Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Real-time visualization of mRNA synthesis during memory formation in live animals Memories are thought to be encoded in populations of neurons called memory trace or engram cells. However, little is known about the dynamics of these cells because of the difficulty in real-time monitoring of them over long periods of time in vivo. To overcome this limitation, we present a genetically-encoded RNA indicator (GERI) mouse for intravital chronic imaging of endogenous Arc mRNA--a popular marker for memory trace cells. We used our GERI to identify Arc-positive neurons in real time without the delay associated with reporter protein expression in conventional approaches. We found that the Arc-positive neuronal populations rapidly turned over within two days in the hippocampal CA1 region, whereas [~]4% of neurons in the retrosplenial cortex (RSC) consistently expressed Arc following contextual fear conditioning and repeated memory retrievals. Dual imaging of GERI and a calcium indicator in CA1 of mice navigating a virtual reality environment revealed that only the population of neurons expressing Arc during both encoding and retrieval exhibited relatively high calcium activity in a context-specific manner. This in vivo RNA imaging approach opens the possibility of unraveling the dynamics of the neuronal population underlying various learning and memory processes.

One Sentence SummaryLive-animal imaging of Arc mRNA reveals the dynamics and activity of memory trace cells during memory encoding and retrieval.
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The categories of this paper are: pharmacology
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The categories of this paper are: pathology
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The categories of this paper are: genomics
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The categories of this paper are: immunology
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The categories of this paper are: microbiology
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The categories of this paper are: physiology
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The categories of this paper are: cognition
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The categories of this paper are: toxicology
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The categories of this paper are: ecology
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The categories of this paper are: education
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The categories of this paper are: plant
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The categories of this paper are: developmental
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The categories of this paper are: synthetic
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The categories of this paper are: biology
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The categories of this paper are: cell
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The categories of this paper are: genetics