Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Amnion signals are essential for mesoderm formation in primates Essential genes for murine embryonic development can demonstrate a disparate phenotype in human cohorts. By generating a transcriptional atlas containing >30,000 cells from postimplantation non-human primate embryos, we discovered that ISL1, a gene with a well-established role in cardiogenesis, controls a gene regulatory network in primate amnion. CRISPR/Cas9-targeting of ISL1 resulted in non-human primate embryos which did not yield viable offspring, demonstrating that ISL1 is critically required in primate embryogenesis. On a cellular level, mutant ISL1 embryos displayed a failure in mesoderm formation due to reduced BMP4 signaling from the amnion. Via loss of function and rescue studies in human embryonic stem cells we confirmed a similar role of ISL1 in human in vitro derived amnion. This study highlights the importance of the amnion as a signaling center during primate mesoderm formation and demonstrates the potential of in vitro primate model systems to dissect the genetics of early human embryonic development.
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The categories of this paper are: pharmacology
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The categories of this paper are: cancer
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The categories of this paper are: ecology
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The categories of this paper are: animal
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The categories of this paper are: physiology
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The categories of this paper are: biophysics
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The categories of this paper are: communication
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The categories of this paper are: biochemistry
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The categories of this paper are: scientific
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The categories of this paper are: molecular
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The categories of this paper are: bioinformatics
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The categories of this paper are: cell
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The categories of this paper are: zoology
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The categories of this paper are: education
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The categories of this paper are: microbiology
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The categories of this paper are: genetics