Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: High-content single-cell combinatorial indexing Single-cell genomics assays have emerged as a dominant platform for interrogating complex biological systems. Methods to capture various properties at the single-cell level typically suffer a tradeoff between cell count and information content, which is defined by the number of unique and usable reads acquired per cell. We and others have described workflows that utilize single-cell combinatorial indexing (sci)1, leveraging transposase-based library construction2 to assess a variety of genomic properties in high throughput; however, these techniques often produce sparse coverage for the property of interest. Here, we describe a novel adaptor-switching strategy,  s3, capable of producing one-to-two order-of-magnitude improvements in usable reads obtained per cell for chromatin accessibility (s3-ATAC), whole genome sequencing (s3-WGS), and whole genome plus chromatin conformation (s3-GCC), while retaining the same high-throughput capabilities of predecessor  sci technologies. We apply s3 to produce high-coverage single-cell ATAC-seq profiles of mouse brain and human cortex tissue; and whole genome and chromatin contact maps for two low-passage patient-derived cell lines from a primary pancreatic tumor.
7
The categories of this paper are: ecology
19
The categories of this paper are: epidemiology
30
The categories of this paper are: scientific
32
The categories of this paper are: zoology
6
The categories of this paper are: plant
16
The categories of this paper are: microbiology
14
The categories of this paper are: biophysics
25
The categories of this paper are: bioengineering
11
The categories of this paper are: cognition
10
The categories of this paper are: and
31
The categories of this paper are: paleontology
28
The categories of this paper are: communication
8
The categories of this paper are: genomics
18
The categories of this paper are: systems
5
The categories of this paper are: cell
2
The categories of this paper are: biology