We appreciate the recommendation and added the required references as below. We have these specimens of strains available in our lab to study. Therefore, we used. We also updated the standard ciprofloxacin and miconazole with Ceftriaxone and Amphotericin-B and corrected the reference the number of Salmonella Typhi (K-323130) bacteria. We are showing our sincere apology for such mistakes. The changes are added as follows, 2.1.3. Antimicrobial activity assay In vitro antimicrobial activity of synthesized ligands was evaluated by agar disc diffusion method [40]. Mueller Hinton Agar (MHA) media (HIMEDIA, India) was used as a control medium for testing against bacteria and Potato Dextrose Agar (PDA) media (HIMEDIA, India) was used for fungal strain. After preparation, the MHA and PDA medias were incubated for 24 h and contaminations were checked. After incubation, the test organism was inoculated using sterile cotton bar on media. The sample discs were put gently on pre-inoculated agar plates and aerobically incubated for 24 h at 37 °C for antibacterial and for 48 h at 26 °C for antifungal assay. Dimethyl sulfoxide (DMSO) was used as control. Each disc was loaded with 25 µL of sample solution in DMSO containing 300 µg of synthesized compounds. 10 µL of ceftriaxone and amphotericin-B solutions containing 50 µg each in DMSO were loaded per disc for antibacterial and antifungal assays as positive control, respectively. The diameter of the inhibition zones in mm circling the disc were measured. Two gram-positive Staphylococcus aureus (cars-2) and Bacillus Megaterium (BTCC-18), two gram-negative Escherichia coli (carsgn-2) and Salmonella Typhi (K-323130) bacteria, and two fungal strains Trichoderma harzianum (carsm-2) and Aspergillus niger (carsm-3) were used in this study.
We also updated the standard ciprofloxacin and miconazole with Ceftriaxone and Amphotericin-B and corrected the reference the number of Salmonella Typhi (K-323130) bacteria.