Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Plasma membrane damage limits replicative lifespan in yeast and human fibroblasts Plasma membrane damage (PMD) occurs in all cell types due to environmental perturbation and cell-autonomous activities. However, cellular outcomes of PMD remain largely unknown except for recovery or death. Here, using yeast and human fibroblasts, we show that cellular senescence, irreversible cell cycle arrest contributing to organismal aging, is the third outcome of PMD. To identify the genes essential for PMD response, we performed a systematic yeast genome-wide screen. The screen identified 48 genes. The top hits in the screen are the endosomal sorting complexes required for transport (ESCRT) genes. Strikingly, the replicative lifespan regulator genes are enriched in our 48 hits, and indeed, PMD limits the replicative lifespan in budding yeast; the ESCRT activator AAA-ATPase VPS4-overexpression extends it. In normal human fibroblasts, PMD induces premature senescence via p53. Our study demonstrates that PMD limits replicative lifespan in two different eukaryotic cell types and highlights an underappreciated but ubiquitous senescent cell subtype, namely PMD-dependent senescent cells.
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The categories of this paper are: biophysics
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The categories of this paper are: bioinformatics
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The categories of this paper are: animal
0
The categories of this paper are: genetics
9
The categories of this paper are: synthetic
32
The categories of this paper are: zoology
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The categories of this paper are: pharmacology
3
The categories of this paper are: evolutionary
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The categories of this paper are: communication
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The categories of this paper are: pathology
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The categories of this paper are: microbiology
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The categories of this paper are: physiology
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The categories of this paper are: education
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The categories of this paper are: and
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The categories of this paper are: plant
21
The categories of this paper are: toxicology