Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: An evaluation of resonant scanning as a high-speed imaging technique for two-photon imaging of cortical vasculature We demonstrate a simple, low-cost two-photon microscope design with both galvo-galvo and resonant-galvo scanning capabilities. We quantify and compare the signal-to-noise ratios and imaging speeds of the galvo-galvo and resonant-galvo scanning modes when used for murine neurovascular imaging. The two scanning modes perform as expected under shot-noise limited detection and are found to achieve comparable signal-to-noise ratios. Resonant-galvo scanning is capable of reaching desired signal-to-noise ratios using less acquisition time when higher excitation power can be used. Given equal excitation power and total pixel dwell time between the two methods, galvo-galvo scanning outperforms resonant-galvo scanning in image quality when detection deviates from being shot-noise limited.
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The categories of this paper are: microbiology
25
The categories of this paper are: bioengineering
6
The categories of this paper are: plant
31
The categories of this paper are: paleontology
14
The categories of this paper are: biophysics
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The categories of this paper are: developmental
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The categories of this paper are: biochemistry
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The categories of this paper are: genomics
13
The categories of this paper are: animal
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The categories of this paper are: epidemiology
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The categories of this paper are: communication
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The categories of this paper are: systems
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The categories of this paper are: pharmacology
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The categories of this paper are: biology
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The categories of this paper are: toxicology
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The categories of this paper are: cognition