Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Time-resolved assessment of single-cell protein secretion by sequencing Secreted proteins play critical roles in cellular communication and functional orchestration. Methods enabling concurrent measurement of cellular protein secretion, phenotypes and transcriptomes are still unavailable. Here, we describe time-resolved assessment of protein secretion from single cells by sequencing (TRAPS-seq). Released proteins are trapped onto cell surface via affinity matrices, and the captured analytes together with phenotypic markers can be probed by oligonucleotide-barcoded antibodies and simultaneously sequenced with transcriptomes. We used TRAPS-seq to interrogate secretion dynamics of pleiotropic cytokines (IFN-{gamma}, IL-2 and TNF-) of early activated human T lymphocytes, unraveling limited correlation between cytokine secretion and its transcript abundance with regard to timing and strength. We found that early central memory T cells with CD45RA expression (TCMRA) are the most effective responders in multiple cytokine secretion, and polyfunctionality involves unique yet dynamic combinations of gene signatures over time. TRAPS-seq presents a useful tool for cellular indexing of secretions, phenotypes, and transcriptomes at single-cell resolution.
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The categories of this paper are: systems
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The categories of this paper are: neuroscience
29
The categories of this paper are: education
20
The categories of this paper are: pharmacology
1
The categories of this paper are: bioinformatics
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The categories of this paper are: plant
14
The categories of this paper are: biophysics
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The categories of this paper are: microbiology
2
The categories of this paper are: biology
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The categories of this paper are: paleontology
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The categories of this paper are: animal
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The categories of this paper are: cognition
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The categories of this paper are: genetics
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The categories of this paper are: genomics
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The categories of this paper are: cell
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The categories of this paper are: pathology