Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Compartimentalized control of Cdk1 drives mitotic spindle assembly During cell division, dramatic microtubular rearrangements driven by cyclin B-cdk1 (Cdk1) kinase activity mark mitosis onset leading to interphase cytoskeleton dissolution and mitotic spindle assembly. Once activated by Cdc25, that reverses inhibitory phosphorylation operated by Wee1/Myt1, Cdk1 clears the cytoplasm from microtubules by inhibiting microtubule associated proteins (MAPs) with microtubule growth-promoting properties. Nevertheless, some of these MAPs are required for spindle assembly, creating quite a conundrum. We show here that a Cdk1 fraction bound to spindle structures escaped Cdc25 action and remained inhibited by phosphorylation (i-Cdk1) in mitotic human cells. Loss or restoration of i-Cdk1 inhibited or promoted spindle assembly, respectively. Furthermore, polymerizing spindle microtubules fostered i-Cdk1 by aggregating with Wee1 and excluding Cdc25. Our data reveal that spindle assembly relies on compartmentalized control of Cdk1 activity.
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The categories of this paper are: pharmacology
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The categories of this paper are: ecology
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The categories of this paper are: systems
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The categories of this paper are: biochemistry
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The categories of this paper are: biophysics
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The categories of this paper are: immunology
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The categories of this paper are: synthetic
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The categories of this paper are: toxicology
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The categories of this paper are: physiology
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The categories of this paper are: pathology
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The categories of this paper are: genomics
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The categories of this paper are: scientific
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The categories of this paper are: bioinformatics
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The categories of this paper are: neuroscience
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The categories of this paper are: plant
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The categories of this paper are: evolutionary