Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Single-molecule Mechanical Analysis of Strand Invasion in Human Telomere DNA Telomeres are essential chromosome end capping structures that safeguard the genome from dangerous DNA processing events. DNA strand invasion occurs during vital transactions at telomeres, including telomere length maintenance by the alternative lengthening of telomeres (ALT) pathway. During telomeric strand invasion, a single stranded guanine-rich (G-rich) DNA invades at a complimentary duplex telomere repeat sequence forming a displacement loop (D-loop) in which the displaced DNA consists of the same G-rich sequence as the invading single stranded DNA. Single stranded G-rich telomeric DNA readily folds into stable, compact, structures called G-quadruplexes (GQ) in vitro, and is anticipated to form within the context of a D-loop; however, evidence supporting this hypothesis is lacking. Here we report a magnetic tweezers assay that permits the controlled formation of telomeric D-loops (TDLs) within uninterrupted duplex human telomere DNA molecules of physiologically relevant lengths. Our results are consistent with a model wherein the displaced single stranded DNA of a TDL folds into a GQ. This study provides new insight into telomere structure and establishes a framework for development of novel therapeutics designed to target GQs at telomeres in cancer cells.
31
The categories of this paper are: paleontology
5
The categories of this paper are: cell
15
The categories of this paper are: developmental
1
The categories of this paper are: bioinformatics
20
The categories of this paper are: pharmacology
25
The categories of this paper are: bioengineering
27
The categories of this paper are: pathology
6
The categories of this paper are: plant
7
The categories of this paper are: ecology
32
The categories of this paper are: zoology
4
The categories of this paper are: neuroscience
2
The categories of this paper are: biology
9
The categories of this paper are: synthetic
12
The categories of this paper are: behavior
22
The categories of this paper are: physiology
10
The categories of this paper are: and