CREATE TABLE table_17778 ("Sequencer" TEXT, "Ion Torrent PGM" TEXT, "454 GS FLX" TEXT, "HiSeq 2000" TEXT, "SOLiDv4" TEXT, "PacBio" TEXT, "Sanger 3730xl" TEXT)

What is 454 gs flx when pacbio is 100-500 mb?
SELECT "454 GS FLX" FROM table_17778 WHERE "PacBio" = '100-500 Mb'
SELECT "454 GS FLX" FROM "table_17778" WHERE "PacBio" = '100-500 Mb'