Instruct: Identify the main category of Medrxiv papers based on the titles and abstracts
Query: Design and validation of HIV peptide pools for detection of HIV-specific CD4+ and CD8+ T cells Reagents to monitor T cell responses to the entire HIV genome, based on well characterized epitopes, are missing. Evaluation of HIV-specific T cell responses is of importance to study natural infection, and therapeutic and vaccine interventions. Experimentally derived CD4+ and CD8+ HIV epitopes from the HIV molecular immunology database were developed into Class I and Class II HIV megapools (MPs). We assessed HIV responses in persons with HIV pre combined antiretroviral therapy (cART) (n=17) and post-cART (n=18) and compared these responses to 15 controls without HIV (matched by sex, age, and ethnicity). Using the Activation Induced Marker (AIM) assay, we quantified HIV-specific total CD4+, memory CD4+, circulating T follicular helper, total CD8+ and memory CD8+ T cells. We also compared the Class I and Class II HIV MPs to commercially available HIV gag peptide pools. Overall, HIV Class II MP detected HIV-specific CD4+ T cells in 21/35 (60%) HIV positive samples and 0/15 HIV negative samples. HIV Class I MP detected an HIV-specific CD8+ T cells in 17/35 (48.6%) HIV positive samples and 0/15 HIV negative samples. Our innovative HIV MPs are reflective of the entire HIV genome, and its performance is comparable to other commercially available peptide pools. Here, we detected HIV-specific CD4+ and CD8+ T cell responses in people on and off cART, but not in people without HIV.
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The categories of this paper are: nursing
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The categories of this paper are: allergy
42
The categories of this paper are: occupational
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The categories of this paper are: therapeutics
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The categories of this paper are: therapy
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The categories of this paper are: improvement
5
The categories of this paper are: health
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The categories of this paper are: addiction
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The categories of this paper are: epidemiology
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The categories of this paper are: genetic
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The categories of this paper are: pain
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The categories of this paper are: critical
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The categories of this paper are: psychiatry
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The categories of this paper are: surgery
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The categories of this paper are: obstetrics
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The categories of this paper are: dentistry