Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Computational inference, validation, and analysis of 5'UTR-leader sequences of alleles of immunoglobulin heavy chain variable genes Upstream and downstream sequences of immunoglobulin genes may affect the expression of such genes. However, these sequences are rarely studied or characterized in most studies of immunoglobulin repertoires. Inference from large, rearranged immunoglobulin transcriptome data sets offers an opportunity to define the upstream regions (5-untranslated regions and leader sequences). We have now established a new data pre-processing procedure to eliminate artifacts caused by a 5-RACE library generation process, reanalyzed a previously studied data set defining human immunoglobulin heavy chain genes, and identified novel upstream regions, as well as previously identified upstream regions that may have been identified in error. Upstream sequences were also identified for a set of previously uncharacterized germline gene alleles. Several novel upstream region variants were validated, for instance by their segregation to a single haplotype in heterozygotic subjects. SNPs representing several sequence variants were identified from population data. Finally, based on the outcomes of the analysis, we define a set of testable hypotheses with respect to the placement of particular alleles in complex IGHV locus haplotypes, and discuss the evolutionary relatedness of particular heavy chain variable genes based on sequences of their upstream regions.
5
The categories of this paper are: cell
13
The categories of this paper are: animal
30
The categories of this paper are: scientific
2
The categories of this paper are: biology
12
The categories of this paper are: behavior
28
The categories of this paper are: communication
25
The categories of this paper are: bioengineering
15
The categories of this paper are: developmental
21
The categories of this paper are: toxicology
31
The categories of this paper are: paleontology
3
The categories of this paper are: evolutionary
9
The categories of this paper are: synthetic
1
The categories of this paper are: bioinformatics
4
The categories of this paper are: neuroscience
0
The categories of this paper are: genetics
14
The categories of this paper are: biophysics