Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Cryo-EM structures of the channelrhodopsin ChRmine in lipid nanodiscs Microbial channelrhodopsins are light-gated ion channels widely used for optogenetic manipulation of neuronal activity. ChRmine is a bacteriorhodopsin-like cation channelrhodopsin (BCCR) more closely related to ion pump rhodopsins than other channelrhodopsins. ChRmine displays unique properties favorable for optogenetics including high light sensitivity, a red-shifted activation spectrum, cation selectivity, and large photocurrents while its slow closing kinetics impede some applications. The structural basis for ChRmine function, or that of any other BCCR, is unknown. Here, we present cryo-EM structures of ChRmine in lipid nanodiscs in apo (opsin) and retinal-bound (rhodopsin) forms. The structures reveal an unprecedented trimeric architecture with a lipid filled central pore. Large electronegative cavities on either side of the membrane facilitate high conductance and selectivity for cations over protons. The retinal binding pocket structure suggests spectral and kinetic properties could be tuned with mutations and we identify ChRmine variants with two-fold increased and ten-fold decreased closing rates. These results provide insight into structural features that generate an ultra-potent microbial opsin and provide a platform for rational engineering of channelrhodopsins with improved properties that could expand the scale, depth, and precision of optogenetic manipulations.
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The categories of this paper are: evolutionary
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The categories of this paper are: zoology
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The categories of this paper are: communication
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The categories of this paper are: immunology
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The categories of this paper are: behavior
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The categories of this paper are: physiology
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The categories of this paper are: cognition
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The categories of this paper are: genomics
29
The categories of this paper are: education
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The categories of this paper are: paleontology
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The categories of this paper are: genetics
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The categories of this paper are: bioengineering
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The categories of this paper are: scientific
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The categories of this paper are: cancer
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The categories of this paper are: biophysics
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The categories of this paper are: pathology