Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Native MOWChIP-seq: Genome-wide profiles of key protein bindings reveal functional differences among various brain regions Genome-wide profiling of interactions between genome and various functional proteins is critical for understanding regulatory processes involved in development and diseases. Conventional assays require a large number of cells and high-quality data on tissue samples are scarce. Here we optimized a low-input chromatin immunoprecipitation followed by sequencing (ChIP-seq) technology for profiling RNA polymerase II (Pol II), transcription factor (TF), and enzyme binding at the genome scale. The new approach, termed native MOWChIP-seq, produces high-quality binding profiles using 1000-50,000 cells. We used the approach to examine the binding of Pol II and two TFs (EGR1 and MEF2C) in cerebellum and prefrontal cortex of mouse brain and found that their binding profiles are highly reflective of the functional differences between the two brain regions. Our analysis reveals the potential for linking genome-wide TF or Pol II profiles with neuroanatomical origins of brain cells.
16
The categories of this paper are: microbiology
26
The categories of this paper are: biochemistry
29
The categories of this paper are: education
3
The categories of this paper are: evolutionary
5
The categories of this paper are: cell
6
The categories of this paper are: plant
32
The categories of this paper are: zoology
17
The categories of this paper are: molecular
18
The categories of this paper are: systems
14
The categories of this paper are: biophysics
9
The categories of this paper are: synthetic
10
The categories of this paper are: and
28
The categories of this paper are: communication
12
The categories of this paper are: behavior
19
The categories of this paper are: epidemiology
20
The categories of this paper are: pharmacology