thank you for this comment: we added the proliferation analyses by MTT into the method section page 5: Additionally, proliferation for FLO-1/EV and FLO-1/MACC1 was analyzed over 72 h by MTT. 4x103 cells were plated into 96-well-plates and were allowed to accommodate for 24 h. after 48 and 72 h formazan crystals were dissolved in 150 μl of DMSO and the absorption was measured at 560 nm in the absorbance reader (Tecan infinite 200 PRO). Each cell proliferation experiment was performed in triplicates. We added the results of the MTT analyses into the results section page 11: The additional analysis of the proliferation over 72 h showed no significant differences between FLO-1/EV (100% ± 4.886) and FLO-1/MACC1 (112.3% ± 12.24; p=0.113) (see supplement Figure S3). We added the MTT plot as figure S2 into the supplement section Figure S2: Proliferation of FLO1 EV and FLO1 MACC1 over 72 h. Analysis of in vitro proliferation assay.
[5] If possible, please use a higher resolution version of Figures 3F and 3I (the legends are blurry and hard to read in the current version).