Instruct: Identify the main and secondary category of Arxiv papers based on the titles and abstracts
Query: Assembly of Model Postsynaptic Densities Involves Interactions Auxiliary
  to Stoichiometric Binding   The assembly of functional biomolecular condensates often involves
liquid-liquid phase separation (LLPS) of proteins with multiple modular
domains, which can be folded or conformationally disordered to various degrees.
To understand the LLPS-driving domain-domain interactions, a fundamental
question is how readily the interactions in the condensed phase can be inferred
from inter-domain interactions in dilute solutions. In particular, are the
interactions leading to LLPS exclusively those underlying the formation of
discrete inter-domain complexes in homogeneous solutions? We address this
question by developing a mean-field LLPS theory of two stoichiometrically
constrained solute species. The theory is applied to the neuronal proteins
SynGAP and PSD-95, whose complex coacervate serves as a rudimentary model for
neuronal postsynaptic densities (PSDs). The predicted phase behaviors are
compared with experiments. Previously, a three-SynGAP, two-PSD-95 ratio was
determined for SynGAP/PSD-95 complexes in dilute solutions. However, when this
3:2 stoichiometry is uniformly imposed in our theory encompassing both dilute
and condensed phases, the tie-line pattern of the predicted SynGAP/PSD-95 phase
diagram differs drastically from that obtained experimentally. In contrast,
theories embodying alternate scenarios postulating auxiliary SynGAP-PSD-95 as
well as SynGAP-SynGAP and PSD-95-PSD-95 interactions in addition to those
responsible for stoichiometric SynGAP/PSD-95 complexes produce tie-line
patterns consistent with experiment. Hence, our combined
theoretical-experimental analysis indicates that weaker interactions or
higher-order complexes beyond the 3:2 stoichiometry, but not yet documented,
are involved in the formation of SynGAP/PSD-95 condensates, imploring future
efforts to ascertain the nature of these auxiliary interactions in PSD-like
LLPS.

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The categories of this paper are: econ
22
The categories of this paper are: chao-dyn
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The categories of this paper are: adap-org
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The categories of this paper are: q-alg
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The categories of this paper are: chem-ph
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The categories of this paper are: astro-ph
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The categories of this paper are: physics
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The categories of this paper are: hep-th
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The categories of this paper are: nucl-th
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The categories of this paper are: stat
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The categories of this paper are: cs
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The categories of this paper are: bayes-an
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The categories of this paper are: hep-lat
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The categories of this paper are: hep-ph
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The categories of this paper are: quant-ph
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The categories of this paper are: supr-con