The immunoreactive bands were visualized using an enhanced chemiluminescence (Western Lightning, PerkinElmer, Inc., Waltham, MA, USA) and analyzed with ImageQuant LAS 500 (semi-auto mode; GE Healthcare Japan, Tokyo Japan) (Page 4, lines 136-137).
Translational level changes can be due to changed levels of transcription, to altered mRNA stability (such as that proposed here for miR-98), or to altered efficiency of translation of a steady state mRNA pool.