Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Nuclear Export of Drosophila PERIOD contributes to temperature compensation of the circadian clock Circadian clocks are self-sustained molecular oscillators controlling daily changes of behavioral activity and physiology. For functional reliability and precision the frequency of these molecular oscillations must be stable at different environmental temperatures, known as  temperature compensation. Despite being an intrinsic property of all circadian clocks, this phenomenon is not well understood at the molecular level. Here we use behavioral and molecular approaches to characterize a novel mutation in the period (per) clock gene of Drosophila melanogaster, which alters a predicted nuclear export sequence (NES) of the PER protein. We show that this new perI530A allele leads to progressively longer behavioral periods and clock oscillations with increasing temperature in both clock neurons and peripheral clock cells. While the mutant PERI530A protein shows normal circadian fluctuations and post-translational modifications at cool temperatures, increasing temperatures lead to both, severe amplitude dampening and hypophosphorylation of PERI530A. We further show that PERI530A displays reduced repressor activity at warmer temperatures, presumably because it cannot inactivate the transcription factor CLOCK (CLK). With increasing temperatures nuclear accumulation of PERI530A within clock neurons is increased, suggesting that PER is normally exported out of the nucleus at warm temperatures. Consequently, downregulating the nuclear export factor CRM1 also leads to temperature-dependent changes of behavioral rhythms. In summary, our results suggest that the PER NES and the nuclear export of clock proteins play an important role in temperature compensation of the Drosophila circadian clock.
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The categories of this paper are: epidemiology
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The categories of this paper are: bioinformatics
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The categories of this paper are: toxicology
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The categories of this paper are: synthetic
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The categories of this paper are: paleontology
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The categories of this paper are: pharmacology
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The categories of this paper are: cancer
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The categories of this paper are: biology
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The categories of this paper are: cell
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The categories of this paper are: scientific
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The categories of this paper are: zoology
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The categories of this paper are: education
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The categories of this paper are: bioengineering
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The categories of this paper are: systems
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The categories of this paper are: plant
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The categories of this paper are: immunology