Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Ataxin-2, Twenty-four and Dicer-2 are components of a non-canonical cytoplasmic polyadenylation complex Cytoplasmic polyadenylation is a mechanism to promote mRNA translation in a wide variety of biological contexts. A canonical complex centered around the conserved RNA-binding protein family CPEB has been shown to be responsible for this process. We have previously reported evidence for an alternative non-canonical, CPEB-independent complex in Drosophila, of which the RNA-interference factor Dicer-2 is a component. Here, we investigate Dicer-2 mRNA targets and protein co-factors in cytoplasmic polyadenylation. Using RIP-Seq analysis we identify hundreds of novel Dicer-2 target transcripts, [~]50% of which were previously found as targets of the cytoplasmic poly(A) polymerase Wispy, suggesting widespread roles of Dicer-2 in cytoplasmic polyadenylation. Large-scale immunoprecipitation revealed Ataxin-2 and Twenty-four among the high-confidence interactors of Dicer-2. Functional analysis indicate that both factors form an RNA-independent complex with Dicer-2, and are required for cytoplasmic polyadenylation of Dicer-2 targets. Our results reveal the composition of a novel cytoplasmic polyadenylation complex that operates during Drosophila early embryogenesis.
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The categories of this paper are: cancer
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The categories of this paper are: toxicology
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The categories of this paper are: plant
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The categories of this paper are: biophysics
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The categories of this paper are: cell
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The categories of this paper are: developmental
31
The categories of this paper are: paleontology
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The categories of this paper are: systems
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The categories of this paper are: microbiology
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The categories of this paper are: bioengineering
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The categories of this paper are: pharmacology
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The categories of this paper are: cognition
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The categories of this paper are: genetics
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The categories of this paper are: evolutionary
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The categories of this paper are: communication
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The categories of this paper are: epidemiology