Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Emerging SARS-CoV-2 variants reduce neutralization sensitivity to convalescent sera and monoclonal antibodies SARS-CoV-2 Spike-specific antibodies contribute the majority of the neutralizing activity in most convalescent human sera. Two SARS-CoV-2 variants, N501Y.V1 (also known as B.1.1.7 lineage or VOC-202012/01) and N501Y.V2 (B.1.351 lineage), reported from the United Kingdom and South Africa, contain several mutations in the receptor binding domain of Spike and are of particular concern. To address the infectivity and neutralization escape phenotypes potentially caused by these mutations, we used SARS-CoV-2 pseudovirus system to compare the viral infectivity, as well as the neutralization activities of convalescent sera and monoclonal antibodies (mAbs) against SARS-CoV-2 variants. Our results showed that N501Y Variant 1 and Variant 2 increase viral infectivity compared to the reference strain (wild-type, WT) in vitro. At 8 months after symptom onset, 17 serum samples of 20 participants (85%) retaining titers of ID50 >40 against WT pseudovirus, whereas the NAb titers of 8 samples (40%) and 18 samples (90%) decreased below the threshold against N501Y.V1 and N501Y.V2, respectively. In addition, both N501Y Variant 1 and Variant 2 reduced neutralization sensitivity to most (6/8) mAbs tested, while N501Y.V2 even abrogated neutralizing activity of two mAbs. Taken together the results suggest that N501Y.V1 and N501Y.V2 reduce neutralization sensitivity to some convalescent sera and mAbs.
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The categories of this paper are: immunology
1
The categories of this paper are: bioinformatics
27
The categories of this paper are: pathology
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The categories of this paper are: toxicology
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The categories of this paper are: molecular
26
The categories of this paper are: biochemistry
24
The categories of this paper are: cancer
15
The categories of this paper are: developmental
22
The categories of this paper are: physiology
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The categories of this paper are: neuroscience
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The categories of this paper are: and
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The categories of this paper are: genomics
31
The categories of this paper are: paleontology
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The categories of this paper are: scientific
2
The categories of this paper are: biology
0
The categories of this paper are: genetics