Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Cycles of transcription and local translation support molecular long-term memory in the hippocampus Long-term memory requires transcription and translation of activity-regulated genes. Many of these are immediate early genes (IEGs) with short-lived mRNAs and proteins, decaying rapidly after stimulation. It remains unknown how an IEG with rapid mRNA and protein turnover can impact long-lasting changes at the synapses. Using fluorescently tagged endogenous Arc, an IEG important for memory consolidation, we performed high-resolution imaging of transcription and translation in individual neurons to identify the long-term gene dynamics after stimulation. Unexpectedly, once induced, Arc underwent transcriptional reactivation often at the same allele. Cycles of transcription were coordinated with localized translation. This cyclical regulation of an IEG, dependent on protein synthesis, reactivates subsequent transcription for maintaining mRNA supply to dendrites. The ensuing Arc mRNAs were preferentially localized at sites marked by previous Arc protein, thereby consolidating local "hubs" of dendritic Arc. These findings revealed the spatio-temporal dynamics of transcription-translation coupling of an IEG and provide a mechanism by which short-lived synaptic proteins can be sustained over the long-time scales of memory consolidation.
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The categories of this paper are: toxicology
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The categories of this paper are: pharmacology
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The categories of this paper are: physiology
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The categories of this paper are: cell
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The categories of this paper are: bioengineering
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The categories of this paper are: scientific
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The categories of this paper are: biophysics
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The categories of this paper are: communication
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The categories of this paper are: biochemistry
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The categories of this paper are: molecular
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The categories of this paper are: ecology
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The categories of this paper are: cognition
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The categories of this paper are: systems
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The categories of this paper are: epidemiology
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The categories of this paper are: education
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The categories of this paper are: synthetic