document: the transobturator approach to treat female stress urinary incontinence by means of a midurethral sling ( mus ) placement was first described by delorme to potentially prevent complications associated with the retropubic mus placement originally described by ulmsten ( delorme , 2001 ; ulmsten et al . , 1996 ) . 
   a similar transobturator passage utilizing an inside - out approach , as opposed to delorme s outside - in approach , was later described by de leval , aiming to further reduce the potential injury to the urethra and bladder ( de leval , 2003 ) . 
   a recent meta - analysis found similar cure rates between the 3 most commonly used surgical approaches for treating stress urinary incontinence , namely the retropubic , outside - in transobturator , and inside - out transobturator mus procedures ( latthe et al . , 2010 ) . 
   multiple studies have discussed the anatomical trajectories of both obturator approaches and confirmed their anatomical safety from a neurovascular perspective ( bonnet et al . , 2005 ; achtari et al . , 2006 ; hinoul et al . 
   , 2007 ; zahn et al . , 2007 ; reisenauer et al . , 2006 ) . 
   the large body of clinical evidence supports these findings . to date , anatomical studies have focused on the mus s trajectory in relation to the obturator foramen , the muscles and especially the neuro - vascular structures . 
   traditional anatomical dissections do not allow the study of the mus s configuration in relation to the urethra , as a progressive dissection of the entire tape s trajectory would be required , inherently leading to a distortion of the relevant anatomy . 
   the aim of this cadaveric study was to compare the specific relationship between the tape and the urethra in both the inside - out and outside - in approach . to allow such a comparison , 
   ct - scan image analysis was performed after tantalum wire marked mesh slings were implanted in a series of cadavers . 
   all 10 cadavers were operated upon , by independent surgeons , one experienced with the  inside - out ( in - out ) and one with 
   cadavers were positioned in the dorsal supine lithotomy position with the legs in abduction and at a 100 flexion position ( at the level of the hips ) on the in - outside and at 90 flexion on the out - in side . the surgical technique for the transobturator mus procedures were performed according to the instructions for use as provided by the respective manufacturers ( out - in : monarc , american medical systems , minnetonka , mn and in - out : tvt - obturator system , ethicon inc . , 
   all cadavers were catheterized using a foley catheter with its lumen filled with a radio - opaque solution ( hypaquemeglumine 60% , amersham health , princeton , nj ) to allow subsequent identification of the urethral position . 
   both the in - out and the out - in transobturator procedures were performed on each cadaver , alternating the type of procedure and tape between the right and left sides in the consecutively operated cadavers . to be able to do this with a single tape in each cadaver , 
   a device was conceived for this study in which the tape on one side consisted of an in - out mus device , while an out - in mus device was attached to the other side at the midpoint . 
   to allow radiographic visualization of these tapes , a double tantalum wire was threaded through each side of both midurethral tapes as shown in figure 1 . 
   this design allowed for an intra - cadaveric comparison between 10 independent in - out transobturator mus trajectories and 10 out - in transobturator mus trajectories . 
   care was taken to have a flat positioning of both tape ends at the end of the procedures . 
   the vaginal incision sites were closed and the mus tapes were cut 2  cm from the level of the skin . to exclude the possible influence of the surgeon s right handedness , an equal number of both procedures was performed on the right and left sides ; 
   bmi of the cadavers ranged between 15 and 33  kg / m2 ( mean : 25.3 ; median : 26 ) . 
   post capture image analysis was performed using the vitrea core 5.1.1618.1808 image analysis software ( vital , 5850 opus parkway , suite 300 , minnetonka , mn 55343 - 4414 ) . 
   plane that included the urethra at the point where it crossed the urethra and the two arms of the sling extending from the urethra to the point where it crossed the obturator membrane . 
   this was done to prevent parallax effects that might alter the measured angle ( hoyte and ratiu , 2001 ) . 
   on the mips view of the images , the relative angulations between a line through the urethral axis and the slope drawn through each mus tape s mean upward pathway ( forming the hammock part of its trajectory ) were measured ( table i ) . 
   the angle inside the upward trajectory of the tape constituted the angle under consideration for this study and is referred to as the tape s hemi inner angle , as only half a procedure was performed on each cadaver ( fig . 
   for the study s purposes the assumption was made that a symmetric , bilateral placement of a full length tape would yield the full inner angle of the mus hammock corresponding to double the hemi - inner angle measurement . 
