Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Rickettsia typhi peptidoglycan mapping with data-dependent tandem mass spectrometry Rickettsia species are diverse Gram-negative obligate intracellular bacteria often pervasive in numerous invertebrates, as well as fungal, nematode and microeukaryotic hosts. Certain species are etiological agents for well-known arthropod-borne illnesses; e.g., R. rickettsii (Rocky Mountain Spotted Fever), R. prowazekii (Epidemic Typhus), and R. typhi, (Endemic Typhus). Living freely in eukaryotic cytosol presumably exposes rickettsiae to host cell immune receptors, particularly those recognizing bacterial cell envelope glycoconjugates. However, the mechanics of host recognition of rickettsiae remain poorly defined. As rickettsiae synthesize a canonical Gram-negative cell envelope that includes peptidoglycan (PGN) and lipopolysaccharide (LPS), structural insight on these macromolecules is important for deciphering host responses to these pathogens. In this work, PGN from R. typhi was digested and the resultant subunits were analyzed by two different, albeit complementary, sample preparation methods. Both approaches were subsequently subjected to liquid chromatography/mass spectrometry analysis to infer PGN structure. R. typhi PGN was determined to be similar to most other Gram-negative bacteria, with mDAP-type muropeptide subunits. However, additional alanine residues were observed elongating the muropeptide stems, rather than the glycine residues usually observed in Gram-negative bacterial PGN. Despite this deviation, R. typhi contains a murein layer that is predicted to agonize host cellular PGN receptors and be susceptible to PGN-targeting antimicrobials. This same structure is likely synthesized by all Rickettsia species, as bioinformatics and comparative genomics analyses indicate the biosynthesis of PGN is highly conserved. Determining how host cells process this canonical glycoconjugate during infection is crucial for identifying factors behind rickettsial pathogenesis, including immunoavoidance or proinflammatory mechanisms possibly employed by rickettsiae with varying pathogenic potential.
11
The categories of this paper are: cognition
31
The categories of this paper are: paleontology
27
The categories of this paper are: pathology
24
The categories of this paper are: cancer
15
The categories of this paper are: developmental
6
The categories of this paper are: plant
2
The categories of this paper are: biology
8
The categories of this paper are: genomics
10
The categories of this paper are: and
23
The categories of this paper are: immunology
22
The categories of this paper are: physiology
25
The categories of this paper are: bioengineering
1
The categories of this paper are: bioinformatics
4
The categories of this paper are: neuroscience
0
The categories of this paper are: genetics
12
The categories of this paper are: behavior