Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Modulation of PKM1/2 levels by steric blocking morpholinos alters the metabolic and pluripotent state of murine pluripotent stem cells Cellular metabolism plays both an active and passive role in embryonic development, pluripotency, and cell-fate decisions. However, little is known regarding the role of metabolism in regulating the recently described "formative" pluripotent state. The pluripotent developmental continuum features a metabolic switch from a bivalent metabolism (both glycolysis and oxidative phosphorylation) in naive cells, to predominantly glycolysis in primed cells. We investigated the role of pyruvate kinase muscle isoforms (PKM1/2) in naive, formative, and primed mouse embryonic stem cells through modulation of PKM1/2 mRNA transcripts using steric blocking morpholinos that downregulate PKM2 and upregulate PKM1. We have examined these effects in naive, formative, and primed cells by quantifying the effects of PKM1/2 modulation on pluripotent and metabolic transcripts and by measuring shifts in the population frequencies of cells expressing naive and primed cell surface markers by flow cytometry. Our results demonstrate that modulating PKM1 and PKM2 levels alters the transition from the naive state into a primed pluripotent state by enhancing the proportion of the affected cells seen in the "formative" state. Therefore, we conclude that PKM1/2 actively contributes to mechanisms that oversee early stem pluripotency and their progression towards a primed pluripotent state.
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The categories of this paper are: neuroscience
26
The categories of this paper are: biochemistry
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The categories of this paper are: behavior
1
The categories of this paper are: bioinformatics
31
The categories of this paper are: paleontology
29
The categories of this paper are: education
17
The categories of this paper are: molecular
27
The categories of this paper are: pathology
16
The categories of this paper are: microbiology
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The categories of this paper are: developmental
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The categories of this paper are: biophysics
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The categories of this paper are: evolutionary
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The categories of this paper are: ecology
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The categories of this paper are: genomics
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The categories of this paper are: bioengineering
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The categories of this paper are: toxicology