The statistical analysis were conducted as follows: All experiments were conducted in triplicate. The UNIVARIATE procedure of SAS was used to test the normality of the data before any further analyses were carried out. Data were subjected to ANOVA using the MIXED procedure of SAS (SAS 9.2, SAS Institute Inc., Cary, NC). Mean ± standard error (S.E.) were reported, and means were separated by LSD multiple comparisons. Differences were accepted as statistically significance at P < 0.05.
It appears for the first time on Page 1, Line 17; The hot water extraction conditions were tested before?