For the in vitro experiments with TTFields (sub-section 2.3) we tried not to elaborate too much and referenced previous work describing all details. We understand that we have cut off too many details, and are happy to add them “HepG2 and Huh-7D12 cell suspensions (500 µl, 25 x 103 cells/plate) were placed as a drop in the center of 35-mm inovitro™ dishes composed of high dielectric constant ceramic (lead magnesium niobate–lead titanate [PMN-PT]), with two perpendicularly pairs of transducer arrays printed on their outer walls. Cells were incubated overnight at 37 °C to allow attachment to the dish, and then 2 ml of fresh media were added.” Regarding the number of rats included in the final analysis, we apologize this information was not clear. The 52 rats mentioned in the text were in fact the actual final number of animals in the analysis, since “all rats reached the required usage limit of ≥18 h/day”, as is now explicitly stated in the results sub-section 3.4.” Point 2: What is puzzling in this investigation is showing functional data using two human cell lines and in vivo data using rat cells.
Author Response Please see attachment Author Response File: Author Response.docx