We used a fluorescently tagged Rab11 because the anti-Rab11 antibodies were not sufficient to label recycling endosomes. However, IFITM3 is detected at native levels and this compromise allowed us to evaluate its localization. Overexpressed IFITM3 was only used for the live cell microscopy shown in the movie, which has now been removed (see reply to reviewer 1).
Figure 7: I am not sure that this figure is a useful addition to the paper as it suffers from exactly the problems that the authors claim to overcome in their study, i.e., the use of overexpressed IFITM3 and an overexpressed Rab11 marker protein, both of which may show unnatural localization due to the overexpression.