genes. We feel sorry for the unclear statement. In our study, we used the amino acid sequence of OfSPL11 to compute the protein model. Secondary structure predictions were conducted on the PRABI (http://www.prabi.fr/spip.php?page=sommaire) protein structure prediction server. The ExPASy (http://web.expasy.org) was used to analyze the molecular weight, theoretical isoelectric point, and other physiochemical properties of the translated protein. Similar methods of operation and efforts can be found in other articles (Islam, 2021). Once again,we sincerely thank you for the recommended method, which we will use in future studies. Islam, M.D.; Rahman, M.M.; Rahman, M.M. ; Jin, X.J. ; Sun, L.L. ; Zhao, K.; Wang, S.; Sikdar, A.; Noor, H.; Jeon, J.S. ; Zhang, W.J. ; Sun, D.Z. In Silico and Transcription Analysis of Trehalose-6-phosphate Phosphatase Gene Family of Wheat: Trehalose Synthesis Genes Contribute to Salinity, Drought Stress and Leaf Senescence. 2021, 12, 1652. https://doi.org/10.3390/genes12111652 Point 2: Auhtors report that Actin was used as housekeeping gene.
Why the authors do not consider to use aminoacidic sequence to compute a protein model (e.g., by using gromacs)?