Instruct: Identify the main and secondary category of Arxiv papers based on the titles and abstracts
Query: Compressive three-dimensional super-resolution microscopy with
  speckle-saturated fluorescence excitation   Nonlinear structured illumination microscopy (nSIM) is an effective approach
for super-resolution wide-field fluorescence microscopy with a theoretically
unlimited resolution. In nSIM, carefully designed, highly-contrasted
illumination patterns are combined with the saturation of an optical transition
to enable sub-diffraction imaging. While the technique proved useful for
two-dimensional imaging, extending it to three-dimensions (3D) is challenging
due to the fading/fatigue of organic fluorophores under intense cycling
conditions. Here, we present a compressed sensing approach that allows for the
first time 3D sub-diffraction nSIM of cultured cells by saturating fluorescence
excitation. Exploiting the natural orthogonality of transverse speckle
illumination planes, 3D probing of the sample is achieved by a single
two-dimensional scan. Fluorescence contrast under saturated excitation is
ensured by the inherent high density of intensity minima associated with
optical vortices in polarized speckle patterns. Compressed speckle microscopy
is thus a simple approach that enables 3D super-resolved nSIM imaging with
potentially considerably reduced acquisition time and photobleaching.les fast
3D super-resolved imaging with considerably minimized photo-bleaching.

33
The categories of this paper are: plasm-ph
9
The categories of this paper are: nlin
5
The categories of this paper are: gr-qc
34
The categories of this paper are: chem-ph
10
The categories of this paper are: q-bio
14
The categories of this paper are: math-ph
17
The categories of this paper are: q-fin
7
The categories of this paper are: hep-th
2
The categories of this paper are: math
24
The categories of this paper are: dg-ga
30
The categories of this paper are: q-alg
12
The categories of this paper are: nucl-th
26
The categories of this paper are: bayes-an
29
The categories of this paper are: funct-an
37
The categories of this paper are: supr-con
16
The categories of this paper are: stat