In the future study, this would be taken into accounted.
I do not understand why soil samples were 0.3 mm sieved. Why? The active soil components (lime, sand, clay) where microorganisms live and organic carbon is accumulated is just less than 2mm. Consequently you excluded an important part of the active soil from your study, therefore your resuts are very limited.R： Moreover, in the subsequent sentence is reported: "The soil was 0.25 mm-sieved to remove large particles; and then weighed series of 10 g aliquots into 250 mL brown jars" Did you sieve the soil twice at 0.3 mm and then 0.25 mm?