how does etbr stain rna?
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How does EtBr stain RNA in a denaturing RNA gel electrophoresis? In the denaturing RNA gel electrophoresis, by the action of used formamide or formaldehyde the secondary structures of RNA (hair pin loop, pseudo double helix etc.)
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The most basic difference between RER and SER is the presence of ribosomes. When ribosomes attach to the surface of an ER, it gives a characteristic rough appearance; hence it is called Rough ER. On the other hand, a smooth ER does not have ribosomes on its surface. It possesses ribosomes attached to its membrane.
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Description of Tests Assay steps include viral RNA extraction, reverse transcription, and detection by real-time PCR. The assays detect a conserved region of the HIV-2 LTR repeat region. HIV-2 standards are included to produce quantitative results that are reported in HIV-2 RNA international units (IU) /ml.
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Basic: A stain that has positively charged ions (cationic) that will react with material that is negatively charged. The surface of bacteria is somewhat negatively charged, therefore basic dyes will permeate the cell wall and will be attracted to negative charges of the bacteria and stain the bacteria.
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Its job is to match an mRNA codon with the amino acid it codes for. You can think of it as a kind of molecular "bridge" between the two. Each tRNA contains a set of three nucleotides called an anticodon. The anticodon of a given tRNA can bind to one or a few specific mRNA codons.
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Eosin can be used to stain cytoplasm, red blood cells, collagen, and muscle fibers for histological examination. It is most often used as a counterstain to hematoxylin in H&E staining.
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Prokaryotes utilize one RNA polymerase for all transcription of types of RNA. In contrast, eukaryotes utilize three slightly different RNA polymerases: RNA polymerase I, RNA polymerase II, and RNA polymerase III (8). Each of the three RNA polymerases in eukaryotes is responsible for transcribing a unique type of RNA.
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In molecular biology, TBST (or TTBS) is a mixture of tris-buffered saline (TBS) and Polysorbate 20 (also known as Tween 20). It is a buffer used for washing nitrocellulose membrane in western blotting and microtiter plate wells in ELISA assays.
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The MTT assay involves the conversion of the water-soluble yellow dye MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] to an insoluble purple formazan by the action of mitochondrial reductase. Formazan is then solubilized and the concentration determined by optical density at 570 nm.
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A simple stain will generally make all of the organisms in a sample appear to be the same color, even if the sample contains more than one type of organism. In contrast, differential staining distinguishes organisms based on their interactions with multiple stains.
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RNA-dependent RNA polymerase. The RdRp catalyzes the synthesis of RNA using an RNA template. Two features have made this enzymic activity difficult to study. First, it is usually associated with membrane structures in the cell and, on isolation, the enzyme(s) often become(s) unstable.
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EtBr intercalation interferes with the protamine-DNA interaction, challenging the distortion of the DNA helix and separation of DNA base pairs by protamine. Thus, EtBr, by competing directly with protamine, resists the phenomenon of DNA condensation.
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RNA molecules are transported from the nucleus to the cytoplasm in eukaryotes through: sodium/potassium channels. aquaporins.
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A transfer RNA (tRNA) is a special kind of RNA molecule. ... Each tRNA contains a set of three nucleotides called an anticodon. The anticodon of a given tRNA can bind to one or a few specific mRNA codons. The tRNA molecule also carries an amino acid: specifically, the one encoded by the codons that the tRNA binds.
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Internal structural elements of a biological specimen are often stained with a mixture of dyes to selectively stain these elements, increasing their contrast against a background of material that is either transparent or stained a different color.
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Attached. Attached, or bound, ribosomes are found on the exterior of an organelle called the endoplasmic reticulum. Because the ribosomes give the organelle a lumpy appearance, the ribosome-containing portion is called the rough ER.
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Reverse transcription PCR, or RT-PCR, allows the use of RNA as a template. An additional step allows the detection and amplification of RNA. The RNA is reverse transcribed into complementary DNA (cDNA), using reverse transcriptase. ... From here on, the standard PCR procedure is used to amplify the cDNA.