Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Ligand-induced unfolding mechanism of an RNA G-quadruplex The cationic porphyrin, TMPyP4, is a well-established DNA G-quadruplex (G4) binding ligand that can stabilize different topologies via multiple binding modes. However, TMPyP4 has completely opposite destabilizing and unwinding effect on RNA G4 structures. The structural mechanisms that mediate RNA G4 unfolding remains unknown. Here, we report on the TMPyP4-induced RNA G4 unfolding mechanism studied by well-tempered metadynamics (WT-MetaD) with supporting biophysical experiments. The simulations predict a two-state mechanism of TMPyP4 interaction via a groove-bound and a top-face bound conformation. The dynamics of TMPyP4 stacking on the top tetrad disrupts Hoogsteen H-bonds between guanine bases resulting in the consecutive TMPyP4 intercalation from top-to-bottom G-tetrads. The results reveal a striking correlation between computational and experimental approaches and validate WT-MetaD simulations as a powerful tool for studying RNA G4-ligand interactions.
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The categories of this paper are: ecology
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The categories of this paper are: education
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The categories of this paper are: epidemiology
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The categories of this paper are: cell
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The categories of this paper are: evolutionary
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The categories of this paper are: systems
32
The categories of this paper are: zoology
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The categories of this paper are: cognition
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The categories of this paper are: developmental
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The categories of this paper are: genomics
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The categories of this paper are: toxicology
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The categories of this paper are: synthetic
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The categories of this paper are: physiology
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The categories of this paper are: bioinformatics
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The categories of this paper are: paleontology
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The categories of this paper are: behavior