As it was explained in the text our experimental method is developed for application to almost spherical vesicles. Vesicles with diameters between 20 and 40 micrometers were considered, whose deviations from spherical shape (fluctuation of the radius) were small in comparison with the mean sphere radius. We added the information concerning the shape and the size of the studied objects and added pictures of the equatorial cross section of a fluctuating vesicle (new Figure 3), as seen under the phase contrast microscope.
However, not all results seem to be presented. For example, it is not clear what kind of images were recorded, which were used to calculate the kc values, what the sizes/shapes of the fluctuating vesicles looked like. How these observations would be different or similar to other types of vesicles.