Thanks for your advice, we have added the specific parameters and modified them as follows:” When the collection was completed, the samples were taken back to the laboratory and stored first in the refrigerator (‒20°C). Then, the feces were freeze‒dried (under vacuum conditions, the vacuum freeze drier temperature is ‒40°C to ‒50°C for 48 h) after measuring the weight with a vacuum freeze dryer.” Point 4.
Page 3, lines 116 and 120: Please, add technical details about:lyophilization of food samples. Freeze-drying conditions of feces.