Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Engraftment, fate, and function of HoxB8-conditional neutrophil progenitors in the unconditioned murine host The development and use of murine myeloid progenitor cell lines that are conditionally immortalized through expression of HoxB8 has provided a valuable tool for studies of neutrophil biology. Recent work has extended the utility of HoxB8-conditional progenitors to the in vivo setting via their transplantation into irradiated mice. Here, we describe the isolation of HoxB8-conditional progenitor cell lines that are unique in their ability to engraft in the naive host in the absence of conditioning of the hematopoietic niche. Our results indicate that HoxB8-conditional progenitors engraft in a {beta}1 integrin-dependent manner and transiently generate donor-derived mature neutrophils. Furthermore, we show that neutrophils derived in vivo from transplanted HoxB8-conditional progenitors are mobilized to the periphery and recruited to sites of inflammation in a manner that depends on the C-X-C chemokine receptor 2 and {beta}2 integrins, the same mechanisms that have been described for recruitment of endogenous primary neutrophils. Together, our studies advance the understanding of HoxB8-conditional neutrophil progenitors and describe an innovative tool that, by virtue of its ability to engraft in the naive host, will facilitate mechanistic in vivo experimentation on neutrophils.
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The categories of this paper are: communication
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The categories of this paper are: education
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The categories of this paper are: behavior
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The categories of this paper are: biochemistry
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The categories of this paper are: epidemiology
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The categories of this paper are: bioinformatics
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The categories of this paper are: ecology
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The categories of this paper are: biology
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The categories of this paper are: zoology
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The categories of this paper are: cognition
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The categories of this paper are: systems
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The categories of this paper are: genomics
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The categories of this paper are: pathology
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The categories of this paper are: evolutionary
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The categories of this paper are: molecular
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The categories of this paper are: developmental