gault formula , where crcl ( ml / min ) = ( [ 140  age ( years ) ]  weight ( kg))/(0.818  serum creatinine ( mol / l ) ) , corrected in women by a factor of 0.85 . 
   a calculated crcl less than 60  ml / min was defined as ri , according to guidelines . 
   statistical analyses were performed using the pasw statistics 20 software ( ibm corporation , armonk , ny , usa ) . 
   comparisons of variables with a normal distribution were made using student 's t - test , and values were provided as mean  s.d . for parameters with an abnormal distribution , the mann - whitney u test was used for comparisons , and values were given as median ( interquartile range ) . 
   categorical variables were presented as frequencies . to evaluate the correlation between serum vaspin and adiponectin levels with other variables such as age , bmi , hscrp , crcl , cr , fbg , and tc single linear univariate correlations and multivariate regression analyses were performed . 
   in the population either with t2 dm or without t2 dm , patients with ri ( group i or group iii ) were older and had lower bmi , higher systolic blood pressure ( sbp ) than those without ri ( group ii or group iv ) ( all p < 0.05 ) ( table 1 ) . 
   the serum vaspin showed no significant differences either between non - t2 dm patients with ri ( group i ) and without ri ( group ii ) ( 0.548 ( 0.4281.136 ) ng / ml versus 0.517 ( 0.2321.324 ) ng / ml , p = 0.402 ) or between diabetic patients with ri ( group iii ) and without ( group iv ) ( 0.497 ( 0.2880.907 )  ng / ml versus 0.381 ( 0.2320.740 )  ng / ml , p = 0.245 ) . 
   interestingly , the serum vaspin was lower in 169 patients with t2 dm than in 247 individuals without t2 dm ( 0.399 ( 0.2240.737  ng / ml versus 0.523 ( 0.1821.172 )  ng / ml , p < 0.05 ) ( table 2 ) 
   . however , no significant difference for the serum vaspin level was observed between 50 patients with ri ( combined by group i and group iii ) and 366 individuals without ri ( combined by group ii and group iv ) ( 0.540 ( 0.3991.020 )  ng / ml versus 0.432 ( 0.2340.925 )  ng / ml , p = 0.141 ) ( table 2 ) . 
   compared with the nondiabetic individuals without ri ( group ii ) , the serum adiponectin was higher in the non - diabetic individuals with ri ( group i ) ( 16082.437  9926.203 
   ng / ml , p < 0.01 ) but did not differ between diabetic patients with ri ( group iii ) and without ri ( group iv ) ( 12987.336  14623.762 
   ng / ml versus 8744.920  5628.952  ng / ml , p = 0.349 ) . 
   regardless of the individuals with or without t2 dm , the serum adiponectin level was significantly higher in 50 individuals with ri ( combined by group i and iii ) than 366 individuals without ri ( combined by group ii and iv ) ( 10549.706 ( 5589.71822429.025 )  ng / ml versus 6955.130 ( 2590.05213533.955 )  ng / ml , p < 0.01 ) ( tables 1 and 2 ) . 
   when compared with the individuals without t2 dm ( combined by group i and ii ) in our tall 416 samples , the serum adiponectin was significantly higher in 169 patients with t2 dm ( combined by group iii and iv ) ( 7599.068 ( 4100.63313827.261 )  ng / ml versus 5553.476 ( 12.09614225.162 )  ng / ml , p < 0.05 ) . 
   spearmen 's analysis showed that serum vaspin level was only related to cr in patients without t2 dm . in patients with t2 dm , 
   serum vaspin levels were related to age , sbp , and ua . in all subjects , serum vaspin levels were related to crcl , tc , and hba1c ( tables 3 and 4 ) . 
   the serum adiponectin levels were related to age , sbp , hscrp , crcl , and cr in subjects without t2 dm . in patients with t2 dm , 
   serum adiponectin levels were related to fbg , ldl - c . in all subjects , 
   serum adiponectin levels were related to age , sbp , crcl , cr , bun , and hba1c ( tables 3 and 4 ) . 
   multiple regression analyses were performed to test whether any of the following factors influence the serum vaspin and adiponectin concentrations : age , sbp , fbg , ua , crcl , cr , tg , tc , hba1c levels , and so on . when correcting for these variables using stepwise regression analyses after adjustment for age and bmi , the level of hba1c was found to be independently and significantly associated with the vaspin concentration in 427 total subjects . 
   however , no significant correlations between the serum vaspin level and any parameter were detected in both groups of the subjects with t2 dm and without t2 dm . 
   the level of crcl was found to be independently and significantly associated with the adiponectin concentration in all subjects . 
   however , in the groups of the subjects with or without t2 dm , no significant correlations were detected between the serum adiponectin level and any parameter ( tables 5 and 6 ) . 
   mann - whitney u test showed that the serum vaspin and adiponectin levels were both higher in women than in men whether in total subjects or in patients with t2 dm ( tables 7 and 8) . 
