Instruct: Identify the main and secondary category of Arxiv papers based on the titles and abstracts
Query: Rapid focus map surveying for whole slide imaging with continues sample
  motion   Whole slide imaging (WSI) has recently been cleared for primary diagnosis in
the US. A critical challenge of WSI is to perform accurate focusing in high
speed. Traditional systems create a focus map prior to scanning. For each focus
point on the map, sample needs to be static in the x-y plane and axial scanning
is needed to maximize the contrast. Here we report a novel focus map surveying
method for WSI. The reported method requires no axial scanning, no additional
camera and lens, works for stained and transparent samples, and allows
continuous sample motion in the surveying process. It can be used for both
brightfield and fluorescence WSI. By using a 20X, 0.75 NA objective lens, we
demonstrate a mean focusing error of ~0.08 microns in the static mode and ~0.17
microns in the continuous motion mode. The reported method may provide a
turnkey solution for most existing WSI systems for its simplicity, robustness,
accuracy, and high-speed. It may also standardize the imaging performance of
WSI systems for digital pathology and find other applications in high-content
microscopy such as DNA sequencing and time-lapse live-cell imaging.

12
The categories of this paper are: nucl-th
15
The categories of this paper are: nucl-ex
35
The categories of this paper are: mtrl-th
30
The categories of this paper are: q-alg
6
The categories of this paper are: astro-ph
10
The categories of this paper are: q-bio
23
The categories of this paper are: solv-int
26
The categories of this paper are: bayes-an
5
The categories of this paper are: gr-qc
27
The categories of this paper are: comp-gas
3
The categories of this paper are: physics
32
The categories of this paper are: atom-ph
34
The categories of this paper are: chem-ph
13
The categories of this paper are: quant-ph
17
The categories of this paper are: q-fin
31
The categories of this paper are: ao-sci