Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Role of glutathione on cell adhesion and volume. Glutathione is the most abundant thiol in animal cells. Reduced glutathione (GSH) is a major intracellular antioxidant neutralizing free radicals and detoxifying electrophiles. It plays important roles in many cellular processes, including cell differentiation, proliferation, and apoptosis. In the present study we demonstrate that extracellular concentration of reduced glutathione markedly increases cell volume within few hours, in a dose-response manner. Pre-incubation of cells with BSO, the inhibitor of {gamma}-glutamylcysteine synthetase, responsible for the first step in intracellular glutathione synthesis did not change the effect of reduced glutathione on cell volume suggesting a mechanism limited to the interaction of extracellular reduced glutathione on cell membrane. Similarly, inhibition of {gamma}-glutamylcyclotransferase involved in intracellular glutamate production had no effect on the action of reduced glutathione. Oxidized glutathione exerted no effect on cell volume. Results show that reduced GSH decreases cell adhesion resulting in an increased cell volume. Since many cell types are able to export GSH, the present results suggest that this could be a fundamental self-regulation of cell volume, giving the cells a self-control on their adhesion proteins.
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The categories of this paper are: neuroscience
23
The categories of this paper are: immunology
10
The categories of this paper are: and
25
The categories of this paper are: bioengineering
21
The categories of this paper are: toxicology
24
The categories of this paper are: cancer
6
The categories of this paper are: plant
11
The categories of this paper are: cognition
19
The categories of this paper are: epidemiology
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The categories of this paper are: evolutionary
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The categories of this paper are: developmental
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The categories of this paper are: microbiology
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The categories of this paper are: genomics
29
The categories of this paper are: education
0
The categories of this paper are: genetics
31
The categories of this paper are: paleontology