{| class="wikitable"
|-
! style="background:white"|Hold
! style="background:white"|By
! style="background:white"|Stadion
! style="background:white"|Kapicitet
! style="background:white"|Sluttede sig til MLS
! style="background:white"|Træner
|-
! style="background:#001F5B;" colspan="6"|[[Eastern Conference (MLS)|<span style="color:#FFFFFF;">Eastern Conference</span>]]
|-
| '''[[Atlanta United FC]]'''
| [[Atlanta|Atlanta, Georgia]]
| [[Mercedes-Benz Stadium]]
| align=center|42,500
| align=center|2017
| [[Gonzalo Pineda]]
|-
| '''[[Charlotte FC]]'''
| [[Charlotte (North Carolina)|Charlotte, North Carolina]]
| [[Bank of America Stadium]]
| align=center|38,000
| align=center|2022
| [[Miguel Ángel Ramírez]]
|-
| '''[[Chicago Fire|Chicago Fire FC]]'''
| [[Chicago|Chicago, Illinois]]
| [[Soldier Field]]
| align=center|24,995
| align=center|1998
| [[Ezra Hendrickson]]
|-
| '''[[FC Cincinnati]]'''
| [[Cincinnati|Cincinnati, Ohio]]
| [[TQL Stadium]]
| align=center|26,000
| align=center|2019
| [[Pat Noonan]]
|-
| '''[[Columbus Crew]]'''
| [[Columbus (Ohio)|Columbus, Ohio]]
| [[Lower.com Field]]
| align=center|20,011
| align=center|1996
| [[Caleb Porter]]
|-
| '''[[D.C. United]]'''
| [[Washington, D.C.]]
| [[Audi Field]]
| align=center|20,000
| align=center|1996
| [[Chad Ashton]] (interim)
|-
| '''[[Inter Miami CF]]'''
| [[Fort Lauderdale]], [[Florida]]
| [[DRV PNK Stadium]]
| align=center|18,000
| align=center|2020
| [[Phil Neville]]
|-
| '''[[CF Montréal]]'''
| [[Montreal|Montreal, Quebec]]
| [[Saputo Stadium]]
| align=center|19,619
| align=center|2012
| [[Wilfried Nancy]]
|-
| '''[[New England Revolution]]'''
| [[Foxborough, Massachusetts]]
| [[Gillette Stadium]]
| align=center|20,000
| align=center|1996
| [[Bruce Arena]]
|-
| '''[[New York City FC]]'''
| [[New York City|New York City, New York]]
| [[Yankee Stadium]]
| align=center|30,321
| align=center|2015
| [[Ronny Deila]]
|-
| '''[[New York Red Bulls]]'''
| [[Harrison, New Jersey]]
| [[Red Bull Arena (New Jersey)|Red Bull Arena]]
| align=center|25,000
| align=center|1996
| [[Gerhard Struber]]
|-
| '''[[Orlando City SC]]'''
| [[Orlando, Florida]]
| [[Exploria Stadium]]
| align=center|25,500
| align=center|2015
| [[Óscar Pareja]]
|-
| '''[[Philadelphia Union]]'''
| [[Chester, Pennsylvania]]
| [[Subaru Park]]
| align=center|18,500
| align=center|2010
| [[Jim Curtin]]
|-
| '''[[Toronto FC]]'''
| [[Toronto|Toronto, Ontario]]
| [[BMO Field]]
| align=center|28,351
| align=center|2007
| [[Bob Bradley]]
|-
! style="background:#E2231A;" colspan="6"|[[Western Conference (MLS)|<span style="color:#FFFFFF;">Western Conference</span>]]
|-
| '''[[Austin FC]]'''
| [[Austin, Texas]]
| [[Q2 Stadium]]
| align=center|20,500
| align=center|2021
| [[Josh Wolff]]
|-
| '''[[Colorado Rapids]]'''
| [[Commerce City, Colorado]]
| [[Dick's Sporting Goods Park]]
| align=center|18,061
| align=center|1996
| [[Robin Fraser]]
|-
| '''[[FC Dallas]]'''
| [[Frisco (Texas)|Frisco, Texas]]
| [[Toyota Stadium (Texas)|Toyota Stadium]]
| align=center|20,500
| align=center|1996
| [[Nico Estévez]]
|-
| '''[[Houston Dynamo FC]]'''
| [[Houston|Houston, Texas]]
| [[PNC Stadium]]
| align=center|22,039
| align=center|2006
| [[Paulo Nagamura]]
|-
| '''[[Nashville SC]]'''
| [[Nashville|Nashville, Tennessee]]
| [[Geodis Park]]
| align=center|30,000
| align=center|2020
| [[Gary Smith (footballer, born 1968)|Gary Smith]]
|-
| '''[[Sporting Kansas City]]'''
| [[Kansas City (Kansas)]]
| [[Children's Mercy Park]]
| align="center" |18,467
| align="center" |1996
| [[Peter Vermes]]
|-
| '''[[LA Galaxy]]'''
| [[Carson (California)|Carson, California]]
| [[Dignity Health Sports Park]]
| align=center|27,000
| align=center|1996
| [[Greg Vanney]]
|-
| '''[[Los Angeles FC]]'''
| [[Los Angeles|Los Angeles, California]]
| [[Banc of California Stadium]]
| align=center|22,000
| align=center|2018
| [[Steve Cherundolo]]
|-
| '''[[Minnesota United FC]]'''
| [[Saint Paul, Minnesota]]
| [[Allianz Field]]
| align=center|19,400
| align=center|2017
| [[Adrian Heath]]
|-
| '''[[Portland Timbers]]'''
| [[Portland, Oregon]]
| [[Providence Park]]
| align=center|25,218
| align=center|2011
| [[Giovanni Savarese]]
|-
| '''[[Real Salt Lake]]'''
| [[Sandy, Utah]]
| [[Rio Tinto Stadium]]
| align=center|20,213
| align=center|2005
| [[Pablo Mastroeni]]
|-
| '''[[San Jose Earthquakes]]'''
| [[San Jose (Californien)]]
| [[PayPal Park]]
| align=center|18,000
| align=center|1996
| [[Alex Covelo]] (interim)
|-
| '''[[Seattle Sounders FC]]'''
| [[Seattle|Seattle, Washington]]
| [[Lumen Field]]
| align=center|37,722
| align=center|2009
| [[Brian Schmetzer]]
|-
| '''[[Vancouver Whitecaps FC]]'''
| [[Vancouver|Vancouver, British Columbia]]
| [[BC Place]]
| align=center|22,120
| align=center|2011
| [[Vanni Sartini]]
|}
Historie Foreninger Organisationer