Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: HLA-G gene editing: a novel therapeutic alternative in cancer immunotherapy Cancer immunotherapies based mainly on the blockade of immune-checkpoint (IC) molecules by anti-IC antibodies offer new alternatives for treatment in oncological diseases. However, a considerable proportion of patients remain unresponsive to them. Hence, the development of novel clinical immunotherapeutic approaches and/or targets are crucial. In this context, targeting the immune-checkpoint HLA-G/ILT2/ILT4 has caused great interest since it is abnormally expressed in several malignancies generating a tolerogenic microenvironment. Here, we used CRISPR/Cas9 gene editing to block the HLA-G expression in two tumor cell lines expressing HLA-G, including a renal cell carcinoma (RCC7) and a choriocarcinoma (JEG-3). Different sgRNA/Cas9 plasmids targeting HLA-G exon 1 and 2 were transfected in both cell lines. Downregulation of HLA-G was reached to different degrees, including complete silencing. Most importantly, HLA-G - cells triggered a higher in vitro response of immune cells with respect to HLA-G + wild type cells. Altogether, we demonstrated for the first time the HLA-G downregulation through gene editing. We propose this approach as a first step to develop novel clinical immunotherapeutic approaches in cancer.
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The categories of this paper are: physiology
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The categories of this paper are: and
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The categories of this paper are: pharmacology
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The categories of this paper are: bioinformatics
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The categories of this paper are: zoology
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The categories of this paper are: paleontology
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The categories of this paper are: biophysics
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The categories of this paper are: genetics
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The categories of this paper are: communication
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The categories of this paper are: scientific
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The categories of this paper are: microbiology
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The categories of this paper are: immunology
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The categories of this paper are: molecular
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The categories of this paper are: genomics
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The categories of this paper are: pathology
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The categories of this paper are: systems