Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Spatial Epigenome Sequencing at Tissue Scale and Cellular Level Spatial biology is emerging as a new frontier of biomedical research in development and disease, but currently limited to transcriptome and a panel of proteins. Here we present spatial epigenome profiling for three histone modifications (H3K27me3, H3K4me3, H3K27ac) via next-generation sequencing by combining in-tissue CUT&Tag chemistry and microfluidic deterministic barcoding. Spatial chromatin states in mouse embryos or olfactory bulbs revealed tissue type-specific epigenetic regulations, in concordance with ENCODE reference data, but providing spatially resolved genome-wide profiles at tissue scale. Using fluorescence imaging to identify the tissue pixels (20m) each containing one nucleus allowed us to extract single-cell epigenomes in situ. Spatial chromatin state profiling in tissue may enable unprecedented opportunities to study epigenetic regulation, cell function and fate decision in normal physiology and pathogenesis.
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The categories of this paper are: cognition
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The categories of this paper are: biochemistry
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The categories of this paper are: synthetic
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The categories of this paper are: evolutionary
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The categories of this paper are: epidemiology
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The categories of this paper are: microbiology
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The categories of this paper are: behavior
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The categories of this paper are: biophysics
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The categories of this paper are: cancer
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The categories of this paper are: scientific
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The categories of this paper are: education
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The categories of this paper are: developmental
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The categories of this paper are: biology
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The categories of this paper are: cell
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The categories of this paper are: immunology
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The categories of this paper are: systems