Instruct: Identify the main category of Medrxiv papers based on the titles and abstracts
Query: Toward Community Surveillance: Detecting Intact SARS-CoV-2 Using Exogeneous Oligonucleotide Labels The persistence of the COVID-19 pandemic demands a dramatic increase in testing efficiency. Testing pooled samples for SARS-CoV-2 could meet this need; however, the sensitivity of RT-qPCR, the gold standard, significantly decreases with an increasing number of samples pooled. Here, we introduce DIVER, a method that quantifies intact virus and is robust to sample dilution. DIVER first tags viral particles with exogeneous oligonucleotides, then captures the tagged particles on ACE2-functionalized beads, and finally quantifies the oligonucleotide tags using qPCR. Using spike-presenting liposomes and Spike-pseudotyped lentivirus as SARS-CoV-2 models, we show that DIVER can detect 1x105 liposomes and 100 pfu lentivirus and can successfully identify positive samples in pooling experiments. Overall, DIVER is well-positioned for efficient sample pooling and expanded community surveillance.
15
The categories of this paper are: allergy
57
The categories of this paper are: primary
3
The categories of this paper are: sexual
21
The categories of this paper are: psychology
18
The categories of this paper are: global
65
The categories of this paper are: oral
17
The categories of this paper are: informatics
16
The categories of this paper are: immunology
72
The categories of this paper are: urology
69
The categories of this paper are: palliative
42
The categories of this paper are: occupational
63
The categories of this paper are: pain
27
The categories of this paper are: quality
62
The categories of this paper are: orthopedics
73
The categories of this paper are: toxicology
70
The categories of this paper are: otolaryngology