Appropriate human ethics approvals were obtained.
Nine PGRMC1 lysines are known to be ubiquitinated (https://www.phosphosite.org/proteinAction.action?id=5744&showAllSites=true). Individual mutation of these residues could stabilise PGRMC1 levels to reduce decidualization if miR-98 is only partially responsible for reducing PGRMC1 levels. In fact, proteasomal degradation could be much more important that miRNA reduction of the transcript in the reduction of PGRMC1 levels during decidualization. It is important to determine whether these mechanisms are both operating. At the very least this mechanism merits detailed discussion in that section. PGRMC1 protein instability could be the dominant effect involved, with miRNA transcript regulation playing only a minor part.