Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: HEAT SHOCK FACTOR BINDING PROTEIN limits meiotic crossovers by repressing HEI10 transcription The number of meiotic crossovers is tightly controlled and most depend on pro-crossover ZMM proteins, such as the E3 ligase HEI10. Despite the importance of HEI10 dosage for crossover formation, how HEI10 transcription is controlled remains unexplored. In a forward genetic screen using a sensitive fluorescent seed crossover reporter in Arabidopsis thaliana we identify heat shock factor binding protein (HSBP) as a repressor of HEI10 transcription and crossover numbers. Using genome-wide crossover mapping and cytogenetics, we show that hsbp mutations or meiotic HSBP knockdowns increase ZMM-dependent crossovers towards the telomeres, mirroring the effects of HEI10 overexpression. Through RNA sequencing, DNA methylome and chromatin immunoprecipitation analysis, we reveal that HSBP directly represses HEI10 transcription by binding with heat shock factors (HSFs) at the HEI10 promoter and maintaining DNA methylation over the HEI10 5' untranslated region. Our findings provide insights into how the temperature response regulator HSBP restricts meiotic HEI10 transcription and crossover number by attenuating HSF activity.
28
The categories of this paper are: communication
24
The categories of this paper are: cancer
32
The categories of this paper are: zoology
26
The categories of this paper are: biochemistry
13
The categories of this paper are: animal
30
The categories of this paper are: scientific
29
The categories of this paper are: education
12
The categories of this paper are: behavior
15
The categories of this paper are: developmental
31
The categories of this paper are: paleontology
17
The categories of this paper are: molecular
16
The categories of this paper are: microbiology
3
The categories of this paper are: evolutionary
18
The categories of this paper are: systems
22
The categories of this paper are: physiology
14
The categories of this paper are: biophysics