Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Self-organization, quality control, and preclinical studies of human iPSC-derived retinal sheets for tissue-transplantation therapy Three-dimensional retinal organoids (3D-retinas) are a promising graft source for transplantation therapy. We previously developed self-organizing culture for 3D-retina generation from human pluripotent stem cells (hPSCs). Here we present a quality control method and preclinical studies for tissue-sheet transplantation. Self-organizing hPSCs differentiated into both retinal and off-target tissues. Gene expression analyses identified the major off-target tissues as eye-related, cortex-like, and spinal cord-like tissues. For quality control, we developed a qPCR-based test in which each hPSC- derived neuroepithelium was dissected into two tissue-sheets: inner-central sheet for transplantation and outer-peripheral sheet for qPCR to ensure retinal tissue selection. During qPCR, tissue-sheets were stored for 3-4 days using a newly developed preservation method. In a rat tumorigenicity study, no transplant-related adverse events were observed. In retinal degeneration model rats, retinal transplants differentiated into mature photoreceptors and exhibited light responses in electrophysiology assays. These results demonstrate our rationale toward self-organizing retinal sheet transplantation therapy.
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The categories of this paper are: plant
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The categories of this paper are: developmental
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The categories of this paper are: animal
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The categories of this paper are: molecular
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The categories of this paper are: synthetic
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The categories of this paper are: pharmacology
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The categories of this paper are: immunology
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The categories of this paper are: biochemistry
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The categories of this paper are: communication
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The categories of this paper are: bioinformatics
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The categories of this paper are: systems
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The categories of this paper are: cell
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The categories of this paper are: behavior
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The categories of this paper are: biophysics
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The categories of this paper are: and
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The categories of this paper are: paleontology