Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: DNA Origami Voltage Sensors for Transmembrane Potentials with Single-Molecule Sensitivity Signal transmission in neurons goes along with changes in the transmembrane potential. To report them, different approaches including optical voltage-sensing dyes and genetically encoded voltage indicators have evolved. Here, we present a DNA nanotechnology-based system. Using DNA origami, we incorporate and optimize different properties such as membrane targeting and voltage sensing modularly. As a sensing unit, we use a hydrophobic red dye anchored to the membrane and an anionic green dye at the DNA connecting the DNA origami and the membrane dye anchor. Voltage-induced displacement of the anionic donor unit is read out by changes of Fluorescence Resonance Energy Transfer (FRET) of single sensors attached to liposomes. They show a FRET change of [~]5% for {Delta}{Psi}=100 mV and allow adapting the potential range of highest sensitivity. Further, the working mechanism is rationalized by molecular dynamics simulations. Our approach holds potential for the application as non-genetically encoded sensors at membranes.
21
The categories of this paper are: toxicology
10
The categories of this paper are: and
20
The categories of this paper are: pharmacology
16
The categories of this paper are: microbiology
6
The categories of this paper are: plant
32
The categories of this paper are: zoology
18
The categories of this paper are: systems
25
The categories of this paper are: bioengineering
0
The categories of this paper are: genetics
3
The categories of this paper are: evolutionary
1
The categories of this paper are: bioinformatics
28
The categories of this paper are: communication
19
The categories of this paper are: epidemiology
31
The categories of this paper are: paleontology
26
The categories of this paper are: biochemistry
13
The categories of this paper are: animal