Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Post-entry, spike-dependent replication advantage of B.1.1.7 and B.1.617.2 over B.1 SARS-CoV-2 in an ACE2-deficient human lung cell line Epidemiological data demonstrate that SARS-CoV-2 variants of concern (VOC) B.1.1.7 and B.1.617.2 are more transmissible and infections are associated with a higher mortality than non-VOC virus infections. Phenotypic properties underlying their enhanced spread in the human population remain unknown. B.1.1.7 virus isolates displayed inferior or equivalent spread in most cell lines and primary cells compared to an ancestral B.1 SARS-CoV-2, and were outcompeted by the latter. Lower infectivity and delayed entry kinetics of B.1.1.7 viruses were accompanied by inefficient proteolytic processing of spike. B.1.1.7 viruses failed to escape from neutralizing antibodies, but slightly dampened induction of innate immunity. The bronchial cell line NCI-H1299 supported 24- and 595-fold increased growth of B.1.1.7 and B.1.617.2 viruses, respectively, in the absence of detectable ACE2 expression and in a spike-determined fashion. Superior spread in NCI-H1299 cells suggests that VOCs employ a distinct set of cellular cofactors that may be unavailable in standard cell lines.
26
The categories of this paper are: biochemistry
2
The categories of this paper are: biology
17
The categories of this paper are: molecular
11
The categories of this paper are: cognition
29
The categories of this paper are: education
27
The categories of this paper are: pathology
20
The categories of this paper are: pharmacology
0
The categories of this paper are: genetics
9
The categories of this paper are: synthetic
24
The categories of this paper are: cancer
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The categories of this paper are: epidemiology
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The categories of this paper are: bioengineering
22
The categories of this paper are: physiology
32
The categories of this paper are: zoology
14
The categories of this paper are: biophysics
6
The categories of this paper are: plant