Stroboscopic illumination measurements of GVs and theoretical models about shape fluctuation of GVs revealed that the archaeal lipid-based GVs showed the almost same value of bending elasticity modulus with synthetic lipid-based GVs.
However, the comment of the reviewer is notable and we state in the conclusion of the manuscript (Lines 229–232) that at room temperature the thermophilicity is not necessarily related to the bending elasticity.