Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Purifying selection enduringly acts on the sequence evolution of highly expressed proteins in Escherichia coli The evolutionary speed of a protein sequence is constrained by its expression level, with highly expressed proteins evolving relatively slowly. This negative correlation between expression levels and evolutionary rates (known as the E-R anticorrelation) has already been widely observed in past macroevolution between species from bacteria to animals. However, it remains unclear whether this seemingly general law also governs recent evolution, including past and de novo, within a species. However, the advent of genomic sequencing and high-throughput phenotyping, particularly for bacteria, has revealed fundamental gaps between the two evolutionary processes and has provided empirical data opposing the possible underlying mechanisms which are widely believed. These conflicts raise questions about the generalization of the E-R anticorrelation and the relevance of plausible mechanisms. To explore the ubiquitous impact of expression level on molecular evolution, and to test the relevance of the possible underlying mechanisms, we analyzed the genome sequences of 99 strains of Escherichia coli for microevolution in nature. We also analyzed genomic mutations accumulated under laboratory conditions as a model of de novo microevolution. Here, we show that the E-R anticorrelation is significant in both past and de novo microevolution in E. coli. Our data also confirmed ongoing purifying selection acting on highly expressed genes. Ongoing selection included codon-level purifying selection, supporting the relevance of the underlying mechanisms. However, their contributions to the constraints in recent evolution might be smaller than previously expected from past macroevolution.
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The categories of this paper are: molecular
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The categories of this paper are: bioengineering
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The categories of this paper are: immunology
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The categories of this paper are: animal
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The categories of this paper are: epidemiology
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The categories of this paper are: and
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The categories of this paper are: behavior
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The categories of this paper are: plant
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The categories of this paper are: microbiology
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The categories of this paper are: pharmacology
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The categories of this paper are: systems
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The categories of this paper are: developmental
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The categories of this paper are: communication
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The categories of this paper are: synthetic
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The categories of this paper are: education
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The categories of this paper are: pathology