Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Monitoring extracellular ion and metabolite dynamics with recombinant nanobody-fused biosensors The tumor microenvironment (TME) consists of different cell types that secrete proteins and also control the extracellular concentration of ions and metabolites. Changes in these intra-tumoral analytes and conditions, including K+, glucose, and pH, have been described to alter the metabolic activity of cancer cells, promote tumor cell growth, and impair anti-tumor immunity. However, the mechanisms regulating ion and metabolite levels and their effects on certain characteristics of the TME are still poorly understood. Therefore, accurate determination and visualization of analyte or state changes in real time within the TME is desired.

In this study, we genetically combined FRET-based fluorescent biosensors with nanobodies (Nbs) and used them for targeted visualization and monitoring of extracellular changes in K+, pH, and glucose on cell surfaces. We demonstrated that these recombinant biosensors quantitatively visualize extracellular K+ alterations on multiple cancer and non-cancer cell lines and primary neurons. By implementing a HER2 specific Nb, we generated K+ and pH sensors, which retain their functionality and specifically stained HER2 positive breast cancer cells. Based on the successful technical development of several Nb-biosensor combinations, we anticipate that this approach can be easily extended to design other targeted biosensors. Such versatile probes will open new possibilities for the reliable study of extracellular analytes in advanced 3D cell models or even in vivo systems.
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The categories of this paper are: cancer
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The categories of this paper are: bioengineering
13
The categories of this paper are: animal
1
The categories of this paper are: bioinformatics
31
The categories of this paper are: paleontology
10
The categories of this paper are: and
14
The categories of this paper are: biophysics
32
The categories of this paper are: zoology
6
The categories of this paper are: plant
22
The categories of this paper are: physiology
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The categories of this paper are: pathology
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The categories of this paper are: toxicology
15
The categories of this paper are: developmental
0
The categories of this paper are: genetics
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The categories of this paper are: systems
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The categories of this paper are: epidemiology