Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Nanopore ReCappable Sequencing maps SARS-CoV-2 5' capping sites and provides new insights into the structure of sgRNAs The SARS-CoV-2 virus has a complex transcriptome characterised by multiple, nested sub genomic RNAs used to express structural and accessory proteins. Long-read sequencing technologies such as nanopore direct RNA sequencing can recover full-length transcripts, greatly simplifying the assembly of structurally complex RNAs. However, these techniques do not detect the 5' cap, thus preventing reliable identification and quantification of full-length, coding transcript models. Here we used Nanopore ReCappable Sequencing (NRCeq), a new technique that can identify capped full-length RNAs, to assemble a complete annotation of SARS-CoV-2 sgRNAs and annotate the location of capping sites across the viral genome. We obtained robust estimates of sgRNA expression across cell lines and viral isolates and identified novel canonical and non-canonical sgRNAs, including one that uses a previously un-annotated leader-to-body junction site. The data generated in this work constitute a useful resource for the scientific community and provide important insights into the mechanisms that regulate the transcription of SARS-CoV-2 sgRNAs.
7
The categories of this paper are: ecology
3
The categories of this paper are: evolutionary
28
The categories of this paper are: communication
18
The categories of this paper are: systems
12
The categories of this paper are: behavior
31
The categories of this paper are: paleontology
16
The categories of this paper are: microbiology
25
The categories of this paper are: bioengineering
14
The categories of this paper are: biophysics
2
The categories of this paper are: biology
10
The categories of this paper are: and
19
The categories of this paper are: epidemiology
9
The categories of this paper are: synthetic
21
The categories of this paper are: toxicology
24
The categories of this paper are: cancer
4
The categories of this paper are: neuroscience