   2a . ventro - dorsal view ( frontal view ; l = left , r = right ) . 
   note : dotted lines schematically demonstrates the hemi - inner angle but do not correspond to the mips view used to measure the actual angles . 
   statistical analyses were performed using the wilcoxon signed - rank test for matched paired data for non - parametric testing of 2 dependent samples . 
   statistical analyses were performed using the wilcoxon signed - rank test for matched paired data for non - parametric testing of 2 dependent samples . 
   three cadavers were noted to have stage 2 prolapse and 1 had a notably high vaginal sulcus . 
   the 3-dimensional ct images revealed that the mus tapes lie as a band posterior / dorsal to the urethra rather than inferior . 
   the out - in muss were more closely wrapped around the inferior ischio - pubic ramus than the in - out muss . 
   qualitative assessment of the images demonstrated a safe distance from the tapes to the obturator canal for both techniques . 
   distances of the tapes trajectories to the individual obturator nerve branches could not be determined . 
   the mean full inner angle in a strict antero - posterior view formed by the in - out mus measured 122 ( 95%ci : 107-136 ) ; versus 144 ( 95%ci : 131-151 ) for the out - in mus ( p  =  0.02 ) . 
   the ( paired ) differences between the tapes inner angles were significantly different ; with a mean difference of 19.8 ( median 19.0 ) , ( wilcoxon signed rank test : p  =   0.008 ) . 
   correlation analysis only showed a trend between the out - in and the in - out mus angle ( correlation coefficient : 0.6 , p  =  0.08 ) . 
   a similar trend was observed between the patient s bmi and the in - out mus angle ( correlation coefficient : 0.6 , p  =  0.08 ) , but not for bmi and the out - in mus angle ( correlation coefficient : 0.08 , p  =  0.8 ) . 
   there was no correlation between the infra - pubic angle measurement and the out - in mus angle nor the in - out mus angle . 
   the use of tantalum wires threaded through the polypropylene-/ prolene - tape in combination with three - dimensional ct - scanning yielded a unique method to visualize midurethral tapes throughout the entirety of their trajectory . 
   this technique can be applied to different types of non radio - opaque implants , allowing for a better spatial understanding of the anatomical position within the pelvis . 
   this technique circumvents the technical problems encountered by ultrasound where visualization is partially obstructed by the bony pelvis and the fact that traditional anatomical techniques rely on a progressive dissection intrinsically only allowing visualization at a single level each time . 
   this is the first controlled anatomical study to compare the spatial relationships between the in - out and out - in mus tapes and the female urethra . 
   the difference could be objectified by measuring the angle formed between the two legs of the mus tape . 
   the in - out approach angulated around the urethra in a more acute fashion than the out - in approach , which seemed to follow a more horizontal , flatter , trajectory ( 122 versus 142 ) . 
   these findings were counter - intuitive as the exit - points of both devices at the level of the skin would suggest the opposite . 
   the three dimensional radiographic visualization of the tape s trajectory also allowed for an appreciation of the mus tapes spatial configuration in relation to the bony pelvis and the urethra . 
   contrary to the belief that the urethra is suspended in a cranially directed u - shaped band , both obturator mus approaches were demonstrated to be u - shaped bands that head backwards or dorsally . 
   the tape is not positioned caudally but lies as a band behind the urethra , suggestive of its passive ( non obstructive ) role in achieving continence . as observed in the different measurements of the tapes angles , there seemed to be a higher variability in the in - out s than the out - in trajectory ( 58 versus 40 variation respectively ) , as previously described by hinoul et al . in a traditional anatomic study , underscoring the need for strict adherence to the prescribed operative technique ( hinoul et al . , 2007 ) . 
   the importance of this becomes clear when comparing it to two ultrasound studies investigating the angles formed by the two arms of mus tapes . 
   lin et al described an angle at rest for the in - out tape to be 115 ( sd13 ) while chene et al , measured the same angle at rest also using ultrasound to be 138(sd7 ) ( lin et al . 
   the differences in outcomes obtained in these studies demonstrate that ( inter- and intra - individual ) comparisons may prove to be difficult to interpret . 
   the cadaveric study design was also the study s limitation as no clinical findings could be correlated to the static anatomic findings . a direct meta - analysis comparing both routes of transobturator tapes by madhuvrata et al . 
