Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: First come, first served: Superinfection exclusion in Deformed wing virus is dependent upon sequence identity and not the order of virus acquisition. Deformed wing virus (DWV) is the most important globally distributed pathogen of honey bees and, when vectored by the ectoparasite Varroa destructor, is associated with high levels of colony losses. Divergent DWV types may differ in their pathogenicity and are reported to exhibit superinfection exclusion upon sequential infections, an inevitability in a Varroa-infested colony. We used a reverse genetic approach to investigate competition and interactions between genetically distinct or related virus strains, analysing viral load over time, tissue distribution with reporter gene-expressing viruses and recombination between virus variants. Transient competition occurred irrespective of the order of virus acquisition, indicating no directionality or dominance. Over longer periods, the ability to compete with a pre-existing infection correlated with the genetic divergence of the inoculae. Genetic recombination was observed throughout the DWV genome with recombinants accounting for ~2% of the population as determined by deep sequencing. We propose that superinfection exclusion, if it occurs at all, is a consequence of a cross-reactive RNAi response to the viruses involved, explaining the lack of dominance of one virus type over another. A better understanding of the consequences of dual- and superinfection will inform development of cross-protective honey bee vaccines and landscape-scale DWV transmission and evolution.
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The categories of this paper are: biochemistry
32
The categories of this paper are: zoology
10
The categories of this paper are: and
5
The categories of this paper are: cell
8
The categories of this paper are: genomics
1
The categories of this paper are: bioinformatics
2
The categories of this paper are: biology
25
The categories of this paper are: bioengineering
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The categories of this paper are: scientific
0
The categories of this paper are: genetics
12
The categories of this paper are: behavior
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The categories of this paper are: paleontology
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The categories of this paper are: communication
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The categories of this paper are: biophysics
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The categories of this paper are: plant
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The categories of this paper are: cognition