The IFITM3 images were acquired in the cytosol distant from the nucleus and absent from the plasma membrane. We included this information in the respective figure legend of Figure 3 and 7. Figure 3: “STED images (raw data) show representative subcellular regions located in the cytosolic part distant from the nucleus and absent from the plasma membrane as used for cluster analysis.” Figure 7: “Images show regions located in the cytosolic part distant from the nucleus and absent from the plasma membrane.” d.
Furthermore, the authors need to examine the literature more carefully, as previous studies have indeed shown that IFITM3 knockdown in A549 cells increases influenza virus infection, e.g., Lin, Cell Reports, 2013.