This immunoquantitation method used is described in detail in the reference given (Brown et al. 2008). The reviewer is directed in particular to the Supplementary Methods section. To allow readers to get a better understanding of the method in this manuscript we have revised this part of the Experimental Section, adding extra text to increase clarity concerning the method.
The data are interesting and worth publishing but… (1) I don’t understand how they calculated the protein molar ratios. In the methodology section we learn that they extracted the crude proteins and then performed western analyses using specific antibodies, followed by image analyses. I don’t understand how they converted the western data to read the amount of specific proteins, in fmol, in the various organisms as shown in Figure 2; and used for the calculations in the rest of the paper. (Why are the units provided again in Line 220?). Please provide the reader with the methodology used for the quantification.