For the Menidia beryllina test method , the effluent sample was an industrial wastewater spiked with CuSO4 , the receiving water sample was a natural seawater spiked with CuSO4 , and the reference toxicant sample was bioassay-grade FORTY FATHOMSa synthetic seawater spiked with CuSO4 .
There were reports of the solution causing side effects .