Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Complexities in the role of acetylation dynamics in modifying inducible gene activation parameters High levels of histone acetylation are associated with the regulatory elements of active genes, suggesting a link between acetylation and gene activation. However, several studies have shown that histone acetylation dynamics rather than hyperacetylation per se are important determinants in gene activation, particularly at inducible genes. We revisited this model, in the context of EGF-inducible gene expression and found that rather than a simple unifying model, there are two broad classes of genes; one in which high lysine acetylation activity is required for efficient gene activation, and a second group where the opposite occurs and high acetylation activity is inhibitory. We examined the latter class in more detail using EGR2 as a model gene and found that lysine acetylation levels are critical for several activation parameters, including the timing of expression onset, and overall amplitudes of the transcriptional response. In contrast, DUSP1 responds in the canonical manner and its transcriptional activity is promoted by acetylation. Single cell approaches demonstrate heterogenous DUSP1 activation kinetics and that acetylation levels influence allele activation frequencies. Our data therefore point to a complex interplay between acetylation dynamics and target gene induction, which cannot simply be explained by a unified response to acetylation activity. Instead, acetylation level thresholds are an important determinant of transcriptional induction dynamics that are sensed in a gene-specific manner.
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The categories of this paper are: epidemiology
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The categories of this paper are: systems
29
The categories of this paper are: education
7
The categories of this paper are: ecology
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The categories of this paper are: scientific
28
The categories of this paper are: communication
32
The categories of this paper are: zoology
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The categories of this paper are: developmental
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The categories of this paper are: microbiology
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The categories of this paper are: paleontology
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The categories of this paper are: pharmacology
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The categories of this paper are: neuroscience
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The categories of this paper are: bioinformatics
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The categories of this paper are: pathology
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The categories of this paper are: cognition
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The categories of this paper are: synthetic