Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Targeted Neuronal Activation of the Gastrointestinal Tract Shapes the Environment of the Gut in Mice The enteric nervous system (ENS) coordinates responses that shape the intestinal milieu, and participates in bi-directional communication with various organs including the brain. Tools to study, and knowledge of, ENS development and function lag behind brain research. Herein, we deploy recombinant adeno-associated viral (rAAV) vectors with enhanced tropism for the gut to map and activate gut associated neurons in mice with spatial and temporal resolution. rAAV-mediated fluorescent labelling coupled with whole-tissue clearing methods in the small intestine and colon reveal novel neuronal architectures in the gut of mice. Viral delivery of genetically-encoded calcium indicators enable in vivo measurement of ENS activity. Further, we employed chemogentics to specifically activate gut neurons that express choline acetyltransferase (ChAT+) or tyrosine hydroxylase (TH+). Targeted activation of ChAT+ or TH+ neuronal populations associated with the gastrointestinal (GI) tract alters the intestinal transcriptome, mouse and microbial proteomes, metagenome, and fecal metabolome. We reveal previously unknown and broad roles for enteric neurons in modulating GI physiology, mucosal immunity, and microbiome structure, and provide evidence for novel functions by the ENS such as regulating fungal colonization and shaping of bile acid profiles in the gut. These advanced experimental tools and rich datasets with multi-parameter characterization of the gut ecosystem may facilitate expanded understanding and study of the ENS.
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The categories of this paper are: systems
26
The categories of this paper are: biochemistry
5
The categories of this paper are: cell
29
The categories of this paper are: education
15
The categories of this paper are: developmental
4
The categories of this paper are: neuroscience
7
The categories of this paper are: ecology
0
The categories of this paper are: genetics
19
The categories of this paper are: epidemiology
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The categories of this paper are: pharmacology
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The categories of this paper are: behavior
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The categories of this paper are: cancer
2
The categories of this paper are: biology
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The categories of this paper are: microbiology
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The categories of this paper are: genomics
32
The categories of this paper are: zoology