Thanks for your advice, we have added the specific parameters and modified them as follows:” When the collection was completed, the samples were taken back to the laboratory and stored first in the refrigerator (‒20°C). Then, the feces were freeze‒dried (under vacuum conditions, the vacuum freeze drier temperature is ‒40°C to ‒50°C for 48 h) after measuring the weight with a vacuum freeze dryer.” Point 4.
Finally, the digest was analyzed by HR‒ICP‒MS.