Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Knockout of circRNAs by base editing back-splice sites of circularized exons A large number of circular RNAs (circRNAs) are produced from back-splicing of exon(s) of precursor mRNAs and generally co-expressed with their cognate linear RNAs from the same gene loci. Methods for circRNA-specific knockout are lacking, largely due to complete sequence-overlaps between circular and cognate linear RNAs. Here, we report to use base editors (BEs) for circRNA depletion. By targeting splice sites involved in both back-splicing and canonical splicing, BEs can repress both circular and linear RNAs expression, which confirms the requirement of canonical splice site signals for back-splice. Importantly, by targeting back-splice sites predominantly for circRNA biogenesis, BEs could efficiently repress the production of circular, but not linear cognate RNAs. As hundreds of exons were found to be predominantly back-spliced to produce circRNAs, this study provides an efficient method to deplete circRNAs for function study.
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The categories of this paper are: biochemistry
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The categories of this paper are: ecology
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The categories of this paper are: animal
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The categories of this paper are: neuroscience
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The categories of this paper are: molecular
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The categories of this paper are: genomics
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The categories of this paper are: pharmacology
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The categories of this paper are: microbiology
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The categories of this paper are: systems
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The categories of this paper are: pathology
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The categories of this paper are: synthetic
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The categories of this paper are: toxicology
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The categories of this paper are: behavior
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The categories of this paper are: scientific
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The categories of this paper are: education
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The categories of this paper are: zoology