Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: The role of DDK and Treslin-MTBP in coordinating replication licensing and pre-Initiation Complex formation Treslin/Ticrr is required for the initiation of DNA replication and binds to MTBP (Mdm2 Binding Protein). Here we show that in Xenopus egg extract, MTBP forms an elongated tetramer with Treslin containing two molecules of each protein. Immunodepletion and add-back experiments show that Treslin-MTBP is rate-limiting for replication initiation. It is recruited onto chromatin before S phase starts and recruitment continues during S phase. We show that DDK activity both increases and strengthens the interaction of Treslin-MTBP with licensed chromatin. We also show that DDK activity cooperates with CDK activity to drive the interaction of Treslin-MTBP with TopBP1 which is a regulated crucial step in pre-Initiation Complex formation. These results suggest how DDK works together with CDKs to regulate Treslin-MTBP and plays a crucial in selecting which origins will undergo initiation.
10
The categories of this paper are: and
14
The categories of this paper are: biophysics
7
The categories of this paper are: ecology
22
The categories of this paper are: physiology
25
The categories of this paper are: bioengineering
28
The categories of this paper are: communication
1
The categories of this paper are: bioinformatics
20
The categories of this paper are: pharmacology
30
The categories of this paper are: scientific
8
The categories of this paper are: genomics
29
The categories of this paper are: education
21
The categories of this paper are: toxicology
4
The categories of this paper are: neuroscience
2
The categories of this paper are: biology
3
The categories of this paper are: evolutionary
31
The categories of this paper are: paleontology