As it was explained in the text our experimental method is developed for application to almost spherical vesicles. Vesicles with diameters between 20 and 40 micrometers were considered, whose deviations from spherical shape (fluctuation of the radius) were small in comparison with the mean sphere radius. We added the information concerning the shape and the size of the studied objects and added pictures of the equatorial cross section of a fluctuating vesicle (new Figure 3), as seen under the phase contrast microscope.
However, the comment of the reviewer is notable and we state in the conclusion of the manuscript (Lines 229–232) that at room temperature the thermophilicity is not necessarily related to the bending elasticity.