As you pointed out, we did not explain the relationship between PGRMC1 stability and post-transcriptional regulation of the protein. We have added the following description (page 9, lines 304-308). ”These reports suggested that PGRMC1 protein levels can be modulated by changes in the rates of transcription, translation, and degradation. Therefore, further studies would be needed to clarify the possible involvement of the post-translational modification and degradation that may regulate PGRMC1 protein stability in ESCs during decidualization.”
The salient point concerning the ubiquitination events is that they are correlated for other proteins with proteasome-mediated degradation. Sabbir’s results (ref 31) imply that this also holds for PGRMC1. The new text does not conceptually link PTMs with PGRMC1 protein stability, which is the only reason they should be mentioned. To be clear, protein levels can be mediated by altered rates of transcription, translation, or degradation. This new text should be discussing the possibility of the latter also contributing to the observed effects, as well as the effects of mRNA level that the paper pursues.