We thank the reviewer for raising this point. However, to respond to this question appropriately, we currently can provide only the results from proliferation assays for one cell line model (FLO1-EV vs FLO1-MACC1) over the time period of 72 h. This assay was with no statistically significant difference in cell proliferation between FLO1-EV vs FLO1-MACC1 (see Figure 4). Figure 4: Proliferation of FLO1 EV and FLO1 MACC1 over 72 h. Analysis of in vitro proliferation assay. Unfortunately, the short revision period of 10 days limits the generation of additional data sets for proliferation in further tumor cell lines.
The authors argue that MACC1 expression did not significantly influenced cell proliferation in the two cell line models. However, cell proliferation was analyzed only in a 24-hour long period even though most cancer cells have a doubling time around 24 hours. In order to draw a conclusion about the proliferation effect a longer (48 or 72 hours) measurement is necessary.