We thank the reviewer for further comments and hope that we can clarify the issues as given below. We have made changes in the manuscript (marked red) and added one new reference (ref. [35]).
It is also not clear about the “thermally induced shape fluctuations”, such as the temperatures and incubation length and etc., especially this is a hyperthermophilic archaeal membrane vesicle, which would be expected to be more thermostable than those eukaryotic ones. The observed fluctuations of the membrane do not destabilize it. However, the comment of the reviewer is notable and we state in the conclusion of the manuscript (Lines 229–232) that at room temperature the thermophilicity is not necessarily related to the bending elasticity.