<jupyter_start><jupyter_text>pYPK1pYPK1 is a version of [pYPK0](pYPK0.ipynb) that has a CEN6/ARS yeast origin of replicationinstead of the 2µ origin of replication present in pYPK0.The URA3 marker is substituted for a bleomycin marker.<jupyter_code>from pydna.all import *
pMEC1042 =read("pMEC1042.gb")
gb =Genbank("<EMAIL>")
pCAPs = gb.nucleotide("AJ001614.1")
from Bio.Restriction import PvuI, BamHI, EcoRI

pCAPs_pvu   = pCAPs.linearize(PvuI)
stuffer, pMEC1042_EB = pMEC1042.cut(BamHI, EcoRI)
pMEC1042_EB, stuffer
asm=Assembly([pMEC1042_EB, pCAPs_pvu], limit=245)
asm
candidate = asm.assemble_circular()[0]
candidate
pYPK1 = candidate.synced(pCAPs)
pYPK1.stamp()
pYPK1.locus = "pYPK1"
pYPK1.write("pYPK1.gb")<jupyter_output><empty_output><jupyter_text>[DOWNLOAD](pYPK1.gb)<jupyter_code>from pydna.all import *
reloaded=read("pYPK1.gb")
assert reloaded.cseguid() in reloaded.definition<jupyter_output><empty_output>