Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: A platform for efficient establishment, expansion and drug response profiling of high-grade serous ovarian cancer organoids The broad research use of organoids from high-grade serous ovarian carcinoma (HGSC) has been hampered by low culture success rates and limited availability of fresh tumor material. Here we describe a method for generation and long-term expansion of HGSC organoids with efficacy markedly improved over previous reports (55% vs. 23-38%). We established organoids from cryopreserved material, demonstrating the feasibility of using viably biobanked tissue for HGSC organoid derivation. Genomic, histologic and single-cell transcriptomic analyses revealed that organoids recapitulated genetic and phenotypic features of original tumors. Organoid drug responses correlated with clinical treatment outcomes, although in culture conditions-dependent manner and only in organoids maintained in human plasma-like medium (HPLM). Organoids from consenting patients are available to the research community through a public biobank and organoid genomic data explorable through an interactive online tool. Taken together, this resource facilitates the application of HGSC organoids in basic and translational ovarian cancer research.
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The categories of this paper are: molecular
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The categories of this paper are: evolutionary
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The categories of this paper are: pharmacology
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The categories of this paper are: synthetic
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The categories of this paper are: and
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The categories of this paper are: systems
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The categories of this paper are: cell
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The categories of this paper are: education
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The categories of this paper are: biochemistry
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The categories of this paper are: cognition
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The categories of this paper are: developmental
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The categories of this paper are: neuroscience
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The categories of this paper are: bioinformatics
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The categories of this paper are: genetics
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The categories of this paper are: biophysics
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The categories of this paper are: animal