Instruct: Identify the main and secondary category of Arxiv papers based on the titles and abstracts
Query: CsrA and its regulators control the time-point of ColicinE2 release in
  Escherichia coli   The bacterial SOS response is a cellular reaction to DNA damage, that, among
other actions, triggers the expression of colicin - toxic bacteriocins in
Escherichia coli that are released to kill close relatives competing for
resources. However, it is largely unknown, how the complex network regulating
toxin expression controls the time-point of toxin release to prevent premature
release of inefficient protein concentrations. Here, we study how different
regulatory mechanisms affect production and release of the bacteriocin
ColicinE2 in Escherichia coli. Combining experimental and theoretical
approaches, we demonstrate that the global carbon storage regulator CsrA
controls the duration of the delay between toxin production and release and
emphasize the importance of CsrA sequestering elements for the timing of
ColicinE2 release. In particular, we show that ssDNA originating from
rolling-circle replication of the toxin-producing plasmid represents a yet
unknown additional CsrA sequestering element, which is essential in the
ColicinE2-producing strain to enable toxin release by reducing the amount of
free CsrA molecules in the bacterial cell. Taken together, our findings show
that CsrA times ColicinE2 release and reveal a dual function for CsrA as an
ssDNA and mRNA-binding protein, introducing ssDNA as an important
post-transcriptional gene regulatory element.

27
The categories of this paper are: comp-gas
12
The categories of this paper are: nucl-th
0
The categories of this paper are: hep-ph
13
The categories of this paper are: quant-ph
9
The categories of this paper are: nlin
32
The categories of this paper are: atom-ph
21
The categories of this paper are: adap-org
6
The categories of this paper are: astro-ph
30
The categories of this paper are: q-alg
17
The categories of this paper are: q-fin
1
The categories of this paper are: cs
25
The categories of this paper are: patt-sol
15
The categories of this paper are: nucl-ex
2
The categories of this paper are: math
23
The categories of this paper are: solv-int
5
The categories of this paper are: gr-qc