Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Loss of Acta2 in cardiac fibroblasts does not affect myofibroblast differentiation or cardiac repair after myocardial infarction In response to myocardial infarction (MI), quiescent cardiac fibroblasts differentiate into myofibroblasts mediating tissue repair in the infarcted area. One of the most widely accepted markers of myofibroblast differentiation is the expression of Acta2 which encodes smooth muscle alpha-actin (SMA) that is assembled into stress fibers. However, the requirement of Acta2 in the myofibroblast differentiation of cardiac fibroblasts and its role in post-MI cardiac repair were still not known. To answer these questions, we generated a tamoxifen-inducible cardiac fibroblast-specific Acta2 knockout mouse line. Surprisingly, mice that lacked Acta2 in cardiac fibroblasts had a normal survival rate after MI. Moreover, Acta2 deletion did not affect the function or overall histology of infarcted hearts. No difference was detected in the proliferation, migration, or contractility between WT cardiac fibroblasts and Acta2-null cardiac myofibroblasts. Additional analysis identified that Acta2-null cardiac myofibroblasts had a normal total filamentous actin level and total actin level. Acta2 deletion caused a unique compensatory increase in the transcription level of Actg2 and a possible increase in the protein abundance of cytoplasmic actin isoforms. In conclusion, the deletion of Acta2 does not prevent the myofibroblast differentiation of cardiac fibroblasts or affect the post-MI cardiac repair, and the increase in the expression of non-SMA actin isoforms and the functional redundancy between actin isoforms are likely able to compensate for the loss of Acta2 in cardiac myofibroblasts.
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The categories of this paper are: cancer
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The categories of this paper are: biochemistry
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The categories of this paper are: zoology
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The categories of this paper are: plant
29
The categories of this paper are: education
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The categories of this paper are: genetics
31
The categories of this paper are: paleontology
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The categories of this paper are: biophysics
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The categories of this paper are: ecology
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The categories of this paper are: behavior
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The categories of this paper are: genomics
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The categories of this paper are: synthetic
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The categories of this paper are: bioengineering
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The categories of this paper are: epidemiology
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The categories of this paper are: scientific
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The categories of this paper are: evolutionary