Western blotting for PGRMC1 in figures 2, 3, and 4 have been replaced in the data with the whole molecular weight range. As you pointed out, PGRMC1 is known to be modified by sumoylation or ubiquitinylation which results in high molecular weight. The right panel shows the western blotting of PGRMC1. There are some higher molecular bands (white arrowhead) in lysates of ESCs, however, the band’s intensity was not changed by PGRMC1 siRNA treatment. Therefore, we believe that these high molecular bands detected in our experiment were non-specific bands.
2. Line 158/Fig. The western blot (also Fig.3) result must show the entire molecular weight range (e.g. in supplemental data), including MW markers. PGRMC1 is known to be Sumoylated or ubiquitinylated and then degraded by the proteasome pathway, which lead to higher molecular weight species (PMID: 31067491). The latter could well contribute to alterations in PGRMC1 abundance cited by this study. It is important that the involvement of the ubiquitin pathway contribution be either quantified or discarded in well controlled experiments to justify the title of the paper.