Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Mcc1229, an Stx2a-amplifying microcin, is produced in vivo and requires CirA for activity Enterohemorrhagic E. coli (EHEC) strains, including the foodborne pathogen E. coli O157:H7, are responsible for thousands of hospitalizations each year. Various environmental triggers can modulate pathogenicity in EHEC by inducing expression of Shiga toxin (Stx), which is encoded on a lambdoid prophage and transcribed together with phage late genes. Cell-free supernatants of the sequence type (ST) 73 E. coli strain 0.1229 are potent inducers of Stx2a production in EHEC, suggesting that 0.1229 secretes a factor that activates the SOS response and leads to phage lysis. We previously demonstrated that this factor, designated microcin (Mcc) 1229, was proteinaceous and plasmid-encoded. To further characterize Mcc1229 and support its classification as a microcin, we investigated its regulation, determined its receptor, and identified loci providing immunity. Production of Mcc1229 was increased upon iron limitation, as determined by ELISA, lacZ fusions, and qRT-PCR. Spontaneous Mcc1229-resistant mutants and targeted gene deletion revealed that CirA was the Mcc1229 receptor. TonB, which interacts with CirA in the periplasm, was also essential for Mcc1229 import. Subcloning of the Mcc1229 plasmid indicated that Mcc activity was neutralized by two ORFs, each predicted to encode a domain of unknown function (DUF)-containing protein. In a germfree mouse model of infection, colonization with 0.1229 suppressed subsequent colonization of EHEC. Although Mcc1229 was produced in vivo, it was dispensable for colonization suppression. The regulation, import, and immunity determinants identified here are consistent with features of other Mccs, suggesting that Mcc1229 be included in this class of small molecules.
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The categories of this paper are: biochemistry
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The categories of this paper are: biology
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The categories of this paper are: genomics
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The categories of this paper are: animal
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The categories of this paper are: paleontology
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The categories of this paper are: scientific
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The categories of this paper are: toxicology
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The categories of this paper are: education
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The categories of this paper are: neuroscience
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The categories of this paper are: epidemiology
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The categories of this paper are: developmental
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The categories of this paper are: evolutionary
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The categories of this paper are: pathology
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The categories of this paper are: and
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The categories of this paper are: cognition
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The categories of this paper are: plant