Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Mitochondrial structure and function adaptation in residual triple negative breast cancer cells surviving chemotherapy treatment Neoadjuvant chemotherapy (NACT) used for triple negative breast cancer (TNBC) eradicates tumors in approximately 45% of patients. Unfortunately, TNBC patients with substantial residual cancer burden have poor metastasis free and overall survival rates. We previously demonstrated mitochondrial oxidative phosphorylation (OXPHOS) was elevated and was a unique therapeutic dependency of residual TNBC cells surviving NACT. We sought to investigate the mechanism underlying this enhanced reliance on mitochondrial metabolism. Mitochondria are morphologically plastic organelles that cycle between fission and fusion to maintain mitochondrial integrity and metabolic homeostasis. The functional impact of mitochondrial structure on metabolic output is highly context dependent and not understood in TNBC. Several chemotherapy agents are conventionally used for neoadjuvant treatment of TNBC patients. Upon comparing mitochondrial effects of commonly used chemotherapies, we found that DNA-damaging agents increased mitochondrial elongation, mitochondrial content, flux of glucose through the TCA cycle, and OXPHOS, whereas taxanes instead decreased mitochondrial elongation and OXPHOS. Additionally, short protein isoform levels of the mitochondrial inner membrane fusion protein optic atrophy 1 (OPA1) were associated with those observations. Further, we observed heightened OXPHOS, OPA1 protein levels, and mitochondrial elongation in a patient-derived xenograft (PDX) model of residual TNBC. Pharmacologic or genetic disruption of mitochondrial fusion and fission resulted in decreased or increased OXPHOS, respectively, revealing that longer mitochondria favor oxphos in TNBC cells. Using TNBC cell lines and an in vivo PDX model of residual TNBC, we found that sequential treatment with DNA-damaging chemotherapy, thus inducing mitochondrial fusion and OXPHOS, followed by MYLS22, a specific inhibitor of OPA1, was able to suppress mitochondrial fusion and OXPHOS and significantly inhibited residual tumor regrowth. Taken together, our findings suggest that TNBC mitochondria can optimize OXPHOS through modulation of mitochondrial structure. This may provide an opportunity to overcome mitochondrial adaptations of chemoresistant TNBC.
15
The categories of this paper are: developmental
17
The categories of this paper are: molecular
16
The categories of this paper are: microbiology
30
The categories of this paper are: scientific
29
The categories of this paper are: education
4
The categories of this paper are: neuroscience
1
The categories of this paper are: bioinformatics
0
The categories of this paper are: genetics
14
The categories of this paper are: biophysics
9
The categories of this paper are: synthetic
5
The categories of this paper are: cell
26
The categories of this paper are: biochemistry
3
The categories of this paper are: evolutionary
23
The categories of this paper are: immunology
28
The categories of this paper are: communication
6
The categories of this paper are: plant