Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Direct lysis RT-qPCR of SARS-CoV-2 in cell culture supernatant allows for fast and accurate quantification of virus, opening a vast array of applications An enormous global effort is being made to study SARS-CoV-2 and develop safe and effective treatments. Studying the entire virus replication cycle of SARS-CoV-2 is essential to identify host factors and treatments to combat the infection. However, quantification of released virus often requires lengthy procedures, such as endpoint dilution assays or reinfection with engineered reporter viruses. Quantification of viral RNA in cell supernatant is faster and can be performed on clinical isolates. However, viral RNA purification is expensive in time and resources and often unsuitable for high-throughput screening. Here, we show a direct lysis RT-qPCR method allowing sensitive, accurate, fast, and cheap quantification of SARS-CoV-2 in culture supernatant. During lysis, the virus is completely inactivated, allowing further processing in low containment areas. This protocol facilitates a wide array of high- and low-throughput applications from basic quantification to studying the biology of SARS-CoV-2 and to identify novel antiviral treatments in vitro.
0
The categories of this paper are: genetics
8
The categories of this paper are: genomics
5
The categories of this paper are: cell
26
The categories of this paper are: biochemistry
15
The categories of this paper are: developmental
14
The categories of this paper are: biophysics
21
The categories of this paper are: toxicology
4
The categories of this paper are: neuroscience
19
The categories of this paper are: epidemiology
10
The categories of this paper are: and
11
The categories of this paper are: cognition
27
The categories of this paper are: pathology
18
The categories of this paper are: systems
17
The categories of this paper are: molecular
28
The categories of this paper are: communication
12
The categories of this paper are: behavior