A number of improvements of the experimental procedure for the thermally induced shape fluctuation method were used in this study: the stroboscopic illumination was improved on several steps to achieve a better experimental conditions; a thermostatic stage was used to set and control a constant temperature, the analysing procedure was improved by adding strict objective criteria for qualification of the vesicle as a whole as well as for acceptance or rejection of a given contour of the sequence of recorded images and the white noise contribution to the amplitudes of thermal shape fluctuations was R3 evaluated and taken into account. We have given short description of the improvements made and for each of them cited the corresponding articles for detailed explanation. (Lines 181–184; 187–193).
I also have questions and comments for acceptance. The list of comments is as follows. In Page 1, Lines 27–28, the author described “the experimental set-up was improved …”. However, the authors have already reported the measurements of bending elasticity using stroboscopic illumination in author’s previous papers (such as Genova et al., J. Optoelectro. Adv. Mat., 2005). Describe how the experimental set-up was improved in this paper more precisely.