We selected the specific supernatant volumes used to reach a similar lethal dose to compare the protection capability and efficiency of the liposomal nanotraps. However, as the immune cell lines have different sensitivities depending on the toxin profile of the tested strain, a similar lethal dose corresponds to different supernatant volumes. We used the results displayed in Figure 1 of the manuscript to determine which volume to use. We agree that the way we presented it can lead to confusion and we replaced the supernatant volume values by LD% values. The following text was added to the material and method section: The added supernatant volume was determined based on the toxicity assay results and was used either at saturating dose (lethal dose >90, LD>90) or at non-saturating dose (LD60-90) to study minor toxin activities. We agree that the distinction between saturating (LD>90) and non-saturating (LD60-90) was confusing. To clarify our process we did add clarifications throughout the results part and we added a panel in figure 2 to include results obtained after challenge of LD>90 of GGS 5804 supernatant.
Carlsen J, Cömert C, Bross P, Palmfeldt J. Optimized High-Contrast Brightfield Microscopy Application for Noninvasive Proliferation Assays of Human Cell Cultures.