Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Isotropic Three-Dimensional Dual-Color Super-Resolution Microscopy with Metal-Induced Energy Transfer Over the last two decades, super-resolution microscopy has seen a tremendous development in speed and resolution, but for most of its methods, there exists a remarkable gap between lateral and axial resolution. Similar to conventional optical microscopy, the axial resolution is by a factor three to five worse than the lateral resolution. One recently developed method to close this gap is metal-induced energy transfer (MIET) imaging which achieves an axial resolution down to nanometers. It exploits the distance dependent quenching of fluorescence when a fluorescent molecule is brought close to a metal surface. In the present manuscript, we combine the extreme axial resolution of MIET imaging with the extraordinary lateral resolution of single-molecule localization microscopy, in particular with direct stochastic optical reconstruction microscopy (dSTORM). This combination allows us to achieve isotropic three-dimensional super-resolution imaging of sub-cellular structures. Moreover, we employed spectral demixing for implementing dualcolor MIET-dSTORM that allows us to image and co-localize, in three dimensions, two different cellular structures simultaneously.
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The categories of this paper are: biochemistry
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The categories of this paper are: genomics
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The categories of this paper are: physiology
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The categories of this paper are: toxicology
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The categories of this paper are: synthetic
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The categories of this paper are: systems
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The categories of this paper are: education
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The categories of this paper are: bioengineering
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The categories of this paper are: biology
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The categories of this paper are: pharmacology
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The categories of this paper are: immunology
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The categories of this paper are: and
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The categories of this paper are: genetics
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The categories of this paper are: cancer
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The categories of this paper are: epidemiology
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The categories of this paper are: pathology