Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Deficiency of intellectual disability-related gene Brpf1 reduced inhibitory neurotransmission and Map2k7 expression in GABAergic interneurons Intellectual disability is closely related to impaired GABA neurotransmission. Brpf1 was specifically expressed in medial ganglionic eminence (MGE), a developmental niche of GABAergic interneurons, and patients with BRPF1 mutations were mentally retarded. To test its role in development and function of MGE-derived GABAergic interneurons, we performed immunofluorescence staining, whole-cell patch-clamp, MGE transplantation and mRNA-Seq to understand its effect on neuronal differentiation, dendritic morphology, electrophysiology, migration and gene regulation, using mouse MGE-derived GABAergic interneurons infected with AAV-shBrpf1. We found a decreasing trend on parvalbumin+ interneuron differentiation. Moreover, increased firing threshold, decreased number of evoked APs, and a reduced amplitude of mIPSCs were observed before any significant change of MAP2+ dendritic morphology and in vivo migration appeared. Finally, mRNA-Seq analysis revealed that genes related to neurodevelopment and synaptic transmission such as Map2k7 were dysregulated. Our results demonstrated a key role of Brpf1 in inhibitory neurotransmission and related gene expression of GABAergic interneurons.
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The categories of this paper are: scientific
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The categories of this paper are: cancer
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The categories of this paper are: bioinformatics
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The categories of this paper are: biophysics
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The categories of this paper are: microbiology
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The categories of this paper are: biology
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The categories of this paper are: education
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The categories of this paper are: biochemistry
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The categories of this paper are: systems
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The categories of this paper are: developmental
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The categories of this paper are: immunology
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The categories of this paper are: pathology
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The categories of this paper are: toxicology
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The categories of this paper are: pharmacology
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The categories of this paper are: plant
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The categories of this paper are: and