The immunoreactive bands were visualized using an enhanced chemiluminescence (Western Lightning, PerkinElmer, Inc., Waltham, MA, USA) and analyzed with ImageQuant LAS 500 (semi-auto mode; GE Healthcare Japan, Tokyo Japan) (Page 4, lines 136-137).
Line 131. On what device was ECL light detected? What were the device settings? How was pixel saturation dealt with?