Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: A framework for multiplex imaging optimization and reproducible analysis Multiplex imaging technologies are increasingly used for single-cell phenotyping and spatial characterization of tissues; however, transparent methods are needed for comparing the performance of platforms, protocols and analytical pipelines. We developed a python software, mplexable, for reproducible image processing and utilize Jupyter notebooks to share our optimization of signal removal, antibody specificity, background correction and batch normalization of the multiplex imaging with a focus on cyclic immunofluorescence (CyCIF). Our work both improves the CyCIF methodology and provides a framework for multiplexed image analytics that can be easily shared and reproduced.
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The categories of this paper are: zoology
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The categories of this paper are: pharmacology
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The categories of this paper are: plant
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The categories of this paper are: and
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The categories of this paper are: systems
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The categories of this paper are: pathology
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The categories of this paper are: genomics
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The categories of this paper are: toxicology
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The categories of this paper are: communication
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The categories of this paper are: physiology
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The categories of this paper are: bioengineering
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The categories of this paper are: neuroscience
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The categories of this paper are: microbiology
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The categories of this paper are: synthetic
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The categories of this paper are: biophysics
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The categories of this paper are: epidemiology