A number of improvements of the experimental procedure for the thermally induced shape fluctuation method were used in this study: the stroboscopic illumination was improved on several steps to achieve a better experimental conditions; a thermostatic stage was used to set and control a constant temperature, the analysing procedure was improved by adding strict objective criteria for qualification of the vesicle as a whole as well as for acceptance or rejection of a given contour of the sequence of recorded images and the white noise contribution to the amplitudes of thermal shape fluctuations was R3 evaluated and taken into account. We have given short description of the improvements made and for each of them cited the corresponding articles for detailed explanation. (Lines 181–184; 187–193).
The observed fluctuations of the membrane do not destabilize it.