Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Lnc956-TRIM28-HSP90B1 complex on replication forks promotes CMG helicase retention to ensure stem cell genomic stability and embryogenesis Replication stress is a major source of endogenous DNA damage. Despite that numerous proteins have been identified on replication forks to modulate fork or replication machinery activities, it remains unexplored whether non-coding RNAs can localize on stalled forks and play critical regulatory roles. Here we identify an uncharacterized lncRNA NONMMUT028956 (Lnc956 for short) predominantly expressed in mouse embryonic stem cells. Lnc956 is recruited to stalled replication forks to prevent fork collapse and preserve genomic stability, and is essential for mouse embryogenesis. Mechanistically, it drives assembly of the Lnc956-TRIM28-HSP90B1 ribonucleoprotein (RNP) complex on stalled forks in an inter-dependent manner downstream of ATR signaling. This RNP complex physically associates with MCM2-7 hexamer via TRIM28 and directly regulates the CMG helicase retention on chromatin. The regulation of RNP on CMG retention is mediated by HSP90B1s chaperoning function. These findings reveal a novel pathway which actively regulates replisome retention to prevent fork collapse.
27
The categories of this paper are: pathology
0
The categories of this paper are: genetics
3
The categories of this paper are: evolutionary
6
The categories of this paper are: plant
23
The categories of this paper are: immunology
22
The categories of this paper are: physiology
26
The categories of this paper are: biochemistry
25
The categories of this paper are: bioengineering
31
The categories of this paper are: paleontology
17
The categories of this paper are: molecular
30
The categories of this paper are: scientific
21
The categories of this paper are: toxicology
24
The categories of this paper are: cancer
12
The categories of this paper are: behavior
1
The categories of this paper are: bioinformatics
32
The categories of this paper are: zoology