Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Nucleosome conformation dictates the histone code Histone post-translational modifications (PTMs) play a critical role in chromatin regulation. It has been proposed that these PTMs form localized  codes that are read by specialized regions (reader domains) in chromatin associated proteins (CAPs) to regulate downstream function. Substantial effort has been made to define [CAP-histone PTM] specificity, and thus decipher the histone code / guide epigenetic therapies. However, this has largely been done using a reductive approach of isolated reader domains and histone peptides, with the assumption that PTM readout is unaffected by any higher order factors. Here we show that CAP-histone PTM interaction is in fact dependent on nucleosome context. Our results indicate this is due to histone tail accessibility and the associated impact on binding potential of reader domains. We further demonstrate that the in vitro specificity of a tandem reader for PTM-defined nucleosomes is recapitulated in a cellular context. This necessitates we refine the  histone code concept and interrogate it at the nucleosome level.
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The categories of this paper are: cancer
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The categories of this paper are: immunology
5
The categories of this paper are: cell
13
The categories of this paper are: animal
7
The categories of this paper are: ecology
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The categories of this paper are: synthetic
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The categories of this paper are: and
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The categories of this paper are: molecular
12
The categories of this paper are: behavior
28
The categories of this paper are: communication
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The categories of this paper are: pharmacology
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The categories of this paper are: zoology
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The categories of this paper are: scientific
31
The categories of this paper are: paleontology
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The categories of this paper are: neuroscience
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The categories of this paper are: systems