Instruct: Identify the main and secondary category of Arxiv papers based on the titles and abstracts
Query: Multiplex stimulated Raman scattering imaging cytometry reveals cancer
  metabolic signatures in a spatially, temporally, and spectrally resolved
  manner   In situ measurement of cellular metabolites is still a challenge in biology.
Conventional methods, such as mass spectrometry or fluorescence microscopy,
would either destruct the sample or introduce strong perturbations to the
functions of target molecules. Here, we present multiplex stimulated Raman
scattering (SRS) imaging cytometry as a label-free single-cell analysis
platform with chemical specifity, and high-throughput capabilities. Cellular
compartments such as lipid droplets, endoplasmic reticulum, and nuclei are
seperated from the cytoplasm. Based on these chemical segmentations, 260
features from both morphology and molecular composition were generated and
analyzed for each cell. Using SRS imaging cytometry, we studied the metabolic
responses of human pancreatic cancer cells under stress by starvation and
chemotherapy drug treatments. We unveiled lipid-facilitated protrusion as a
metabolic marker for stress-resistant cancer cells through statistical analysis
of thousands of cells. Our findings also demonstrate the potential of targeting
lipid metabolism for selective treatment of starvation-resistant and
chemotherapy-resistant cancers. These results highlight our SRS imaging
cytometry as a powerful label-free tool for biological discoveries with a
high-throughput, high-content capacity.

26
The categories of this paper are: bayes-an
7
The categories of this paper are: hep-th
6
The categories of this paper are: astro-ph
9
The categories of this paper are: nlin
33
The categories of this paper are: plasm-ph
32
The categories of this paper are: atom-ph
1
The categories of this paper are: cs
21
The categories of this paper are: adap-org
31
The categories of this paper are: ao-sci
8
The categories of this paper are: hep-ex
11
The categories of this paper are: hep-lat
35
The categories of this paper are: mtrl-th
20
The categories of this paper are: acc-phys
15
The categories of this paper are: nucl-ex
23
The categories of this paper are: solv-int
16
The categories of this paper are: stat