what of these are the two main functions of the dna polymerase?
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The main function of DNA polymerase is to synthesize DNA from deoxyribonucleotides, the building blocks of DNA. ... Primers consist of RNA or DNA bases (or both). In DNA replication, the first two bases are always RNA, and are synthesized by another enzyme called primase.
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It is now well known that DNA polymerases synthesize new DNA strands according to the template DNA, using deoxynucleotide triphosphates during DNA replication. DNA ligase works to join the Okazaki fragment during the lagging strand synthesis in semiconservative DNA replication.
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Introduction• Multiple proteins are required for DNA replication at a replication fork. These include DNA polymerases, single-strand DNA binding proteins, helicases, primase,topoisomerases, and DNA ligase. Some of these are multisubunit protein complexes.
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The physiological function of Pol I is mainly to repair any damage with DNA, but it also serves to connect Okazaki fragments by deleting RNA primers and replacing the strand with DNA.
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DNA polymerase and DNA ligase are ubiquitous enzymes that synthesize complementary DNA strands according to the template DNA and ligate breaks in the backbone structure of DNA, respectively, in living organisms.
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Both synthesize in the 5' to 3' direction, anti-parallel to the template. DNA polymerase requires a primer, but RNA polymerase can start a nucleotide chain from scratch. DNA polymerase used thymine, whereas RNA uses uracil. ... The region of DNA to which RNA polymerase binds to begin transcription.
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DNA polymerase is responsible for the process of DNA replication, during which a double-stranded DNA molecule is copied into two identical DNA molecules. Scientists have taken advantage of the power of DNA polymerase molecules to copy DNA molecules in test tubes via polymerase chain reaction, also known as PCR.
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Taq polymerase catalyzes the generation of new pieces of ssDNA that are complimentary to the portions marked by the primers. The job of Taq polymerase is to move along the strand of DNA and use it as a template for assembling a new strand that is complimentary to the template.
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DNA serves two important cellular functions: It is the genetic material passed from parent to offspring and it serves as the information to direct and regulate the construction of the proteins necessary for the cell to perform all of its functions.
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One of the key molecules in DNA replication is the enzyme DNA polymerase. DNA polymerases are responsible for synthesizing DNA: they add nucleotides one by one to the growing DNA chain, incorporating only those that are complementary to the template.
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What is an important difference in the enzymatic activity between DNA polymerases I and III? Only DNA polymerase I has 5′-to-3′ exonuclease activity. ... DNA polymerases cannot begin without a primer containing a free 3 ′-OH. On the lagging strand, primase builds an RNA primer in short bursts.
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RNA polymerase does not play a role in DNA replication, it plays a role in DNA transcription. RNA polymerase makes mRNA from DNA.
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The two enzymes synthesize the DNA in the 5'-3' direction. DNA polymerase III synthesizes DNA on leading strand in a continuous manner, while DNA polymerase I have 5'-3' exonuclease activity which helps it in DNA repair, in multiple primer removals on lagging strand.
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['Replication. Replication of plasmid DNA is carried by the same enzymes that replicate the E. ... ', 'Selectable Markers (Antibiotic resistance) ... ', 'Multiple cloning sites (or polylinkers) ... ', 'Single-stranded DNA production. ... ', 'Bacteriophage promoters. ... ', 'Miniature Preparation of Bacterial Plasmid.']
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DNA polymerase adds a new strand of DNA by extending the 3′ end of an existing nucleotide chain, adding new nucleotides matched to the template strand one at a time via the creation of phosphodiester bonds.
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Taq Polymerase is Preferred Enzyme for Polymerase Chain Reaction (PCR) A DNA Polymerase is a vital biological enzyme that is present in DNA replication. In the process, DNA copies into two daughter DNA molecules and synthesizes a new DNA strand from the existing strand by adding dNTPs to the growing DNA.
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RNA polymerase (green) synthesizes RNA by following a strand of DNA. RNA polymerase is an enzyme that is responsible for copying a DNA sequence into an RNA sequence, duyring the process of transcription. ... RNA polymerases have been found in all species, but the number and composition of these proteins vary across taxa.