Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: High-Resolution Structure of the Nuclease Domain of the Human Parvovirus B19 Main Replication Protein NS1 Two new structures of the N-terminal domain of the main replication protein, NS1, of Human Parvovirus B19 (B19V) are presented. This domain (NS1-nuc) plays an important role in the "rolling hairpin" replication of the single-stranded B19V DNA genome, recognizing origin of replication sequences in double-stranded DNA, and cleaving (i.e. nicking) single-stranded DNA at a nearby site known as the trs. One structure of NS1-nuc is solved to 2.4 [A] and shows the positions of two bound phosphate ions. A second structure shows the position of a single divalent cation in the DNA nicking active site. The threedimensional structure of NS1-nuc is well conserved between the two forms, as well as with a previously solved structure of a sequence variant of the same domain, however shown here at significantly higher resolution. Using structures of NS1-nuc homologues bound to single- and double-stranded DNA, models for DNA recognition and nicking by B19V NS1-nuc are presented which predict residues important for DNA cleavage and for sequence specific recognition at the viral origin of replication.
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The categories of this paper are: neuroscience
30
The categories of this paper are: scientific
9
The categories of this paper are: synthetic
27
The categories of this paper are: pathology
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The categories of this paper are: plant
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The categories of this paper are: evolutionary
14
The categories of this paper are: biophysics
25
The categories of this paper are: bioengineering
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The categories of this paper are: cell
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The categories of this paper are: and
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The categories of this paper are: developmental
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The categories of this paper are: toxicology
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The categories of this paper are: education
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The categories of this paper are: cancer
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The categories of this paper are: cognition
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The categories of this paper are: genetics