Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: RNase H1 exists as phase-separated assemblies in association with elongating RNA Polymerase II during transcription R-loops are three stranded nucleic acid structures consisting of a RNA/DNA hybrid and a single stranded displaced DNA. RNase H1 is an endonuclease which specifically degrades the RNA moiety in RNA-DNA hybrids. Here, we report that RNase H1 interacts with transcription elongation machinery during active transcription in a liquid-liquid phase separation (LLPS) dependent manner. We show that RNase H1 interacts with nascent RNA, and transcription elongation machinery in Hela cells, using in-situ nascent RNA labelling and Proximity ligation assay. Further, RNase H1 was found to exhibit properties of liquid-like condensates both in vitro and in vivo. Interestingly, RNase H1 interaction with elongating RNA Polymerase II can be disrupted by chemicals that perturb LLPS. Importantly, we show that LLPS is important for regulation of R-loop levels in the cell. Based on our results, we propose that RNase H1 exists as phase-separated assemblies in association with elongating RNA Polymerase II during active transcription.
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The categories of this paper are: genetics
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The categories of this paper are: genomics
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The categories of this paper are: zoology
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The categories of this paper are: synthetic
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The categories of this paper are: neuroscience
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The categories of this paper are: ecology
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The categories of this paper are: cognition
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The categories of this paper are: cancer
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The categories of this paper are: evolutionary
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The categories of this paper are: systems
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The categories of this paper are: molecular
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The categories of this paper are: microbiology
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The categories of this paper are: communication
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The categories of this paper are: education
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The categories of this paper are: animal
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The categories of this paper are: behavior