Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Highly basic clusters in the HSV-1 nuclear egress complex drive membrane budding by inducing lipid ordering During replication of herpesviruses, capsids escape from the nucleus into the cytoplasm by budding at the inner nuclear membrane. This unusual process is mediated by the viral nuclear egress complex (NEC) that deforms the membrane around the capsid by oligomerizing into a hexagonal, membrane-bound scaffold. Here, we found that highly basic membrane-proximal regions (MPRs) of the NEC alter lipid order by inserting into the lipid headgroups and also promote negative Gaussian curvature. We also find that the electrostatic interactions between the MPRs and the membranes are essential for membrane deformation. One of the MPRs is phosphorylated by a viral kinase during infection, and the corresponding phosphomimicking mutations block capsid nuclear egress. We show that the same phosphomimicking mutations disrupt the NEC/membrane interactions and inhibit NEC-mediated budding in vitro, providing a biophysical explanation for the in-vivo phenomenon. Our data suggest that the NEC generates negative membrane curvature by both lipid ordering and protein scaffolding and that phosphorylation acts as an "off" switch that inhibits the membrane-budding activity of the NEC to prevent capsid-less budding.
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The categories of this paper are: genomics
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The categories of this paper are: pharmacology
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The categories of this paper are: paleontology
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The categories of this paper are: ecology
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The categories of this paper are: pathology
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The categories of this paper are: scientific
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The categories of this paper are: cognition
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The categories of this paper are: immunology
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The categories of this paper are: animal
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The categories of this paper are: bioengineering
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The categories of this paper are: and
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The categories of this paper are: cell
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The categories of this paper are: developmental
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The categories of this paper are: biochemistry
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The categories of this paper are: biophysics
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The categories of this paper are: communication