Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Dissecting the subtropical adaptation traits and cuticle synthesis pathways via the genome of the subtropical blueberry Vaccinium darrowii Vaccinium darrowii is a subtropical wild blueberry species, which was used to breed economically important southern highbush cultivars. The adaptation traits of V. darrowii to subtropical climate would provide valuable information for breeding blueberry and perhaps other plants, especially against the background of global warming. Here, we assembled the V. darrowii genome into 12 pseudochoromosomes using Oxford Nanopore long reads complemented with Hi-C scaffolding technologies, and predicted 41 815 genes using RNAseq evidence. Syntenic analysis across three Vaccinium species revealed a highly conserved genome structure, with the highest collinearity between V. darrowii and V. corymbosum. This conserved genome structure may explain the high fertilization during crossbreeding between V. darrowii and other blueberry cultivars. Gene expansion and tandem duplication analysis indicated possible roles of defense and flowering associated genes in adaptation of V. darrowii to the subtropics. The possible SOC1 genes in V. darrowii were identified with phylogeny and expression analysis. Blueberries are covered in a thick cuticle layer and contain anthocyanins, which confer their powdery blue color. Using RNA-sequencing, the cuticle biosynthesis pathways of Vaccinium species were delineated here in V. darrowii. This result could serve as a reference for breeding berries with customer-desired colors. The V. darrowii reference genome, together with the unique traits of this species, including diploid genome, short vegetative phase, and high compatibility in hybridization with other blueberries, make V. darrowii a potential research model for blueberry species.
12
The categories of this paper are: behavior
28
The categories of this paper are: communication
14
The categories of this paper are: biophysics
11
The categories of this paper are: cognition
13
The categories of this paper are: animal
24
The categories of this paper are: cancer
5
The categories of this paper are: cell
9
The categories of this paper are: synthetic
3
The categories of this paper are: evolutionary
10
The categories of this paper are: and
0
The categories of this paper are: genetics
21
The categories of this paper are: toxicology
25
The categories of this paper are: bioengineering
32
The categories of this paper are: zoology
26
The categories of this paper are: biochemistry
19
The categories of this paper are: epidemiology