While LDH-release or MTT assay are frequently used techniques and might be preferred by some investigators, other approaches assessing cell viability might be more popular by others, dependent on particular experimental settings of a particular study. Figure 2 (for referee inspection only) demonstrates that Alamar blue cell viability assay and Trypan blue live/dead quantification provide results that are identical to those obtained in the cell proliferation protocol used in our study. We believe that the latter protocol is the most suitable experimental approach for our study since it allows distinguishing between cell lysis, cytotoxisity and cytostatic effects (i.e. between cytolysins, cytotoxins and cytostatic agents) in a single experiment. The techniques and algorithms used by the CellDrop relies on accurate and unbiased measurements, akin to previously published techniques[2,3].
The techniques and algorithms used by the CellDrop relies on accurate and unbiased measurements, akin to previously published techniques[2,3].