Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Regulation of bacterial Type III Secretion System export gate opening Type III Secretion Systems (T3SS) transport proteins from the bacterial cytosol for assembly into cell surface nanomachines or for direct delivery into target eukaryotic cells. At the core of the flagellar T3SS, the FlhAB-FliPQR export gate regulates protein entry into the export channel whilst maintaining the integrity of the cell membrane. Here, we identify critical residues in the export gate FliR plug that stabilise the closed conformation, preserving the membrane permeability barrier, and we show that the gate opens and closes in response to export substrate availability. Our data indicate that FlhAB-FliPQR gate opening, which is triggered by substrate export signals, is energised by FlhA in a proton motive force-dependent manner. We present evidence that the export substrate and the FliJ stalk of the flagellar ATPase provide mechanistically distinct, non-redundant gate-activating signals that are critical for efficient export.
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The categories of this paper are: genetics
6
The categories of this paper are: plant
23
The categories of this paper are: immunology
13
The categories of this paper are: animal
25
The categories of this paper are: bioengineering
3
The categories of this paper are: evolutionary
29
The categories of this paper are: education
17
The categories of this paper are: molecular
9
The categories of this paper are: synthetic
4
The categories of this paper are: neuroscience
32
The categories of this paper are: zoology
2
The categories of this paper are: biology
14
The categories of this paper are: biophysics
5
The categories of this paper are: cell
22
The categories of this paper are: physiology
21
The categories of this paper are: toxicology