Instruct: Identify the main and secondary category of Arxiv papers based on the titles and abstracts
Query: Soluble oligomerization provides a beneficial fitness effect on
  destabilizing mutations   Mutations create the genetic diversity on which selective pressures can act,
yet also create structural instability in proteins. How, then, is it possible
for organisms to ameliorate mutation-induced perturbations of protein stability
while maintaining biological fitness and gaining a selective advantage? Here we
used a new technique of site-specific chromosomal mutagenesis to introduce a
selected set of mostly destabilizing mutations into folA - an essential
chromosomal gene of E. coli encoding dihydrofolate reductase (DHFR) - to
determine how changes in protein stability, activity and abundance affect
fitness. In total, 27 E.coli strains carrying mutant DHFR were created. We
found no significant correlation between protein stability and its catalytic
activity nor between catalytic activity and fitness in a limited range of
variation of catalytic activity observed in mutants. The stability of these
mutants is strongly correlated with their intracellular abundance; suggesting
that protein homeostatic machinery plays an active role in maintaining
intracellular concentrations of proteins. Fitness also shows a significant
correlation with intracellular abundance of soluble DHFR in cells growing at
30oC. At 42oC, on the other hand, the picture was mixed, yet remarkable: a few
strains carrying mutant DHFR proteins aggregated rendering them nonviable, but,
intriguingly, the majority exhibited fitness higher than wild type. We found
that mutational destabilization of DHFR proteins in E. coli is counterbalanced
at 42oC by their soluble oligomerization, thereby restoring structural
stability and protecting against aggregation.

5
The categories of this paper are: gr-qc
34
The categories of this paper are: chem-ph
12
The categories of this paper are: nucl-th
21
The categories of this paper are: adap-org
16
The categories of this paper are: stat
24
The categories of this paper are: dg-ga
0
The categories of this paper are: hep-ph
26
The categories of this paper are: bayes-an
7
The categories of this paper are: hep-th
6
The categories of this paper are: astro-ph
22
The categories of this paper are: chao-dyn
8
The categories of this paper are: hep-ex
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The categories of this paper are: hep-lat
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The categories of this paper are: eess
14
The categories of this paper are: math-ph
33
The categories of this paper are: plasm-ph