Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Structural basis of RICs iron donation for iron-sulfur cluster biogenesis Escherichia coli YtfE is a di-iron protein, of the widespread RIC family, with capacity to donate iron, which is a crucial component of the biogenesis of the ubiquitous family of iron-sulfur proteins. Herein we identify in E. coli a previously unrecognized link between the YtfE protein and the major bacterial system for iron-sulfur cluster (ISC) assembly. We show that YtfE establishes protein-protein interactions with the scaffold IscU, where the transient cluster is formed, and the cysteine desulfurase IscS. Moreover, we found that promotion by YtfE of the formation of an Fe-S cluster in IscU requires two glutamates, E125 and E159 in YtfE. Both glutamates form part of the entrance of a protein channel in YtfE that links the di-iron centre to the surface. In particular, E125 is crucial for the exit of iron, as a single mutation to leucine closes the channel rendering YtfE inactive for the build-up of Fe-S clusters. Hence, we provide evidence for the key role of RICs as bacterial iron donor proteins involved in the biogenesis of Fe-S clusters.

ImportanceThe ubiquitous iron-sulfur proteins require specialized cellular machineries to promote the assembly of the cofactor. These systems include proteins that provide sulfur and iron, and scaffold proteins where the cluster is formed. Although largely studied the nature of the iron donor remains to be fully clarified. In this work, we show that Escherichia coli YtfE, which belongs to the RIC protein family, establishes protein-protein interactions with two of the major proteins of the ISC system, and we reveal the structural characteristics necessary for the exit of iron ions from YtfE. Altogether our results prove that RICs can be considered a family of iron donor proteins involved in the biogenesis of iron-sulfur containing proteins.
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The categories of this paper are: cell
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The categories of this paper are: microbiology
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The categories of this paper are: paleontology
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The categories of this paper are: developmental
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The categories of this paper are: synthetic
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The categories of this paper are: neuroscience
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The categories of this paper are: scientific
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The categories of this paper are: behavior
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The categories of this paper are: toxicology
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The categories of this paper are: bioinformatics
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The categories of this paper are: genomics
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The categories of this paper are: education
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The categories of this paper are: systems
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The categories of this paper are: communication
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The categories of this paper are: biology
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The categories of this paper are: animal