Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: A Multi-omics Method Enabled by Sequential Metabolomics and Proteomics for Human Pluripotent Stem Cell-derived Cardiomyocytes Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) show immense promise for patient-specific disease modeling, cardiotoxicity screening, and regenerative therapy development. However, hPSC-CMs in culture have not recapitulated the structural or functional properties of adult CMs in vivo thus far. To gain global insight into hPSC-CM biology, we established a multi-omics method for analyzing the hPSC-CM metabolome and proteome from the same cell culture, creating multi-dimensional profiles of hPSC-CMs. Specifically, we developed a sequential extraction to capture metabolites and proteins from the same hPSC-CM monolayer cultures, and analyzed these extracts using high-resolution mass spectrometry (MS). Using this method, we annotated 205 metabolites/lipids and 4,008 proteins from 106 cells with high reproducibility. We further integrated the proteome and metabolome measurements to create network profiles of molecular phenotypes for hPSC-CMs. Out of 310 pathways identified using metabolomics and proteomics, 40 pathways were considered significantly overrepresented (FDR-corrected p [&le;] 0.05). Highly populated pathways included those involved in protein synthesis (ribosome, spliceosome), ATP generation (oxidative phosphorylation), and cardiac muscle contraction. This multi-omics method achieves deep coverage of metabolites and proteins, creating a multidimensional view of the hPSC-CM phenotype, which provides a strong technological foundation to advance the understanding of hPSC-CM biology.
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The categories of this paper are: paleontology
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The categories of this paper are: genetics
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The categories of this paper are: epidemiology
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The categories of this paper are: ecology
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The categories of this paper are: bioinformatics
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The categories of this paper are: bioengineering
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The categories of this paper are: and
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The categories of this paper are: molecular
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The categories of this paper are: genomics
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The categories of this paper are: cognition
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The categories of this paper are: cell
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The categories of this paper are: behavior
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The categories of this paper are: biochemistry
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The categories of this paper are: toxicology
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The categories of this paper are: developmental
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The categories of this paper are: scientific