p5, Figure 1; Figure 1 was reproduced by using the UPGMA clustering method instead of the NJ clustering method according to the suggestion of the reviewer, then, NitM from α-cyanobacteria and β-cyanobacteria form clearly distinct groups. “using the UPGMA (Unweighted Pair Group Method with Arithmetic mean) clustering method of ClustalX.” was added in the legend of Figure 1.
Line 40: Line 120: From the tree as it is, one can see that NitM from β-cyanobacteria form a distinct clade. However, NitM from α‐cyanobacteria are not grouped in one clade. It is possible that another outgroup sequence or different methods for alignment and tree construction will help. Line 122: What methods were used for creating alignment and phylogenetic tree (for example, neighbor-joining)?