The bending elasticity modulus depends on the temperature below and near the phase transition temperature, but far above the phase transition temperature (this is the case in our experiment) the bending elasticity modulus is practically constant. See: Temperature and Chain Length Effects on Bending Elasticity of Phosphatidylcholine Bilayers. Fernandez-Puente, I. Bivas, M. D. Mitov and P. Meleard, Europhys. Lett., 28, 181 (1994). We have added this text to the Discussion (Lines 234–237) and also added the reference to the reference list (ref.
Stroboscopic illumination measurements of GVs and theoretical models about shape fluctuation of GVs revealed that the archaeal lipid-based GVs showed the almost same value of bending elasticity modulus with synthetic lipid-based GVs.