Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Mosquito odorant receptor sensitive to natural spatial repellents and inhibitory compounds Previously, we have identified an odorant receptor (OR) from the southern house mosquito Culex quinquefasciatus, CquiOR32, which responded to both odorants (agonists) and inhibitory compounds (antagonists). CquiOR32/CquiOrco-expressing oocytes responded to methyl salicylate and other odorants with inward (regular) currents but gave currents in the reverse direction when challenged with eucalyptol and other inhibitors. To determine whether hitherto unknown ORs show this intrareceptor inhibition, we have now examined two other receptors in the same cluster, CquiOR27 and CquiOR28. We cloned and tested four variants of CquiOR28, but none of the 250 compounds in our panel of odorants, including an Orco ligand candidate (OCL12), elicited inward or outward currents. By contrast, CquiOR27/CquiOrco-expressing oocytes gave robust, dose-dependent inward currents when challenged with {gamma}-octalactone and other odorants. On the other hand, octylamine and other phenolic compounds elicited dose-dependent currents in the reverse direction. When stimulatory and inhibitory compounds were presented in binary mixtures, {gamma}-octalactone-elicited inward currents were attenuated in a dose-dependent manner according to the concentration of octylamine. As part of our chemical ecology approach, we tested the repellency activity of the best ligands in the surface landing and feeding assay and a newly reported hand-in cage assay. Protection elicited by {gamma}-octalactone did not differ significantly from that of DEET at the same dose. In the hand-in cage assay, a cream formulation of {gamma}-octalactone showed 97.0 {+/-} 1.3% protection, with 47.6 {+/-} 8.3% and 1.4 {+/-} 0.7% landings per trial in the hands covered with a control and {gamma}-octalactone cream, respectively (N=8, p=0.0078, Wilcoxon matched-pairs signed-rank test).
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The categories of this paper are: biology
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The categories of this paper are: paleontology
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The categories of this paper are: epidemiology
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The categories of this paper are: biophysics
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The categories of this paper are: genomics
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The categories of this paper are: scientific
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The categories of this paper are: ecology
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The categories of this paper are: synthetic
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The categories of this paper are: bioinformatics
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The categories of this paper are: behavior
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The categories of this paper are: bioengineering
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The categories of this paper are: genetics
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The categories of this paper are: molecular
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The categories of this paper are: animal
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The categories of this paper are: developmental
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The categories of this paper are: evolutionary