Instruct: Identify the main and secondary category of Arxiv papers based on the titles and abstracts
Query: A critical survey on the kinetic assays of DNA polymerase fidelity from
  a new theoretical perspective   The high fidelity of DNA polymerase is critical for the faithful replication
of genomic DNA. Several approaches were proposed to quantify the fidelity of
DNA polymerase. Direct measurements of the error frequency of the replication
products definitely give the true fidelity but turn out very hard to implement.
Two biochemical kinetic approaches, the steady-state assay and the
transient-state assay, were then suggested and widely adopted. In these assays,
the error frequency is indirectly estimated by using the steady-state or the
transient-state kinetic theory combined with the measured kinetic rates.
However, whether these indirectly estimated fidelities are equivalent to the
true fidelity has never been clarified theoretically, and in particular there
are different strategies to quantify the proofreading efficiency of DNAP but
often lead to inconsistent results. The reason for all these confusions is that
it's mathematically challenging to formulate a rigorous and general theory of
the true fidelity. Recently we have succeeded to establish such a theoretical
framework. In this paper, we develop this theory to make a comprehensive
examination on the theoretical foundation of the kinetic assays and the
relation between fidelities obtained by different methods. We conclude that
while the steady-state assay and the transient-state assay can always measure
the true fidelity of exonuclease-deficient DNA polymerases, they only do so for
exonuclease-efficient DNA polymerases conditionally (the proper way to use
these assays to quantify the proofreading efficiency is also suggested). We
thus propose a new kinetic approach, the single-molecule assay, which
indirectly but precisely characterizes the true fidelity of either
exonuclease-deficient or exonuclease-efficient DNA polymerases.

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The categories of this paper are: dg-ga
6
The categories of this paper are: astro-ph
26
The categories of this paper are: bayes-an
29
The categories of this paper are: funct-an
21
The categories of this paper are: adap-org
31
The categories of this paper are: ao-sci
1
The categories of this paper are: cs
13
The categories of this paper are: quant-ph
7
The categories of this paper are: hep-th
12
The categories of this paper are: nucl-th
4
The categories of this paper are: cond-mat
16
The categories of this paper are: stat
20
The categories of this paper are: acc-phys
14
The categories of this paper are: math-ph
30
The categories of this paper are: q-alg
0
The categories of this paper are: hep-ph