Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Sen1 is a master regulator of transcription-driven conflicts Cellular homeostasis requires the coordination of several machineries concurrently engaged on the DNA. Wide-spread transcription can interfere with other processes and transcription-replication conflicts (TRCs) threaten genome stability. The conserved Sen1 helicase terminates non-coding transcription, but also interacts with the replisome and reportedly resolves genotoxic R-loops. Sen1 prevents genomic instability but how this relates to its molecular functions remains unclear. We generated high-resolution, genome-wide maps of transcription-dependent conflicts and R-loops using a Sen1 mutant that has lost interaction with the replisome but is termination proficient. We show that Sen1 removes RNA polymerase II at TRCs within genes and the rDNA, but also at sites of transcription-transcription conflicts under physiological conditions, thus qualifying as a "master regulator of conflicts". We demonstrate that genomic stability is only affected by Sen1 mutation when, in addition to its role at the replisome, termination of non-coding transcription or R-loop removal are additionally compromised.
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The categories of this paper are: systems
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The categories of this paper are: immunology
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The categories of this paper are: cognition
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The categories of this paper are: synthetic
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The categories of this paper are: education
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The categories of this paper are: bioinformatics
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The categories of this paper are: genetics
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The categories of this paper are: bioengineering
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The categories of this paper are: physiology
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The categories of this paper are: biochemistry
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The categories of this paper are: cancer
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The categories of this paper are: developmental
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The categories of this paper are: pathology
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The categories of this paper are: cell
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The categories of this paper are: molecular
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The categories of this paper are: and