Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Actin crosslinker competition and sorting drive emergent GUV size-dependent actin network architecture How complex actin network architectures arise and coexist in membrane-enclosed cell environment remains unknown. By encapsulating actin and crosslinking proteins -actinin and fascin in giant unilamellar vesicles (GUVs), we show that physical confinement and its size can lead to formation of complex actin structures, including rings and asters at GUV peripheries and centers. Strikingly, we find that the materials properties of the aster structures depend on the ratio of the relative concentrations of -actinin and fascin, and we demonstrate that this results from -actinin and fascin sorting into separate domains in the structures. We complement our experiments with molecular simulations that capture the spontaneous formation of competing network architectures; these provide a microscopic view of the dynamics and delineate the molecular features that promote sorting. We propose that the observed boundary-imposed effect on protein sorting is a general mechanism for creating emergent structures in biopolymer networks with multiple crosslinkers.
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The categories of this paper are: biochemistry
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The categories of this paper are: pharmacology
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The categories of this paper are: molecular
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The categories of this paper are: biology
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The categories of this paper are: zoology
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The categories of this paper are: microbiology
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The categories of this paper are: and
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The categories of this paper are: pathology
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The categories of this paper are: systems
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The categories of this paper are: toxicology
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The categories of this paper are: behavior
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The categories of this paper are: genomics
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The categories of this paper are: plant
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The categories of this paper are: bioinformatics
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The categories of this paper are: genetics
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The categories of this paper are: developmental