We thank the reviewer for pointing out this issue. Quantification of the IHC images was done automatically. The whole slide was scanned, and the CaseViewer software was used to exclude non-tumor areas. The signals of the stained protein and the nuclei were resolved by color deconvolution and quantified separately using the FIJI software (ImageJ) software. Average signal per cell or percent of positive cells was calculated. As the reviewer pointed out, the high magnification images we chose to show do not correctly reflect the quantification performed by the software, and we have now replaced them with better representative fields of the slides.
Images of tumors within the livers of the rats treated with sham heat (control rats), TTFields, sorafenib, or TTFields plus sorafenib.