Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: AN IMAGING FLOW CYTOMETRY-BASED METHODOLOGY FOR THE ANALYSIS OF SINGLE EXTRACELLULAR VESICLES IN UNPROCESSED HUMAN PLASMA Extracellular vesicles (EVs) are tissue-specific particles released by cells containing valuable diagnostic information in the form of various biomolecules. To rule out selection bias or introduction of artefacts caused by EV isolation techniques, we present a clinically feasible, imaging flow cytometry (IFCM)-based methodology to phenotype and determine the concentration of EVs with a diameter [&le;]400 nm in human platelet-poor plasma (PPP) without prior isolation of EVs.

Instrument calibration (both size and fluorescence) were performed with commercial polystyrene beads. Detergent treatment of EVs was performed to discriminate true vesicular events from artefacts. Using a combination of markers (CFSE & Tetraspanins, or CD9 & CD31) we found that >90% of double-positive fluorescent events represented single EVs.

Through this work, we provide a framework that will allow the application of IFCM for EV analysis in peripheral blood plasma in a plethora of experimental and potentially diagnostic settings. Additionally, this direct approach for EV analysis will enable researchers to explore new corners of EV as cellular messengers in healthy and pathological conditions.
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The categories of this paper are: toxicology
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The categories of this paper are: genomics
23
The categories of this paper are: immunology
32
The categories of this paper are: zoology
31
The categories of this paper are: paleontology
24
The categories of this paper are: cancer
3
The categories of this paper are: evolutionary
19
The categories of this paper are: epidemiology
12
The categories of this paper are: behavior
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The categories of this paper are: systems
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The categories of this paper are: pharmacology
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The categories of this paper are: pathology
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The categories of this paper are: plant
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The categories of this paper are: neuroscience
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The categories of this paper are: biophysics
30
The categories of this paper are: scientific