Instruct: Identify the main category of Biorxiv papers based on the titles and abstracts
Query: Structure and Stability of the designer protein WRAP-T and its permutants {beta}-Propeller proteins are common natural disc-like pseudo-symmetric proteins that contain multiple repeats ( blades) each consisting of a 4-stranded anti-parallel {beta}-sheet. So far, 4- to 12-bladed {beta}-propellers have been discovered in nature showing large functional and sequential variation. Using computational design approaches, we created perfectly symmetric {beta}-propellers out of natural pseudo-symmetric templates. These proteins are useful tools to study protein evolution of this very diverse fold. While the 7-bladed architecture is the most common, no symmetric 7-bladed monomer has been created and characterized so far. Here we describe such a engineered protein, based on a highly symmetric natural template, and test the effects of circular permutation on its stability. Geometrical analysis of this protein and other artificial symmetrical proteins reveals no systematic constraint that could be used to help in engineering of this fold, and suggests sequence constraints unique to each {beta}-propeller sub-family.
21
The categories of this paper are: toxicology
24
The categories of this paper are: cancer
19
The categories of this paper are: epidemiology
4
The categories of this paper are: neuroscience
22
The categories of this paper are: physiology
11
The categories of this paper are: cognition
15
The categories of this paper are: developmental
7
The categories of this paper are: ecology
13
The categories of this paper are: animal
5
The categories of this paper are: cell
25
The categories of this paper are: bioengineering
28
The categories of this paper are: communication
6
The categories of this paper are: plant
27
The categories of this paper are: pathology
9
The categories of this paper are: synthetic
23
The categories of this paper are: immunology