We thank the reviewer for this comment, and apologize that the illustration of the timeline was accidentally missing from the submission (we have now added it). The limiting factor for study duration was the well being of the animals. The tumors in the control group were very large, causing stress and weight loss of the animals. The arrays placed on the animals, together with the individual housing needed to prevent wire entanglement, adds even more stress and increases animal weight loss. Overall, it was non-ethical to continue the study further. We have now added this explanation to the discussion section to clarify this limitation of the study: “In the HCC animal model, the acute effects of TTFields and sorafenib were examined. Due to the large tumors developed in the control group and the stress experienced by the animals as a result of the individual housing and motility limitations imposed by the sham and TTFields arrays, longer treatment durations were not feasible.” Point 4: There is no explanation why the most effective dose was 150 kHz and higher does actually decreases the killing.
Also, is there any evidence that TTF may prevent the pretty common resistance to sorafenib observed in clinic?