The present study was examined in terms of autophagy and apoptosis in the antitumor effects of TTField and sorafenib.
In the IHC experiment for PARP, the authors have incubated with primary antibody only for 30 min? Is that timing enough to get protein expression? What source of secondary antibody was used? In addition to IHC, I suggest performing western blot using PARP antibody where the full length and cleaved bands are observed in the same blot.