The IFITM3 blot shows wide variability with no pattern from one hour to the next, and yet the conclusion is that there is no change in IFITM3 levels during this time period.
Lines 67-73 The inhibition of virus membrane fusion by IFITM3 has been attributed to the presence of a palmitoylated amphipathic helix within IFITM3 (Chesarino, EMBO Reports, 2017). This helix and neighboring palmitoylation sites are among the most highly conserved residues among IFITMs from all species. Additionally, the role of cholesterol in IFITM3’s mechanism of action has been largely disproven by the field, and reference 52 seems to have been misused in line 73.