Western blotting for PGRMC1 in figures 2, 3, and 4 have been replaced in the data with the whole molecular weight range. As you pointed out, PGRMC1 is known to be modified by sumoylation or ubiquitinylation which results in high molecular weight. The right panel shows the western blotting of PGRMC1. There are some higher molecular bands (white arrowhead) in lysates of ESCs, however, the band’s intensity was not changed by PGRMC1 siRNA treatment. Therefore, we believe that these high molecular bands detected in our experiment were non-specific bands.
Nine PGRMC1 lysines are known to be ubiquitinated (https://www.phosphosite.org/proteinAction.action?id=5744&showAllSites=true).