Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Optical nanoscopy reveals SARS-CoV-2-induced remodeling of human airway cells A better understanding of host cell remodeling by the coronavirus SARS-CoV-2 is urgently needed to understand viral pathogenesis and guide drug development. Expression profiling and electron microscopy have frequently been used to study virus-host interactions, but these techniques do not readily enable spatial, sub-cellular and molecular analysis of specific cellular compartments. Here, we use diffraction-unlimited fluorescence microscopy to analyze how SARS-CoV-2 infection exploits and repurposes the subcellular architecture of primary human airway cells. Using STED nanoscopy, we detect viral entry factors along the motile cilia of ciliated cells and visualize key aspects of the viral life cycle. Using Tenfold Robust Expansion (TREx) microscopy, we analyze the extensively remodeled three-dimensional ultrastructure of SARS-CoV-2-infected ciliated cells and uncover Golgi fragmentation, emergence of large and atypical multivesicular bodies enclosing viral proteins, ciliary clustering, and remodeling of the apical surface. These results demonstrate a broadly applicable strategy to study how viruses reorganize host cells with spatial and molecular specificity and provide new insights into SARS-CoV-2 infection in primary human cell models.
22
The categories of this paper are: physiology
13
The categories of this paper are: animal
18
The categories of this paper are: systems
25
The categories of this paper are: bioengineering
8
The categories of this paper are: genomics
9
The categories of this paper are: synthetic
15
The categories of this paper are: developmental
27
The categories of this paper are: pathology
31
The categories of this paper are: paleontology
12
The categories of this paper are: behavior
29
The categories of this paper are: education
24
The categories of this paper are: cancer
4
The categories of this paper are: neuroscience
30
The categories of this paper are: scientific
6
The categories of this paper are: plant
11
The categories of this paper are: cognition