Bending modulus is a material constant which does not depend on the size and shape of the object, but on the composition of the vesicle membrane and aqueous solution used (Lines 121–123 in the new version). We have inserted a definition of the membrane bending modulus (Lines 115–116) while the text regarding this issue extends between Lines 112–128.
Stroboscopic illumination measurements of GVs and theoretical models about shape fluctuation of GVs revealed that the archaeal lipid-based GVs showed the almost same value of bending elasticity modulus with synthetic lipid-based GVs.