Concomitant TTFields with Sorafenib Enhances Treatment Efficacy in Vivo: “Tumor histology and immunostaining for beclin-1 and LC3, GRP78, and cleaved PARP were performed to examine autophagy, ER stress, and apoptosis levels, respectively.
4) Since Annexin V/7-ADD data was not convincing and the authors observed the minor increase of apoptotic cells after HCC cells were treated with combination of TTFields and sorafenib ( when compared to the cells treated with TTFiealds and sorafenib alone), I suggest to run the WBs to examine the expression of the cleaved forms of PARP and caspase-3 ( for both HCC cell lines). This might be helpful and make the in vitro data more relavant with the data shown in vivo.