Other important autophagy and degradation markers like Beclin1 and P62 need to be shown to reflect the regulatory mechanism of TTFields, as well as for the combined treatment with Sorafenib.
The authors claim that cytotoxicity was measured “by cell counting using iCyt EC800 (Sony Biotechnology) 123 flow cytometer, and expressed as a percentage relative to the control.” Does this imply that they counted the live cells and plotted the final data as percentage of untreated controls (as figure 1 suggests). However, is this a real cytotoxicity or a cell growth inhibition? Did they measure the adherent cells after trypsinization. Were the cells from supernatant counted (where are probably the majority of dead cells)? There is a big difference between a therapy which kills vs a therapy which induces a cellular arrest.