We thank the reviewer for pointing this was not clear. As mentioned in the statistical analysis sub-section within the methods part, all in vitro experiments were repeated at least 3 times, and so depicted values are mean (N ≥ 3) ± SEM. We have now added this also to the legends of figures 1, 2, and 3. Per the in vivo study, as was mentioned in the methods (sub-section 2.8), 52 animals were included in the study. The specific numbers in each treatment group are now mentioned in the results (sub-section 3.4): “During the treatment period, average tumor volumes of control animals (n = 11) increased 5.9-fold (Figure 4b and 4c; and Figure S3 for tumor images). For animals treated with TTFields (n = 15) or sorafenib (n = 10), tumor growth was significantly lower, 3.3- and 2.3-fold, respectively. In the TTFields-sorafenib combination group (n = 16), a 1.6-fold increase in tumor volume within the treatment period was observed, a growth significantly lower than that for control or for each treatment alone.” It is also mentioned in the legend of figure 4: “Rats (n = 52) were inoculated orthotopically with rat HCC N1S1 cells, and treated with sham-vehicle (n = 11), TTFields alone (n = 15), sorafenib alone (n = 10), or TTFields + sorafenib (n = 16), according to the depicted timeline (a).” Point 3: LC3 is increased in all the groups except in control tissue, however, cell death was increased only in combined group.
Point 9: Was Mycoplasma testing done routinely?