Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Analysis of calcium dynamics for dim-light responses in rod and cone photoreceptors Rod and cone photoreceptors in vertebrate eyes are fundamental sensory neurons underlying vision. They use a sophisticated signal transduction pathway consisting of a series of biochemical processes to convert the absorption of light into an electrical response. Several of these processes are modulated by feedback mechanisms that depend on the intracellular Ca2+ concentration. In this work we use a representative phototransduction model with fast buffering to study how the Ca2+ dynamics affects the light response of mouse rod and cone photoreceptors. We obtain analytic solutions for dim-light stimulation that reveal how the dynamics of the Ca2+ concentration and the electrical current are correlated. We show how modifying the Ca2+ dynamics via buffering affects amplitude and waveform of flash and step responses. We infer that under normal physiological conditions the change in the Ca2+ concentration is proportional to the current. We show that damped oscillations appear if the Ca2+ dynamics becomes slowed down and delayed with respect to the current, e.g. through the application of buffers. We find that oscillations do not require the presence of slow buffers. We show that the phase space of the dim-light response is controlled by the ratio between the effective rate ca that controls the Ca2+ dynamics, and the dark turnover rate of cyclic GMP {beta}d. Finally, we investigate how the light response is affected by modifying the extracellular Ca2+ concentration. In summary, we provide a comprehensive analysis that reveals how the Ca2+ dynamics affects the dim-light response in rods and cones.

SignificanceThe signal transduction pathway of vertebrate rod and cone photoreceptors transforms light into an electrical response. It is regulated by several feedback processes that depend on the intracellular Ca2+ concentration. Despite of the importance of this feedback for the photoreceptor functioning, it is not well studied how modifying the Ca2+ dynamics affects the light response, for example by adding buffers. Here we derive analytic results for dim-light stimulations that provide insight into how the Ca2+ dynamics and the electrical current response are correlated. Our analysis can be used to infer properties of the Ca2+ dynamics from the observed current response, and provides insight if direct measurements of the Ca2+ dynamics in the small outer segment of a photoreceptors are not available.
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The categories of this paper are: bioengineering
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The categories of this paper are: plant
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The categories of this paper are: cancer
8
The categories of this paper are: genomics
1
The categories of this paper are: bioinformatics
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The categories of this paper are: systems
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The categories of this paper are: pharmacology
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The categories of this paper are: ecology
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The categories of this paper are: biology
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The categories of this paper are: toxicology
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The categories of this paper are: scientific
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The categories of this paper are: synthetic
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The categories of this paper are: microbiology
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The categories of this paper are: developmental
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The categories of this paper are: cell
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The categories of this paper are: molecular