Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Structural insights as to how iron-loaded siderophores are imported into Mycobacterium tuberculosis by IrtAB The ATP-binding cassette (ABC) transporter, IrtAB, plays a vital role in the replication and viability of Mycobacterium tuberculosis (Mtb), where its function is to import iron-loaded siderophores. Unusually, it adopts the typical fold of canonical ABC exporters. Here, we report the structure of unliganded Mtb IrtAB and its structure in complex with ATP, ADP, and an ATP analogue (AMP-PNP) at resolutions from 2.9 to 3.5 [A]. In the ATP-bound state, the two nucleotide-binding domains (NBDs) form a "head-to-tail" dimer, but IrtAB has an unexpectedly occluded conformation, with the inner core forming a large hydrophilic cavity of about 4600 [A]3. Comparison of the structure of the transporter in inward-facing and occluded conformations reveals that the NBD and the intracellular helical region of transmembrane domain (TMD) have an asymmetric allosteric mechanism when ATP binding/hydrolysis such that the one exhibits rigid-body rotation and the other moves in a concerted response as a rigid body. This study provides a molecular basis for the ATP-driven conformational changes that occur in IrtAB and an explanation as to how iron-loaded siderophores are imported into Mtb by IrtAB.
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The categories of this paper are: biochemistry
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The categories of this paper are: pathology
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The categories of this paper are: developmental
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The categories of this paper are: animal
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The categories of this paper are: genomics
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The categories of this paper are: ecology
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The categories of this paper are: plant
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The categories of this paper are: cognition
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The categories of this paper are: bioengineering
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The categories of this paper are: toxicology
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The categories of this paper are: communication
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The categories of this paper are: microbiology
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The categories of this paper are: systems
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The categories of this paper are: bioinformatics
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The categories of this paper are: paleontology
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The categories of this paper are: biophysics