Instruct: Identify the main and secondary category of Arxiv papers based on the titles and abstracts
Query: A single step protein assay that is both detergent and reducer
  compatible: The cydex blue assay   Determination of protein concentration in often an absolute pre-requisite in
preparing samples for biochemical and proteomic analyses. However, current
protein assay methods are not compatible with both reducers and detergents,
which are however present simultaneously in most denaturing extraction buffers
used in proteomics and electrophoresis, and in particular in SDS
electrophoresis. We found that inclusion of cyclodextrins in a Coomassie
blue-based assay made it compatible with detergents, as cyclodextrins complex
detergents in a 1:1 molecular ratio. As this type of assay is intrinsically
resistant to reducers, we have thus developed a single step assay that is both
detergent and reducer compatible. Depending on the type and concentration of
detergents present in the sample buffer, either beta-cyclodextrin or
alpha-cyclodextrin can be used, the former being able to complex a wider range
of detergents and the latter being able to complex higher amounts of detergents
due to its greater solubility in water. Cyclodextrins are used at final
concentrations of 2-10 mg/mL in the assay mix. This typically allows to measure
samples containing as little as 0.1 mg/mL protein, in the presence of up to 2%
detergent and reducers such as 5 % mercaptoethanol or 50 mM DTT in a single
step with a simple spectrophotometric assay. This article is protected by
copyright. All rights reserved.

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The categories of this paper are: nlin
21
The categories of this paper are: adap-org
22
The categories of this paper are: chao-dyn
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The categories of this paper are: plasm-ph
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The categories of this paper are: supr-con
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The categories of this paper are: astro-ph
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The categories of this paper are: comp-gas
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The categories of this paper are: hep-ex
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The categories of this paper are: cond-mat
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The categories of this paper are: q-fin
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The categories of this paper are: hep-th
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The categories of this paper are: cmp-lg
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The categories of this paper are: nucl-th
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The categories of this paper are: gr-qc
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The categories of this paper are: hep-lat
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The categories of this paper are: solv-int