The protein samples were separated by SDS-PAGE (5-20% gradient gel) and transferred electrophoretically to polyvinylidene difluoride membranes (8 cm x 8.5 cm) for 60 min at constant current of 128 mA using a semi-dry transfer system (ATTO, Tokyo Japan). These conditions have been added to the Materials and Methods section (Pages 3 and 4, lines 127-130).
8) Lines 121-133. What percentage acrylamide were the SDS PAGE gels. For what length of time and at what voltage settings was electrophoretic transfer to PVDF? In what device?