Produces a dynamic model for chemical evolution rather than traditional deterministic, static, systems that requires centralised information to direct evolution, such as a constant gradient.
Note 4 presents a very important issue: why the inner chemical environment is different from the external one, if the running chemical reactions are the same? The presence of particular features of the vesicle interior or of entrapped catalysts seem a too easy and ad hoc hypothesis. A useful reference proposing an explanation of this symmetry breaking (without requiring different conditions in internal and external environments) could be “R. Serra, M. Villani Mechanism for the formation of density gradients through semipermeable membranes Physical Review E 87, 2013”.