Enunciate what kind of role(s) PGRMC1 may be involved in (up/down regulation, when, how?).
Lines 178-9. If PGRMC1 down-regulation is critical, it should be possible to perturb decidualization by expression of an exogenous PGRMC1 protein. This could be accomplished by making an expression plasmid encoding a codon-altered PGRMC1 gene, and cotransfecting this in a controlled matrix design with siRNA. If siRNA-resistant PGRMC1 levels are able to inhibit decidualization it would provide convincing evidence in favour of the hypothesis that lowered PGRMC1 is required for decidualization, and is perhaps even a trigger. A codon-altered PGRMC1 expression vector would cost just several hundred dollars from a provider such as Genscript, so the experiment is eminently achievable.