Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Cytoplasmic labile iron accumulates in aging stem cells perturbing a key rheostat for identity control Bone marrow resident and rarely dividing haematopoietic stem cells (HSC) harbour an extensive self-renewal capacity to sustain life-long blood formation;1-5 albeit their function declines during ageing.6, 7 Various molecular mechanisms confer stem cell identity, ensure long-term maintenance and are known to be deregulated in aged stem cells.8, 9 How these programs are coordinated, particularly during cell division, and what triggers their ageing-associated dysfunction has been unknown. Here, we demonstrate that HSC, containing the lowest amount of cytoplasmic chelatable iron (labile iron pool)10 among hematopoietic cells, activate a limited iron response during mitosis. Engagement of this iron homeostasis pathway elicits mobilization and {beta}-oxidation of arachidonic acid and enhances stem cell-defining transcriptional programs governed by histone acetyl transferase Tip60/KAT5. We further find an age-associated expansion of the labile iron pool, along with loss of Tip60/KAT5-dependent gene regulation to contribute to the functional decline of ageing HSC, which can be mitigated by iron chelation. Together, our work reveals cytoplasmic redox active iron as a novel rheostat in adult stem cells; it demonstrates a role for the intracellular labile iron pool in coordinating a cascade of molecular events which reinforces HSC identity during cell division and to drive stem cell ageing when perturbed. As loss of iron homeostasis is commonly observed in the elderly, we anticipate these findings to trigger further studies into understanding and therapeutic mitigation of labile iron pool-dependent stem cell dysfunction in a wide range of degenerative and malignant pathologies.
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The categories of this paper are: microbiology
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The categories of this paper are: physiology
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The categories of this paper are: ecology
6
The categories of this paper are: plant
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The categories of this paper are: paleontology
4
The categories of this paper are: neuroscience
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The categories of this paper are: systems
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The categories of this paper are: communication
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The categories of this paper are: epidemiology
1
The categories of this paper are: bioinformatics
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The categories of this paper are: cancer
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The categories of this paper are: genomics
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The categories of this paper are: pathology
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The categories of this paper are: toxicology
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The categories of this paper are: animal
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The categories of this paper are: immunology