We show indirect immunofluorescence analysis with anti-IFITM3 and anti-NP antibodies of HSAEpCs in Figure S6. No unspecific signal is detected in the cytosol of HSAEpCs, indicating that the observed cytoplasmic NP stain is specific. Nuclear NP stain is detected at 10 h p.i., and this may indeed indicate delayed replication compared to A549 cells as suggested by the reviewer, but no direct side-by-side comparison of replication kinetics in different cells was performed.
b. The NP stain in HSAEpCs is only shown to be cytoplasmic, inclusion of an uninfected control would demonstrate specificity of the staining. At later timepoints is the NP stain nuclear as expected, is this simply a delay in replication?