Since the bottles containing the Martian Analog soils were autoclaved before use, they should be sterile. We also made a separate test to check for sterility after autoclaving the Martian Analog soils, which has been negative (meaning no contamination could be detected via plating of the autoclaved JSC Mars-1A regolith on TYG medium).
Line 57: Are you sure that the Martian Analog soils were sterile before using?