Instruct: Identify the main and secondary category of Arxiv papers based on the titles and abstracts
Query: Data Exploration, Quality Control and Testing in Single-Cell qPCR-Based
  Gene Expression Experiments   Cell populations are never truly homogeneous; individual cells exist in
biochemical states that define functional differences between them. New
technology based on microfluidic arrays combined with multiplexed quantitative
polymerase chain reactions (qPCR) now enables high-throughput single-cell gene
expression measurement, allowing assessment of cellular heterogeneity. However
very little analytic tools have been developed specifically for the statistical
and analytical challenges of single-cell qPCR data. We present a statistical
framework for the exploration, quality control, and analysis of single-cell
gene expression data from microfluidic arrays. We assess accuracy and
within-sample heterogeneity of single-cell expression and develop quality
control criteria to filter unreliable cell measurements. We propose a
statistical model accounting for the fact that genes at the single-cell level
can be on (and for which a continuous expression measure is recorded) or
dichotomously off (and the recorded expression is zero). Based on this model,
we derive a combined likelihood-ratio test for differential expression that
incorporates both the discrete and continuous components. Using an experiment
that examines treatment-specific changes in expression, we show that this
combined test is more powerful than either the continuous or dichotomous
component in isolation, or a t-test on the zero-inflated data. While developed
for measurements from a specific platform (Fluidigm), these tools are
generalizable to other multi-parametric measures over large numbers of events.

23
The categories of this paper are: solv-int
4
The categories of this paper are: cond-mat
17
The categories of this paper are: q-fin
33
The categories of this paper are: plasm-ph
36
The categories of this paper are: cmp-lg
21
The categories of this paper are: adap-org
35
The categories of this paper are: mtrl-th
3
The categories of this paper are: physics
11
The categories of this paper are: hep-lat
9
The categories of this paper are: nlin
18
The categories of this paper are: econ
0
The categories of this paper are: hep-ph
8
The categories of this paper are: hep-ex
19
The categories of this paper are: eess
15
The categories of this paper are: nucl-ex
22
The categories of this paper are: chao-dyn