   adipose tissue is a highly active endocrine organ secreting a number of bioactive molecules called adipokines [ 5 , 6 , 12 ] , such as vaspin and adiponectin . 
   vaspin is a member serpin a12 of the serine protease inhibitor family [ 1315 ] , and a large number of clinical data have showed that vaspin played an important role in occurrence and development in the metabolic syndrome [ 1 , 16 , 17 ] . 
   upregulation of vaspin can improve the insulin resistance , which also inhibit inflammation and progression of arthrosclerosis . 
   some studies show that vaspin increases nitric oxide bioavailability through the reduction of asymmetric dimethylarginine in vascular endothelial cells   or inhibits platelet - derived growth factor - bb - induced migration of vascular smooth muscle cells . 
   the decrease of vaspin in the ri patients will increase the risk of coronary heart disease ( chd ) . 
   therefore , measuring the levels of adipokines in patients with ri may be beneficial for predicting cardiovascular events and patient survival . in patients with ckd , the levels of adipokines appear to increase in association with declines in the gfr . 
   this is most likely due to reduced renal metabolism of adipokines , which may not increase vascular risks in patients with a reduced renal function . 
   since vaspin is a small protein size with the molecular mass 50  kda , which would be freely filtered by the kidneys ; thus it is reasonable to presume that vaspin levels should be increased in patients with renal insufficiency . 
   but our data suggested that the serum vaspin levels have no significant difference between patients with ri and subjects without ri due to the fact that many factors might affect the serum levels of vaspin concentration . 
   previous studies have shown that , in the diabetic patients , serum vaspin level is higher than that in normal glucose tolerance subjects in women , and rosiglitazone therapy decreased plasma vaspin levels through glucose and insulin sensitivity regulation . 
   our study also shows that the serum vaspin level was higher in women than in men . 
   however , we have found that the serum vaspin level was lower in 169 diabetic patients than 247 individuals without t2 dm . 
   the paradox results may be caused by two reasons : one is the sex difference in the subjects between their study and our study ; another reason is that there are differences in the diabetic patients with vascular complications between the two population , since some reports have demonstrated that low vaspin serum concentrations have been found to be correlated with recently experienced ischemic events in patients with carotid stenosis   and coronary artery disease ( cad ) . 
   adiponectin is a fat - derived hormone whose reduction plays central roles in obesity - linked diseases including insulin resistance , type 2 diabetes , and atherosclerosis . 
   adiponectin multimers are secreted by white adipose tissue [ 2527 ] , which is associated with body weight and markers of cardiovascular risk in adolescence . 
   adiponectin has recently attracted much attention because of its antidiabetic and antiatherogenic effects as well as its antiproliferative effects in cancer cells ; thus , adiponectin is expected to be a therapeutic tool for diabetes , metabolic syndrome , cardiovascular diseases , and cancers [ 29 , 30 ] . 
   plasma adiponectin levels have also been reported to be reduced in obese humans , particularly those with visceral obesity [ 31 , 32 ] . 
   so , in the ri patients , lower bmi may lead to the high level of adiponectin 
   but in the diabetic patients , there are no differences between ri and non - ri . 
   some studies have shown that rosiglitazone can increase serum adiponectin levels in patients with type 2 diabetes mellitus [ 33 , 34 ] . in all patients with diabetes in our current study , only 19 patients have been treated with thiazolidinediones . after removing the patients , 
   both of t2 dm and renal insufficiency were correlated with the serum level of adiponectin . 
   although the serum vaspin levels showed no significant difference between the patients with renal insufficiency which did not required treatment by chronic hemodialysis and subjects without renal insufficiency , we have found that the serum vaspin level was lower in 169 diabetic patients than in 247 individuals without t2 dm .
summary: vaspin and adiponectin are two adipocytokines with antidiabetic effects . 
   some studies reported that levels of adiponectin and vaspin were correlated with decreased glomerular filtration rate ( fgr ) and increased albuminuria . 
   we therefore evaluated the vaspin and adiponectin levels in renal insufficiency ( ri ) patients with or without t2 dm . 
   serum vaspin , adiponectin levels were measured in 416 subjects with or without t2 dm . 
   analysis was made between groups divided by these subjects presence or absence of ri . 
   we found that serum adiponectin level was significantly higher in nondiabetic patients with ri than in nondiabetic subjects without ri ; however , there were no statistical differences between the diabetic patients with ri and without ri . 
   in all the subjects , the serum adiponectin level was also higher in 50 individuals with ri than that in 366 subjects without ri . 
   the serum vaspin levels showed no significant differences between the diabetic patients or nondiabetics subjects with ri and without ri . 
   contrary to adiponectin , the serum vaspin level was lower in 169 patients with t2 dm than in 247 individuals without t2 dm . 
   our data suggested that both of t2 dm and renal insufficiency were correlated with the serum level of adiponectin . 
   however , the serum vaspin levels showed no significant difference between the individuals with renal insufficiency and without renal insufficiency .