Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: The microtubule minus end-binding protein CAMSAP2 does not regulate microtubule dynamics in primary pancreatic β-cells but facilitates insulin secretion Glucose stimulation induces the remodeling of microtubules in Islet {beta}-cells to potentiate glucose-stimulated insulin secretion. CAMSAP2 is a microtubule minus-end binding protein and is reported to stabilize and position microtubules in several non-{beta}-cells, such as human retinal pigment epithelium cells. In immortalized insulinoma MIN6 cells, CAMSAP2 binds to and forms short stretches at microtubule minus ends in the cytoplasm, which is consistent with the reported subcellular localization and functions of CAMSAP2 in non-{beta}-cells. Surprisingly, we found that CAMSAP2 expressed in primary islet {beta}-cells does not form short stretches in the cytoplasm, but instead is localized to the Golgi apparatus. This novel localization is specific to {beta}-but not -cells in islets and it is independent of MT-binding. Knockdown of CAMSAP2 by shRNA impairs Golgi-ER trafficking, reduces total insulin content, and attenuates GSIS without affecting the MT dynamics or releasability of insulin granules in islet {beta}-cells. Corresponding to these results, we found that primary islets and MIN6 cells express different CAMSAP2 isoforms. We propose that primary islet {beta} cells use a novel CAMSAP2 isoform for a MT-independent non-canonical function, which is to promote Golgi-ER trafficking that supports efficient production of insulin secretory granules.
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The categories of this paper are: biophysics
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The categories of this paper are: microbiology
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The categories of this paper are: behavior
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The categories of this paper are: evolutionary
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The categories of this paper are: genomics
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The categories of this paper are: ecology
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The categories of this paper are: developmental
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The categories of this paper are: bioengineering
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The categories of this paper are: toxicology
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The categories of this paper are: and
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The categories of this paper are: animal
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The categories of this paper are: education
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The categories of this paper are: neuroscience
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The categories of this paper are: plant
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The categories of this paper are: bioinformatics
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The categories of this paper are: physiology