Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Structural Basis for an Unprecedented Enzymatic Alkylation in Cylindrocyclophane Biosynthesis The cyanobacterial enzyme CylK assembles the cylindrocyclophane natural products by performing two unusual alkylation reactions, forming new carbon-carbon bonds between aromatic rings and secondary alkyl halide substrates. This transformation is unprecedented in biology and the structure and mechanism of CylK are unknown. Here, we report x-ray crystal structures of CylK, revealing a distinctive fusion of a Ca2+ binding domain and a {beta}-propeller fold. We use a mutagenic screening approach to locate CylKs active site at its domain interface, identifying two residues, Arg105 and Tyr473, that are required for catalysis. Anomalous diffraction datasets collected with bound bromide ions, a product analog, suggest these residues interact with the alkyl halide electrophile. Additional mutagenesis and molecular dynamics simulations implicates Asp440 and Glu374 in activating the nucleophilic aromatic ring. Bioinformatic analysis of CylK homologs from other cyanobacteria establishes that they conserve these key catalytic amino acids but they are likely associated with divergent reactivity and altered secondary metabolism. By gaining a molecular understanding of this unusual biosynthetic transformation, this work fills a gap in our understanding of how alkyl halides are activated and used by enzymes as biosynthetic intermediates, informing enzyme engineering, catalyst design, and natural product discovery.
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The categories of this paper are: bioengineering
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The categories of this paper are: cell
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The categories of this paper are: physiology
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The categories of this paper are: toxicology
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The categories of this paper are: pathology
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The categories of this paper are: animal
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The categories of this paper are: behavior
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The categories of this paper are: bioinformatics
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The categories of this paper are: communication
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The categories of this paper are: zoology
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The categories of this paper are: evolutionary
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The categories of this paper are: genetics
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The categories of this paper are: education
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The categories of this paper are: microbiology
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The categories of this paper are: and
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The categories of this paper are: ecology