We completely agree with the reviewer that NR2F1 expression in cancer cells and CAFs should be investigated not only in primary but also in metastatic breast cancer using single-cell sequence in order to prove that NR2F1 expression in CAFs affects late recurrence. The main finding of this study is that NR2F1 is predominantly expressed in CAFs rather than in all other cell types in the TME, and we do not intend to claim that CAF-expressed NR2F1 regulates dormancy. Further, we do not have access to single-cell sequence cohorts of metastatic breast cancer tumors, but it is of our interest, and this will be our future direction. In response to the reviewer, we added the following sentences in the discussion section.
Cancer mutation rates, immune responses, and immune cell infiltrations were lower in high NR2F1 tumors whereas the infiltration of stromal cells including cancer-associated fibroblasts (CAFs) was higher.