Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: An intein-split transactivator for intersectional neural imaging and optogenetic manipulation The complexity of brain circuitry is manifested by numerous cell types based on genetic marker, location and neural connectivity. Cell-type specific recording and manipulation is essential to disentangle causal neural mechanisms in physiology and behavior; however, many current approaches are largely limited by number of intersectional features, incompatibility of common effectors and insufficient gene expression. To tackle these limitations, we devise an intein-based intersectional synthesis of transactivator (IBIST) to selectively control gene expression of common effectors in specific cell types defined by a combination of multiple features. We validate the specificity and sufficiency of IBIST to control common effectors including fluorophores, optogenetic opsins and Ca2+ indicators in various intersectional conditions in vivo. Using IBIST-based Ca2+ imaging, we show that the IBIST can intersect up to five features, and that hippocampal cells tune differently to distinct emotional valences depending on the pattern of projection targets. Collectively, the IBIST multiplexes the capability to intersect cell-type features and is compatible with common effectors to effectively control gene expression, monitor and manipulate neural activities.
13
The categories of this paper are: animal
2
The categories of this paper are: biology
1
The categories of this paper are: bioinformatics
11
The categories of this paper are: cognition
25
The categories of this paper are: bioengineering
8
The categories of this paper are: genomics
28
The categories of this paper are: communication
27
The categories of this paper are: pathology
5
The categories of this paper are: cell
30
The categories of this paper are: scientific
23
The categories of this paper are: immunology
10
The categories of this paper are: and
22
The categories of this paper are: physiology
32
The categories of this paper are: zoology
26
The categories of this paper are: biochemistry
0
The categories of this paper are: genetics