What is the reason for using different amounts of supernatant with cytolysins depending on its source or the type of cell line it is tested against in figures 2 and 3?
In the material and methods section it is stated that the cells survival assays start with the addition of a fixed volume of supernatant but it is not clear to me the criteria for reflecting different amounts of supernatant in Figures 2 and 3. It should be explained more clearly. What is the reason for using different amounts of supernatant with cytolysins depending on its source or the type of cell line it is tested against in figures 2 and 3? This should be explained. Furthermore, it should be justified why this difference does not influence the comparison of the results with the different cell lines (THP1, Jurkat, Raji).