Instruct: Identify the main category of Medrxiv papers based on the titles and abstracts
Query: Clonal diversity determines persistence of SARS-CoV-2 epitope-specific T cell response T cells play a pivotal role in reducing disease severity during SARS-CoV-2 infection and formation of long-term immune memory. We studied 50 COVID-19 convalescent patients and found that T cell response was induced more frequently and persisted longer than circulating antibodies. To identify epitopes that give rise to long-lived T cell memory, we performed ex vivo T cell expansion, MHC-tetramer cell-sorting, and high-throughput sequencing. We identified 756 clonotypes specific to nine known CD8+ T cell receptor (TCR) epitopes. Some epitopes were recognized by highly similar public clonotypes with restricted variable and joining segment usage. Receptors for other epitopes were extremely diverse, suggesting alternative modes of recognition. We also tracked persistence of epitope-specific response and individual clonotypes for a median of eight months after infection. The number of recognized epitopes per patient and quantity of epitope-specific clonotypes decreased over time, but the studied epitopes were characterized by uneven decline in the number of specific T cells. Epitopes with more clonally diverse TCR repertoires induced more pronounced and durable responses. In contrast, the abundance of specific clonotypes in peripheral circulation had no influence on their persistence. Our study demonstrates the durability of SARS-CoV-2-specific CD8+ memory, and offers important implications for vaccine design.
31
The categories of this paper are: imaging
13
The categories of this paper are: genetic
8
The categories of this paper are: epidemiology
29
The categories of this paper are: anesthesia
48
The categories of this paper are: critical
5
The categories of this paper are: health
0
The categories of this paper are: neurology
50
The categories of this paper are: medical
46
The categories of this paper are: therapeutics
18
The categories of this paper are: global
71
The categories of this paper are: ethics
32
The categories of this paper are: rehabilitation
3
The categories of this paper are: sexual
57
The categories of this paper are: primary
36
The categories of this paper are: economics
9
The categories of this paper are: diseases