Instruct: Identify the main category of Medrxiv papers based on the titles and abstracts
Query: Limit of detection in different matrices of nineteen commercially available rapid antigen tests for the detection of SARS-CoV-2 In the context of the coronavirus disease 2019 (COVID-19) pandemic there has been an increase of the use of antigen-detection rapid diagnostic tests (Ag-RDT). The performance of Ag-RDT vary greatly between manufacturers and evaluating their analytical limit of detection (LOD) has become high priority. Here we describe a manufacturer-independent evaluation of the LOD of 19 marketed Ag-RDT using live SARS-CoV-2 spiked in different matrices: direct culture supernatant, a dry swab, and a swab in Amies. Additionally, the LOD using dry swab was investigated after 7 days storage at -80{degrees}C of the SARS-CoV-2 serial dilutions. An LOD of {approx} 5.0 x 102 pfu/ml (1.0 x 106 genome copies/ml) in culture media is defined as acceptable by the World Health Organization. Fourteen of nineteen Ag-RDTs (ActiveXpress, Espline, Excalibur, Innova, Joysbio, Mologic, NowCheck, Orient, PanBio, RespiStrip, Roche, Standard-F, Standard-Q and Sure-Status) exceeded this performance criteria using direct culture supernatant applied to the Ag-RDT. Six Ag-RDT were not compatible with Amies media and a decreased sensitivity of 2 to 20-fold was observed for eleven tests on the stored dilutions at -80{degrees}C for 7 days. Here, we provide analytical sensitivity data to guide appropriate test and sample type selection for use and for future Ag-RDT evaluations.
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The categories of this paper are: clinical
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The categories of this paper are: physical
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The categories of this paper are: systems
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The categories of this paper are: critical
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The categories of this paper are: orthopedics
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The categories of this paper are: hematology
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The categories of this paper are: immunology
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The categories of this paper are: respiratory
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The categories of this paper are: endocrinology
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The categories of this paper are: pediatrics
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The categories of this paper are: education
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The categories of this paper are: pharmacology
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The categories of this paper are: palliative
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The categories of this paper are: policy
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The categories of this paper are: occupational
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The categories of this paper are: oncology