As the referee suggested, we have added data for the bending elasticity modulus of another lipid type—POPC lipid membrane. Also, we have added a new figure (Figure 3).
Second Round of Evaluation Round 2: and Author Response It is also not clear about the “thermally induced shape fluctuations”, such as the temperatures and incubation length and etc., especially this is a hyperthermophilic archaeal membrane vesicle, which would be expected to be more thermostable than those eukaryotic ones.