Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Citrullination of a phage displayed human peptidome library reveals the fine specificities of rheumatoid arthritis-associated autoantibodies Post-translational modifications (PTMs) on proteins can be targeted by antibodies associated with autoimmunity. Despite a growing appreciation for their intrinsic role in disease, there is a lack of highly multiplexed serological assays to characterize the fine specificities of PTM-directed autoantibodies. In this study, we used the programmable phage display technology, Phage ImmunoPrecipitation Sequencing (PhIP-Seq), to profile rheumatoid arthritis (RA) associated anti-citrullinated protein antibody (ACPA) reactivities. Using both an unmodified and peptidylarginine deiminases (PAD)-modified phage display library consisting of ~250,000 overlapping 90 amino acid peptide tiles spanning the human proteome, PTM PhIP-Seq robustly identifies antibodies to citrulline-dependent epitopes. PTM PhIP-Seq was used to quantify key differences among RA patients, including PAD isoform specific ACPA profiles, and thus represents a powerful tool for proteome-scale antibody-binding analyses.
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The categories of this paper are: developmental
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The categories of this paper are: scientific
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The categories of this paper are: animal
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The categories of this paper are: zoology
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The categories of this paper are: ecology
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The categories of this paper are: behavior
25
The categories of this paper are: bioengineering
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The categories of this paper are: genetics
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The categories of this paper are: genomics
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The categories of this paper are: and
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The categories of this paper are: pathology
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The categories of this paper are: molecular
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The categories of this paper are: microbiology
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The categories of this paper are: epidemiology
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The categories of this paper are: toxicology
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The categories of this paper are: biophysics