Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Viscoelastic Parameterization of Human Skin Cells to Characterize Material Behavior at Multiple Timescales Countless biophysical studies have sought distinct markers in the cellular mechanical response that could be linked to morphogenesis, homeostasis, and disease. Here, a novel iterative-fitting methodology is used to investigate the viscoelastic behavior at multiple relaxation times of human skin cells under physiologically relevant conditions. Past investigations often involved parameterizing linear elastic relationships and assuming purely Hertzian contact mechanics. However, linear elastic treatment fails to capture and properly account for the rich temporal information available in datasets. We demonstrate the performance superiority of the proposed iterative viscoelastic characterization method over standard open-search approaches. Our viscoelastic measurements revealed that 2D adherent metastatic melanoma cells exhibit reduced elasticity compared to normal counterparts--melanocytes and fibroblasts, whereas are significantly less viscous than only fibroblasts over timescales spanning three orders of magnitude. Interestingly, melanocytes are stiffer than melanoma cells, while being the less viscous cells measured. The measured loss angle indicates clear differential viscoelastic responses across multiple timescales between the measured cells. We propose the use of viscoelastic properties at multiple timescales as a mechanical biomarker of diseases. Altogether, this method provides new insight into the complex viscoelastic behavior of metastatic melanoma cells relevant to better understanding cancer metastasis aggression.
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The categories of this paper are: bioengineering
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The categories of this paper are: synthetic
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The categories of this paper are: developmental
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The categories of this paper are: paleontology
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The categories of this paper are: genomics
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The categories of this paper are: physiology
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The categories of this paper are: genetics
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The categories of this paper are: molecular
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The categories of this paper are: biochemistry
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The categories of this paper are: bioinformatics
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The categories of this paper are: toxicology
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The categories of this paper are: animal
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The categories of this paper are: cognition
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The categories of this paper are: education
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The categories of this paper are: systems
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The categories of this paper are: microbiology