HepG2 cells were treated for 6, 24, or 48 hours with 150 kHz TTFields, 3 µM sorafenib, or the two treatments combined, followed by Western blot examination of the autophagy markers beclin-1 and LC3 (d), the ER stress marker GRP78 (e), and the apoptosis marker cleaved PARP (f).
This might be helpful and make the in vitro data more relevant with the data shown in vivo.