Thank you for your valuable advice to prove the significance of PGRMC1 downregulation in decidualization. Salsano et al. (19), reported that overexpression of PGRMC1 in ESCs abrogated the decidual markers expression (page 8. Lines 253-254). We will try to examine the effects of enforced expression of codon-altered PGRMC1 which is resistant for siRNA on decidualization in next study.
19) Lines 178-9. If PGRMC1 down-regulation is critical, it should be possible to perturb decidualization by expression of an exogenous PGRMC1 protein. This could be accomplished by making an expression plasmid encoding a codon-altered PGRMC1 gene, and cotransfecting this in a controlled matrix design with siRNA. If siRNA-resistant PGRMC1 levels are able to inhibit decidualization it would provide convincing evidence in favour of the hypothesis that lowered PGRMC1 is required for decidualization, and is perhaps even a trigger. A codon-altered PGRMC1 expression vector would cost just several hundred dollars from a provider such as Genscript, so the experiment is eminently achievable.