We appreciate the recommendation and agree with the reviewer that the computational and experimental results of antimicrobial activity differ to some extent in this part of the study and required further investigation to understand why such activity differs to the ligands by other means in the future. In this study, we successfully synthesized the novel ligands and studied its spectroscopy with other probable studies. Fortunately, we have found outstanding results during the chemo-sensor study and hopefully, we could be able to show the excellent usage of the ligands as a chemo-sensor in near future. Also, we are planning to do a fluorescence imaging study of protein-ligands.
10 µL of ceftriaxone and amphotericin-B solutions containing 50 µg each in DMSO were loaded per disc for antibacterial and antifungal assays as positive control, respectively.