   ( 2012 ) reported no significant differences in the objective and subjective efficacy but showed that the  inside - out 
   route was associated with significantly fewer vaginal angle injuries but with a non - significanttrend towards higher risk of postoperative groin pain . in a small retrospective study , out - in mus tapes 
   were more frequently associated with a finding of superficial placement at the level of the lateral vaginal sulcus , described as paraurethral banding ( cholhan et al . , 2010 ) . 
   coincidentally , the only cadaver with a notably high vaginal sulcus was found to have a very palpable out - in mus tape post placement . increased rates of dyspareunia and tape exposures after the out - in compared to the in - out approach 
   ( 2012 ) , but these finding were not confirmed in an rct by abdel - fattah et al . 
   the more horizontal positioning of the out - in tape s trajectory , taking the mesh closer to the vaginal wall , as described in this study could explain these clinical observations . 
   the radiographic images explain the increased risk of  button - holing during an out - in procedure because the more acute in - out mus trajectory buries the tape deeper inside the internal adductor muscles . on the other hand 
   , this study also confirmed that the out - in mus trajectory stayed closer to the ischiopubic ramus . 
   the slightly more lateral in - out mus trajectory causes the tape to be seated in more muscular tissue . 
   the corresponding inflammatory reaction might explain the trend to a slightly higher risk of transient thigh pain . 
   the clinical implications from this cadaveric imaging study are that surgeons must pay more attention to the 2nd half of the helical pass , whichever way they chose to go . for the in - out passage 
   , one must rotate hard and carefully around the ischiopubic ramus and not get lazy with the rotation ; while for the out - in pass , care must be taken that dissection of the peri - urethral tunnel provides for a full thickness vaginal epithelial layer . 
   the advent of three - dimensional ultrasonography will allow for similar measurements of the different tapes trajectories in vivo in a dynamic setting , possibly allowing correlation of these findings to nuances in clinical outcomes . 
   ultrasound will not be able to provide the same holistic visualization within the bony pelvis , but mesh - tape beyond the obturator membrane probably does not contribute to the mus functional effect or possible adverse events at the level of the vagina . 
   recently , a new approach , incorporating iron particles into polymer - based implants , allowed in vivo visualization of mesh by magnetic resonance imaging ( mri ) following inguinal hernia surgery ( hansen et al . , 2013 ) . 
   approval of these types of products for human use in the future will bring new insights into the action mechanism of mesh in the pelvic floor as well as a better understanding of the mesh- tissue interaction .
summary: the three - dimensional configuration of mid - urethral sling tapes is difficult to demonstrate in traditional anatomical dissections or imaging studies . the aim of this study was to test the utility of a novel technique using mesh tapes to assess spatial differences between the in - out and out - in transobturator mid - urethral slings . 
   two independent surgeons performed their usual transobturator mid - urethral sling placement on 10 fresh thawed cadavers , alternating sides in the consecutive cadavers . 
   tantalum wires threaded through the polypropylene - tapes rendered them radio - opaque . following placement , ct scans 
   were obtained to generate 3-d and mips images for analysis . 
   results showed that the mean angle formed by the in - out sling measured 122 ( 95%ci : 107-136 ) ; versus 144 ( 95%ci : 131-151 ) for the out - in sling ( p  =  0.02 ) . 
   the paired differences between the tapes inner angles were significantly different ; with a mean difference of 20 ( median 19.0 ) , ( p  =  0.008 ) . there was no significant correlation between either approach and bmi or angle of the pubic arch . 
   the images revealed that the tapes lie as a band posterior / dorsal to the urethra rather than inferior . in conclusion : marking mesh with tantalum wire , in combination with 3-d and mips ct - scan reconstruction images , provided a unique method to visualize the entire sling trajectory . 
   the clinical implications of the more horizontal positioning after the out - in approach remain to be determined .