Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: miR-329 and miR-495-mediated Prr7 downregulation isrequired for homeostatic synaptic depression in rathippocampal neurons Homeostatic synaptic depression (HSD) in excitatory neurons is a cell autonomous mechanism which protects excitatory neurons from over-excitation as a consequence of chronic increases in network activity. In this process, excitatory synapses are weakened and eventually eliminated, as evidenced by a reduction in synaptic AMPA receptor expression and dendritic spine loss. Originally considered a global, cell-wide mechanism, local forms of regulation, such as the local control of mRNA translation in dendrites, are being increasingly recognized in HSD. Yet, identification of excitatory proteins whose local regulation is required for HSD is still limited. Here, we show that Proline-rich protein 7/Transmembrane Adapter Protein 3 (Prr7) downregulation in dendrites of rat hippocampal neurons is necessary for HSD induced by chronic increase in network activity resulting from a blockade of inhibitory synaptic transmission by picrotoxin (PTX). We further identify two activity-regulated miRNAs, miR-329-3p and miR-495-3p, which inhibit Prr7 mRNA translation and are required for HSD. Moreover, we found that Prr7 knockdown reduces expression of the synaptic scaffolding protein SPAR, which is rescued by pharmacological inhibition of CDK5, indicating a role of Prr7 protein in the maintenance of excitatory synapses via protection of SPAR from degradation. Together, our findings highlight a novel HSD mechanism in which chronic activity leads to miR-329 and miR-495-mediated local Prr7 reduction upstream of the CDK5-SPAR pathway.
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The categories of this paper are: ecology
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The categories of this paper are: bioinformatics
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The categories of this paper are: pharmacology
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The categories of this paper are: biochemistry
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The categories of this paper are: epidemiology
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The categories of this paper are: zoology
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The categories of this paper are: behavior
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The categories of this paper are: immunology
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The categories of this paper are: plant
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The categories of this paper are: communication
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The categories of this paper are: evolutionary
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The categories of this paper are: molecular
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The categories of this paper are: bioengineering
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The categories of this paper are: cognition
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The categories of this paper are: developmental
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The categories of this paper are: and