Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Label-retention expansion microscopy Expansion microscopy (ExM) increases the effective resolving power of any microscope by expanding the sample with swellable hydrogel. Since its invention, ExM has been successfully applied to a wide range of cell, tissue and animal samples. Still, fluorescence signal loss during polymerization and digestion limits molecular-scale imaging using ExM. Here we report the development of label-retention ExM (LR-ExM) with a set of trifunctional anchors that not only prevent signal loss but also enable high-efficiency labeling using SNAP and CLIP tags. We have demonstrated multicolor LR-ExM for a variety of subcellular structures. Combining LR-ExM with super-resolution Stochastic Optical Reconstruction Microscopy (STORM), we have achieved molecular resolution in the visualization of polyhedral lattice of clathrin-coated pits in situ.
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The categories of this paper are: molecular
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The categories of this paper are: scientific
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The categories of this paper are: pathology
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The categories of this paper are: behavior
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The categories of this paper are: epidemiology
2
The categories of this paper are: biology
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The categories of this paper are: systems
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The categories of this paper are: pharmacology
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The categories of this paper are: education
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The categories of this paper are: microbiology
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The categories of this paper are: biochemistry
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The categories of this paper are: cognition
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The categories of this paper are: physiology
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The categories of this paper are: immunology
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The categories of this paper are: bioinformatics
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The categories of this paper are: evolutionary