PGRMC1 is known to be Sumoylated or ubiquitinylated and then degraded by the proteasome pathway, which lead to higher molecular weight species (PMID: 31067491).
Lines 88-98. Cell culture. What characterization was performed to confirm the identity of cells as ESCs? In general, there is very little detail on these primary cultured lines. How long are they held in culture? Are they stored cryogenically? How are they re-seeded into culture?