Instruct: Identify the main and secondary category of Arxiv papers based on the titles and abstracts
Query: Semiparametric point process modeling of blinking artifacts in PALM   Photoactivated localization microscopy (PALM) is a powerful imaging technique
for characterization of protein organization in biological cells. Due to the
stochastic blinking of fluorescent probes, and camera discretization effects,
each protein gives rise to a cluster of artificial observations. These blinking
artifacts are an obstacle for quantitative analysis of PALM data, and tools for
their correction are in high demand. We develop the Independent Blinking
Cluster point process (IBCpp) family of models, which is suited for modeling of
data from single-molecule localization microscopy modalities, and we present
results on the mark correlation function. We then construct the PALM-IBCpp - a
semiparametric IBCpp tailored for PALM data, and we describe a procedure for
estimation of parameters, which can be used without parametric assumptions on
the spatial organization of proteins. Our model is validated on nuclear pore
complex reference data, where the ground truth was accurately recovered, and we
demonstrate how the estimated blinking parameters can be used to perform a
blinking corrected test for protein clustering in a cell expressing the adaptor
protein LAT. Finally, we consider simulations with varying degrees of blinking
and protein clustering to shed light on the expected performance in a range of
realistic settings.

1
The categories of this paper are: cs
29
The categories of this paper are: funct-an
14
The categories of this paper are: math-ph
19
The categories of this paper are: eess
3
The categories of this paper are: physics
26
The categories of this paper are: bayes-an
10
The categories of this paper are: q-bio
20
The categories of this paper are: acc-phys
2
The categories of this paper are: math
25
The categories of this paper are: patt-sol
8
The categories of this paper are: hep-ex
28
The categories of this paper are: alg-geom
32
The categories of this paper are: atom-ph
7
The categories of this paper are: hep-th
23
The categories of this paper are: solv-int
37
The categories of this paper are: supr-con