Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: The HMCES DNA-protein cross-link functions as a constitutive DNA repair intermediate The HMCES protein forms a covalent DNA-protein cross-link (DPC) with abasic (AP) sites in ssDNA, and the resulting HMCES-DPC is thought to suppress double-strand break formation in S phase. However, the dynamics of HMCES cross-linking and whether any DNA repair pathways normally include an HMCES-DPC intermediate remain unknown. Here, we show that an HMCES-DPC forms efficiently on the AP site generated during replication-coupled DNA interstrand cross-link (ICL) repair. We use this system to show that HMCES cross-links form on DNA after the replicative CMG helicase has passed over the AP site, and that HMCES is subsequently removed by the SPRTN protease. The HMCES-DPC suppresses DSB formation, slows translesion synthesis (TLS) past the AP site, and introduces a bias for insertion of deoxyguanosine opposite the AP site. These data show that HMCES-DPCs can form as constitutive intermediates in replication-coupled repair, and they suggest a general model of how HMCES protects AP sites during DNA replication.
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The categories of this paper are: cancer
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The categories of this paper are: behavior
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The categories of this paper are: animal
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The categories of this paper are: epidemiology
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The categories of this paper are: immunology
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The categories of this paper are: developmental
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The categories of this paper are: communication
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The categories of this paper are: education
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The categories of this paper are: pharmacology
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The categories of this paper are: synthetic
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The categories of this paper are: microbiology
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The categories of this paper are: pathology
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The categories of this paper are: physiology
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The categories of this paper are: bioengineering
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The categories of this paper are: paleontology
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The categories of this paper are: neuroscience